CN114874349B - 一种基于场流分离技术分离纯化灵芝多糖的方法 - Google Patents
一种基于场流分离技术分离纯化灵芝多糖的方法 Download PDFInfo
- Publication number
- CN114874349B CN114874349B CN202210675812.0A CN202210675812A CN114874349B CN 114874349 B CN114874349 B CN 114874349B CN 202210675812 A CN202210675812 A CN 202210675812A CN 114874349 B CN114874349 B CN 114874349B
- Authority
- CN
- China
- Prior art keywords
- ultrafiltration
- field flow
- polysaccharide
- flow separation
- separating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 55
- 150000004676 glycans Chemical class 0.000 claims abstract description 69
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 65
- 239000005017 polysaccharide Substances 0.000 claims abstract description 65
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 58
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 42
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 42
- 239000012528 membrane Substances 0.000 claims abstract description 39
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 30
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 30
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 27
- 239000012498 ultrapure water Substances 0.000 claims abstract description 27
- 229920002678 cellulose Polymers 0.000 claims abstract description 17
- 239000001913 cellulose Substances 0.000 claims abstract description 17
- 239000000243 solution Substances 0.000 claims description 20
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 14
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 14
- 238000002390 rotary evaporation Methods 0.000 claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 241000222336 Ganoderma Species 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 230000010355 oscillation Effects 0.000 claims description 7
- 239000002244 precipitate Substances 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 238000004108 freeze drying Methods 0.000 claims description 4
- 150000004804 polysaccharides Polymers 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000005303 weighing Methods 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims description 2
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims 1
- 238000000746 purification Methods 0.000 abstract description 13
- 238000000502 dialysis Methods 0.000 abstract description 6
- 238000010612 desalination reaction Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 description 36
- 235000018102 proteins Nutrition 0.000 description 27
- 239000000523 sample Substances 0.000 description 24
- 230000007423 decrease Effects 0.000 description 5
- 230000036571 hydration Effects 0.000 description 5
- 238000006703 hydration reaction Methods 0.000 description 5
- 238000010828 elution Methods 0.000 description 4
- 238000001228 spectrum Methods 0.000 description 4
- 238000002211 ultraviolet spectrum Methods 0.000 description 4
- 229920012266 Poly(ether sulfone) PES Polymers 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000011033 desalting Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 235000004252 protein component Nutrition 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 241001489091 Ganoderma sinense Species 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 238000011034 membrane dialysis Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000012521 purified sample Substances 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000005556 structure-activity relationship Methods 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/10—Process efficiency
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
Description
Claims (4)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210675812.0A CN114874349B (zh) | 2022-06-15 | 2022-06-15 | 一种基于场流分离技术分离纯化灵芝多糖的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210675812.0A CN114874349B (zh) | 2022-06-15 | 2022-06-15 | 一种基于场流分离技术分离纯化灵芝多糖的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN114874349A CN114874349A (zh) | 2022-08-09 |
CN114874349B true CN114874349B (zh) | 2023-03-10 |
Family
ID=82681579
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210675812.0A Active CN114874349B (zh) | 2022-06-15 | 2022-06-15 | 一种基于场流分离技术分离纯化灵芝多糖的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114874349B (zh) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016108365A (ja) * | 2014-12-02 | 2016-06-20 | 花王株式会社 | バイオマスの成分分離方法 |
CN106146684A (zh) * | 2016-07-26 | 2016-11-23 | 海安县泓寿生物技术有限责任公司 | 破壁灵芝孢子粉灵芝多糖的分离纯化方法 |
CN112940145A (zh) * | 2021-04-07 | 2021-06-11 | 江西嘉博生物工程有限公司 | 一种动物用灵芝多糖的提取制备方法 |
CN114605566A (zh) * | 2022-03-14 | 2022-06-10 | 中国科学院长春应用化学研究所 | 一种提取芦荟凝胶大分子活性成分的方法及其应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3775926A1 (en) * | 2018-08-30 | 2021-02-17 | Regeneron Pharmaceuticals, Inc. | Methods for characterizing protein complexes |
CN109991067A (zh) * | 2019-04-30 | 2019-07-09 | 河北大学 | 一种准确检测天麻多糖回转半径及分子量分布的方法 |
-
2022
- 2022-06-15 CN CN202210675812.0A patent/CN114874349B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2016108365A (ja) * | 2014-12-02 | 2016-06-20 | 花王株式会社 | バイオマスの成分分離方法 |
CN106146684A (zh) * | 2016-07-26 | 2016-11-23 | 海安县泓寿生物技术有限责任公司 | 破壁灵芝孢子粉灵芝多糖的分离纯化方法 |
CN112940145A (zh) * | 2021-04-07 | 2021-06-11 | 江西嘉博生物工程有限公司 | 一种动物用灵芝多糖的提取制备方法 |
CN114605566A (zh) * | 2022-03-14 | 2022-06-10 | 中国科学院长春应用化学研究所 | 一种提取芦荟凝胶大分子活性成分的方法及其应用 |
Non-Patent Citations (3)
Title |
---|
Applications of asymmetrical flow field-flow fractionation for separation and characterization of polysaccharides: A review;Xue Chena et al.;《Journal of chromatography. A》;20201119;第1635卷;第1节、第3.3节第4.3.2节 * |
Asymmetrical flow field-flow fractionation combined with ultrafiltration: A novel and high-efficiency approach for separation, purification, and characterization of Ganoderma lucidum polysaccharides;Yuxi Guo et al.;《Talanta》;20221023;第1-7页 * |
基于非对称流场流分离技术的蛋白分离研究;鄂云龙等;《北京化工大学学报(自然科学版)》;20150320(第02期);第1.3节和第3节 * |
Also Published As
Publication number | Publication date |
---|---|
CN114874349A (zh) | 2022-08-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111440218A (zh) | 一种植物多酚的制备方法 | |
KR20120108816A (ko) | 천연 휴믹 물질로 부터 휴믹산과 풀빅산 추출방법 | |
CN1680359A (zh) | 膜超滤法提纯葡萄籽中的低聚原花青素的方法 | |
CN101455287A (zh) | 蜂毒肽的提纯方法 | |
CN101089017A (zh) | 蜂毒肽的分离提纯方法 | |
CN114874349B (zh) | 一种基于场流分离技术分离纯化灵芝多糖的方法 | |
CN107698691B (zh) | 一种从白花蛇舌草中分离纯化黄酮、多糖的***及方法 | |
CN103830298A (zh) | 一种膜联用技术制备三七总皂苷的方法 | |
CN102784193A (zh) | 采用耦合技术制备红芪提取物的方法 | |
CN110256597B (zh) | 一种膜法降低灵芝多糖中重金属残留的方法 | |
CN108118044A (zh) | 一种分离提纯蛋清溶菌酶的方法 | |
CN1823908A (zh) | 应用膜分离技术对夏桑菊提取液精制的新工艺 | |
CN1240723C (zh) | 玉米浸泡水中提取、提纯脂多糖的方法 | |
CN113549162A (zh) | 一种柘木精制多糖的制备方法 | |
CN113603805B (zh) | 一种白刺多糖的制备方法 | |
CN113440547B (zh) | 采用大孔树脂串联动态轴向压缩柱分离纯化大蓟总苷的方法 | |
CN1401660A (zh) | 一种微囊藻毒素的提取纯化方法 | |
CN105037451B (zh) | 一种超滤处理黄霉素发酵液的方法 | |
CN101073666A (zh) | 一种制备高纯度胰激肽原酶原料药的方法 | |
CN102584611A (zh) | 一种药用级缬氨酸的生产方法 | |
CN105753974A (zh) | 一种基于亲和层析柱的乌司他丁纯化方法 | |
CN105837685A (zh) | 一种基于阴离子交换树脂纯化乌司他丁的方法 | |
CN104017037B (zh) | 一种井冈霉素原粉的制备方法 | |
CN105801723B (zh) | 一种海洋硫酸多糖的快速纯化方法 | |
CN116396408B (zh) | 一种番石榴多糖制备方法、多糖及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20220809 Assignee: Hebei Guangxun Biotechnology Co.,Ltd. Assignor: HEBEI University Contract record no.: X2024990000042 Denomination of invention: A method for separating and purifying Ganoderma lucidum polysaccharides based on field flow separation technology Granted publication date: 20230310 License type: Common License Record date: 20240117 Application publication date: 20220809 Assignee: Hanpai Traditional Chinese Medicine Hebei Co.,Ltd. Assignor: HEBEI University Contract record no.: X2024990000039 Denomination of invention: A method for separating and purifying Ganoderma lucidum polysaccharides based on field flow separation technology Granted publication date: 20230310 License type: Common License Record date: 20240117 Application publication date: 20220809 Assignee: Hangzhou Yiju Juqi Biotechnology Co.,Ltd. Assignor: HEBEI University Contract record no.: X2024990000038 Denomination of invention: A method for separating and purifying Ganoderma lucidum polysaccharides based on field flow separation technology Granted publication date: 20230310 License type: Common License Record date: 20240117 |