CN115322899A - Cell culture device for cell biology experiments - Google Patents
Cell culture device for cell biology experiments Download PDFInfo
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- CN115322899A CN115322899A CN202210636810.0A CN202210636810A CN115322899A CN 115322899 A CN115322899 A CN 115322899A CN 202210636810 A CN202210636810 A CN 202210636810A CN 115322899 A CN115322899 A CN 115322899A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 26
- 238000002474 experimental method Methods 0.000 title claims abstract description 10
- 238000011084 recovery Methods 0.000 claims abstract description 12
- 230000017105 transposition Effects 0.000 claims description 62
- 239000007788 liquid Substances 0.000 claims description 37
- 238000007789 sealing Methods 0.000 claims description 32
- 230000001360 synchronised effect Effects 0.000 claims description 30
- 230000007704 transition Effects 0.000 claims description 17
- 239000002516 radical scavenger Substances 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims 1
- 238000009423 ventilation Methods 0.000 abstract description 65
- 230000008859 change Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 5
- 238000010367 cloning Methods 0.000 description 4
- 238000005213 imbibition Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention discloses a cell culture device for cell biology experiments, which relates to the technical field of cell culture equipment and comprises an incubator shell, wherein an incubator sliding door and a ventilation assembly are arranged on the incubator shell, a culture cavity is arranged in the incubator shell and is positioned on the lower side of the incubator sliding door, an input cylinder is arranged on the incubator shell, an input control assembly is arranged at the top end of the input cylinder, the bottom end of the input cylinder is connected with a recovery box, the recovery box is arranged on the lower side of the incubator shell, an input assembly is arranged in the input cylinder, a suction assembly is arranged in the incubator shell, the input end of the suction assembly is arranged in the input cylinder, a feeding port is arranged on the culture cavity, the output end of the suction assembly is arranged on the upper side of the feeding port, and a culture control assembly is arranged on the culture cavity. The culture control assembly provided by the invention can enable the placing table to extend out while opening the sliding door, and can automatically change the position when the placing table is retracted.
Description
Technical Field
The invention relates to the technical field of cell culture equipment, in particular to a cell culture device for cell biology experiments.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process, both for the whole bioengineering technique and for one of the techniques of biological cloning, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
At present, the cell culture in a laboratory needs manual operation from preparation to culture by a laboratory, and the cell culture in a cell biology experiment usually needs a plurality of groups of culture, so that the workload of the laboratory is greatly increased, and a cell culture device for the cell biology experiment is needed.
In the prior art, a chinese patent publication (publication) No. CN108384719a discloses a cell co-culture apparatus, which adopts the following technical scheme: including cell coculture unit, cell coculture unit has the culture dish of two at least range upon range of settings, is equipped with the interior circulation channel or the interior infiltration passageway of both inner chambers of intercommunication between two adjacent culture dishes, is connected with the outer circulation pipeline that supplies the nutrient solution to flow on at least one of them culture dish, is equipped with the pumping device that drives the nutrient solution in each culture dish and flows on the outer circulation pipeline. This device can simulate the environment in the body, but cannot realize automated culture of cells.
Disclosure of Invention
In order to solve the technical problems, the invention provides a cell culture device for cell biology experiments, which comprises an incubator shell, wherein the incubator shell is provided with an incubator sliding door and a ventilation assembly, a culture chamber is arranged in the incubator shell and is positioned on the lower side of the incubator sliding door, the incubator shell is provided with an input cylinder, the top end of the input cylinder is provided with an input control assembly, the bottom end of the input cylinder is connected with a recovery box, the recovery box is arranged on the lower side of the incubator shell, the input cylinder is internally provided with an input assembly, the incubator shell is internally provided with a suction assembly, the input end of the suction assembly is arranged in the input cylinder, the culture chamber is provided with a feed inlet, the output end of the suction assembly is arranged on the upper side of the feed inlet, and the culture chamber is provided with a culture control assembly; the culture control assembly comprises a sliding door driving cylinder arranged on a culture cavity, the output end of the sliding door driving cylinder is connected with a sliding door, the sliding door is arranged on a feeding hole of the culture cavity in a sliding mode, a sliding door rack is arranged on the sliding door, an upper rack and a lower rack are arranged on the sliding door rack, the upper rack on the sliding door rack is matched with a placing driving gear, the lower rack on the sliding door rack is matched with a transposition driving gear, the placing driving gear is arranged at one end of a connecting shaft I, the transposition driving gear is arranged at one end of a connecting shaft II, the connecting shaft I and the connecting shaft II are both rotatably arranged on the culture cavity, the other end of the connecting shaft I is connected with a screw rod of a placing table through a connecting belt I, the other end of the connecting shaft II is connected with a ratchet wheel through a connecting belt II, the ratchet wheel is rotatably arranged on an incubator shell, the ratchet wheel is matched with a pawl on the inner side of a synchronous belt I, and the synchronous belt I is rotatably arranged on the incubator shell, the first synchronous belt wheel is connected with the second synchronous belt wheel through a synchronous belt, the second synchronous belt wheel is rotatably arranged on an incubator shell, the second synchronous belt wheel is connected with a transition gear, the transition gear is matched with a transposition master gear, the transposition master gear is rotatably arranged on the incubator shell, one end of a transposition connecting rod is rotatably arranged on the transposition master gear, the transposition connecting rods are provided with a plurality of transposition connecting rods and are uniformly distributed along the circumference of the transposition master gear, all the transposition connecting rods are vertically arranged, the other ends of all the transposition connecting rods are rotatably arranged on a transposition wheel and are uniformly distributed, the other end of the transposition connecting rod is fixedly connected with a placing platform sliding frame, the transposition wheel is rotatably arranged on a culture cavity, a placing platform is slidably arranged on the placing platform sliding frame, a culture dish is placed on the placing platform, a connecting groove is arranged on the placing platform, the connecting groove is matched with a placing platform driving rod, and a threaded hole and a guide hole are arranged on the placing platform driving rod, and respectively with place a screw rod and place a cooperation of a guide arm, place a guide arm and place a screw rod and all set up in cultivateing the intracavity.
Further, a weight sensor is arranged on the placing table.
Furthermore, the input control assembly comprises a fixture block driving cylinder arranged on the input cylinder, the output end of the fixture block driving cylinder is connected with the fixture block, the fixture block is matched with the sealing handle, the sealing handle is connected with the sealing sheet, and the sealing sheet is rotatably arranged on the input cylinder through a torsion spring.
Further, the subassembly of taking a breath is including rotating the revolving stage of taking a breath that sets up on the incubator shell, is provided with the filter core mounting hole on the revolving stage of taking a breath, installs air filter in the filter core mounting hole, and the filter core mounting hole of revolving stage of taking a breath corresponds with the vent of the changeover portion of taking a breath, and the changeover portion of taking a breath sets up in the incubator shell, is provided with the scavenger fan on the vent, and the scavenger fan is through the motor drive of taking a breath.
Furthermore, a ventilation handle is arranged on the ventilation rotary table, the ventilation fan is connected with one end of a ventilation driving shaft, the other end of the ventilation driving shaft is connected with a first rotating wheel through a ventilation driving belt, the first rotating wheel is rotatably arranged in the incubator shell, the first rotating wheel is matched with a second rotating wheel, the second rotating wheel is arranged on an output shaft of a ventilation motor, the ventilation motor is arranged on a ventilation sliding seat, the ventilation sliding seat is slidably mounted on a ventilation sliding rail, the ventilation sliding rail is arranged in the incubator shell, a threaded hole is formed in the ventilation sliding seat and is matched with a ventilation driving screw, one end of the ventilation driving screw is rotatably arranged in the incubator shell, and the other end of the ventilation driving screw is coaxially connected with the ventilation rotary table.
Furthermore, the input assembly comprises an input screw which is rotatably arranged in an input cylinder, one end of the input screw is connected with an output shaft of a screw motor, the screw motor is arranged in the input cylinder, an input guide rod is further arranged in the input cylinder, a threaded hole and a guide hole are formed in the input sliding seat and are respectively matched with the input screw and the input guide rod, a moving seat is arranged on the input sliding seat in a sliding mode, a reset spring is connected between the input sliding seat and the moving seat, a clamping jaw driving cylinder is arranged on the moving seat and connected with a clamping jaw, a pipe hole is formed in the moving seat, an inclined block is arranged in the input cylinder, and the inclined block is in contact fit with the moving seat.
Further, the suction assembly comprises a lifting driving cylinder arranged in the incubator shell, the output end of the lifting driving cylinder is connected with the fixing frame, a liquid suction pipe is arranged on the fixing frame, the input end of the liquid suction pipe penetrates through a rectangular sliding groove in the input cylinder and is arranged in the input cylinder, a sealing sheet is arranged on the liquid suction pipe and is in sliding fit with the input cylinder, one end of a liquid discharge pipe is connected onto the liquid suction pipe, a valve II is arranged on the liquid discharge pipe, a valve I is arranged on the liquid suction pipe, a piston is arranged in the other end of the liquid suction pipe, the piston is connected with the output end of the liquid suction driving cylinder through a piston connecting rod, the liquid suction driving cylinder is arranged on the fixing frame, and the other end of the liquid discharge pipe is arranged on the upper side of a material inlet of the culture cavity.
Further, the pipette and drain have a diameter of less than 1cm.
Compared with the prior art, the invention has the beneficial effects that: (1) The input assembly provided by the invention can automatically send the placed culture tubes to the lower side of the input end of the suction assembly, and can enable the empty culture tubes to fall into the recovery box for recovery; (2) The air exchange assembly provided by the invention can replace the air filter element at any time under the condition that the equipment is not shut down, so that the interior of the equipment is maintained in a sterile state; (3) The suction assembly provided by the invention can automatically switch the passage, and the sucked solution is sent into the culture dish from the culture tube; (4) The culture control assembly provided by the invention can enable the placing table to extend out while opening the sliding door, and can automatically change positions when the placing table is retracted; (5) The input control assembly arranged in the invention can be matched with the weight sensor on the placing table, and the sealing sheets are automatically closed after all the culture dishes on the placing table are added with the solution.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic view of the overall structure of the present invention.
FIG. 3 is a schematic top view of the input control module according to the present invention.
FIG. 4 is a schematic view of the internal structure of the incubator casing according to the present invention.
Fig. 5 is a schematic view of the internal structure of the input cylinder according to the first embodiment of the present invention.
Fig. 6 is an enlarged view of a structure shown in fig. 5.
Fig. 7 is a schematic diagram of the internal structure of the input cylinder of the present invention.
Fig. 8 is an enlarged view of the structure at B in fig. 7.
Fig. 9 is a partial structural view of the ventilation assembly of the present invention.
FIG. 10 is a second schematic view of the internal structure of the incubator casing according to the present invention.
Fig. 11 is an enlarged view of the structure at C in fig. 10.
FIG. 12 is a third schematic view of the internal structure of the incubator casing according to the present invention.
Fig. 13 is an enlarged view of the structure at D in fig. 12.
FIG. 14 is a fourth schematic view of the internal structure of the incubator housing according to the present invention.
FIG. 15 is a fifth schematic view of the internal structure of the incubator housing according to the present invention.
Fig. 16 is an enlarged view of the structure at E in fig. 15.
FIG. 17 is a sixth schematic view of the internal structure of the incubator casing according to the present invention.
Fig. 18 is an enlarged view of the structure at F in fig. 17.
FIG. 19 is a seventh schematic view showing the internal structure of the case of the incubator of the present invention.
Fig. 20 is an enlarged view of the structure at G in fig. 19.
Reference numbers: 1-incubator housing; 2-sliding door of incubator; 3-an input cylinder; 4-recovery box; 5-input control component; 6-a ventilation assembly; 7-an input component; 8-a suction assembly; 9-a culture control assembly; 10-a culture chamber; 11-a fixture block driving cylinder; 12-a fixture block; 13-sealing a handle; 14-a sealing sheet; 15-input screw; 16-an input guide rod; 17-an input slide; 18-a mobile seat; 19-a return spring; 20-a jaw actuating cylinder; 21-a clamping jaw; 22-a screw motor; 23-a sloping block; 24-a ventilation rotary table; 25-air exchanging handle; 26-an air filter element; 27-a ventilation transition chamber; 28-a ventilator; 29-a ventilation drive shaft; 30-ventilation driving belt; 31-a first rotating wheel; 32-rotating wheel two; 33-a ventilation motor; 34-air change slide rail; 35-a ventilation slide seat; 36-a scavenging drive screw; 37-lifting driving cylinder; 38-a mount; 39-pipette; 40-sealing slice; 41-valve one; 42-valve two; 43-liquid discharge pipe; 44-a liquid suction driving cylinder; 45-piston connecting rod; 46-sliding door actuating cylinder; 47-a sliding door; 48-sliding door rack; 49-place drive gear; 50-connecting the shaft I; 51-a first gearbox; 52-connecting shaft II; 53-gearbox two; 54-index drive gear; 55-connecting a first belt; 56-connecting a second belt; 57-ratchet wheel; 58-synchronous pulley one; 59-synchronous belt; 60-a second synchronous pulley; 61-transition gear; 62-shifting the main gear; 63-transposition connecting rods; 64-a transposition wheel; 65-placing table guide rods; 66-placing table screw; 67-placing table drive rods; 68-placing the table carriage; 69-placing the table.
Detailed Description
In the following description of the present invention, it is to be noted that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the following description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; the two components can be directly connected or indirectly connected through an intermediate medium, and the two components can be communicated with each other. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The invention will be further described with reference to the drawings and illustrative embodiments, which are provided herein to illustrate and not to limit the invention. Wherein like reference numerals refer to like parts throughout. In addition, if a detailed description of the known art is not necessary to show the features of the present invention, it is omitted.
The embodiment is as follows: referring to fig. 1-20, a cell culture apparatus for cell biology experiments is shown, wherein a sliding door 2 of an incubator is disposed on a top surface of an incubator housing 1, a culture chamber 10 is disposed on a lower side of the sliding door 2 of the incubator, the culture chamber 10 is fixedly disposed in the incubator housing 1, the culture chamber 10 opens upward on the top surface of the incubator housing 1 and is matched with the sliding door 2 of the incubator, an input cylinder 3 is penetratingly disposed on the incubator housing 1, one end of the input cylinder 3 located on an upper side of the incubator housing 1 is longer, one end of the input cylinder 3 located on the lower side of the incubator housing 1 is connected with a recovery box 4, the recovery box 4 is disposed on the lower side of the incubator housing 1, an input control component 5 is disposed on a top end of the input cylinder 3, an input component 7 is disposed in the input cylinder 3, a ventilation component 6 is further disposed on the incubator housing 1, a suction component 8 is disposed in the incubator housing 1, one end of the input of the suction component 8 is disposed inside the input cylinder 3 through the input cylinder 3, a material inlet is disposed on a portion of the incubator housing 1, one end of the output of the culture chamber 10 is disposed on an upper side of the incubator housing 10, and a material inlet 9 is disposed on the culture chamber 10.
Referring to fig. 1 and fig. 4, the input control assembly 5 includes a fixture block driving cylinder 11, a fixture block 12, a sealing handle 13, and a sealing plate 14, wherein the fixture block driving cylinder 11 is fixedly disposed on an outer wall of the input cylinder 3, an output end of the fixture block driving cylinder 11 is fixedly connected to the fixture block 12, the fixture block 12 is matched with the sealing handle 13, the sealing handle 13 is fixedly connected to the sealing plate 14, the sealing plate 14 is rotatably disposed at a top end of the input cylinder 3 through a torsion spring, and the sealing plate 14 is tightly attached to an opening at the top end of the input cylinder 3.
Referring to fig. 2 and fig. 9-11, a ventilation assembly 6 is shown, which includes a ventilation rotary table 24, a ventilation handle 25, an air filter 26, a ventilation transition chamber 27, a ventilation fan 28, a ventilation driving shaft 29, a ventilation driving belt 30, a first rotating wheel 31, a second rotating wheel 32, a ventilation motor 33, a ventilation slide rail 34, a ventilation slide carriage 35, and a ventilation driving screw 36, wherein the ventilation rotary table 24 passes through the incubator casing 1 and is rotatably disposed on the outer wall of the incubator casing 1, the ventilation rotary table 24 is provided with a filter mounting hole axially penetrating therethrough, the filter mounting hole is eccentrically disposed on the ventilation rotary table 24, the air filter 26 is detachably mounted in the filter mounting hole, the ventilation rotary table 24 is fixedly provided with the ventilation handle 25 on the side outside the incubator casing 1, the ventilation rotary table 24 is disposed in the ventilation transition chamber 27, the ventilation transition chamber 27 is fixedly disposed in the incubator casing 1, the ventilation transition chamber 27 is provided with a ventilation opening corresponding to the filter mounting hole, the ventilation opening is disposed on the side of the ventilation rotary table casing 1 facing the incubator casing 1, the ventilation rotary table 24 is connected to one end of the ventilation rotary table 29, the ventilation rotary table 34 is disposed on the rotary slide carriage 35, the ventilation slide rail 34, the ventilation slide carriage 35, the ventilation driving shaft 31, the ventilation slide carriage 35 is disposed on the ventilation slide rail 31, the ventilation slide rail 35, the ventilation driving shaft 31, the ventilation rotary table 35 is disposed in the ventilation rotary table 35, the other end of the ventilation driving screw 36 is coaxially and fixedly connected with the ventilation rotary table 24.
Referring to fig. 3 and fig. 5-8, the input assembly 7 shown in fig. 3 includes an input screw 15, an input guide rod 16, an input slide carriage 17, a moving seat 18, a return spring 19, a jaw driving cylinder 20, a jaw 21, a screw motor 22, and an inclined block 23, wherein the input screw 15 is rotatably disposed in the input cylinder 3, one end of the input screw 15 is fixedly connected to an output shaft of the screw motor 22, the screw motor 22 is fixedly disposed in the input cylinder 3, the input guide rod 16 is also fixedly disposed in the input cylinder 3, the input slide carriage 17 is provided with a threaded hole and a guide hole, the threaded hole of the input slide carriage 17 and the input screw 15 form a threaded fit, the guide hole of the input slide carriage 17 and the input guide rod 16 form a sliding fit, the input slide carriage 17 is provided with a moving seat 18 in a sliding manner, the return spring 19 is connected between the input slide carriage 17 and the moving seat 18, the jaw driving cylinder 20 is fixedly disposed on the moving seat 18, the jaw driving cylinder 20 is connected to the jaw 21, the jaw 21 is located on the upper side of the moving seat 18, a place corresponding to the jaw 21 is disposed on the moving seat 18, the lower end of the input screw 15 and the input guide rod 16 is disposed on the inclined block 23, and the inclined block 23 is in contact with the inner wall of the moving seat 3.
Referring to FIG. 4 and FIGS. 12-13, the sucking assembly 8 comprises a lifting driving cylinder 37, a fixing frame 38, a liquid discharging pipe 43, a liquid sucking driving cylinder 44, and a piston connecting rod 45, wherein the lifting driving cylinder 37 is fixedly arranged on the inner bottom surface of the incubator casing 1, the output end of the lifting driving cylinder 37 is fixedly connected with the fixing frame 38, the liquid sucking pipe 39 is fixedly arranged on the fixing frame 38, the liquid sucking pipe 39 is in a shape like a Chinese character 'ji', the input end of the liquid sucking pipe 39 passes through the input cylinder 3 and is arranged in the input cylinder 3, the input cylinder 3 is provided with a longitudinal rectangular chute, the liquid sucking pipe 39 is slidably connected with the rectangular chute, the liquid sucking pipe 39 is fixedly provided with the sealing plate 40, and the sealing plate 40 is closely attached to and slidably matched with the outer wall of the input cylinder 3, and the pipette 39 is positioned at any position of the rectangular chute, the length of the sealing sheet 40 can completely cover the rectangular chute, one end of a liquid discharge pipe 43 is connected to the middle section of the pipette 39, a second valve 42 is arranged at one end of the liquid discharge pipe 43 connected with the pipette 39, a first valve 41 is arranged at one side of the pipette 39, which is positioned at the joint of the pipette 43 and close to the input end of the pipette 39, a piston is arranged in the other end of the pipette 39, the piston is connected with the output end of a liquid suction driving cylinder 44 through a piston connecting rod 45, the liquid suction driving cylinder 44 is fixedly arranged on the fixed frame 38, the other end of the liquid discharge pipe 43 is arranged at the upper side of the feeding port of the culture cavity 10, and the diameter of the pipette 39 and the liquid discharge pipe 43 is less than 1cm.
Referring to FIG. 4 and FIG. 14-FIG. 20, the cultivation control assembly 9 comprises a sliding door driving cylinder 46, a sliding door 47, a sliding door rack 48, a placing driving gear 49, a connecting shaft 50, a gear box 51, a connecting shaft 52, a gear box 53, a transposition driving gear 54, a connecting belt 55, a connecting belt 56, a ratchet wheel 57, a synchronous pulley 58, a synchronous belt 59, a synchronous pulley 60, a transition gear 61, a transposition master gear 62, a transposition connecting rod 63, a transposition wheel 64, a placing table guide rod 65, a placing table screw 66, a placing table driving rod 67, a placing table carriage 68 and a placing table 69, wherein the sliding door driving cylinder 46 is fixedly arranged on the cultivation chamber 10, the output end of the sliding door driving cylinder 46 is fixedly connected with the sliding door 47, the sliding door 47 is slidably arranged on the feeding port of the cultivation chamber 10, a rack groove is formed on the upper side of the feeding port, the sliding door rack 48 is slidably arranged in the rack groove, a sliding door rack 48 is fixedly arranged on the sliding door 47, an upper rack and a lower rack are arranged on the sliding door rack 48, a section of the rack on the upper side of the sliding door rack 48 on one end far away from the sliding door driving cylinder 46 is not arranged, a section of the rack on the lower side of the sliding door rack 48 is only arranged on one section of one end far away from the sliding door driving cylinder 46, racks on the upper side and the lower side of the sliding door rack 48 are complementary, the rack on the upper side of the sliding door rack 48 and a placing driving gear 49 form a rack-and-pinion fit, the rack on the lower side of the sliding door rack 48 and a transposition driving gear 54 form a rack-and-pinion fit, the placing driving gear 49 is fixedly arranged on one end of a connecting shaft one 50, the transposition driving gear 54 is fixedly arranged on one end of a connecting shaft two 52, the connecting shaft one 50 and the two connecting shafts 52 are both rotatably arranged outside the culture chamber 10, and a first gearbox 51 and a second gearbox 53 are respectively arranged on the connecting shaft 50 and the connecting shaft two 52, the other end of the first connecting shaft 50 is connected with a screw 66 of a placing platform through a first connecting belt 55, the other end of the second connecting shaft 52 is connected with a ratchet 57 through a second connecting belt 56, the ratchet 57 is rotatably arranged on the inner wall of the incubator shell 1, the ratchet 57 is simultaneously matched with a pawl on the inner side of a first synchronous pulley 58, the first synchronous pulley 58 is also rotatably arranged on the incubator shell 1, the first synchronous pulley 58 is connected with a second synchronous pulley 60 through a synchronous belt 59, the second synchronous pulley 60 is rotatably arranged on the incubator shell 1, the second synchronous pulley 60 is simultaneously and fixedly connected with a transition gear 61, the transition gear 61 is in gear fit with a transposition main gear 62, the transposition main gear 62 is rotatably arranged on the incubator shell 1, one end of a transposition connecting rod 63 is rotatably arranged on the transposition main gear 62, the transposition connecting rods 63 are provided with a plurality of gears and are uniformly distributed along the circumference of the transposition main gear 62, all the transposition connecting rods 63 are vertically arranged, the other ends of all the transposition connecting rods 63 are rotatably arranged on the transposition wheel 64 and fixedly connected with a placing table carriage 68 through the transposition wheel 64, the placing table carriage 68 is horizontally arranged, the other ends of the transposition connecting rods 63 are also uniformly distributed on the transposition wheel 64 in a circumferential manner, the transposition wheel 64 is rotatably arranged on the culture chamber 10, the placing table carriage 68 is arranged in the culture chamber 10, a placing table 69 is slidably arranged on the placing table carriage 68, a culture dish is placed on the placing table 69, a connecting groove is arranged on one side of the placing table 69, which is close to the side where the placing table carriage 68 is connected with the transposition wheel 64, the connecting groove is matched with the placing table driving rod 67, a threaded hole and a guide hole are arranged on the placing table driving rod 67, the threaded hole on the placing table driving rod 67 is in threaded fit with the placing table screw 66, the placing table screw 66 is rotatably arranged in the culture chamber 10, the guide hole on the placing table driving rod 67 is in sliding fit with the placing table guide rod 65, the placing table guide rod 65 is fixedly arranged in the culture chamber 10, and the placing table 69 is provided with a weight sensor which can transmit signals to the fixture block driving cylinder 11.
In the implementation of this embodiment, the sealing handle 13 is firstly moved, the sealing handle 13 drives the sealing sheet 14 to rotate and open, then the culture tube containing the prepared culture solution with cells is placed at the position of the clamping jaw 21, the clamping jaw driving cylinder 20 will drive the clamping jaw 21 to clamp the culture tube, at this time, the sealing handle 13 is loosened, the sealing sheet 14 will automatically reset through the action of the torsion spring, after the culture tube is clamped, the screw motor 22 drives the input screw 15 to rotate, the input sliding seat 17 and the moving seat 18 are driven to descend along the input guide rod 16, when the moving seat 18 descends to the position of the inclined block 23, the inclined block 23 will make the moving seat 18 slide and extend out along the input sliding seat 17, and stretch the reset spring 19, and simultaneously drive the culture tube to the lower side of the input end of the pipette 39, at this time, the lifting driving cylinder 37 drives the fixed mount 38 to descend, the fixed mount 38 drives the pipette 39 to descend, the input end of a pipette 39 extends into the culture tube, the pipette driving cylinder 44 is driven after the input end extends into the culture tube, the pipette driving cylinder 44 drives a piston in the other end of the pipette 39 to slide through a piston connecting rod 45, so that the solution in the culture tube is sucked along the pipette 39, the diameters of the pipette 39 and a liquid discharge tube 43 are smaller than 1cm, the pipette 39 and the liquid discharge tube 43 can play the same role as that of the pipette, at the moment, the states of a first valve 41 and a second valve 42 are respectively opened and closed, when the solution is completely sucked, the clamping jaw driving cylinder 20 drives a clamping jaw 21 to be loosened, so that the empty culture tube falls into a recovery box 4 through a tube falling hole, then a screw motor 22 drives an input sliding seat 17 to ascend again, a moving seat 18 automatically resets under the action of a reset spring 19, when the sucked solution completely passes through the first valve 41, the first valve 41 is closed and the second valve 42 is opened, the drain pipe 43 is now in communication with the pipette 39.
When the solution is completely sucked, the sliding door driving cylinder 46 drives the sliding door 47 to slide, the sliding door 47 slides to expose the feeding port of the culture chamber 10, the sliding door rack 48 slides synchronously and is matched with the placing driving gear 49 and the transposition driving gear 54, when the sliding door rack 48 slides out, the rack on the lower side is firstly matched with the transposition driving gear 54, the transposition driving gear 54 drives the ratchet wheel 57 to rotate through the connecting shaft II 52 and the connecting belt II 56, at the moment, the ratchet wheel 57 rotates clockwise (referring to the direction shown in figure 18), the ratchet wheel 57 does not match with the pawl when rotating clockwise, at the moment, the synchronous belt wheel I58 does not rotate, the sliding door rack 48 continuously slides out, the rack on the lower side of the sliding door rack 48 is completely matched, the rack on the upper side of the sliding door rack 48 is matched with the placing driving gear 49, the placing driving gear 49 rotates to drive the placing table screw 66 to rotate through the connecting shaft I50 and the connecting belt I55, the placing table screw 66 drives the placing table driving rod 67 to slide along the placing table guide rod 65, at the moment, the placing table driving rod 67 and the connecting groove of the placing table 69 are in a matching state, the placing table driving rod 67 will drive the placing table 69 to slide along the placing table sliding frame 68, a culture dish is placed on the upper side of the placing table 69, the placing table 69 drives the culture dish to extend out from the feeding port of the culture cavity 10, at the moment, the imbibition driving cylinder 44 is driven reversely, the piston in the imbibition pipe 39 is driven by the piston connecting rod 45 to move upwards, the solution in the imbibition pipe 39 is pushed out from the liquid discharge pipe 43, the solution will flow into the culture dish through the liquid discharge pipe 43, at the moment, the second valve 42 is closed, the first valve 41 is opened, the sliding door driving cylinder 46 drives the sliding door 47 to withdraw after the culture dish is filled, the sliding door 47 drives the sliding door rack 48 to withdraw, and during the withdrawing of the sliding door rack 48, the upper side rack is firstly matched with the placing driving gear 49, the placing driving gear 49 drives the placing table screw 66 to rotate reversely through transmission, at this time, the placing table driving rod 67 drives the placing table 69 to retract along the placing table carriage 68 through the connecting groove of the placing table 69, after the placing table driving rod 67 returns to the initial position, the rack on the upper side of the placing table carriage 68 is engaged, at this time, the rack on the lower side of the placing table carriage 68 is engaged with the transposition driving gear 54, the transposition driving gear 54 drives the ratchet 57 to rotate counterclockwise (refer to the direction shown in fig. 18) through transmission, at this time, the ratchet 57 is engaged with the pawl, the synchronous pulley one 58 rotates along with the rotation, the synchronous pulley one 58 drives the synchronous pulley two 60 to rotate through the synchronous belt 59, the synchronous pulley two 60 drives the transition gear 61 to rotate, the transition gear 61 is engaged with the transposition main gear 62 to drive the transposition main gear 62 to rotate, the transposition main gear 62 drives the transposition wheel 64 to rotate through the transposition connecting rod 63, the transposition wheel 64 rotates to drive the placing table carriages 68 to rotate for transposition, the transposition connecting rod 63 is vertically arranged and is always in a vertical state in the movement process, so that the placing table carriages 68 are always in a horizontal state in the rotation process, the placing table carriages 68 drive the placing tables 69 to rotate, the connecting grooves on the placing tables 69 are through up and down, the placing tables 69 at the positions are disconnected with the placing table driving rod 67 at the moment, the connecting grooves of the placing tables 69 at the next positions are matched with the placing table driving rod 67, the sliding door 47 is completely closed, the next process can be started, when all the culture dishes on the placing tables 69 are filled, the weight sensor on the placing tables 69 transmits signals to the fixture block driving cylinder 11, the fixture block driving cylinder 11 drives the fixture block 12 to extend out, the fixture block 12 clamps the sealing handle 13, and the sealing sheet 14 cannot be opened again at the moment, can not be put into the culture tube any more.
The space in the incubator shell 1 is a sterile space, so that the incubator shell is completely isolated from the outside, air exchange is performed only through the air exchange assembly 6, the air filter element 26 is arranged on the air exchange rotary table 24, the air exchange fan 28 is driven to exchange air through the air exchange motor 33, when the air filter element 26 needs to be replaced, the air exchange handle 25 is rotated, in order to ensure the airtightness of the space in the incubator shell 1, a filter element mounting hole in the air exchange rotary table 24 is staggered with a vent hole in the air exchange transition cavity 27, the air exchange rotary table 24 blocks the vent hole, the air exchange drive screw 36 is driven to rotate simultaneously in the rotating process of the air exchange rotary table 24, the air exchange drive screw 36 is matched with the air exchange slide seat 35 to drive the air exchange slide seat 35 to slide on the air exchange slide rail 34, the air exchange slide seat 35 drives the air exchange motor 33 to slide, the air exchange motor 33 slides to disconnect the rotating wheels 32 from the rotating wheels one 31, at the moment, the air exchange fan 28 stops working, after the air filter element 26 is replaced, the air exchange handle 25 is rotated again to reset, at any time, the air exchange motor 33 resets, the rotating wheels 32 is reconnected with the rotating wheels 31, the air exchange fan 28 restarts, the work is not needed to replace the filter element 26, and the air filter element can be replaced.
It should be understood that the above embodiments are only for illustrating the technical solutions of the present invention, and not for limiting the same, and those skilled in the art can modify the technical solutions described in the above embodiments, or make equivalent substitutions for some technical features; and all such modifications and substitutions are intended to be included within the scope of the present invention as defined in the appended claims.
Claims (8)
1. The utility model provides a cell culture device for cell biology experiments, includes incubator shell (1), be provided with incubator sliding door (2) and subassembly (6) of taking a breath on incubator shell (1), its characterized in that: a culture cavity (10) is arranged in the incubator shell (1) and is positioned on the lower side of the incubator sliding door (2), an input cylinder (3) is arranged on the incubator shell (1), an input control component (5) is arranged at the top end of the input cylinder (3), the bottom end of the input cylinder (3) is connected with a recovery box (4), the recovery box (4) is arranged on the lower side of the incubator shell (1), an input component (7) is arranged in the input cylinder (3), a suction component (8) is arranged in the incubator shell (1), the input end of the suction component (8) is arranged in the input cylinder (3), a feeding hole is formed in the culture cavity (10), the output end of the suction component (8) is arranged on the upper side of the feeding hole, and a culture control component (9) is arranged on the culture cavity (10);
the culture control assembly (9) comprises a sliding door driving cylinder (46) arranged on a culture chamber (10), the output end of the sliding door driving cylinder (46) is connected with a sliding door (47), the sliding door (47) is arranged on a feed inlet of the culture chamber (10) in a sliding manner, a sliding door rack (48) is arranged on the sliding door (47), an upper rack and a lower rack are arranged on the sliding door rack (48), a rack on the upper side of the sliding door rack (48) is matched with a placing driving gear (49), a rack on the lower side of the sliding door rack (48) is matched with a transposition driving gear (54), the placing driving gear (49) is arranged on one end of a connecting shaft I (50), a transposition driving gear (54) is arranged on one end of a connecting shaft II (52), a connecting shaft I (50) and a connecting shaft II (52) are both rotatably arranged on the culture chamber (10), the other end of the first connecting shaft I (50) is connected with a placing table screw rod (66) through a connecting belt, the other end of the second connecting shaft (56) is connected with a ratchet wheel II (57), the connecting shaft (57) is rotatably arranged on a synchronous pulley (58) of a culture box (1, a synchronous pulley (58) and a synchronous pulley (58) is arranged on a synchronous pulley (58), the second synchronous belt wheel (60) is rotatably arranged on the incubator shell (1), the second synchronous belt wheel (60) is connected with a transition gear (61), the transition gear (61) is matched with a transposition master gear (62), the transposition master gear (62) is rotatably arranged on the incubator shell (1), one end of a transposition connecting rod (63) is rotatably arranged on the transposition master gear (62), the transposition connecting rod (63) is provided with a plurality of transposition connecting rods and is uniformly distributed along the circumference of the transposition master gear (62), all the transposition connecting rods (63) are vertically arranged, the other ends of all the transposition connecting rods (63) are rotatably arranged on the transposition wheel (64) and are uniformly distributed, the other end of the transposition connecting rod (63) is fixedly connected with a placing platform (68), the transposition wheel (64) is rotatably arranged on the incubator cavity (10), a placing platform (69) is slidably arranged on the placing platform carriage (68), a culture dish is placed on the placing platform (69), a connecting groove is arranged on the placing platform (69), the connecting groove is matched with the placing platform (67), a driving rod (67) and a guide rod (65) are respectively matched with a placing platform (10).
2. The cell culture apparatus according to claim 1, wherein: the placing table (69) is provided with a weight sensor.
3. The cell culture apparatus according to claim 1, wherein: the input control assembly (5) comprises a fixture block driving cylinder (11) arranged on the input cylinder (3), the output end of the fixture block driving cylinder (11) is connected with a fixture block (12), the fixture block (12) is matched with a sealing handle (13), the sealing handle (13) is connected with a sealing sheet (14), and the sealing sheet (14) is rotatably arranged on the input cylinder (3) through a torsion spring.
4. The cell culture apparatus according to claim 1, wherein: subassembly (6) of taking a breath is including rotating revolving stage (24) of taking a breath that sets up on incubator shell (1), is provided with the filter core mounting hole on the revolving stage (24) of taking a breath, installs air filter core (26) in the filter core mounting hole, and the filter core mounting hole of revolving stage (24) of taking a breath corresponds with the vent of transition chamber (27) of taking a breath, and transition chamber (27) of taking a breath set up in incubator shell (1), is provided with scavenger fan (28) on the vent, and scavenger fan (28) are through the drive of air exchange motor (33).
5. The cell culture apparatus for cell biology experiments, according to claim 4, wherein: the air exchange rotary table is characterized in that an air exchange handle (25) is arranged on the air exchange rotary table (24), the air exchange fan (28) is connected with one end of an air exchange driving shaft (29), the other end of the air exchange driving shaft (29) is connected with a first rotating wheel (31) through an air exchange driving belt (30), the first rotating wheel (31) is rotatably arranged in the incubator shell (1), the first rotating wheel (31) is matched with a second rotating wheel (32), the second rotating wheel (32) is arranged on an output shaft of an air exchange motor (33), the air exchange motor (33) is arranged on an air exchange sliding seat (35), the air exchange sliding seat (35) is slidably arranged on an air exchange sliding rail (34), the air exchange sliding rail (34) is arranged in the incubator shell (1), a threaded hole is formed in the air exchange sliding seat (35) and matched with an air exchange driving screw rod (36), one end of the air exchange driving screw rod (36) is rotatably arranged in the incubator shell (1), and the other end of the air exchange driving screw rod (36) is coaxially connected with the air exchange rotary table (24).
6. The cell culture apparatus according to claim 1, wherein: the input assembly (7) comprises an input screw (15) which is rotatably arranged in an input cylinder (3), one end of the input screw (15) is connected with an output shaft of a screw motor (22), the screw motor (22) is arranged in the input cylinder (3), an input guide rod (16) is further arranged in the input cylinder (3), a threaded hole and a guide hole are formed in an input sliding seat (17) and are respectively matched with the input screw (15) and the input guide rod (16), a moving seat (18) is arranged on the input sliding seat (17) in a sliding mode, a reset spring (19) is connected between the input sliding seat (17) and the moving seat (18), a clamping jaw driving cylinder (20) is arranged on the moving seat (18), the clamping jaw driving cylinder (20) is connected with a clamping jaw (21), a tube falling hole is formed in the moving seat (18), an inclined block (23) is arranged in the input cylinder (3), and the inclined block (23) is in contact fit with the moving seat (18).
7. The cell culture apparatus according to claim 1, wherein: the suction assembly (8) comprises a lifting driving cylinder (37) arranged in an incubator shell (1), the output end of the lifting driving cylinder (37) is connected with a fixing frame (38), a suction pipe (39) is arranged on the fixing frame (38), the input end of the suction pipe (39) penetrates through a rectangular sliding groove in an input cylinder (3) and is arranged in the input cylinder (3), a sealing sheet (40) is arranged on the suction pipe (39), the sealing sheet (40) is in sliding fit with the input cylinder (3), one end of a liquid discharge pipe (43) is connected to the suction pipe (39), a valve II (42) is arranged on the liquid discharge pipe (43), a valve I (41) is arranged on the suction pipe (39), a piston is arranged at the other end of the suction pipe (39), the piston is connected with the output end of the liquid suction driving cylinder (44) through a piston connecting rod (45), the liquid suction driving cylinder (44) is arranged on the fixing frame (38), and the other end of the liquid discharge pipe (43) is arranged on the upper side of a feeding port of the incubation cavity (10).
8. The cell culture apparatus according to claim 7, wherein: the diameter of the liquid suction pipe (39) and the liquid discharge pipe (43) is less than 1cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115772471A (en) * | 2023-02-13 | 2023-03-10 | 山东第一医科大学附属省立医院(山东省立医院) | Organ chip culture apparatus capable of adjusting oxygen concentration |
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