CN210875398U - Microfluidic chip and microfluidic chip assembly - Google Patents

Abstract

A microfluidic chip and a microfluidic chip assembly are provided. The micro-fluidic chip comprises a sample introduction unit and a flow guide hole. The sample injection unit comprises a bottom plate and a side wall, and a cavity with an opening is formed by the bottom plate and the side wall in a surrounding mode; the guide hole penetrates through the bottom plate of the sample injection unit along the direction perpendicular to the main plate surface of the bottom plate. The bottom plate is including extending to the guiding gutter in guiding hole towards open-ended one side, and the degree of depth of guiding gutter is 0.1 ~ 2 millimeters, and the width of guiding gutter is 0.1 ~ 2 millimeters. The micro-fluidic chip adopts a flow guide groove capable of forming capillary force to play a continuous drainage role on the sample in the sample introduction unit, so that the sample is easier to collect, and the collected sample amount is larger.

Description

Microfluidic chip and microfluidic chip assembly

Technical Field

At least one embodiment of the present invention relates to a microfluidic chip and a microfluidic chip assembly.

Background

The microfluidic chip is a chip that integrates basic operation units related to sample preparation, separation, reaction, detection and the like in the biological or chemical field into a few square centimeters or even smaller to construct a biological or chemical analysis platform, thereby realizing various functions of a conventional biological or chemical laboratory. The microfluidic technology has the characteristics of small sample volume, high integration level and easy realization of automatic control and high-flux analysis, so that the biochemical detection on the microfluidic chip is more convenient and faster than the conventional biochemical detection, and the cost is low. The micro-fluidic chip generally completes the reaction automatically through a matched instrument, the internal reaction process is completely controllable, the technical requirements on users are reduced, and the human errors of detection are reduced, so that more accurate detection data are obtained.

The biochemical detection is to analyze blood or other body fluids through various biochemical reactions or immunoreactions, measure the contents of indexes such as enzymes, saccharides, lipids, proteins and the like in vivo, and provide important basis for disease diagnosis for clinicians. Biochemical tests are routine tests in hospitals and are mainly performed by large-scale biochemical analyzers. Although the full-automatic large-scale biochemical analyzer has realized the full integration and the full automation of the whole detection process, the biochemical analyzer is expensive, large in size, complex in operation, and needs professional detection personnel to perform operation and daily maintenance, and the detection time period is long, so that real-time and rapid on-site timely detection cannot be performed. In addition, the biochemical reaction kit is expensive. Therefore, the portable, simple-to-operate and fast and intuitive detection device becomes the key point of domestic and foreign research.

SUMMERY OF THE UTILITY MODEL

An embodiment of the utility model provides a micro-fluidic chip and micro-fluidic chip subassembly, the utility model provides a micro-fluidic chip adopts the guiding gutter that can form capillary force, plays to advancing the sample in the kind unit and lasts the drainage effect for the sample is collected more easily, and the sample volume of collecting is great.

At least one embodiment of the utility model provides a micro-fluidic chip, including advancing kind unit and water conservancy diversion hole. The sample injection unit comprises a bottom plate and a side wall, and a cavity with an opening is formed by the bottom plate and the side wall in a surrounding mode; the flow guide hole penetrates through the bottom plate of the sample injection unit along the direction perpendicular to the main plate surface of the bottom plate. The bottom plate towards one side of open-ended is including extending to the guiding gutter of guiding hole, the degree of depth of guiding gutter is 0.1 ~ 2 millimeters, just the width of guiding gutter is 0.1 ~ 2 millimeters.

In some examples, the number of the flow guide grooves is plural, the flow guide hole is located at the center of the bottom plate, and the plural flow guide grooves surround the flow guide hole.

In some examples, the plurality of flow guide grooves includes a plurality of first bar-shaped flow guide grooves radially distributed around the flow guide hole, and each of the first bar-shaped flow guide grooves extends to the flow guide hole.

In some examples, the first bar groove has a depth at a position close to the guide hole greater than a depth of an end thereof away from the guide hole, and the depth of the first bar groove gradually changes along an extending direction of the first bar groove.

In some examples, two sidewalls forming the first bar-shaped guide channel extend into edges of the guide hole.

In some examples, the two sidewalls forming the first bar-shaped guide groove extend to the edge of the guide hole.

In some examples, the cross section of the flow guide hole, which is taken by a plane parallel to the main plate surface of the bottom plate, is a circle, and the diameter of the flow guide hole is 0.2-2 mm.

In some examples, two sidewalls forming the first bar-shaped guide groove extend to a position outside an edge of the guide hole, and a distance between an end of the sidewall near the guide hole and the edge of the guide hole is not greater than 5 times a diameter of the guide hole.

In some examples, the width of the first linear guide groove is 0.3-0.5 mm, and the number of the first linear guide grooves is 15-18.

In some examples, the first linear guide groove has a length of 1.5-15 mm.

In some examples, a cross-sectional shape of an end of the first bar-shaped guide groove remote from the guide hole, taken by a plane parallel to the bottom plate, is a circular arc.

In some examples, the plurality of flow channels further includes at least one ring of annular flow channels surrounding the flow guide hole, and the at least one ring of annular flow channels communicates with the first linear flow channel.

In some examples, the plurality of flow guide grooves further includes a plurality of second strip-shaped flow guide grooves radially distributed around the flow guide hole, the second strip-shaped flow guide grooves have a length smaller than that of the first strip-shaped flow guide grooves and are communicated with the at least one ring of annular flow guide grooves.

In some examples, each of the second strip-shaped guide grooves is located between two adjacent first strip-shaped guide grooves and extends from a position, far away from the guide hole, of any one of the at least one circle of annular guide grooves to the annular guide groove and is communicated with the annular guide groove.

In some examples, the microfluidic chip further comprises: the micro-channel is positioned on one side of the bottom plate of the sample injection unit, which is far away from the opening, and is communicated with the flow guide hole; and at least one reaction tank connected with the end part of the micro-channel far away from the diversion hole.

An at least embodiment of the utility model provides a micro-fluidic chip subassembly, including above-mentioned micro-fluidic chip and cover the open-ended gland of appearance unit advances, the gland towards the material of the at least part of the bottom plate of appearance unit is elastic material. The utility model provides a micro-fluidic chip subassembly can be through applying pressure so that the sample filters fast for the sample that is located the appearance unit through adopting the gland that has elastic material.

In some examples, a side of the diversion trench facing the gland is provided with a filter membrane, and the filter membrane is attached to at least part of the diversion trench.

Drawings

In order to illustrate the technical solutions of the embodiments of the present invention more clearly, the drawings of the embodiments will be briefly described below, and it is obvious that the drawings in the following description only relate to some embodiments of the present invention, and are not intended to limit the present invention.

Fig. 1 is a structural diagram of a microfluidic chip according to an embodiment of the present invention;

fig. 2A is a schematic cross-sectional view of a microfluidic chip according to an example of an embodiment of the present invention;

fig. 2B is a schematic plan view of a flow guide groove of the microfluidic chip according to fig. 2A;

fig. 2C is a schematic cross-sectional view of a microfluidic chip according to another example of an embodiment of the present invention;

fig. 2D is a schematic cross-sectional view of a microfluidic chip in another example according to an embodiment of the present invention;

fig. 3A is a schematic plan view of a baffle slot according to another example of an embodiment of the present invention;

fig. 3B is a schematic plan view of a baffle slot according to another example of an embodiment of the present invention;

fig. 4A is a schematic view of a microfluidic chip assembly provided according to another embodiment of the present invention; and

fig. 4B is a schematic cross-sectional view of the microfluidic chip assembly shown in fig. 4A.

Detailed Description

In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined below to clearly and completely describe the technical solution of the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. All other embodiments, which can be obtained by a person skilled in the art without any inventive work based on the described embodiments of the present invention, belong to the protection scope of the present invention.

Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which the invention belongs. The use of "first," "second," and similar terms in the description herein do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items.

An embodiment of the utility model provides a micro-fluidic chip and micro-fluidic chip subassembly. The micro-fluidic chip comprises a sample introduction unit and a flow guide hole. The sample injection unit comprises a bottom plate and a side wall, and a cavity with an opening is formed by the bottom plate and the side wall in a surrounding mode; the diversion hole runs through the bottom plate. The bottom plate is including extending to the guiding gutter in guiding hole towards open-ended one side, and the degree of depth of guiding gutter is 0.1 ~ 2 millimeters, and the width of guiding gutter is 0.1 ~ 2 millimeters. The micro-fluidic chip adopts the guiding gutter that can form capillary force to play the continuous drainage effect to the sample in the introduction unit, make the sample collection easier, and the sample volume of collecting is great.

The following describes the microfluidic chip and the microfluidic chip assembly provided by the embodiments of the present invention with reference to the drawings.

Fig. 1 is a structural diagram of a microfluidic chip according to an embodiment of the present invention. As shown in fig. 1, the microfluidic chip includes a sample introduction unit 100 and a flow guide hole 200. The sample introduction unit 100 comprises a bottom plate 110, a side wall 120 connected to the bottom plate 110, and an opening 130 surrounded by the side wall 120, i.e. the sample introduction unit 100 is a non-closed cavity surrounded by the bottom plate 110 and the side wall 120. The guide holes 200 penetrate the bottom plate 110 in a direction perpendicular to the main plate surface of the bottom plate 110. As shown in fig. 1, the microfluidic chip further includes a flow guide groove 300, where the flow guide groove 300 is located on a side of the bottom plate 110 of the sample introduction unit 100 facing the opening 130 and extends to the flow guide hole 200, that is, the flow guide groove 300 is communicated with the flow guide hole 200. The depth of the guiding groove 300 is 0.1-2 mm, and the width of the guiding groove 300 is 0.1-2 mm to form a capillary force on the sample placed in the sample introduction unit 100, so as to accelerate the collection of the sample.

Fig. 1 schematically shows the number and shape of the channels 300, but the embodiment is not limited thereto, and the number of the channels in the embodiment of the present invention is at least one.

For the introduction of the sample unit that general bottom plate is only plane or conical surface, the utility model discloses a set up at least one and the small-size guiding gutter of water conservancy diversion hole intercommunication towards open-ended one side at the bottom plate of introduction of the sample unit, can utilize the capillary force that the guiding gutter formed to play the drainage effect that lasts to the sample in the introduction of the sample unit to make the sample collect more easily, the sample volume of collecting is great.

For example, as shown in fig. 1, the sample injection unit 100 of the microfluidic chip may have a cylindrical shape including an opening 130, the inner diameter of the sample injection unit 100 may be 5 to 40 mm, and the depth of the sample injection unit 100 may be 5 to 15 mm. For example, the inner diameter of the sample introduction unit 100 may be 15 to 30 mm, and the depth of the sample introduction unit 100 may be 5 to 10 mm. The volume of the sample introduction unit can be designed according to the sample requirement so that the sample introduction unit can accommodate the sample to be detected.

For example, the sample introduction unit 100 of the microfluidic chip is configured to accommodate a sample to be detected, for example, the sample may be a droplet or a fluid, etc.

For example, a filter membrane (the filter membrane 602 shown in fig. 4A) attached to at least a portion of the flow guide groove 300 may be disposed on a side of the flow guide groove 300 facing the opening 130 to filter the sample in the sample introduction unit 100, and the filtered sample may be automatically filtered to the flow guide hole by capillary force of the flow guide groove to achieve the purpose of rapid sample introduction, thereby simplifying the sample pretreatment process for a user.

For example, the guiding groove can be completely attached to the filtering membrane, so that the guiding groove can not only sufficiently and quickly collect the sample filtered by the filtering membrane by using capillary force, but also avoid bubbles generated in the process of collecting the sample. In addition, because the filtering membrane is attached to the flow guide groove, a gap is formed between the flow guide hole and the filtering membrane so as to prevent the flow guide hole from being blocked by the filtering membrane.

For example, the filtering membrane may be a blood filtering membrane to filter blood, and the blood serum filtered by the blood filtering membrane may be collected by the diversion trench and drained to the diversion hole.

In some examples, as shown in FIG. 1, the flow guide holes 200 have a circular cross-section taken parallel to the plane of the base plate 110, and have a diameter of 0.2-2 mm. From this, the water conservancy diversion hole can produce capillary force to the sample that is drained to the water conservancy diversion hole trompil by the guiding gutter, and then with sample drainage to the water conservancy diversion hole keep away from the open-ended tip of introduction unit in order to carry out subsequent detection. In order to ensure that the guiding holes can generate capillary force on the sample, the depth of the guiding grooves should not be too large, for example, 0.1-2 mm.

In some examples, as shown in fig. 1, the number of the guide channels 300 may be plural, the guide hole 200 is located at the center of the base plate 110, and the plurality of guide channels 300 surround the guide hole 200, so that more samples may be collected.

For example, as shown in fig. 1, the plurality of flow guide grooves 300 includes a plurality of first strip-shaped flow guide grooves 310, each of the first strip-shaped flow guide grooves 310 extends to the flow guide hole 200, i.e., each of the first strip-shaped flow guide grooves 310 communicates with the flow guide hole 200 to guide a sample collected by the first strip-shaped flow guide groove 310 using capillary force to the flow guide hole 200.

For example, as shown in fig. 1, the plurality of first linear guide grooves 310 have the same depth, and the plurality of first linear guide grooves 310 have the same width, so as to facilitate manufacturing. The term "main plate surface" refers to a portion of the surface of the bottom plate facing the opening, excluding the first linear guide groove and the position surrounded by the first linear guide groove, and this embodiment will be described by taking the main plate surface as a plane. Here, "depth" refers to a dimension of the first linear guide groove in a direction perpendicular to the main plate surface of the base plate, and "width" refers to a direction perpendicular to an extending direction of the first linear guide groove. The present embodiment is not limited to this, and the depth or the width of the plurality of first strip-shaped guiding grooves may also be different, and may be set according to actual requirements.

For example, each of the first linear guide grooves 310 may have a depth of 0.1 to 1 mm and a width of 0.1 to 1 mm.

In some examples, as shown in fig. 1, a plurality of first bar-shaped guide grooves 310 are radially distributed around the guide hole 200 and are all communicated with the guide hole 200. That is, the plurality of first strip-shaped guide grooves 310 radiate around the guide hole 200 so that the first strip-shaped guide grooves 310 are distributed at the center and the periphery of the bottom plate 110 of the sample injection unit 100, thereby facilitating the collection of samples at various positions of the bottom plate 110 and increasing the number of collected samples.

For example, as shown in fig. 1, the plurality of first strip-shaped guiding grooves 310 are uniformly distributed, which further facilitates the collection of samples at various positions of the bottom plate of the sample injection unit.

For example, as shown in fig. 1, the plurality of first guide grooves 310 do not communicate with each other, so that the sample collected by each first guide groove 310 can be more rapidly guided into the guide hole 200.

In some examples, as shown in fig. 1, a cross-section of the first linear guide channel 310 taken parallel to a plane of the base plate 110 is elongated.

In some examples, as shown in fig. 1, the end of the first linear guide groove 310 away from the guide hole 200 has a circular arc shape in section taken in parallel with the plane of the base plate 110, thereby facilitating the process.

For example, as shown in fig. 1, the length of the first linear guide grooves 310 may be 1.5 to 15 mm, i.e., each of the first linear guide grooves 310 may have a dimension in the extending direction of 1.5 to 15 mm. For example, the length of the first linear guide groove 310 may be 1.5 to 10 mm.

For example, as shown in fig. 1, the lengths of the first plurality of linear channels 310 are the same for ease of manufacturing.

Fig. 2A is a schematic cross-sectional view of a microfluidic chip in an example of the present embodiment. Fig. 2B is a schematic plan view of a flow guide groove of the microfluidic chip according to fig. 2A. As shown in fig. 1 and 2A-2B, first strip channels 310 may be grooves of equal depth, for example, 0.5 mm in depth.

In some examples, as shown in fig. 2A-2B, the two sidewalls 304 forming the first linear guide channel 310 extend beyond the edge of the guide hole 200, and the distance D between the end 302 of the sidewall 304 near the guide hole 200 and the edge of the guide hole 200 is no greater than 5 times the diameter of the guide hole 200. For the condition that the distance between the end part of the side wall close to the diversion hole and the edge of the diversion hole is larger, the distance between the end part of the side wall close to the diversion hole and the edge of the diversion hole is designed to be smaller, so that the capillary force generated by the diversion trench to the sample can drain all the samples to the diversion hole as much as possible, and a better diversion effect is achieved.

For example, the distance between the end 302 of the sidewall 304 near the flow guide hole 200 and the edge of the flow guide hole 200 is no greater than 2 times the diameter of the flow guide hole 200.

For example, as shown in fig. 2A-2B, the sidewalls 304 of two adjacent first linear channels 310 are connected near the end 302 of the pilot hole 200 to form a sharp corner 303, the sharp corner 303 is located beyond the edge of the pilot hole 200, and the distance D from the edge of the pilot hole 200 is no greater than 5 times the diameter of the pilot hole 200.

For example, fig. 2A-2B show that the end 302 of the sidewall 304 of the first linear guide channel 310 near the guide hole 200 is located at a suitable distance D beyond the edge of the guide hole 200, so that the sample flowing out of the first linear guide channel 310 can smoothly enter the guide hole 200, and part of the sample is prevented from being caught in the gap between the end 302 of the sidewall 304 and the guide hole 200.

Fig. 2C is a schematic cross-sectional view of a microfluidic chip in another example of the present embodiment. As shown in fig. 2C, the depth of the first bar groove 310 at a position close to the guide hole 200 is greater than the depth of an end thereof far from the guide hole 200, and the depth of the first bar groove 310 gradually changes along the extending direction of the first bar groove 310. That is, the depth of the sidewall 304 of the first strip guide 310 away from the first end 301 of the guide hole 200 is less than the depth of the sidewall 304 of the first strip guide 310 near the second end 302 of the guide hole 200, so that the bottom surface of the first strip guide 310 is formed as an inclined surface to facilitate the collection of a sample.

In the example, the surface of one side of the bottom plate of the sample introduction unit, which faces the opening, except for the guide groove and the position surrounded by the guide groove is a plane, for example, so that the filtering membrane can be attached to the guide groove more tightly, and the guide groove is favorable for collecting a sample filtered by the filtering membrane; and the bottom surface of the flow guide groove, which is far away from the opening side of the sample introduction unit, is designed into an inclined surface so as to facilitate collected samples to flow to the flow guide hole, and the collected sample amount is increased.

For example, the depth of the first linear guide channel 310 away from the first end 301 of the guide hole 200 may be 0.1 mm, and the depth of the first linear guide channel 310 near the second end 302 of the guide hole 200 may be 0.5 mm.

For example, the depth of the first linear guide channel 310 away from the first end 301 of the guide hole 200 may be 0.3 mm, and the depth of the first linear guide channel 310 near the second end 302 of the guide hole 200 may be 0.8 mm.

In some examples, as shown in fig. 2C, the two sidewalls 304 forming the first strip-shaped flow guide channel 310 extend to the edge of the flow guide hole 200, i.e., the end 302 of the sidewall 304 near the flow guide hole 200 is located at the edge of the flow guide hole 200. That is, the included angle 303 is located at the edge of the diversion hole 200 so that the sample flowing out of the first linear diversion trench directly enters the diversion hole, which is beneficial to sample collection.

For example, fig. 2D is a schematic cross-sectional view of a microfluidic chip in another example of the present embodiment. As shown in fig. 2D, the two sidewalls 304 forming the first strip-shaped flow guide groove 310 extend into the edge of the flow guide hole 200, i.e., the second end 302 of the sidewall 304 near the flow guide hole 200 extends into the edge of the flow guide hole 200. That is, the included angle 303 is located inside the edge of the diversion hole 200 so that the sample flowing out of the first linear diversion trench directly enters the diversion hole, which is beneficial to sample collection. The bottom surface of the first linear guide channel in this example may also be a slope as shown in fig. 2A to further promote the flow of the sample into the guide hole.

For example, as shown in fig. 2A to 2D, the number of the first strip-shaped guiding trenches 310 may be 3 to 30, but the present embodiment is not limited thereto, and the specific number of the guiding trenches may be increased or decreased according to the bottom plate area of the sample injection unit.

In some examples, as shown in fig. 2A-2D, in order to make the distance between the end 302 of the sidewall 304 forming the first bar-shaped channels 310 near the pilot hole 200 and the edge of the pilot hole 200 a suitable distance D, i.e., the distance between the cusp 303 and the edge of the pilot hole 200 a suitable distance D, the width of the first bar-shaped channels 310 may be 0.3 to 0.5 mm, and the number of the first bar-shaped channels 310 may be 15 to 18.

For example, the width of each first strip groove 310 may be 0.3 mm, and the number of first strip grooves 310 may be 18.

For example, the width of each first strip groove 310 may be 0.5 mm, and the number of first strip grooves 310 may be 15.

Fig. 3A is a schematic plan view of a baffle slot provided according to another example of the present embodiment. As shown in fig. 3A, the plurality of flow guide slots 300 further includes at least one ring of annular flow guide slots 320 surrounding the flow guide hole 200, and the annular flow guide slots 320 communicate with the first strip-shaped flow guide slots 310 to guide the sample collected by the annular flow guide slots 320 to the flow guide hole 200 through the first strip-shaped flow guide slots 310. When the flow guide slots 300 include the annular flow guide slots 320, the width of the flow guide slots 300 is equal to the annular width of the annular flow guide slots 320, and may be, for example, 0.1 to 2 mm, and the depth of the annular flow guide slots 320 may be 0.1 to 2 mm to generate capillary force on the filtered sample.

The first linear guide grooves in this example have the same features as the first linear guide grooves shown in fig. 2A to 2D, and are not described again here.

The present example schematically shows that the guide channels 300 include at least one ring of annular guide channels 320 and a plurality of first strip-shaped guide channels 310 radially distributed around the guide hole, but is not limited thereto. When the guiding gutter includes at least one circle of annular guiding gutter, the bar-shaped guiding gutter that the guiding gutter included as long as can communicate annular guiding gutter and guiding hole can, the distribution of bar-shaped guiding gutter does not do specific restriction, and its distribution can be not around the guiding hole and be radial distribution promptly, also can adopt other distributions.

In this example, by providing at least one ring of annular guide grooves, the contact area between the guide grooves and the sample can be increased to collect more samples without affecting the positional relationship between the first strip-shaped guide grooves and the guide holes.

Fig. 3A schematically shows one ring of annular guide grooves, but is not limited thereto, and may be two rings of annular guide grooves or more rings of annular guide grooves.

For example, as shown in fig. 3A, each first strip-shaped guide groove 310 is communicated with the annular guide groove 320 to perform better flow guiding effect on the sample.

For example, each of the first linear channels 310 shown in fig. 3A has the same length.

Fig. 3B is a schematic plan view of a baffle slot provided according to another example of the present embodiment. As shown in fig. 3B, the flow guide groove in this example is different from the example shown in fig. 3A only in that the flow guide groove further includes a plurality of second strip-shaped flow guide grooves 330 radially distributed around the flow guide hole 200, and the second strip-shaped flow guide grooves 330 have a length smaller than that of the first strip-shaped flow guide grooves 310 and communicate with the annular flow guide grooves 320.

For example, as shown in fig. 3B, each second strip-shaped guide groove 330 is located between two adjacent first strip-shaped guide grooves 310, and extends from a position where any one of the annular guide grooves 320 is far from the guide hole 200 to the annular guide groove 320 and communicates therewith.

For example, as shown in fig. 3B, the first strip-shaped guide grooves 310 are arranged along the circumferential direction of the guide hole 200 and extend from the side of the annular guide groove 320 away from the guide hole 200 to the guide hole 200, and the second strip-shaped guide grooves 330 are arranged along the circumferential direction of the guide hole 200 and extend from the side of the annular guide groove 320 away from the guide hole 200 to the annular guide groove 320.

For example, the plurality of second strip-shaped guide grooves 330 may extend to the same annular guide groove 320, or may extend to different annular guide grooves 320.

For example, the plurality of second strip-shaped guide grooves 330 communicate with the at least one annular guide groove 320, and each annular guide groove 320 is connected with the at least one first strip-shaped guide groove 310 to guide the sample collected in each guide groove 300 to the guide hole 200.

For example, as shown in fig. 3B, the first strip-shaped guide grooves 310 and the second strip-shaped guide grooves 330 are uniformly arranged along the circumferential direction of the guide hole 200 to increase the distribution density of the guide grooves in the peripheral region away from the guide hole 200, so that more samples can be collected.

For example, as shown in fig. 1, the length of the part of the microfluidic chip excluding the sample injection unit 100 may be 20 to 100 mm, and the width may be 10 to 50 mm.

In some examples, as shown in fig. 1, the microfluidic chip further comprises: a micro channel 400 located on one side of the bottom plate 110 of the sample injection unit 100 far away from the opening 130 and connected to the flow guide hole 200, and at least one reaction cell 500 connected to an end of the micro channel 400 far away from the flow guide hole 200. The micro flow channel 400 is used to communicate the flow guide hole 200 with the reaction cell 500 so that the filtered sample is guided to the reaction cell 500. The reaction cell 500 is used for storing a reaction reagent and providing a reaction site for a sample to be tested.

For example, the cross section of the reaction cell 500 taken in parallel with the plane of the bottom plate 110 of the sample injection unit 100 may be circular, square, or other shapes, and the depth of the reaction cell 500 may be 0.5-5 mm, which is not limited in this embodiment.

For example, the detection reagent is lyophilized or dried and then placed in the reaction cell 500 in advance, after the liquid sample enters the reaction cell 500 through the diversion trench 300, the diversion hole 200 and the micro flow channel 400, the reagent is rapidly re-melted and reacts, the detection light source irradiates from one side of the reaction cell 500, and the transmitted light signal is detected from the other side of the reaction cell 500.

For example, as shown in fig. 1, the number of the reaction cells 500 may be plural, and the micro flow channel 400 includes a plurality of branches to communicate with the plural reaction cells 500 in a one-to-one correspondence, so as to drain the sample in the flow guide hole 200 to each reaction cell. Different detection reagents can be placed in each reaction cell 500, so that multi-index detection of samples can be realized.

For example, as shown in fig. 1, each reaction cell 500 further includes an air vent hole 501 communicating with the outside to discharge air contained when the reaction cell 500 does not flow in the sample from the air vent hole 501 when the sample flows in the reaction cell 500.

For example, the microfluidic chip including the sample introduction unit, the flow guide hole, the flow guide groove, the micro flow channel, and the reaction cell may be formed by injection molding of a material having high light transmittance, such as Polycarbonate (PC), polymethyl methacrylate (PMMA), or glass.

The utility model provides a function that integrated sample of micro-fluidic chip filtered, advanced appearance and many indexes detected fast need not that the user of service filters or centrifugal treatment to blood and other liquid samples externally, has greatly simplified the manual operation flow, has reduced the consumption of sample and reagent to do not rely on large-scale instrument, greatly reduced use cost, make the on-the-spot instant detection possible.

Fig. 4A is a schematic view of a microfluidic chip assembly according to another embodiment of the present invention, and fig. 4B is a schematic cross-sectional view of the microfluidic chip assembly shown in fig. 4A. As shown in fig. 4A-4B, the microfluidic chip assembly includes the microfluidic chip 1000 shown in fig. 1-3B, the microfluidic chip assembly further includes a filter membrane 602 attached to at least a portion of the flow guide groove 300, and a cover 601 covering the opening 130 of the sample introduction unit 100, and at least a portion of the material of the cover 601 facing the bottom plate 110 of the sample introduction unit 100 is an elastic material. The gland 601 is used with the sample introduction unit 100 to form a sealed cavity, so that the risk of sample contamination can be reduced.

For example, the material of the gland 601 may include an elastic material such as Polycarbonate (PC) or polymethyl methacrylate (PMMA).

In this embodiment, the elastic material of the pressing cover deforms when pressure is applied, for example, the pressing cover is recessed into the sample injection unit, so that pressure can be applied to the sealed cavity (that is, the downward pressure of the sample is increased by the pressing seal between the pressing cover and the sample injection unit) to enable the sample to rapidly pass through the filtering membrane and enter the microchannel, and then enter the reaction cell. From this, for test paper strip normal pressure filter blood membrane filtration technique, the utility model discloses a gland lid that has elastic material closes behind the appearance unit, through exerting pressure to the gland, can shorten the time of filtering the sample, improve the filter effect, and whole micro-fluidic chip subassembly simple structure, easy operation.

When the gland in this embodiment applies pressure to the sample in the sample introduction unit through deformation, the collection of the pressurized sample can be facilitated because the flow guide hole is positioned in the center of the bottom plate of the sample introduction unit.

The distance between the gland and the opening of the sample injection unit can be adjusted according to actual needs, the compression volume meeting the requirements is achieved, and when pressure is applied to the gland, the pressure in the cavity of the sample injection unit is increased, so that a sample can enter the micro-channel through the filtering membrane quickly.

For example, as shown in fig. 4A and 4B, the filter membrane 602 may be attached to at least a portion of the flow leader 300 by an annular double-sided adhesive 603. The annular double-sided adhesive tape 603 can be located at the edge of the bottom plate of the sample injection unit 100 and does not coincide with the diversion trench 300, so that the filter membrane can be well attached to the diversion trench, and the diversion trench can collect the sample filtered by the filter membrane to the maximum extent. Certainly, the shape of the double-sided adhesive tape for attaching the filter membrane to the flow guide groove is not limited in this embodiment, and can be designed according to requirements; the double faced adhesive tape and the diversion trench are not limited to be misaligned, and the diversion trench is not influenced to collect a sample filtered by the filtering membrane.

For example, as shown in fig. 1 and fig. 4A to fig. 4B, when the microfluidic chip 1000 is formed by injection molding, a side of the micro channel 400 of the microfluidic chip 1000, which is away from the gland 601, is a grooved structure, and a transparent bottom cover plate 605 needs to be attached to achieve sealing. Thus, the microfluidic chip assembly further includes a transparent bottom cover plate 605 attached to the side of the microchannel 400, the flow guiding holes 200, and the reaction cell 500 away from the cover 601, for example, the transparent bottom cover plate 605 may be attached to the microfluidic chip 1000 by a sealing double-sided adhesive tape. The embodiment is not limited thereto, and the transparent bottom cover plate may also be attached to the microfluidic chip by laser welding or the like to seal the micro flow channel.

For example, as shown in fig. 4A-4B, the microfluidic chip assembly further comprises a gas permeable membrane 604 attached to the vent hole 501, the gas permeable membrane 604 being permeable to air but impermeable to the sample to prevent the sample from overflowing the reaction cell.

The microfluidic chip assembly provided by the embodiment can have the function of realizing biochemical detection. The microfluidic chip assembly provided in this embodiment is also applicable to other detection methods using liquid samples for analysis, such as immunoassay.

The utility model provides a function that integrated sample of micro-fluidic chip subassembly filtered, advances appearance and many indexes detection fast need not that the user of service externally filters or centrifugal treatment blood and other liquid samples, has greatly simplified the manual operation flow, has reduced the consumption of sample and reagent to do not rely on large-scale instrument, greatly reduced use cost, make the on-the-spot instant detection possible.

The following points need to be explained:

(1) in the drawings of the embodiments of the present invention, only the structures related to the embodiments of the present invention are referred to, and other structures may refer to general designs.

(2) Features of the present invention may be combined with each other in the same embodiment and in different embodiments without conflict.

The above description is only an exemplary embodiment of the present invention, and is not intended to limit the scope of the present invention, which is defined by the appended claims.

Claims (17)

1. A microfluidic chip, comprising:

the sample injection unit comprises a bottom plate and a side wall, wherein a cavity with an opening is formed by the bottom plate and the side wall in a surrounding mode;

the flow guide hole penetrates through the bottom plate of the sample injection unit along the direction vertical to the main plate surface of the bottom plate;

one side of the bottom plate facing the opening comprises a guide groove extending to the guide hole, the depth of the guide groove is 0.1-2 mm, and the width of the guide groove is 0.1-2 mm.

2. The microfluidic chip according to claim 1, wherein the number of the flow guide grooves is plural, the flow guide hole is located at the center of the bottom plate, and the plural flow guide grooves surround the flow guide hole.

3. The microfluidic chip according to claim 2, wherein the plurality of flow guide grooves comprises a plurality of first bar-shaped flow guide grooves radially distributed around the flow guide hole, each of the first bar-shaped flow guide grooves extending to the flow guide hole.

4. The microfluidic chip according to claim 3, wherein the first bar-shaped channel has a depth at a position close to the flow guide hole greater than a depth at an end portion away from the flow guide hole, and the depth of the first bar-shaped channel gradually changes along an extending direction of the first bar-shaped channel.

5. The microfluidic chip according to claim 3, wherein two sidewalls forming the first linear channel extend into edges of the flow guide hole.

6. The microfluidic chip according to claim 3, wherein two sidewalls forming the first linear channel extend to edges of the flow guide hole.

7. The microfluidic chip according to claim 3, wherein the flow guide holes have a circular cross section taken on a plane parallel to the main plate surface of the bottom plate, and have a diameter of 0.2-2 mm.

8. The microfluidic chip according to claim 7, wherein two sidewalls forming the first linear channel extend beyond the edge of the flow guide hole, and a distance between an end of the sidewall near the flow guide hole and the edge of the flow guide hole is not greater than 5 times a diameter of the flow guide hole.

9. The microfluidic chip according to any of claims 3 to 8, wherein the width of the first channel is 0.3 to 0.5 mm, and the number of the first channels is 15 to 18.

10. The microfluidic chip according to claim 9, wherein the first channel has a length of 1.5-15 mm.

11. The microfluidic chip according to any of claims 3 to 8, wherein the end of the first strip-shaped channel away from the flow guide hole has a circular arc shape in cross section taken on a plane parallel to the bottom plate.

12. The microfluidic chip according to claim 3, wherein the plurality of flow guide grooves further comprises at least one ring of annular flow guide grooves surrounding the flow guide hole, and the at least one ring of annular flow guide grooves is communicated with the first linear flow guide groove.

13. The microfluidic chip according to claim 12, wherein the plurality of channels further comprises a plurality of second strip-shaped channels radially distributed around the channel hole, the second strip-shaped channels having a length less than that of the first strip-shaped channels and communicating with the at least one ring of annular channels.

14. The microfluidic chip according to claim 13, wherein each of the second strip-shaped channels is located between two adjacent first strip-shaped channels, and extends from a position of any one of the at least one ring of annular channels away from the flow guide hole to the annular channel and is communicated with the annular channel.

15. The microfluidic chip according to any of claims 1 to 8, further comprising:

the micro-channel is positioned on one side of the bottom plate of the sample injection unit, which is far away from the opening, and is communicated with the flow guide hole; and

and the at least one reaction tank is connected with the end part of the micro-channel far away from the diversion hole.

16. A microfluidic chip assembly comprising the microfluidic chip of any one of claims 1-15 and a cover covering the opening of the sample introduction unit, wherein at least a portion of the material of the cover facing the bottom plate of the sample introduction unit is an elastic material.

17. The microfluidic chip assembly according to claim 16, wherein a side of the flow guide groove facing the capping is provided with a filter membrane, and the filter membrane is attached to at least a portion of the flow guide groove.

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