CN112880297A - Microorganism preservation device - Google Patents
Microorganism preservation device Download PDFInfo
- Publication number
- CN112880297A CN112880297A CN202110048094.XA CN202110048094A CN112880297A CN 112880297 A CN112880297 A CN 112880297A CN 202110048094 A CN202110048094 A CN 202110048094A CN 112880297 A CN112880297 A CN 112880297A
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- Prior art keywords
- preservation
- pipe
- storage box
- test tube
- box
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- 238000004321 preservation Methods 0.000 title claims abstract description 48
- 244000005700 microbiome Species 0.000 title claims abstract description 18
- 230000007246 mechanism Effects 0.000 claims abstract description 4
- 238000009434 installation Methods 0.000 claims abstract 4
- 230000017105 transposition Effects 0.000 claims description 7
- 239000007789 gas Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000012188 paraffin wax Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 229940057995 liquid paraffin Drugs 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 238000004378 air conditioning Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Images
Classifications
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D31/00—Other cooling or freezing apparatus
- F25D31/005—Combined cooling and heating devices
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Combustion & Propulsion (AREA)
- Physics & Mathematics (AREA)
- Mechanical Engineering (AREA)
- Thermal Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a microorganism preservation device, which comprises a preservation box, wherein a plurality of support plates are sequentially arranged on two side walls in the preservation box from top to bottom, a plurality of strip-shaped sliding groove bodies are fixedly arranged on the upper surfaces of the support plates, strip plates are slidably connected inside the sliding groove bodies, a plurality of circular grooves for placing test tubes are arranged on the upper surfaces of the strip plates, clamping mechanisms for fixing the test tubes are arranged on the upper surfaces of the strip plates and close to each test tube groove, an installation opening is arranged in the middle of the upper surface of the preservation box, a seat body is fixedly arranged on the inner side wall of the installation opening, a through hole is arranged between the upper surface and the lower surface of the seat body, one end of a main vent pipe is rotatably connected to the lower side inside of the through hole through a bearing, one end of a rotating shaft is fixedly arranged at the other end of the main vent pipe, and the preservation, so that more test tubes containing the strains can be placed when the test tubes are stored upright.
Description
Technical Field
The invention relates to the technical field of preservation devices, in particular to a microorganism preservation device.
Background
The microbial strain is an important biological resource, the strain preservation is important basic work of the microorganism, the strain preservation is to utilize all conditions to make the strain not dead, not weak and not changed so as to be convenient for research and application, the strain preservation methods are various, but the principles are largely the same, and an environment suitable for long-term dormancy is not created for excellent strains, namely, the strains are dry, low-temperature, lack of oxygen and nutrients and the like, so that the metabolic activity of the microorganism is in the lowest state, but the microorganism cannot die, and the preservation purpose is achieved. According to different strains or different requirements, different preservation methods are required to be selected, and in general, the freezing preservation, the inclined plane preservation and the liquid paraffin preservation are commonly used, wherein the liquid paraffin is preserved in a way that sterile paraffin is added on the inclined plane on which the strains grow, the use amount of the sterile paraffin is 1cm higher than the top end of the inclined plane, so that the strains are isolated from the air, then the test tubes are erected and placed in a box body at 4-15 ℃ for preservation, but the existing preservation box has certain defects, and the occupied position is larger because the test tubes are required to be erected during preservation, so that the preservation box cannot preserve more test tubes filled with the strains.
Disclosure of Invention
The invention aims to provide a microorganism preservation device, which solves the problem that the preservation mode of liquid paraffin proposed in the background technology is to add sterile paraffin on an inclined plane on which bacteria grow well, wherein the use amount of the sterile paraffin is 1cm higher than the top end of the inclined plane, so that the bacteria are isolated from air, and then a test tube is erected and placed in a box body at 4-15 ℃ for preservation, but the existing preservation box has certain defects, and the test tube needs to be erected when being preserved, so that the occupied position is large, and the preservation box cannot preserve more test tubes filled with the bacteria.
In order to achieve the purpose, the invention provides the following technical scheme: a preservation device for microorganisms comprises a preservation box, wherein a plurality of support plates are sequentially arranged on two side walls of the interior of the preservation box from top to bottom, a plurality of strip-shaped sliding groove bodies are fixedly arranged on the upper surfaces of the support plates, a strip plate is slidably connected inside the sliding groove bodies, a plurality of circular grooves for holding test tubes are arranged on the upper surface of the strip plate, clamping mechanisms for fixing the test tubes are arranged on the upper surfaces of the strip plate and close to each test tube groove, a mounting hole is formed in the middle of the upper surface of the preservation box, a seat body is fixedly arranged on the inner side wall of the mounting hole, a through hole is formed between the upper surface and the lower surface of the seat body, one end of a main vent pipe is rotatably connected to the lower side of the interior of the through hole through a bearing, one end of a rotating shaft is fixedly arranged at the other end of the, the output of transposition motor run through the lower surface of hutch and with the other end fixed connection of pivot, main breather pipe sets up between the backup pad of both sides, the inside upside of through-hole rotates the one end that is connected with vice breather pipe through the bearing, the other end symmetric connection of vice breather pipe has the one end of connecting pipe, the upper surface of hutch and the both sides that are located vice breather pipe are equipped with refrigerator and heating ware respectively, the output of refrigerator and the output of heating ware are respectively in the other end fixed connection of two connecting pipes, the lateral wall of main breather pipe and the upper portion that is located every backup pad all are equipped with the gas pocket, the gas pocket is connected with the one end of scattered gas pipe spare, the lateral wall lower part of hutch is equipped with the temperature sensor who detects its inside temperature, the case mouth of hu.
Preferably, fixture includes two bracing pieces, two the one end difference fixed mounting of bracing piece just is located the both sides position of test tube circular slot at the upper surface of slat, the other end fixed mounting of bracing piece has the nut piece, the inside threaded connection of nut piece has the screw rod, the one end rotation that the screw rod is close to the test tube circular slot is connected with the semicircle ring body, and the test tube centre gripping is between two semicircle ring bodies.
Preferably, the air dispersing pipe fitting comprises an air dispersing pipe, a plurality of air dispersing holes are formed in the lower side wall of the air dispersing pipe, and air dispersing nozzles are installed on the air dispersing holes.
Preferably, the inside both sides wall symmetry of spout body is equipped with the guide way, the both sides wall symmetry of slat is equipped with the gib block, two the gib block is sliding connection respectively in two the guide way.
Preferably, the connecting pipe is provided with an electromagnetic valve.
Preferably, one end of the screw rod, which is far away from the circular groove of the test tube, is fixedly provided with a rotating handle.
Preferably, a support frame body is arranged on the lower surface of the storage box.
Compared with the prior art, the invention has the beneficial effects that: a preservation device for microorganisms is characterized in that two nut blocks and two screws are arranged in a matched mode, when two rotating handles are screwed, two half circular ring bodies can be driven to clamp test tubes in test tube grooves, the test tubes are fixed on battens, and the preservation box is prevented from shaking in the transportation process;
the strip plate can slide into the sliding groove body through the matching arrangement of the guide groove and the guide strip, so that a plurality of test tubes can be arranged on the internal support plate of the storage box to be convenient to take from the storage box;
through the cooperation setting of refrigerator and heater, at the transmission by main breather pipe, vice breather pipe and connecting pipe, can be with steam or air conditioning conveying go into in the gas pipe that looses, thereby in scattering into the case of preserving by a plurality of gas nozzle that looses, can improve or reduce the temperature in the case of preserving, make the temperature in the case of preserving keep 4-15 degrees centigrade always, through the cooperation setting of transposition motor and pivot, rotatable main breather pipe, thereby can improve the diffusion rate of air conditioning or steam, guarantee that the temperature in the case of preserving improves or reduces in the short time, improve the bacterial survival rate in the examination pipe.
Drawings
FIG. 1 is a schematic sectional front view of the present invention;
FIG. 2 is an enlarged schematic view of the structure at A in FIG. 1;
FIG. 3 is a schematic top cross-sectional view of the present invention;
FIG. 4 is an enlarged view of the structure at B in FIG. 3;
fig. 5 is a schematic front view of the present invention.
In the figure: 1-storage box, 2-support plate, 3-chute body, 4-strip plate, 5-test tube groove, 6-seat body, 7-main vent pipe, 8-rotating shaft, 9-indexing motor, 10-auxiliary vent pipe, 11-connecting pipe, 12-refrigerator, 13-heater, 14-temperature sensor, 15-box door, 16-support rod, 17-nut block, 18-screw, 19-semi-circular ring body, 20-air dispersing pipe, 21-air dispersing nozzle, 22-guide groove, 23-guide bar, 24-electromagnetic valve, 25-rotating handle and 26-support frame body.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: a microorganism preservation device comprises a preservation box 1, wherein a plurality of support plates 2 are sequentially arranged on two side walls in the interior of the preservation box 1 from top to bottom, a plurality of strip-shaped sliding groove bodies 3 are fixedly arranged on the upper surfaces of the support plates 2, a strip plate 4 is slidably connected in the sliding groove bodies 3, a plurality of circular grooves 5 for placing test tubes are arranged on the upper surface of the strip plate 4, clamping mechanisms for fixing the test tubes are arranged on the upper surfaces of the strip plate 4 and close to each test tube groove 5, a mounting hole is formed in the middle position of the upper surface of the preservation box 1, a seat body 6 is fixedly arranged on the inner side wall of the mounting hole, a through hole is formed between the upper surface and the lower surface of the seat body 6, one end of a main vent pipe 7 is rotatably connected to the lower side of the interior of the through hole through a bearing, one end of a rotating shaft 8 is fixedly arranged at the other end of the main vent pipe 7, the output end of the transposition motor 9 penetrates through the lower surface of the storage box 1 and is fixedly connected with the other end of the rotating shaft 8, the main vent pipe 7 can be rotated through the matching arrangement of the transposition motor 9 and the rotating shaft 8, so that the diffusion rate of cold air or hot air can be improved, the temperature in the storage box 1 can be improved or reduced in a short time, the survival rate of strains in a test tube can be improved, the main vent pipe 7 is arranged between the support plates 2 at two sides, the upper side in the through hole is rotatably connected with one end of the auxiliary vent pipe 10 through a bearing, the other end of the auxiliary vent pipe 10 is symmetrically connected with one end of the connecting pipe 11, the refrigerator 12 and the heater 13 are respectively arranged on the upper surface of the storage box 1 and on two sides of the auxiliary vent pipe 10, the output end of the refrigerator 12 and the output end of the heater 13 are respectively fixedly connected with the other ends of the two connecting pipes 11, the air hole is connected with one end of an air dispersing pipe fitting, the lower part of the side wall of the storage box 1 is provided with a temperature sensor 14 for detecting the internal temperature of the storage box, the temperature in the storage box 1 can be monitored in real time through the temperature sensor 14, and the box opening of the storage box 1 is covered with a box door 15.
Particularly, fixture includes two bracing pieces 16, two the one end difference fixed mounting of bracing piece 16 is at the upper surface of slat 4 and is located the both sides position of test tube circular slot 5, the other end fixed mounting of bracing piece 16 has nut piece 17, the inside threaded connection of nut piece 17 has screw rod 18, screw rod 18 is close to the one end rotation of test tube circular slot 5 and is connected with semi-torus 19, and the test tube centre gripping sets up through the cooperation of two nut pieces 17 and two screw rods 18 between two semi-torus 19, when twisting two turning handles 25, can drive two semi-torus 19 and carry out the clamping effect to the test tube in the test tube groove 5 to fix the test tube on slat 4, prevent that hutch 1 from rocking in the transportation.
Specifically, the air-dispersing pipe member includes an air-dispersing pipe 20, a plurality of air-dispersing holes are formed in the lower side wall of the air-dispersing pipe 20, the air-dispersing holes are provided with air-dispersing nozzles 21, and hot air or cold air can be delivered into the air-dispersing pipe 20 by the transmission of the main vent pipe 7, the auxiliary vent pipe 10 and the connecting pipe 11 through the matching arrangement of the refrigerator 12 and the heater 13, so that the temperature in the storage box 1 can be increased or decreased by dispersing the plurality of air-dispersing nozzles 21 into the storage box 1, and the temperature in the storage box 1 can be kept at 4-15 ℃.
Particularly, the inside both sides wall symmetry of spout body 3 is equipped with guide way 22, the both sides wall symmetry of slat 4 is equipped with gib block 23, two gib block 23 sliding connection is two respectively in guide way 22, through the cooperation setting of guide way 22 and gib block 23, can slide into the spout body 3 with slat 4 in to can set up a plurality of test tube on the inside backup pad 2 of keeping case 1, conveniently take from keeping in case 1.
Specifically, the connection pipe 11 is provided with an electromagnetic valve 24 to prevent cold air or hot air from entering the heater 13 and the refrigerator 12, respectively.
Specifically, the screw 18 is fixedly provided with a rotating handle 25 at one end far away from the test tube circular groove 5, so that the screw 18 is convenient to understand.
Specifically, the storage box 1 is provided with a support frame 26 on the lower surface thereof to support the storage box 1.
The working principle is as follows: according to the invention, through the matching arrangement of the two nut blocks 17 and the two screw rods 18, when the two rotating handles 25 are screwed, the two half circular ring bodies 19 can be driven to clamp test tubes in the test tube grooves 5, so that the test tubes are fixed on the strip plates 4, the storage box 1 is prevented from shaking in the transportation process, through the matching arrangement of the guide grooves 22 and the guide strips 23, the strip plates 4 can be slid into the sliding groove bodies 3, so that a plurality of test tubes can be arranged on the inner supporting plates 2 of the storage box 1, the storage box 1 is convenient to take from, because the storage box 1 is sequentially provided with a plurality of supporting plates 2 from top to bottom, when the test tubes are stored vertically, more test tubes containing strains can be placed, through the matching arrangement of the refrigerator 12 and the heater 13, the hot air or cold air can be conveyed into the air dispersing pipe 20 through the main vent pipe 7, the auxiliary vent pipe 10 and the connecting pipe 11, so that a plurality of dispersing nozzles 21 can be dispersed into the storage box 1, the temperature in the preservation box 1 can be increased or decreased, so that the temperature in the preservation box 1 is always kept at 4-15 ℃, the main vent pipe 7 can be rotated through the matching arrangement of the transposition motor 9 and the rotating shaft 8, the diffusion rate of cold air or hot air can be increased, the temperature in the preservation box 1 can be increased or decreased in a short time, the survival rate of strains in a test tube can be increased, and the temperature in the preservation box 1 can be monitored in real time through the temperature sensor 14.
In the description of the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral part; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The standard parts used in the invention can be purchased from the market, the special-shaped parts can be customized according to the description of the specification and the accompanying drawings, the specific connection mode of each part adopts conventional means such as bolts, rivets, welding and the like mature in the prior art, the machines, the parts and equipment adopt conventional models in the prior art, and the circuit connection adopts the conventional connection mode in the prior art, so that the detailed description is omitted.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A preservation device for microorganisms, comprising a preservation box (1), characterized in that: the test tube storage box is characterized in that a plurality of support plates (2) are sequentially arranged on two inner side walls of the storage box (1) from top to bottom, a plurality of strip-shaped sliding groove bodies (3) are fixedly arranged on the upper surface of each support plate (2), a strip plate (4) is slidably connected to the inner portion of each sliding groove body (3), a plurality of circular grooves (5) for containing test tubes are formed in the upper surface of each strip plate (4), clamping mechanisms for fixing the test tubes are arranged on the upper surface of each strip plate (4) and in positions close to each test tube groove (5), an installation opening is formed in the middle of the upper surface of the storage box (1), a seat body (6) is fixedly arranged on the inner side wall of the installation opening, a through hole is formed between the upper surface and the lower surface of the seat body (6), one end of a main vent pipe (7) is rotatably connected to the lower side of the inner portion of the through hole through, the lower surface middle position fixed mounting of keeping case (1) has transposition motor (9), the output of transposition motor (9) run through the lower surface of keeping case (1) and with the other end fixed connection of pivot (8), main breather pipe (7) set up between the backup pad (2) of both sides, the inside upside of through-hole is rotated through the bearing and is connected with the one end of vice breather pipe (10), the other end symmetric connection of vice breather pipe (10) has the one end of connecting pipe (11), the upper surface of keeping case (1) and the both sides that are located vice breather pipe (10) are equipped with refrigerator (12) and heater (13) respectively, the output of refrigerator (12) and the output of heater (13) are respectively in the other end fixed connection of two connecting pipes (11), the lateral wall of main breather pipe (7) and the upper portion that is located every backup pad (2) all are equipped with the gas pocket, the air hole is connected with one end of an air dispersing pipe fitting, the lower part of the side wall of the storage box (1) is provided with a temperature sensor (14) for detecting the internal temperature of the storage box, and the box opening of the storage box (1) is covered with a box door (15).
2. A device for the preservation of microorganisms according to claim 1, characterized in that: fixture includes two bracing pieces (16), two the one end difference fixed mounting of bracing piece (16) just is located the both sides position of test tube circular slot (5) at the upper surface of slat (4), the other end fixed mounting of bracing piece (16) has nut piece (17), the inside threaded connection of nut piece (17) has screw rod (18), the one end rotation that screw rod (18) are close to test tube circular slot (5) is connected with semi-ring body (19), and the test tube centre gripping is between two semi-ring bodies (19).
3. A device for the preservation of microorganisms according to claim 1, characterized in that: the air dispersing pipe fitting comprises an air dispersing pipe (20), a plurality of air dispersing holes are formed in the lower side wall of the air dispersing pipe (20), and air dispersing nozzles (21) are installed on the air dispersing holes.
4. A device for the preservation of microorganisms according to claim 1, characterized in that: inside both sides wall symmetry of spout body (3) is equipped with guide way (22), the both sides wall symmetry of slat (4) is equipped with gib block (23), two gib block (23) sliding connection respectively is in two in guide way (22).
5. A device for the preservation of microorganisms according to claim 1, characterized in that: and an electromagnetic valve (24) is arranged on the connecting pipe (11).
6. A device for the preservation of microorganisms according to claim 2, characterized in that: and a rotating handle (25) is fixedly arranged at one end of the screw rod (18) far away from the test tube circular groove (5).
7. A device for the preservation of microorganisms according to claim 1, characterized in that: the lower surface of the storage box (1) is provided with a support frame body (26).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110048094.XA CN112880297A (en) | 2021-01-14 | 2021-01-14 | Microorganism preservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110048094.XA CN112880297A (en) | 2021-01-14 | 2021-01-14 | Microorganism preservation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112880297A true CN112880297A (en) | 2021-06-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110048094.XA Pending CN112880297A (en) | 2021-01-14 | 2021-01-14 | Microorganism preservation device |
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| Country | Link |
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| CN (1) | CN112880297A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114857822A (en) * | 2022-06-01 | 2022-08-05 | 深圳大学总医院 | Anesthetic is cooling box for operating room |
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| EP3336455A1 (en) * | 2016-12-16 | 2018-06-20 | KD Maennel GmbH | Cryogenic storage device for storing samples, in particular for low temperature storage of biological samples |
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Application publication date: 20210601 |