CN114885964A - Application of beauveria bassiana and/or organic carbon fertilizer in apple disease control - Google Patents
Application of beauveria bassiana and/or organic carbon fertilizer in apple disease control Download PDFInfo
- Publication number
- CN114885964A CN114885964A CN202210486895.9A CN202210486895A CN114885964A CN 114885964 A CN114885964 A CN 114885964A CN 202210486895 A CN202210486895 A CN 202210486895A CN 114885964 A CN114885964 A CN 114885964A
- Authority
- CN
- China
- Prior art keywords
- organic carbon
- apple
- beauveria bassiana
- carbon fertilizer
- fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Soil Sciences (AREA)
- Mechanical Engineering (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the technical field of biological control of crop diseases, and discloses application of beauveria bassiana and/or organic carbon fertilizer in apple disease control. The biological organic carbon fertilizer provided by the invention is green and environment-friendly, can improve the soil quality, prevent and control microbial diversity imbalance and promote the formation of soil micro-ecological conditions for healthy growth of apples by applying the biological organic carbon fertilizer to soil, and has the characteristics of easily available raw materials, good control effect on apple fungal diseases and the like. The method can realize continuous and effective prevention and control of apple fungal diseases, and simultaneously improves the utilization rate of agricultural wastes, so that the economical efficiency of agricultural production and the income of farmers are improved.
Description
Technical Field
The invention relates to the technical field of biological control of crop diseases, in particular to application of beauveria bassiana and/or organic carbon fertilizer in apple disease control.
Background
Along with the structural adjustment of agricultural industry and the change of planting system and technology, the apple industry in China develops rapidly, and in recent years, the apple planting area in China is increased continuously, and the apple industry also develops rapidly. However, more problems still exist in the field of apple planting production, such as unstable apple yield and quality, and increasingly prominent apple disease problems, which seriously affect the continuous and healthy development of the industry.
The fungal diseases are one of the main diseases faced by apples, and the fungal diseases are not only harmful to the yield and quality of the apples in the same season or year, but also cause persistent damages such as successive reduction of yield, gradual decline of growth and even withering of the apples. In addition, pathogenic bacteria may also infect the soil of the planting field, thereby causing the yield and quality of subsequently planted apples and even apples in surrounding plantation to be reduced, and causing irreparable loss to fruit growers.
At present, the main treatment method for apple fungal diseases is to add chemical fertilizers and spray pesticides, however, excessive dependence on chemical fertilizers and chemical pesticides and the modern production mode of single crop continuous cropping can cause soil pollution and unbalanced distribution of soil nutrient elements, cause soil deterioration and unbalanced distribution of microbial communities, reduce the resistance of apple plants, further aggravate the occurrence of diseases, damage the environment, and be very unfavorable for green, healthy and sustainable development of the industry. Therefore, the research and development of effective apple disease control measures, especially long-term effective fungal disease control measures, have important significance for apple production in China and the world.
Disclosure of Invention
The invention aims to overcome the problems of lack of long-term continuous and effective measures for preventing and treating apple fungal diseases and the like in the prior art, and provides application of beauveria bassiana and/or organic carbon fertilizer in prevention and treatment of apple diseases. The invention takes the application of the beauveria bassiana and the biological organic carbon fertilizer as main control means, and has the advantages of economic and easily obtained raw materials, simple and easy control means, excellent control effect on various apple diseases and the like.
In order to achieve the above objects, one aspect of the present invention provides an application of Beauveria bassiana (Beauveria pseudobassiana) in the control of apple diseases.
The invention provides an organic carbon fertilizer, which comprises oil cake, mineral potassium fulvate, turf, traditional Chinese medicine residues and optional beauveria bassiana microbial inoculum;
wherein the weight ratio of oil cake, mineral potassium fulvate, turf and traditional Chinese medicine residue in the organic carbon fertilizer is 1:0.1-0.5:2-5: 1-4.
The third aspect of the invention provides application of the organic carbon fertilizer in apple disease control.
In a fourth aspect, the present invention provides a method for controlling apple diseases, comprising applying beauveria bassiana or the organic carbon fertilizer as described above to soil;
alternatively, the beauveria bassiana is contacted with the rhizosphere and/or root of the apple plant.
Through the technical scheme, the invention can obtain the following beneficial effects:
(1) according to the technical scheme provided by the invention, the beauveria bassiana and/or the biological organic carbon fertilizer are/is adopted to prevent and treat the apple diseases, so that a better continuous prevention and treatment effect can be obtained, and the problem that the prevention and treatment effect on the apple diseases, especially on the apple fungal diseases, is poor or the continuous prevention and treatment cannot be realized at present is effectively solved;
(2) the organic carbon fertilizer raw material provided by the invention adopts production byproducts (wastes) such as waste oil residues (oil slag) and waste traditional Chinese medicine residues of an oil refinery as raw materials, and has the characteristics of wide raw material source, low cost, environmental friendliness and simple preparation method;
(3) the method for preventing and treating apple diseases is simple and easy to implement, does not have adverse effect on environmental safety, and can promote green sustainable development of agricultural production;
(4) the organic carbon fertilizer provided by the invention adopts agricultural production wastes as main raw material sources, is low in cost, realizes agricultural waste recycling, can promote industrial structure adjustment, and has positive significance for improving the total agricultural yield and increasing the income of farmers.
Detailed Description
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and these ranges or values should be understood to encompass values close to these ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
The inventor of the invention skillfully discovers in the research process that when some beauveria bassiana (such as the beauveria bassiana CGMCC No.21047) is directly applied to soil or is in contact with the rhizosphere and/or the root of an apple plant (such as a beauveria bassiana culture is poured on the root of the apple plant), the better continuous control effect is achieved on apple diseases, particularly on apple fungal diseases.
Based on the above findings, the present invention provides, on the one hand, an application of Beauveria bassiana (Beauveria pseudobassiana) in apple disease control. The apple disease control can comprise disease prevention of an apple without diseases, and can also comprise corresponding disease treatment of an apple with diseases, so as to reduce the adverse effect of the diseases on the yield and/or quality of the apple.
According to a preferred embodiment of the present invention, wherein the application may comprise applying beauveria bassiana to the apple-planted soil. And/or contacting beauveria bassiana with rhizosphere and/or root of the apple plant. For example, Beauveria bassiana is cultured, and the obtained culture is poured into the rhizosphere and/or root of apple plants.
According to a preferred embodiment of the present invention, wherein the beauveria bassiana is beauveria bassiana with a collection number of CGMCC No. 21047.
According to a preferred embodiment of the present invention, wherein the disease is selected from apple fungal diseases, preferably apple rootstock diseases.
Preferably, the pathogen causing the apple disease comprises at least one of Fusarium oxysporum (Fusarium oxysporum), Fusarium solani (Fusarium solani) and Fusarium angulatum (Fusarium camptosceras), preferably Fusarium oxysporum.
The inventor of the invention also finds that the organic carbon fertilizer prepared by blending agricultural wastes such as oil cakes, turf and Chinese medicine residues according to a certain proportion and adding a proper amount of mineral potassium fulvate is applied to soil, can play a role in adjusting the abundance and community structure of microbial populations in soil, improving the proportion of beneficial bacteria in soil, reducing and eliminating the quantity and proportion of harmful bacteria, improving the soil quality, enhancing the immunocompetence of plants and improving the prevention and control effect on apple diseases. Through further research, after the beauveria bassiana is added into the organic carbon fertilizer, the prevention and control effect on apple diseases, particularly on apple fungal diseases, can be further improved.
The invention provides an organic carbon fertilizer, which comprises oil cake, mineral potassium fulvate, turf, traditional Chinese medicine residues and optional beauveria bassiana microbial inoculum;
wherein the weight ratio of oil cake, mineral potassium fulvate, grass carbon and traditional Chinese medicine residue in the organic carbon fertilizer is 1:0.1-0.5:2-5: 1-4.
Namely, the formula of the organic carbon fertilizer provided by the invention is any one of the following components:
(1) oil cake, mineral potassium fulvate, turf and traditional Chinese medicine residue in a weight ratio of 1:0.1-0.5:2-5:1-4 calculated on dry matter;
(2) oil cake, mineral potassium fulvate, turf, Chinese medicine dregs and beauveria bassiana microbial inoculum with the weight ratio of 1:0.1-0.5:2-5:1-4 calculated by dry matter.
The inventor of the invention also finds that when the adopted beauveria bassiana microbial inoculum contains beauveria bassiana capable of producing pig carriers, the control effect of apple diseases can be further improved, the growth of apples can be promoted, and the yield and the quality are improved.
In the present invention, beauveria bassiana capable of producing a pig carrier (preferably a hydroxamate type siderophore) is preferably used. According to a preferred embodiment of the present invention, the Beauveria bassiana is Beauveria bassiana with the preservation number of CGMCC No. 21047.
In the present invention, there is no particular limitation on the raw materials (such as oil cake, peat, herb residue, etc.) used as the organic carbon fertilizer, and it may be any related product that can be used in the art for preparing the organic carbon fertilizer, and it may be a commercially available related product or a related product that is prepared by itself according to the prior art.
According to a preferred embodiment of the present invention, wherein the oil cake has a moisture content of 15 to 25% by weight. Preferably 15-20 wt%.
Preferably, the oil cake has an organic carbon content of 45-65 wt%, a humic acid content of 10-20 wt% and a total nitrogen content of 2-10 wt% on a dry matter basis. Preferably the total phosphorus content of said oil cake (as P) 2 O 5 Calculated as K) is 1-10 wt%, and the total potassium content (calculated as K) 2 Calculated as O) is 1-5 wt%.
More preferablyIn the oil cake, the content of organic carbon is 55-65 wt%, the content of humic acid is 15-20 wt%, and the content of total nitrogen is 3-7 wt% on a dry matter basis. Preferably the total phosphorus content of said oil cake (as P) 2 O 5 Calculated as K) is 1-5 wt%, and the total potassium content (calculated as K) 2 Calculated as O) is 1-3 weight percent.
According to a preferred embodiment of the invention, the moisture content of the turf is not more than 20% by weight, preferably 10-15% by weight.
Preferably, the turf contains 40-50 wt% of organic carbon, 20-35 wt% of humic acid and 1-2 wt% of total nitrogen on a dry matter basis. Preferably the total phosphorus content (in P) of the grass carbon 2 O 5 Calculated as K) is 0.1-1 wt%, and the total potassium content is calculated as K 2 Calculated as O) is 1-5 wt%.
More preferably, the turf has an organic carbon content of 40-45 wt%, a humic acid content of 20-30 wt% and a total nitrogen content of 1.5-2 wt% on a dry matter basis. Preferably the total phosphorus content (in P) of the grass carbon 2 O 5 Calculated as K) is 0.1-0.5 wt%, and the total potassium content (calculated as K) 2 Calculated as O) is 1-2 weight percent.
According to a preferred embodiment of the present invention, the moisture content of the herb residue is 20 to 30 wt%. Preferably 20 to 25 wt%.
Preferably, the traditional Chinese medicine residues contain 30-60 wt% of organic carbon, 15-25 wt% of humic acid and 2-3 wt% of total nitrogen on a dry matter basis. Preferably, the total phosphorus content (as P) in the herb residue 2 O 5 Calculated as K) is 0.1-1 wt%, and the total potassium content (calculated as K) 2 Calculated as O) is 1-2 weight percent.
More preferably, the traditional Chinese medicine residue contains 50-60 wt% of organic carbon, 20-25 wt% of humic acid and 2-2.5 wt% of total nitrogen on a dry matter basis. Preferably, the total phosphorus content (as P) in the herb residue 2 O 5 Calculated as K) is 0.5-1 wt%, and the total potassium content (calculated as K) 2 Calculated as O) is 1 to 1.5 weight percent.
Preferably, the mineral source potassium fulvate contains 40-60 wt% fulvic acid on a dry matter basisThe content of reproductive acid is 25-50 wt%, and the content of total potassium (expressed as K) 2 Calculated by O) is 10-15 wt%.
More preferably, the mineral source potassium fulvate contains 45-55 wt% of fulvic acid, 30-40 wt% of humic acid and total potassium (in terms of K) on a dry matter basis 2 Calculated as O) is 12-14 wt%.
According to a preferred embodiment of the present invention, the organic carbon fertilizer further comprises an auxiliary material. Preferably, the total content of the auxiliary materials is 1-5 wt%, preferably 1-3 wt%, based on the total weight of the organic carbon fertilizer.
Preferably, the auxiliary material is selected from at least one of a nitrogen fertilizer, a phosphate fertilizer and a potassium fertilizer.
In the present invention, there is no particular limitation on the nitrogenous fertilizer, phosphate fertilizer and potash fertilizer selected, and they may be organic fertilizers or inorganic fertilizers as long as they can provide N, P and K. It can be either a related product obtained commercially or a related product formulated or prepared by itself according to the prior art.
Preferably, the beauveria bassiana microbial inoculum is used in an amount of 0.1-2 wt% based on the total weight of the organic carbon fertilizer, and preferably, the beauveria bassiana microbial inoculum is used in an amount such that the content of beauveria bassiana in the organic carbon fertilizer is 1 x 10 9 -5×10 9 CFU/g. Preferably 1X 10 9 -3×10 9 CFU/g. More preferably 1X 10 9 -2×10 9 CFU/g。
The third aspect of the invention provides the application of the organic carbon fertilizer in apple disease control.
According to a preferred embodiment of the present invention, the apple disease is apple fungal disease, preferably apple rootstalk disease.
Preferably, the pathogen causing the disease comprises at least one of fusarium oxysporum, fusarium solani and fusarium angustifolium, preferably fusarium oxysporum.
In a fourth aspect, the present invention provides a method for controlling apple diseases, which comprises applying beauveria bassiana or the organic carbon fertilizer as described above to soil;
alternatively, the beauveria bassiana is contacted with the rhizosphere and/or root of the apple plant.
That is, the above method may include the following modes:
(1) applying beauveria bassiana (microbial inoculum) to soil;
(2) applying an organic carbon fertilizer to soil;
(3) beauveria bassiana (microbial inoculum) is contacted with rhizosphere and/or root of an apple plant.
According to a preferred embodiment of the present invention, the apple disease is apple fungal disease, preferably apple rootstalk disease.
Preferably, the pathogen causing the apple disease comprises at least one of fusarium oxysporum, fusarium solani and fusarium angustifolium, preferably fusarium oxysporum.
According to a preferred embodiment of the present invention, wherein the beauveria bassiana is selected from beauveria bassiana capable of producing (hydroxamate type) siderophores, preferably the beauveria bassiana with the collection number of CGMCC No. 21047.
According to a preferred embodiment of the present invention, wherein, in the above aspect (1), the beauveria bassiana is used in an amount of 3 × 10 12 -3×10 13 CFU/strain. Preferably 3X 10 12 -1.5×10 13 CFU/strain. More preferably 6X 10 12 -1.2×10 13 CFU/strain. More preferably 9X 10 12 -1.2×10 13 CFU/strain.
According to the preferable embodiment of the present invention, wherein, in the above aspect (2), the organic carbon fertilizer is used in an amount of 15000-30000 kg-hm -2 。
According to a preferred embodiment of the present invention, wherein, in the above aspect (3), the beauveria bassiana is used in an amount of 3X 10 12 -3×10 13 CFU/strain. Preferably 3X 10 12 -1.5×10 13 CFU/strain. More preferably 6X 10 12 -1.2×10 13 CFU/strain. More preferably 9X 10 12 -1.2×10 13 CFU/strain.
In the present invention, with respect to the above modes (1) and (3), the application form (microbial agent dosage form) of the beauveria bassiana (microbial agent) is not particularly limited, and can be selected by those skilled in the art according to actual circumstances. For example, the beauveria bassiana solid microbial inoculum can be directly applied to soil, or dissolved in water and applied to the soil, or a culture medium suitable for beauveria bassiana is adopted to culture the beauveria bassiana, and the obtained culture solution is poured into the soil or poured into the rhizosphere and/or the root of an apple plant.
Preferably, the application frequency of the beauveria bassiana or the organic carbon fertilizer is 2-3 times per crop.
Preferably, the method further comprises composting the organic carbon fertilizer prior to application, preferably the composting conditions comprise: humidity of 40-45%, temperature of 60-75 deg.C, and time of 30-50 days. In order to obtain better composting effect, the pile can be turned over in the composting process. Preferably, the compost can be turned for 1 time every 10 to 20 days in the composting process.
The present invention will be described in detail below by way of examples. It should be understood that the following examples are only intended to further illustrate and explain the present invention, and are not intended to limit the present invention.
In the following examples, mineral potassium fulvate was obtained from naproxen agricultural science and technology ltd, oil cake was collected from waste oil cake (rapeseed oil cake) produced in Kunming xi oil mill, peat was prepared from peat soil and humus soil (weight ratio 6:4), and Chinese medicinal residue was obtained from waste Chinese medicinal residue (35 wt% of Panax notoginseng, 30 wt% of Codonopsis pilosula, 25 wt% of Paris polyphylla, 10 wt% of Pueraria lobata) produced in Kunming Chinese medicinal preparation and/or Chinese medicinal preparation production by heap rotting fermentation. Urea, calcium superphosphate, potassium sulfate, and the like are commercially available products from regular chemical company. Wherein the mass fraction of N in the urea is 46 percent, and P in the calcium superphosphate 2 O 5 16 percent of potassium sulfate and K in the potassium sulfate 2 The mass fraction of O is 50%. The Beauveria bassiana (CGMCC No.21047, its acquisition mode can refer to CN202011398784.X) microbial inoculum is a solid microbial inoculum prepared by expanding culture and drying the strain, wherein the Beauveria bassiana is about 3 × 10 11 CFU/g. The content of organic carbon in tea residue, mesochrome and turf is determined by adopting a potassium dichromate external heating method, the content of total nitrogen is determined by adopting a Kjeldahl method, phosphorus is determined by adopting a phosphomolybdic acid quinoline gravimetric method, potassium is determined by adopting a potassium tetraphenylborate gravimetric method, humic acid is determined by adopting a sodium pyrophosphate alkali liquor extraction method, the content of fulvic acid in mineral source fulvic acid is obtained by calculating the content of other substances, and the pH is determined by adopting a potential method, wherein the main components are shown in Table 1 (wherein the water content is calculated by taking the total weight of materials as a reference, and the other components are calculated by taking dry substances in the materials as a reference).
TABLE 1 organic carbon fertilizer Material composition
Example 1
100kg of mineral potassium fulvate, 100kg of oil cake, 300kg of grass carbon and 300kg of traditional Chinese medicine residues are taken, and then the materials are mixed, piled and tightly sealed by mud after being piled. During the composting period, the water content in the compost material is controlled to be 45 +/-5 percent, and the temperature is controlled to be 65 +/-5 ℃. Turning the compost for 1 time on the 15 th day and the 30 th day respectively. And composting for 40 days to obtain organic carbon fertilizer A1.
Example 2
100kg of mineral potassium fulvate, 80kg of oil cake, 420kg of turf and 200kg of traditional Chinese medicine residues are taken, and then the materials are mixed and piled up, and the piled materials are covered by plastic cloth. During the composting period, the water content in the compost materials is controlled to be 45 +/-5 percent, the temperature is controlled to be 65 +/-5 ℃, and the compost is turned for 1 time on the 15 th day and the 30 th day respectively. And composting for 35 days to obtain organic carbon fertilizer A2.
Example 3
100kg of mineral potassium fulvate, 50kg of oil cake, 500kg of turf and 150kg of traditional Chinese medicine residue are taken, and then the materials are mixed and piled up, and the piled materials are tightly sealed by mud. During the composting period, the water content in the compost materials is controlled to be 45 +/-5 percent, the temperature is controlled to be 65 +/-5 ℃, the compost is turned over for 1 time on the 15 th day, and the composting lasts for 30 days. Obtaining the organic carbon fertilizer A3.
Example 4
The organic carbon fertilizer a1 in example 1 was mixed with a fertilizer additive in an amount of 1 wt% based on the total weight of the organic carbon fertilizer to obtain an organic carbon fertilizer B1. The fertilizer additive is prepared by urea, calcium superphosphate and potassium sulfate, wherein the weight ratio of N, P, K is 8:16: 26.
Example 5
The organic carbon fertilizer a1 in example 1 was mixed with a beauveria bassiana microbial inoculum in an amount of 0.5 wt% based on the total weight of the organic carbon fertilizer to obtain an organic carbon fertilizer B2.
Example 6
The procedure of example 1 was followed except that the mineral source potassium fulvate was replaced with an equal weight of the major ingredient (on a dry basis) of the biochemical potassium fulvate shown in table 2. Obtaining the organic carbon fertilizer A4.
TABLE 2 replacement of Biochemical Potassium Fulvate composition
Comparative example 1
The organic carbon fertilizer a1 in example 1 was mixed with a Beauveria bassiana (Beauveria bassiana, available from behaveria boviet biotechnology limited, beijing, strain number bio-19292, which was determined to produce no iron carrier) microbial inoculum in an amount of 0.5 wt% based on the total weight of the organic carbon fertilizer, to obtain an organic carbon fertilizer B3.
Comparative example 2
The method of example 1 was followed except that oil cake and peat therein were replaced with equal weight of herb residue. Obtaining the organic carbon fertilizer D1.
Comparative example 3
The method of example 1 was followed except that the peat was replaced with equal weight of herb residue. Obtaining the organic carbon fertilizer D2.
Comparative example 4
The method of example 1 was followed except that herb residue and oil cake therein were replaced with peat of equal weight. Obtaining the organic carbon fertilizer D3.
Comparative example 5
The procedure of example 1 was followed except that the peat contained in the same was replaced with equal amounts by weight of tea leaves and oil cakes (the weight ratio of tea leaves to oil cakes was the same as in example 1) in accordance with the procedure of example 1. Obtaining the organic carbon fertilizer D4.
Comparative example 6
The procedure of example 1 was followed except that 250kg of humus soil was used in place of the peat (the amount of organic carbon supplied from the humus soil was substantially the same as that supplied from the peat in example 1). Obtaining the organic carbon fertilizer D5.
Comparative example 7
According to the method of the embodiment 4, except that the organic carbon fertilizer D1 is used for replacing the organic carbon fertilizer A1, the organic carbon fertilizer B4 is obtained.
Comparative example 8
According to the method of the embodiment 4, except that the organic carbon fertilizer D2 is used for replacing the organic carbon fertilizer A1, the organic carbon fertilizer B5 is obtained.
Comparative example 9
According to the method of the embodiment 5, except that the organic carbon fertilizer D3 is adopted to replace the organic carbon fertilizer A1, the organic carbon fertilizer B6 is obtained.
Test example 1
The organic carbon fertilizers in the above examples and comparative examples were applied to the soil once after harvesting apples in autumn and once in the germination period in spring, respectively, in apple orchards affected by different fungi and causing apple root rot according to the amounts shown in table 3.
Treatment groups (application of organic carbon fertilizer according to the test conditions of table 3) and control groups (no application of organic carbon fertilizer) were divided in different areas in the orchard, 10 apple trees were treated in each test group, the incidence of disease was investigated at harvest time according to the following criteria, and the control effect was calculated.
Apple root rot investigation grading standard:
level 0: root and stem base normal;
level 1: the roots and the stem bases have a few disease spots;
and 3, stage: the number of diseased spots at the root and the stem base is large, and the area is 25 to 50 percent of the total area of the root or the stem base;
and 5, stage: the scab at the root and the base of the stem is larger, and the area is 50 to 75 percent of the total area of the root or the base of the stem;
and 7, stage: the root and the base of the stem are connected with the scab to form a root-stem winding phenomenon, but the root system does not die;
and 9, stage: root necrosis, wilting or death of the overground part of the plant.
The prevention and treatment effect is calculated by adopting the following two formulas:
disease index [ (disease-grade plant number × representative grade))/(total plant number × highest representative grade) ] × 100
The preventing and treating effect (%) is [ (disease index of control group-disease index of treatment group)/disease index of control group ] × 100%
TABLE 3 biological organic carbon fertilizer for apple fungus disease control effect test conditions and results
Test example 2
Culturing Beauveria bassiana CGMCC No.21047 with potato glucose culture medium (purchased from Qingdao high-tech industrial Yuanbo biotechnology Co., Ltd., main components of 6% potato extract powder and 20% glucose) to obtain viable count of about 3 × 10 11 CFU/mL beauveria bassiana culture solution.
Apples were treated in the following manner, each treatment comprising 10 apples:
experimental group I: the beauveria bassiana culture solution is poured into the rhizosphere soil of the apples and is respectively applied once after the apples are harvested in autumn and at the germination stage in spring.
Experimental group II: the preparation method comprises mixing Beauveria bassiana (solid microbial inoculum with viable count of about 3 × 10) 11 CFU/g) is applied to rhizosphere soil where apples are planted, and the CFU/g) is respectively applied once after the apples are harvested in autumn and once in the germination period in spring.
Control group: the beauveria bassiana culture solution and the beauveria bassiana microbial inoculum are not applied.
According to the calculation method in the test example 1, the control effects of apple diseases of each group are calculated and compared, and the results are detailed in table 4.
TABLE 4 Beauveria bassiana (balsamo) Vuillemin test conditions and results for apple fungal disease control effect test
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. Application of Beauveria bassiana (Beauveria pseudobassiana) in preventing and treating apple diseases.
2. The use according to claim 1, wherein the beauveria bassiana is beauveria bassiana with a collection number of CGMCC No. 21047;
and/or the disease is selected from apple fungal diseases, preferably apple rootstock diseases;
preferably, the pathogen causing the apple disease includes at least one of Fusarium oxysporum (Fusarium oxysporum), Fusarium solani (Fusarium solani) and Fusarium angustifolium (Fusarium camptoseras), preferably Fusarium oxysporum.
3. The organic carbon fertilizer is characterized by comprising oil cakes, mineral potassium fulvate, turf, traditional Chinese medicine residues and optional beauveria bassiana microbial inoculum;
wherein the weight ratio of oil cake, mineral potassium fulvate, turf and traditional Chinese medicine residue in the organic carbon fertilizer is 1:0.1-0.5:2-5: 1-4.
4. The organic carbon fertilizer according to claim 3, wherein the beauveria bassiana is a beauveria bassiana with a preservation number of CGMCC No. 21047;
and/or the water content in the oil cake is 15-25 wt%;
and/or, the water content in the turf is not more than 20 wt%, preferably 10-15 wt%;
and/or, the water content in the traditional Chinese medicine residue is 20-30 wt%;
preferably, the oil cake contains 45-65 wt% of organic carbon, 10-20 wt% of humic acid and 2-10 wt% of total nitrogen on a dry matter basis;
preferably, in the mineral source potassium fulvate, the content of fulvic acid is 40-60 wt%, the content of humic acid is 25-50 wt%, and the content of total potassium is 10-15 wt% on a dry matter basis;
preferably, the turf contains 40-50 wt% of organic carbon, 20-35 wt% of humic acid and 1-2 wt% of total nitrogen on a dry matter basis;
preferably, the traditional Chinese medicine residues contain 30-60 wt% of organic carbon, 15-25 wt% of humic acid and 2-3 wt% of total nitrogen on a dry matter basis.
5. The organic carbon fertilizer according to claim 3, wherein the organic carbon fertilizer further comprises auxiliary materials, preferably the total content of the auxiliary materials is 1-5 wt%, preferably 1-3 wt%;
preferably, the beauveria bassiana microbial inoculum is used in an amount of 0.1-2 wt% based on the total weight of the organic carbon fertilizer, and preferably, the beauveria bassiana microbial inoculum is used in an amount such that the content of beauveria bassiana in the organic carbon fertilizer is 1 x 10 9 -5×10 9 CFU/g；
Preferably, the auxiliary material is selected from at least one of a nitrogen fertilizer, a phosphate fertilizer and a potassium fertilizer.
6. Use of the organic carbon fertilizer of any one of claims 3-5 for the control of apple diseases.
7. Use according to claim 6, wherein the apple diseases are apple fungal diseases, preferably apple rootstock diseases;
preferably, the pathogen causing the disease comprises at least one of fusarium oxysporum, fusarium solani and fusarium angustifolium, preferably fusarium oxysporum.
8. A method for controlling apple diseases, which comprises applying beauveria bassiana or the organic carbon fertilizer of any one of claims 3 to 5 to soil;
alternatively, the beauveria bassiana is contacted with the rhizosphere and/or root of the apple plant.
9. The method according to claim 8, wherein the apple disease is an apple fungal disease, preferably an apple rootstock disease;
and/or the beauveria bassiana is selected from beauveria bassiana with the preservation number of CGMCC No. 21047;
preferably, the pathogen causing the disease comprises at least one of fusarium oxysporum, fusarium solani and fusarium angustifolium, preferably fusarium oxysporum.
10. The method of claim 8, wherein the beauveria bassiana is administered in an amount of 3 x 10 12 -3×10 13 CFU/strain;
and/or the use amount of the organic carbon fertilizer is 15000-30000 kg-hm -2 ；
Preferably, the application frequency of the beauveria bassiana or the organic carbon fertilizer is 2-3 times per crop;
preferably, the method further comprises composting the organic carbon fertilizer prior to application, preferably the composting conditions comprise: humidity of 40-45%, temperature of 60-75 deg.C, and time of 30-50 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210486895.9A CN114885964B (en) | 2022-05-06 | 2022-05-06 | Application of beauveria bassiana and/or organic carbon fertilizer in apple disease control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210486895.9A CN114885964B (en) | 2022-05-06 | 2022-05-06 | Application of beauveria bassiana and/or organic carbon fertilizer in apple disease control |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114885964A true CN114885964A (en) | 2022-08-12 |
| CN114885964B CN114885964B (en) | 2023-05-12 |
Family
ID=82719586
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210486895.9A Active CN114885964B (en) | 2022-05-06 | 2022-05-06 | Application of beauveria bassiana and/or organic carbon fertilizer in apple disease control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114885964B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116120127A (en) * | 2022-11-30 | 2023-05-16 | 云南省农业科学院农业环境资源研究所 | Biological organic fertilizer and application thereof |
| CN116253600A (en) * | 2022-11-30 | 2023-06-13 | 云南省农业科学院农业环境资源研究所 | Organic fertilizer and method for promoting vegetable growth and/or preventing and controlling vegetable diseases |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160075992A1 (en) * | 2014-09-15 | 2016-03-17 | The United States Of America, As Represented By The Secretary Of Agriculture | Stable Fungal Blastospores and Methods for Their Production, Stabilization and Use |
| US20180325120A1 (en) * | 2017-05-10 | 2018-11-15 | The United States Of America, As Represented By The Secretary Of Agriculture | Compositions and methods to reduce the population of wheat-stem sawfly and hessian fly |
| CN110483111A (en) * | 2019-09-12 | 2019-11-22 | 广西垂青生物科技有限公司 | A kind of biological organic fertilizer and preparation method and application for preventing and treating soil-borne disease |
| KR102121945B1 (en) * | 2018-12-04 | 2020-06-11 | 대한민국 | Entomopathogenic fungi and insecticide comprising same |
| CN111848242A (en) * | 2020-07-29 | 2020-10-30 | 福建三炬生物科技股份有限公司 | Compound microbial agent with biocontrol function and preparation method and application thereof |
| CN112136836A (en) * | 2020-09-17 | 2020-12-29 | 云南省农业科学院农业环境资源研究所 | Application of organic carbon fertilizer in preventing and treating crop soil-borne diseases and method for preventing and treating crop soil-borne diseases |
| CN112194532A (en) * | 2020-09-17 | 2021-01-08 | 云南省烟草公司保山市公司 | Organic carbon fertilizer and application thereof and method for preventing and treating tobacco rhizome diseases |
| CN112501031A (en) * | 2020-12-01 | 2021-03-16 | 云南省农业科学院农业环境资源研究所 | Beauveria bassiana (balsamo) Vuillemin and application thereof |
-
2022
- 2022-05-06 CN CN202210486895.9A patent/CN114885964B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160075992A1 (en) * | 2014-09-15 | 2016-03-17 | The United States Of America, As Represented By The Secretary Of Agriculture | Stable Fungal Blastospores and Methods for Their Production, Stabilization and Use |
| US20180325120A1 (en) * | 2017-05-10 | 2018-11-15 | The United States Of America, As Represented By The Secretary Of Agriculture | Compositions and methods to reduce the population of wheat-stem sawfly and hessian fly |
| KR102121945B1 (en) * | 2018-12-04 | 2020-06-11 | 대한민국 | Entomopathogenic fungi and insecticide comprising same |
| CN110483111A (en) * | 2019-09-12 | 2019-11-22 | 广西垂青生物科技有限公司 | A kind of biological organic fertilizer and preparation method and application for preventing and treating soil-borne disease |
| CN111848242A (en) * | 2020-07-29 | 2020-10-30 | 福建三炬生物科技股份有限公司 | Compound microbial agent with biocontrol function and preparation method and application thereof |
| CN112136836A (en) * | 2020-09-17 | 2020-12-29 | 云南省农业科学院农业环境资源研究所 | Application of organic carbon fertilizer in preventing and treating crop soil-borne diseases and method for preventing and treating crop soil-borne diseases |
| CN112194532A (en) * | 2020-09-17 | 2021-01-08 | 云南省烟草公司保山市公司 | Organic carbon fertilizer and application thereof and method for preventing and treating tobacco rhizome diseases |
| CN112501031A (en) * | 2020-12-01 | 2021-03-16 | 云南省农业科学院农业环境资源研究所 | Beauveria bassiana (balsamo) Vuillemin and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116120127A (en) * | 2022-11-30 | 2023-05-16 | 云南省农业科学院农业环境资源研究所 | Biological organic fertilizer and application thereof |
| CN116253600A (en) * | 2022-11-30 | 2023-06-13 | 云南省农业科学院农业环境资源研究所 | Organic fertilizer and method for promoting vegetable growth and/or preventing and controlling vegetable diseases |
| CN116120127B (en) * | 2022-11-30 | 2024-02-02 | 云南省农业科学院农业环境资源研究所 | Biological organic fertilizer and application thereof |
| CN116253600B (en) * | 2022-11-30 | 2024-02-02 | 云南省农业科学院农业环境资源研究所 | Organic fertilizer and method for promoting vegetable growth and/or preventing and controlling vegetable diseases |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114885964B (en) | 2023-05-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103113144B (en) | Organic fertilizer for tobacco and preparation method thereof | |
| CN101165008B (en) | Vegetable residual strain microorganism-earthworm multiple step inoculation conversion method | |
| WO2021169152A1 (en) | Method for preparing carbon-based fertilizer using perishable waste as raw material, and application | |
| CN103992184A (en) | Bio-organic fertilizer | |
| CN112573974A (en) | Biological salt-decomposing bacterial fertilizer and preparation method thereof | |
| CN114885964A (en) | Application of beauveria bassiana and/or organic carbon fertilizer in apple disease control | |
| CN115044481B (en) | Application of trichoderma and/or organic carbon fertilizer in tsaoko disease control | |
| CN116253600B (en) | Organic fertilizer and method for promoting vegetable growth and/or preventing and controlling vegetable diseases | |
| CN109536410B (en) | Salt-tolerant growth-promoting composite microbial inoculum and preparation method and application thereof | |
| CN112194532B (en) | Organic carbon fertilizer and application thereof and method for preventing and treating tobacco rhizome diseases | |
| CN107338056B (en) | Green microbial soil conditioner and preparation method thereof | |
| CN113812327B (en) | Vegetable seedling substrate based on waste utilization and preparation method thereof | |
| CN103771924A (en) | Special flower planting organic fertilizer and preparation method thereof | |
| CN104945137A (en) | Enzyme mineral organic fertilizer and method for preparing the same | |
| CN106348865A (en) | Organic-inorganic compound fertilizer with silkworm excrement and tobacco stems as organic raw materials and preparation method of organic-inorganic compound fertilizer | |
| CN107540461B (en) | Ecological organic fertilizer with soil improvement function and production method thereof | |
| CN104844285A (en) | Method for preparing bio-organic fertilizer for improving immunity of cherry tomatoes | |
| CN107176855B (en) | Bio-organic fertilizer and application thereof | |
| CN111072420A (en) | Compound amino acid microbial agent and preparation method thereof | |
| CN106278403A (en) | A kind of agriculture microorganism formulation for soil in protected field conditioning and preparation method thereof | |
| CN105542784A (en) | Soil conditioner suitable for cornflower growth and preparation method thereof | |
| KR20110077703A (en) | Compost comprising cocopeat and peat, and the preparation method thereof | |
| CN114149808A (en) | Soil conditioner for preventing and treating tobacco bacterial wilt and improving proportion of middle-grade and high-grade tobacco leaves and preparation method thereof | |
| CN108610177B (en) | Trichoderma whole-element biological organic fertilizer special for banana crops and preparation method and application thereof | |
| CN111517877A (en) | Special biological source bacterial fertilizer system for cherries and application method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |