CN115322926A - Rhodococcus pyridinivorans and application thereof in repairing continuous cropping and continuous cropping soil - Google Patents
Rhodococcus pyridinivorans and application thereof in repairing continuous cropping and continuous cropping soil Download PDFInfo
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- CN115322926A CN115322926A CN202210895512.3A CN202210895512A CN115322926A CN 115322926 A CN115322926 A CN 115322926A CN 202210895512 A CN202210895512 A CN 202210895512A CN 115322926 A CN115322926 A CN 115322926A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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Abstract
The invention discloses Rhodococcus pyridinivorans GXUN1036. The strain is preserved in China general microbiological culture Collection center (CGMCC) at 6 months and 2 days in 2022, and the biological preservation number is as follows: CGMCC NO.25002. The strain can degrade various phenolic acid compounds, degrade 2000mg/L p-hydroxybenzoic acid contained in an inorganic salt culture medium by 99.7% within three days, degrade vanillic acid, gallic acid and ferulic acid, relieve continuous cropping obstacles caused by the phenolic acid compounds, and has good repairing effect on tomatoes planted in continuous cropping soil. Meanwhile, the compound microbial organic fertilizer has a bioremediation effect on continuous cropping obstacles caused by the phenolic acid substances, can be prepared into a microbial agent, a compound microbial fertilizer and a biological organic fertilizer to restore continuous cropping soil, and has good economic value and application prospect.
Description
Technical Field
The invention relates to the technical field of agricultural microorganisms and ecological restoration, in particular to Rhodococcus pyridinivorans and application thereof.
Background
The continuous cropping obstacle problem seriously influences the production and sustainable development of crops, autotoxic substances generated by plants are main factors causing the continuous cropping obstacle of the crops, and the autotoxic substances can enter soil through plant leaching, root secretion, plant residues and other ways to influence the growth of next-stubble crops. Autotoxic substances secreted by plant roots, such as phenolic acids like p-hydroxybenzoic acid, are common autotoxic substances. Researches show that the phenolic acid provides abundant nutrient substances for pathogenic fungi, and greatly reduces the fertility of soil and the yield and quality of crops.
The phenolic acid substances can be degraded in the environment through two routes, namely non-biological and biological, wherein the former route comprises photolysis and hydrolysis, and the latter route mainly comprises microbial degradation, and the rate of photolysis degradation is far lower than that of biological degradation in most cases, so that the microbial degradation is the main degradation route of the phenolic acid substances in the natural environment.
Research shows that the rhodococcus can degrade various natural organic compounds such as aliphatic, aromatic, heterocyclic, polycyclic aromatic, alicyclic hydrocarbon, nitrile, cholesterol and the like, so the rhodococcus is a potential environment treatment specific bacterium and has good application prospect in the aspects of repairing continuous cropping soil and treating industrial wastewater, marine oil pollution and the like in environmental pollution.
Tomatoes are common economic crops for cultivation, and continuous cropping obstacles become main limiting factors influencing the yield and quality of the tomatoes with the increase of planting years. The phenolic acid substances are autotoxic substances produced by the tomatoes, comprise vanillic acid, p-hydroxybenzoic acid, ferulic acid and the like, and have a remarkable inhibiting effect on the growth of the tomatoes. Biological measures are adopted to promote the degradation of phenolic acid substances in the soil, and the continuous cropping obstacles can be effectively reduced.
In recent years, measures for repairing continuous cropping and continuous cropping soil by microorganisms have attracted attention. Bacterial manure is one of common soil remediation products, is a preparation for remedying and improving soil fertility through growth and metabolic activities of microorganisms and interacting with plants to guarantee plant growth, and is widely used in agriculture.
Disclosure of Invention
In view of the prior art, the invention provides a strain of Rhodococcus pyridinivorans GXUN1036 for degrading phenolic acids. The Rhodococcus pyridinii GXUN1036 is separated from mangrove rhizosphere soil, can degrade various phenolic acid substances such as p-hydroxybenzoic acid, vanillic acid, gallic acid, ferulic acid and the like, and can repair phenolic acid soil generated by continuous cropping and continuous cropping.
The invention provides a microbial inoculum containing the Rhodococcus pyridinii GXUN1036.
The invention provides application of the Rhodococcus pyridinii GXUN1036 in repairing continuous cropping obstacles of phenolic acids, wherein the Rhodococcus pyridinii GXUN1036 can be used for microbial agents, compound microbial fertilizers and biological organic fertilizers.
Specifically, the invention relates to the following technical scheme:
the invention separates a bacterial strain capable of degrading p-hydroxybenzoic acid from the rhizosphere soil of mangrove forest in the north sea. The strain can degrade p-hydroxybenzoic acid, vanillic acid, gallic acid and ferulic acid at a concentration of 2000mg/L in inorganic salt ion culture medium.
The invention provides Rhodococcus pyridinivorans (Rhodococcus pyridinivorans) GXUN1036, which has been deposited in China general microbiological culture Collection center (CGMCC for short, with the address of No. 3 Xilu 1. In North Chen of the south-facing area in Beijing) on 2.6.2022 days, and the biological preservation number is: CGMCC NO.25002.
The colony and thallus characteristics of the Rhodococcus pyridinii GXUN1036 strain are as follows:
the lawn growing on the LB plate is raised and pink, the colony is not transparent, the surface is smooth, the colony is slightly glossy, the edge is regular, and the lawn can grow on an inorganic salt solid culture medium taking p-hydroxybenzoic acid as a unique carbon source; scanning electron microscope results show that the thallus is rod-shaped, convex and has no flagellum, the length of the thallus is 300-781 nm, and the width of the thallus is 3-5 nm.
The LB culture medium comprises the following components: yeast extract 5g tryptone 10g sodium chloride 10g agar 20g distilled water 1000mL, pH 7.0.
The bacterial fertilizer containing the Rhodococcus pyridinii GXUN1036 comprises a microbial agent, a compound microbial fertilizer and a biological organic fertilizer, and also belongs to the protection scope of the invention.
The bacterial fertilizer comprises Rhodococcus pyridinii GXUN1036, other functional strains, carriers, fertilizers and organic fertilizers;
the bacterial manure can contain auxiliary materials such as humic acid, biochar, animal excrement, straws of various crops, straws, peanut skin and the like besides the Rhodococcus pyridinii GXUN1036. The microbial inoculum can also comprise a carrier, and the carrier can be a solid carrier or a liquid carrier. The solid support may be selected from mineral materials, plant materials or polymeric compounds; the mineral material may be one or more of clay, talc, kaolin, zeolite and diatomaceous earth; the plant material may be at least one of corn flour, bean flour or starch; the polymer compound may be polyvinyl alcohol. The liquid carrier can be an organic solvent, vegetable oil or water;
the bacterial fertilizer not only contains Rhodococcus pyridinii GXUN1036, but also can comprise chemical fertilizers, such as elemental fertilizers and compound fertilizers, such as nitrogen fertilizers, phosphate fertilizers, potassium fertilizers and the like;
the bacterial fertilizer not only contains Rhodococcus pyridinii GXUN1036, but also can comprise organic fertilizers consisting of various animals, plant residues and metabolites, such as human and animal wastes, straws, animal residues and slaughterhouse wastes;
in the bacterial manure, the Rhodococcus pyridinivorans GXUN1036 can be in the form of cultured living cells, fermentation broth of the living cells, filtrate of cell culture or mixture of cells and filtrate.
The state of the bacterial manure can be liquid, emulsion, suspending agent, granule, powder, wettable powder or water dispersant.
Further, the invention also provides a preparation method of the Rhodococcus pyridinii GXUN1036 viable bacteria agent, which comprises the following steps:
inoculating Rhodococcus pyridinii GXUN1036 into a fermentation culture medium, and culturing for 72h at 30-37 ℃ to obtain Rhodococcus pyridinii GXUN1036 fermentation liquor;
diluting the Rhodococcus pyridinii fermentation liquor to 5-10% by using sterile water to obtain a viable bacteria agent;
preferably, the viable bacteria count of the Rhodococcus pyridinii strain GXUN1036 viable bacteria agent is 2-3 × 10 9 cfu/mL。
Preferably, the formula of the fermentation medium is 5g of yeast extract, 10g of tryptone, 10g of sodium chloride and 10g of distilled water, 1000mL, and the pH value is 7.0.
The use method of the prepared viable bacteria preparation of the Rhodococcus pyridinii GXUN1036 strain comprises the following steps:
the Rhodococcus pyridinii GXUN1036 strain viable bacteria microbial inoculum is diluted to 5-10% by adding sterile water, and roots are dipped or irrigated to plant rhizosphere during seedling transplanting.
The application of the rhodococcus pyridinivorus GXUN1036 or a microbial inoculum containing the rhodococcus pyridinivorus GXUN1036 in any one of the following 1-4 also belongs to the protection scope of the invention:
1. the application of promoting the growth of plants in phenolic acid environment;
2. the application in preparing bacterial manure for phenolic acid soil remediation;
3. the application of the phenolic acid in degrading soil;
4. can be used for degrading the autotoxic substances generated by crops and lightening continuous cropping obstacles.
In the above application, the phenolic acid comprises: p-hydroxybenzoic acid, vanillic acid, gallic acid and ferulic acid.
In the above applications, the autotoxic substances produced by the crops include: p-hydroxybenzoic acid, vanillic acid, gallic acid and ferulic acid.
The invention has the beneficial effects that:
the Rhodococcus pyridinii GXUN1036 can degrade plant autotoxic substances such as p-hydroxybenzoic acid, vanillic acid, gallic acid, ferulic acid and the like, after the Rhodococcus pyridinii GXUN1036 is cultured for 3 days, the degradation rates of the p-hydroxybenzoic acid, the vanillic acid, the gallic acid and the ferulic acid are respectively 99.7%, 98.8%, 90.1% and 15.4%, and the viable bacteria preparation can be used for degrading autotoxic substances generated by the phenolic acid soil tomatoes, so that continuous cropping obstacles are reduced, the growth of the tomatoes is promoted, and the yield of the tomatoes is increased.
Drawings
FIG. 1 is a scanning electron micrograph (c) of Rhodococcus pyridinii grown in example 1 on LB (a) and p-hydroxybenzoic acid as sole carbon sources (b);
FIG. 2 is a contiguous tree of the strain constructed based on the 16S rRNA gene sequence of example 1;
FIG. 3 shows the growth and degradation of p-hydroxybenzoic acid by Rhodococcus pyridinivorans in example 2;
FIG. 4 shows the degradation of vanillic acid, gallic acid and ferulic acid by Rhodococcus pyridinii in example 2;
FIG. 5 is the stem height and stem thickness values for the tomato growth of the tomato experiment in example 3;
FIG. 6 is the values of fresh and dry weight for the growth of the tomato potting experiment of example 3.
Detailed Description
The invention is further described with reference to the following figures and specific embodiments. The following examples are preferred embodiments of the present invention, but are not intended to limit the scope of the present invention in any manner. The invention mainly describes the strains and application ideas based on the strains, and the simple parameter replacement in the embodiment can not be repeated in the embodiment, but the invention is not limited thereby, and any other changes, modifications, substitutions, combinations and simplifications which do not depart from the spirit and principle of the invention should be regarded as equivalent replacement ways which are included in the scope of the invention. It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
The inorganic salt culture medium used in the invention is as follows: k 2 HPO 4 0.05g、MgSO 4 0.02g、CaCl 2 0.01g、FeCl 3 0.001g、MnSO 4 0.001g、NH4NO 3 0.1g、KH 2 NO 3 0.05g and NaCl 0.02g, the pH was adjusted to 7.0, and 2g of p-hydroxybenzoic acid and 1000mL of distilled water were added.
The nutrient medium is as follows: yeast extract 5g tryptone 10g sodium chloride 10g distilled water 1000mL, pH 7.0.
The plate solid culture medium is corresponding culture medium added with 1.5 percent agar.
Example 1 isolation and characterization of Rhodococcus pyridinii GXUN1036
1. Screening and isolation of strains Using P-hydroxybenzoic acid
Weighing 2g of mangrove sediments, placing the mangrove sediments in a 10mL centrifuge tube, adding 2mL sterile water and a glass strain (sterilization) shaker, shaking for 10min, and preparing into suspension. Ten times of dilution method is adopted to respectively dilute the suspension into 10 -3 、10 -4 、10 -5 The diluted solution of (1) is prepared by respectively taking 100 mu L of diluted suspension, coating the diluted suspension on an inorganic salt culture medium containing p-hydroxybenzoic acid, culturing for 7d in a constant temperature box at 30 ℃ in a dark place, observing and selecting strains capable of growing on a solid culture medium taking the p-hydroxybenzoic acid as a unique carbon source, purifying for multiple times by a three-zone streak separation method until a single colony is separated from the culture medium, and separating a strain for degrading the p-hydroxybenzoic acid, wherein the strain is named as GXUN1036.
2. Morphological observation and identification of the Strain GXUN1036
(1) The method comprises the following steps: and (3) placing the activated strain on an LB plate and a solid inorganic salt plate containing 2000mg/L p-hydroxybenzoic acid, culturing for 3d at constant temperature and in dark place at 30 ℃, observing the growth condition of the strain, and observing the cell morphology of the bacteria by using a scanning electron microscope. Extracting genome DNA of the strain GXUN1036 by adopting a bacterial genome extraction kit, amplifying 16S rRNA genes of the strain GXUN1036 by PCR, wherein the sequence of an amplified upstream primer is as follows: 27F (5: 1492R (5 'GGTTACCTTGTT ACGACTT-3'), 25. Mu.L of total reaction system, 12.5. Mu.L of PCR premix (2 XTaq Master Mix), and ultrapure water (ddH) 2 O) 10.5. Mu.L, 1. Mu.L of each of the upstream and downstream primers, and 1. Mu.L of the amplification template. The set reaction program was: pre-denaturation at 94 ℃ for 5min, denaturation at 95 ℃ for 10s, annealing at 55 ℃ for 20s, and extension at 72 ℃ for 1.5min, with 32 cycles in total, and extension at 72 ℃ for 10min. The PCR product was detected by agarose (1%) gel electrophoresis, and the amplification product corresponding to the expected target band size was selected by M arker band and sent to Biotechnology engineering (Shanghai) Ltd for sequencing. In Ezbiocloud: (http：//eztaxon-e.ezbiocloud.net)16S rRNA gene sequence of GXUN1036 and the gene sequence thereof on websiteThe standard strain is subjected to comparison analysis to obtain 16S rRNA of a strain similar to GXUN1036, a phylogenetic tree is constructed by using a proximity method (Neighbor-join) in MEGA6 software, and 1000 times of repeated verification is carried out by using a self-expanding method (Bootstrap).
(2) As a result:
as shown in FIG. 1, the growth of Rhodococcus pyridinii on LB (a) and P-hydroxybenzoic acid as sole carbon sources medium (b), scanning electron micrograph (c);
as shown in fig. 2, a contiguous tree of strains constructed based on the 16S rRNA gene sequence;
as shown in the above figure, the strain GXUN1036 has 100% identity with Rhodococcus pyritivorans.
Example 2 degradation Performance test of Rhodococcus pyridinii GXUN1036 Strain
(1) The method comprises the following steps: 4 phenolic acids of p-hydroxybenzoic acid, vanillic acid, gallic acid and ferulic acid are selected as degradation target products, and the capability of GXNU1036 in degrading the phenolic acids is measured. Adding the 4 phenolic acids into 20mL of inorganic salt liquid culture medium respectively to make the final concentration of the phenolic acid in the culture medium be 2g/L, inoculating 200 μ L of bacterial suspension with OD600 value of 0.6 into the culture medium, and performing shake culture at 30 deg.C and 200rmp for 72h. Freezing the fermentation liquid in a refrigerator at-80 deg.C for 3h, and freeze-drying with a freeze-drying machine for 72h. Weighing the lyophilized product, adding ten times of methanol, performing ultrasonic treatment for 10min, filtering with 0.45 μm organic filter membrane to extract product, and measuring residual concentrations of p-hydroxybenzoic acid, vanillic acid, gallic acid, and ferulic acid at 254nm, 290nm, 311nm, and 270nm of ultraviolet spectrophotometer. The degradation rate was calculated using a standard curve for each phenolic acid. Each experimental group was set up in 3 replicates and a sample of uninoculated broth was used as a blank.
(2) As a result:
as shown in fig. 3, growth and degradation of p-hydroxybenzoic acid by rhodococcus pyridinatus;
as shown in the above figure, the average degradation rate of the Rhodococcus pyridinii on hydroxyl groups is more than 99%.
As shown in fig. 4, the rhodococcus pyridinii degrades vanillic acid, gallic acid, ferulic acid;
as shown in the above figure, the degradation rates of vanillic acid, gallic acid and ferulic acid are respectively 98.8%, 90.1% and 15.4%.
EXAMPLE 3 preparation of a viable bacterial preparation of Rhodococcus pyridinii GXUN1036 Strain
(1) Activating strains: the Rhodococcus pyridinii GXUN1036 is transferred to an LB medium plate and cultured for 72h at 30 ℃ for activation.
(2) Preparing fermentation liquor: the activated Rhodococcus pyridinii GXUN1036 lawn is scraped by an inoculating loop and inoculated in 200mL LB liquid culture medium, and cultured for 72h at 30 ℃.
(3) Preparation of a strain viable bacteria agent: diluting the fermentation liquor by 5-10%, and the viable count of the viable bacteria agent of Rhodococcus pyridinatus GXUN1036 is 2-3 × 10 9 cfu/mL。
Example 4 Redox Rhodococcus pyridinii GXUN1036 inoculum for the remediation of tomato growth in hydroxybenzoic acid soil
(1) The method comprises the following steps: the control group contained nutrient soil in a flowerpot, and the experimental group contained p-hydroxybenzoic acid in the nutrient soil so that the final concentration of p-hydroxybenzoic acid was 10g/kg and then in the flowerpot. The test adopts a root irrigation treatment method, and seedlings growing in a seedling tray are transferred to a flowerpot filled with nutrient soil. The following 5 treatments were tested: t1, irrigating 10% sterile water with 70g of normal nutrient soil; t2, 70g, 10g/kg of p-hydroxybenzoic acid nutrient soil is irrigated with 10% Rhodococcus pyridinii GXUN1036 microbial inoculum; t3, 70g, of 10g/kg of p-hydroxybenzoic acid nutrient soil is used for irrigating 5% Rhodococcus pyridinii GXUN1036 microbial inoculum; t4, 70g of normal nutrient soil is irrigated with 10% Rhodococcus pyridinii GXUN1036 microbial inoculum. T5, irrigating 10% sterile water with 70g of normal nutrient soil. The test is carried out in a greenhouse with a temperature of 24-28 ℃, an RH of 75-90% and a photoperiod of 12h. Each treatment was 3 strains, and the experiment was repeated 3 times. Fresh weight, dry weight, plant height and stem thickness of the tomatoes were measured 28d after inoculation.
(2) Results
As shown in FIG. 5, the 10g/kg p-hydroxybenzoic acid soil irrigation 10% Rhodococcus pyridinii GXUN1036 microbial inoculum, the 10g/kg p-hydroxybenzoic acid soil irrigation 5% Rhodococcus pyridinii GXUN1036 microbial inoculum, the normal soil irrigation 10% Rhodococcus pyridinii GXUN1036 microbial inoculum, and the normal soil irrigation 10% Rhodococcus pyridinii GXUN1036 microbial inoculum have significant differences in plant height and stem thickness from the 10g/kg p-hydroxybenzoic acid soil irrigation 10%, indicating that the Rhodococcus pyridinii GXUN1036 microbial inoculum can recover tomatoes stressed by the p-hydroxybenzoic acid soil.
As shown in FIG. 6, the fresh weight and the dry weight of 10g/kg of P-hydroxybenzoic acid soil irrigated with 10% Rhodococcus pyridinii GXUN1036 microbial inoculum, 10g/kg of P-hydroxybenzoic acid soil irrigated with 5% Rhodococcus pyridinii GXUN1036 microbial inoculum, normal soil irrigated with 10% Rhodococcus pyridinii GXUN1036 microbial inoculum, and normal soil irrigated with 10% Rhodococcus pyridinii GXUN1036 microbial inoculum are significantly different from 10g/kg of P-hydroxybenzoic acid soil irrigated with 10% Rhodococcus pyridinii GXUN1036 microbial inoculum, which indicates that the P-hydroxybenzoic acid soil stressed tomatoes can be recovered by the Rhodococcus pyridinii GXUN1036 microbial inoculum.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, it is intended that all such modifications and alterations be included within the scope of this invention as defined in the appended claims.
Claims (7)
1. Rhodococcus pyridinivorans GXUN1036 with biological deposit number: CGMCC NO.25002.
2. A microbial agent, a compound microbial fertilizer and a biological organic fertilizer containing the Rhodococcus pyridinivorans strain GXUN1036 according to claim 1.
3. The method according to claim 2, wherein the microbial agent comprises Rhodococcus pyridinii strain GXUN1036 and other functional strains and vectors.
4. The compound microbial fertilizer according to claim 2, wherein the compound microbial fertilizer comprises Rhodococcus pyridinii GXUN1036 and other functional strains and nutrients.
5. The biological organic fertilizer as claimed in claim 2, wherein the biological organic fertilizer comprises Rhodococcus pyridinivorans GXUN1036 and other functional strains and organic fertilizers.
6. The microbial agent, the compound microbial fertilizer and the bio-organic fertilizer according to claim 2, wherein the state of the microbial agent, the compound microbial fertilizer and the bio-organic fertilizer can be liquid, emulsion, suspension, granule, powder, wettable powder or water dispersion.
7. The microbial agent, the compound microbial fertilizer and the bio-organic fertilizer as claimed in any one of claims 3 to 5, which is at least one of the following (b 1) to (b 4):
(b1) Relieving the damage of soil phenolic acid stress to plants, wherein the phenolic acid substances comprise p-hydroxybenzoic acid, vanillic acid, gallic acid and ferulic acid;
(b2) Improving the capability of the plant to resist the stress of soil phenolic acid, wherein the phenolic acid substances comprise p-hydroxybenzoic acid, vanillic acid, gallic acid and ferulic acid;
(b3) The microbial agent, the compound microbial fertilizer and the biological organic fertilizer are used as the microbial agent, the compound microbial fertilizer and the biological organic fertilizer of phenolic acid soil plants;
(b4) Improve the physical and chemical properties of the continuous cropping phenolic acid soil.
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| CN108546658A (en) * | 2018-04-10 | 2018-09-18 | 暨南大学 | One plant of phosphorus-solubilizing bacteria and its compound microbial inoculum and application with DEHP degradation bacterias |
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| RU2803633C1 (en) * | 2023-05-22 | 2023-09-18 | Федеральное государственное автономное образовательное учреждение высшего образования "Пермский государственный национальный исследовательский университет" | Method for biodegradation of hydroxylated benzoic acids using rhodococcus ruber p25 strain |
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