CN115227587B - 一种亲水性虾青素乳化分散油的制备方法及其应用 - Google Patents
一种亲水性虾青素乳化分散油的制备方法及其应用 Download PDFInfo
- Publication number
- CN115227587B CN115227587B CN202210773805.4A CN202210773805A CN115227587B CN 115227587 B CN115227587 B CN 115227587B CN 202210773805 A CN202210773805 A CN 202210773805A CN 115227587 B CN115227587 B CN 115227587B
- Authority
- CN
- China
- Prior art keywords
- astaxanthin
- hydrophilic
- oil
- tween
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims abstract description 139
- 239000001168 astaxanthin Substances 0.000 title claims abstract description 139
- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 139
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims abstract description 139
- 229940022405 astaxanthin Drugs 0.000 title claims abstract description 139
- 238000002360 preparation method Methods 0.000 title claims abstract description 36
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000006185 dispersion Substances 0.000 claims abstract description 46
- 239000000839 emulsion Substances 0.000 claims abstract description 39
- 239000002131 composite material Substances 0.000 claims abstract description 35
- 230000001804 emulsifying effect Effects 0.000 claims abstract description 33
- 238000003756 stirring Methods 0.000 claims abstract description 31
- 238000002156 mixing Methods 0.000 claims abstract description 30
- 238000010438 heat treatment Methods 0.000 claims abstract description 25
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 22
- 239000000203 mixture Substances 0.000 claims abstract description 20
- 239000003381 stabilizer Substances 0.000 claims abstract description 19
- 150000003904 phospholipids Chemical class 0.000 claims abstract description 18
- 229920000136 polysorbate Polymers 0.000 claims abstract description 16
- 238000001816 cooling Methods 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 3
- 238000000265 homogenisation Methods 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 11
- 235000013305 food Nutrition 0.000 claims description 6
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 claims description 5
- 239000002537 cosmetic Substances 0.000 claims description 4
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 3
- 229920001214 Polysorbate 60 Polymers 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 2
- 239000000787 lecithin Substances 0.000 claims description 2
- 235000010445 lecithin Nutrition 0.000 claims description 2
- 229940067606 lecithin Drugs 0.000 claims description 2
- 239000008347 soybean phospholipid Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 239000002245 particle Substances 0.000 description 15
- 239000002884 skin cream Substances 0.000 description 6
- 235000012055 fruits and vegetables Nutrition 0.000 description 5
- 241000168517 Haematococcus lacustris Species 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000007908 nanoemulsion Substances 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 235000020419 dragon fruit juice Nutrition 0.000 description 2
- -1 ketone carotenoid Chemical class 0.000 description 2
- 235000020124 milk-based beverage Nutrition 0.000 description 2
- 230000002000 scavenging effect Effects 0.000 description 2
- 238000013112 stability test Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- 235000015094 jam Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
- A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
- A23G9/36—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
- A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
- A23G9/42—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/035—Organic compounds containing oxygen as heteroatom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/066—Multiple emulsions, e.g. water-in-oil-in-water
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4993—Derivatives containing from 2 to 10 oxyalkylene groups
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Nutrition Science (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Emergency Medicine (AREA)
- Microbiology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Dispersion Chemistry (AREA)
- Dermatology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
- Medicinal Preparation (AREA)
Abstract
本发明公开了一种亲水性虾青素乳化分散油的制备方法及其应用,制备方法包括以下制备步骤:步骤一、将吐温和磷脂混合,加热至50~60℃,搅拌至混合均匀,得复合乳化剂;步骤二、将复合乳化剂和丙二醇混合,加热至50~60℃,搅拌至充分混合均匀,得复合乳化稳定剂;步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油混合,加热至50~60℃,搅拌至充分混合均匀,降至常温,高压均质,得亲水性虾青素乳化分散油。本发明具有亲水性强、稳定性高、应用广泛的特点。
Description
技术领域
本发明涉及一种虾青素乳化分散油,特别是一种亲水性虾青素乳化分散油的制备方法及其应用。
背景技术
从雨生红球藻中获得的天然虾青素,是一种橙红色酮式类胡萝卜素,天然虾青素能抑制食品的脂质氧化,有效地清除体内的自由基,具有提升机体的免疫力等多方面的生物活性。因此,从雨生红球藻中提取的虾青素,在食品、化妆护肤品等领域中有广泛的应用前景。
但是天然虾青素为脂溶性物质,在水溶性体系中溶解性极差,其应用形态仅局限于胶囊、粉剂。另外,由于虾青素为长链不饱和共轭双键结构体系,因此性质不稳定,易于异构化和降解。虾青素对氧气、太阳光和紫外线照射、温度均敏感,加热处理、碱性等条件都会引起虾青素的异构化,这些方面极大地限制了虾青素的广泛应用。而目前,为了增强虾青素的稳定性,增加虾青素的储存时间,一般是把虾青素制得乳液。但是乳液中油相采用的是虾青素含量比较低的虾青素油,通常仅为5%以下,而对于虾青素含量在5%以上的高浓度的虾青素油(目前市面上提取到的虾青素油中,高含量的虾青素油为10%),仍然无法很好地控制其稳定性,而且在制备成乳液过程中,会添加其他的油相、乙醇等助剂,影响成品的纯度,使其应用领域受限,并无法制得具有高浓度的亲水性虾青素乳化分散油,应用在各个领域。
发明内容
本发明的目的在于,提供一种亲水性虾青素乳化分散油的制备方法及其应用。本发明具有亲水性强、稳定性高、应用广泛的特点。
本发明的技术方案:一种亲水性虾青素乳化分散油的制备方法,包括以下制备步骤:
步骤一、将吐温和磷脂混合,加热至50~60℃,搅拌至混合均匀,得复合乳化剂;
步骤二、将复合乳化剂和丙二醇混合,加热至50~60℃,搅拌至充分混合均匀,得复合乳化稳定剂;
步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油混合,加热至50~60℃,搅拌至充分混合均匀,降至常温,通过高压均质机超细化均质至纳米级,得亲水性虾青素乳化分散油。
前述的亲水性虾青素乳化分散油的制备方法中,步骤一的搅拌时间为5~10min,步骤二的搅拌时间为2~5min,步骤三的搅拌时间为1~2min,高压超细化均质一次。
前述的亲水性虾青素乳化分散油的制备方法中,所述步骤一中,吐温和磷脂的质量比为3:1~6:1。
前述的亲水性虾青素乳化分散油的制备方法中,所述步骤二中,复合乳化剂和丙二醇的质量比为5:1~9:1。
前述的亲水性虾青素乳化分散油的制备方法中,所述步骤三中,复合乳化稳定剂与虾青素油的质量比为3:1~9:1。
前述的亲水性虾青素乳化分散油的制备方法中,所述吐温采用吐温系列的乳化剂,所述磷脂采用磷脂酰胆碱或胆碱磷脂的含量在25~50%的磷脂,所述丙二醇的纯度在99%以上。
前述的亲水性虾青素乳化分散油的制备方法中,所述吐温采用吐温80、吐温60、吐温40或者吐温20;所述磷脂采用大豆磷脂或者卵磷脂。
前述的亲水性虾青素乳化分散油的制备方法中,所述步骤三中的高压均质的压力为15/65MPpa~30/150MPa。
还包括上述的亲水性虾青素乳化分散油的应用,所述亲水性虾青素乳化分散油在制备化妆品上的应用,所述化妆品包括乳液。
还包括亲水性虾青素乳化分散油的应用,所述亲水性虾青素乳化分散油在制备食品上的应用。
与现有技术相比,本发明的有益效果为:
本发明采用具有亲水和亲油两性的吐温和磷脂所组成的复合乳化剂,吐温是亲水性大于亲油性,磷脂是亲水性小于亲油性,经过一定配比,得到的复合乳化剂的HLB(亲水亲油平衡值)达到13.5-15的稳定体系,一侧为亲水基团,另一侧为亲油基团;其中的亲油基团作用于虾青素油,亲水基团作用于丙二醇,在两相表面排列,在虾青素油微粒表面形成稳定的保护界面活性,对虾青素油的稳定性和虾青素油的降解形成了很好的保护。
加入的丙二醇,能够提高复合乳化稳定剂的流动性,降低油的粘稠度低,减少沉淀物。
制成的亲水性虾青素乳化分散油,不仅含有的虾青素含量高,而且能够保证高浓度的虾青素油在室温下,存放二年不降解,不发生沉淀、分层、变色情况,对比原料的虾青素油和低浓度的虾青素油的稳定性有很大的增强和提高,还具有高亲水性,作为功能性配料,可根据需要与亲水性基质材料任意混合,溶入其中,拓宽了虾青素的应用领域和范围,如在食品,包括饮料、冰淇淋、果酱等,化妆品、护肤品中等领域中使用,充分发挥虾青素的抗氧化、清除自由基的功能。
整个制备工艺简单、易行、生物环保,每个步骤所需时间少,可连续生产,工作效率高。
附图说明
图1是实施例2的亲水性虾青素乳化分散油的稳定性图;
图2是实施例1的亲水性虾青素乳化分散油的粒径图;
图3是实施例2的亲水性虾青素乳化分散油的粒径图;
图4是实施例3的亲水性虾青素乳化分散油的粒径图;
图5是实施例4的亲水性虾青素乳化分散油的粒径图;
图6是实施例5的虾青素乳液的粒径图。
具体实施方式
下面结合实施例对本发明作进一步的说明,但并不作为对本发明限制的依据。
一种亲水性虾青素乳化分散油的制备方法,包括以下制备步骤:
步骤一、将吐温和磷脂按照质量比为3:1~6:1混合,水浴加热至50~60℃,磁力搅拌5~10min至充分混合均匀,得复合乳化剂;所述吐温采用吐温系列的乳化剂,所述磷脂采用磷脂酰胆碱或胆碱磷脂的含量在25~50%的磷脂;
步骤二、将复合乳化剂和丙二醇按照质量比为5:1~9:1混合,水浴加热至50~60℃,磁力搅拌2~5min至充分混合均匀,得复合乳化稳定剂;所述丙二醇的纯度在99%以上;
步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油按照质量比为3:1~9:1混合,水浴加热至40~60℃,磁力搅拌1~2min至充分混合均匀,降至常温,高压超细化均质一次至颗粒为400nm以下,均质的压力为15/65MPpa~30/150MPa,得亲水性虾青素乳化分散油。所述虾青素油从雨生红球藻中提取得到。
实施例1:
一种亲水性虾青素乳化分散油的制备方法,包括以下制备步骤:
步骤一、将吐温60和磷脂Pc25按照质量比为5:1混合,水浴加热至50℃,磁力搅拌8min至充分溶解、混合均匀,得复合乳化剂;
步骤二、将复合乳化剂和丙二醇按照质量比为6:1混合,水浴加热至50℃,磁力搅拌3min至充分混合均匀,得复合乳化稳定剂;所述丙二醇的纯度在99%以上;
步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油按照质量比为7.3:1混合,水浴加热至45℃,磁力搅拌1min至充分混合均匀,降至常温,高压超细化均质一次,高压均质的压力为15/75MPa,得亲水性虾青素乳化分散油。
亲水性虾青素乳化分散油中含有的虾青素油的含量为12%。
实施例2:
一种亲水性虾青素乳化分散油的制备方法,包括以下制备步骤:
步骤一、将吐温80和磷脂Pc35按照质量比为3.6:1混合,水浴加热至50℃,磁力搅拌8min至充分溶解、混合均匀,得复合乳化剂;
步骤二、将复合乳化剂和丙二醇按照质量比为5.3:1混合,水浴加热至50℃,磁力搅拌3min至充分混合均匀,得复合乳化稳定剂;所述丙二醇的纯度在99%以上;
步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油按照质量比为5.25:1混合,水浴加热至45℃,磁力搅拌1min至充分混合均匀,降至常温,高压超细化均质一次,高压均质的压力为20/100MPa,得亲水性虾青素乳化分散油。
亲水性虾青素乳化分散油中的含有的虾青素油的含量为16%。
实施例3:
一种亲水性虾青素乳化分散油的制备方法,包括以下制备步骤:
步骤一、将吐温40和磷脂Pc25按照质量比为4.7:1混合,水浴加热至50℃,磁力搅拌8min至充分溶解、混合均匀,得复合乳化剂;
步骤二、将复合乳化剂和丙二醇按照质量比为6.8:1混合,水浴加热至50℃,磁力搅拌3min至充分混合均匀,得复合乳化稳定剂;所述丙二醇的纯度在99%以上;
步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油按照质量比为3.5:1混合,水浴加热至45℃,磁力搅拌1min至充分混合均匀,降至常温,高压超细化均质一次,高压均质的压力为25/120MPa,得亲水性虾青素乳化分散油。
亲水性虾青素乳化分散油中含有的虾青素油的含量为22%。
实施例4:
一种亲水性虾青素乳化分散油的制备方法,包括以下制备步骤:
步骤一、将吐温20和磷脂Pc45按照质量比为5.7:1混合,水浴加热至50℃,磁力搅拌8min至充分溶解、混合均匀,得复合乳化剂;
步骤二、将复合乳化剂和丙二醇按照质量比为8.4:1混合,水浴加热至50℃,磁力搅拌3min至充分混合均匀,得复合乳化稳定剂;所述丙二醇的纯度在99%以上;
步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油按照质量比为3:1混合,水浴加热至45℃,磁力搅拌1min至充分混合均匀,降至常温,高压超细化均质一次,高压均质的压力为25/145MPa,得亲水性虾青素乳化分散油。
亲水性虾青素乳化分散油中含有的虾青素油的含量为25%。
实施例5:
本方案还包括虾青素油在护肤品中的应用。
一种虾青素乳液的制备方法:
将实施例2制得的亲水性虾青素乳化分散油与去离子水按照质量体积比为0.4g:100ml混合,常温下磁力搅拌至充分混合均匀,并以均质机在10/30MPa的压力下高压均质,得到稳定、澄清、透明的水溶型虾青素纳米乳液。该水溶型虾青素纳米乳液能够全部过500目筛,能够在常温下2年内不分层、降解和变质。
实施例6:
一种虾青素护肤霜膏的制备方法:
在40℃下,将实施例1制得的亲水性虾青素乳化分散油按照质量体积比为1~1.2g亲水性虾青素乳化分散油:100mL护肤霜膏的比例,加入到护肤霜中,具体是护肤霜膏最后的制备工艺中,常温磁力搅拌混合均匀,制成具有虾青素强抗氧、防晒功能的护肤霜膏。
实施例7:
本方案还包括虾青素油在食品中的应用。
一种虾青素饮料的制备方法:
将实施例3制得的亲水性虾看素乳化分散油按照质量体积比为0.6~0.8g:100mL的比例,加入到含乳蛋质为1.5%的火龙果汁乳饮料中,均质后得到均匀、稳定,具有清除自由基和抗氧化功能的火龙果汁乳饮料。
实施例8:
一种虾青素果蔬酱的制备方法:
将实施例4制得的亲水性虾看素乳化分散油按照质量体积比为1~2g:100mL的比例,加入到果蔬酱中,磁力搅拌,混合均匀,制成含虾青素抗氧化功能,稳定性更好的美味的果蔬酱。果蔬酱可以为西红柿酱、火龙果酱等。
性能检测:
一、亲水性虾青素乳化分散油的稳定性测试:
将从雨生红球藻中提取的虾青素提取的原始虾青素油作为对比例,将上述实施例2所制备的亲水性虾青素乳化分散油作为实验例,在60℃进行稳定性测试。图1是对比例和实验例在60℃保存不同时间后虾青素浓度变化图。
由图1可知,原始虾青素油的稳定性比较差,在60℃下,3个月后,有效的虾青素成分几乎氧化分解完,而亲水性虾青素乳化分散油仍能基本保持原本的含量,稳定性高。
二、实施例1-5的粒径测试;
采用马尔文粒径仪对实施例的产品进行粒径测试,测试结果见图2-6所示。
实施例1的亲水性虾青素乳化分散油的平均粒径为161.1nm;
实施例2的亲水性虾青素乳化分散油的平均粒径为136.8nm;
实施例3的亲水性虾青素乳化分散油的平均粒径为338nm;
实施例4的亲水性虾青素乳化分散油的平均粒径为287nm;
实施例5的虾青素乳液的平均粒径为217.7nm。
三、虾青素乳液的透明度测试:
采用透明度测定仪测定虾青素乳液的透明度:
试样制备:先将虾青素乳液作为被测试样用100目的铜滤网过滤,然后放置在一个50mL的烧杯中。取一个仪器自备的盛放被测试样的器皿,用与被测试样相应的溶剂将器皿内外清洗干净,然后用仪器自备的镜头纸将器皿外部擦拭干净,内部甩干,将制备好的被测试样仔细倒入清洗干净的器皿。液面高度不低于器皿的4/5,保证两透明面没有任何污痕,以备检测。
检测方法:仪器水平放置,先打开电源使仪器进行预热。盖好仪器上部测量口上盖,仔细调整校准仪器,使其显示值为100%。打开测量口上盖,***制备好的器皿,盖上上盖,读取此时的显示百分数,即为被测试样的透明度。
检测结果:虾青素乳液的透明度为99.6-99.8%。几乎等同于纯净水的透明度。
Claims (8)
1.一种亲水性虾青素乳化分散油的制备方法,其特征在于:包括以下制备步骤:
步骤一、将吐温和磷脂按照质量比为3:1~6:1混合,加热至50~60℃,搅拌至混合均匀,得复合乳化剂;
步骤二、将复合乳化剂和丙二醇按照质量比为5:1~9:1混合,加热至50~60℃,搅拌至充分混合均匀,得复合乳化稳定剂;
步骤三、将复合乳化稳定剂和虾青素含量为10%的虾青素油混合,加热至50~60℃,搅拌至充分混合均匀,降至常温,通过高压均质机超细化均质至纳米级,得亲水性虾青素乳化分散油。
2.根据权利要求1所述的一种亲水性虾青素乳化分散油的制备方法,其特征在于:步骤一的搅拌时间为5~10min,步骤二的搅拌时间为2~5min,步骤三的搅拌时间为1~2min。
3.根据权利要求1所述的一种亲水性虾青素乳化分散油的制备方法,其特征在于:所述步骤三中,复合乳化稳定剂与虾青素油的质量比为3:1~9:1。
4.根据权利要求1所述的一种亲水性虾青素乳化分散油的制备方法,其特征在于:所述吐温采用吐温系列的乳化剂,所述磷脂采用磷脂酰胆碱或胆碱磷脂的含量在25~50%的磷脂,所述丙二醇的纯度在99%以上。
5.根据权利要求1所述的一种亲水性虾青素乳化分散油的制备方法,其特征在于:所述吐温采用吐温80、吐温60、吐温40或者吐温20;所述磷脂采用大豆磷脂或者卵磷脂。
6.根据权利要求1所述的一种亲水性虾青素乳化分散油的制备方法,其特征在于:所述步骤三中的高压均质的压力为15/65MPpa~30/150MPa。
7.根据权利要求1~6任意一项权利要求所述的亲水性虾青素乳化分散油的应用,其特征在于:所述亲水性虾青素乳化分散油在制备化妆品上的应用,所述化妆品包括乳液。
8.根据权利要求1~6任意一项权利要求所述的亲水性虾青素乳化分散油的应用,其特征在于:所述亲水性虾青素乳化分散油在制备食品上的应用。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210773805.4A CN115227587B (zh) | 2022-07-01 | 2022-07-01 | 一种亲水性虾青素乳化分散油的制备方法及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210773805.4A CN115227587B (zh) | 2022-07-01 | 2022-07-01 | 一种亲水性虾青素乳化分散油的制备方法及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN115227587A CN115227587A (zh) | 2022-10-25 |
CN115227587B true CN115227587B (zh) | 2024-01-26 |
Family
ID=83671418
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210773805.4A Active CN115227587B (zh) | 2022-07-01 | 2022-07-01 | 一种亲水性虾青素乳化分散油的制备方法及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN115227587B (zh) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008154577A (ja) * | 2006-12-01 | 2008-07-10 | Fujifilm Corp | エマルション組成物、該エマルション組成物を含む食品及び化粧品 |
CN104352439A (zh) * | 2014-10-20 | 2015-02-18 | 河南牧翔动物药业有限公司 | 泰妙菌素脂质体及其制备方法和用途 |
CN104473878A (zh) * | 2014-11-20 | 2015-04-01 | 中国海洋大学 | 一种高水分散虾青素酯微球的制备方法 |
CN107812194A (zh) * | 2016-10-07 | 2018-03-20 | 福建奥正投资发展有限公司 | 亲水性的脂类组合物,其制备及调配方法和组合使用 |
CN109662900A (zh) * | 2019-02-25 | 2019-04-23 | 南京华狮新材料有限公司 | 一种磷脂包裹纳米乳组合物及其制备方法和应用 |
CN113966838A (zh) * | 2021-10-14 | 2022-01-25 | 恒枫食品科技有限公司 | 一种虾青素纳米结构脂质载体-壳聚糖凝胶微粒及制备方法 |
CN114948772A (zh) * | 2022-04-29 | 2022-08-30 | 可莱尼化妆品科技有限公司 | 一种虾青素纳米囊及其制备方法和应用 |
-
2022
- 2022-07-01 CN CN202210773805.4A patent/CN115227587B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2008154577A (ja) * | 2006-12-01 | 2008-07-10 | Fujifilm Corp | エマルション組成物、該エマルション組成物を含む食品及び化粧品 |
CN104352439A (zh) * | 2014-10-20 | 2015-02-18 | 河南牧翔动物药业有限公司 | 泰妙菌素脂质体及其制备方法和用途 |
CN104473878A (zh) * | 2014-11-20 | 2015-04-01 | 中国海洋大学 | 一种高水分散虾青素酯微球的制备方法 |
CN107812194A (zh) * | 2016-10-07 | 2018-03-20 | 福建奥正投资发展有限公司 | 亲水性的脂类组合物,其制备及调配方法和组合使用 |
CN109662900A (zh) * | 2019-02-25 | 2019-04-23 | 南京华狮新材料有限公司 | 一种磷脂包裹纳米乳组合物及其制备方法和应用 |
CN113966838A (zh) * | 2021-10-14 | 2022-01-25 | 恒枫食品科技有限公司 | 一种虾青素纳米结构脂质载体-壳聚糖凝胶微粒及制备方法 |
CN114948772A (zh) * | 2022-04-29 | 2022-08-30 | 可莱尼化妆品科技有限公司 | 一种虾青素纳米囊及其制备方法和应用 |
Also Published As
Publication number | Publication date |
---|---|
CN115227587A (zh) | 2022-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Mehmood et al. | Optimization of mixed surfactants-based β-carotene nanoemulsions using response surface methodology: An ultrasonic homogenization approach | |
Huang et al. | Nanostructured lipid carrier (NLC) as a strategy for encapsulation of quercetin and linseed oil: Preparation and in vitro characterization studies | |
Banasaz et al. | Encapsulation of lipid-soluble bioactives by nanoemulsions | |
Mayer et al. | Vitamin E-enriched nanoemulsions formed by emulsion phase inversion: factors influencing droplet size and stability | |
Hategekimana et al. | Vitamin E nanoemulsions by emulsion phase inversion: Effect of environmental stress and long-term storage on stability and degradation in different carrier oil types | |
DE69232376T2 (de) | Carotinoide zusammensetzung | |
CN110215416B (zh) | 一种山茶油油凝胶乳液及其制备方法 | |
DE102005031464A1 (de) | Emulgatorsystem, Emulsion und deren Verwendung | |
Chen et al. | Enhancement of the solubility and antioxidant capacity of α-linolenic acid using an oil in water microemulsion | |
CN105595158B (zh) | 一种具有着色、加香功能的饮料乳剂及其制备方法 | |
Zhang et al. | The formation, stability of DHA/EPA nanoemulsion prepared by emulsion phase inversion method and its application in apple juice | |
WO2007003599A2 (de) | Emulgatorsystem, emulsion und deren verwendung | |
CN107259506A (zh) | 一种β‑胡萝卜素纳米级乳剂的制备工艺及应用 | |
Zhong et al. | γ-Oryzanol nanoemulsions produced by a low-energy emulsification method: An evaluation of process parameters and physicochemical stability | |
JPWO2012005347A1 (ja) | 乳化組成物、その製造方法及びこれを含有する飲食品 | |
JPWO2018190203A1 (ja) | 酸化防止剤分散物 | |
Pascual‐Pineda et al. | Kinetic and thermodynamic stability of paprika nanoemulsions | |
CN112056558A (zh) | 一种水包油类胡萝卜素微乳液及其制备方法 | |
Chow et al. | Solid self-microemulsifying system (S-SMECS) for enhanced bioavailability and pigmentation of highly lipophilic bioactive carotenoid | |
Zhu et al. | Formation and stability of Eucommia ulmoides Oliver seed oil‐loaded inverse microemulsion formed by food‐grade ingredients and its antioxidant activities | |
Gao et al. | Novel strategy for enhancing the color intensity of β-Carotene: Enriching onto the oil-water interface | |
CN115227587B (zh) | 一种亲水性虾青素乳化分散油的制备方法及其应用 | |
Zhang et al. | Pickering emulsions stabilized by homogenized ball-milled eggshell particles in combination with sodium alginate | |
Lan et al. | Preparation and oxidation stability evaluation of tea polyphenols‐loaded inverse micro‐emulsion | |
Fernandes et al. | Effect of the use of ethanol and chia mucilage on the obtainment and techno‐functional properties of chia oil nanoemulsions |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |