CN115281344A - Ganoderma lucidum polysaccharide composition for regulating female intestinal flora and preparation method thereof - Google Patents
Ganoderma lucidum polysaccharide composition for regulating female intestinal flora and preparation method thereof Download PDFInfo
- Publication number
- CN115281344A CN115281344A CN202210935355.4A CN202210935355A CN115281344A CN 115281344 A CN115281344 A CN 115281344A CN 202210935355 A CN202210935355 A CN 202210935355A CN 115281344 A CN115281344 A CN 115281344A
- Authority
- CN
- China
- Prior art keywords
- extract
- composition
- ganoderan
- ganoderma lucidum
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 86
- 150000004676 glycans Chemical class 0.000 title claims abstract description 53
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 53
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 53
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 45
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 45
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 28
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 58
- 239000000284 extract Substances 0.000 claims abstract description 55
- 235000011034 Rubus glaucus Nutrition 0.000 claims abstract description 45
- 235000009122 Rubus idaeus Nutrition 0.000 claims abstract description 45
- 240000007651 Rubus glaucus Species 0.000 claims abstract description 44
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 29
- 239000002994 raw material Substances 0.000 claims abstract description 25
- 240000000249 Morus alba Species 0.000 claims abstract description 22
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 22
- 239000009141 Houttuynia cordata plant extract Substances 0.000 claims abstract description 20
- 235000020235 chia seed Nutrition 0.000 claims abstract description 20
- 235000003145 Hippophae rhamnoides Nutrition 0.000 claims abstract description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- 241000229143 Hippophae Species 0.000 claims description 20
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 13
- 238000001035 drying Methods 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 12
- 235000013305 food Nutrition 0.000 claims description 11
- 239000000047 product Substances 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 239000002002 slurry Substances 0.000 claims description 9
- 238000002791 soaking Methods 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 230000002829 reductive effect Effects 0.000 claims description 8
- 238000007873 sieving Methods 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 240000000691 Houttuynia cordata Species 0.000 claims description 6
- 235000013719 Houttuynia cordata Nutrition 0.000 claims description 6
- 238000005520 cutting process Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000001694 spray drying Methods 0.000 claims description 6
- 235000003935 Hippophae Nutrition 0.000 claims description 5
- CWEZAWNPTYBADX-UHFFFAOYSA-N Procyanidin Natural products OC1C(OC2C(O)C(Oc3c2c(O)cc(O)c3C4C(O)C(Oc5cc(O)cc(O)c45)c6ccc(O)c(O)c6)c7ccc(O)c(O)c7)c8c(O)cc(O)cc8OC1c9ccc(O)c(O)c9 CWEZAWNPTYBADX-UHFFFAOYSA-N 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 5
- 229920002414 procyanidin Polymers 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 229920005654 Sephadex Polymers 0.000 claims description 3
- 239000012507 Sephadex™ Substances 0.000 claims description 3
- 230000033228 biological regulation Effects 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000000265 homogenisation Methods 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- 238000009210 therapy by ultrasound Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 235000012054 meals Nutrition 0.000 claims description 2
- 239000006187 pill Substances 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 2
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims 2
- 241000222336 Ganoderma Species 0.000 claims 1
- 239000002671 adjuvant Substances 0.000 claims 1
- 239000002417 nutraceutical Substances 0.000 claims 1
- 235000021436 nutraceutical agent Nutrition 0.000 claims 1
- 230000002265 prevention Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 21
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 abstract description 10
- 150000002772 monosaccharides Chemical class 0.000 abstract description 10
- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 7
- 239000008103 glucose Substances 0.000 abstract description 7
- 229940097043 glucuronic acid Drugs 0.000 abstract description 7
- 235000019640 taste Nutrition 0.000 abstract description 7
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 abstract description 6
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 abstract description 6
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 abstract description 6
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 abstract description 6
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 abstract description 6
- 229960002442 glucosamine Drugs 0.000 abstract description 6
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 abstract description 5
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 abstract description 5
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 abstract description 5
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 abstract description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 5
- 229930182830 galactose Natural products 0.000 abstract description 5
- 230000000295 complement effect Effects 0.000 abstract description 3
- 238000005457 optimization Methods 0.000 abstract description 3
- 238000013329 compounding Methods 0.000 abstract description 2
- AEMOLEFTQBMNLQ-AQKNRBDQSA-N D-glucopyranuronic acid Chemical compound OC1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-AQKNRBDQSA-N 0.000 abstract 1
- 240000000950 Hippophae rhamnoides Species 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 17
- 210000001035 gastrointestinal tract Anatomy 0.000 description 15
- 230000001965 increasing effect Effects 0.000 description 15
- 241000186000 Bifidobacterium Species 0.000 description 11
- 210000004347 intestinal mucosa Anatomy 0.000 description 10
- 235000013406 prebiotics Nutrition 0.000 description 10
- 239000006041 probiotic Substances 0.000 description 9
- 235000018291 probiotics Nutrition 0.000 description 9
- 241000192142 Proteobacteria Species 0.000 description 8
- 230000035943 smell Effects 0.000 description 8
- 241000606125 Bacteroides Species 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 150000004666 short chain fatty acids Chemical class 0.000 description 7
- 241000192125 Firmicutes Species 0.000 description 6
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- XFZJEEAOWLFHDH-UHFFFAOYSA-N (2R,2'R,3R,3'R,4R)-3,3',4',5,7-Pentahydroxyflavan(48)-3,3',4',5,7-pentahydroxyflavan Natural products C=12OC(C=3C=C(O)C(O)=CC=3)C(O)CC2=C(O)C=C(O)C=1C(C1=C(O)C=C(O)C=C1O1)C(O)C1C1=CC=C(O)C(O)=C1 XFZJEEAOWLFHDH-UHFFFAOYSA-N 0.000 description 4
- 241000605059 Bacteroidetes Species 0.000 description 4
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 4
- MOJZMWJRUKIQGL-FWCKPOPSSA-N Procyanidin C2 Natural products O[C@@H]1[C@@H](c2cc(O)c(O)cc2)Oc2c([C@H]3[C@H](O)[C@@H](c4cc(O)c(O)cc4)Oc4c3c(O)cc(O)c4)c(O)cc(O)c2[C@@H]1c1c(O)cc(O)c2c1O[C@@H]([C@H](O)C2)c1cc(O)c(O)cc1 MOJZMWJRUKIQGL-FWCKPOPSSA-N 0.000 description 4
- 235000019658 bitter taste Nutrition 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 239000003205 fragrance Substances 0.000 description 4
- HGVVOUNEGQIPMS-UHFFFAOYSA-N procyanidin Chemical compound O1C2=CC(O)=CC(O)=C2C(O)C(O)C1(C=1C=C(O)C(O)=CC=1)OC1CC2=C(O)C=C(O)C=C2OC1C1=CC=C(O)C(O)=C1 HGVVOUNEGQIPMS-UHFFFAOYSA-N 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000001953 sensory effect Effects 0.000 description 4
- 235000021391 short chain fatty acids Nutrition 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 241000194033 Enterococcus Species 0.000 description 3
- 241000588722 Escherichia Species 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 3
- 235000019606 astringent taste Nutrition 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000013325 dietary fiber Nutrition 0.000 description 3
- 208000035475 disorder Diseases 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 235000019614 sour taste Nutrition 0.000 description 3
- 235000019605 sweet taste sensations Nutrition 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 241000425347 Phyla <beetle> Species 0.000 description 2
- 241000605861 Prevotella Species 0.000 description 2
- 244000292604 Salvia columbariae Species 0.000 description 2
- 235000012377 Salvia columbariae var. columbariae Nutrition 0.000 description 2
- 235000001498 Salvia hispanica Nutrition 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 235000014167 chia Nutrition 0.000 description 2
- 229940079919 digestives enzyme preparation Drugs 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 230000007407 health benefit Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 210000002490 intestinal epithelial cell Anatomy 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 229940039696 lactobacillus Drugs 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000011812 mixed powder Substances 0.000 description 2
- 235000015816 nutrient absorption Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- 150000003180 prostaglandins Chemical class 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 241001446247 uncultured actinomycete Species 0.000 description 2
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- PXGPLTODNUVGFL-BRIYLRKRSA-N (E,Z)-(1R,2R,3R,5S)-7-(3,5-Dihydroxy-2-((3S)-(3-hydroxy-1-octenyl))cyclopentyl)-5-heptenoic acid Chemical compound CCCCC[C@H](O)C=C[C@H]1[C@H](O)C[C@H](O)[C@@H]1CC=CCCCC(O)=O PXGPLTODNUVGFL-BRIYLRKRSA-N 0.000 description 1
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 description 1
- 241000589220 Acetobacter Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 206010008570 Chloasma Diseases 0.000 description 1
- 206010008631 Cholera Diseases 0.000 description 1
- 241000928610 Coula Species 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 description 1
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 description 1
- 229920002079 Ellagic acid Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 244000235659 Rubus idaeus Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 229960002852 ellagic acid Drugs 0.000 description 1
- 235000004132 ellagic acid Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 108010093305 exopolygalacturonase Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- -1 flavonoid compounds Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 1
- 229940107187 fructooligosaccharide Drugs 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 1
- 150000003271 galactooligosaccharides Chemical class 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 230000004609 intestinal homeostasis Effects 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000000207 lymphocyte subset Anatomy 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000003821 menstrual periods Effects 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- VWMVAQHMFFZQGD-UHFFFAOYSA-N p-Hydroxybenzyl acetone Natural products CC(=O)CC1=CC=C(O)C=C1 VWMVAQHMFFZQGD-UHFFFAOYSA-N 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- NJGBTKGETPDVIK-UHFFFAOYSA-N raspberry ketone Chemical compound CC(=O)CCC1=CC=C(O)C=C1 NJGBTKGETPDVIK-UHFFFAOYSA-N 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000019722 synbiotics Nutrition 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 229940005605 valeric acid Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 235000021269 warm food Nutrition 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L25/00—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof
- A23L25/20—Food consisting mainly of nutmeat or seeds; Preparation or treatment thereof consisting of whole seeds or seed fragments
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/537—Salvia (sage)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
- A61K36/725—Ziziphus, e.g. jujube
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/78—Saururaceae (Lizard's-tail family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a ganoderma lucidum polysaccharide composition for regulating female intestinal flora and a preparation method thereof, wherein the ganoderma lucidum polysaccharide composition comprises the following raw materials of ganoderma lucidum polysaccharide, procyanidine of sea buckthorn seeds, mulberry leaf extract, houttuynia cordata extract, raspberry fruit powder, red date extract and chia seed powder, and the raw materials are weighed according to the weight part ratio and are uniformly mixed to obtain the ganoderma lucidum polysaccharide composition; wherein the ganoderan consists of 7 monosaccharides including fucose, galactose, glucose, xylose, mannose, glucuronic acid and glucosamine. The ganoderma lucidum polysaccharide composition furthest exerts the effect of regulating female intestinal flora by ganoderma lucidum polysaccharide, and exerts the complementary optimization of each raw material on the effects, aroma and taste by compounding each raw material.
Description
Technical Field
The invention belongs to the technical field of functional foods, and particularly relates to a ganoderan composition for regulating female intestinal flora and a preparation method thereof.
Background
The intestinal tract is the most important nutrient absorption organ of human body, participates in various physiological activities such as food digestion, nutrient absorption, energy supply, essential vitamin generation, immunoregulation, gastrointestinal tract homeostasis and the like, and is closely related to the occurrence and development of various metabolic diseases and inflammations. The intestinal tract is also the largest immune organ of the human body, intestinal epithelial cells also form a mucosal immune barrier besides the digestive metabolic function, and more than 70 percent of immune cells of the human body exist in the intestinal mucosa. A huge number of microbial communities exist in the intestinal tract, are the 'second set of genome' of a human body, and have an important role in establishing an intestinal mucosa immune system, and after the intestinal flora is fixedly planted and perfected, a mycoderm with an important physiological function is formed on the intestinal mucosa. Anaerobic bacteria such as bifidobacterium, lactobacillus and the like are combined with specific receptors on the surface of the intestinal mucosa epithelium and are tightly adhered to the intestinal mucosa epithelium to form a biomembrane structure and a biological barrier with relatively fixed components, so that the intestinal flora colonization resistance is formed, and the invasion of exogenous substances to organisms can be effectively resisted. The intestinal flora and the intestinal mucosa immune system are co-evolved to jointly maintain the intestinal homeostasis. The intestinal flora is closely related to the change of the barrier function of the intestinal tract, and plays an important role in promoting the development of the intestinal mucosa immune system, regulating and controlling the intestinal mucosa immunity, resisting infection and the like. The normal flora plays an important role in regulating the development of various lymphocyte subsets in the intestinal tract, and the interaction of the nutrition and the metabolism of the intestinal tract obviously influences the health and the immune condition of the organism.
The selective administration of prebiotics, probiotics and synbiotics is an important way to maintain the balance of the intestinal flora. Prebiotics are substances that are not utilized by harmful pathogenic bacteria in the intestinal tract, but are utilized in large quantities by probiotics, thereby enhancing the competitive advantage of the probiotics. The concept was first proposed by Gibson in 1995, and then as related research advances and applications expand, gibson, food and agriculture organization of the united nations, etc. revised prebiotic definitions many times. The 2016 international association of probiotics and prebiotics science (ISAPP) expert group, based on the latest scientific and clinical evidence, updated the definition of prebiotics (a substrate that can be selectively utilized by microorganisms and confers health benefits to the host), and products that clearly indicate the ability to be selectively utilized by host microorganisms to produce health benefits are all called prebiotics, meaning that research in this field is entering a new stage of development. At present, the more mature prebiotics are mainly various oligosaccharides including fructo-oligosaccharide, galacto-oligosaccharide, inulin, breast milk oligosaccharide and the like, and certain polysaccharides are regarded as high-quality sources of novel prebiotics due to the comprehensive effects of promoting the proliferation of probiotics, improving the intestinal health of organisms, improving the immunity, preventing tumors and the like, but the research of the novel prebiotics is just started, the interaction research between the prebiotics and the intestinal flora is not deep enough, and the systematicness is not strong.
The source and structure of the polysaccharide have a great influence on the efficacy of the polysaccharide, and the chain length, the type of glycosidic bond, the connection mode and the molecular mass all have influence on the overall efficacy and the regulation effect of intestinal flora. At present, a complete system is not formed in the screening and efficacy evaluation of the probiotics polysaccharide, the product variety of the comprehensive efficacy polysaccharide can not meet the requirement, and the screening of the novel probiotics polysaccharide is in the vogue. Wherein, the ganoderma lucidum is an important traditional Chinese medicinal material in Zhejiang, the content of polysaccharide is high, and the ganoderma lucidum polysaccharide is an important bioactive substance, and the influence of the ganoderma lucidum polysaccharide on the human intestinal flora is not clear.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a ganoderan composition for regulating female intestinal flora and a preparation method thereof, which furthest exert the effect of regulating female intestinal flora by ganoderan and exert the complementary optimization of the effects, aroma and taste of all raw materials by compounding all raw materials.
The purpose of the invention is realized by the following technical scheme:
a ganoderan composition for regulating female intestinal flora comprises ganoderan, fructus Hippophae procyanidine, a mulberry leaf extract, a houttuynia cordata extract, raspberry fruit powder, a red date extract and chia seed powder, and the weight parts of the raw materials are as follows:
weighing the raw materials according to the weight part ratio, and uniformly mixing to obtain the ganoderma lucidum polysaccharide composition.
Further, the method for purifying the ganoderma lucidum polysaccharide comprises the following steps: drying Ganoderma lucidum fruiting body at 40-70 ℃, sieving with a 50-mesh sieve after crushing, soaking for 24h according to 8mL volume times of 95% ethanol of 1g dry powder, removing lipids and small molecular substances, drying filter residues under the above conditions, uniformly mixing according to the proportion of 10-30mL water of 1g powder, carrying out 500w ultrasonic treatment for 30min, carrying out 40-90 ℃ water bath treatment for 60-120min, centrifuging to obtain supernatant, precipitating for 4-8h with 5 volume times of 95% ethanol, centrifuging to collect precipitate, dissolving in deionized water again according to the concentration of 5mg/mL, dialyzing for 72h with a 5000Da bag, passing through a sephadex column to obtain a single component, carrying out vacuum concentration on the obtained eluate, and carrying out freeze drying to obtain purified Ganoderma lucidum polysaccharide.
Further, the ganoderma lucidum polysaccharide is composed of 7 monosaccharides, namely fucose, galactose, glucose, xylose, mannose, glucuronic acid and glucosamine.
Further, the preparation method of the seabuckthorn seed procyanidin comprises the following steps: taking the seabuckthorn seed meal after extracting the seabuckthorn seed oil as a raw material, carrying out flash extraction by using an ethanol solution at a low temperature, and filtering to obtain an extracting solution; recovering ethanol from the extract at low temperature under negative pressure by using a heat pump device, and concentrating the extract to obtain an extract; and (3) the extract is subjected to a belt drying device at a low temperature and under a negative pressure condition to obtain powdery seabuckthorn seed procyanidine.
Further, the preparation method of the mulberry leaf extract comprises the following steps: cutting fresh mulberry leaves into strips, soaking the strips by using an acetic acid solution, filtering, collecting filtrate, centrifuging the obtained filtrate, collecting supernatant, concentrating and drying the supernatant to solid so as to obtain the mulberry leaf extract.
Further, the preparation method of the houttuynia cordata extract comprises the following steps: cutting fresh houttuynia cordata to obtain houttuynia cordata powder, decocting the powder with water under the condition of microwave and ultrasonic wave combined auxiliary dissolution, filtering to obtain houttuynia cordata extract, concentrating the extract under reduced pressure under the vacuum condition to obtain thick paste, and preparing the thick paste into the houttuynia cordata extract by a spray drying machine.
Further, the preparation method of the raspberry fruit powder comprises the following steps: cleaning fresh raspberry fruits without pedicels, crushing to prepare raspberry fruit pulp, adding an enzyme preparation, and performing enzymolysis at room temperature for 1-4h; passing the raspberry pulp after enzymolysis through a homogenizer for 2-3 times to obtain homogenized raspberry pulp; and (3) passing the raspberry slurry through a high-pressure homogenizer to obtain the raspberry slurry after high-pressure homogenization, and performing low-temperature spray drying on the raspberry slurry to obtain raspberry fruit powder.
Further, the preparation method of the red date extract comprises the following steps: taking dry red dates as raw materials, washing, soaking, crushing into red date pieces, adding an enzyme preparation, carrying out enzymolysis for 1-4h at 40-50 ℃, filtering to obtain supernatant, and carrying out vacuum concentration on the supernatant to obtain semi-solid red date paste; and (3) carrying out vacuum drying, crushing and sieving treatment on the semi-solid red date paste to obtain the red date extract.
Further, the preparation method of the chia seed powder comprises the following steps: removing impurities from seeds of chia sold in the market, cleaning, airing, grinding into powder, and sieving with a 200-mesh sieve to obtain the chia seed powder.
Another aspect of the invention:
the application of the ganoderan composition is used for preparing foods, medicines or health-care products for preventing and/or regulating female intestinal flora disorder.
Further, the food, the medicine or the health-care product comprises other acceptable auxiliary materials in the food, the medicine or the health-care product, and the auxiliary materials are one of powder, tablets, granules, capsules or pills.
Another aspect of the invention:
the application of the mixed components of chia seed powder, raspberry fruit powder and red date extract in improving bitterness and fishy taste and covering fishy smell is as follows:
5-10 parts of raspberry fruit powder
5-10 parts of red date extract
3-6 parts of chia seed powder.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, the influence of the ganoderan composition on the composition of intestinal flora and important metabolites thereof of healthy adults is researched, so that the ganoderan composition can reduce the abundance of proteobacteria and increase the relative abundance of bacteroides; and analysis on a subordinate level shows that the addition of the ganoderan composition can reduce the relative abundance of escherichia coli-shigella regardless of men and women and regardless of age groups, and particularly the reduction range of women is greater than that of men; after the ganoderma lucidum polysaccharide composition is added, the abundance of bifidobacteria of women shows an increasing trend, the original abundance of middle-aged women is lower, and the increase amplitude is higher; therefore, the ganoderan composition is suitable for preparing foods, medicines or health-care products for preventing and/or regulating the female intestinal flora disorder;
2. according to the invention, the ganoderma lucidum polysaccharide composition is prepared by selecting several raw materials of ganoderma lucidum polysaccharide, sea-buckthorn seed procyanidine, mulberry leaf extract, houttuynia cordata extract, raspberry fruit powder, red date extract and chia seed powder, and the complementary optimization of the raw materials in terms of efficacy, fragrance and taste is exerted; the mulberry leaf extract and the houttuynia cordata extract have good health-care effects of dispelling wind and heat, clearing away the lung-heat, moistening dryness, resisting bacteria and viruses and repairing intestinal mucosa, but have bitter and astringent tastes and fishy smells, the application of the mulberry leaf extract and the houttuynia cordata extract in foods or health-care products is greatly influenced, the raspberry fruit powder and the red date extract have special sour and sweet tastes and strong fragrance, the chia seed powder is smooth in taste, rich in dietary fibers and capable of moistening intestines and expelling toxins, the bitter and astringent tastes and the fishy smells of the mulberry leaf extract and the houttuynia cordata extract can be wrapped and passivated, the bitter and fishy smells of the mulberry leaf extract and the houttuynia cordata extract are well improved after mixing, the fishy smells are covered, and meanwhile, the sea buckthorn seed procyanidin and the raspberry fruit powder are rich in phytocyanidin, and the effects of resisting oxidation and cleaning blood are added to the composite product;
3. in the ganoderma lucidum polysaccharide composition, the mulberry leaf extract and the houttuynia cordata extract are added, so that the effects of diminishing inflammation and repairing intestinal mucosa are exerted, and the beneficial effects of the ganoderma lucidum polysaccharide in regulating intestinal flora disorder and repairing intestinal cell damage are assisted and enhanced;
4. from the aspect of food supplement of traditional Chinese medicine, the red date extract and chia seed powder in the ganoderma lucidum polysaccharide composition belong to warm food, the addition of the red date extract and chia seed powder can neutralize the cold property of the mulberry leaf extract and the houttuynia cordata extract, and the composition is smooth in nature after the raw materials are combined, and is suitable for women to drink all the year round; meanwhile, the chia seed powder is rich in dietary fiber, vitamins and polyunsaturated fatty acid, is matched with natural antioxidant seabuckthorn seed procyanidin, can obviously reduce the generation of prostaglandin PGF2 alpha and prostaglandin LT4 and free radicals which cause female dysmenorrheal, is suitable for being eaten by women during or before menstrual period, and has the function of relieving dysmenorrheal.
Drawings
The invention is further illustrated by the following examples in conjunction with the accompanying drawings:
FIG. 1 is a graph of total sugar standards;
FIG. 2 is a diagram of the molecular weight distribution of ganoderan;
FIG. 3 is a HPLC chart of the standard, wherein the left is other monosaccharides and the right is glucosamine;
FIG. 4 is a graph showing the results of chao1 and shannon index;
FIG. 5 is a graph of PCA for different population combinations;
FIG. 6 is a graph of the results of the effect of ganoderan compositions on gut flora composition (phylum level);
FIG. 7 is a graph showing the effect of ganoderan composition on the composition of intestinal flora (genus level);
FIG. 8 is a graph showing the effect of ganoderan composition on the production of short chain fatty acids by intestinal flora;
FIG. 9 is a graph showing the effect of ganoderan composition on gas production by gut flora.
Detailed Description
Example 1 ganoderan compositions and preparation thereof
The embodiment provides a ganoderan composition for regulating female intestinal flora, which consists of ganoderan, seabuckthorn seed procyanidine, a mulberry leaf extract, a houttuynia cordata extract, raspberry fruit powder, a red date extract and chia seed powder, wherein the ganoderan composition comprises the following raw materials in parts by weight:
weighing the raw materials according to the weight part ratio, and uniformly mixing to obtain the ganoderma lucidum polysaccharide composition which is mixed powder. When the ganoderma lucidum polysaccharide composition is eaten, a proper amount of ganoderma lucidum polysaccharide composition is taken, warm water is added, and the ganoderma lucidum polysaccharide composition is stirred and dissolved, so that the ganoderma lucidum polysaccharide composition can be eaten.
The method for purifying the ganoderma lucidum polysaccharide comprises the following steps: drying Ganoderma lucidum fruiting body at 40-70 deg.C, pulverizing, sieving with 50 mesh sieve, soaking in 8mL volume times of 95% ethanol for 24h according to 1g dry powder, removing lipid and small molecular substances, drying the filter residue under the above conditions, mixing uniformly according to the proportion of 10-30mL water of 1g powder, performing 500w ultrasonic treatment for 30min, performing water bath treatment at 40-90 deg.C for 60-120min, centrifuging to obtain supernatant, precipitating with 5 volume times of 95% ethanol for 4-8h, centrifuging to collect precipitate, dissolving in deionized water again according to 5mg/mL concentration, dialyzing with 5000Da bag for 72h, passing through Sephadex column to obtain single component, vacuum concentrating the obtained eluate, and freeze drying to obtain purified Ganoderma lucidum polysaccharide.
The total sugar and the reducing sugar are measured by two methods, namely an absorbance method and an enzyme-labeling method, and an intermediate value is taken. The absorbance method and the enzyme-linked immunosorbent assay are both used for determining the purity of the ganoderma lucidum polysaccharide, and the purity is 75%. The total sugar standard curve is shown in figure 1. The molecular weight of ganoderan was determined by HPLC method, and is shown in FIG. 2 and Table 1.
TABLE 1 molecular weight information of ganoderan
As can be seen from FIG. 2 and Table 1, the weight average molecular weight of ganoderan is lower, 21043. The monosaccharide composition is determined by ion chromatography, 11 monosaccharides of fucose, arabinose, rhamnose, galactose, glucose, xylose, fructose, mannose, galacturonic acid, glucuronic acid and glucosamine are used as standard control, and HPLC chart and information are shown in FIG. 3 and Table 2.
TABLE 2 Standard substance types and related information (concentration 5 mg/L)
TABLE 3 monosaccharide composition of ganoderan
As can be seen from table 3, the ganoderan has a relatively complex composition, and consists of 7 monosaccharides, i.e., fucose, galactose, glucose, xylose, mannose, glucuronic acid, and glucosamine, wherein the glucose content is the highest and is 67%, the glucuronic acid content is lower and is about 3.4%, and the content of the remaining 5 monosaccharides is not much different and is between 5 and 7%, which increases many difficulties for the analysis of the fine structure.
The preparation method of the seabuckthorn seed procyanidin comprises the following steps: taking semen Hippophae dregs after extracting oleum Hippophae as raw material, extracting with 60% ethanol solution at low temperature of 15 deg.C for 2-3 times with flash extraction for 10min each time, mixing 2-3 times of extractive solutions, and filtering with 300 mesh filter cloth to obtain extractive solution; recovering ethanol from the filtered extractive solution at low temperature of 15 deg.C and negative pressure of-0.098 MPa by heat pump device, and concentrating the extractive solution to obtain extract; and (3) the extract is processed by a belt type drying device at the low temperature of 15 ℃ and under the negative pressure of-0.098 MPa to obtain the powdery seabuckthorn seed procyanidine.
The preparation method of the mulberry leaf extract comprises the following steps: cutting fresh mulberry leaves into strips with the width of 1cm, soaking for 1-3h at the temperature of 45 ℃ by using an acetic acid solution with the concentration of 0.05M, filtering by using gauze, collecting filtrate, centrifuging the obtained filtrate for 10-20min at 3000r/M, collecting supernatant, concentrating and drying to solid, thereby obtaining the mulberry leaf extract.
The preparation method of the houttuynia cordata extract comprises the following steps: cutting fresh houttuynia cordata to obtain houttuynia cordata powder, decocting the powder with water for 2 to 4 hours under the condition of microwave (power of 400 to 500W) and ultrasonic (power of 600 to 750W) combined auxiliary dissolution, filtering to obtain houttuynia cordata extract, concentrating the extract under reduced pressure under the vacuum condition (vacuum degree of 0.06MPa to 0.08 MPa) to form thick paste, and preparing the houttuynia cordata extract from the thick paste through a spray drying machine.
The preparation method of the raspberry fruit powder comprises the following steps: cleaning fresh raspberry fruits without pedicels, crushing to prepare raspberry fruit pulp, adding an enzyme preparation, and performing enzymolysis at room temperature for 1-4h; passing the enzyme-hydrolyzed raspberry pulp through a homogenizer for 2-3 times to obtain homogenized raspberry pulp; and (4) passing the raspberry slurry through a high-pressure homogenizer to obtain the raspberry slurry after high-pressure homogenization, and carrying out low-temperature spray drying on the raspberry slurry to obtain the raspberry fruit powder.
The raspberry has full fruits and ruddy color, can be picked directly for eating, tastes sour and sweet, and has higher nutritional value. The raspberry contains various functional components such as flavonoid compounds, ellagic acid, anthocyanin, salicylic acid, raspberry ketone and the like besides abundant amino acids, minerals and vitamins; has various biological effects of removing chloasma, improving dysmnesia, treating osteoporosis, treating gynecological diseases and the like, and has the reputation of life fruit. The raspberry fruit powder obtained after enzymolysis has special sour and sweet taste and strong fragrance.
The preparation method of the red date extract comprises the following steps: taking dry red dates as raw materials, washing, soaking, crushing into red date pieces, adding an enzyme preparation, carrying out enzymolysis for 1-4h at 40-50 ℃, filtering to obtain supernatant, and carrying out vacuum concentration on the supernatant to obtain semi-solid red date paste; and (3) carrying out vacuum drying, crushing and sieving treatment on the semi-solid red date paste to obtain the red date extract.
In the preparation of the raspberry fruit powder and the red date extract, the enzyme preparations are mixed enzyme preparations of cellulase and pectinase.
The preparation method of the chia seed powder comprises the following steps: removing impurities from seeds of chia sold in the market, cleaning, airing, grinding into powder, and sieving with a 200-mesh sieve to obtain the chia seed powder.
Comparative examples 1 to 5
The comparative example provides a ganoderan composition, and the components and parts by weight of the raw materials are shown in the following table 4:
TABLE 4 COMPARATIVE EXAMPLES 1-5 TABLE FOR COMPARATIVE EXAMPLES OF LINGZHITAN POLYSACCHARIDE COMPOSITIONS
| Raw materials | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 |
| Ganoderma lucidum polysaccharide | 45 | 45 | 45 | 45 | 45 |
| Mulberry LeavesExtract of plant | 6 | 6 | 6 | 6 | 6 |
| Houttuynia cordata extract | 3.5 | 3.5 | 3.5 | 3.5 | 3.5 |
| Sea-buckthorn seed procyanidine | 7 | 7 | 7 | 7 | 7 |
| Chia seed powder | / | 24 | / | / | 4.5 |
| Raspberry fruit powder | / | / | 24 | / | 19.5 |
| Red date extract | / | / | / | 24 | / |
Weighing the raw materials according to the weight part ratio in the table above, and uniformly mixing to obtain the ganoderma lucidum polysaccharide composition which is mixed powder. When the ganoderma lucidum polysaccharide composition is eaten, a proper amount of ganoderma lucidum polysaccharide composition is taken, warm water is added, and the ganoderma lucidum polysaccharide composition is stirred and dissolved, so that the ganoderma lucidum polysaccharide composition can be eaten.
The preparation of each starting material was as described in example 1.
The trained personnel perform sensory evaluation on the ganoderan composition described in example 1 and comparative examples 1-5, wherein the fishy smell evaluation score of comparative example 1 (without chia seed powder, raspberry fruit powder and red date extract added) is set to 10.
The sensory evaluation results of the examples and comparative examples are shown in table 5. The results show that the three mixed components of chia seed powder, raspberry fruit powder and red date extract have better regulating effect on the fishy smell than the combination of any one or two of the components. The raspberry fruit powder and the red date extract have special sour and sweet taste and strong fragrance, the chia seed powder is soft and smooth in taste, is rich in dietary fiber, has the effects of moistening intestines and expelling toxin, and can wrap and inactivate bad taste including bitter and astringent taste and fishy smell.
TABLE 5 sensory evaluation results of ganoderan compositions
| Numbering | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 | Example 1 |
| Sensory evaluation score | 10 | 8.0 | 6.5 | 6.0 | 6.5 | 2.0 |
Example 2 Regulation of the intestinal flora by a ganoderan composition
32 healthy adults were divided into four groups, namely 20-30 years old, named YM (yang male), YF (yang male), 40-50 years old, named MM (middle male), MF (middle male), GC (control group) without ganoderan composition, LZ (lingzhi) with ganoderan composition (5 g/L), and GC-YM, LZ-YF, GC-MM, and LZ-MF.
1. Influence on diversity
The Chao1 index is an index for measuring the abundance of a species, and is independent of abundance and uniformity, but is sensitive to rare species. The Shannon index is related to the number and uniformity of individual distribution (uniformity or evenness) among the populations, and the more uniform the individual distribution among the populations, the greater the Shannon index. The diversity index is maximal if each individual belongs to a different species; if each individual belongs to the same species, its diversity index is minimal. The larger the shannon index, the better the sample homogeneity is reflected. As can be seen from FIG. 4, the ganoderan composition has no significant effect on the abundance and diversity of human intestinal flora; the flora abundance of middle-aged men is increased; the flora diversity of women is slightly reduced.
PCA (Principal Components Analysis) is used to characterize beta-diversity, a projection technique that refers to mapping data in a high-dimensional space to a low-dimensional space. For example, a sphere in a three-dimensional space is projected in the coordinate axis direction to form a circle, and in the projection process of changing the sphere into the circle, part of the properties of the original object are lost and retained, so that the method is helpful for reducing multi-dimensional information into two-dimensional information and visually understanding data.
As can be seen from fig. 5, the ganoderan composition has no significant effect on the composition of male and female flora, i.e. beta-diversity, regardless of the young and middle aged groups, indicating that it has no significant effect on the stability of the intestinal flora of healthy people and has potential for application as a daily health food.
2. Influence on changes in intestinal flora
The human intestinal flora mainly comprises actinomycetomycota, bacteroidetes, firmicutes, proteobacteria and the like, wherein the bacteroidetes generally refers to bacteria which grow in human intestinal tracts and stomachs and play a positive role in human health, the abundance of the bacteroides is generally increased to be beneficial to the human health, some potential probiotics, namely the bacteroides parapsilosis also belong to the phylum at present, and a large amount of bacteroides in fecal intestinal flora of some pathogenic bacteria infected patients is reduced or even disappears. The phylum firmicutes includes Bacillus, enterococcus, lactobacillus, lactococcus, leuconostoc, acetobacter, etc., including many probiotic strains; furthermore, the higher the ratio of firmicutes to bacteroides, the more efficient the absorption of nutrients in food, and conversely the potential for weight loss. Proteobacteria comprise various pathogenic bacteria, such as escherichia coli, salmonella, cholera navy, helicobacter pylori and the like, the microecological imbalance process during metabolic disorder is accompanied by the increase of the Proteobacteria, and the dominant Proteobacteria also exist in the intestinal tracts of children with malnutrition, so that immune imbalance and inflammation are caused, and researchers think that the Proteobacteria is a potential identification standard for occurrence of microecological imbalance and diseases.
As can be seen from fig. 6, in the scope of the present experiment, from the phylum level, after the ganoderan composition described in example 1 is added, the relative abundance of bacteroides is significantly increased in the case of both men and women, and the abundance of proteobacteria is significantly decreased. The relative abundance of male firmicutes is not large in change range, the relative abundance of the firmicutes of young women is slightly increased, the relative abundance of the firmicutes of middle-aged women is higher, and the abundance is slightly reduced after the ganoderma lucidum polysaccharide composition is added. The relative abundance of actinomycete phyla in males has a descending trend; the relative abundance of actinomycete phyla in women has little change, and the middle-aged group has slightly increased.
As can be seen from fig. 7, analysis at a subordinate level, addition of the ganoderan composition described in example 1 reduced the relative abundance of escherichia coli-shigella regardless of male and female, but the reduction was greater in female than male; the relative abundance of the four bacteroidetes is obviously increased, and the increase range of the middle-aged people is larger than that of the young people; the abundance of bifidobacteria in young men is increased, the original relative abundance of bifidobacteria in middle-aged men is higher, the relative abundance of bifidobacteria in women is increased when the ganoderan described in example 1 is added, and the original abundance of bifidobacteria in middle-aged women is lower and the increase amplitude is higher; four groups of Prevotella, coorales and enterococcus showed a remarkable increase. The ganoderma lucidum polysaccharide of example 1 can regulate the intestinal flora structure of human body and develop towards a healthier direction.
3. Influence on short-chain fatty acid production by intestinal flora
This example separately investigated the effect of the ganoderan composition described in example 1 on the metabolism of the gut flora to produce acetic, propionic, butyric, isobutyric, valeric, isovaleric 6 short chain fatty acids and total acids. It was found that the ganoderan composition of example 1 significantly promotes the production of acetic acid in both male and female groups, both young and middle age groups; the trend of propionic acid and butyric acid is also the same, and the trend is obviously increased; valeric acid changes were insignificant and total short chain fatty acid content was significantly increased. Therefore, the ganoderan composition in example 1 can obviously increase the content of short-chain fatty acids in the intestinal tract, and has important significance in reducing the pH value of the intestinal tract, improving the composition of the intestinal flora and maintaining a healthy environment.
4. Influence on gas production of intestinal flora
This example investigated the metabolism of intestinal flora by CH produced by the ganoderan composition described in example 1 4 、NH 3 、H 2 S、H 2 、CO 2 The influence of five gases. It was found that the ganoderan composition described in example 1 was able to significantly reduce CH in 4 groups 4 Yield, H of young male group 2 S content also tends to decrease, excessive CH 4 And H 2 S has adverse effects on the composition of intestinal epithelial cells and flora; NH 3 The content of (A) is not changed greatly and has an ascending trend; h 2 With CO 2 The two neutral gas contents are obviously increased, and probably are related to the improvement of the expected flora composition, and the influence and the principle of the two neutral gas contents need to be further studied deeply.
And (4) conclusion:
the ganoderma lucidum polysaccharide is further purified in the embodiment 1 of the invention, and the purity reaches 75%; the average molecular weight of the polysaccharide is 21KD, the composition of the polysaccharide is relatively complex, the polysaccharide consists of 7 monosaccharides including fucose, galactose, glucose, xylose, mannose, glucuronic acid and glucosamine, wherein the glucose content is the highest and accounts for 67%, the glucuronic acid content is lower and accounts for about 3.4%, and the content of the other 5 monosaccharides is not greatly different and is between 5 and 7%.
Example 2 the effect of the composition of ganoderan according to example 1 on the composition of the intestinal flora and the composition of important metabolites thereof (8 people are group 1, and are classified according to age and gender, and are respectively GC-YM, LZ-YF, GC-MM and LZ-MF groups) of 32 healthy adults was studied, and from the phylum level, the addition of the ganoderan composition according to example 1 can reduce the abundance of proteobacteria and increase the relative abundance of Bacteroides, with slightly different changes in male and female; analysis on the subordinate level, addition of the ganoderan composition described in example 1 was able to reduce the relative abundance of escherichia coli-shigella, both in men and in women, but the reduction was greater in women than in men; the relative abundance of 4 groups of bacteroidetes is obviously increased, and the increase range of middle-aged people is larger than that of young people; the abundance of bifidobacteria in young men is increased, the original relative abundance of bifidobacteria in middle-aged men is higher, the relative abundance of bifidobacteria is reduced after the ganoderan composition in example 1 is added, the abundance of bifidobacteria in women tends to increase, and the original abundance of bifidobacteria in middle-aged women is lower and has higher increase amplitude; four groups of Prevotella, coula and enterococcus showed a remarkable increase.
The ganoderan composition in example 1 has a relatively obvious effect of promoting the generation of important short-chain fatty acids such as acetic acid, propionic acid and butyric acid, and is suitable for male or female, young or middle-aged people; the total short chain fatty acid content is obviously increased. The ganoderan composition described in example 1 can significantly reduce CH 4 、H 2 Production of harmful gases such as S, but to H 2 With CO 2 The increase in the content of the two neutral gases has a promoting effect, which may be related to the improvement of the flora composition thereof, for reasons to be further investigated.
Finally, it should be noted that the above only illustrates the technical solution of the present invention, but not limited thereto, and although the present invention has been described in detail with reference to the preferred arrangement, it will be understood by those skilled in the art that modifications or equivalent substitutions may be made thereto without departing from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. The ganoderma lucidum polysaccharide composition for regulating female intestinal flora is characterized by comprising the following raw materials in parts by weight:
ganoderma polysaccharide 25-50
Fructus Hippophae procyanidine 5-10
5-10 of mulberry leaf extract
Herba Houttuyniae extract 3-6
5-10 parts of raspberry fruit powder
5-10 of red date extract
3-6 parts of chia seed powder;
weighing the raw materials according to the weight part ratio, and uniformly mixing to obtain the ganoderma lucidum polysaccharide composition.
2. The ganoderan composition for regulating female intestinal flora according to claim 1, wherein the ganoderan composition comprises the following raw materials in parts by weight:
ganoderma lucidum polysaccharide 30-40
Fructus Hippophae procyanidine 6-8
5-7 parts of mulberry leaf extract
Herba Houttuyniae extract 3-4
8-10 parts of raspberry fruit powder
6-9 parts of red date extract
4-5 parts of chia seed powder.
3. The ganoderan composition for regulating female intestinal flora according to claim 1 or 2, characterized in that the ganoderan is extracted and purified by the method comprising: drying Ganoderma lucidum fruiting body at 40-70 deg.C, pulverizing, sieving with 50 mesh sieve, soaking in 8mL volume times of 95% ethanol for 24h according to 1g dry powder, removing lipid and small molecular substances, drying the filter residue under the above conditions, mixing uniformly according to the proportion of 10-30mL water of 1g powder, performing 500w ultrasonic treatment for 30min, performing water bath treatment at 40-90 deg.C for 60-120min, centrifuging to obtain supernatant, precipitating with 5 volume times of 95% ethanol for 4-8h, centrifuging to collect precipitate, dissolving in deionized water again according to 5mg/mL concentration, dialyzing with 5000Da bag for 72h, passing through Sephadex column to obtain single component, vacuum concentrating the obtained eluate, and freeze drying to obtain purified Ganoderma lucidum polysaccharide.
4. The ganoderan composition for modulating female gut flora as claimed in claim 1 or 2, wherein the seabuckthorn procyanidins are prepared by: taking the seabuckthorn seed meal after extracting the seabuckthorn seed oil as a raw material, carrying out flash extraction by using an ethanol solution at a low temperature, and filtering to obtain an extracting solution; recovering ethanol from the extract at low temperature and under negative pressure by using a heat pump device, and concentrating the extract to obtain extract; and (3) the extract is subjected to a belt drying device at a low temperature and under a negative pressure condition to obtain powdery seabuckthorn seed procyanidine.
5. The ganoderan composition for modulating female gut flora according to claim 1 or 2, wherein the mulberry leaf extract is prepared by the method comprising: cutting fresh mulberry leaves into strips, soaking the strips in acetic acid solution, filtering, collecting filtrate, centrifuging the obtained filtrate, collecting supernatant, concentrating, and drying to obtain solid to obtain the mulberry leaf extract.
6. The ganoderan composition for modulating the female gut flora of claim 1 or 2, wherein the houttuynia cordata extract is prepared by the method comprising: cutting fresh houttuynia cordata to obtain houttuynia cordata powder, decocting the powder with water under the condition of microwave and ultrasonic wave combined auxiliary dissolution, filtering to obtain houttuynia cordata extract, concentrating the extract under reduced pressure under the vacuum condition to obtain thick paste, and preparing the thick paste into the houttuynia cordata extract by a spray drying machine.
7. The ganoderan composition for regulating female intestinal flora according to claim 1 or 2, characterized in that the raspberry fruit powder is prepared by: cleaning fresh raspberry fruits without pedicels, crushing to prepare raspberry pulp, adding enzyme preparation, and performing enzymolysis at room temperature for 1-4h; passing the enzyme-hydrolyzed raspberry pulp through a homogenizer for 2-3 times to obtain homogenized raspberry pulp; and (3) passing the raspberry slurry through a high-pressure homogenizer to obtain the raspberry slurry after high-pressure homogenization, and carrying out low-temperature spray drying on the raspberry slurry to obtain the raspberry fruit powder.
8. The ganoderan composition for regulating female intestinal flora according to claim 1 or 2, characterized in that the preparation method of the red date extract comprises: taking dry red dates as raw materials, cleaning, soaking, crushing into red date pieces, adding an enzyme preparation, performing enzymolysis for 1-4 hours at 40-50 ℃, filtering to obtain supernatant, and performing vacuum concentration on the supernatant to obtain semi-solid red date paste; and (3) carrying out vacuum drying, crushing and sieving treatment on the semi-solid red date paste to obtain the red date extract.
9. Use of the ganoderan composition according to any one of claims 1 to 8 for the preparation of a food, a pharmaceutical or a nutraceutical for the prevention and/or regulation of disorders of the female intestinal flora.
10. The use of the ganoderan composition according to claim 9, wherein the food, drug or health product comprises other acceptable adjuvants in food, drug or health product, and is one of powder, tablet, granule, capsule or pill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210935355.4A CN115281344A (en) | 2022-08-05 | 2022-08-05 | Ganoderma lucidum polysaccharide composition for regulating female intestinal flora and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210935355.4A CN115281344A (en) | 2022-08-05 | 2022-08-05 | Ganoderma lucidum polysaccharide composition for regulating female intestinal flora and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115281344A true CN115281344A (en) | 2022-11-04 |
Family
ID=83827688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210935355.4A Pending CN115281344A (en) | 2022-08-05 | 2022-08-05 | Ganoderma lucidum polysaccharide composition for regulating female intestinal flora and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115281344A (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1075995A (en) * | 1996-09-04 | 1998-03-24 | Lotte Co Ltd | Deodorant |
| KR20010069504A (en) * | 2001-04-06 | 2001-07-25 | 서정심 | The manufacture way of jujubee honey water |
| CN103271157A (en) * | 2013-05-23 | 2013-09-04 | 宁夏夏进乳业集团股份有限公司 | Health preserving cereal milk and preparation method thereof |
| CN104522806A (en) * | 2014-12-24 | 2015-04-22 | 百花医药集团股份有限公司 | Herba houttuyniae healthcare beverage and preparation method thereof |
| CN104910287A (en) * | 2015-06-04 | 2015-09-16 | 海南科技职业学院 | Extracting method of ganoderan |
| CN105440003A (en) * | 2016-01-12 | 2016-03-30 | 北京宝得瑞健康产业有限公司 | Method for efficiently extracting procyanidine in sea-buckthorn seeds at low temperature and with low energy consumption |
| CN105707771A (en) * | 2016-03-01 | 2016-06-29 | 河北至高点农业科技有限公司 | Dry raspberry powder and preparing method thereof |
| CN105709031A (en) * | 2016-03-29 | 2016-06-29 | 福建农林大学 | Ganoderan complex for treating hand-foot-and-mouth disease and preparation method of ganoderan complex |
| CN106617079A (en) * | 2015-10-30 | 2017-05-10 | 无限极（中国）有限公司 | Purpose of ganoderan in preparation of health food for regulating flora in intestinal tract |
| CN107136509A (en) * | 2017-07-06 | 2017-09-08 | 北京爱博优营养科技有限公司 | It is a kind of to be used to improve functional food of immunity and preparation method thereof |
| CN107927750A (en) * | 2017-12-01 | 2018-04-20 | 深圳福山生物科技有限公司 | Donkey-hide gelatin composition purposes |
| CN112300058A (en) * | 2020-12-03 | 2021-02-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for extracting 1-deoxynojirimycin from fresh mulberry leaves |
| CN113508837A (en) * | 2020-04-10 | 2021-10-19 | 内蒙古蒙牛乳业（集团）股份有限公司 | Lactobacillus beverage containing naked algae powder and chia seed powder and preparation method thereof |
-
2022
- 2022-08-05 CN CN202210935355.4A patent/CN115281344A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1075995A (en) * | 1996-09-04 | 1998-03-24 | Lotte Co Ltd | Deodorant |
| KR20010069504A (en) * | 2001-04-06 | 2001-07-25 | 서정심 | The manufacture way of jujubee honey water |
| CN103271157A (en) * | 2013-05-23 | 2013-09-04 | 宁夏夏进乳业集团股份有限公司 | Health preserving cereal milk and preparation method thereof |
| CN104522806A (en) * | 2014-12-24 | 2015-04-22 | 百花医药集团股份有限公司 | Herba houttuyniae healthcare beverage and preparation method thereof |
| CN104910287A (en) * | 2015-06-04 | 2015-09-16 | 海南科技职业学院 | Extracting method of ganoderan |
| CN106617079A (en) * | 2015-10-30 | 2017-05-10 | 无限极（中国）有限公司 | Purpose of ganoderan in preparation of health food for regulating flora in intestinal tract |
| CN105440003A (en) * | 2016-01-12 | 2016-03-30 | 北京宝得瑞健康产业有限公司 | Method for efficiently extracting procyanidine in sea-buckthorn seeds at low temperature and with low energy consumption |
| CN105707771A (en) * | 2016-03-01 | 2016-06-29 | 河北至高点农业科技有限公司 | Dry raspberry powder and preparing method thereof |
| CN105709031A (en) * | 2016-03-29 | 2016-06-29 | 福建农林大学 | Ganoderan complex for treating hand-foot-and-mouth disease and preparation method of ganoderan complex |
| CN107136509A (en) * | 2017-07-06 | 2017-09-08 | 北京爱博优营养科技有限公司 | It is a kind of to be used to improve functional food of immunity and preparation method thereof |
| CN107927750A (en) * | 2017-12-01 | 2018-04-20 | 深圳福山生物科技有限公司 | Donkey-hide gelatin composition purposes |
| CN113508837A (en) * | 2020-04-10 | 2021-10-19 | 内蒙古蒙牛乳业（集团）股份有限公司 | Lactobacillus beverage containing naked algae powder and chia seed powder and preparation method thereof |
| CN112300058A (en) * | 2020-12-03 | 2021-02-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for extracting 1-deoxynojirimycin from fresh mulberry leaves |
Non-Patent Citations (3)
| Title |
|---|
| 展俊岭等: "鱼腥草总黄酮超声-微波辅助提取及其抗氧化活性", 热带作物学报, vol. 39, no. 10, pages 2067 - 2073 * |
| 张奉春等: "《张奉春降尿酸防痛风饮食》", 中国轻工业出版社, pages: 122 * |
| 张鸿等: "桑提取物对肠道细菌的影响初探", 广东蚕业, vol. 51, no. 12, pages 170 - 171 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104643250B (en) | It is a kind of can bidirectional modulation body weight improve inferior health solid beverage | |
| US11376292B2 (en) | Microbial fermentation composition subjected to enzymolysis, microbial fermentation and microbial transformation and use thereof | |
| KR101339706B1 (en) | A compound for immune strengthen inclusion reducing the bitterness of red ginseng, the extract of immune, and the probiotics | |
| CN105358168B (en) | Composition having function of relieving premenstrual syndrome and dysmenorrhea | |
| KR20100127420A (en) | Composition for improving bioavailability of saponin | |
| CN105142424A (en) | Cereal containing hericium erinaceum and method for making same | |
| CN104872775B (en) | A kind of compound fat-reducing solid beverage of green tea tea polysaccharide and application | |
| CN115777931A (en) | Dietary supplement for coordinating intestines and stomach and preparation method and application thereof | |
| CN111357891A (en) | Plant beverage containing artichoke and ginseng and preparation method thereof | |
| CN100467592C (en) | Preparation of highland barley SOD an conditioning food containing highland barley SOD | |
| KR102167812B1 (en) | Fermented product of sorghum-adzuki bean mixture for intestinal health and improvement effect of bowel | |
| CN114366826A (en) | Preparation method of larch arabinogalactan composition and sterilization treatment device thereof | |
| CN101797049B (en) | Nutrient eight-treasure soup formula and preparation process thereof | |
| CN109820123A (en) | A kind of plant beverage and preparation method thereof improving microcirculation | |
| CN115281344A (en) | Ganoderma lucidum polysaccharide composition for regulating female intestinal flora and preparation method thereof | |
| CN1943448A (en) | Bamboo juice and ginkgo drink and its producing method | |
| KR20030080296A (en) | Composition containing saponin fraction and derivatives isolated from ginseng radix for preventing and treating allergy-mediated disease | |
| CN106234896A (en) | A kind of health promoting beverage treating nephropathy and preparation method thereof | |
| KR20200074061A (en) | Composition for improving cognitive function | |
| CN107467654B (en) | Kelp and sea cucumber compound extract and preparation method and application thereof | |
| CN102640931B (en) | Konjac health-protection consumptive-thirst capsule and preparation method thereof | |
| KR100533505B1 (en) | Health supplement food containing saponin derivatives isolated from ginseng radix for preventing and treating allergy-mediated disease | |
| CN111265627B (en) | Sea cucumber polysaccharide composition for regulating intestinal flora and preparation method and application thereof | |
| CN115708845B (en) | Extraction method of rehmannia root extract and application of rehmannia root extract in preparation of anti-inflammatory drugs | |
| CN114098069B (en) | Lactic acid bacteria combined fermentation product and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |