CN115068361B - 一种蓝铜肽双剂及其使用方法和应用 - Google Patents
一种蓝铜肽双剂及其使用方法和应用 Download PDFInfo
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Abstract
本发明涉及A61K,更具体地,本发明涉及一种蓝铜肽双剂及其使用方法和应用。包括:肽剂,所述肽剂包括三肽‑1;铜剂,所述铜剂包括铜盐。本发明通过分别将三肽‑1和铜盐制成肽剂和铜剂,在使用时再混合,可避免三肽‑1和铜盐得到的蓝铜肽的活性下降,从而当使用时,保证蓝铜肽的活性在双剂精华混合之前并未下降,可充分发挥本发明双剂对皮肤的修复和美白作用,促进皱纹减少,提高皮肤光泽度、白度,且本发明提供的双剂可不添加常规的防腐成分,通过少量多元醇和对羟基苯乙酮等皮肤调理剂来调节双剂的防腐效果,且本发明提供的双剂可用于敏感肌,促进对受损皮肤的修复以及退红,促进皮肤弹性和紧致性的提高。
Description
技术领域
本发明涉及A61K,更具体地,本发明涉及一种蓝铜肽双剂及其使用方法和应用。
背景技术
GHK三肽-1为人体天然存在物质,具有小的分子量,可有效结合铜离子,得到蓝铜肽作用于细胞中,促进皮肤的再生以及蛋白的生成,提高肌肤弹性和修复损伤。
CN110237007A公开一种含有蓝铜肽的化妆品组合物及其制备方法和应用,通过蓝铜肽、马齿苋提取物、积雪草提取物、VB5共同作用具有好的舒缓抗敏功效。
但三肽-1以及蓝铜肽稳定性较差,长期存放或者光照下容易发现水解,且在酸性环境中会出现解络合等问题,影响了蓝铜肽的活性,目前一般通过添加较多的防腐剂来提高存储性能,但防腐剂的添加容易带来敏感性等问题,故需要提供一种蓝铜肽的制剂和使用方法,来提高存放和使用性能。
发明内容
为了解决上述问题,本发明第一个方面提供了一种蓝铜肽双剂,包括:
肽剂,所述肽剂包括三肽-1;
铜剂,所述铜剂包括铜盐;
肽剂和铜剂分别制备。
三肽-1具有好的抗氧性,有利于蛋白的表达和生成,而铜离子也对人体内胶原蛋白和弹力蛋白的构建,以及透明质酸的生成等具有积极作用,当三肽-1和铜离子接触时,可快速得到小分子的三肽-1和铜的络合物,具有高的活性,但是长期储存容易失活,而本发明通过使用双剂的形式,在使用时再混合得到蓝铜肽,可充分发挥蓝铜肽活性,避免失活。
作为本发明一种优选的技术方案,所述三肽-1占肽剂的重量百分数大于等于0.2%。
作为本发明一种优选的技术方案,所述三肽-1占肽剂的0.2~0.6%,可列举的有,0.2%、0.3%、0.4%、0.5%、0.6%;优选为0.4~0.5%。
作为本发明一种优选的技术方案,所述铜盐选自乙酸铜、天冬氨酸铜、醋酸铜、乙酰丙酮酸铜、氯化铜、吡咯烷酮羧酸铜(PCA-Cu)、硫酸铜、葡糖酸铜、乙酰蛋氨酸铜、孔雀石提取物、孔雀石、叶绿素铜、多肽铜盐酸盐的一种或多种。
作为本发明一种优选的技术方案,所述铜盐占铜剂的重量百分数大于等于0.6%,可列举的有,0.6%、0.7%、0.75%、0.8%、0.85%、0.9%、0.95%、1%、1.05%、1.1%;优选为0.9~1%。
作为本发明一种优选的技术方案,所述肽剂的溶剂包括酵母发酵产物滤液和水,所述铜剂的溶剂包括酵母发酵产物滤液和水。
作为本发明一种优选的技术方案,所述酵母发酵产物滤液选自稻米发酵产物滤液、二裂酵母发酵产物滤液、半乳糖酵母样菌发酵产物滤液、酵母菌发酵产物滤液、裂殖酵母菌丝体发酵产物滤液、乳酸杆菌/豆浆发酵产物滤液、芽孢杆菌/谷氨酸发酵产物滤液中的一种或多种,优选为半乳糖酵母样菌发酵产物滤液。
此外,发明人也发现,肽类物质,如三肽-1的稳定性较差,也会影响三肽-1的稳定性,进而使得后续和铜剂反应,得到蓝铜肽的活性降低,而发明人发现,当制备肽剂时,通过使用酵母发酵产物滤液和水共同作为溶剂,在长期存储,或者受到热、光照等作用时,可在不使用防腐剂的同时,避免三肽-1的分解等作用,从而当存储较长时间后使用时,仍可快速和铜剂反应,得到蓝色的制剂,用于皱纹等的改善,且发明人还发现,还有利于促进皮肤提亮和美白的实现。
作为本发明一种优选的技术方案,所述肽剂中酵母发酵产物滤液的重量百分数为25~35%,可列举的有,25%、28%、30%、32%、35%;所述铜剂中酵母发酵产物滤液的重量百分数为25~35%,可列举的有,25%、28%、30%、32%、35%。
作为本发明一种优选的技术方案,所述铜剂还包括吸附剂,所述吸附剂选自环糊精、海藻酸钠、季铵盐中的一种或多种。在一种实施方式中,所述铜剂中吸附剂的重量百分数为1~2%,更优选为1~1.5%。
且发明人发现,在三肽-1和铜盐络合过程中,若铜盐的浓度较高,其会对铜剂中物质产生催化作用,尤其是受到光照等作用,可能产生氢氧自由基等,影响铜剂的稳定性,而本发明通过在铜剂添加吸附剂,如环糊精等,利用环糊精内腔疏水、外壁亲水的特性,在吸附二价铜阳离子的同时,也减少贮存过程中铜阳离子和其他成分,如发酵产物滤液、调节剂等的作用造成的活性下降,以及对后续蓝铜肽活性的影响,促进后续得到的双剂用于皮肤时,对皮肤紧致、弹性,以及保水性的提高。
作为本发明一种优选的技术方案,所述增稠剂选自产碱杆菌多糖类、黄原胶、卡波姆、丙烯酸(酯)类/C10-30烷醇丙烯酸酯交联聚合物、羟乙基纤维素中的一种或多种,不做具体限定。
作为本发明一种优选的技术方案,所述肽剂中增稠剂的重量百分数为0.1~1%,铜剂中增稠剂的重量百分数为0.1~1%,其中肽剂和铜剂中增稠剂可相同或不同,且可使用一种、两种或多种。作为一种实例,肽剂中包括重量百分数为0.2~0.5%黄原胶、0.1~0.5%产碱杆菌多糖类,铜剂中包括重量百分数为0.1~0.5%黄原胶、0.1~0.5%产碱杆菌多糖类。
作为本发明一种优选的技术方案,所述保湿剂包括多元醇类保湿剂、聚醚类保湿剂、多糖类保湿剂、氨基酸类保湿剂中的一种或多种。作为多元醇类保湿剂的实例,包括但不限于,丁二醇、丙三醇、1,2-戊二醇、聚丙二醇、甘油聚醚-26、山梨醇、2-甲基-1,3丙二醇、异戊二醇等,作为多糖类保湿剂的实例,包括但不限于,出芽短梗酶多糖、透明质酸钠、银耳多糖、木糖醇基葡糖苷、脱水木糖醇、木糖醇等,作为聚醚类保湿剂的实例,包括但不限于,甘油聚氧乙烯醚、聚氧丙烯醚、聚氧乙烯-聚氧丙烯共聚物等。
作为本发明一种优选的技术方案,所述肽剂中保湿剂的重量百分数为10~25%,铜剂中保湿剂的重量百分数为10~25%。
作为本发明一种优选的技术方案,所述保湿剂包括多元醇类保湿剂、多糖类保湿剂。作为本发明一种优选的技术方案,所述肽剂中多元醇类保湿剂的重量百分数为5~20%、聚醚类保湿剂1~3%、多糖类保湿剂的重量百分数为0.1~1%,铜剂中多元醇类保湿剂的重量百分数为5~20%、多糖类保湿剂的重量百分数为0.5~2%。
作为本发明一种优选的技术方案,所述肽剂中皮肤调理剂的重量百分数为1~4%,铜剂中皮肤调理剂的重量百分数为1~4%。
作为皮肤调理剂的实例,包括但不限于,烟酰胺、泛醇、积雪草(CENTELLAASIATICA)提取物、出芽短梗酶多糖、九肽-1、尿囊素、氢化卵磷脂、牛油果树果脂、神经酰胺3、燕麦(AVENA SATIVA)仁油、1,2-己二醇、对羟基苯乙酮等。本发明肽剂和铜剂中皮肤调理剂可相同或不同,也可选择一种或多种,不做具体限定。
作为本发明一种优选的技术方案,所述肽剂还包括润肤剂、抗氧剂、pH调节剂、乳化剂中的至少一种;所述铜剂还包括润肤剂、抗氧剂、pH调节剂、乳化剂中的至少一种。
作为润肤剂的实例,包括但不限于,牛油果树果油、鲸蜡硬脂醇、异壬酸异壬酯、辛酸/葵酸三甘油脂、橄榄油、霍霍巴籽油、角鲨烷、霍霍巴油、乳木果油、琥珀酸二乙氧基乙酯等。在一种实施方式中,所述肽剂中润肤剂的重量百分数为0.5~1%,所述铜剂中润肤剂的重量百分数为1~1.5%。
作为pH调节剂的实例,包括但不限于,三乙醇胺、月桂胺、氢氧化钠、氨甲基丙醇、乙二胺、碳酸氢钠等。在一种实施方式中,所述铜剂中pH调节剂的重量百分数为0.1~0.4%。
本发明提供的肽剂和铜剂为通过本领域熟知的混合、均质制备得到,不做具体限定。
本发明第二个方面提供了一种所述的蓝铜肽双剂的使用方法,包括:将铜剂和肽剂混合,发生变色反应。所述混合中,铜剂和肽剂的重量比为1:1~3,不做具体限定。
本发明第三个方面提供了一种蓝铜肽双剂的应用,用于护肤品。
本发明与现有技术相比具有以下有益效果:
本发明通过分别将三肽-1和铜盐制成肽剂和铜剂,在使用时再混合,可避免三肽-1和铜盐得到的蓝铜肽的活性下降,从而当使用时,保证蓝铜肽的活性在双剂精华混合之前并未下降,可充分发挥本发明双剂对皮肤的修复和美白作用,促进皱纹减少,提高皮肤光泽度、白度,且本发明提供的双剂可不添加常规的防腐成分,通过少量多元醇和对羟基苯乙酮等皮肤调理剂来调节双剂的防腐效果,且本发明提供的双剂可用于敏感肌,促进对受损皮肤的修复以及退红,促进皮肤弹性和紧致性的提高。此外,本发明提供的铜剂和肽剂混合时发生变色反应,快速得到变色后溶液,可更直观指示蓝铜肽合成,且本发明提供的铜剂和肽剂本身具有好的存储稳定性,进一步发挥蓝铜肽的作用。
附图说明
图1为功效测试方法。
图2为功效测试统计方法和结果。
图3为皮肤泛红缓解结果。
图4为皮肤颜色(脸颊)测试结果的一种实例。
图5为皮肤颜色(脸颊)测试结果的一种实例。
图6为皮肤颜色(脸颊)测试结果的一种实例。
图7为皮肤皱纹(Visia CR外眼角)测试结果。
图8为皮肤皱纹(Visia CR鼻唇沟)测试结果。
图9为皮肤皱纹(Primos CR外眼角)测试结果。
图10为皮肤皱纹(Primos CR外眼角)测试中外眼角皱纹长度变化率结果。
图11为皮肤皱纹(Primos CR鼻唇沟)测试结果。
图12为皮肤经表皮失水率(脸颊)测试结果。
图13为皮肤血红素含量(脸颊)测试结果。
图14为皮肤光泽度(脸颊)测试结果。
图15为皮肤弹性(脸颊)测试结果。
图16为皮肤紧致(脸颊)测试结果。
图17为斑贴测试结果。
具体实施方式
实施例
实施例1~3提供一种铜盐为葡糖酸铜的双剂
本例提供一种蓝铜肽双剂,包括:铜剂和肽剂,所述肽剂的制备原料按重量百分数,如表1所示,所述铜剂的制备原料按重量百分数计,如表2所示。
表1
表2
本例提供一种双剂的制备方法,包括:将铜剂和肽剂分别混合、均质,得到铜剂和肽剂。
实施例4~8提供一种不同铜盐的双剂
实施例4~8的双剂同实施例1,不同之处在于铜盐不同,如表3所示。
表3
实施例 | 铜盐 |
4 | 孔雀石提取物 |
5 | 氯化铜 |
6 | 叶绿素铜 |
7 | 硫酸铜 |
8 | PCA-Cu |
对照组
本例提供一种蓝铜肽单剂,制备原料按重量百分数,如表4所示。
表4
性能评价
1、功效测试:将实施例1提供的双剂分别加入精华胶囊的分隔的两个腔中,进行测试,如图1~16所示。
2、斑贴测试:将实施例1提供的双剂进行斑贴测试,如图17所示。
3、稳定性:将实施例1~3提供的双剂分别进行稳定性测试,实施例1~3的肽剂满足表5结果,铜剂满足表6结果。
表5
表6
另外,对对照组提供的蓝铜肽单剂进行对比,发现可以看到,当双剂的稳定性都是一些普遍问题,比如原料在高温下稍微变味,在光照下轻微变色,都是正常的活性物变化现象,对产品的功效以及使用并不影响,而将等量的蓝铜肽直接加入一剂精华里的时候,稳定性就比之前的要差很多,在一周左右的时候就产生了不同程度的变色变味。之后产品的稳定性每况愈下,耐寒甚至在最后第八周的时候产生了析出,且其他条件不同的变色以及变味要变得更强烈更重。通过本发明提供的双剂结构可有效提高稳定性,具体如表7所示。
表7
4、铜剂理化性能:将实施例1、实施例4~8提供的铜剂和肽剂按照重量比1:2混合,观察是否出现变色,结果见表8。
表8
由表测试结果可知,本发明提供的双剂具有好的稳定性,可有利于混合后蓝铜肽活性表达,促进敏感肌受损肌肤修复的同时,减少皱纹,提高皮肤的亮度、白度,减少表皮失水,促进肌肤弹性和紧致。
Claims (6)
1.一种蓝铜肽双剂,其特征在于,包括:
肽剂,所述肽剂包括三肽-1;
铜剂,所述铜剂包括铜盐;
肽剂和铜剂分别制备;
所述三肽-1占肽剂的0.2~0.6%;所述铜盐占铜剂的重量百分数为0.9~1%;所述铜盐包括葡糖酸铜、孔雀石提取物、氯化铜、叶绿素铜、硫酸铜、PCA-Cu中的至少一种;所述肽剂的溶剂包括酵母发酵产物滤液和水,所述铜剂的溶剂包括酵母发酵产物滤液和水;所述铜剂还包括吸附剂,所述吸附剂选自环糊精、海藻酸钠、季铵盐中的一种或多种。
2.根据权利要求1所述的蓝铜肽双剂,其特征在于,所述酵母发酵产物滤液选自稻米发酵产物滤液、二裂酵母发酵产物滤液、半乳糖酵母样菌发酵产物滤液、酵母菌发酵产物滤液、裂殖酵母菌丝体发酵产物滤液、乳酸杆菌/豆浆发酵产物滤液、芽孢杆菌/谷氨酸发酵产物滤液中的一种或多种。
3.根据权利要求1所述的蓝铜肽双剂,其特征在于,所述肽剂还包括保湿剂、增稠剂、皮肤调理剂中的一种或多种。
4.根据权利要求1所述的蓝铜肽双剂,其特征在于,所述铜剂还包括保湿剂、增稠剂、皮肤调理剂中的一种或多种。
5.一种根据权利要求1~4任意一项所述的蓝铜肽双剂的使用方法,其特征在于,包括:将铜剂和肽剂混合,发生变色反应。
6.一种根据权利要求1~4任意一项所述的蓝铜肽双剂的应用,其特征在于,用于护肤品。
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