CN115521869A - Radioactive particle in-vitro cell irradiation experiment template based on 3D printing - Google Patents
Radioactive particle in-vitro cell irradiation experiment template based on 3D printing Download PDFInfo
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- CN115521869A CN115521869A CN202211406000.2A CN202211406000A CN115521869A CN 115521869 A CN115521869 A CN 115521869A CN 202211406000 A CN202211406000 A CN 202211406000A CN 115521869 A CN115521869 A CN 115521869A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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Abstract
The invention discloses a radioactive particle in-vitro cell irradiation experiment template based on 3D printing, which comprises a template body, a particle storage mechanism and a culture dish supporting mechanism, wherein the particle storage mechanism and the culture dish supporting mechanism are positioned on the template body; the particle storage mechanism comprises a particle groove printing area located on the template body, a reference line which is located on the particle groove printing area and is arranged in parallel, particle grooves which are arranged along the length direction of the reference line, and particle grooves which are located on the same reference line are arranged at equal intervals. Through the integral type experiment template of 3D preparation, the particle is arranged in the particle recess, and the culture dish supports through the culture dish and supports, guarantees to shine evenly, can print the template of equidimension not simultaneously, change the interval between the particle recess, adjust the figure of particle recess according to different culture dish sizes, experiment dosage requirements. The template meets the experiment requirements, ensures that the irradiation rays received in the culture dish field are uniform, and meets different requirements of the tumor cell radioactive particle in-vitro irradiation experiment.
Description
Technical Field
The invention relates to the technical field of medical experimental equipment, in particular to a radioactive particle in-vitro cell irradiation experimental template based on 3D printing.
Background
The radioactive particle implantation is widely applied to clinical short-distance irradiation treatment at present, is mainly applied to solid tumor implantation treatment, and has obvious curative effect. However, fundamental studies on the mechanism of irradiation therapy within radioactive particles have not been intensively carried out, and the influence of the continuous gamma irradiation of radioactive particles on tumor cells and microenvironment has not been clarified. There is a need to develop relevant studies to specify the unique mechanism of irradiation therapy within the radioactive particles, so as to guide the clinical radiotherapy treatment scheme and dose, and facilitate the wide clinical application of the radioactive particles. In vitro tumor cell irradiation is a common research method for discussing radiotherapy, and due to the influences of factors such as small volume, uneven irradiation and the like of radioactive particles, the experimental requirements of uniform distribution and equal-dose irradiation of the radioactive particles cannot be effectively simulated in the aspect of in vitro irradiation.
The related patents of the related radioactive particle experiment template which are applied at present are as follows: chinese patent CN212800387U is a radioactive irradiation device for cancer cells, the base and the cap are located in a radiation-proof housing, the base is provided with a plurality of culture dishes for placing cancer cells, the bottom of each culture dish is provided with an accommodating groove for accommodating radioactive particles, and the cap is located above the base and covers the culture dish. The design irradiates multiple cell culture dishes simultaneously, and the radioactive particle field areas of different culture dishes are crossed and mutually influenced. The radioactive particle grooves are designed unevenly, so that cells in an irradiation field can not be guaranteed to receive equal-dose ray irradiation, and meanwhile, adjustment can not be carried out according to different dose experiment requirements. The cap covers above the culture dish, effective ventilation cannot be guaranteed, cell culture is extremely large, and experimental effect is easily influenced.
Disclosure of Invention
The invention aims to provide a radioactive particle in-vitro cell irradiation experiment template based on 3D printing, which is used for solving the technical problems in the background technology.
The invention provides a 3D printing-based radioactive particle in-vitro cell irradiation experiment template, which comprises a template body, a particle storage mechanism and a culture dish supporting mechanism, wherein the particle storage mechanism and the culture dish supporting mechanism are positioned on the template body;
the particle storage mechanism comprises a particle groove printing area, a reference line and a particle groove, wherein the particle groove printing area is positioned on the template body, the reference line is positioned in the particle groove printing area and is arranged in parallel, the particle groove is arranged along the length direction of the reference line, and the particle grooves on the same reference line are arranged at equal intervals.
In a preferred embodiment, the particle recess regions are arranged in a circle, and the diameters of the particle recess regions are not unique.
In a preferred embodiment, the distance between two adjacent reference lines is 10mm.
In a preferred embodiment, the particle recesses have a length of 5mm and a width of 1mm, and the spacing of the particle recesses on the same reference line is not unique and is between 5-10 mm.
In a preferred embodiment, the culture dish supporting mechanisms are arranged at four corners of the template body, each culture dish supporting mechanism comprises a supporting column and a supporting strip which is connected with the supporting corners and horizontally arranged, the bottom of each supporting strip is 5mm away from the upper surface of the template body, and the length of each supporting strip is 2mm.
In a preferred embodiment, the particle storage mechanism and the culture dish support mechanism are both made by 3D printing.
The technical scheme of the invention has the beneficial effects that:
through the integral type experiment template of 3D preparation, the particle is arranged in the particle recess, and the culture dish supports through the culture dish and supports, requires according to different culture dish sizes, experiment dosage, and printable not template of equidimension changes interval, particle recess figure between the particle recess according to the demand simultaneously. The template meets the experiment requirements, ensures that the irradiation rays received in the culture dish field are uniform, and meets different requirements of the tumor cell radioactive particle in-vitro irradiation experiment.
Drawings
Figure 1 is a schematic view of the overall structure of the present invention,
FIG. 2 is an enlarged view of the invention at A in FIG. 1.
Description of reference numerals: 1 template body, 2 particle storage mechanisms, 3 particle groove printing areas, 4 reference lines, 5 particle grooves, 6 culture dish supporting mechanisms, 7 supporting columns and 8 supporting strips.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments. The embodiments of the present invention have been presented for purposes of illustration and description, and are not intended to be exhaustive or limited to the invention in the form disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiment was chosen and described in order to best explain the principles of the invention and the practical application, and to enable others of ordinary skill in the art to understand the invention for various embodiments with various modifications as are suited to the particular use contemplated.
As shown in fig. 1-2, the technical solution of the present invention discloses a radioactive particle in-vitro cell irradiation experiment template based on 3D printing, which includes a template body 1, a particle storage mechanism 2 located on the template body 1, and a culture dish support mechanism 6, wherein the particle storage mechanism 2 is used for storing radioactive particles, and the culture dish is supported by the culture dish support mechanism 6 and then located above the particle storage mechanism 2. The particle storage mechanism 2 and the culture dish support mechanism 6 are both made by 3D printing.
The particle storage mechanism 2 comprises a particle groove printing area 3 positioned on the template body 1, a reference line 4 positioned on the particle groove printing area 3 and arranged in parallel, and particle grooves 5 arranged along the length direction of the reference line 4, wherein the particle grooves 5 positioned on the same reference line 4 are arranged at equal intervals. The size of the particle recess printing zone 3 is determined according to the diameter of the culture dish, for example, for a culture dish with a diameter of 100mm, the diameter of the particle recess printing zone 3 is also set to 100mm. The radioactive particles are stored in the particle recesses 5, and one particle recess 5 stores one radioactive particle.
In order to adapt to the shape of the culture dish, the particle groove 5 area is usually set to be circular, and the diameter of the particle groove 5 area is not unique and can be printed to different diameters according to specific experimental requirements.
The length of the particle groove 5 is 5mm, the width is 1mm, and the standard of the radioactive particles commonly used in clinic is 0.8mm multiplied by 4.5mm, so the size of the particle groove 5 is set to be 1mm multiplied by 5mm, and the particles can be smoothly arranged in the particle groove 5 and fixed in position. The distance between two adjacent reference lines 4 is 10mm, and the distance between the particle grooves 5 on the same reference line 4 is not only 5-10 mm. The distance between the particles implanted into the tumor clinically is 5-10mm according to the effective irradiation radius (17 mm) of the radioactive particles, and the distance between the 5 values of the particle grooves can be adjusted by several money according to different metering requirements. The above arrangement can simulate clinical and TPS plans to require that the irradiation rays received in the culture dish field are uniform.
Culture dish supporting mechanism 6 sets up at four angles of template body 1, and culture dish supporting mechanism 6 is connected and the support bar 8 of level setting including supporting angle 7 and with supporting angle 7, and 8 bottom distances of support bar 5mm from template body 1 upper surface, and 8 length of support bar are 2mm. The culture dish passes through support bar 8 and supports, and 8 bottoms of support bar are 5mm apart from 1 upper surface of template body, and support bar 8 sets up to 2mm, accords with clinical and cell experiment conventional requirement, and guarantees that the culture dish is steady fixed and do not shelter from the ray. At the same time, the culture dish completely overlaps the circular radioactive particle placement area.
It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by one of ordinary skill in this and related arts based on the embodiments of the present invention without creative efforts, shall fall within the protection scope of the present invention. Structures, devices, and methods of operation not specifically described or illustrated herein are not specifically illustrated or described, but are instead contemplated to be practiced in the art by those skilled in the art.
Claims (6)
1. The utility model provides a radioactive particle in vitro cell irradiation experiment template based on 3D prints which characterized in that: the particle storage device comprises a template body, a particle storage mechanism and a culture dish supporting mechanism, wherein the particle storage mechanism and the culture dish supporting mechanism are positioned on the template body;
the particle storage mechanism comprises a particle groove printing area, a reference line and a particle groove, wherein the particle groove printing area is positioned on the template body, the reference line is positioned in the particle groove printing area and is arranged in parallel, the particle groove is arranged along the length direction of the reference line, and the particle grooves on the same reference line are arranged at equal intervals.
2. The radioactive particle in-vitro cell irradiation experiment template based on 3D printing as claimed in claim 1, wherein: the particle groove regions are arranged in a circle, and the diameters of the particle groove regions are not unique.
3. The radioactive particle in-vitro cell irradiation experiment template based on 3D printing as claimed in claim 1, wherein: the distance between two adjacent reference lines is 10mm.
4. The radioactive particle in-vitro cell irradiation experiment template based on 3D printing as claimed in claim 1, wherein: the particle grooves are 5mm long and 1mm wide, and the spacing between the particle grooves on the same reference line is not unique and is between 5 and 10mm.
5. The experimental template for radioactive particle in vitro cell irradiation based on 3D printing as claimed in claim 1, wherein: culture dish supporting mechanism sets up four angles of template body, culture dish supporting mechanism include the support column and with the support bar that the support angle is connected and the level sets up, support bar bottom distance template body upper surface 5mm, support bar length are 2mm.
6. The radioactive particle in-vitro cell irradiation experiment template based on 3D printing as claimed in claim 1, wherein: the particle storage mechanism and the culture dish supporting mechanism are both manufactured through 3D printing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211406000.2A CN115521869A (en) | 2022-11-10 | 2022-11-10 | Radioactive particle in-vitro cell irradiation experiment template based on 3D printing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211406000.2A CN115521869A (en) | 2022-11-10 | 2022-11-10 | Radioactive particle in-vitro cell irradiation experiment template based on 3D printing |
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| Publication Number | Publication Date |
|---|---|
| CN115521869A true CN115521869A (en) | 2022-12-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211406000.2A Pending CN115521869A (en) | 2022-11-10 | 2022-11-10 | Radioactive particle in-vitro cell irradiation experiment template based on 3D printing |
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|---|---|
| CN (1) | CN115521869A (en) |
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2022
- 2022-11-10 CN CN202211406000.2A patent/CN115521869A/en active Pending
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