CN111635850A - Vertical directional screening device for strains - Google Patents

Vertical directional screening device for strains Download PDF

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Publication number
CN111635850A
CN111635850A CN202010361136.0A CN202010361136A CN111635850A CN 111635850 A CN111635850 A CN 111635850A CN 202010361136 A CN202010361136 A CN 202010361136A CN 111635850 A CN111635850 A CN 111635850A
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CN
China
Prior art keywords
screening
screening device
vertical directional
strains
stirring
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CN202010361136.0A
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Chinese (zh)
Inventor
王彬
赵飞
张元长
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Xiamen Jiasheng Biotechnology Co ltd
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Xiamen Jiasheng Biotechnology Co ltd
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Priority to CN202010361136.0A priority Critical patent/CN111635850A/en
Publication of CN111635850A publication Critical patent/CN111635850A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10Rotating vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of screening, and discloses a vertical directional screening device for strains, which comprises a fixed bottom plate, wherein support frames are arranged on two sides of the upper end of the fixed bottom plate, a top plate is arranged at the top end of each support frame, a rotary drum is arranged in the middle of the top plate through a bearing, a screening barrel is arranged at the bottom end of the rotary drum, the bottom end inside the screening barrel is connected with a stirring shaft through a rotary seat, the upper end of the stirring shaft penetrates through the rotary drum and is connected with an output shaft of a stirring motor, a driving box is arranged at the upper end of the top plate, a partition plate is arranged inside the driving box, the middle part of the partition plate is rotatably connected with the upper. According to the invention, the mother liquor in the screening barrel can be fully stirred, screened and separated, so that biological strains and waste residues can be effectively centrifuged, the layering phenomenon of the microbial strains and the waste residues is generated, the screening efficiency is high, and the operation is convenient and simple.

Description

Vertical directional screening device for strains
Technical Field
The invention relates to the technical field of screening, in particular to a vertical directional screening device for strains.
Background
The strain refers to microorganisms used as a living cell catalyst in the fermentation process, and comprises four categories of bacteria, actinomycetes, yeasts and molds. Is derived from a large number of microorganisms in the nature, useful strains are separated and screened from the microorganisms, and then the microorganisms are improved and stored for production.
The screening of bacterial strain is mainly characterized by that it utilizes the different requirements of growth and reproduction of different kinds of microorganisms on environment and nutrients, such as temp., pH value, osmotic pressure, oxygen, carbon source and nitrogen source, etc. to artificially control these conditions so as to make it favourable for growth of a certain kind of microorganism or a certain kind of microorganism, but not favourable for survival of other kinds of microorganisms, so that the goal of making the bacterial strain predominate can be quickly separated and purified. If the oxygen during the culture can be controlled, the aerobic microorganisms and the anaerobic microorganisms can be separated; by controlling the temperature, thermophilic and non-thermophilic microorganisms can be separated; controlling pH, separating acidophilic and alkalophilic microorganisms, etc. Different antibiotics or reagents may also be added to the separation medium to increase selectivity. For example, when separating actinomycetes and bacteria, antifungal antibiotics can be added; when isolating fungi, antibacterial agents may be added.
The separation and screening of microbial strains from nature is the most basic and main method for obtaining excellent new strains, so the separation and screening of strains in the food industry is an important, long-term and heavy work. Various microorganisms exist in the nature, and particularly, the microorganisms in the soil are complete in variety and are the major cause of the microorganisms. Almost all microorganisms can be isolated from the soil. In addition, the dominant species of microorganisms living in different natural environments are also different. Different foods, agricultural and sideline products and the like have different nutritional compositions, and different microorganisms can be separated from the foods, the agricultural and sideline products and the like. The separation sources commonly used for strains in the food industry are contaminated production or research strains, strains used for a long time in production, strains for fermented foods, animal intestinal flora, microbial materials treated by various breeding methods and natural strain samples.
Microorganisms in nature are mixed and live, and in order to separate a microorganism from the mixture, it is necessary to consider that the separation source should contain a large amount of target bacteria and separate the target bacteria from other strains in the separation operation. For the bacteria with low content in the sample, a separation method is reasonably designed according to the biological characteristics, such as enrichment culture, selective culture, specific growth phenomenon generated by utilizing the special physiological reaction of the bacteria, and the like, so as to select the target bacteria from a large amount of mixed bacteria. The existing separation adopts multi-step manual operation separation, the separation efficiency is low, time and labor are consumed, and the working efficiency of workers is influenced.
Chinese patent (publication No. CN 209292344U) discloses a microorganism bacterial screening device, which comprises a connecting rod and a box body, wherein the lower surface of the box body is fixedly connected with a plurality of supporting rods, the lower end surface of each supporting rod is fixedly connected with a damping device, the inner bottom surface of the box body is fixedly connected with a trapezoidal stop, the lower surface of the box body is fixedly connected with two screening pipes symmetrically arranged about the trapezoidal stop, a driving device is installed on one side surface inside the box body, the output end of the driving device is fixedly connected with an outer pipe, the lower end surface of the outer pipe is fixedly connected with a centrifugal tank, the outer side surface of the centrifugal tank is provided with a waste residue removing pipe and a strain leading-in pipe, although the device can realize the screening effect of microorganism bacterial to a certain extent, the screening effect.
Disclosure of Invention
The invention aims to provide a vertical directional screening device for strains, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a to vertical directional sieving mechanism for bacterial, includes PMKD, PMKD upper end both sides erection bracing frame, support frame top installation roof, and the roof middle part is passed through the bearing and is installed rotatory section of thick bamboo, and rotatory bobbin bottom installation screening bucket, the inside bottom of screening bucket are connected with the (mixing) shaft through the roating seat, and the (mixing) shaft upper end is passed rotatory section of thick bamboo and is connected with agitator motor's output shaft, roof upper end installation drive case, drive case internally mounted baffle, baffle middle part rotate and are connected with the upper end of rotatory section of thick bamboo, rotatory section of thick bamboo lower extreme mid-mounting driven gear, the driven gear right side is connected with the.
As a further scheme of the invention: the driving gear upper end installation drive shaft, drive shaft upper end installation driving motor, driving motor is fixed in baffle right side middle part.
As a further scheme of the invention: the stirring motor is fixed on the top end of the inner wall of the driving box.
As a further scheme of the invention: the driving motor and the stirring motor rotate in the same direction.
As a further scheme of the invention: and a plurality of stirring blades are arranged on the outer side of the stirring shaft.
As a further scheme of the invention: the upper end of the outer side of the screening barrel is provided with a feed hopper.
As a further scheme of the invention: and a liquid level observation window is arranged in the middle of the outer side of the screening barrel.
As a further scheme of the invention: the middle parts of the two sides of the screening barrel are provided with an upper discharging pipe and a lower discharging pipe which are different in height.
As a further scheme of the invention: the limiting mechanism comprises an upper limiting ring and a lower limiting ring, the upper limiting ring is fixed at the lower end of the screening barrel, and the lower limiting ring is fixed at the upper end of the fixed bottom plate.
As a still further scheme of the invention: go up spacing ring downside and be equipped with the trapezoidal piece, go up spacing ring upside and be equipped with the dovetail of mutually supporting with the trapezoidal piece.
Compared with the prior art, the invention has the beneficial effects that:
the utility model provides a vertical directional screening device is used to bacterial, rational in infrastructure, the modern design through the inside (mixing) shaft that sets up soon to opposite under the rotation state of screening bucket for the inside mother liquor of screening bucket can obtain abundant stirring and screening separation, makes biological bacterial and waste residue can obtain effectual centrifugal action, makes microbial bacterial and waste residue produce the layering phenomenon, and the screening efficiency is high, and convenient operation is simple, and the practicality is strong.
Drawings
Fig. 1 is a schematic structural diagram of a vertical directional screening device for strains.
Fig. 2 is a schematic structural diagram of a limiting mechanism in the vertical directional screening device for strains.
FIG. 3 is a schematic view of a structure of a screening barrel in a vertical directional screening apparatus for strains.
In the figure: 1-fixed bottom plate, 2-limiting mechanism, 3-lower discharging pipe, 4-upper discharging pipe, 5-screening barrel, 6-stirring blade, 7-stirring shaft, 8-supporting frame, 9-rotating seat, 10-top plate, 11-bearing, 12-rotating cylinder, 13-driven gear, 14-driving gear, 15-driving shaft, 16-driving motor, 17-partition plate, 18-stirring motor, 19-driving box, 20-upper limiting ring, 21-lower limiting ring, 22-trapezoidal block, 23-dovetail groove and 24-liquid level observation window.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
Example 1
Referring to fig. 1 to 3, in the embodiment of the invention, a vertical directional screening device for strains comprises a fixed bottom plate 1, wherein support frames 8 are arranged on two sides of the upper end of the fixed bottom plate 1 and used for supporting and fixing a top plate 10, the top end of each support frame 8 is provided with a top plate 10, the middle part of each top plate 10 is provided with a rotary drum 12 through a bearing 11, so that the rotary drum 12 can rotate along the top plate 10 under the action of the bearing 11, a screening drum 5 is arranged at the bottom end of the rotary drum 12, the middle parts of two sides of the screening drum 5 are provided with an upper discharge pipe 4 and a lower discharge pipe 3 which are different in height, the upper discharge pipe 4 and the lower discharge pipe 3 are just higher in height to distinguish a strain layer and a strain residue layer so as to realize screening and separation effects, the middle part of the outer side of the screening drum 5 is provided with a, the upper end of the outer side of the screening barrel 5 is provided with a feed hopper for leading bacterial liquid into the screening barrel 5, the outer side of the stirring shaft 7 is provided with a plurality of stirring blades 6 for performing sufficient stirring and separating effects on the bacterial liquid in the screening barrel 5, the bottom end of the inner part of the screening barrel 5 is connected with the stirring shaft 7 through a rotating seat 9, the upper end of the stirring shaft 7 penetrates through the rotating barrel 12 to be connected with an output shaft of a stirring motor 18 for driving the stirring shaft 7 to rotate, the upper end of the top plate 10 is provided with a driving box 19, the inner part of the driving box 19 is provided with a baffle 17, the middle part of the baffle 17 is rotatably connected with the upper end of the rotating barrel 12, the middle part of the lower end of the rotating barrel 12 is provided with a driven gear 13, the right side of the driven gear 13 is connected with a driving gear 14, the upper, driving motor 16 is fixed in baffle 17 right side middle part, agitator motor 18 is fixed in 19 inner wall tops of drive box, sets up baffle 17 and has realized the fixed action to driving motor 16 and agitator motor 18, driving motor 16 is the same with agitator motor 18's direction of rotation, and the direction of rotation that sets up driving motor 16 and agitator motor 18 is the same, can be so that the direction of rotation between (mixing) shaft 7 and the screening bucket 5 is opposite, has further increased the screening effect, screening bucket 5 lower extreme installation stop gear 2.
Example 2
In another embodiment of the invention, the device comprises a fixed bottom plate 1, wherein support frames 8 are arranged on two sides of the upper end of the fixed bottom plate 1, a top plate 10 is arranged at the top end of each support frame 8, a rotary drum 12 is arranged in the middle of the top plate 10 through a bearing 11, a screening barrel 5 is arranged at the bottom end of the rotary drum 12, an upper discharge pipe 4 and a lower discharge pipe 3 with different heights are arranged in the middle of two sides of the screening barrel 5, a liquid level observation window 24 is arranged in the middle of the outer side of the screening barrel 5, a feed hopper is arranged at the upper end of the outer side of the screening barrel 5, a plurality of stirring blades 6 are arranged on the outer side of the stirring shaft 7, the bottom end in the screening barrel 5 is connected with a stirring shaft 7 through a rotary seat 9, a plurality of stirring blades 6 are arranged on the outer side of the stirring shaft 7, the upper end of the, the middle part of the lower end of the rotary cylinder 12 is provided with a driven gear 13, the right side of the driven gear 13 is connected with a driving gear 14, the upper end of the driving gear 14 is provided with a driving shaft 15, the upper end of the driving shaft 15 is provided with a driving motor 16, the driving motor 16 is fixed at the middle part of the right side of the clapboard 17, the stirring motor 18 is fixed at the top end of the inner wall of the driving box 19, the rotating directions of the driving motor 16 and the stirring motor 18 are the same, and the lower end of the screening barrel 5 is provided with a limiting mechanism 2. the difference of the embodiment from the above embodiment is that the limiting mechanism 2 comprises an upper limiting ring 20 and a lower limiting ring 21, the upper limiting ring 20 is fixed at the lower end of the screening barrel 5, the lower limiting ring 21 is fixed at the upper end of the fixed bottom plate 1, the lower side of the upper limiting ring 20 is provided with a trapezoidal, not only be convenient for install, also ensured simultaneously that whole screening bucket 5 can not take place to rock on a large scale at rotatory in-process, further increased the reliability of device.
In the invention, during working, strains to be screened and mother liquor are placed in the screening barrel 5 together, then the stirring motor 18 and the driving motor 16 are started, at the moment, the screening barrel 5 rotates, and the stirring shaft 7 drives the stirring blades 6 to rotate in the direction opposite to the direction of the screening barrel 5, so that the screening and separating effects are further improved, after the screening and separating effects are realized by turning off the motor after rotating for a period of time and turning on the upper discharging pipe 4 and the lower discharging pipe 6 after standing for a period of time. The invention has reasonable structure and novel design, the stirring shaft 7 with opposite rotation directions is arranged in the screening barrel 5 in a rotating state, so that mother liquor in the screening barrel 5 can be fully stirred, screened and separated, biological strains and waste residues can be effectively centrifuged, the microbial strains and the waste residues are layered, the screening efficiency is high, the operation is convenient and simple, and the practicability is strong.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. The vertical directional screening device for the strains comprises a fixed base plate (1), wherein support frames (8) are arranged on two sides of the upper end of the fixed base plate (1), and is characterized in that a top plate (10) is arranged on the top end of each support frame (8), a rotary drum (12) is arranged in the middle of each top plate (10) through a bearing (11), a screening drum (5) is arranged at the bottom end of each rotary drum (12), the bottom end inside each screening drum (5) is connected with a stirring shaft (7) through a rotary seat (9), the upper end of each stirring shaft (7) penetrates through the corresponding rotary drum (12) and is connected with an output shaft of a stirring motor (18), a driving box (19) is arranged at the upper end of each top plate (10), a partition plate (17) is arranged inside each driving box (19), the middle of each partition plate (17) is rotatably connected with the upper end of the corresponding rotary drum (12, and the lower end of the screening barrel (5) is provided with a limiting mechanism (2).
2. The vertical directional screening device for strains according to claim 1, wherein a driving shaft (15) is mounted at the upper end of the driving gear (14), a driving motor (16) is mounted at the upper end of the driving shaft (15), and the driving motor (16) is fixed in the middle of the right side of the partition plate (17).
3. The vertical directional screening device for strains according to claim 1, wherein the stirring motor (18) is fixed at the top end of the inner wall of the driving box (19).
4. The vertical oriented bacteria seed screening device as claimed in claim 2, wherein the driving motor (16) and the stirring motor (18) rotate in the same direction.
5. The vertical directional screening device for strains according to claim 1, wherein a plurality of stirring blades (6) are arranged outside the stirring shaft (7).
6. The vertical directional screening device for strains according to claim 1, wherein a feed hopper is arranged at the upper outer end of the screening barrel (5).
7. The vertical directional screening device for strains according to claim 1 or 6, wherein a liquid level observation window (24) is arranged in the middle of the outer side of the screening barrel (5).
8. The vertical directional screening device for bacterial seeds as claimed in claim 7, wherein the screening barrel (5) is provided with an upper discharging pipe (4) and a lower discharging pipe (3) at different heights in the middle of both sides.
9. The vertical directional screening device for strains according to claim 1, wherein the limiting mechanism (2) comprises an upper limiting ring (20) and a lower limiting ring (21), the upper limiting ring (20) is fixed at the lower end of the screening barrel (5), and the lower limiting ring (21) is fixed at the upper end of the fixed bottom plate (1).
10. The vertical directional screening device for strains according to claim 9, wherein a trapezoidal block (22) is arranged on the lower side of the upper limiting ring (20), and a dovetail groove (23) matched with the trapezoidal block (22) is arranged on the upper side of the upper limiting ring (20).
CN202010361136.0A 2020-04-30 2020-04-30 Vertical directional screening device for strains Pending CN111635850A (en)

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CN202010361136.0A CN111635850A (en) 2020-04-30 2020-04-30 Vertical directional screening device for strains

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Application Number Priority Date Filing Date Title
CN202010361136.0A CN111635850A (en) 2020-04-30 2020-04-30 Vertical directional screening device for strains

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112619843A (en) * 2020-12-28 2021-04-09 侯建锋 Pretreatment crushing and screening equipment for manufacturing waterproof mortar and preparation method of waterproof mortar

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006255626A (en) * 2005-03-18 2006-09-28 Kajima Corp Dry type anaerobic digester
CN108504450A (en) * 2018-04-02 2018-09-07 黄佳立 A kind of horizontal lead screw rotary type bioengineering animal tallow sap extraction device
CN208200914U (en) * 2018-03-27 2018-12-07 广东格物生物科技有限公司 Horizontal fermentor and probiotic production system
CN209292344U (en) * 2018-10-11 2019-08-23 天津泓锦程生物科技有限公司 A kind of microbe bacterial screening device
CN209490945U (en) * 2018-12-30 2019-10-15 民乐县陇上乡村农业科技有限公司 A kind of beverage production smashes filtering integrated device with pulp particle

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006255626A (en) * 2005-03-18 2006-09-28 Kajima Corp Dry type anaerobic digester
CN208200914U (en) * 2018-03-27 2018-12-07 广东格物生物科技有限公司 Horizontal fermentor and probiotic production system
CN108504450A (en) * 2018-04-02 2018-09-07 黄佳立 A kind of horizontal lead screw rotary type bioengineering animal tallow sap extraction device
CN209292344U (en) * 2018-10-11 2019-08-23 天津泓锦程生物科技有限公司 A kind of microbe bacterial screening device
CN209490945U (en) * 2018-12-30 2019-10-15 民乐县陇上乡村农业科技有限公司 A kind of beverage production smashes filtering integrated device with pulp particle

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112619843A (en) * 2020-12-28 2021-04-09 侯建锋 Pretreatment crushing and screening equipment for manufacturing waterproof mortar and preparation method of waterproof mortar

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Application publication date: 20200908

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