CN115176862A - Microbial fermented feed prepared from Chinese herbal medicines and preparation method thereof - Google Patents
Microbial fermented feed prepared from Chinese herbal medicines and preparation method thereof Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a microbial fermented feed prepared from Chinese herbal medicines and a preparation method thereof, wherein the feed comprises the following raw materials in parts by weight: 30-40 parts of soybean meal, 15-20 parts of bran, 5-10 parts of corncob powder, 3-5 parts of sweet potato powder, 2-4 parts of soybean polypeptide, 1-3 parts of spirulina powder, 4-6 parts of Chinese herbal medicine leavening, 1-2 parts of astragalus extract, 1-2 parts of codonopsis pilosula extract, 0.3-0.6 part of composite trace elements and 0.5-1 part of composite zymocyte. The feed comprises meal, bran, corncob powder, sweet potato powder, soybean polypeptide, spirulina powder, chinese herbal medicine fermentation products, astragalus membranaceus extract, codonopsis pilosula extract, compound trace elements and the like, is reasonable in formula, comprehensive in nutrition, rich in nitrogen, phosphorus and potassium, protein, fat, cellulose and rich organic matters, provides enough nutrient substances for aquaculture, effectively improves meat quality and nutritional value of aquatic animals, and promotes growth of the aquatic animals.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a microbial fermentation feed compatible with Chinese herbal medicines and a preparation method thereof.
Background
In recent years, with the development of economy, the living standard of people is gradually improved, the demand of the whole society for protein in food is also gradually improved, with the development of fishery and animal husbandry, the supply of meat can basically meet the social demand, particularly, fish meat, which is white meat with low fat content, is popular with the masses in the market, but has certain distance from the expectations of people in terms of quality.
However, in the traditional breeding process, some problems exist more or less, for example, in the carp breeding process, the breeding concept of most breeding households is lagged behind, the feed management cannot be kept up with, many breeding households adopt low-quality materials, the energy and protein of the low-quality materials are often seriously insufficient, the growth speed of the carps is too slow, the feed conversion rate is low, the feeding period is long, and the economic benefit of the breeding households is seriously damaged; in the crucian carp breeding process, because the crucian carp fed by the concentrated feed has obviously reduced physique under the condition of high-density breeding, and the too high breeding density can generate stress, reduce the resistance of the fish to diseases, and cause low survival rate, and is easy to be damaged by gill rot, red skin disease, bleeding disease and enteritis in the breeding process, the four diseases are called 'four diseases of crucian carp', the outbreak of the diseases can bring devastating attack to the crucian carp breeding industry, and the breeding of the crucian carp is greatly restricted.
In addition, high density intensification is an important feature of current aquaculture. The high-density culture improves the feeding amount, increases the culture garbage in the water body and further deteriorates the water quality. The content of dissolved oxygen in water is reduced, and the content of ammonia nitrogen and nitrite exceeds the standard. These factors can cause oxidative damage to fish, thereby reducing the digestive absorption of feed by fish, reducing the growth and immune function of fish, and increasing the morbidity and mortality of fish. How to minimize these hazards while maintaining high density culture conditions. Then the quality of fish feed is a key factor influencing the water ecosystem, the disease resistance of fish and the quality of fish, how to improve the antibacterial and anti-inflammatory properties of the feed without adding antibiotics.
Based on the above, the application provides a microbial fermented feed compatible with Chinese herbal medicines and a preparation method thereof, so as to solve the problems in the prior art.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a microbial fermentation feed compatible with Chinese herbal medicines, which is prepared from the following raw materials in parts by weight: 30-40 parts of soybean meal, 15-20 parts of bran, 5-10 parts of corncob powder, 3-5 parts of sweet potato powder, 2-4 parts of soybean polypeptide, 1-3 parts of spirulina powder, 4-6 parts of Chinese herbal medicine leavening, 1-2 parts of astragalus extract, 1-2 parts of codonopsis pilosula extract, 0.3-0.6 part of composite trace elements and 0.5-1 part of composite zymocyte.
Preferably, the feed consists of the following raw materials in parts by weight: 35 parts of bean pulp, 18 parts of bran, 7.5 parts of corncob powder, 4 parts of sweet potato powder, 3 parts of soybean polypeptide, 2 parts of spirulina powder, 5 parts of Chinese herbal medicine fermentation product, 1.5 parts of astragalus extract, 1.5 parts of codonopsis pilosula extract, 0.5 part of composite trace element and 0.7 part of composite zymophyte.
Preferably, the Chinese herbal medicine fermentation product is prepared by mixing and fermenting houttuynia cordata, fructus viticis negundo, andrographis paniculata, schisandra chinensis, herba cirsii, lactobacillus and saccharomycetes.
Preferably, the Chinese herbal medicine fermentation product comprises the following raw materials in parts by weight: 15-20 parts of houttuynia cordata, 8-12 parts of negundo chastetree fruit, 5-7 parts of common andrographis herb, 7-12 parts of Chinese magnoliavine fruit, 3-5 parts of common thistle herb, 0.5-1 part of lactobacillus and 0.3-0.5 part of yeast.
Preferably, the preparation method of the Chinese herbal medicine fermentation product comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times volume of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, performing aerobic fermentation under stirring for 3-5 days, inoculating lactobacillus plantarum, standing under sealed condition, performing anaerobic fermentation for 5-7 days to obtain Chinese herbal fermented product.
Preferably, the preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times of 65% ethanol solution, extracting with 60-80kHz ultrasonic wave at room temperature for 2 times, each for 20min, filtering to obtain ethanol extract, adding 6-8 times of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire to concentrate the extract, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
Preferably, the preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 3-5 hr, repeatedly extracting once, mixing filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 15-17 hr, vacuum filtering, and washing the obtained precipitate with anhydrous ethanol and acetone sequentially to obtain radix Codonopsis extract.
Preferably, the composite trace elements consist of copper hydroxymethionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, and the mass ratio of the copper hydroxymethionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is (4).
Preferably, the composite zymophyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
The invention also aims to provide a preparation method of the microbial fermented feed compatible with the Chinese herbal medicines, which comprises the following steps:
(1) Weighing bean pulp, bran, corncob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating a mixed strain consisting of bacillus subtilis and candida in the mixture A, carrying out aerobic fermentation under the condition of stirring for 4-6 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 3-5 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with Chinese herbal medicine leavening, astragalus extract, codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
The invention has the following beneficial effects:
(1) The feed provided by the invention comprises meal, bran, corncob meal, sweet potato meal, soybean polypeptide, spirulina powder, chinese herbal medicine fermentation products, astragalus extract, codonopsis pilosula extract, composite trace elements and the like, is reasonable in formula, contains rich nitrogen, phosphorus and potassium in comprehensive nutrition, also contains protein, fat, cellulose and rich organic matters, provides enough nutrient substances for aquaculture, effectively improves the meat quality and the nutritional value of aquatic products, and promotes the growth of aquatic animals.
(2) The feed disclosed by the invention is added with various selected traditional Chinese medicinal material components, and the traditional Chinese medicinal material components are matched with the astragalus extract and the codonopsis pilosula extract, so that the feed has a good promotion effect on the growth and development of aquatic animals and the improvement of immunity, and can also play a role in preventing diseases. The heartleaf houttuynia herb, the negundo chastetree fruit, the common andrographis herb, the Chinese magnoliavine fruit and the common cephalanoplos herb are matched for use, so that the antibacterial and disease-resistant effects can be well achieved; the feed has certain fishy smell due to the matching of the soybean polypeptide and the spirulina powder, has a good food calling effect on fishes, is rich in various proteins and vitamins, can meet the nutritional requirements of the fishes, and is beneficial to improving the immunity of aquatic animals; meanwhile, by fermenting the houttuynia cordata, the negundo chastetree fruit, the common andrographis herb, the Chinese magnoliavine fruit and the common thistle herb, the nutritional ingredients in the houttuynia cordata, the negundo chastetree fruit, the common andrographis herb, the Chinese magnoliavine fruit and the common thistle herb can be effectively released, the palatability is improved, and the production of aquatic fishes is promoted.
Detailed Description
The following examples are provided to illustrate the technical solutions of the present invention more clearly, and should not be taken as limiting the scope of the present invention.
Example 1
The microbial fermented feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 30 parts of soybean meal, 15 parts of bran, 5 parts of corncob powder, 3 parts of sweet potato powder, 2 parts of soybean polypeptide, 1 part of spirulina powder, 4 parts of Chinese herbal medicine fermentation product, 1 part of astragalus extract, 1 part of codonopsis pilosula extract, 0.3 part of composite trace element and 0.5 part of composite zymocyte.
Wherein the Chinese herbal medicine fermentation product is prepared by mixing and fermenting houttuynia cordata, negundo chastetree fruit, common andrographis herb, chinese magnoliavine fruit, common thistle herb, lactobacillus and saccharomycetes.
Wherein the Chinese herbal medicine fermentation product comprises the following raw materials in parts by weight: 15 parts of heartleaf houttuynia herb, 8 parts of negundo chastetree fruit, 5 parts of common andrographis herb, 7 parts of Chinese magnoliavine fruit, 3 parts of common cephalanoplos herb, 0.5 part of lactobacillus and 0.3 part of microzyme.
The preparation method of the Chinese herbal medicine leavening comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times volume of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, performing aerobic fermentation under stirring for 3 days, inoculating lactobacillus plantarum, standing under sealed condition, performing anaerobic fermentation for 5 days to obtain Chinese herbal fermented product.
The preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times weight of 65% ethanol solution, extracting with 60kHz ultrasonic wave at room temperature for 2 times, each time for 20min, filtering to obtain ethanol extract, adding 6-8 times weight of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire, concentrating, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
The preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 3 hr, extracting repeatedly, mixing filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 15 hr, vacuum filtering, and washing the obtained precipitate with anhydrous ethanol and acetone sequentially to obtain radix Codonopsis extract.
The composite trace elements comprise copper hydroxy methionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxy methionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is 4.
Wherein the composite zymocyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed compatible with the Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corn cob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating mixed strains composed of bacillus subtilis and candida, carrying out aerobic fermentation under the condition of stirring for 4 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 3 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with Chinese herbal medicine leavening, astragalus extract, codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Example 2
The microbial fermentation feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 40 parts of soybean meal, 20 parts of bran, 10 parts of corncob powder, 5 parts of sweet potato powder, 4 parts of soybean polypeptide, 3 parts of spirulina powder, 6 parts of Chinese herbal medicine fermentation product, 2 parts of astragalus extract, 2 parts of codonopsis pilosula extract, 0.6 part of composite trace element and 1 part of composite zymocyte.
Wherein the Chinese herbal medicine fermentation product is prepared by mixing and fermenting houttuynia cordata, negundo chastetree fruit, common andrographis herb, chinese magnoliavine fruit, common thistle herb, lactobacillus and saccharomycetes.
The Chinese herbal medicine fermentation product comprises the following raw materials in parts by weight: 20 parts of houttuynia cordata, 8-12 parts of negundo chastetree fruit, 7 parts of common andrographis herb, 12 parts of Chinese magnoliavine fruit, 5 parts of common cephalanoplos herb, 1 part of lactobacillus and 0.5 part of microzyme.
The preparation method of the Chinese herbal medicine leavening comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times volume of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, aerobic fermenting under stirring for 5 days, inoculating lactobacillus plantarum, standing under sealed condition, anaerobic fermenting for 7 days to obtain Chinese herbal fermented product.
The preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times of 65% ethanol solution, extracting with 80kHz ultrasonic wave at room temperature for 2 times, each for 20min, filtering to obtain ethanol extract, adding 6-8 times of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire to concentrate the extract, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
The preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 5 hr, repeating extraction once, mixing the two filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 17 hr, vacuum filtering, washing the obtained precipitate with anhydrous ethanol and acetone acid sequentially to obtain radix Codonopsis extract.
The composite trace elements comprise copper hydroxy methionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxy methionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is 4.
Wherein the composite zymocyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed with compatibility of Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corncob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating a mixed strain consisting of bacillus subtilis and candida in the mixture A, carrying out aerobic fermentation under the condition of stirring for 6 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 5 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with Chinese herbal medicine leavening, astragalus extract, codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Example 3
The microbial fermentation feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 35 parts of soybean meal, 18 parts of bran, 7.5 parts of corncob powder, 4 parts of sweet potato powder, 3 parts of soybean polypeptide, 2 parts of spirulina powder, 5 parts of Chinese herbal medicine leavening, 1.5 parts of astragalus extract, 1.5 parts of codonopsis pilosula extract, 0.5 part of composite trace elements and 0.7 part of composite zymophyte.
Wherein the Chinese herbal medicine fermentation product is prepared by mixing and fermenting houttuynia cordata, negundo chastetree fruit, common andrographis herb, chinese magnoliavine fruit, common thistle herb, lactobacillus and saccharomycetes.
The Chinese herbal medicine fermentation product comprises the following raw materials in parts by weight: 18 parts of heartleaf houttuynia herb, 10 parts of negundo chastetree fruit, 6 parts of common andrographis herb, 10 parts of Chinese magnoliavine fruit, 3-5 parts of common cephalanoplos herb, 0.7 part of lactobacillus and 0.4 part of microzyme.
The preparation method of the Chinese herbal medicine leavening comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, performing aerobic fermentation under stirring for 4 days, inoculating lactobacillus plantarum, standing under sealed condition, performing anaerobic fermentation for 6 days to obtain Chinese herbal fermented product.
The preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times weight of 65% ethanol solution, extracting with 70kHz ultrasonic wave at room temperature for 2 times (20 min each time), filtering to obtain ethanol extract, adding 6-8 times weight of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire, concentrating, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
The preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 4 hr, extracting repeatedly, mixing filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 16 hr, vacuum filtering, and washing the obtained precipitate with anhydrous ethanol and acetone sequentially to obtain radix Codonopsis extract.
The composite trace elements comprise copper hydroxy methionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxy methionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is 4.
Wherein the composite zymophyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed compatible with the Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corncob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating mixed strains composed of bacillus subtilis and candida, carrying out aerobic fermentation under the condition of stirring for 5 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 4 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with Chinese herbal medicine leavening, astragalus extract, codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Example 4
The microbial fermentation feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 33 parts of soybean meal, 16 parts of bran, 6 parts of corn cob powder, 3.5 parts of sweet potato powder, 2.5 parts of soybean polypeptide, 1.5 parts of spirulina powder, 4.5 parts of Chinese herbal medicine leavening, 1.3 parts of astragalus extract, 1.3 parts of codonopsis pilosula extract, 0.4 part of composite trace elements and 0.6 part of composite zymophyte.
Wherein the Chinese herbal medicine fermentation product is prepared by mixing and fermenting houttuynia cordata, negundo chastetree fruit, common andrographis herb, chinese magnoliavine fruit, common thistle herb, lactobacillus and saccharomycetes.
Wherein the Chinese herbal medicine fermentation product comprises the following raw materials in parts by weight: 16 parts of heartleaf houttuynia herb, 9 parts of negundo chastetree fruit, 5.5 parts of common andrographis herb, 8 parts of Chinese magnoliavine fruit, 3.5 parts of common cephalanoplos herb, 0.6 part of lactobacillus and 0.3 part of microzyme.
The preparation method of the Chinese herbal medicine leavening comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times volume of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, aerobic fermenting under stirring for 3 days, inoculating lactobacillus plantarum, standing under sealed condition, anaerobic fermenting for 5.5 days to obtain Chinese herbal fermented product.
The preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times of 65% ethanol solution, extracting with 65kHz ultrasonic wave at room temperature for 2 times (20 min each time), filtering to obtain ethanol extract, adding 6-8 times of hot water into the residue, decocting at 70 deg.C for 1.5h, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire, concentrating, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
The preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 3.5h, repeatedly extracting for one time, mixing the two filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until the ethanol content in the concentrated filtrate is 70-80%, standing for 15.5h, vacuum filtering, and washing the obtained precipitate with anhydrous ethanol and acetone sequentially to obtain radix Codonopsis extract.
The composite trace elements comprise copper hydroxy methionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxy methionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is 4.
Wherein the composite zymophyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed with compatibility of Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corn cob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating mixed strains composed of bacillus subtilis and candida, carrying out aerobic fermentation under the condition of stirring for 4.5 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 3.5 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with Chinese herbal medicine leavening, astragalus extract, codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Example 5
The microbial fermentation feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 30-40 parts of soybean meal, 19 parts of bran, 9 parts of corncob powder, 4.5 parts of sweet potato powder, 3.5 parts of soybean polypeptide, 2.5 parts of spirulina powder, 5.5 parts of Chinese herbal medicine leavening, 1.8 parts of astragalus extract, 1.7 parts of codonopsis pilosula extract, 0.5 part of composite trace elements and 0.9 part of composite zymocyte.
Wherein the Chinese herbal medicine fermentation product is prepared by mixing and fermenting houttuynia cordata, negundo chastetree fruit, common andrographis herb, chinese magnoliavine fruit, common thistle herb, lactobacillus and saccharomycetes.
Wherein the Chinese herbal medicine fermentation product comprises the following raw materials in parts by weight: 19 parts of heartleaf houttuynia herb, 11 parts of negundo chastetree fruit, 6.5 parts of common andrographis herb, 11 parts of Chinese magnoliavine fruit, 4.5 parts of common cephalanoplos herb, 0.8 part of lactobacillus and 0.5 part of microzyme.
The preparation method of the Chinese herbal medicine leavening comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, performing aerobic fermentation under stirring for 4.5 days, inoculating lactobacillus plantarum, standing under sealed condition, performing anaerobic fermentation, and fermenting for 6.5 days to obtain Chinese herbal fermented product.
The preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times of 65% ethanol solution, extracting with 75kHz ultrasonic wave at room temperature for 20min for 2 times, filtering to obtain ethanol extract, adding 6-8 times of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with the ethanol extract, heating with 100 deg.C direct fire, concentrating, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
The preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 4.5 hr, repeatedly extracting once, mixing filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 17 hr, vacuum filtering, and washing the obtained precipitate with anhydrous ethanol and acetone sequentially to obtain radix Codonopsis extract.
The composite trace elements comprise copper hydroxy methionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxy methionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is 4.
Wherein the composite zymophyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed compatible with the Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corncob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating a mixed strain consisting of bacillus subtilis and candida in the mixture A, carrying out aerobic fermentation under the condition of stirring for 5.5 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 4.5 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with Chinese herbal medicine leavening, astragalus extract, codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Comparative example 1
The microbial fermentation feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 35 parts of bean pulp, 18 parts of bran, 7.5 parts of corncob powder, 4 parts of sweet potato powder, 3 parts of soybean polypeptide, 2 parts of spirulina powder, 1.5 parts of astragalus extract, 1.5 parts of codonopsis pilosula extract, 0.5 part of composite trace elements and 0.7 part of composite zymophyte.
The preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times of 65% ethanol solution, extracting with 70kHz ultrasonic wave at room temperature for 2 times, each for 20min, filtering to obtain ethanol extract, adding 6-8 times of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire to concentrate the extract, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
The preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 4 hr, extracting repeatedly, mixing filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 16 hr, vacuum filtering, and washing the obtained precipitate with anhydrous ethanol and acetone sequentially to obtain radix Codonopsis extract.
The composite trace element is composed of copper hydroxymethionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxymethionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is (4).
Wherein the composite zymocyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed compatible with the Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corncob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating mixed strains composed of bacillus subtilis and candida, carrying out aerobic fermentation under the condition of stirring for 5 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 4 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with the astragalus extract, the codonopsis pilosula extract and the compound trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Comparative example 2
Except that the Chinese herbal medicine leavening is replaced by the Chinese herbal medicine mixture, the other parts are as follows:
the microbial fermentation feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 35 parts of soybean meal, 18 parts of bran, 7.5 parts of corncob powder, 4 parts of sweet potato powder, 3 parts of soybean polypeptide, 2 parts of spirulina powder, 5 parts of a Chinese herbal medicine mixture, 1.5 parts of astragalus extract, 1.5 parts of codonopsis pilosula extract, 0.5 part of composite trace elements and 0.7 part of composite zymocyte.
Wherein the Chinese herbal medicine mixture is prepared by mixing and fermenting houttuynia cordata, negundo chastetree fruit, andrographis paniculata, schisandra chinensis, setose thistle, lactobacillus and saccharomycetes.
The Chinese herbal medicine mixture comprises the following raw materials in parts by weight: 18 parts of heartleaf houttuynia herb, 10 parts of negundo chastetree fruit, 6 parts of common andrographis herb, 10 parts of Chinese magnoliavine fruit, 3-5 parts of common cephalanoplos herb, 0.7 part of lactobacillus and 0.4 part of microzyme.
The preparation method of the Chinese herbal medicine mixture comprises the following steps: weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture.
The preparation method of the astragalus extract comprises the following steps: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times of 65% ethanol solution, extracting with 70kHz ultrasonic wave at room temperature for 2 times, each for 20min, filtering to obtain ethanol extract, adding 6-8 times of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire to concentrate the extract, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
The preparation method of the codonopsis pilosula extract comprises the following steps: pulverizing radix Codonopsis, decocting in water for 4 hr, repeating extraction once, mixing the two filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 16 hr, vacuum filtering, washing the obtained precipitate with anhydrous ethanol and acetone acid sequentially to obtain radix Codonopsis extract.
The composite trace element is composed of copper hydroxymethionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxymethionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is (4).
Wherein the composite zymocyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed compatible with the Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corncob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating a mixed strain consisting of bacillus subtilis and candida in the mixture A, carrying out aerobic fermentation under the condition of stirring for 5 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 4 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with a Chinese herbal medicine mixture, an astragalus extract, a codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Comparative example 3
The microbial fermentation feed compatible with Chinese herbal medicines comprises the following raw materials in parts by weight: 35 parts of soybean meal, 18 parts of bran, 7.5 parts of corncob powder, 4 parts of sweet potato powder, 3 parts of soybean polypeptide, 2 parts of spirulina powder, 5 parts of Chinese herbal medicine leavening, 0.5 part of composite trace elements and 0.7 part of composite zymophyte.
Wherein the Chinese herbal medicine fermentation product is prepared by mixing and fermenting houttuynia cordata, negundo chastetree fruit, andrographis paniculata, schisandra chinensis, setose thistle, lactobacillus and saccharomycetes.
The Chinese herbal medicine fermentation product comprises the following raw materials in parts by weight: 18 parts of heartleaf houttuynia herb, 10 parts of negundo chastetree fruit, 6 parts of common andrographis herb, 10 parts of Chinese magnoliavine fruit, 3-5 parts of common cephalanoplos herb, 0.7 part of lactobacillus and 0.4 part of microzyme.
The preparation method of the Chinese herbal medicine leavening comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times volume of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, performing aerobic fermentation under stirring for 4 days, inoculating lactobacillus plantarum, standing under sealed condition, performing anaerobic fermentation for 6 days to obtain Chinese herbal fermented product.
The composite trace element is composed of copper hydroxymethionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, wherein the mass ratio of the copper hydroxymethionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is (4).
Wherein the composite zymophyte is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
In addition, the preparation method of the microbial fermented feed with compatibility of Chinese herbal medicines comprises the following steps:
(1) Weighing bean pulp, bran, corn cob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating a mixed strain consisting of bacillus subtilis and candida in the mixture A, carrying out aerobic fermentation under the condition of stirring for 5 days, then inoculating lactobacillus plantarum, carrying out standing anaerobic fermentation under the condition of sealing, and fermenting for 4 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder, the mixture C, the Chinese herbal medicine leavening and the composite trace elements in the step (3), and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
Examples of the experiments
Taking 9 crucian culture net cages which are put in the same water area and cultured in the same batch, culturing 80 tails of crucians in each net cage, randomly dividing the cages into 9 groups, wherein the group 1 is a blank control group, and feeding the crucian granular feed sold in the market; groups 2-6 were test groups, and the feeds fed were the feeds prepared in examples 1-5, respectively; groups 7-9 are control groups fed with the feed prepared in comparative examples 1-3. Harvesting after feeding for 60 days (namely the whole experiment lasts for 60 days), randomly fishing 4 tails from each group before feeding, weighing the initial weight of the tail fin, leaving a corresponding label on the tail fin, continuously weighing the weight of the survival marked fish after the feeding experiment is finished, representing the average weight increase condition of each group of crucian, and simultaneously recording the death rate of each group of crucian in the feeding process, wherein the specific results are as follows:
group 1: the average weight gain of the blank control group is 225.5%, the mortality rate of 6 grass carps dead in the net cage is 7.5%, and the marked grass carps do not die. The group of grass carp individuals is small as a whole.
Group 2: in the group of example 1, the average weight gain rate is 298.3%, and the cage death rate of 1 tail of the grass carp is 1.25%.
Group 3: example 2 group, average rate of weight gain 323.2%, during which time the cage was the grass carp dead, no grass carp death was marked.
Group 4: example 3 group, mean weight gain 344.6%, during which time the cage was the onset of grass carp death, marked no grass carp death.
Group 5: example 4 group, mean weight gain 335.3% during which time the cage was the death of grass carp, marking no death of grass carp.
Group 6: example 5 group, mean weight gain 333.1%, during which time the cage was the death of grass carp, marked no death of grass carp.
Group 7: in the comparative example 1 group, the average weight gain rate was 248.1%, during which the cage-killed 3-fish grass carps had a mortality rate of 3.75%, and no death occurred in the labeled grass carps.
Group 8: in the comparative example 2 group, the average weight gain rate was 267.7%, during which the cage killed 1 tail of the grass carps, the mortality rate was 1.25%, and no death occurred in the labeled grass carps.
Group 9: comparative example 3 group, mean weight gain 277.4%, during which 1 tail of the cage-dead grass carp died, mortality was 1.25%, and no death occurred in the labeled grass carp.
Wherein the mean rate of weight gain = (total end of test total weight of tagged fish-initial total weight of tagged fish)/initial total weight 100%.
The test results show that the product of the invention can effectively promote the growth of crucian, the average weight gain rate of the product is obviously higher than that of a blank control group, a group 7 (comparative example 1), a group 8 (comparative example 2) and a group 9 (comparative example 3), and the invention proves that the raw materials in the formula of the invention have a synergistic effect and are essential for achieving the technical effect. When the product of the invention is used for feeding, the death rate of crucian carps is reduced compared with that of the group 1 and the group 7-9, and the result proves that the Chinese herbal medicines in the feed have the synergistic effect, and the synergistic effect is further improved after fermentation treatment, so that the immunity of the crucian carps can be effectively improved, and the disease resistance of the crucian carps is improved.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or that equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The microbial fermented feed compatible with Chinese herbal medicines is characterized by comprising the following raw materials in parts by weight: 30-40 parts of soybean meal, 15-20 parts of bran, 5-10 parts of corncob powder, 3-5 parts of sweet potato powder, 2-4 parts of soybean polypeptide, 1-3 parts of spirulina powder, 4-6 parts of Chinese herbal medicine leavening, 1-2 parts of astragalus extract, 1-2 parts of codonopsis pilosula extract, 0.3-0.6 part of composite trace elements and 0.5-1 part of composite zymocyte.
2. The microbial fermented feed compatible with Chinese herbal medicines according to claim 1, wherein the feed is prepared from the following raw materials in parts by weight: 35 parts of soybean meal, 18 parts of bran, 7.5 parts of corncob powder, 4 parts of sweet potato powder, 3 parts of soybean polypeptide, 2 parts of spirulina powder, 5 parts of Chinese herbal medicine leavening, 1.5 parts of astragalus extract, 1.5 parts of codonopsis pilosula extract, 0.5 part of composite trace elements and 0.7 part of composite zymophyte.
3. The microbial fermented feed compatible with Chinese herbal medicines according to claim 1, wherein the Chinese herbal medicine fermented product is prepared by mixing and fermenting herba houttuyniae, fructus viticis negundo, herba andrographitis, fructus schizandrae, herba cirsii jeponici, lactobacillus and yeast.
4. The microbial fermented feed compatible with Chinese herbal medicines according to claim 3, wherein the Chinese herbal medicine fermented product comprises the following raw materials in parts by weight: 15-20 parts of houttuynia cordata, 8-12 parts of negundo chastetree fruit, 5-7 parts of common andrographis herb, 7-12 parts of Chinese magnoliavine fruit, 3-5 parts of common thistle herb, 0.5-1 part of lactobacillus and 0.3-0.5 part of yeast.
5. The microbial fermented feed compatible with Chinese herbal medicines according to claim 4, wherein the preparation method of the Chinese herbal medicine fermented product comprises the following steps:
(1) Diluting lactobacillus and yeast with 20-30 times volume of water to obtain diluted fermentation strain liquid;
(2) Weighing herba Houttuyniae, fructus Viticis negundo, herba Andrographitis, fructus Schisandrae chinensis, and herba Cirsii Japonici, mixing, and pulverizing to 80-100 mesh to obtain Chinese herbal mixture;
(3) Inoculating the Chinese herbal mixture into mixed strain composed of lactobacillus and yeast, aerobic fermenting under stirring for 3-5 days, inoculating lactobacillus plantarum, standing under sealed condition, anaerobic fermenting for 5-7 days to obtain Chinese herbal fermented product.
6. The microbial fermented feed compatible with Chinese herbal medicines according to claim 1, wherein the astragalus extract is prepared by the following method: pulverizing radix astragali, sieving with 30-40 mesh sieve, adding 6-8 times of 65% ethanol solution, extracting with 60-80kHz ultrasonic wave at room temperature for 2 times, each for 20min, filtering to obtain ethanol extract, adding 6-8 times of hot water into the residue, decocting at 70 deg.C for 1.5 hr, mixing the filtrate with ethanol extract, heating with 100 deg.C direct fire to concentrate the extract, and drying by conventional method. Pulverizing, and sieving with 50 mesh sieve to obtain radix astragali extract.
7. The microbial fermented feed compatible with Chinese herbal medicines according to claim 1, wherein the codonopsis pilosula extract is prepared by the following method: pulverizing radix Codonopsis, decocting in water for 3-5 hr, repeatedly extracting once, mixing filtrates, filtering again, concentrating the filtrate by rotary evaporation to 5-10% of the original volume, adding ethanol until ethanol content in the concentrated filtrate is 70-80%, standing for 15-17 hr, vacuum filtering, and washing the obtained precipitate with anhydrous ethanol and acetone sequentially to obtain radix Codonopsis extract.
8. The microbial fermented feed compatible with Chinese herbal medicines according to claim 1, wherein the composite trace elements consist of copper hydroxymethionine, zinc methionine, manganese methionine, cobalt methionine and selenium methionine, and the mass ratio of the copper hydroxymethionine, the zinc methionine, the manganese methionine, the cobalt methionine and the selenium methionine is 4.
9. The microbial fermented feed compatible with Chinese herbal medicines according to claim 1, wherein the composite fermentation bacteria is a composite strain consisting of bacillus subtilis, lactobacillus plantarum and candida, and the weight ratio of the bacillus subtilis to the lactobacillus plantarum to the candida is 3.
10. The method for preparing the microbial fermented feed compatible with Chinese herbal medicines according to any one of claims 1 to 9, comprising the steps of:
(1) Weighing bean pulp, bran, corncob powder, sweet potato powder, soybean polypeptide and spirulina powder, mixing, and then crushing to 40-60 meshes to obtain a mixture A;
(2) Inoculating mixed strains composed of bacillus subtilis and candida, performing aerobic fermentation under the condition of stirring for 4-6 days, inoculating lactobacillus plantarum, performing standing anaerobic fermentation under the condition of sealing, and performing fermentation for 3-5 days to obtain a mixture B;
(3) Filtering the mixture B to obtain a filtrate containing thalli and filter residue, performing vacuum freeze drying on the filtrate to obtain bacterial powder, drying the filter residue in a hot air drying mode to ensure that the water content is lower than 5%, crushing the dried filter residue to 80-100 meshes to obtain a mixture C;
(4) And (4) mixing the bacterial powder and the mixture C in the step (3) with Chinese herbal medicine leavening, astragalus extract, codonopsis pilosula extract and composite trace elements, and uniformly stirring to obtain the microbial fermented feed compatible with the Chinese herbal medicines.
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| CN105166553A (en) * | 2015-09-28 | 2015-12-23 | 佛山市高明区杨和镇金峰水产养殖场 | Shrimp feed and producing method thereof |
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