CN114946954A

CN114946954A - Solid beverage of probiotic compound composition containing traditional Chinese medicine components and preparation method

Info

Publication number: CN114946954A
Application number: CN202110218962.4A
Authority: CN
Inventors: 邱淦清; 邢俊波; 魏振
Original assignee: Huzhou Xinhuo Medical Technology Co ltd
Current assignee: Huzhou Xinhuo Medical Technology Co ltd
Priority date: 2021-02-26
Filing date: 2021-02-26
Publication date: 2022-08-30

Abstract

The invention relates to the technical field of probiotic food, in particular to a solid beverage of a probiotic compound composition containing traditional Chinese medicine components and a preparation method thereof. A solid beverage of probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials: probiotic composition, inulin, fructo-oligosaccharide, folium Mori powder or rhizoma Dioscoreae powder, and Coicis semen powder. The preparation method comprises the following steps: the qualified raw materials in percentage by mass are weighed, pretreated, subjected to superfine grinding, mixed, packaged in bags, externally packaged, inspected and warehoused. According to the invention, probiotics and traditional Chinese medicines are organically compatible, a creative formula is provided, and through mutual coordination of mulberry leaf powder or yam powder, coix seed powder and a probiotic composition, the mild conditioning effect of the traditional Chinese medicines can be exerted, and meanwhile, the biological activity of the probiotics is nourished and excited, so that the combination is strong, and the advantages are complementary.

Description

Solid beverage of probiotic compound composition containing traditional Chinese medicine components and preparation method

Technical Field

The invention belongs to the technical field of probiotic food, and particularly relates to a solid beverage of a probiotic compound composition containing traditional Chinese medicine components and a preparation method thereof.

Background

Probiotics are one of the dietary supplements preferred by consumers at present and have an important position in modern life. Probiotics are active microorganisms that act by improving the balance of the host intestinal microflora. The probiotics have the effects of improving the intestinal flora structure, inhibiting pathogenic bacteria, eliminating carcinogenic factors, improving the immunity of the organism, reducing cholesterol and the like. In medicine, the probiotics can be used for treating diarrhea, constipation and colon dysfunction caused by acute rotavirus and side effects caused by radiotherapy, and can also be used for resisting tumors and cancers. The probiotic agent has no toxicity, residue, pollution and drug resistance, so that flora imbalance and superinfection caused by using antibiotics can be overcome, and adverse consequences caused by abuse of antibiotics and the like can be avoided; after the antibiotics are used, the probiotics are used to obviously regulate and promote the restoration of the micro-ecological balance of the animal body. On the other hand, the number of probiotics is continuously reduced because the human body gradually ages. The supplement of probiotics can further improve the 'body defense ability' to be beneficial to the prevention of diseases, so that the provision of healthy and nutritional probiotics plays a very critical role in the health and life of the human body.

CN 110354152 a discloses a probiotic composition for reducing blood lipid, comprising lyophilized bacteria powder, polysaccharide, polyphenol and polypeptide. CN 110432328A discloses a probiotic and a preparation method thereof, which comprises the following components: the lactobacillus acidophilus freeze-dried powder, the bifidobacterium animalis freeze-dried powder, the milk powder, the inulin, the mannitol and the silicon dioxide are mixed and stirred uniformly to obtain the lactobacillus acidophilus freeze-dried powder.

On one hand, the formula of the probiotic bacteria is the combination of traditional probiotics and prebiotics, and the effect is relatively single; on the other hand, the species and number of the strains of the probiotics used are relatively limited, which affects the effectiveness thereof.

Disclosure of Invention

Aiming at the defects in the technology, the invention adopts the combination formula of the traditional Chinese medicine raw materials with homology of medicine and food and the probiotics, solves the efficiency restriction of the probiotics in the application process in the prior art, and achieves the essential attribute of safe and efficient food therapy.

In order to achieve the purpose, the invention provides the following technical scheme:

the invention provides a solid beverage of a probiotic compound composition containing traditional Chinese medicine components, which is prepared from the following raw materials: : probiotic composition, inulin, fructo-oligosaccharide, folium Mori powder or rhizoma Dioscoreae powder, and Coicis semen powder.

Preferably, the mass percentages of the components are as follows: 14-26% of probiotic composition, 31-36% of inulin, 14-26% of fructo-oligosaccharide, 4-16% of mulberry leaf powder or yam powder and 1-10% of coix seed powder, wherein the sum of the mass percentages of the components is 100%.

Preferably, the solid beverage comprises any one or more of blueberry fruit powder, skim milk powder and erythritol so as to increase the mouthfeel of the solid beverage.

Preferably, the mass percentages of the components are as follows: 14-16% of probiotic composition, 31-33% of inulin, 14-16% of fructo-oligosaccharide, 4-6% of mulberry leaf powder or yam powder, 1-3% of coix seed powder, 1-3% of blueberry fruit powder, 19-21% of skim milk powder and 9-11% of erythritol, wherein the sum of the mass percentages of the components is 100%.

Preferably, the probiotic composition comprises: bifidobacterium BLa80, Bifidobacterium longum BL, Lactobacillus bulgaricus, Lactobacillus plantarum LP90, Bifidobacterium bifidum, and Lactobacillus rhamnosus, and the effective viable count of each strain is not less than 3 × 10 ¹⁰ CFU/g。

Preferably, the raw material of the mulberry leaf powder is young mulberry buds of the first crop before Qingming.

Preferably, the mixture is ultrafine powder.

Preferably, the particle size of the superfine powder is not more than 75 μm.

More preferably, the particle size of the ultrafine powder is 25 to 58 μm.

Preferably, the solid beverage is in the form of granules, powder or a mixture of granules and powder.

The invention also provides a preparation method of the solid beverage of the probiotic compound composition containing the traditional Chinese medicine components, which comprises the following steps:

the method comprises the following steps:

step 1, pretreatment of traditional Chinese medicine components: drying and crushing mulberry leaves or Chinese yam and coix seeds, and sieving the crushed mulberry leaves or Chinese yam and coix seeds by a 24-50-mesh sieve to obtain fine powder for later use;

step 2, pretreatment of auxiliary materials: drying inulin, fructo-oligosaccharide, blueberry fruit powder, skim milk powder and erythritol, and sieving with a 24-50-mesh sieve for later use;

step 3, crushing the traditional Chinese medicine components: drying the fine powder obtained in the step 1 at a low temperature of-20 to-10 ℃, and then carrying out ultra-low temperature ultra-micro crushing at a temperature of-35 to-30 ℃ to obtain ultra-micro powder for later use;

step 4, sieving the superfine powder obtained in the step 3, wherein the particle size of the superfine powder is not more than 75 microns;

step 5, weighing: weighing the inulin, the fructo-oligosaccharide, the blueberry fruit powder, the skim milk powder and the erythritol which are treated in the step 2 according to the mass parts, weighing the mulberry leaf powder or the yam powder and the coix seed powder which are treated in the step 4 according to the mass parts, and weighing the probiotic composition according to the mass parts for later use;

step 6, mixing: and (5) uniformly stirring the components weighed in the step (5) to obtain the traditional Chinese medicine probiotic compound composition solid beverage.

Preferably, the particle size of the superfine powder is 25-58 μm.

The invention has the following beneficial effects: (1) the formula adopts the innovative combination of medicinal and edible traditional Chinese medicine raw materials of mulberry leaves, coix seeds and probiotics, so that the synergistic effect is better exerted, and the effect is milder and more lasting; (2) the raw materials are prepared by an ultralow-temperature superfine grinding wall-breaking technology, the particle size reaches 25-58 mu m, the dissolution rate and absorption of active ingredients are faster and more efficient, and the originality of the basis of effective substances and the integrity of all components are retained to the maximum extent; (3) six-linked active strains are compounded and combined, so that the intestinal flora balance is professionally adjusted, and the intestinal toxins and garbage are effectively removed; (4) the solid particles are adopted, so that the carrying and the application are more convenient. (5) Through the mutual coordination of the mulberry leaves or the Chinese yam, the coix seeds, the probiotic composition and the prebiotics, the stomach mucosa is protected to achieve the function of conditioning the stomach and intestine, the functions of reducing blood pressure and blood fat and inhibiting helicobacter pylori are achieved, and the health-care efficacy of medicine and food dual-purpose is fully exerted when the stomach ache, the stomach heartburn and the diarrhea are rapidly relieved. Experiments show that the solid beverage provided by the invention not only enables the prebiotics and the probiotics to generate a synergistic effect, but also enables the mulberry leaves or the Chinese yam, the coix seeds, the prebiotics and the probiotics to generate a synergistic effect, so that the composition has a quicker and more remarkable effect on relieving stomach discomfort.

The source and the function of the raw materials of the traditional Chinese medicine components are as follows:

folium Mori is derived from dried leaves of Morus alba L of Moraceae. The Chinese pharmacopoeia records: mulberry leaf, mulberry leaf, radix Et rhizoma Rhei, is sweet, bitter and cold in flavor. It enters lung and liver meridians. [ FUNCTIONS ] can dispel wind-heat, clear lung-heat, moisten dryness, clear liver and improve eyesight. Can be used for treating wind-heat type common cold, lung heat type cough, dizziness, headache, conjunctival congestion, and dim eyesight. The mulberry leaves are medicinal and edible raw materials, contain flavone, flavonoid glycoside, alkaloid, polysaccharide, sterol, volatile oil, amino acid and vitamin, have the functions of reducing blood sugar, blood fat and atherosclerosis, resisting inflammation, aging, tumor, virus, filarial worm, ulcer and constipation [ Wuxianan, Chenjie, Wanjiali pear, and the like ] and have the regulation function of the fermented lotus seed milk on the gastrointestinal tract movement and absorption functions of mice. 2535-2539.].

The raw material mulberry leaf is preferably young mulberry bud of the first crop before Qingming, and the nutrition and medicinal value are higher.

Rhizoma Dioscoreae is derived from dried rhizome of Dioscorea opposita Thunb of Dioscoreaceae. The Chinese pharmacopoeia records: chinese yam, rhizoma Dioscoreae (nature and taste) is sweet and neutral. It enters spleen, lung and kidney meridians. [ FUNCTIONS ] can tonify spleen and stomach, promote the production of body fluid and nourish lung, tonify kidney and arrest seminal emission. Can be used for treating spleen deficiency, anorexia, chronic diarrhea, lung deficiency, cough, asthma, spermatorrhea, leukorrhagia, frequent micturition, and diabetes due to deficiency heat. The Chinese yam has rich nutritive value, is a well-known nourishing good product, is one of plants with dual purposes of medicine and food published by the Ministry of health, and is also an important raw material of health-care food. It contains abundant proteins, amino acids essential to human body, various trace elements, polysaccharide, allantoin, choline, sterol, and other nutritional components and functional factors. The Chinese yam has the effects of strengthening spleen, tonifying lung, reinforcing kidney and replenishing vital essence, and is a good spleen-invigorating and qi-tonifying medicine commonly used in traditional Chinese medicine. Modern pharmacological experiments show that the Chinese yam has a plurality of remarkable effects of reducing blood sugar and blood fat, resisting oxidation and aging, regulating the spleen and stomach, protecting the liver, regulating immunity, resisting tumors and the like [ Dongqinghai, Wufulin, Wangqun, and the like. 98-103.].

Coix seed: is dried mature kernel of Coicis semen (Coix lacryma-jobi L. var. ma-yuen (Roman.) Stapf.) of Gramineae. The Chinese pharmacopoeia records: semen coicis (nature and taste) is sweet, light and cool; it enters spleen, stomach and lung meridians. [ FUNCTIONS ] can promote diuresis, eliminate dampness, invigorate spleen, arrest diarrhea, remove arthralgia, expel pus, remove toxicity, and dissipate stagnation. Can be used for treating edema, loempe, dysuria, spleen deficiency, diarrhea, damp arthralgia, spasm, pulmonary abscess, intestinal abscess, wart, and cancer. Semen Coicis contains fatty acids and esters, coixol, saccharides, flavonoids, glycoproteins, sterols, and lactams. Modern pharmacological studies show that coix seeds have various pharmacological activities such as anti-tumor, anti-inflammatory, analgesic, blood glucose and blood pressure reduction, and immunity improvement [ pichen, yangonin, mohachun. 52-56].

The sources and functions of the other raw materials except the traditional Chinese medicine component are as follows:

inulin: the inulin composition has the effects of reducing blood sugar value, reducing blood sugar concentration, enhancing digestion and metabolism, improving vascular disorder and the like, is low in energy, meets the requirements of weight reduction and health maintenance, is a proliferation factor of bifidobacterium, can greatly increase beneficial bacteria in colon by eating inulin every day, and reduces pathogenic bacteria and putrefying bacteria.

Fructo-oligosaccharide: the bifidobacterium bifidum health-care food is an all-natural ingredient simultaneously having the dual-physiological characteristics of the super-strong bifidobacterium factor and the water-soluble dietary fiber, has small molecular weight and has a proliferation effect on the health-care effect of bifidobacterium; the addition of the fructo-oligosaccharide not only improves the nutrition and health care value of the product, but also can effectively prolong the shelf life of the product; in addition, the fructo-oligosaccharide has low calorie, does not cause obesity and can not cause blood sugar rise, and is an ideal novel health-care sweetener; the fructo-oligosaccharide can promote the growth of bifidobacteria, lactobacilli and the like in colon, can meet the basic physiological functions and metabolism requirements of human bodies, can promote the health of the human bodies, and has the effects of supplementing each other and bringing out the best in each other.

Blueberry fruit powder: contains anthocyanidin, vegetable protein, vitamin C, vitamin E, carotene, citric acid, malic acid, salicylic acid, amino acids, dietary fiber, and minerals such as calcium, phosphorus, and ferrum. It has good nutrition and health promotion effects, and also has effects of preventing cranial nerve aging, tonifying heart, resisting cancer, softening blood vessel, and enhancing immunity.

Skim milk powder: because of its low fat content, it is easy to preserve, difficult to produce oxidation, it is a very good food material. The defatted milk powder contains no fat, and is suitable for people with obesity and need for nutrition supplement. It is beneficial for the elderly, infants with dyspepsia, and patients with diarrhea, gallbladder diseases, hyperlipidemia, and chronic pancreatitis.

Erythritol: the health food has the characteristics of being not easily degraded by enzyme, not participating in sugar metabolism and not causing blood sugar change, and is suitable for the application of the health food for diabetics; the health food with low energy value is prepared by replacing cane sugar, and is suitable for obese people, hypertension patients and cardiovascular patients; the metabolic characteristic in intestinal tract after eating is suitable for people with gastrointestinal disorder.

Bifidobacterium BLa 80: can inhibit the reproduction of harmful bacteria in intestine, reduce toxin, promote intestinal peristalsis, improve intestinal function, and improve defecation. Not only can inhibit the generation of harmful flora in the intestine, but also can provide good growth environment for beneficial bacteria in the intestine, thereby creating healthy intestinal tract.

Bifidobacterium longum BL 21: medical research proves that the bifidobacterium longum BL has the effects of regulating the intestinal tract of a human body and improving health.

Lactobacillus bulgaricus: firstly, the lactose can be fermented, galactose and glucose generated by fermentation are easy to absorb, the development of brain and nerves can be promoted, the emptying of gastric contents is promoted, the hypersecretion of gastric acid is reduced, the utilization rate of calcium, phosphorus and iron is improved, and harmful bacteria in intestinal tracts are inhibited. Secondly, it can decompose milk protein into peptide and amino acid, which is more beneficial to digestion and absorption, and can improve the utilization rate of protein. In addition, the lactobacillus has certain anti-inflammatory effect by improving intestinal flora, inhibiting carcinogen generation, reducing content of indigoid substrate and phenol in intestines, activating immunologic function and inhibiting cancer cell proliferation.

Lactobacillus plantarum LP 90: relieving heavy metal toxicity, assisting blood lipid and blood pressure, relieving type ii, and inhibiting pathogenic bacteria. ,

bifidobacterium bifidum: has the effects of maintaining normal intestinal bacterial flora balance, inhibiting pathogenic bacteria growth, and preventing constipation, diarrhea, gastrointestinal disorder, etc.; anti-tumor; synthesizing vitamins and amino acids in intestinal tract and improving the absorption of calcium ions by organism; reducing blood cholesterol level, and preventing and treating hypertension; the lactose resistance of the dairy product is improved, and the digestibility is improved; enhancing immunity, resisting aging, and prolonging life. Enhancing non-specific and specific immune response of the body; control of endotoxemia; the tolerance of the host to radiation is improved.

Lactobacillus rhamnosus: the intestinal canal has high adhesion rate and strong colonization ability, has high efficiency of reducing cholesterol, promotes cell division, and has important physiological health care functions of regulating intestinal flora, preventing and treating diarrhea, eliminating toxin, preventing decayed teeth, improving immunity of organisms, resisting cancer and the like.

Further, the probiotic composition comprises one or more of bifidobacterium BLa80, bifidobacterium longum BL, lactobacillus bulgaricus, lactobacillus plantarum LP90, bifidobacterium bifidum and lactobacillus rhamnosus.

The beneficial effects of the probiotics feed additive are that a plurality of probiotics are matched and used, supplement each other and complement each other, so that the immunity of the organism can be obviously improved, and the digestion and absorption of the gastrointestinal tract can be improved.

The invention has the advantages that: the traditional Chinese medicine has the dietetic therapy view that five cereals are nourished, five fruits are helped, five animals are benefited, and five vegetables are used as supplements (the 'Huangdi's plain inquiry and Qi-storing method 'theory) and' millet flesh, fruit and vegetable and food are kept completely '(the' Huangdi's plain inquiry and five regular political major theory'); the formula has the advantages that the medicine and the food are obtained simultaneously, the traditional Chinese medicine compatibility degree of the traditional Chinese medicine theory is followed, and the pharmacological analysis of the modern traditional Chinese medicine and the relevant theory and clinical achievement of the modern medicine are combined; the formula has originality, probiotics and medicinal and edible raw materials are organically combined, the effect is mild and lasting, no additive is added, and the composition is safe and nontoxic and conforms to the essential attribute of food therapy; the process adopts superfine pulverizing process, so that the active ingredients can be quickly absorbed, and the drug effect and bioavailability can be obviously improved. Through the mutual coordination of the mulberry leaf powder or the yam powder, the coix seed powder, the probiotic composition and the prebiotics, the traditional Chinese medicine composition can play a role in mildly conditioning, nourish and stimulate the biological activity of probiotics, strongly combine and complement the advantages.

The quality of the raw materials directly determines and influences the quality and effect of the product, and the preferred mulberry bud is picked before Qingming every year, so that the yield is low, the mulberry bud is rare, and the nutrition and medicinal value are higher due to accumulation and precipitation of the mulberry bud in three stages of summer, autumn and winter. The preferred coix seeds are from the origin of 1400-micrometer elevation in southeast of Qian province, and have the advantages of fresh air, pure water source, beautiful environment, no pollution, no additive, more nutrition and more health.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. It is intended that all modifications or alterations to the methods, procedures or conditions of the present invention be made without departing from the spirit and substance of the invention.

In the following examples, the effective viable count of Bifidobacterium BLa80, Bifidobacterium longum BL, Lactobacillus bulgaricus LP90, Bifidobacterium bifidum, and Lactobacillus rhamnosus is not less than 3 × 10 ¹⁰ CFU/g；

The mulberry leaves conform to the regulation of the first edition of China pharmacopoeia 2020;

the yam conforms to the regulation of the first part of China pharmacopoeia 2020 edition;

the coix seed conforms to the regulation of the first part of China pharmacopoeia 2020 edition;

the inulin meets the regulation of 'notice on approving inulin and polyfructose as new resource food' (No. 5 in 2009) 'issued by the Committee on national health and family planning of the people's republic of China;

the fructo-oligosaccharide meets the regulation of GB/T235283;

the blueberry fruit powder meets the standard regulation of corresponding qualification production enterprises;

the skim milk powder meets the regulations of RHB 202;

erythritol meets the specifications of GB 26404;

the sweet orange essence conforms to the provisions of GB30616

Example 1

A solid beverage of a probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials in percentage by mass: the probiotic composition is 15% (wherein, bifidobacterium BLa 803%, bifidobacterium longum BL 213%, lactobacillus bulgaricus 3%, lactobacillus plantarum LP 902%, bifidobacterium bifidum 2%, lactobacillus rhamnosus 2%), inulin 32%, fructo-oligosaccharide 15%, mulberry leaf powder 5%, coix seed powder 2%, blueberry fruit powder 1%, skim milk powder 20%, erythritol 10%.

The preparation method comprises the following steps:

step 1, checking: all the raw materials are inspected according to corresponding quality standards;

step 2, pretreatment of the traditional Chinese medicine raw materials: selecting folium Mori (young sprout before Qingming) and Coicis semen, cleaning, drying (35 deg.C), pulverizing, and sieving with 24 mesh sieve to obtain fine powder;

step 3, pretreatment of auxiliary materials: drying inulin, fructo-oligosaccharide, blueberry fruit powder, skim milk powder and erythritol (35 deg.C), and sieving with 24 mesh sieve;

and 4, crushing the traditional Chinese medicine raw materials: drying the fine powder obtained in the step 2 at a low temperature of-20 ℃, then carrying out ultra-low temperature ultra-micro grinding at a temperature of-35 ℃ to obtain ultra-micro powder, and sieving to obtain the ultra-micro powder with the particle size of not more than 75 microns for later use;

step 5, weighing: weighing the above inulin, fructo-oligosaccharide, mulberry leaf powder, coix seed powder, blueberry fruit powder, skim milk powder, erythritol, bifidobacterium BLa80, bifidobacterium longum BL, lactobacillus bulgaricus, lactobacillus plantarum LP90, bifidobacterium bifidum and lactobacillus rhamnosus for later use according to the mass parts;

step 6, mixing the traditional Chinese medicine raw materials: uniformly mixing the ultrafine powder of the mulberry leaf powder weighed in the step 5 with the ultrafine powder of the coix seed powder;

step 7, mixing probiotics: mixing erythritol, Bacillus bifidus BLA80, Bifidobacterium longum BL, Lactobacillus bulgaricus LP90, and Bifidobacterium bifidum;

step 8, total mixing: mixing the mixture obtained in the step 6 and the mixture obtained in the step 7 with inulin, fructo-oligosaccharide, blueberry fruit powder, skim milk powder and erythritol, and uniformly stirring for 40 minutes to obtain the traditional Chinese medicine probiotic compound composition solid beverage;

step 9, packaging in bags: the solid beverage prepared in the step 8 is packaged in bags by an automatic powder packaging machine, and the specification of the bag packaging is 2 g/bag;

step 10, outer packaging: putting the samples packaged in the step 9 into small boxes, 20 bags/box, labeling, boxing and packaging;

step 11, checking: inspecting the finished product in the step 10 according to the product delivery inspection requirement;

and step 12, warehousing: and (5) warehousing and storing the finished product in the step (11) after the finished product is qualified.

In a preferred embodiment, the production steps from step 1 to step 9 are all performed in a production environment with 10 ten thousand-level cleanliness, so that the product hygiene index can meet the quality requirement effectively.

Example 2

A solid beverage of a probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials in percentage by mass: the probiotic composition is 14% (wherein, bifidobacterium BLa 803%, bifidobacterium longum BL 213%, lactobacillus bulgaricus 2%, lactobacillus plantarum LP 902%, bifidobacterium bifidum 2%, lactobacillus rhamnosus 2%), inulin 32%, fructo-oligosaccharide 16%, mulberry leaf powder 4%, coix seed powder 1%, blueberry fruit powder 1%, skim milk powder 21%, erythritol 11%.

The preparation method comprises the following steps:

step 3, pretreatment of auxiliary materials: drying inulin, fructo-oligosaccharide, blueberry fruit powder, skim milk powder and erythritol (50 ℃), and sieving with a 50-mesh sieve for later use;

and 4, crushing the traditional Chinese medicine raw materials: drying the fine powder obtained in the step 2 at a low temperature of-10 ℃, then carrying out ultra-low temperature ultra-fine grinding at a temperature of-30 ℃ to obtain ultra-fine powder, and sieving to obtain the ultra-fine powder with the particle size of not more than 75 microns for later use;

step 9, packaging in bags: the solid beverage prepared in the step 8 is packaged in bags by an automatic powder packaging machine, wherein the specification of the bag packaging is 2 g/bag;

In a preferred embodiment, the production steps from step 1 to step 9 are all performed in a production environment with cleanliness of 10 ten thousand level, so that the sanitation index of the product can meet the quality requirement.

Example 3

A solid beverage of a probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials in percentage by mass: the probiotic composition is 16% (wherein, bifidobacterium BLa 803%, bifidobacterium longum BL 213%, lactobacillus bulgaricus 3%, lactobacillus plantarum LP 903%, bifidobacterium bifidum 2%, lactobacillus rhamnosus 2%), inulin 31%, fructo-oligosaccharide 14%, mulberry leaf powder 6%, coix seed powder 3%, blueberry fruit powder 2%, skim milk powder 19%, erythritol 9%.

The preparation method comprises the following steps:

step 2, pretreatment of the traditional Chinese medicine raw materials: selecting folium Mori (young sprout before Qingming) and Coicis semen, cleaning, drying (at 40 deg.C), pulverizing, and sieving with 24 mesh sieve to obtain fine powder;

step 3, pretreatment of auxiliary materials: drying inulin, fructo-oligosaccharide, blueberry fruit powder, skim milk powder and erythritol (40 ℃), and sieving with a 45-mesh sieve for later use;

step 6, mixing the traditional Chinese medicine raw materials: mixing the superfine powder of the mulberry leaf powder weighed in the step 5 and the superfine powder of the coix seed uniformly,

Example 4

A solid beverage of a probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials in percentage by mass: the probiotic composition is 15% (wherein, bifidobacterium BLa 803%, bifidobacterium longum BL 213%, lactobacillus bulgaricus 3%, lactobacillus plantarum LP 902%, bifidobacterium bifidum 2%, lactobacillus rhamnosus 2%), inulin 33%, fructo-oligosaccharide 14%, mulberry leaf powder 5%, coix seed powder 2%, blueberry fruit powder 3%, skim milk powder 19%, erythritol 9%.

The preparation method comprises the following steps:

Example 5

A solid beverage of a probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials in percentage by mass: the probiotic composition is 15% (wherein, bifidobacterium BLa 803%, bifidobacterium longum BL 213%, lactobacillus bulgaricus 3%, lactobacillus plantarum LP 902%, bifidobacterium bifidum 2%, lactobacillus rhamnosus 2%), inulin 32%, fructo-oligosaccharide 15%, yam powder 5%, coix seed powder 2%, blueberry fruit powder 1%, skim milk powder 20%, erythritol 10%.

The preparation method comprises the following steps: the preparation method comprises the following steps:

step 2, pretreatment of the traditional Chinese medicine raw materials: selecting and cleaning raw materials of Chinese yam and coix seed, drying (at 35 ℃), crushing, and sieving by a 24-mesh sieve to obtain fine powder for later use;

step 5, weighing: weighing inulin, fructo-oligosaccharide, yam powder, coix seed powder, blueberry fruit powder, skim milk powder, erythritol, bifidobacterium BLA80, bifidobacterium longum BL, lactobacillus bulgaricus, lactobacillus plantarum LP90, bifidobacterium bifidum and lactobacillus rhamnosus for later use according to the mass parts;

step 6, uniformly mixing the ultrafine powder of the yam powder weighed in the step 5 and the ultrafine powder of the coix seed powder;

step 8, total mixing: mixing the mixture obtained in the step 6, the mixture obtained in the step 7, inulin, fructo-oligosaccharide, blueberry fruit powder, skim milk powder and erythritol, and uniformly stirring (40 minutes) to obtain the traditional Chinese medicine probiotic compound composition solid beverage;

Example 6

The preparation method comprises the following steps:

step 2, pretreatment of the traditional Chinese medicine raw materials: selecting and cleaning raw materials including Chinese yam and coix seed, drying (at 35 ℃), crushing, and sieving by a 24-mesh sieve to obtain fine powder for later use;

step 5, weighing: weighing the above inulin, fructo-oligosaccharide, yam powder, coix seed powder, blueberry fruit powder, skim milk powder, erythritol, bifidobacterium BLa80, bifidobacterium longum BL, lactobacillus bulgaricus, lactobacillus plantarum LP90, bifidobacterium bifidum and lactobacillus rhamnosus for later use according to the mass parts;

step 6, mixing the traditional Chinese medicine raw materials: uniformly mixing the superfine powder of the yam powder weighed in the step 5 with the superfine powder of the coix seed powder;

Example 7

A solid beverage of a probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials in percentage by mass: the probiotic composition is 16% (wherein, bifidobacterium BLa 803%, bifidobacterium longum BL 213%, lactobacillus bulgaricus 3%, lactobacillus plantarum LP 903%, bifidobacterium bifidum 2%, lactobacillus rhamnosus 2%), inulin 31%, fructo-oligosaccharide 14%, yam flour 6%, coix seed powder 3%, blueberry fruit powder 2%, skim milk powder 19%, erythritol 9%.

The preparation method comprises the following steps:

step 2, pretreatment of the traditional Chinese medicine raw materials: selecting and cleaning raw materials including rhizoma Dioscoreae and Coicis semen, drying (at 40 deg.C), pulverizing, and sieving with 24 mesh sieve to obtain fine powder;

and 4, crushing the traditional Chinese medicine raw materials: drying the fine powder obtained in the step 2 at a low temperature of-15 ℃, then carrying out ultra-low temperature ultra-fine grinding at a temperature of-33 ℃ to obtain ultra-fine powder, and sieving to obtain the ultra-fine powder with the particle size of not more than 75 microns for later use;

step 6, mixing the traditional Chinese medicine raw materials: mixing the superfine powder of the yam flour weighed in the step 5 and the superfine powder of the coix seed uniformly,

Example 8

A solid beverage of a probiotic compound composition containing traditional Chinese medicine components is prepared from the following raw materials in percentage by mass: the probiotic composition is 15% (wherein, bifidobacterium BLa 803%, bifidobacterium longum BL 213%, lactobacillus bulgaricus 3%, lactobacillus plantarum LP 902%, bifidobacterium bifidum 2%, lactobacillus rhamnosus 2%), inulin 33%, fructo-oligosaccharide 14%, yam powder 5%, coix seed powder 2%, blueberry fruit powder 3%, skim milk powder 19%, erythritol 9%.

The preparation method comprises the following steps: step 1, checking: all the raw materials are inspected according to corresponding quality standards;

The solid beverage of the probiotic compound composition containing the traditional Chinese medicine components prepared in the embodiments 1 to 8 is respectively subjected to: 1. pilot test; 2. sensory index detection test; 3. physical and chemical indexes and microorganism indexes.

1. Pilot test:

the results of pilot scale production were shown in tables 1 and 2, based on the formulation ratios listed in examples 1 to 8.

TABLE 1 Pilot test results

TABLE 2 Pilot test results

As can be seen from tables 1 and 2, the solid beverage of the probiotic compound composition containing the traditional Chinese medicine components meets the requirements of enterprise quality standards, the yield meets the regulations, and the production process of the product is reasonable.

2. And (3) sensory index inspection:

1) a small amount of the solid beverage of the probiotic compound composition containing the traditional Chinese medicine components prepared in the embodiments 1-8 is respectively taken and subjected to sensory index inspection according to GB/T29602-2013 (solid beverage) standards, and the operation steps are as follows: a50 mL sample was poured into a colorless transparent container and placed in a bright place, and the state, color, smell and taste were observed, and the results are shown in Table 3.

TABLE 3 sensory index test results

TABLE 4 sensory index test results

As can be seen from tables 3 and 4, the state, color, smell and taste of the solid beverage of the probiotic compound composition containing traditional Chinese medicine components all meet corresponding indexes.

3. Physical and chemical index and microorganism index test

Physical and chemical index inspection: a small amount of each of the probiotic composition-containing solid beverages prepared in examples 1 to 8 was subjected to physical and chemical index tests, and the results are shown in tables 5 and 6. The method comprises the steps of detecting the moisture content according to a GB 5009.3 detection method, detecting the lead content according to a GB 5009.12 detection method, detecting other pollutant limit amount according to a GB 2762 detection method, and detecting mycotoxin limit amount according to a GB 2761 detection method.

And (3) microorganism index detection: a small amount of the solid beverage of the probiotic compound composition containing the traditional Chinese medicine components prepared in the embodiments 1-8 is taken, and the results of the microbial indicator tests are shown in tables 6 and 5. The method for testing the coliform group bacteria comprises the following steps: GB 4789.3MPN counting method, and the mould testing method is GB 4789.15.

TABLE 5 results of physical and chemical indicators and microbiological indicators test

TABLE 6 test results of physicochemical and microbiological indices

As can be seen from tables 5 and 6, the solid beverage containing the probiotic compound composition containing the traditional Chinese medicine components has the water content, the total number of lactic acid bacteria, total arsenic, coliform group bacteria and staphylococcus aureus which meet the corresponding standards, and lead, mould and salmonella are not detected.

And (4) experimental conclusion: through the pilot-scale verification results of experimental examples, all quality indexes of the solid beverage of the probiotic compound composition containing the traditional Chinese medicine components meet the requirements of enterprise quality standards, the yield meets the regulations, and the production process of the product is proved to be reasonable. Through sensory index detection experiments, physicochemical indexes and microbial index detection experiments, all indexes in the obtained probiotic product accord with national standards, and the probiotic product not only has extremely high nutrient content, but also can be eaten at ease.

The following describes the application effect of the product:

constipation clinical effect observation of solid beverage containing probiotic compound composition containing traditional Chinese medicine components

1. Clinical data

300 constipation patients were selected as subjects. The selected patients were included in the treatment group 240 and the control group 60 in the order of treatment using a random number table. The general data of sex, age, course of disease, etc. of two groups of patients have no obvious difference, P is more than 0.05, and the statistical significance is avoided, and the groups have comparability.

2. Diagnostic criteria

The traditional Chinese medicine diagnosis and syndrome differentiation standards and bases are as follows: according to the guideline of clinical research on constipation caused by new traditional Chinese medicine treatment, the standard of syndrome differentiation of heat secret syndrome: dry stool, difficult defecation, prolonged time interval, anal fissure, hemafecia, bitter taste in the mouth, scanty and dark urine, red tongue with yellow coating, and slippery and rapid pulse.

Western diagnosis standard and basis: according to the guideline for clinical research on novel Chinese medicine for treating constipation, the constipation diagnosis standard is as follows: (1) the defecation time is prolonged, and the interval of each defecation is more than 72 hours; (2) the stool is dry and hard, even like the sheep feces or the lump, the defecation is strenuous or the stool is not dry and hard to be discharged. Functional constipation can be diagnosed by eliminating organic lesions through necessary examination. Functional constipation is more than one month and can be brought into test cases according with the heat syndrome of traditional Chinese medicine.

3. Case exclusion criteria: (1) the examination proves that the intestinal canal is caused by rectal and colonic organic lesions (such as tumor, Crohn's disease, colon polyp, intestinal tuberculosis, etc.). (2) Those under 18 years of age, pregnant or lactating women, are allergic to the medicine. (3) Combined with serious primary lesions of cardiovascular, liver, kidney and hematopoietic systems, patients with psychosis. (4) If the composition does not meet the inclusion criteria and is not administered according to the prescription, the curative effect or the data are not complete and the curative effect and safety are not judged.

4. The test method comprises the following steps: (1) dosage and treatment course: example 1 was used for treatment group 1, example 2 was used for treatment group 2, example 3 was used for treatment group 3, example 4 was used for treatment group 4, example 5 was used for treatment group 5, example 6 was used for treatment group 6, example 7 was used for treatment group 7, and example 8 was used for treatment group 8, 2 times/d, 2 g/time, and consecutive 5 days. The control group is orally administered with fructus Cannabis pill 1 time per day, 6g each time, and continuously administered for 5 days. The treatment effect of each group is compared after 1 treatment course.

5. Observation index

(1) And (4) safety observation: general physical examination items, blood routine, urine routine, blood GPT, blood BUN, electrocardiogram.

(2) And (3) observation of curative effect: the defecation interval time, the defecation quality, the defecation labor-consuming condition and the defecation regularity.

6. Method for evaluating therapeutic effect

(1) The therapeutic effect standard and the basis thereof are as follows: according to the guidelines of clinical research on quality of new Chinese medicines for treating constipation.

(2) Evaluation standard of constipation curative effect: the clinical recovery: normal stool, all other symptoms disappeared. Secondly, effect is displayed: constipation is obviously improved, and the interval time and the stool quality are close to normal; or the stool is burnt dry and the defecation time is less than 72 hours, and most other symptoms disappear. ③ effective: the defecation interval is shortened by 1 day, or the feces quality is dried and improved, and other symptoms are all improved. Fourthly, invalidation: constipation and other symptoms were not improved.

(3) And (3) judging the traditional Chinese medicine symptom curative effect: single symptom score criteria: dry stool, 1 part in strip shape, 2 parts in block shape and 3 parts in sheep dung shape; difficult defecation, 1 minute of effort is needed, the defecation process time is prolonged by more than 1 time for 2 minutes, and 3 minutes of anal fissure bleeding are combined; the interval time is prolonged, more than 36 hours 1 min, more than 48 hours 2 min, more than 72 hours 3 min, bitter taste or dry mouth 1 min, bitter taste and dry mouth 2 min, bitter taste and dry mouth 3 min, yellow urine 1 min, short and dark color like thick tea 2 min, reddish urine 3 min; 1 part of deep red tongue, 2 parts of dry deep red tongue and 3 parts of deep red tongue; the tongue coating is thin and yellow for 1 minute, yellow and thick for 2 minutes, yellow and thick and dry for 3 minutes; the pulse condition is divided into 1 sliding part, 2 sliding parts and 3 sliding parts.

The sum of the single symptom scores is the heat syndrome score: efficacy index ═ (pre-treatment score-post-treatment score)/pre-treatment score 100%;

the clinical cure is as follows: the curative effect index is more than 85 percent;

the effect is shown: the curative effect index is more than 50 percent;

the method has the following advantages: the curative effect index is more than 20 percent;

and (4) invalidation: the curative effect index is less than 20%.

7. Statistical analysis

Analyzing and processing each data by adopting statistical software SPSS 22.0, and expressing the measurement data by adopting the mean value plus or minus standard deviation of a sample; the comparison among groups adopts t test; the comparison of the counting data and the inter-group rate adopts non-parameter test, and the difference is shown as P less than 0.05, which has statistical significance.

8. Results

The results are shown in tables 7 to 8.

TABLE 7 comparison of Constipation curative effects of the groups

The difference was statistically significant (P < 0.05).

TABLE 8 comparison of the curative effects of the heat syndrome of the Chinese medicine

The difference was statistically significant (P < 0.05).

9. Conclusion

The solid beverage containing the probiotic compound composition containing the traditional Chinese medicine components has a remarkable curative effect on constipation treatment, and no relapse of patients in a treatment group is observed in the follow-up process; in addition, no adverse reaction and toxic or side effect are found in the process of verifying the curative effect, and the preparation is worthy of clinical popularization and application.

The product is added with a probiotic composition (wherein, bifidobacterium BLa80, bifidobacterium longum BL, lactobacillus bulgaricus, lactobacillus plantarum LP90, bifidobacterium bifidum and lactobacillus rhamnosus), inulin, fructo-oligosaccharide, mulberry leaf powder or yam powder, coix seed powder, blueberry fruit powder, skim milk powder and erythritol, and the probiotic has the advantages of convenient taking, quick effect, pure taste and easy storage through the mixture ratio of the components; the preparation method has simple process, the whole process flow of the probiotics is completely sterilized without a terminal, the sanitation control in the whole production process is carried out in a production environment with 10 ten thousand grade cleanliness, the product sanitation index can effectively meet the quality requirement, and the preparation method has the advantages of simple preparation process, strong production operability and low cost, and is suitable for industrial mass production.

Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The solid beverage containing the probiotic compound composition with the traditional Chinese medicine components is characterized by being prepared from the following raw materials: probiotic composition, inulin, fructo-oligosaccharide, folium Mori powder or rhizoma Dioscoreae powder, and Coicis semen powder.

2. The solid beverage of the probiotic compound composition containing traditional Chinese medicine components according to claim 1, wherein the solid beverage comprises the following components in percentage by mass: 14-26% of probiotic composition, 31-36% of inulin, 14-26% of fructo-oligosaccharide, 4-16% of mulberry leaf powder or yam powder, 1-10% of coix seed powder, and the sum of the mass percentages of the components is 100%.

3. The solid beverage containing the probiotic compound composition containing traditional Chinese medicine components as claimed in claim 1, wherein the solid beverage comprises any one or more of blueberry fruit powder, skim milk powder and erythritol.

4. The solid beverage of the probiotic compound composition containing traditional Chinese medicine components according to claim 3, wherein the solid beverage comprises the following components in percentage by mass: 14-16% of a probiotic composition, 31-33% of inulin, 14-16% of fructo-oligosaccharide, 4-6% of mulberry leaf powder or yam powder, 1-3% of coix seed powder, 1-3% of blueberry fruit powder, 19-21% of skim milk powder and 9-11% of erythritol, wherein the sum of the mass percentages of the components is 100%.

5. The solid beverage of the probiotic compound composition containing traditional Chinese medicine components according to claims 1 to 4, characterized in that the probiotic composition comprises: one or more of Bifidobacterium BLa80, Bifidobacterium longum BL, Lactobacillus bulgaricus, Lactobacillus plantarum LP90, Bifidobacterium bifidum, and Lactobacillus rhamnosus, and the effective viable count of each strain is not less than 3 × 10 ¹⁰ CFU/g。

6. The solid beverage of the probiotic compound composition containing traditional Chinese medicine components according to any one of claims 1 to 4, characterized in that the raw material of the mulberry leaf powder is young mulberry buds of the first crop before Qingming.

7. The solid beverage of the probiotic compound composition containing traditional Chinese medicine components according to any one of claims 1 to 4, characterized in that the traditional Chinese medicine components are ultrafine powder.

8. The solid beverage of the probiotic compound composition containing traditional Chinese medicine components, as claimed in claim 7, wherein the grain size of the superfine powder is not more than 75 μm.

9. A preparation method of the solid beverage containing the probiotic compound composition containing the traditional Chinese medicine components is characterized by comprising the following steps:

step 2, auxiliary material pretreatment: drying inulin, fructo-oligosaccharide, blueberry fruit powder, skim milk powder and erythritol, and sieving with a 24-50-mesh sieve for later use;

step 5, weighing: weighing the inulin, the fructo-oligosaccharide, the blueberry fruit powder, the skim milk powder and the erythritol which are processed in the step 2 according to the mass parts, weighing the mulberry leaf powder or the yam powder and the coix seed powder which are processed in the step 4 according to the mass parts, and weighing the probiotic composition according to the mass parts for later use;

10. The solid beverage of the probiotic compound composition containing traditional Chinese medicine components according to the claims 1 to 4, characterized in that the dosage form of the solid beverage is granules, powder, and mixture of granules and powder.