CN114981427A - 标签化的转座复合体及其在高通量测序中的应用 - Google Patents
标签化的转座复合体及其在高通量测序中的应用 Download PDFInfo
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Abstract
标签化的转座复合体及其在高通量测序中的应用。提供了一种转座酶识别元件，为如下结构：X(m)Y(f)N(n)；X(m)表示转座酶识别区，为双链核酸结构；Y(f)表示间隔区，为单链DNA结构；N(n)表示样本标签，为单链DNA结构。高分子量DNA采用标签化的转座复合体进行处理后，得到大量标签化的DNA片段。将各个高分子量DNA得到的标签化DNA片段混合，得到混合样本；采用具有分子标签的载体进行捕获；采用核酸外切酶进行处理，然后释放转座酶；采用stLFR技术进行建库，得到DNA文库。可用于高通量测序平台混合测序。
Description
PCT国内申请，说明书已公开。
Claims (19)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2020/090790 WO2021232184A1 (zh) | 2020-05-18 | 2020-05-18 | 标签化的转座复合体及其在高通量测序中的应用 |
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| CN114981427A true CN114981427A (zh) | 2022-08-30 |
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Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20230174969A1 (zh) |
| CN (1) | CN114981427A (zh) |
| WO (1) | WO2021232184A1 (zh) |
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-
2020
- 2020-05-18 WO PCT/CN2020/090790 patent/WO2021232184A1/zh active Application Filing
- 2020-05-18 US US17/925,157 patent/US20230174969A1/en active Pending
- 2020-05-18 CN CN202080093546.XA patent/CN114981427A/zh active Pending
Patent Citations (9)
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| CN106715693A (zh) * | 2014-06-13 | 2017-05-24 | 伊卢米纳剑桥有限公司 | 用于制备序列文库的方法和组合物 |
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| US20200056224A1 (en) * | 2017-04-18 | 2020-02-20 | Fred Hutchinson Cancer Research Center | Barcoded transposases to increase efficiency of high-accuracy genetic sequencing |
| CN110678547A (zh) * | 2017-05-23 | 2020-01-10 | 生物辐射实验室股份有限公司 | 分子条码化 |
| CN109526228A (zh) * | 2017-05-26 | 2019-03-26 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| WO2019217452A1 (en) * | 2018-05-08 | 2019-11-14 | Mgi Tech Co., Ltd. | Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly |
| CN111041563A (zh) * | 2019-12-31 | 2020-04-21 | 广州精科医学检验所有限公司 | 一种靶向序列捕获及pcr建库方法 |
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| WO2021232184A1 (zh) | 2021-11-25 |
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