CN104664236A - Method for promoting enrichment of rutin of buckwheat grains - Google Patents
Method for promoting enrichment of rutin of buckwheat grains Download PDFInfo
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- CN104664236A CN104664236A CN201510124314.7A CN201510124314A CN104664236A CN 104664236 A CN104664236 A CN 104664236A CN 201510124314 A CN201510124314 A CN 201510124314A CN 104664236 A CN104664236 A CN 104664236A
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Abstract
The invention relates to a treatment method of crops and particularly relates to a method for promoting enrichment of rutin of buckwheat grains. The method comprises the following steps: hulling buckwheat grains; freezing and melting repeatedly; soaking in acidic electrolysis water and drying to obtain rutin-enriched buckwheat grains. The method for promoting enrichment of rutin of buckwheat grains, disclosed by the invention, creates an adverse condition by adopting a repeated freezing and melting method and a micro-acidic electrolysis water soaking method; by adopting a coercing method, a phenylpropane type secondary metabolism product in the buckwheat grains is changed, so that rutin accumulation of a buckwheat raw material can be remarkably improved, and therefore the functionality of a final buckwheat relevant product can be enhanced.
Description
Technical field
The present invention relates to a kind of crops processing method, be specially the method promoting the enrichment of buckwheat seed rutin.
Background technology
Buckwheat, it is the important rain fed crops of China, not only be rich in protein, carbohydrate, amino acid, unrighted acid and vitamin, be especially rich in the unexistent chlorophyll of other cereal crops and flavone compound, some flavones in buckwheat are determined by people, as rutin, Quercetin, quercetin, kaempferia galamga, Polygonum/Lutonaretin and Vitexin/Saponaretin.In these flavone compounds, rutin (citrin) is the main and most important flavonoid components of buckwheat, and does not contain at other cereal.
Rutin, also known as rutin sophorin, is the main component of citrin, belongs to flavone derivative, and content comparatively horn of plenty in bitter buckwheat is the main source of meals flavones.Especially, in the Flavonoid substances contained by bitter buckwheat, rutin accounts for more than 80%.Research proves, rutin has very strong non-oxidizability, can reduce human body blood fat and cholesterol, maintains microcirculation, improves immunity of organisms, has adjuvant treatment efficacy for diabetes, hypertension etc.In addition rutin also has the effectiveness of certain anticancer, sterilization and anti-inflammatory.
Current " problem of increasing income " for plant functional components mainly can be started with from two aspects: one is made better products by breeding technique, cultivates the Dominant variety of high yield object functional component; Two is the metabolic pathways from Plant functional composition, adopts the means such as physics, chemistry to intervene plant metabolism, carries out the enrichment of object functional components with this.
Make better products mainly through breeding technique for rutin enrichment in buckwheat seed at present, cultivate and plant the Dominant variety of high yield object functional component rutin, for the later stage, the process of buckwheat seed yet there are no relevant report.
Summary of the invention
For above-mentioned technical problem, the invention provides the method for a kind of later stage to the process of buckwheat seed, object is the rutin accumulation improving buckwheat, and concrete technical scheme is:
Promote the method for buckwheat seed rutin enrichment, comprise the following steps:
(1) select normal, shell without the buckwheat seed of disease and pest, the buckwheat seed after shelling carry out freezing, melt process repeatedly 1-3 time, freezing processing condition is-40 ~-80 DEG C, and freezing 6-36 hour, melts 2-24 hour, and the condition of melting is left at room temperature;
(2) use acidic electrolysis water soaking, the pH 2.0 ~ 6.5 of acidic electrolytic water, effective chlorine density 10 ~ 100mg/l, soak 2-8 hour;
(3) the buckwheat seed soaked carries out drying process, namely obtains the buckwheat seed of rutin enrichment.
Wherein, acidic electrolytic water preparation method is, acidic electrolytic water, and employing electrolyte is the sodium chloride solution of mass concentration 1%, and electrolytic water generating device carries out electrolysis, and decomposition voltage is 24V, and Faradaic current is 0.1A.Gained subacidity brine electrolysis physical and chemical index is pH is 2.0 to 6.5, and effective chlorine density is 10 ~ 100mg/l.
The method of promotion buckwheat seed rutin provided by the invention enrichment, adverse environmental factor is created with multigelation and subacidity brine electrolysis immersion process, by the method for coercing, phenylpropyl alcohol alkanes secondary metabolites in buckwheat seed changes, the rutin accumulation of buckwheat can be significantly improved, the functional of final buckwheat Related product can be strengthened thus.
Detailed description of the invention
The specific embodiment of the present invention is described in conjunction with the embodiments.
Embodiment 1
Promote the method for buckwheat seed rutin enrichment, comprise the following steps:
(1) selection normally, without after the buckwheat seed shelling of disease and pest, multigelation process, multigelation technical parameter is selected: cryogenic freezing after 24 hours, melts 5 hours to-80 DEG C of maintenances, then is refrigerated to-80 DEG C of maintenances 12 hours.
(2) immersion treatment is carried out 4 hours with the acidic electrolytic water of pH 5.0, effective chlorine density 30mg/l.
(3) the buckwheat seed removing excessive moisture after process, 30 DEG C of oven dry, obtain the buckwheat seed of rutin enrichment.
Embodiment 2
Promote the method for buckwheat seed rutin enrichment, comprise the following steps:
(1) selection normally, without after the buckwheat seed shelling of disease and pest, multigelation process 3 times, multigelation technical parameter is selected: cryogenic freezing after 36 hours, melts 2 hours to-40 DEG C of maintenances, then is refrigerated to-40 DEG C of maintenances 12 hours.
(2) immersion treatment is carried out 2 hours with the acidic electrolytic water of pH2.0, effective chlorine density 100mg/l.
(3) the buckwheat seed removing excessive moisture after process, 30 DEG C of oven dry, obtain the buckwheat seed of rutin enrichment.
Buckwheat seed Assaying of Rutin:
Get the buckwheat seed rutin that untreated buckwheat seed rutin and the present embodiment 1 processed, take each processed group 1g sample and put into mortar, add 60% ethanol fully to grind, pour into after grinding in test tube, put into 80 DEG C of thermostat water bath lixiviate 2h, every 15min concussion once, 8000r/min, centrifugal 15min.Finally get supernatant and remove precipitation, supernatant constant volume is to 5mL.Rutin content adopts high performance liquid chromatography (HPLC) to measure, and actual conditions is: C18 chromatographic column, and methyl alcohol-0.4% phosphate aqueous solution (55:45) is mobile phase, and determined wavelength is 257nm.Result represents with mg/100g.Result is as shown in table 1.
Table 1 buckwheat seed rutin content test result
| Sample | Rutin content (mg/100g) |
| Untreated buckwheat seed rutin | 320.48 |
| The buckwheat seed rutin of the present embodiment process | 608.44 |
As can be seen from the results, the pre-treatment that buckwheat seed is carried out in multigelation skill provided by the invention and acidic electrolytic water coupling can significantly improve the rutin content of buckwheat seed.Purposes of the present invention has good industrial applicibility, simple to operate, and security is good.
Claims (2)
1. promote the method for buckwheat seed rutin enrichment, it is characterized in that, comprise the following steps:
(1) garbled buckwheat seed shelling, the buckwheat seed after shelling carry out freezing, melt and repeatedly process 1-3 time, freezing processing condition is-40 ~-80 DEG C, and freezing 6-36 hour, melts 2-24 hour, and the condition of melting is left at room temperature;
(2) use acidic electrolysis water soaking, the pH 2.0 ~ 6.5 of acidic electrolytic water, effective chlorine density 10 ~ 100mg/l, soak 2-8 hour;
(3) the buckwheat seed soaked carries out drying process, namely obtains the buckwheat seed of rutin enrichment.
2. the method for promotion buckwheat seed rutin according to claim 1 enrichment, is characterized in that, described acidic electrolytic water, employing electrolyte is the sodium chloride solution of mass concentration 1%, electrolytic water generating device carries out electrolysis, and decomposition voltage is 24V, and Faradaic current is 0.1A.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510124314.7A CN104664236B (en) | 2015-03-20 | 2015-03-20 | Promote the method for buckwheat seed rutin enrichment |
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| CN201510124314.7A CN104664236B (en) | 2015-03-20 | 2015-03-20 | Promote the method for buckwheat seed rutin enrichment |
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| CN104664236A true CN104664236A (en) | 2015-06-03 |
| CN104664236B CN104664236B (en) | 2017-12-15 |
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Cited By (3)
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| CN110622808A (en) * | 2019-09-26 | 2019-12-31 | 中国科学院昆明植物研究所 | Planting method for promoting growth of bletilla striata and accumulation of effective components |
| CN115010776A (en) * | 2022-06-24 | 2022-09-06 | 山西龙荞生物科技有限公司 | Method for extracting buckwheat rutin |
| NL2035033B1 (en) * | 2023-06-08 | 2024-01-26 | Shanxi Institute For Functional Food Shanxi Agricultural Univ | Method for inhibiting bitter taste of tartary buckwheat seeds and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115010776A (en) * | 2022-06-24 | 2022-09-06 | 山西龙荞生物科技有限公司 | Method for extracting buckwheat rutin |
| CN115010776B (en) * | 2022-06-24 | 2024-04-19 | 山西助潞腾飞数智科技有限公司 | Extraction method of tartary buckwheat rutin |
| NL2035033B1 (en) * | 2023-06-08 | 2024-01-26 | Shanxi Institute For Functional Food Shanxi Agricultural Univ | Method for inhibiting bitter taste of tartary buckwheat seeds and its application |
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