CN115010776B - Extraction method of tartary buckwheat rutin - Google Patents
Extraction method of tartary buckwheat rutin Download PDFInfo
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- CN115010776B CN115010776B CN202210723962.4A CN202210723962A CN115010776B CN 115010776 B CN115010776 B CN 115010776B CN 202210723962 A CN202210723962 A CN 202210723962A CN 115010776 B CN115010776 B CN 115010776B
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- 229960004555 rutoside Drugs 0.000 title claims abstract description 85
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 title claims abstract description 84
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 title claims abstract description 84
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 title claims abstract description 84
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 title claims abstract description 84
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 title claims abstract description 84
- 235000005493 rutin Nutrition 0.000 title claims abstract description 84
- 244000130270 Fagopyrum tataricum Species 0.000 title claims abstract description 67
- 235000014693 Fagopyrum tataricum Nutrition 0.000 title claims abstract description 66
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 238000001816 cooling Methods 0.000 claims abstract description 21
- 238000009210 therapy by ultrasound Methods 0.000 claims abstract description 21
- 238000007710 freezing Methods 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 12
- 238000009835 boiling Methods 0.000 claims abstract description 10
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000009776 industrial production Methods 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 238000002425 crystallisation Methods 0.000 description 8
- 230000008025 crystallization Effects 0.000 description 8
- 238000010812 external standard method Methods 0.000 description 8
- 238000004128 high performance liquid chromatography Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 6
- 239000013078 crystal Substances 0.000 description 5
- 210000000582 semen Anatomy 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 4
- 238000002386 leaching Methods 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 2
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 2
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000005875 quercetin Nutrition 0.000 description 2
- 229960001285 quercetin Drugs 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 206010007191 Capillary fragility Diseases 0.000 description 1
- 241000219051 Fagopyrum Species 0.000 description 1
- 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241000745390 Lophatherum Species 0.000 description 1
- 241000219050 Polygonaceae Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 238000009098 adjuvant therapy Methods 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000000874 microwave-assisted extraction Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005325 percolation Methods 0.000 description 1
- 238000001953 recrystallisation Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/06—Benzopyran radicals
- C07H17/065—Benzo[b]pyrans
- C07H17/07—Benzo[b]pyran-4-ones
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention belongs to the technical field of extraction of active ingredients, and relates to an extraction method of tartary buckwheat rutin; the method comprises the following steps: quick-freezing fresh tartary buckwheat seeds; carrying out ultrasonic treatment on the quick-frozen tartary buckwheat seeds at the temperature ranging from-5 ℃ to-18 ℃; the frequency of ultrasonic vibration is 10KHz-20KHz, and the power is 1-3KW; the ultrasonic vibration time is 10-40s; adding the ultrasonic-treated tartary buckwheat seeds into water, and boiling for 30-60min; cooling the decoction to separate out crude rutin; recrystallizing the crude rutin with hot water to obtain a rutin finished product; the invention does not use organic matters or acid-base solution for extraction, avoids the subsequent treatment steps, reduces the content of other substances in the rutin, effectively improves the purity of the rutin, and is simpler and more convenient in extraction method and beneficial to industrial production.
Description
Technical Field
The invention belongs to the technical field of extraction of active ingredients, and relates to an extraction method of tartary buckwheat rutin.
Background
Tartary buckwheat (F. tartaricum) belongs to the genus Fagopyrum (Polygona ceae) of the family of the Lophatherum (FagopyrumGaerth) as a medicine and food crop, and is recorded in the compendium of Benincase, and has the functions of improving spirit, tonifying qi, benefiting ear eyes, reducing qi, widening intestine and invigorating stomach.
Rutin (rutin) is also called rutin and purple quercetin, is 3-0-rutin of quercetin, has a molecular formula of C27H230O16.3H2O, has a relative molecular weight of 610.51, is yellow crystal powder or amorphous powder, has bitter taste, has a melting point of 177-178 ℃, and can be dissolved in hot water and ethanol. Rutin is a widely available flavonoid. Rutin is used as main component of flavone, can be used as antioxidant and raw material of functional food, has effects of reducing capillary fragility and improving microcirculation, and is clinically used for adjuvant treatment of diabetes, hypertension, etc.
In the prior art, the method for extracting rutin from tartary buckwheat is generally adopted as follows: ethanol reflux extraction, organic solvent percolation, adsorption column chromatography, ultrasonic microwave extraction and the like, but the methods have low raw material utilization rate, low rutin purity and are not suitable for industrial production.
Disclosure of Invention
The invention overcomes the defects of the prior art, and discloses a method for extracting the rutin of tartary buckwheat. So as to improve the utilization rate of the tartary buckwheat and the purity of rutin.
In order to achieve the above purpose, the present invention is realized by the following technical scheme.
The extraction method of the tartary buckwheat rutin is characterized by comprising the following steps of:
1) Quick-freezing the fresh tartary buckwheat seeds.
2) Carrying out ultrasonic treatment on the quick-frozen tartary buckwheat seeds at the temperature ranging from-5 ℃ to-18 ℃; the frequency of ultrasonic vibration is 10KHz-20KHz, and the power is 1-3KW; the time of ultrasonic vibration is 10-40s.
3) Adding the ultrasonic-treated tartary buckwheat seeds into water, and boiling for 30-60min; and then cooling the water decoction to separate out crude rutin.
4) And recrystallizing the crude rutin with hot water to obtain a rutin finished product.
Preferably, the quick-freezing treatment is to make the center temperature of the fresh tartary buckwheat seeds reach-15 ℃ to-18 ℃ within 10 min.
Preferably, the ultrasonic treatment is to embed the vibrator of the ultrasonic device into the tartary buckwheat grains.
Preferably, the vibrator is cooled to-5 ℃ to-10 ℃.
Preferably, the time of the ultrasonic vibration is 30s.
Preferably, in the step 3), the boiled liquid is cooled to 10-20 ℃ and kept stand for 12-24 hours, and crude rutin is separated out.
Preferably, in the step 4), the crude rutin is dissolved by hot water at 98 ℃, filtered while the crude rutin is hot, naturally cooled to room temperature, and left to stand for crystallization, thus obtaining the rutin.
Compared with the prior art, the invention has the following beneficial effects:
According to the invention, fresh tartary buckwheat seeds are frozen in a quick-freezing mode, water in the tartary buckwheat seeds forms fine ice crystals, ultrasonic low-temperature treatment is adopted in the state, and acoustic flow and cavitation effect are generated in the tartary buckwheat seeds, so that the ice crystals form uniform and fine crystal grains, and the tartary buckwheat rutin is more easily released from the constraint of macromolecular substances under the action of the crystal grains; then through the steps of boiling water extraction and recrystallization, rutin components can be effectively extracted.
The invention does not use organic matters or acid-base solution for extraction, avoids the subsequent treatment steps, reduces the content of other substances in the rutin, effectively improves the purity of the rutin, and is simpler and more convenient in extraction method and beneficial to industrial production.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the invention is further described in detail by combining the embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention. The following describes the technical scheme of the present invention in detail with reference to examples, but the scope of protection is not limited thereto.
Example 1
A method for extracting rutin from Fagopyrum tataricum comprises the following specific steps:
1) 100g of fresh tartary buckwheat seeds which are newly harvested and full are cleaned and dried, and then quick-freezing treatment is carried out on the fresh tartary buckwheat seeds: the quick-freezing treatment is to make the center temperature of the fresh tartary buckwheat seeds reach-15 ℃ to-18 ℃ within 10 min.
2) Then carrying out ultrasonic treatment on the quick-frozen tartary buckwheat seeds at the temperature of-5 ℃; the frequency of ultrasonic vibration is 10KHz, and the power is 3KW; the time of ultrasonic vibration was 20s. The ultrasonic treatment is specifically carried out by cooling vibrator of ultrasonic device to-5deg.C, and then burying into fresh semen Fagopyri Tatarici.
3) Directly adding the fresh tartary buckwheat seeds subjected to ultrasonic treatment into cold water, and boiling for 30min; and then cooling the water decoction to 10 ℃ and standing for 24 hours to separate out crude rutin.
4) Dissolving crude rutin with 98deg.C hot water, filtering while hot, naturally cooling to room temperature, standing for crystallization to obtain rutin.
The high performance liquid chromatography external standard method is adopted to detect, and the mass content of rutin in the rutin refined product obtained by the embodiment is 96.9%.
Example 2
A method for extracting rutin from Fagopyrum tataricum comprises the following specific steps:
1) 100g of fresh tartary buckwheat seeds which are newly harvested and full are cleaned and dried, and then quick-freezing treatment is carried out on the fresh tartary buckwheat seeds: the quick-freezing treatment is to make the center temperature of the fresh tartary buckwheat seeds reach-15 ℃ to-18 ℃ within 10 min.
2) Then carrying out ultrasonic treatment on the quick-frozen tartary buckwheat seeds at the temperature of-18 ℃; the frequency of ultrasonic vibration is 20KHz, and the power is 2KW; the time of ultrasonic vibration was 30s. The ultrasonic treatment is specifically carried out by cooling vibrator of ultrasonic device to-10deg.C, and then burying into fresh semen Fagopyri Tatarici.
3) Directly adding the fresh tartary buckwheat seeds subjected to ultrasonic treatment into cold water, and boiling for 60 minutes; and then cooling the water decoction to 20 ℃ and standing for 24 hours to separate out crude rutin.
4) Dissolving crude rutin with 98deg.C hot water, filtering while hot, naturally cooling to room temperature, standing for crystallization to obtain rutin.
The high performance liquid chromatography external standard method is adopted to detect, and the mass content of rutin in the rutin refined product obtained by the embodiment is 98.7%.
Example 3
A method for extracting rutin from Fagopyrum tataricum comprises the following specific steps:
1) 100g of fresh tartary buckwheat seeds which are newly harvested and full are cleaned and dried, and then quick-freezing treatment is carried out on the fresh tartary buckwheat seeds: the quick-freezing treatment is to make the center temperature of the fresh tartary buckwheat seeds reach-18 ℃ within 10 min.
2) Then carrying out ultrasonic treatment on the quick-frozen tartary buckwheat seeds at the temperature of-10 ℃; the frequency of ultrasonic vibration is 20KHz, and the power is 1KW; the time of ultrasonic vibration was 40s. The ultrasonic treatment is specifically carried out by cooling vibrator of ultrasonic device to-10deg.C, and then burying into fresh semen Fagopyri Tatarici.
3) Directly adding the fresh tartary buckwheat seeds subjected to ultrasonic treatment into cold water, and boiling for 40min; and then cooling the water decoction to 20 ℃ and standing for 24 hours to separate out crude rutin.
4) Dissolving crude rutin with 98deg.C hot water, filtering while hot, naturally cooling to room temperature, standing for crystallization to obtain rutin.
The high performance liquid chromatography external standard method is adopted to detect, and the mass content of rutin in the rutin refined product obtained by the embodiment is 97.1%.
Example 4
A method for extracting rutin from Fagopyrum tataricum comprises the following specific steps:
1) 100g of fresh tartary buckwheat seeds which are newly harvested and full are cleaned and dried, and then quick-freezing treatment is carried out on the fresh tartary buckwheat seeds: the quick-freezing treatment is to make the center temperature of the fresh tartary buckwheat seeds reach-15 ℃ within 10 min.
2) Then carrying out ultrasonic treatment on the quick-frozen tartary buckwheat grains under the environment condition of-10 ℃; the frequency of ultrasonic vibration is 10KHz, and the power is 3KW; the time of ultrasonic vibration was 30s. The ultrasonic treatment is specifically carried out by cooling vibrator of ultrasonic device to-10deg.C, and then burying into fresh semen Fagopyri Tatarici.
3) Directly adding the fresh tartary buckwheat seeds subjected to ultrasonic treatment into cold water, and boiling for 40min; and then cooling the water decoction to 20 ℃ and standing for 24 hours to separate out crude rutin.
4) Dissolving crude rutin with 98deg.C hot water, filtering while hot, naturally cooling to room temperature, standing for crystallization to obtain rutin.
The high performance liquid chromatography external standard method is adopted to detect, and the mass content of rutin in the rutin refined product obtained by the embodiment is 98.2%.
Example 5
A method for extracting rutin from Fagopyrum tataricum comprises the following specific steps:
1) 100g of fresh tartary buckwheat seeds which are newly harvested and full are cleaned and dried, and then quick-freezing treatment is carried out on the fresh tartary buckwheat seeds: the quick-freezing treatment is to make the center temperature of the fresh tartary buckwheat seeds reach-15 ℃ within 10 min.
2) Then carrying out ultrasonic treatment on the quick-frozen tartary buckwheat grains under the environment condition of-18 ℃; the frequency of ultrasonic vibration is 10KHz, and the power is 2KW; the time of ultrasonic vibration was 40s. The ultrasonic treatment is specifically carried out by cooling vibrator of ultrasonic device to-10deg.C, and then burying into fresh semen Fagopyri Tatarici.
3) Directly adding the fresh tartary buckwheat seeds subjected to ultrasonic treatment into cold water, and boiling for 60 minutes; and then cooling the water decoction to 10 ℃ and standing for 24 hours to separate out crude rutin.
4) Dissolving crude rutin with 98deg.C hot water, filtering while hot, naturally cooling to room temperature, standing for crystallization to obtain rutin.
The high performance liquid chromatography external standard method is adopted to detect, and the mass content of rutin in the rutin refined product obtained by the embodiment is 97.6%.
Comparative example 1
100G of complete fresh tartary buckwheat seeds are selected and cleaned, then the cleaned tartary buckwheat seeds are put into clear water and soaked for 2-24 hours at the temperature of 10-50 ℃, the tartary buckwheat seeds are drained after the soaking is finished, then the drained tartary buckwheat seeds are dried in the environment with the temperature of 105 ℃, lime water with the pH value of 8-9 de is added, the leaching is carried out for different times under the condition of ultrasonic constant temperature leaching at the temperature of 80 ℃, and the leaching yield of rutin is measured.
The quality content of rutin in the rutin refined product obtained in comparative example 1 is 85.1% by detection with an external standard method of high performance liquid chromatography.
Comparative example 2
Crushing 100g of tartary buckwheat seeds to a particle size of 2mm, adding 2000g of calcium hydroxide aqueous solution, stirring and extracting for 5 hours, filtering, adjusting the pH value of the filtrate to 6.0 by using hydrochloric acid solution with the mass concentration of 5%, and then ultrafiltering to obtain ultrafiltrate; eluting the obtained ultrafiltration membrane filtrate with a chromatographic column, collecting eluent, concentrating and drying to obtain crude rutin, dissolving the crude rutin with 75g of hot water at 98 ℃, filtering while the crude rutin is hot, naturally cooling to room temperature, standing for crystallization for 12h, suction filtering, and drying to obtain rutin.
The quality content of rutin in the refined rutin product obtained in comparative example 2 is 89.2% by detection with an external standard method of high performance liquid chromatography.
Comparative example 3
Soaking 100g of fresh tartary buckwheat seeds in 50 ℃ water for 24 hours, and boiling for 40 minutes; and then cooling the water decoction to 20 ℃ and standing for 24 hours to separate out crude rutin. Dissolving crude rutin with 98deg.C hot water, filtering while hot, naturally cooling to room temperature, standing for crystallization to obtain rutin.
The high performance liquid chromatography external standard method is adopted to detect, and the mass content of rutin in the rutin refined product obtained in the comparative example 3 is 70.5%.
While the invention has been described in detail in connection with specific preferred embodiments thereof, it is not to be construed as limited thereto, but rather as a result of a simple deduction or substitution by a person having ordinary skill in the art to which the invention pertains without departing from the scope of the invention defined by the appended claims.
Claims (4)
1. The extraction method of the tartary buckwheat rutin is characterized by comprising the following steps of:
1) Quick-freezing fresh tartary buckwheat seeds; the quick-freezing treatment is to make the center temperature of the fresh tartary buckwheat seeds reach-15 ℃ to-18 ℃ within 10 min;
2) Carrying out ultrasonic treatment on the quick-frozen fresh tartary buckwheat seeds at the temperature ranging from-5 ℃ to-18 ℃; the ultrasonic treatment is that firstly, the vibrator is cooled to the temperature of between-5 ℃ and-10 ℃, and then the vibrator of the ultrasonic device is buried in fresh tartary buckwheat grains; the frequency of ultrasonic vibration is 10KHz-20KHz, and the power is 1-3KW; the ultrasonic vibration time is 10-40s;
3) Adding the fresh tartary buckwheat seeds subjected to ultrasonic treatment into water, and boiling for 30-60min; cooling the decoction to separate out crude rutin;
4) And recrystallizing the crude rutin with hot water to obtain a rutin finished product.
2. The method for extracting rutin from Fagopyrum tataricum of claim 1, wherein the time of ultrasonic vibration is 30s.
3. The method for extracting rutin from Fagopyrum tataricum according to claim 1, wherein in step 3), the decoction is cooled to 10-20deg.C and kept stand for 12-24h to precipitate crude rutin.
4. The method for extracting rutin from Fagopyrum tataricum according to claim 1, wherein in step 4), the crude rutin is dissolved in 98 ℃ hot water, filtered while hot, naturally cooled to room temperature, and left to crystallize to obtain rutin.
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|---|---|---|---|---|
| KR20060056583A (en) * | 2004-11-22 | 2006-05-25 | 춘천시 | A method for preparing of rutin extract and rutin powder from buckwheat herbs |
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| CN102002083A (en) * | 2010-12-20 | 2011-04-06 | 河南智晶生物科技有限公司 | Method for extracting high-purity rutin with flash-extraction technology |
| CN103864868A (en) * | 2014-03-01 | 2014-06-18 | 张家港威胜生物医药有限公司 | Green extraction process of natural active flavone-rutin |
| CN104664236A (en) * | 2015-03-20 | 2015-06-03 | 河北科技大学 | Method for promoting enrichment of rutin of buckwheat grains |
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