CN114982588B - Shale soil disease-inhibiting matrix and preparation method and application thereof - Google Patents
Shale soil disease-inhibiting matrix and preparation method and application thereof Download PDFInfo
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- CN114982588B CN114982588B CN202210345103.6A CN202210345103A CN114982588B CN 114982588 B CN114982588 B CN 114982588B CN 202210345103 A CN202210345103 A CN 202210345103A CN 114982588 B CN114982588 B CN 114982588B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a shale soil disease-suppressing matrix, a preparation method and application thereof, wherein the shale soil disease-suppressing matrix comprises the following components in percentage by mass: the shale soil disease-inhibiting matrix is prepared by mixing and fermenting 40-60% of shale soil, 5-15% of vermiculite, 10-20% of rice bran, 2.5-7.5% of humic acid, 1.5-3.5% of industrial brown sugar and 5-10% of microorganism composite bacteria, and the obtained shale soil disease-inhibiting matrix generates three kinds of microorganism beneficial bacteria groups comprising bacteria, fungi, actinomycetes and the like in soil, forms a biological protection barrier for plant rhizosphere, is beneficial to stably preventing and controlling the occurrence of vegetable soil-borne diseases in facility fields, and reduces the problems of formation of continuous cropping obstacles of facility vegetables, plant diseases and insect pests and the like.
Description
Technical Field
The invention relates to the field of agricultural planting and biological control, in particular to a shale soil disease-inhibiting matrix for preventing and controlling facility soil-borne diseases, and a preparation method and application thereof.
Background
Vegetables are food indispensable to human society. With the development of specialization and intensification of vegetables, the problems of single variety, high frequency of multiple cropping indexes, large input amount of chemical fertilizers, pesticides and the like and continuous cropping obstacles of vegetables such as melons, solanaceae and the like are increasingly serious. Investigation has shown that the main cause of continuous cropping obstacles in greenhouse vegetables is due to soil-borne diseases. After continuous cropping, the rhizosphere microbial community structure can be obviously changed, so that the quantity of pathogenic bacteria, fungi, nematodes and the like is greatly increased, the microbial area structure and the microbial diversity are destroyed, the growth and development of crops are blocked, even the life is endangered, and the yield and the economic value of the crops are greatly reduced.
Rhizosphere soil microbiota is the first line of defense against invasion of pathogenic bacteria into plant roots. The microbial community inoculated with beneficial microorganisms can form a biological barrier around the rhizosphere of crops, inhibit harmful microorganisms and recruit functional microorganisms, promote the immunity of crops and improve the disease resistance of the crops. With increasing importance of food safety, sustainable control of plant diseases and insect pests is required, and biological control is an effective technical measure for preventing and controlling soil-borne diseases and eliminating continuous cropping obstacles, and has been widely applied to the production of facility vegetables.
However, the current research is mainly focused on the interaction relationship among plants, pathogenic bacteria and biocontrol bacteria, the effect of microbiome/group on plant rhizosphere is ignored, and the stable disease suppression in the field cannot be realized due to the weak rhizosphere colonization capability of single beneficial bacteria.
Disclosure of Invention
The invention provides a shale soil disease-inhibiting matrix for preventing and controlling facility soil-borne diseases, a preparation method and application thereof.
In a first aspect of the embodiment of the invention, a shale soil disease suppressing matrix is provided, wherein the shale soil disease suppressing matrix comprises the following components in percentage by mass: 40 to 60 percent of shale soil, 5 to 15 percent of vermiculite, 10 to 20 percent of rice bran, 2.5 to 7.5 percent of humic acid, 1.5 to 3.5 percent of industrial brown sugar and 5 to 10 percent of microorganism composite bacteria are mixed and fermented to prepare the fertilizer;
wherein the microorganism complex bacteria comprise ferment bacteria propagation strains, 10.0x10 8 cfu/g lilyturf-pt 361 spore liquid, 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid.
The invention submits the lilyturf-sp pt361 spore liquid to the approved institution for preservation, and the preservation number is as follows: cctccc NO: m2016681; the classification is named: lilyturf pt361; the preservation date is 2016, 11 and 25; the preservation units are: china center for type culture Collection; preservation address: in the Wuhan university of No. 299 of Wuhan district of Wuhan, hubei province, the first appendage is opposite, china center for type culture collection.
The invention submits Bacillus bailii NH-1 bacterial liquid to the approved institution for patent for preservation, and the preservation number is: cctccc NO: m2017336; the classification is named: bacillus bailii NH-1; the preservation date is 14 days of 2017, 6 months; the preservation units are: china center for type culture Collection; preservation address: in the Wuhan university of No. 299 of Wuhan district of Wuhan, hubei province, the first appendage is opposite, china center for type culture collection.
Further, the shale soil disease-suppressing matrix comprises the following components in percentage by mass: 60% shale soil, 10% vermiculite, 15% rice bran, 5% humic acid, 2.5% industrial brown sugar and 7.5% microorganism composite bacteria.
Further, the microorganism composite bacteria comprise the following components in percentage by mass: 3 to 7 percent of ferment bacteria propagation strain, 0.25 to 0.75 percent of 10.0x10 8 cfu/g of lilyturf-pt 361 spore liquid, 1-3% of 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid.
Further, the microorganism composite bacteria comprise the following components in percentage by mass: 5% of ferment bacteria propagation strain, 0.5% of 10.0X10 8 cfu/g lilyturf-pt 361 spore liquid, 2% 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid.
In a second aspect of the embodiments of the present invention, there is provided a method for preparing a shale soil disease suppressing matrix, wherein the method comprises:
firstly, weighing shale soil, vermiculite, rice bran, humic acid, industrial brown sugar and microorganism composite bacteria according to mass percentage;
step two, uniformly mixing the microorganism complex bacteria and rice bran, spraying the prepared brown sugar water onto the microorganism complex bacteria and rice bran mixture, stirring while spraying, and adjusting the water content to be 45-55%;
uniformly scattering the mixture obtained in the step two, humic acid and vermiculite on shale soil, and stirring while scattering, so as to keep the water content at 45-55%;
piling the mixture prepared in the step three on a clean panel, sieving, piling up a mountain shape with the height of about 1.2m, and covering for heat preservation;
turning the pile: after 48 hours from the date of manufacture, turning up and down and piling up into a mountain shape, and turning up 1 time every day;
step six, drying: spreading on the clean panel for natural drying or warm air drying;
and step seven, checking, sieving and packaging.
And in the fifth step, turning the pile at the temperature of 50-55 ℃.
Further, turning the stack 6 times in the fifth step.
Further, the water content of the mixture after air drying in the step six is 5-10%.
In a third aspect of the embodiments of the present invention, there is provided the use of shale soil disease suppressing matrix in the construction of a disease suppressing rhizosphere soil micro-ecological environment.
Further, methods of use of the shale soil disease suppression matrix include, but are not limited to, hole application, strip application, broadcast application.
Further, the shale soil disease-suppressing matrix generates beneficial microbial flora of three types of bacteria, fungi and actinomycetes in soil.
Compared with the prior art, the invention has the beneficial effects that: the invention provides a shale soil disease-inhibiting matrix and a preparation method and application thereof, wherein a composite microorganism group with specific functions is cultivated by adding microorganism composite bacteria consisting of ferment bacteria, lilyturf and bacillus bailii on the shale soil matrix through fermentation, namely, the shale soil disease-inhibiting matrix generates microorganism beneficial flora covering three categories of bacteria, fungi and actinomycetes in soil to form a biological protection barrier for plant rhizosphere, thus constructing a disease-inhibiting rhizosphere soil microecological environment, having strong colonization capability compared with single beneficial bacteria, and being beneficial to stably preventing and controlling vegetable soil-borne diseases in facility fields; the plant growth can be stimulated, the soil hardening is relieved, the soil ecological environment is improved, and the like, so that the problems of formation of continuous cropping obstacles of the facility vegetables, plant diseases and insect pests and the like are effectively solved; in addition, the application of chemical pesticides is reduced, and a novel technology and a novel path for improving the soil health are provided.
Drawings
FIG. 1 is a flow chart of the preparation of shale soil disease-suppressing matrix
FIG. 2 is a diagram showing high throughput sequencing analysis of shale soil-borne disease-suppressing matrix bacterial populations in accordance with the present invention
FIG. 3 is a high throughput sequencing analysis chart of shale soil-borne disease matrix fungus populations provided by the invention
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is shown, however, only some, but not all embodiments of the invention are shown. All other embodiments, which can be made by one of ordinary skill in the art without creative efforts, are within the scope of the present invention based on the embodiments of the present invention.
The test methods, in which specific conditions are not noted in the following examples, are generally conducted under conventional conditions. The reagents or apparatus are not designated the manufacturer and are conventional products commercially available.
Examples
The embodiment provides a shale soil disease-suppressing matrix, wherein the shale soil disease-suppressing matrix comprises the following components in percentage by mass: 60% shale soil, 60% vermiculite, 15% rice bran, 5% humic acid, 2.5% industrial brown sugar, 5% ferment bacteria propagation strain, 0.5% 10.0X10% 8 cfu/g lilyturf-pt 361 spore liquid, 2% 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid.
The embodiment selects ferment bacteria, lilyturf lilacinus and bacillus bailii to perform functional microorganism tissue culture, so as to construct the disease-inhibiting rhizosphere soil micro-ecological environment. The ferment bacteria are also developed by Japanese island, can generate fermentation products such as multiple vitamins, nucleic acid, mycoprotein and the like in the process of decomposition fermentation, has quite rich nutritive value, and can recruit beneficial microorganisms for propagation and growth; the lilyturf turber pt361 (CCTCC NO: M2016681) can generate insecticidal and bacteriostatic active substances, has good biological control effects on various nematodes such as root-knot nematodes, white fly, red spiders, leaf cutting ants, bakanae disease of rice, tomato root rot, wheat scab, corn small spot, wheat leaf spot and the like, has various functions such as phosphate dissolving, growth promoting and the like, has stronger capability of resisting external environmental pressure, can resist injuries caused by drying, heat and ultraviolet radiation in living environment, and maintains self capability unaffected; bacillus belicus NH-1 (CCTCC NO: M2017336) has the functions of preventing and inhibiting diseases, enhancing plant disease resistance, improving the absorption of soil microbiota and crops to soil nutrients, generating heat-resistant and stress-resistant spores, facilitating the production of biocontrol bactericides, processing dosage forms and survival, colonization and reproduction in the environment, and has reasonable collocation of microorganism types, strong functionality, effective degradation of chemical residues, improvement of soil ecological environment, bacteriostasis, disease prevention and soil improvement, and obvious effect of relieving continuous cropping obstacle of facility vegetables.
The shale soil is adopted as a carrier for fixing microorganisms, is a natural iron-rich magnesium aluminum silicate clay mineral with excellent adsorption performance and colloid performance, is a friendly carrier for carrying microorganisms, can adsorb nutrient elements in soil, increases the granular structure of the soil, maintains and adjusts the fertility of the soil, adjusts the pH value of the soil and promotes the growth of plants; vermiculite can improve the water permeability and air permeability of soil to a certain extent; humic acid, rice bran and brown sugar can also provide a C source for plant growth, have complexing and buffering effects, and can improve soil fertility and physical and chemical properties of soil.
The preparation method of the shale soil disease-suppressing matrix provided by the embodiment is shown in fig. 1, and comprises the following steps:
step one, setting the total mass to be 50kg, and sequentially weighing 30kg of shale soil, 5kg of vermiculite, 7.5kg of rice bran, 2.5kg of humic acid, 1.25kg of industrial brown sugar, 2.5kg of ferment strain propagation strain and 0.25kg of 10.0X10 according to the mass percentage 8 cfu/g of lilyturf-pt 361 spore liquid, 1kg of 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid;
pouring a proper amount of clear water or warm water into a container filled with 1.25kg of industrial brown sugar, and dissolving into brown sugar water;
step three, 2.5kg of ferment bacteria are used for propagating strains, 0.25kg of 10.0X10 8 cfu/g of lilyturf-pt 361 spore liquid, 1kg of 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid and 7.5kg rice bran are uniformly mixed, prepared red sugar water is sprayed on a mixture of microorganism complex bacteria and rice bran, and the water content is regulated to be 45-55% while spraying and stirring;
uniformly scattering the mixture obtained in the step three, 2.5kg of humic acid and 5kg of vermiculite on 30kg of shale soil, stirring while scattering, and adjusting the water content to be 45-55%;
piling the mixture prepared in the step four on a clean panel (cement floor or floor), sieving, piling up a mountain shape with the height of about 1.2m, and covering with a clean mat for heat preservation;
turning the pile: after 48 hours from the date of manufacture, when the temperature of the mixture in the step five is within the range of 50-55 ℃, turning the mixture up and down and piling up the mixture into a mountain shape, covering the mountain shape with a clean mat, and preserving heat;
turning 1 time of the mixture at 50-55 ℃ every day, continuously turning 5 times, fully growing microbial strains after 6 times of turning, forming microbial communities, stabilizing colony morphology, and reducing the temperature of the microbial communities to normal temperature, wherein the colony morphology is unchanged;
step seven, drying: spreading the mixture in the fifth step on a clean panel for natural drying or warm air drying, wherein the mixture cannot be irradiated and air-dried under strong sunlight, and the water content of the air-dried mixture is between 5 and 10 percent;
and step eight, checking whether the air-dried mixture in the step seven meets the requirement of preservation humidity standard, sieving the very compound by using a sieve to remove large lumps, and finally filling the mixture into bags for subpackaging and preservation or directly applying the mixture to protected facilities.
The shale soil disease-suppressing matrix provided by the embodiment is applied to construction of disease-suppressing rhizosphere soil micro-ecological environment. After the shale soil disease-inhibiting matrix is used, three kinds of beneficial microbial flora covering bacteria, fungi, actinomycetes and the like can be formed in soil, the beneficial microbial flora acts on plant rhizosphere, the colonization capacity is strong, a biological barrier can be formed around the plant rhizosphere, harmful microorganisms are inhibited, the immune function of plants can be promoted, and the occurrence probability of soil-borne diseases is greatly reduced.
Application example 1
Microbial flora and species detection
1. Test site and method
The experimental place is a modern agricultural research and development base in Henan. And (3) taking a fermented shale soil disease-inhibiting matrix sample (the storage time is 1 month, 3 months and 5 months respectively), and extracting soil DNA (deoxyribonucleic acid) for measuring the variety diversity of microbial flora.
2. Test results
The high-throughput sequencing of shale soil disease-suppressing matrix microbial flora is as follows: among the bacteria are various beneficial bacterial groups such as Bacillus bailii (Bacillus velezensis), lactobacillus delbrueckii (Lactobacillus pontis), lactobacillus acidophilus (Weissella cibarla), lactobacillus acidophilus (Lactobacillus acidiplscls), corynebacterium actinomycetes (Corynebacterium), streptomyces (Streptomyces) and Myxomycetes (Carnimonas), as shown in FIG. 2; the fungi are mainly shown in FIG. 3 by various yeasts (Saccharomyces) and purple spore bacteria (Purpureocillium lilacinum). The result shows that the microbial flora of the disease-inhibiting matrix prepared by the method covers three kinds of beneficial flora such as bacteria, fungi and actinomycetes, the microbial flora in the soil after the shale soil disease-inhibiting matrix acts is rich in type and structure, and the microbial flora has stable performance, and compared with a single microbial strain, the microbial flora forms a biological barrier around the plant rhizosphere, so that soil-borne diseases are reduced.
Application example 2
Shale soil disease-suppressing matrix field root-knot nematode prevention and control test
1. Test site and method
The experimental site is carried out in a facility protection place where cucumber root-knot nematodes are seriously generated in Henan modern agriculture research and development base. The shale soil disease-suppressing matrix sets 6 treatments for the district control effect test of cucumber root-knot nematodes: 10% fosthiazate (3 g/hole), 0.5% abamectin emulsifiable concentrate (3 mL/hole), disease-suppressing matrix (50 g/hole), disease-suppressing matrix (100 g/hole), disease-suppressing matrix (200 g/hole) and blank control, wherein each treatment is provided with 3 cells, and the total random design is carried out, and the area of each cell is 8.4m 2 (5.6mX1.5 m), 60 cucumber plants were transplanted in total. When cucumber transplanting, the shale soil disease-suppressing matrix or medicament is applied into the holes by a hole application method, then robust aseptic cucumber seedlings are transplanted, and all the cells adopt the same management conditions. Cucumber transplantingAnd (5) detecting the population density of the root knot nematodes J2 in the soil of each district after 0, 11, 35 and 65d, obtaining the dynamic changes of the population of the J2 in the soil at different periods, and calculating the J2 proliferation index (RI) =the population density of the J2 in the soil (Pf)/the population density of the initial soil J2 (Pi) after 65d transplanting. After cucumber transplanting, 11, 35 and 65 days, 5 cucumber plants are dug randomly in each district, root knots of all treated root system samples are classified by referring to Bridge and Page methods, and disease indexes and prevention effects are calculated. Root knot nematode grading standard: level 0: the root system has no root knot; stage 1: root knots are arranged on less than 10 percent of root systems, but the root knots are not connected with each other; 2 stages: root knots are arranged on 11% -30% of root systems, and only a few root knots are connected with each other; 3 stages: root knots are arranged on 31-50% of root systems, and half root knots are connected with each other; 4 stages: root knots are arranged on 51-75% of root systems, and a plurality of root knots are connected with each other; 5 stages: root knots are formed on more than 75% of root systems, the roots become thicker and malformed.
2. Test results
2.1 dynamic changes in 2-year larvae of root knot nematode
After cucumber transplanting for 11 days, the quantity of root-knot nematodes J2 in the soil treated by 10% fosthiazate and 0.5% avermectin emulsifiable concentrate is lower than that of shale soil disease-inhibiting matrix. After 35d of transplanting, the J2 quantity in the shale soil disease-suppressing matrix treated soil is obviously lower than that of 10% fosthiazate and 0.5% avermectin emulsifiable concentrate. After 65d transplanting, the shale soil disease-suppressing matrix (200 g/cave) J2 number 91, RI of 1.33, is significantly lower than that of the medicament and blank control treatment, and the root knot nematode number difference between the treatments of the shale soil disease-suppressing matrix is not significant, as shown in Table 1. The results show that the shale soil disease-inhibiting matrix microbiome can form a microbial beneficial strain group around the rhizosphere of planted cucumbers, a biological protection barrier is established, the number of root-knot nematodes is continuously reduced, and the root-knot nematode propagation index is effectively reduced.
TABLE 1 different treatments of different phases of the population dynamics of the root-knot nematode J2
The lower case letters after the same row of numbers indicate significant differences at the 0.05 level, as follows.
2.2 effects of shale-soil disease-suppressing matrix on root node progression and root node disease index
The measurement result after 65d of cucumber transplanting shows (Table 2) that the shale soil disease-inhibiting matrix treatment has obvious root knot nematode prevention effect, the difference among the treatments is not obvious, wherein the root knot progression and the root knot disease index of the shale soil disease-inhibiting matrix (200 g/hole) treatment are respectively 2.0 and 20.1, and the prevention effect on the root knot nematode reaches 79.4 percent and is obviously higher than that of other medicament treatment prevention effects.
TABLE 2 influence of different treatments on the disease index and control effect of cucumber root knot
The different lower case letters following the same row of numbers indicate significant differences at the 0.05 level.
Application example 3
Shale soil disease-suppressing matrix field cucumber fusarium wilt prevention and control test
1. Test site and method
Experimental location and experimental method referring to application example 2, disease resistance indexes include plant morbidity, disease index and control effect. Melon wilt grading standard, grade 0: no symptoms; stage 1: the yellowing or wilting area of the true leaves and the cotyledons is not more than 50% of the total area; 2 stages: the yellowing or wilting area of the true leaves and the cotyledons exceeds 50% of the total area; 3 stages: leaf wilting or withering, only growing points survive; 4 stages: the whole plant seriously wilts, so that the whole plant dies. The disease index is calculated by Zong Zhaofeng, wherein the disease index is = Σ (number of disease stage plants×number of representing stages)/(total number of plants×highest representing stage value) ×100; control effect (%) = (control disease index-treatment disease index)/control disease index x 100.
2. Test results
The measurement result after 65d of cucumber transplanting shows that the incidence, incidence degree, control effect and control of cucumber plants treated by the shale soil disease-suppressing matrix have significant differences, and the table 3 shows. Most of the diseased plants in the control group have leaf wilting and stem constriction with water stain leaching, vascular bundles are cracked and brown, grow slowly or die, and the incidence rate is up to 38.7%. The cucumber plants treated by the shale soil disease-suppressing matrix (200 g/cave) grow rapidly, the stems are thick, the diseased plants are fewer, the disease degree is weaker, the disease degree is only 10.2%, the control effect on cucumber fusarium wilt reaches 88.6%, and the control effect is remarkably higher than that of the medicament treatment.
TABLE 3 inhibition effect of different treatments on cucumber fusarium wilt
Therefore, the shale soil disease-inhibiting matrix provided by the embodiment has remarkable effect in preventing and controlling cucumber fusarium wilt, constructs disease-inhibiting rhizosphere soil micro-ecological environment, inhibits plant diseases and insect pests and potential pathogenic bacteria of animals, plants, people and animals, further rapidly obtains soil immunity in the same season of crops, realizes stable field control effect and accurate targeted regulation of soil health of the soil-borne diseases of the facility vegetables, stimulates plant growth, relieves soil hardening, improves soil ecological environment and the like, effectively reduces the formation and aggravation problems of continuous cropping obstacles of the facility vegetables, and has good economic benefit.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The shale soil disease-suppressing matrix is characterized by comprising the following components in percentage by mass: 40 to 60 percent of shale soil, 5 to 15 percent of vermiculite, 10 to 20 percent of rice bran, 2.5 to 7.5 percent of humic acid, 1.5 to 3.5 percent of industrial brown sugar and 5 to 10 percent of microorganism composite bacteria are mixed and fermented to prepare the fertilizer;
wherein the microorganism complex bacteria comprise ferment bacteria propagation strains, 10.0x10 8 cfu/g lilyturf-pt 361 spore liquid, 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquidThe method comprises the steps of carrying out a first treatment on the surface of the The components in percentage by mass are as follows: 3 to 7 percent of ferment bacteria propagation strain, 0.25 to 0.75 percent of 10.0x10 8 cfu/g of lilyturf-pt 361 spore liquid, 1-3% of 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid;
the shale soil disease-suppressing matrix is applied to construction of a disease-suppressing rhizosphere soil micro-ecological environment, and the shale soil disease-suppressing matrix generates three microbial beneficial flora of bacteria, fungi and actinomycetes in soil;
the shale soil disease-inhibiting matrix is used for inhibiting the harm of root-knot nematodes.
2. The shale soil conditioning substrate of claim 1, wherein the shale soil conditioning substrate comprises the following components in mass percent: 60% shale soil, 10% vermiculite, 15% rice bran, 5% humic acid, 2.5% industrial brown sugar and 7.5% microorganism composite bacteria.
3. The shale soil disease-suppressing matrix of claim 2, wherein the microorganism complex comprises the following components in percentage by mass: 5% of ferment bacteria propagation strain, 0.5% of 10.0X10 8 cfu/g lilyturf-pt 361 spore liquid, 2% 10.0X10 8 cfu/g bacillus bailii NH-1 bacterial liquid.
4. A method for preparing the shale soil disease suppressing matrix as claimed in any one of claims 1 to 3, comprising the steps of:
firstly, weighing shale soil, vermiculite, rice bran, humic acid, industrial brown sugar and microorganism composite bacteria according to mass percentage;
step two, uniformly mixing the microorganism complex bacteria and rice bran, spraying the prepared brown sugar water onto the microorganism complex bacteria and rice bran mixture, stirring while spraying, and adjusting the water content to be 45-55%;
uniformly scattering the mixture obtained in the step two, humic acid and vermiculite on shale soil, stirring while scattering, and regulating the water content to be 45-55%;
piling the mixture prepared in the step three on a clean panel, sieving, piling up a mountain shape with the height of about 1.2m, and covering for heat preservation;
turning the pile: after 48 hours from the date of manufacture, turning up and down and piling up into a mountain shape, and turning up 1 time every day;
step six, drying: spreading on the clean panel for natural drying or warm air drying;
and step seven, checking, sieving and packaging.
5. The method for preparing shale soil disease-suppressing matrix as recited in claim 4, wherein in the fifth step, the turning is performed at the temperature of 50-55 ℃.
6. The method for preparing shale soil disease-suppressing matrix as recited in claim 4, wherein the step five is performed 6 times.
7. The method for preparing shale soil disease-suppressing matrix as recited in claim 4, wherein the moisture content of the mixture after air drying in the sixth step is 5-10%.
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