CN115251047B - Ecological box for storing cell-based biological samples - Google Patents
Ecological box for storing cell-based biological samples Download PDFInfo
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- CN115251047B CN115251047B CN202211015681.XA CN202211015681A CN115251047B CN 115251047 B CN115251047 B CN 115251047B CN 202211015681 A CN202211015681 A CN 202211015681A CN 115251047 B CN115251047 B CN 115251047B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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Abstract
The invention discloses an ecological box for storing cell-based biological samples, which relates to the technical field of ecological boxes and comprises a bottom plate and further comprises: the storage box is fixedly arranged on one side of the bottom plate, a storage groove is formed in the storage box, and one side of the storage box is fixedly connected with the top plate; the storage mechanism arranged in the storage tank comprises a bearing roller, wherein the bearing roller and the storage tank are movably arranged in a matched mode, a limit groove is formed in one side of the bearing roller, an air guide assembly is arranged between the limit groove and the bulge groove, a sealing assembly is arranged at one end of the bearing roller, an installation groove is formed in one end of the storage tank, a carbon dioxide storage tank and an oxygen tank are fixedly arranged on a bottom plate on two sides of the air heater respectively, the two end plates are communicated with the air heater, the air heater is communicated with the air guide assembly, a detection assembly is arranged on the side wall of the storage tank, the quick conversion of environment simulation and sealing storage modes can be automatically realized, and independent environment simulation tests can be realized.
Description
Technical Field
The invention relates to the technical field of ecological boxes, in particular to an ecological box for storing cell-based biological samples.
Background
Cellular organisms refer to all organisms having a cellular structure, which are divided into two classes according to whether or not the cells constituting the organism are nuclear coated with a nuclear membrane: prokaryotes composed of prokaryotes and eukaryotes composed of eukaryotes (not a method of classifying in biology), existing cell-biological tissue samples are generally stored by cold storage at low temperature after being sampled, but when research such as growth laws is needed, cultivation in nutrient solution is needed.
The traditional storage box generally needs ultralow temperature refrigeration, the cell organisms are kept active in a low-temperature environment by the mode, then transported, and tested in a proper environment, but the mode has higher energy consumption, and the long-time transportation cannot maintain a better ecological environment when the low-temperature environment cannot be provided, so that the cell organisms are influenced in activity, and loss is caused.
Disclosure of Invention
The invention provides an ecological box for storing a cell-based biological sample, which solves the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
an ecological box for cell-based biological sample storage, comprising a bottom plate, and further comprising:
the storage box is fixedly arranged on one side of the bottom plate, a storage groove is formed in the storage box, and one side of the storage box is fixedly connected with the top plate; the storage mechanism arranged in the storage tank comprises a bearing roller, wherein the bearing roller and the storage tank are movably arranged in a matched mode, a limit groove is formed in one side of the bearing roller, a rubber sleeve is fixedly connected to one side of the limit groove, a test tube is movably arranged in the limit groove on one side of the rubber sleeve, one end of the test tube is sleeved with a sealing sleeve, two ends of the sealing sleeve are respectively provided with a communicating groove, a reset piece is fixedly connected to one side of the sealing sleeve between the communicating grooves, a valve block is fixedly connected to one side of the reset piece, one end of the valve block is provided with an air guide groove, the air guide groove is of an I-shaped structure, the valve block is in sliding connection with the sealing sleeve, one end of the valve block is rotationally connected with a roller, a bulge groove is formed in the storage tank on one side of the storage tank, an air guide component is arranged between the bulge groove, one end of the bearing roller is provided with a sealing component, an installation groove is formed in one end of the storage tank, a hot air heater is fixedly connected with a bottom plate, a carbon dioxide storage tank and an oxygen tank are respectively arranged on the bottom plate on two sides of the hot air heater, and are communicated with the hot air heater, the hot air heater and the air guide component is communicated with the air guide component, the air guide component is arranged, and the side wall of the storage tank is provided with a detection component under a storage environment, and the storage tank can automatically drive the bearing roller to rotate to realize the automatic simulation and the environment, so that the test can realize the test and the storage of the storage mechanism can realize the test environment can be realized.
As a preferable technical scheme of the invention, the air guide assembly comprises an air guide pipe fixedly arranged in the storage box, two sides of the air guide pipe are fixedly connected with the transfer pipe, one end of the transfer pipe is fixedly connected with the electronic valve, the air outlet end of the electronic valve is communicated with the convex groove, and the electronic valve can flexibly control the conveying and sealing of the air transmitted from the transfer pipe.
As a preferable technical scheme of the invention, the sealing assembly comprises a sealing cover fixedly arranged on one side of the bearing roller, a sealing gasket is fixedly arranged on the inner side of the sealing cover, one end of the sealing cover is threaded through a positioning rod, a positioning groove is formed in the side wall of the storage tank on one side of the storage tank, and the positioning groove is matched with the positioning rod.
As a preferable technical scheme of the invention, the detection assembly comprises a mounting frame fixedly arranged on the outer side wall of the storage tank, one side of the mounting frame is provided with a through hole, the mounting frame is internally fixedly connected with a cooling fan, and two sides of the mounting frame are respectively fixedly connected with an oxygen detector and a carbon dioxide detector, so that the accurate detection of the components of gas in the storage environment in the storage tank can be realized, and the living environment of an internal sample is ensured.
As a preferable technical scheme of the invention, a valve groove is arranged in the storage box at one side of the storage groove, a heat dissipation channel is arranged at one side of the valve groove, an elastic piece is fixedly connected at one side of the valve groove, a piston block is fixedly connected at one side of the elastic piece, the piston block is in sliding connection with the valve groove, and the heat dissipation channel is communicated with the mounting frame.
As a preferable technical scheme of the invention, the storage boxes at two sides of the heat dissipation channel are respectively provided with a first through groove and a second through groove, the first through groove and the second through groove are respectively communicated with the oxygen detector and the carbon dioxide detector, and one sides of the first through groove and the second through groove are communicated with the storage groove.
As a preferable technical scheme of the invention, the side wall of the bearing roller is rotationally connected with the ball, and the ball is arranged in a sliding way with the inner wall of the storage groove.
The invention has the following advantages: when in use, a cell sample to be stored is placed in a test tube, then a sealing sleeve is fixedly sleeved at the port of the test tube, then the test tube is placed in a limiting groove, the test tube is positioned under the action of a rubber sleeve, then a rotary sealing cover drives a bearing roller to rotate, a roller drives a valve block to move downwards under the limiting action of a storage groove, the valve block moves downwards to separate an air guide groove from a communicating groove, the test tube is completely sealed and stored, in the long-distance transportation process, an operator can rotate the bearing roller at any time, the roller is placed in a protruding groove, the test tube is re-opened, gas components in the test tube are respectively fed into an oxygen detector and a carbon dioxide detector through a first through groove and a second through groove to detect the corresponding gas component content, and when the gas component content is lower than a standard value, the operator can open the oxygen tank and the carbon dioxide storage tube, the corresponding gas components are fed into the storage tank through the gas guide pipe and the transfer pipe under the action of the hot air blower, the test tube is heated, a standard cultivation environment is provided for cell organisms in the test tube, the activity of the cell organisms is guaranteed, in the process, the gas components are detected through the oxygen detector and the carbon dioxide detector, the electronic valve can be closed after the gas components reach the standard value, meanwhile, under the action of the piston block, when the temperature in the storage tank is higher, the gas pressure can drive the piston block to move to one side, the hot gas enters the installation frame through the heat dissipation channel, under the action of the heat dissipation fan, the heat dissipation and the temperature reduction in the storage tank are realized, the temperature in the storage tank is further guaranteed to be in a proper standard state, the activity of the cell organisms is guaranteed, and after the standard value is reached, the sealing cover can be rotated to drive the bearing roller to rotate, sealing the test tube, preserving, can prevent to take out the sample of polluting when the test tube, generally speaking the device operation is convenient to use, can carry out environmental simulation and monitoring to the cell sample alone, for the more energy saving of this kind of mode of low temperature storage, the simulation storage environment that simultaneously can be better can keep cell activity for a long time, and the practicality is higher.
Drawings
Fig. 1 is a schematic structural view of an ecological box body for cell-based biological sample storage.
Fig. 2 is an enlarged schematic view of the structure of fig. 1 a.
Fig. 3 is an enlarged schematic view of the structure B in fig. 1.
Fig. 4 is a schematic structural view of a carrying roller in an ecological box for cell-based biological sample storage.
Fig. 5 is a schematic diagram of the front surface of a storage box in an ecological box for cell-based biological sample storage.
In the figure: 1. a bottom plate; 2. a storage box; 3. a mounting groove; 4. a storage tank; 5. an oxygen tank; 6. an air heater; 7. a carbon dioxide storage tank; 8. a carrying roller; 9. an air duct; 10. a transfer tube; 11. a test tube; 12. a first through groove; 13. an electronic valve; 14. a protruding groove; 15. a mounting frame; 16. an oxygen detector; 17. a top plate; 18. a second through slot; 19. a carbon dioxide detector; 20. a heat dissipation fan; 21. a through port; 22. a heat dissipation channel; 23. a valve slot; 24. a piston block; 25. an elastic member; 26. a roller; 27. a valve block; 28. an air guide groove; 29. sealing sleeve; 30. a communication groove; 31. a reset member; 32. a limit groove; 33. a rubber sleeve; 34. a ball; 35. a sealing gasket; 36. sealing cover; 37. a positioning rod; 38. a positioning groove; 39. a storage mechanism; 40. an air guide assembly; 41. a seal assembly; 42. and a detection assembly.
Detailed Description
The preferred embodiments of the present invention will be described below with reference to the accompanying drawings, it being understood that the preferred embodiments described herein are for illustration and explanation of the present invention only, and are not intended to limit the present invention.
Example 1
Referring to fig. 1 to 5, an ecological box for cell-based biological sample storage, comprising a bottom plate 1, further comprising: a storage tank 2 fixedly arranged on one side of the bottom plate 1, a storage groove 4 is formed in the storage tank 2, and one side of the storage tank 2 is fixedly connected with a top plate 17; the storage mechanism 39 arranged in the storage tank 4 comprises a bearing roller 8, the bearing roller 8 and the storage tank 4 are movably arranged in a matching manner, a limit groove 32 is formed in one side of the bearing roller 8, a rubber sleeve 33 is fixedly connected to one side of the limit groove 32, a test tube 11 is movably arranged in the limit groove 32 on one side of the rubber sleeve 33, one end of the test tube 11 is sleeved with a sealing sleeve 29, two ends of the sealing sleeve 29 are provided with a communication groove 30, one side of the sealing sleeve 29 between the communication grooves 30 is fixedly connected with a reset piece 31, one side of the reset piece 31 is fixedly connected with a valve block 27, one end of the valve block 27 is provided with an air guide groove 28, the air guide groove 28 is of an I-shaped structure, the valve block 27 is in sliding connection with the sealing sleeve 29, one end of the valve block 27 is rotationally connected with a roller 26, a raised groove 14 is formed in the storage tank 2 on one side of the storage tank 4, an air guide component 40 is arranged between the raised groove 14, one end of the bearing roller 8 is provided with a sealing component 41, one end of the storage tank 2 is provided with a mounting groove 3, a hot air heater 6 is fixedly connected with a bottom plate 1, two sides of the hot air heater 6 are fixedly connected with the bottom plate 1, two sides of the bottom plate 1 are respectively provided with a carbon dioxide storage tank 7 and an oxygen tank 5, and an oxygen storage tank 6 are in sliding connection with the hot air guide component 6, and the hot air guide component is in the air tank 6, and the air guide component is in the air tank is in the air storage tank 6 through the automatic environment is in the air storage mode, and the air storage tank is in the air storage tank 2 is in the air storage tank 1.
When the device is actually used, a cell sample to be stored is placed in the test tube 11, then the sealing sleeve 29 is fixedly sleeved at the port of the test tube 11, then the test tube 11 is placed in the limiting groove 32, the test tube 11 is positioned under the action of the rubber sleeve 33, then the bearing roller 8 is rotated, the roller 26 drives the valve block 27 to move downwards under the limiting action of the storage groove 4 along with rotation, and the valve block 27 moves downwards to separate the air guide groove 28 from the communication groove 30, so that the test tube 11 is completely sealed and stored.
Example 2
Referring to fig. 1 to 5, the other contents of this embodiment are the same as those of embodiment 1, except that: the air guide assembly 40 comprises an air guide pipe 9 fixedly arranged in the storage box 2, two sides of the air guide pipe 9 are fixedly connected with a transfer pipe 10, one end of the transfer pipe 10 is fixedly connected with an electronic valve 13, and an air outlet end of the electronic valve 13 is communicated with the protruding groove 14.
The sealing assembly 41 comprises a sealing cover 36 fixedly arranged on one side of the bearing roller 8, a sealing gasket 35 is fixedly arranged on the inner side of the sealing cover 36, one end of the sealing cover 36 is threaded through a positioning rod 37, a positioning groove 38 is formed in the side wall of the storage tank 2 on one side of the storage tank 4, the positioning groove 38 is matched with the positioning rod 37, and positioning of the bearing roller 8 can be achieved through matching of the positioning rod 37 and the positioning groove 38 during actual storage.
The detection assembly 42 comprises a mounting frame 15 fixedly arranged on the outer side wall of the storage box 2, a through hole 21 is formed in one side of the mounting frame 15, a heat dissipation fan 20 is fixedly connected in the mounting frame 15, and an oxygen detector 16 and a carbon dioxide detector 19 are respectively and fixedly connected to two sides of the mounting frame 15.
A valve groove 23 is formed in the storage box 2 at one side of the storage groove 4, a heat dissipation channel 22 is formed at one side of the valve groove 23, an elastic piece 25 is fixedly connected to one side of the valve groove 23, a piston block 24 is fixedly connected to one side of the elastic piece 25, the piston block 24 is slidably connected with the valve groove 23, the heat dissipation channel 22 is communicated with the mounting frame 15, and a spring or an elastic metal sheet with telescopic properties can be used for the elastic piece 25.
The storage boxes 2 on two sides of the heat dissipation channel 22 are respectively provided with a first through groove 12 and a second through groove 18, the first through groove 12 and the second through groove 18 are respectively communicated with the oxygen detector 16 and the carbon dioxide detector 19, one sides of the first through groove 12 and the second through groove 18 are communicated with the storage groove 4, the side wall of the bearing roller 8 is rotationally connected with a plurality of balls 34, and the balls 34 are slidably arranged on the inner wall of the storage groove 4.
In the long-distance transportation process, an operator can rotate the bearing roller 8 at any time, the roller 26 is arranged in the protruding groove 14, the test tube 11 is opened again, the gas components in the test tube 11 are respectively fed into the oxygen detector 16 and the carbon dioxide detector 19 through the first through groove 12 and the second through groove 18 to detect the corresponding gas component content, when the gas component content is lower than a standard value, the operator can open the oxygen tank 5 and the carbon dioxide storage tube, the corresponding gas component is fed into the storage groove 4 through the air duct 9 and the transfer tube 10 under the action of the air heater 6, and the test tube 11 is heated, so that a standard cultivation environment is provided for cell organisms in the test tube 11, and the activity of the cell organisms is ensured.
In this process, through oxygen detector 16 and carbon dioxide detector 19 detect gaseous composition, can close electronic valve 13 after it reaches the standard value, simultaneously under the effect of piston piece 24, when the temperature in holding tank 4 is higher, atmospheric pressure can drive piston piece 24 to one side removal, in the steam gets into installing frame 15 through heat dissipation passageway 22, under the effect of heat dissipation fan 20, realize the heat dissipation cooling to holding tank 4, and then guarantee that the temperature in the holding tank 4 is in suitable standard state, guarantee the activity of cell biology, after reaching the standard value, can rotate sealed lid 36 and drive carrier roller 8 and rotate, seal test tube 11, save, pollution sample when can preventing to take out test tube 11.
Overall, the device operation is convenient, can carry out environmental simulation and monitoring to the cell sample alone, for the more energy saving of this kind of mode of low temperature storage, the simulation storage environment that simultaneously can be better, can long-time keep cell activity, the practicality is higher.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. An ecological box for storing based on a cell biological sample, which comprises a bottom plate, and is characterized by further comprising:
the storage box is arranged at one side of the bottom plate, a storage groove is formed in the storage box, and one side of the storage box is fixedly connected with the top plate;
the storage mechanism arranged in the storage tank comprises a bearing roller, wherein one side of the bearing roller is provided with a limit groove, one side of the limit groove is fixedly connected with a rubber sleeve, one side of the rubber sleeve is provided with a test tube, one end of the test tube is sleeved with a sealing sleeve, two ends of the sealing sleeve are provided with communication grooves, one side of the sealing sleeve between the communication grooves is fixedly connected with a reset piece, one side of the reset piece is fixedly connected with a valve block, one end of the valve block is provided with an air guide groove, the air guide groove is of an I-shaped structure, the valve block is in sliding connection with the sealing sleeve, one end of the valve block is rotationally connected with a roller, the storage tank on one side of the storage tank is internally provided with a bulge groove, an air guide assembly is arranged between the bulge grooves, one end of the bearing roller is provided with a sealing assembly, one end of the storage tank is provided with a mounting groove, a hot air heater is arranged in the mounting groove, two sides of the hot air heater are respectively provided with a carbon dioxide storage tank and an oxygen tank, the hot air heater is communicated with the air guide assembly, the side wall of the storage tank is provided with a detection assembly, and under a storage environment is rotationally driven by rotating the bearing roller, so that the environment simulation and the rapid conversion of a sealed storage mode can be automatically realized, and the environment simulation test can be realized, and the environment is independent environment simulation test can be realized, the environment is not influenced by the mutual influence of samples in the storage tank;
the detection assembly comprises a mounting frame fixedly arranged on the outer side wall of the storage box, one side of the mounting frame is provided with a through hole, the mounting frame is internally fixedly connected with a heat dissipation fan, and two sides of the mounting frame are respectively and fixedly connected with an oxygen detector and a carbon dioxide detector;
a valve groove is formed in the storage box at one side of the storage groove, a heat dissipation channel is formed in one side of the valve groove, an elastic piece is fixedly connected to one side of the valve groove, a piston block is fixedly connected to one side of the elastic piece, and the heat dissipation channel is communicated with the mounting frame;
the storage boxes on two sides of the heat dissipation channel are respectively provided with a first through groove and a second through groove, and the first through groove and the second through groove are respectively communicated with the oxygen detector and the carbon dioxide detector.
2. The ecological box for cell-based biological sample storage according to claim 1, wherein the air guide assembly comprises an air guide pipe fixedly arranged in the storage box, two sides of the air guide pipe are fixedly connected with a transfer pipe, one end of the transfer pipe is fixedly connected with an electronic valve, and an air outlet end of the electronic valve is communicated with the convex groove.
3. The ecological box for cell-based biological sample storage according to claim 1, wherein the sealing assembly comprises a sealing cover arranged on one side of the bearing roller, a sealing gasket is arranged on the inner side of the sealing cover, one end of the sealing cover is threaded through a positioning rod, a positioning groove is formed in the side wall of the storage box on one side of the storage groove, and the positioning groove is matched with the positioning rod.
4. The ecological box for storing cell-based biological samples according to claim 1, wherein the side wall of the bearing roller is rotatably connected with a ball, and the ball is slidably arranged with the inner wall of the storage tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211015681.XA CN115251047B (en) | 2022-08-24 | 2022-08-24 | Ecological box for storing cell-based biological samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211015681.XA CN115251047B (en) | 2022-08-24 | 2022-08-24 | Ecological box for storing cell-based biological samples |
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| Publication Number | Publication Date |
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| CN115251047A CN115251047A (en) | 2022-11-01 |
| CN115251047B true CN115251047B (en) | 2023-08-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202211015681.XA Active CN115251047B (en) | 2022-08-24 | 2022-08-24 | Ecological box for storing cell-based biological samples |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115626386B (en) * | 2022-12-07 | 2023-03-17 | 山东锐华氟业有限公司 | Sulfur tetrafluoride transportation storage tank |
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| CN108855261A (en) * | 2018-06-22 | 2018-11-23 | 无锡南理工科技发展有限公司 | A kind of environmental-protecting chemical reagent storing apparatus of chemical engineering |
| CN109552878A (en) * | 2018-10-18 | 2019-04-02 | 浙江泰林生物技术股份有限公司 | A kind of device that article is quickly spread out of suitable for closed clean environment |
| CN212560286U (en) * | 2020-05-27 | 2021-02-19 | 苏州市绿微康生物科技有限公司 | Feed supplement device of enzyme preparation fermentation cylinder |
| CN112772631A (en) * | 2020-12-30 | 2021-05-11 | 武汉北度生物科技有限公司 | Based on ecological case is used in biological sample storage of cell |
| CN114476383A (en) * | 2022-02-28 | 2022-05-13 | 诺依曼(山东)物联网研究院有限公司 | Storage device is used in smart machine research and development |
-
2022
- 2022-08-24 CN CN202211015681.XA patent/CN115251047B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108855261A (en) * | 2018-06-22 | 2018-11-23 | 无锡南理工科技发展有限公司 | A kind of environmental-protecting chemical reagent storing apparatus of chemical engineering |
| CN109552878A (en) * | 2018-10-18 | 2019-04-02 | 浙江泰林生物技术股份有限公司 | A kind of device that article is quickly spread out of suitable for closed clean environment |
| CN212560286U (en) * | 2020-05-27 | 2021-02-19 | 苏州市绿微康生物科技有限公司 | Feed supplement device of enzyme preparation fermentation cylinder |
| CN112772631A (en) * | 2020-12-30 | 2021-05-11 | 武汉北度生物科技有限公司 | Based on ecological case is used in biological sample storage of cell |
| CN114476383A (en) * | 2022-02-28 | 2022-05-13 | 诺依曼(山东)物联网研究院有限公司 | Storage device is used in smart machine research and development |
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