CN114853858B - 一种抗虫性的苏铁毒蛋白基因、表达载体及应用 - Google Patents

一种抗虫性的苏铁毒蛋白基因、表达载体及应用 Download PDF

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CN114853858B
CN114853858B CN202210324163.XA CN202210324163A CN114853858B CN 114853858 B CN114853858 B CN 114853858B CN 202210324163 A CN202210324163 A CN 202210324163A CN 114853858 B CN114853858 B CN 114853858B
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邹长松
王朋宝
张寿洲
黄锦岭
马建超
刘欢
常贯晓
李林洲
王思博
董珊珊
程祥飞
王磊
孙晓
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Shenzhen Xianhu Botanical Garden Shenzhen Garden Research Center
BGI Shenzhen Co Ltd
Henan University
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Abstract

本发明属于基因工程技术领域，公开了一种抗虫性的苏铁毒蛋白基因、表达载体及应用，所述抗虫性的苏铁毒蛋白基因及其同源基因的核苷酸序列为SEQ ID NO.1，SEQ ID NO.2，SEQ ID NO.3，SEQ ID NO.4，SEQ ID NO.5。本发明提供的毒蛋白基因来自苏铁，外源表达注射发现其对小菜蛾与棉铃虫幼虫具有毒性作用。将其导入到棉花中，使棉花获得草地贪夜蛾和棉铃虫抗性，且后代能够稳定遗传。本发明为抗虫植物的研制提供了一种新的抗虫基因，不仅可以使棉花等受体获得多种机制的抗虫性，还利用基因互补的方式加强转基因棉花的抗虫能力，延缓害虫产生抗性，拓宽了棉花抗虫谱，使其获得能够高效杀死主要害虫的广谱抗虫性。

Description

一种抗虫性的苏铁毒蛋白基因、表达载体及应用
技术领域
本发明属于基因工程技术领域，尤其涉及一种抗虫性的苏铁毒蛋白基因、表达载体及应用。
背景技术
小麦、玉米、棉花等作物可持续性发展是保障我国粮食安全的重中之重。其中棉花不仅是世界上最重要的天然纤维作物，也是重要的油料作物和高蛋白粮食作物。
全球每年多达40％的农作物产量因虫害而损失，造成的损失至少为700亿美元。为了抵御害虫侵袭，科学家们在抗虫基因挖掘与抗性机制解析方面取得了一系列重要进展。目前，研究成功的抗虫基因主要有苏云金芽孢杆菌杀虫基因、豇豆胰蛋白酶抑制剂、慈菇蛋白酶抑制剂、淀粉酶抑制剂、营养杀虫蛋白、胆固醇氧化酶、雪花莲凝集素、半夏凝集素、尾穗苋凝集素及蝎毒基因等昆虫神经毒素。在棉花上应用最为成功的是郭三堆等研制的双价转基因抗虫棉新品种，其遗传转化的抗虫基因为Bt杀虫基因GFMCry1A和豇豆胰蛋白酶抑制剂基因Cpti。
虽然现有转抗虫基因棉花能有效控制鳞翅目、鞘翅目、同翅目害虫，但由于这些基因在棉花整个生长期内一直表达，这就有可能使害虫易于对其产生抗性，从而成为转基因棉花发展中的最大威胁。其中，澳大利亚转基因抗虫棉已然对棉铃虫无效。
目前农作物抗病虫育种可用抗性基因资源匮乏，急需挖掘新的抗病虫基因，并利用基因互补的方式加强转基因作物或植物的抗虫能力，同时也应注意延缓害虫产生抗(耐)性。
发明内容
针对现有技术存在的问题，本发明提供了一种抗虫性的苏铁毒蛋白基因、表达载体及应用。
本发明是这样实现的，一种抗虫性的苏铁毒蛋白基因，所述抗虫性的苏铁毒蛋白基因的核苷酸序列为SEQ ID NO.1。
本发明的另一目的在于提供苏铁中所述抗虫性的苏铁毒蛋白基因的同源基因，所述同源基因的核苷酸序列为SEQ ID NO.2，SEQ ID NO.3，SEQ ID NO.4，SEQ ID NO.5。
本发明的另一目的在于提供一种由所述的抗虫性的苏铁毒蛋白基因经密码子优化得到的苏铁毒蛋白优化基因，优化物种为棉花，所述苏铁毒蛋白优化基因的核苷酸序列为SEQ ID NO.6，SEQ ID NO.7，SEQ ID NO.8，SEQ ID NO.9，SEQ ID NO.10。
本发明的另一目的在于提供一种编码所述的抗虫性的苏铁毒蛋白基因的蛋白质，所述蛋白质的氨基酸序列为SEQ ID NO.11，SEQ ID NO.12，SEQ ID NO.13，SEQ ID NO.14，SEQ ID NO.15。
本发明的另一目的在于提供一种编码所述的抗虫性的苏铁毒蛋白基因的杀虫应用或杀虫剂制造，应用范围包括但不限于棉铃虫、小菜蛾、草地贪夜蛾、线虫等植物或作物害虫。
本发明的另一目的在于提供一种应用所述的抗虫性的苏铁毒蛋白基因构建得到的植物或作物表达载体，所述表达载体的构建方法包括：
线性化载体制备：使用限制性内切酶BstBI和MluI双酶切pVM062-3*FLAG载体，使环状载体线性化。
带有同源臂的目标序列扩增：
以正向引物：
5’-gggcggtaccccgggttcgaaATGGTTATGGTAATTGACTATCCAAGTC-3’；
反向引物：5’-cactagttaattaagacgcgtCTATGTCATGCGTAGAATGGCCC-3’
为引物，对苏铁毒蛋白基因进行PCR扩增。
载体酶切产物与PCR产物的检测与回收：琼脂糖凝胶电泳检测目的基因片段与载体酶切产物，使用Quick Gel Extraction Kit回收目的片段。
胶回收产物重组：使用-Basic Seamless Cloning and Assembly Kit进行同源重组，反应体系如下：2×Basic Assembly Mix 5μL；酶切载体XμL；PCR产物YμL；将灭菌水加入至10μL。*10μL反应体系中，载体与各个***片段加入量均在0.01～0.25pmols，载体与各个***片段的最佳摩尔比为1：2；轻轻混合，50℃反应15min，进行转化。测序鉴定：经菌落PCR和质粒PCR筛选阳性克隆，进行测序分析。
进一步，所述扩增体系为：KOD OneTM PCR Master Mix 25μL；上游引物10μM each1.5μL；下游引物10μM each 1.5μL；模板2μL；灭菌水20μL；总体积50μL。
所述PCR的程序为：PCR的程序为98℃5min；98℃30s，50℃30s，68℃3min，30个循环；68℃10min，4℃保存。
进一步，所述转化方法包括：
(1)在冰上融化Trans1-T1感受态细胞，静置2-5分钟，观察细胞是否已解冻，用手指轻弹管底1-2次；在50μL细胞中加入2μL的重组产物，轻轻弹动离心管壁混匀后，在冰上放置30min；
(2)42℃水浴中热激30s后马上转至冰上冷却2min；加入450μL常温无抗LB液体培养基后，于37℃摇床中250rpm培养1h；
(3)5000rpm离心2min，收集菌体，留100μL液体重悬，均匀涂布于含有卡那霉素抗性的LB固体平板上，37℃培养箱过夜培养。
本发明的另一目的在于提供一种应用所述的抗虫性的苏铁毒蛋白基因的植物或作物的抗虫方法，所述植物或作物的抗虫方法是将含有目标序列的植物表达载体导入到植物或作物细胞内，并由此获得对害虫有抗性或杀伤能力的转基因植物或作物及其后代，包括种子和任何部分的植物或作物组织；或者通过同源或异源表达的方法获得该蛋白施用于植物或作物，并由此植物或作物获得虫害防御能力。
其中，所述植物或作物包括但不限于棉花、玉米、小麦和水稻等。
本发明的另一目的在于提供一种转基因棉花植株的苏铁毒蛋白基因表达检测方法，所述转基因棉花植株的苏铁毒蛋白基因表达检测方法包括：取转基因棉花纯合株系叶片，提取叶片总蛋白，用于Western Blot检测；具体包括：
(1)取新鲜样品1～2g，将样品放在研钵中用液氮研磨；研磨后置于10mL试管中，并加入蛋白提取液4℃摇匀30min；
(2)于12000rpm 4℃离心20min；提取上清液，样品制备完成；
(3)取上清加入Loading Buffer，98℃煮5～10min后用SDS-聚丙烯酰胺凝胶电泳法测定蛋白质表达情况。
进一步，所述蛋白提取液包括0.01M PBS pH＝8，1mM PMSF，2mM DTT。
结合上述的技术方案和解决的技术问题，本发明所要保护的技术方案所具备的优点及积极效果为：
针对农作物抗病虫育种可用抗性基因资源匮乏，本发明提供了一种新的能够使植物或作物(包括但不限于棉花、玉米、小麦和水稻等)产生抗虫性的苏铁毒蛋白基因，本发明提供了一个明确的转基因棉花株系，其具有草地贪夜蛾和棉铃虫抗性。
本发明提供了一种新的抗虫性的苏铁毒蛋白基因，构建相关植物表达载体，利用农杆菌介导法转化棉花，获得转基因棉花株系，具体步骤包括对抗虫性的苏铁毒蛋白基因进行克隆，测序分析；构建所述基因的超表达载体；采用农杆菌介导法进行棉花遗传转化；其中，所述超表达载体中抗虫性的苏铁毒蛋白基因是由35s启动子驱动。本发明提供的毒蛋白基因来自苏铁，将其导入到棉花中，使棉花获得草地贪夜蛾和棉铃虫抗性，且后代能够稳定遗传。
本发明能够进一步提供或利用上述抗虫基因获得其它转基因作物和相应的种子，也可以利用有性杂交的方式将本发明的抗虫基因转入相同背景的其它品种或相近物种，以达到加快各物种抗虫种质创新的效果。
本发明描述了小菜蛾、草地贪夜蛾、棉铃虫等农业主要害虫注射苏铁毒蛋白及转基因植株喂养存活情况，表征如下：
(1)取龄期一致的三龄第二天的小菜蛾(平均体重1.33mg/头)。每头小菜蛾注射2.03μg苏铁毒蛋白，设置对照组，注射等体积(207nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果表明注射苏铁毒蛋白后，小菜蛾平均死亡率为89.30％，显著高于对照组的死亡率7.78％。
(2)取龄期一致的二龄第二天的棉铃虫(平均体重是2.06mg/头)。每头棉铃虫注射3.38μg苏铁毒蛋白，设置对照组，注射等体积(345nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果表明注射苏铁毒蛋白后，棉铃虫平均死亡率为37.78％，显著高于对照组的死亡率5.56％。
(3)取龄期一致的二龄草地贪夜蛾(平均体重1.01mg/头)，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫，25℃培养4天统计死亡率。结果表明喂食转基因棉花4天后，草地贪夜蛾的平均死亡率为61.11％，远高于喂食“百棉1号”的平均死亡率24.07％和喂食TM-1的平均死亡率12.96％。
(4)取龄期一致的二龄棉铃虫(平均体重3.9mg/头)用于棉铃虫的取食实验。实验前，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫，25℃培养4天统计死亡率。结果表明喂食转基因棉花4天后，棉铃虫的平均死亡率为59.26％，远高于喂食“百棉1号”的平均死亡率29.63％和喂食TM-1的平均死亡率14.81％。
本发明组装了苏铁高质量基因组，通过基因组分析发现了水平基因转移现象，即苏铁基因组中发现了来自于真菌的基因。通过分析发现该基因编码蛋白含有细胞毒素结构域，具有杀虫效果。而且水平基因转移是一个自然的转基因过程，这些转移的基因通常对植物无害，因此本发明选择该基因作为抗虫候选基因。
本发明苏铁毒蛋白基因来源于发明人组装的苏铁基因组，通过基因组分析并提取出该基因序列，设计引物并进行PCR克隆得到。
本发明所提供的苏铁毒蛋白基因基因可显著提高植物(包括但不限于棉花、玉米、小麦和水稻等)抗虫性的一个基因，由于其来源于植物自身，转化到其他植物后一般不存在毒害效应。该基因转化棉花后，不仅可以使棉花同时获得草地贪夜蛾和棉铃虫抗性，还可以利用基因互补的方式加强转基因棉花的抗虫能力，拓宽了棉花抗虫谱，使其获得能够高效杀死主要害虫的广谱抗虫性。本发明成功克隆了苏铁毒蛋白基因，提供了该基因的核苷酸序列和编码蛋白质氨基酸序列，为植物抗虫种质的创制及新品种的选育提供理论基础和应用参考。
本发明的技术方案转化后的预期收益和商业价值为：本发明的技术方案转化后的预期收益与后续转基因棉花品种的审定和推广面积有关，我国抗虫棉面积自2006年以来，稳定在350万公顷左右，本发明将通过传统遗传实验，培育不同背景的抗虫棉，并积极推进审定棉花抗虫新品种，商业价值十分可观。
附图说明
为了更清楚地说明本发明实施例的技术方案，下面将对本发明实施例中所需要使用的附图做简单的介绍，显而易见地，下面所描述的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下还可以根据这些附图获得其他的附图。
图1是本发明实施例提供的苏铁毒蛋白基因的PCR扩增结果示意图。
图2是本发明实施例提供的构建的植物表达载体示意图，利用35S启动子驱动苏铁毒蛋白基因，该植物表达载体带有壮观霉素抗性基因。
图3是本发明实施例提供的转基因棉花植株示意图。
图4是本发明实施例提供的对棉花遗传转化植株叶片中苏铁毒蛋白基因的表达进行PCR检测的示意图，图中可以清晰看到苏铁毒蛋白基因在棉花叶片中表达。
图5是本发明实施例提供的对棉花遗传转化植株叶片中苏铁毒蛋白基因的表达进行Western Blot检测的示意图，图中可以清晰看到苏铁毒蛋白基因在棉花叶片中表达。
图6是本发明实施例提供的对棉花遗传转化植株叶片进行抗虫检测的示意图，图中可以清晰看到苏铁毒蛋白基因显著抑制草地贪夜蛾和棉铃虫的生长发育。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
针对现有技术存在的问题，本发明提供了一种抗虫性的苏铁毒蛋白基因、表达载体及应用，下面结合附图对本发明作详细的描述。
一、解释说明实施例。为了使本领域技术人员充分了解本发明如何具体实现，该部分是对权利要求技术方案进行展开说明的解释说明实施例。
本发明所涉及的KOD OneTM PCR Master Mix购于东洋纺(上海)生物科技有限公司；Quick Gel Extraction Kit、/>-T1 Cloning Kit、/>-Basic Seamless Cloning and Assembly Kit购于北京全式金生物技术有限公司；质粒小提试剂盒购于OMEGA有限公司；植物RNA提取试剂盒购于天根生化科技(北京)有限公司；RNA反转录试剂盒购于诺唯赞生物科技有限公司；引物合成、基因测序由上海生工生物工程有限公司完成；基因优化和合成由河南尚亚生物技术有限公司完成。
实施例1：克隆苏铁毒蛋白基因
1.苏铁RNA模板的获得
取苏铁嫩叶，使用植物RNA提取试剂盒提取RNA。取苏铁嫩叶约1g，液氮速冻，放入研钵中研磨至细粉状，之后按照植物RNA提取试剂盒说明书进行RNA提取。RNA浓度用NanoDrop可见光分光光度计(Thermo Fisher，美国)测定。
取刚提取的苏铁RNA，按照反转录试剂盒说明书将RNA反转录为cDNA。
利用反转录得到的cDNA为模板，SEQ ID NO.16：以正向引物：5’-ATGGTTATGGTAATTGACTATCCAAGTC-3’；反向引物：5’-CTATGTCATGCGTAGAATGGCCC-3’为引物对苏铁毒蛋白基因进行PCR扩增，扩增体系为：
KOD OneTM PCR Master Mix 25μL
上游引物(10μM each)1.5μL
下游引物(10μM each)1.5μL
模板2μL
灭菌水20μL
总体积50μL
PCR的程序为98℃5min；98℃30s，50℃30s，68℃3min，30个循环；然后68℃10min，4℃保存。PCR产物用琼脂糖凝胶电泳检测(见图1)，之后用Quick GelExtraction Kit纯化回收PCR产物，然后用/>-T1 Cloning Kit将PCR产物连入T载体，转化大肠杆菌DH5α，利用KOD OneTM PCR Master Mix做PCR检测，挑选阳性克隆送至上海生工生物有限公司测序。经过序列比对，测序正确的克隆利用质粒小提试剂盒提取质粒，并将其被命名为p-T_Suit，序列信息见SEQ ID NO.1：
atggttatggtaattgactatccaagtctcagagcttcgttatatgccactaagagtgcggtcccatgtcgcccgcttccgggcaatccagagcgggtgtcgcgccgggtatcacgcagaggcgcgggagagatcgggtcccatgcagatttctggatctcccgcgccaaggcatccgccatccggattttcccgcgtcaagagggtccgctccgtgaatcccggagaagattctatcttctccgcgaagcggtgttcaccggccgcaggaccgcctgggccgctaatccagcggcccaacgggcagcatgggaccgcaagagatcccctttcttcggaaggtcttccgatgatattcattcattcagcaagaatccagaagaattcttgactcaaaacactctcagctgcagtttattaactcgcaccgctcagaccgtcacaaaaagcccagtattgcccgcccaaggttgggtcaaagtgggtcgtgtgccaggaagaggagacgctctctttgttgagctggttgccgagaaatcagatgagacagtgaaggcgtacttacttggctacaatggtaagaacgactcccccgagccggcatacgttgatattccaaagaggggcgccgagaacggaacatttttgtttaccgtggatctctcgggctgctccgttgttgtgacacagttggacgagcagacataccgtgttttccacgacaaacgggtgaacagctccattctgtacgacaacgtcgtaatgtatgtggatttcaatcactaccgctatcccagagcccacggacacgggagagatacgggacaagcggtcgtctgtatgcaattcgccgacggagtgtggaaattgtttctccagcggcacgagacggcgaattctctcccaaagatatggtcccgtggcgatggcaaaccggagattttcagcgacgccgaaccccgtccacatatggaagatggcataaaggaatttctcaatcggcgtcgagctttgcaagacgaattgaagcacttcgccaacacgcttgaagtggacccggacatagtgtccaacgctgtcgacggcgaatacgaaggcggccagttttccaggaacgacgatgcaattcgtggatggaacgaattacgtgcaagcatgagacagaaaatggctgaaaggaagaaactgttgcaaacaaagaaaggtatgctagactcattcaacaacccggatcaccccctctataaatttcgcaaacaagtgctcgacgagctcaaggaaatgagtgcatacgacaacaccttggaggcatccgagattgcggaccgtacttggctgtggttggagatcaaaaaagccaaaggtacgaatgcagtggtaaacagtggagcagccccgaagcagccgagtcttaccgttggtgaacgctacgcagagatggaggcggccctttggatgcgtagcgacacagcggcgtttgagcaagggtatgaatcttatcaaaccgttcgcgtgccgggttacaatgaagatatgacatccattgcaatgaagaagttgttcgttgagagcagtctgactgacgagcagctgggcgccttgtgcaagcgtatatccattgctagcgatcaagagaaccatcttctgacacagaagacgcaggagattgagagcactttcaaacaattttcagatggcagctttttggagtcggcgcctcaggatttcttcctgagacgagtcggtgataaatctggaggacgctgctatcctcttgtaagggtaatggccgtcgcactgtctgaaggagaagacgttgcccagaattttatggagaacctgaggacattgtctaggaatccagagtattcaggcgaatcggcaagttcacaagccacaaatgcagggttgttcagagatgccctggccggcctgcacgcgaatgtggaagccattgccgcatctgagaaactacctgctgtgtttggactgcccgatgttgctacaatgttgagccaagcgagcgaaagcaaggtttacgctttgaataccgaaacacacagcatgctggttgcggtgattgtagacaaacagagtctggcgcgcaagtattacttctatgatcccaactttctcatggccggtttctccgacataactcgtttctcggaagccctcaagtatcactttgtgaatctccagttcgccggaaactacaaagcgtttggcacagcggaggctccccagtttaacctggtccttattgatacgaccgccatggaggatgcgcatgttgacggagggatcaccgtgaaacaattgagcgagggcgttgatcttactaatgtctttgacagcaccaaaaaggctcgtgtggtaactgaggcagcagctcgcttggctgaagaccaacaactgcgcactggcctcaccataatagaggctgaccagtgggcaaacaggtggcaagatgccgtcatacgattgaaactcaaggaaggattaagtggagagtggatgccattgctggagaaaatgcaagaaaacgcagatggttcttatcagattgatttcatcaacaccaagaatactggggaaatccgtggggtttccactagagatgcgacgttcaaagaattcaaatcctacctggataaaaatttagacgtattgcataaggatttcatctttcaggatggagttttcaaaccactggagggagagcccattgacgggttgaatgcaatgttcgcggtccaaatgattatagattggttcaaccgggggactaatgctgaggaagatgcggcctcaggatccaccaatgcccaactttccctagcgattaaagtgcacagctacattaacatggcccaagtagcgcatactgcagttacagatgttgccaaggtatacaaccttgtaaagtcagcactgtatggcgagcaggtagctggggagacatcgctgttcacatttgggaaggcgttgaaatttgcagcaacagaagggttgggagtcttactaggggcgtcctttgtgatcttggatgcctaccagctgtccctcgtggaaaacgatgtgcagaaggccattatcggcacacaactagcctttgattccgcaagctttctagcgggtattggtgcaattgttgctggagcatcgggcgtgggcatgattttgggcggagctggcgtggtattgggaggcctgggagttggctttggagccttagctcaggcattcggggaaatcacagcagacgcccaactggtgggcaagtacttcgcactcttggacgaggcataccgcggtggcggatacagatacgaatctaaagacaaggcattgatgcctcttgcaggagcagtcatcgacaaaatacgactggccgacaaacatctccaagttcatttcgacagccaattcatatatcgaacccatcatggcagcacaggatccggcaaaattaattacttcttctgggctggcgacatgccgcagatggtccacgatcgctcccaagctatcaatgttcgagagcaaattgggtataagaacgataatgctactttggagctaccggaggatgcctctgatctgatcctgcctgccacgcccaagtcatttatcacttaccgatacgagattctaccattcgctacttcccgccacgatacgggtttcgatgtcattagacgcctggaagaagactacagattcgattacgatttctacatcttcccatctgaatacacaatcagaaccatcaaacacgagtacgtagaaacaaaggtggcagtagtcctggataggcgctctttgcgactgggagcacctgagcttctcaaggagcaacatggctatttgatctatgagatcgaggggaccggaggacaatacgttatatgtcttaaccagggatccactgtcacactgaagaccaccggatcagaggcaaataaaacattatggatacttgatgcccgacaactcattgcaggcaacgattcaatcacggtctctcaagatagcatcaccatcggcagcacaacagtccatctccgtaaccaagatttcgattgcctacagatcgtcatgagtcgggaggagcttctccaagtggacttggcgtctggagaagcccgcgtgttttcagaagatgccagtaaatggctcgacaccaaagaaacaatccatgaacatctccataagttggctgaagaacacaggttgcatggccagtttgtagtggtgaacaattattatcgtggagtgggccgtgcctactatgatgctgctcacgatcggatgttgtatacagatgtggaagatgaaactctgactcgggatgctgttcttgccggcgttacaggcgaagatgcctacttctacaatgtaaagcagaatgccctgtggcgagtgaatgcaggcacacaccaagtaataaccaaatactatccgacgttactggaggaccctgacagcaacatggcaagagttgagcagcatggcaacatcgtcgtgatggccatgagcaggctcaaccacccggacaagggagtaaatggaatcatctatctcgttcatcaagaaagcatcgtgcttactgctgtgatcggcaattcagagatgctcaaacgtcttcgccaaacgagtacaattagcttaacagaattgcgaggtgtcaatgacatggctctggtttccacgatggacagattacccggccaaaaggttgtcgctaccaacgctgagtttgtgacacttctggctaaggatgagcaaggagccaatatcaaggagcagacggtctattgccaacaaggtacgggccagaccaacgatacatctattgaaagcatcccgattccagggctctctaatcttttaaccgtgtacggtgaagtattcgctacaactaatgatggtcgcatccttcgcctgcttggggacggctcaacatgtctcgagggcatcaatgagcattggctcatcaagcatccaaaatggtggcaggacttggctaccttggaggcagagaaagccacgacagttgcagtattcggtgttagagacctacaaaatgcagtggtgccaatttggtaccacaacggaaagatcgcggtggcctcatcaaaactgtatggcaaacaactgcaatttttggggtttaacagcaacggtgaagcttggttgttcgacttggacgacggcattctgtatagccagccgcttgtggatagctttctgtatgcaatctttagtttggacgggaagcttattgcaccaatctttattccacaagtggagcaaatgctcagcggccagcatctcagaaaccctgtcatcgtggacggccgtttgcatgtgactaccatagacggtgttgttgttggatttgacaagaagtcgccaactctggttgcagtgaacaaggcctggcagatatctcgtcagaaccgattagaagaggccctcggaaacctgctgtccaattggtctgatcacaatgatgcgattgtgttgcagggtgatccagaatcagcgccggcatggtattatgttccatcaagagaaatagtgaccgccaatggtctaacatgggcggatcatcctctatggctaggaatggctgtcggtgctgcaacgccatacatctacagcacatcccgacgccgtgtgtatgttgtggagagcaaaaacatggccaaagacaaaggagaattcgcagcagcacattgctttgcaaaatcacttgtgctacagagcatcgttcccgaggaaataacgattccgacgctggctggcatcgatttcgcactcgttgcagcgacaaatgcaaccattgtaatagaccgagacaattggaagcattacaaggccattgtgctcgacaacaaggtggatcaggacggtggcgctaaggtggtagtcggcagtgagattcccatgaaagttctggtgcgcaaatttcagggaaatctggagttcttcgatccaaacaatggtaatgttgttatactccagaatgtgttgaagggagacggagcttgttgccagatagagatcaaagtaggcgatgctgagactatgcttgtatccgatttctcggagatattctcaaagacgcatcctctggtggcaagccaagtatttgagcttggggatgtgattgcagtcacaaaacacaaatcggactatgcggatctgtgggccattctacgcatgacatag
本发明提供由所述的抗虫性的苏铁毒蛋白基因在苏铁中的同源基因，所述同源基因的核苷酸序列如下：
SEQ ID NO.2：
ATGGAGGAGGCTGACATGGAGAACCGTGGATACGAAGAACGGCACTCACAGTCACAGTCTGCTACACCGCTCCGCCGAAGGAAGAGATCCCCTTTCTTCGGAAGGTCTTCCGATGATATTCATTCATTCAGCAAGAATCCAGAAGAATTCTTGACTAAAAACACTCTCAGCTGCAGTTTATTAACTCGCACCGCTCAGACCGTCACAAAAAGCCCAGTATTGCCCGCCCAAGGTTGGGTCAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGCTCTCTCGGGCTGCTCCGTTGTTGTGACACAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCACGGACACGGGAGAGATACGGGACAAGCGGTCGTCTGTATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGAACCCCGTCCACATATGGAAGACGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAATTGAAGCACTTCGCCAACACGCTTGAAGTGGACCCGGAGATAGTGTCCAACGCTGTCGACGGCGAATACGAAGGCGGCCAGTTTTCCAGGAACGATGATGCAATTCGTGGATGGAACGAATTACGTGCAAGCATGAGACAGAAAATGGCTGAAAGGAAGAAACGGTTGGAAAGAAACAAAGGTATGCTAGACTCATTCAACAACCCGGATCACCCCCTCTATAAATTTCGCAAACAAGTGCTCGACGAGCTCAAGGAAATGAGTGCATACGACAACACCTTGGAGGCATCCGAGATTGCGGACCGTACTTGGCTGTGGTTGGAGATCAAAAAAGCCAAAGGTACGAATGCGGTGGTAAACAGTGGAGCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGGTATGAATCTTATCAAACCCTTCGCGTGCCGGGTTACAATGAAGATATGACATCCATTGCAATGAAGAAGTTGTTCGTTGAGAGCAGTCTGACTGACGAGCAGCTGGGCGCCTTGTGCAAGCGTATATCCATCGCTAGCGATCAAGAGAACCATCTTCTGACACAGAAGACGCAGGAGATTGAGAGCACTTTCAAACAATTTTCAGATGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTGCACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGTTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATTGCCGGTTTCTCCGACATAACTCGTTTCTCGGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGCGGATGCGCATGTTGACGGAGGGATCACGGTGAAACAATTAAGCGAGGGCGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGACAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACAGGTGGCAAGATGCTGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGTTGGAGAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGACTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTAGACGTATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCTGTCCAAATGATTATAGATTGGTTCAACCGGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAATCTTGTAAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGAGGCATCGCTGTTAACATTTGGGAAGGCGTTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATCGGCACACAACTAGCCTTTGATTCCGCAAGCTTTCTAGCGGGTATTGGTGCAATTGTTGCTGGAGCATCGGGCGTGGGCATGATTTTGGGCGGAGCTGGCGTGGTATTGGGAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGGAAATCACAGCAGATGCCCAACTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACCGCGGTGGCGGATACAGATACGAATCTAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACATCTCCAAGTTCATTTCGATAGCCAATTCATATATCGGACCCATCATGGCCTCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGCTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATATTCGAGAGCAAATTGGATACAAGAACGATCATGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCAATACCAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATCAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATTAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTATATGTCTTAACCAGGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCAAATAAAACATTATGGATACTTGATGCCCGGCAACTCATTGCAGGCAACGATTCAATCACGGTCTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTACAGATCGTCATGAGTCGGGAGGAGCTTCTCCAAGTGGACTTGGCGTCTGGAGAAGCCCGCGTGTTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACACAGGTTGCATGGCCAGTTTGTAGTGGTGAACAATTATTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCACGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGTTACTGGAGGACCCTGACAGCAAAATGGCAAGAGTTGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACCCCGACAAGGGAGTAAATGGAATCATCTATCTCGTTCATCAAGAAAGCATGGTGCTTACTGCTGTCATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGTACGATTAGCCTAACAGAATTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAAATTACCCGGCCAGAAGGTTGTCGCCACCAATGCTGAGTTTGTTACTATTCTGGCTAAGGATGAGCAAGGGAGCCAAGTATGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAACCTTGACTGTTTCATCCCACAAAGTGGCTGGACAGGATGTATTCTACTTTTACAGTATCAAGGAGCAGACGGTCTATTGCCAACAAGGGACGGGCCAGACCAACGATACATCTATTGAAAGCATCCCGATTCCAGGGCTCTCTAATCTTTTAACCGTGTACGGTGAAGTATTCGCTACAACTGATGATGGTCGCATCCTTCGCCTGCTTGGGGATGGCTCAACATATCTTGACGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAGTTGCAGTATTCGGTGTTAGAGACCTACAAAATGCAGTGGTGCCAATTTGGTACCACAATGGAAAGATCGCGGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTAACAGCAACGGTGAAGCTTGGTTGTTCGACTTGGACGACGGCATTCTGTATAGCCAGCCGCTTGTGGATAGCTTTCTGTATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAATCTTTATTCCACAAGTGGAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGTTTGCATGTGACTACCATAGACGGTGTTGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAGGCCTGGCAGATATCTCGTCAAAACCGATTAGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCATCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTTGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAGCACATCCCGACGCCGTGTGTATGCTGTGGAGAGCAAACACATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGAGGAAATAACGATTCCAACGCTGGCAGGCATCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATACACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGGGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCGGAATGTGTTGAAGGGAGACGGAGCTTGTTCCCAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTTGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAG
SEQ ID NO.3：
ATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTACACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGCTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCAGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGCGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGGGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACCGGTGGCAAGATGCCGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAAAGAATGCAAGAAAACGGACATGGTTCATATCAGATTGAATTCCTCAACACCAAGAATACTGGGGAAACCCGTTGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTTGACGCATTGCGTAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGATGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCGGTCCAAATGATTATAGATTGGTTCAACCCGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAATCTTGTAAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGAGGCATCGCTGTTAACATTTGGGAAGGCGTTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATCGGCACACAACTAGCCTTTGATTCCGCAAGCTTTCTAGCGGGTATTGGTGCAATTGTTGCTGGAGCATCGGGCGTGGGCATGATTTTGGGCGGAGCTGGCGTAATATTGGGAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGCAAATCACAGCAGATGCCCAACTGGTGGGCGAGTACTTCGCACTCTTGGACGAAGCATACAGCGGTGGCGGATACAGATACGAATCCAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACGTCTCCAAGTTCATTTCGACAGTCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAACTACTTCTTCTGGGTTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATAGGGTATAAGAACGATAGTGCTACTTTGGAGCTACCGGAGGATGCCTCCGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATCAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTATATGTCTTAACCACGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCGAATAAAACATTATGGATACTTGATGCCCGGCAACTCATTGCAGGCAACGATTCAATCACGGTCTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTGCAGATCGTCATGAGTCGGGACGAGCTTCTCCAAGTGGACTTGGCGTCTCGAGAAGTCCGCGTGTTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGGAGAACACAGGTTGCATGGCCAGTTTGTAGTGGTGAACAATTATTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCACGATCGGATGTTGTATACAGATGTGGAAGATGAGACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAATCAGAATGCCCTGTGGCGAGTGAATGCAGACACACACCAAGTAATAACCAAATACTATCCGACGCTCCTGGAGGACCCTGACAGTAAAATGGCAAGAGTCGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACCCCGACAAGGGAGTAAATGGAATCATCTATCTCGTTCACCAAGAAAGTATGGTGCTCACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGCACGATTAGCTTAACAGAATTGCGAGGCGTCAATGACATGGCCCTGGTTTCCACGATGGACAGATTACCCGGCCAAAAGGTTGTCGCCACCAACGCTGAGTTTGTGACACTTCTGGCTAAGGATGAGCAAGGGAGCCAAGTCTGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAACCTTGACTGTTTCATCCCACAAAGTGGCTGGACAGGATGTATTCTACTTTTACAGTATCAAGGAGCAGACGGTCTATTGCCAACAAGGTACGGGCCAGACCAACGATACATCTATTGAAAGCATCCCGATTCCAGGGCTCTCTAATCTTTTAACCGTGTACGGTGAAGTATTCGCTACAACTAATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATATCTTGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAGTTGCAGTATTCGGTGTTAGAGACCCACAAAATGCAGTGGTGCCAATTTGGTACCACAATGGAAAGATCGCAGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTAACAGCAACGGTGAAGCTTGGTTGTTCGACTTGGACGACGGCATTCTGTATAGCCAGCCGCTTGTGGATAGCCAAGCTGTGGATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAAACCTTATTCCACAAGTGGAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGCTTGCATGTGACTACCATAGACGGTGTTGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAAGCCTGGCAGATATCTCGTCAGAACCGATTAGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCACCGGCATGGTATTATGTTCCATCAAGGGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCTATACATCTACAGCACATCTCGACGCCGTGTGTATGCTGTGGAGAGCAAAAAGATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGAGGAAATAACGATTCCAACGCTGGCTGGCACCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATAGACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTCCAAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTTGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAG
SEQ ID NO.4：
ATGGAGGAAGTTGACATGCAAAACCGTGGATACGAAGAATGGCACTCACAGTCACAGTCTGCTACAACGCTCCGCCGAAGGAAGAGATCCCCTGTCTTCGGAAGGTCTTCCGATGACATTCATTCGTTCAGCAAGAATCCAGAAGAATTCTTGACTCAAAACACTCTAAGCTGCAGTTTATTAACTCGCACCGCTGAGACCGTCACAAAAAGCCCAGTATTGCCCGAACGAGGTTGGGTTAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGCTCTCTTTGTTCAAGTGGTTGCCGAGCAATCATATGACACCGTGAAAGCGTACTTACTTGGCTACAATGGTGAGAACGACTCCCCCAACCCGGCATATGTTGATATTCCAAAGAGGGCCGCCGAGAACGGAACATTTTTGTTTACCGTTGATCTCTCGGGCTGCTCCGTTGTTGTGACGCAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCATGGACACGGGAGAGATACCGGACAAGCGGTCGTCTGTATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGAACCCCGTCAACATATGGAAGACGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAGTTGAAGAAATTCGCCGACATGTTTGGAGTGGACCCGGAGATAGTGTCCAACGCTGTCGACGGCAAATACAAGGGTGGCCAGTTTTCCAGGAACGATGATGCAATTCGTGGATGGAACGAATTACGTGCAAGCATGAGACAGAAAATGGCCGAAAGGAACAAAGTGTTGCTAACAATGAAACTGGAGCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGCTGGGCGCCTTGTGCAAGCGTATATCCATCGCGATCAAGAGAACCATCTTCTGACACAGAAGACGCAGGAGATTGAGAGCACTTTCAAACAATTTTCAGATGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAAGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTGCACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGCTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCAGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAACCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGTCATGGCGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGGGTTGATCTTACTAATGTCTTCGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACCGGTGGCAAGATGCCGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAAAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGACTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGGGACGTTCAAAGAATTAAAATCCTACCTGGATAAAAATTTAGACGCATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCAGTCCAAATGATTATAGATTGGTTCAATCGGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAGCCTTGTGAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGGGGCATCGCTGTTAACATTTGGGAAGGCGCTGAAACTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGCCTGTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATTGGCACACAACTAGACTTTGATTCCGCAAGCTTTTTAGTGAGTATCGGGGCAGCAGTTGCTGGAGTATCGGGCGTGGGCATCGTTTTGAGCGGAGCTGGCGTGTTATTGGCAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGGAAATCACAGCAGACGCCCAGCTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACCGCGGTGGCGGATACAGATACGAATCTAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACATCTCCAAGTTCATTTCGACAGCCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGTTGGCGACATGCCGAAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATTGGGTATAAGAACGATAGTGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACCACAGATTCGATTACGTTTTCTACATCTTCCCATCTGAATACACAATTAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCCGGATAGGCGCCCTTTGCGACTGCGAGCACCCGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATTGAGGGGACCGGAGGAGAATACGTTATATGTCTGAACAAGGGATCCACTGTCACACTGTTCACCACCGGGTCAGAGGCAAATAAAACATCATGGATACTCGATGCCCGGCAAATCATTGCAGGCAACGATCCAATCATAGTCTCTCAAGATAGCATCACCATGGGCAGCACAACCGTCCATCTCCGTAACCATGATTTCGATTGCCTACGGATCGTCACGAGTCGGGAGGAGCTTCTCCAAGTGGACTCGGCGTCTCGACAAACCCGCGTGCTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACATAGGTTGCTTGGCCAGTTTGTAGTGGTGAACAATTATTCCGTGCAAGAACAAAGTGACATCGGCACGCTTTATCGTGGAGTGGGCCGTGCTCATGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAGGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGGTACTGGAGGACCCTGACAGCAAAATGGCAAGAGTTGAGCAGCGTGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACTCCGACAAGGGAGTAAATGGAATCATCTATCTCGTTCATCAAGAAAGCATGGTGCTTACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAGCGAGTACGATTAGCCTAACAGAACTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAAATTACCCGGCCAGAAGGTTGTCGCCACCAATGCTGAGTTTGTCACTATTCTGGCTAAGGATGAGCAAGGGAGCCAAGTCTGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAACCTTGACTGTTTCATCCCACAAGGTGGCTGGACAGGATGTATTCTACTTTTACAGTATCAAGGAGCAGAGGGTCTATCGCCAACAAGGTACGGGCCAGACCAACGATACATCTGTTGGAAGCATCCCGATTCCAGGACTCTCTAATCTTTTCACCGTGTACGGTGAGGTATTCGCTACAACTAATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATATCTTGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAATTGCAGTATTCGGTGTTAGAGACCCACAAAATGCAGTGGTGCCAATTTGCAACGGTGAAGCGTGGTTGTTCGACTTGGACGGCGGCATTCTGTATAGCCAGCCGCTCGTGGATAGCCAAGCTGTGGATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAAACCTTATTCCACAAGTACAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTCGACGGCCGTTTGCATGTGACTACCATAGACGGTGTCGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGGGAACAAGGCCTGGCAGATATCTCGTCAGAACCGATTGGAAGAGGCCCTCAGAAATCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCATCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAGCACATCTCGATGCCGTGTGTATGCTGTGGAGAGCAAAAACATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGCGGAAATAACGATTCCAATGCTGGCCGGCATCGATTTCGCACTCGTTGCAGCGACAAAAGCAACCATTGTAATAGACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCCAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTCCCAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTCGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCGAGCCAAGTATTTGAACTTGGGGATGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAGCCTATATATCCCACGCATATCTTCACCGATGGCCTATATATGTCATTTCAACGTTGATAGCGTAG
SEQ ID NO.5：
ATGGAGGAGGCTGACATGGAGAACCGTGGAAACGAAGAACGGCACTCACAGTCACAGTCTGCTACACCGCTCCGCCGAAGGAAGAGATCCCCTGTCTTCGGAAGGTCTTCCGATGATATTCATTCATTCAGCAAGAATCCAGAAGAATTCTTGACTAGAAACACTCTCAGCTGCAGTTTATTAACTCGCACCGCTGAGGCCGTCACAAAAAGCCCAGCATTGCCCGCCCAAGGTTGGGTCAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGTTCTCTTTGTTCAGCTGGTTGCCGAGAAATCAGATGAGACAGTGAAGGCGTACTTACTTGGCTACAATGGTGAGAACGACTTCCCCAAGCCGGCATACGTTGATATTCCAAAGAGGGGCGCCAAGAACGGAACATTTTTGTTTACCGTTGATCTCTCGGGCTGCTCCGTTGTTGTGACACAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCACGGACACGGGAGAGATACGGGACAAGCGGTCGTCTGCATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGATCCCCGTCCACATATGGAAGACGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAGTTGAAGAAATTCGCCGACATGTTTGGAGTGGACCCGGAGATAGTGTCCAATGCTGTCGACGGCAAATACGAGGGCGGCCAGTTTTCCAGGAACGATGATGCAATTCGTGGATGGAACGAATTACGTGCAAGAATGAGACAGAAAATGGCTGAAAGGAAGAAACTGTTGGAAACAAAGAAAGGTATGCTAGACTCATTCAACAACCCGGATCACCCCCTCTATGAATTTCGCAAACAAGTGCTCGACGAGCTCAAGGAAATGAGTGCATACGACAACACCTTGGAGGCATCCGAGATTGCGGACCGTACTTGGCTGTGGTTGGAGATCAAAAAAGCCAGAGGTACGAATGCGGTGGTAAACAGTGGATCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGGTATGAATCTTATCAAACCGTTCGCGTGCCGGGTTACAATGAAGATATGACATCCATTGCAATGAAGAAGTTGTTCGTTGAGAGCAGTCTGACTGACGAGCAGCTGGGCGCCTTGTGCAAGCGTATATCCATCGCTAGCGATCAAGAGAACCATCTTCTGAGACAGAAGACGCAGGAGATTGAGAACACTTTCAAACAATTCTCAGACGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTCAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTTGCCGGCCTGCACGCGAATGTGGAAGCCATCGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGTTGCGGTGATTGCAGACGAACAGAGTCTGGCGCCCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCAGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCTGGAAATTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGCGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGGGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACCGGTGGCAAGATGCCGTCATACGATTGAGACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAAAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGACTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTAGACGCATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTAGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCAGTCCAAATGATTATAGATTGGTTCAATCGGGGGACTAATGCCGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCACTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAGCCTTGTGAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGGGGCATCGCTGTTAACATTTGGGAAGGCGCTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATTGGCACACAACTAGCCTTTGATTCCGCAAGCTTTTTAGTGAGTATCGGGGCAGCAGTTGCTGGAGTATCGGGCGTGGGCATCGTTTTGAGCGGAGCTGGCGTGTTATTGGCAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGCAAATCACAGCAGATGCCCAACTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACAGCGGTGGCGGATACAGATACGAATCCAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTAGCCGACAAACATCTCCAAGTTCATTTCGACAGCCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGCTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATCGGGTATAAGAACGATAGTGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTTGCTACTTCCCGCCACGATAGGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATCAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTGTATGTCTTAACCACGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCAAATAAAACATTATGGATACTTGATGCCCGGCAACTCATTGCAGGCAACGATTCAATCACGGTTTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTACAGATCGTCATGAGTCGGGAGGAGCTTCTCCAAGTGGACTTGGCGTCTCGACAAGCCCGCGTGCTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACATAGGTTGCTTGGCCAGTTTGTAGTGGTGAACAATTATTCCGTGCAAGAGCAAAGTGACATCGGCACGCTTTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCATGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGGTACTGGAGGACCCTGACAGCAAAATGGCAAGAGTTGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCAGCCCGACAAGGGAGTAGATGGAATCATCTATCTCGTTCATCAAGAAAGCATGGTGCTTACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGTACGATTAGCTTAACAGAATTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAAATTACCCGGCCAGAAGGTTGTCGCCACCAATGCTGAGTTTGTGACTATTCTGGCTAAGGATGAGCAAGGGAGCCAAGTCTGTTTTTGGCTGCGCACCGTGGATGGAGTTCTAATCAAGCCGAGCATCCAGCCACCCATACCAACGGACCTAGCCTTGACTGTTTCATCCCACAAGGTGGCTGGACAGGATGTATTCTACTTTTGCAGTATCAAGGAGCAGACGGTCTATCGCCAACAAGGTACGGGCCAGACCAACGATACATCTGTTGGAAGCATCCCGATTCAAGGGCTCTCTAATCTTTTCACCGTGTACGGTGAGGTATTCGCTACAACTGATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATATCTTGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGACAGCCACGACAATTGCAGTATTCGGTGTTAGAGACCCACAAAATGCAGTGGTGCCAATTTGGTACCACAGTGGAAAGATCGCGGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTCACAGCAACGATCAAGCTTGGCTGTTCGACTTGGACGGCGGCATTCTGTATAGCCAGCCGCTCGTGGATCGCCAAACTGTTGATACAATCTTTAGTTTGGACGGGAAGCTTATTGCGCCAGACCTTATTCCACAAGTACAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGTTTGCATGTGACTACCATAGACGGTGTCGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAGGCCTGGCAGATATCTCGTCAGAACCGATTGGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCGTCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAACACATCCCCTCGACGCCGTAGCATCGTTCCCGAGGAAATAACGATTCCAACGCTGGCTGGCATCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATAGACCGAGACAATTGGAAGCACTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTACTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTGCCAGATAGACATCAAAGTAGGCGATGCTGAGACTATGCTTGCATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGAGGATCTGTGGGCCATTCTACACATGACATAG
本发明提供由所述的抗虫性的苏铁毒蛋白基因优化得到的苏铁毒蛋白优化基因，所述苏铁毒蛋白优化基因的核苷酸序列如下：
SEQ ID NO.6：
atggtgatggttattgattacccttctttgagggcttctttgtatgctactaagtctgctgttccttgtaggcctcttcctggtaaccctgaaagggtttctaggagggtttctagaaggggtgctggtgaaattggtagtcatgctgatttttggatttctagagctaaggcttctgctattaggatttttcctagacaagaaggtccattgagggaaagcaggaggaggttctatttgttgagagaggctgttttcacaggtaggaggactgcatgggctgcaaatcctgctgctcagagagcagcttgggatagaaagaggtctcctttcttcggtaggtcatcagatgatattcattcattcagtaagaacccagaagagttccttactcaaaatactttgtcttgttctcttcttactagaactgctcaaactgttactaagtctcctgttttgcctgctcaaggttgggttaaggttggtagagtgccaggtagaggtgatgctcttttcgttgagcttgttgctgaaaaatctgatgaaactgttaaggcttacttgttgggttataatggtaagaatgattctcctgaacctgcttatgttgatattccaaagaggggtgcagagaatggaactttcctttttacagtggatcttagcggttgttctgttgttgttactcaattggatgagcagacttatagagtgttccatgataagagggttaattcaagtattctttacgataacgtggttatgtatgttgattttaaccattacaggtaccctagagctcatggtcatggtagagatactggtcaagctgttgtttgtatgcagtttgctgatggtgtttggaagttgttcttgcaaagacatgagactgctaattctcttccaaagatttggtctagaggtgatggtaagcctgaaattttctctgatgctgagcctagacctcatatggaggatggtattaaggagttccttaatagaaggagggctttgcaagatgaattgaagcattttgctaatactttggaagttgatcctgatattgtttctaatgctgttgatggtgaatatgaaggtggtcaattttctagaaatgatgatgcaattaggggttggaatgaattgagagcttctatgagacagaagatggctgaaagaaagaagcttcttcaaactaagaagggtatgttggatagtttcaataatccagatcatcctctttacaagttcagaaaacaagttttggatgaattgaaagagatgtcagcttatgataatacacttgaagcttctgaaatcgctgataggacttggctttggcttgaaattaagaaggctaagggaacaaatgcagttgttaatagcggagctgcacctaagcaaccatcccttactgttggtgaaagatatgctgaaatggaagcagcactttggatgagaagcgatacagctgctttcgaacaaggatatgaaagttatcaaactgttagagttccaggttataatgaagatatgacaagcattgctatgaagaagttgtttgttgaatcttccttgacagatgaacaacttggtgctctttgtaagaggatttccattgctagtgatcaagaaaatcatttgttgactcaaaagacccaagaaatcgaatcaactttcaagcaattctccgatggaagcttccttgaatctgctccacaagatttcttccttagaagagttggtgataaatccggtggtaggtgctacccacttgttagggttatggctgttgctctttctgaaggtgaagatgttgctcaaaatttcatggaaaatttgaggactttgtcaaggaatcctgaatattccggtgaatcagcttcttctcaagctacaaatgctggtcttttcagagatgctcttgctggtcttcatgcaaacgttgaagctatcgctgcatccgagaagcttcctgctgtttttggtttgcctgatgttgctactatgctttctcaggcttctgaatctaaggtttatgctcttaatactgaaactcattctatgcttgttgctgttattgttgataagcaatctttggctaggaagtattatttttacgatcctaatttccttatggctggtttctctgatattactagattctccgaagctcttaagtaccatttcgttaatttgcagtttgcaggtaattataaggctttcggtactgctgaagctcctcaattcaatcttgttcttattgatactaccgctatggaggatgctcatgttgatggtggtattactgtgaagcaactttctgaaggagttgatcttactaatgttttcgattctactaagaaggctagagtggttactgaggctgcagctaggttggctgaagatcagcaattgagaactggacttacaattattgaagctgatcagtgggctaatagatggcaggatgctgttattaggttgaagcttaaggaaggtctttctggtgagtggatgcctttgcttgaaaagatgcaagaaaatgcagatggtagttatcagattgatttcattaacactaagaacactggagaaattaggggagtttcaactagggatgctacttttaaggaattcaagtcttatttggataagaaccttgatgtgttgcataaggattttattttccaggatggagtgttcaaacctcttgagggtgagcctattgatggacttaatgctatgttcgctgttcaaatgattattgattggttcaataggggtactaacgcagaagaagatgctgcttctggttctactaacgctcaattgtctttggctattaaggttcattcttatatcaacatggcacaagtggcacatactgctgttactgatgtggcaaaggtttataatttggtgaaatctgctctttacggtgaacaagttgctggtgaaacatcattgttcactttcggtaaggctcttaaatttgctgctactgaaggtcttggtgtgttgcttggtgcttcttttgtgatccttgatgcttatcagctttctcttgttgagaatgatgttcagaaggcaattattggtactcaacttgcttttgattctgcttcttttcttgctggtattggtgctattgttgctggtgcttctggtgttggtatgattttgggtggtgctggtgttgttttgggtggtttgggtgttggttttggtgctttggctcaggcttttggtgagattactgctgatgctcagttggttggtaagtattttgctttgttggatgaggcttataggggtggtggttataggtatgagtctaaggataaggctttgatgcctttggctggtgctgtgattgataagattagacttgctgataagcatttgcaagttcattttgattctcaattcatctacaggactcatcatggttctactggttctggtaagattaattatttcttctgggctggtgatatgcctcaaatggttcatgatagatctcaagctattaatgttagggagcagattggttataagaatgataatgctactcttgaacttccagaggatgcttctgatcttattcttcctgcaactcctaagtctttcattacatatagatacgagatcttgccattcgctacttctagacatgatactggttttgatgttattagaagacttgaggaggattatagattcgattatgatttctacatcttcccttctgaatataccattagaactattaagcacgaatacgttgaaaccaaggttgctgttgtgcttgatagaagatctttgagacttggtgctcctgagcttttgaaggaacaacatggttatcttatttacgagattgaaggtacaggtggtcaatatgttatttgtttgaatcagggttctactgtgactcttaagacaactggttctgaagcaaataagactctttggattttggatgctaggcaacttattgctggtaatgatagtatcactgtttctcaagattctattactatcggttctactactgtgcatcttaggaatcaggatttcgattgtcttcagattgttatgtctagagaagaacttcttcaagttgatttggcttctggtgaagctagagttttctccgaagatgcttctaagtggcttgatacaaaggaaactattcatgaacaccttcacaagttggctgaagaacataggcttcatggtcaattcgttgttgttaataattactacaggggtgttggtagagcttattatgatgctgctcatgatagaatgctttatactgatgttgaggatgaaactcttacaagagatgctgttcttgctggtgttactggtgaagatgcttatttttataacgttaagcagaacgctttgtggagagttaacgctggtactcaccaagttattactaagtattatcctactcttcttgaggatcctgattctaacatggctagagttgagcaacatggtaatatcgttgttatggctatgtcaagattgaatcatcctgataagggtgttaacggtattatttatcttgttcatcaggaatctatcgttcttactgctgttattggaaactctgaaatgcttaagagacttagacaaacttctactatctctcttactgaacttaggggtgttaacgatatggctcttgtttcaactatggataggcttcctggtcagaaggtggtggctacaaatgcagaattcgttacacttttggctaaggatgaacaaggagctaatattaaggaacaaactgtttattgccagcaaggtactggtcaaactaatgatacttctattgaatcaatccctatccctggtttgtctaatcttcttacagtttatggtgaggttttcgctacaactaatgatggtagaatccttagattgcttggtgatggaagcacttgtcttgagggtatcaatgaacattggttgattaagcatcctaaatggtggcaggatcttgctactcttgaggcagagaaggctaccactgttgctgtttttggagttagggatttgcaaaatgctgttgttcctatttggtatcataacggtaagattgcagttgcttcatctaaactttatggtaagcaattgcagtttttgggttttaattctaacggagaagcttggcttttcgatcttgatgatggtattttgtattctcaacctttggttgattctttcctttatgctattttcagtttggatggtaagttgattgctcctatctttattcctcaggttgaacaaatgttgtccggtcaacatcttaggaaccctgttattgttgatggtaggcttcatgttactactattgatggtgttgttgttggtttcgataagaagtctcctactcttgtggctgttaataaggcttggcaaatttctagacaaaataggcttgaggaggctttgggtaatttgttgtctaattggtctgatcataacgatgctattgttcttcagggtgatcctgagtctgctcctgcttggtattatgttccttctagggagattgtgactgctaatggtttgacttgggctgatcaccctctttggcttggaatggctgttggtgctgctactccttatatttattctacttctagaaggagggtttatgttgttgagtctaagaatatggctaaggataagggtgaatttgctgctgctcattgttttgctaagtctttggttcttcagtctattgttcctgaggaaattactattccaactcttgctggtatcgatttcgctttggttgctgctactaatgctactattgttattgatagggataactggaagcattataaggcaattgttcttgataacaaggttgatcaagatggaggtgctaaggttgtggttggttctgagattcctatgaaggttcttgtgaggaagtttcaaggtaatttggaatttttcgatcctaataacggtaatgtggttattcttcaaaacgttcttaagggagatggtgcttgttgtcagattgagattaaggttggtgatgcagagactatgttggtttctgatttctctgagattttctctaagactcatcctttggttgcttctcaggttttcgaacttggtgatgttattgctgttactaagcataagagtgattatgctgatctttgggctattttgaggatgacttga
SEQ ID NO.7：
ATGGAAGAAGCTGATATGGAAAATAGAGGATACGAAGAAAGACATAGCCAAAGCCAAAGCGCTACTCCTCTTAGAAGAAGAAAGAGAAGCCCTTTCTTCGGAAGAAGCAGCGATGATATTCATAGCTTCTCAAAGAATCCTGAAGAATTCCTTACAAAAAATACTCTTTCTTGTTCTCTTCTTACTAGAACTGCTCAAACTGTTACTAAGTCTCCTGTTCTTCCTGCTCAAGGTTGGGTTAAGGTTGGTAGAGTTCCTGGTAGAGGTGATGCTCTTTCTGGTTGTTCTGTTGTTGTTACTCAACTTGATGAACAAACTTATAGAGTTTTCCATGATAAGAGAGTTAATTCTTCTATTCTTTATGATAATGTTGTTATGTATGTTGATTTCAATCATTATAGATATCCTAGAGCTCATGGTCATGGTAGAGATACTGGTCAAGCTGTTGTTTGTATGCAATTCGCTGATGGTGTTTGGAAGCTTTTCCTTCAAAGACATGAAACTGCTAATTCTCTTCCTAAGATTTGGTCTAGAGGTGATGGTAAGCCTGAAATTTTCTCTGATGCTGAACCTAGACCTCATATGGAAGATGGTATTAAGGAATTCCTTAATAGAAGAAGAGCTCTTCAAGATGAACTTAAGCATTTCGCTAATACTCTTGAAGTTGATCCTGAAATTGTTTCTAATGCTGTTGATGGTGAATATGAAGGTGGTCAATTCTCTAGAAATGATGATGCTATTAGAGGTTGGAATGAACTTAGAGCTTCTATGAGACAAAAGATGGCTGAAAGAAAGAAGAGACTTGAAAGAAATAAGGGTATGCTTGATTCTTTCAATAATCCTGATCATCCTCTTTATAAGTTCAGAAAGCAAGTTCTTGATGAACTTAAGGAAATGTCTGCTTATGATAATACTCTTGAAGCTTCTGAAATTGCTGATAGAACTTGGCTTTGGCTTGAAATTAAGAAGGCTAAGGGTACTAATGCTGTTGTTAATTCTGGTGCTGCTCCTAAGCAACCTTCTCTTACTGTTGGTGAAAGATATGCTGAAATGGAAGCTGCTCTTTGGATGAGATCTGATACTGCTGCTTTCGAACAAGGTTATGAATCTTATCAAACTCTTAGAGTTCCTGGTTATAATGAAGATATGACTTCTATTGCTATGAAGAAGCTTTTCGTTGAATCTTCTCTTACTGATGAACAACTTGGTGCTCTTTGTAAGAGAATTTCTATTGCTTCTGATCAAGAAAATCATCTTCTTACTCAAAAGACTCAAGAAATTGAATCTACTTTCAAGCAATTCTCTGATGGTTCTTTCCTTGAATCTGCTCCTCAAGATTTCTTCCTTAGAAGAGTTGGTGATAAGTCTGGTGGTAGATGTTATCCTCTTGTTAGAGTTATGGCTGTTGCTCTTTCTCAAGGTGAAGATGTTGCTCAAAATTTCATGGAAAATCTTAGAACTCTTTCTAGAAATCCTGAATATTCTGGTGAATCTGCTTCTTCTCAAGCTACTAATGCTGGTCTTTTCAGAGATGCTCTTGCTGGTCTTCATGCTAATGTTGAAGCTATTGCTGCTTCTGAAAAGCTTCCTGCTGTTTTCGGTCTTCCTGATGTTGCTACTATGCTTTCTCAAGCTTCTGAATCTAAGGTTTATGCTCTTAATACTGAAACTCATTCTATGCTTGTTGCTGTTATTGTTGATAAGCAATCTCTTGCTAGAAAGTATTATTTCTATGATCCTAATTTCCTTATTGCTGGTTTCTCTGATATTACTAGATTCTCTGAAGCTCTTAAGTATCATTTCGTTAATCTTCAATTCGCTGGTAATTATAAGGCTTTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACTGCTATGGCTGATGCTCATGTTGATGGTGGTATTACTGTTAAGCAACTTTCTGAAGGTGTTGATCTTACTAATGTTTTCGATTCTACTAAGAAGGCTAGAGTTGTTACTGAAACTGCTGCTAGACTTGCTGAAGATCAACAACTTAGAACTGGTCTTACTATTATTGAAGCTGATCAATGGGCTAATAGATGGCAAGATGCTGTTATTAGACTTAAGCTTAAGGAAGGTCTTTCTGGTGAATGGATGCCTCTTCTTGAAAAGATGCAAGAAAATGCTGATGGTTCTTATCAAATTGATTTCATTAATACTAAGAATACTGGTGAAATTAGAGGTGTTTCTACTAGAGATGCTACTTTCAAGGAATTCAAGTCTTATCTTGATAAGAATCTTGATGTTCTTCATAAGGATTTCATTTTCCAAGATGGTGTTTTCAAGCCTCTTGAAGGTGAACCTATTGATGGTCTTAATGCTATGTTCGCTGTTCAAATGATTATTGATTGGTTCAATAGAGGTACTAATGCTGAAGAAGATGCTGCTTCTGGTTCTACTAATGCTCAACTTTCTCTTGCTATTAAGGTTCATTCTTATATTAATATGGCTCAAGTTGCTCATACTGCTGTTACTGATGTTGCTAAGGTTTATAATCTTGTTAAGTCTGCTCTTTATGGTGAACAAGTTGCTGGTGAAGCTTCTCTTCTTACTTTCGGTAAGGCTCTTAAGTTCGCTGCTACTGAAGGTCTTGGTGTTCTTCTTGGTGCTTCTTTCGTTATTCTTGATGCTTATCAACTTTCTCTTGTTGAAAATGATGTTCAAAAGGCTATTATTGGTACTCAACTTGCTTTCGATTCTGCTTCTTTCCTTGCTGGTATTGGTGCTATTGTTGCTGGTGCTTCTGGTGTTGGTATGATTCTTGGTGGTGCTGGTGTTGTTCTTGGTGGTCTTGGTGTTGGTTTCGGTGCTCTTGCTCAAGCTTTCGGTGAAATTACTGCTGATGCTCAACTTGTTGGTAAGTATTTCGCTCTTCTTGATGAAGCTTATAGAGGTGGTGGTTATAGATATGAATCTAAGGATAAGGCTCTTATGCCTCTTGCTGGTGCTGTTATTGATAAGATTAGACTTGCTGATAAGCATCTTCAAGTTCATTTCGATTCTCAATTCATTTATAGAACTCATCATGGTCTTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGCTGGTGATATGCCTCAAATGGTTCATGATAGATCTCAAGCTATTAATATTAGAGAACAAATTGGTTATAAGAATGATCATGCTACTCTTGAACTTCCTGAAGATGCTTCTGATCTTATTCTTCCTGCTACTCCTAAGTCTTTCATTACTTATCAATATCAAATTCTTCCTTTCGCTACTTCTAGACATGATAGAGGTTTCGATGTTATTAGAAGACTTGAAGAAGATTATAGATTCGATTATGATTTCTATATTTTCCCTTCTGAATATACTATTAGAACTATTAAGCATGAATATGTTGAAACTAAGGTTGCTGTTGTTCTTGATAGAAGATCTCTTAGACTTGGTGCTCCTGAACTTCTTAAGGAACAACATGGTTATCTTATTTATGAAATTGAAGGTACTGGTGGTCAATATGTTATTTGTCTTAATCAAGGTTCTACTGTTACTCTTAAGACTACTGGTTCTGAAGCTAATAAGACTCTTTGGATTCTTGATGCTAGACAACTTATTGCTGGTAATGATTCTATTACTGTTTCTCAAGATTCTATTACTATTGGTTCTACTACTGTTCATCTTAGAAATCAAGATTTCGATTGTCTTCAAATTGTTATGTCTAGAGAAGAACTTCTTCAAGTTGATCTTGCTTCTGGTGAAGCTAGAGTTTTCTCTGAAGATGCTTCTAAGTGGCTTGATACTAAGGAAACTATTCATGAACATCTTCATAAGCTTGCTGAAGAACATAGACTTCATGGTCAATTCGTTGTTGTTAATAATTATTATAGAGGTGTTGGTAGAGCTTATTATGATGCTGCTCATGATAGAATGCTTTATACTGATGTTGAAGATGAAACTCTTACTAGAGATGCTGTTCTTGCTGGTGTTACTGGTGAAGATGCTTATTTCTATAATGTTAAGCAAAATGCTCTTTGGAGAGTTAATGCTGGTACTCATCAAGTTATTACTAAGTATTATCCTACTCTTCTTGAAGATCCTGATTCTAAGATGGCTAGAGTTGAACAACATGGTAATATTGTTGTTATGGCTATGTCTAGACTTAATCATCCTGATAAGGGTGTTAATGGTATTATTTATCTTGTTCATCAAGAATCTATGGTTCTTACTGCTGTTATTGGTAATTCTGAAATGCTTAAGAGACTTAGACAAACTTCTACTATTTCTCTTACTGAACTTAGAGGTGTTAATGATATGGCTCTTGTTTCTACTATGGATAAGCTTCCTGGTCAAAAGGTTGTTGCTACTAATGCTGAATTCGTTACTATTCTTGCTAAGGATGAACAAGGTTCTCAAGTTTGTTTCTGGCTTAGAACTGTTGATGGTGTTCTTATTAAGCCTTCTATTCAACCTCCTATTCCTACTGATCTTACTCTTACTGTTTCTTCTCATAAGGTTGCTGGTCAAGATGTTTTCTATTTCTATTCTATTAAGGAACAAACTGTTTATTGTCAACAAGGTACTGGTCAAACTAATGATACTTCTATTGAATCTATTCCTATTCCTGGTCTTTCTAATCTTCTTACTGTTTATGGTGAAGTTTTCGCTACTACTGATGATGGTAGAATTCTTAGACTTCTTGGTGATGGTTCTACTTATCTTGATGGTATTAATGAACATTGGCTTATTAAGCATCCTAAGTGGTGGCAAGATCTTGCTACTCTTGAAGCTGAAAAGGCTACTACTGTTGCTGTTTTCGGTGTTAGAGATCTTCAAAATGCTGTTGTTCCTATTTGGTATCATAATGGTAAGATTGCTGTTGCTTCTTCTAAGCTTTATGGTAAGCAACTTCAATTCCTTGGTTTCAATTCTAATGGTGAAGCTTGGCTTTTCGATCTTGATGATGGTATTCTTTATTCTCAACCTCTTGTTGATTCTTTCCTTTATGCTATTTTCTCTCTTGATGGTAAGCTTATTGCTCCTATTTTCATTCCTCAAGTTGAACAAATGCTTTCTGGTCAACATCTTAGAAATCCTGTTATTGTTGATGGTAGACTTCATGTTACTACTATTGATGGTGTTGTTGTTGGTTTCGATAAGAAGTCTCCTACTCTTGTTGCTGTTAATAAGGCTTGGCAAATTTCTAGACAAAATAGACTTGAAGAAGCTCTTGGTAATCTTCTTTCTAATTGGTCTGATCATAATGATGCTATTGTTCTTCAAGGTGATCCTGAATCTGCTCCTGCTTGGTATTATGTTCCTTCTAGAGAAATTGTTACTGCTAATGGTCTTACTTGGGCTGATCATCCTCTTTGGCTTGGTATGGCTGTTGGTGCTGCTACTCCTTATATTTATTCTACTTCTAGAAGAAGAGTTTATGCTGTTGAATCTAAGCATATGGCTAAGGATAAGGGTGAATTCGCTGCTGCTCATTGTTTCGCTAAGTCTCTTGTTCTTCAATCTATTGTTCCTGAAGAAATTACTATTCCTACTCTTGCTGGTATTGATTTCGCTCTTGTTGCTGCTACTAATGCTACTATTGTTATTCATAGAGATAATTGGAAGCATTATAAGGCTATTGTTCTTGATAATAAGGTTGATCAAGATGGTGGTGCTAAGGTTGTTGTTGGTTCTGGTATTCCTATGAAGGTTCTTGTTAGAAAGTTCCAAGGTAATCTTGAATTCTTCGATCCTAATAATGGTAATGTTGTTATTCTTAGAAATGTTCTTAAGGGTGATGGTGCTTGTTCTCAAATTGAAATTAAGGTTGGTGATGCTGAAACTATGCTTGTTTCTGATTTCTCTGAAATTTTCTCTAAGACTCATCCTCTTGTTGCTTCTCAAGTTTTCGAACTTGGTGATGTTATTGCTGTTACTAAGCATAAGTCTGATTATGCTGATCTTTGGGCTATTCTTAGAATGACTTGA
SEQ ID NO.8：
ATGGCAGTTGCTTTGAGCCAAGGTGAAGATGTTGCTCAGAATTTCATGGAGAATTTGAGGACTTTGAGCAGGAATCCTGAATATAGCGGTGAAAGCGCTTCCTCTCAGGCTACCAATGCTGGTCTTTTTAGGGATGCTTTGGCTGGTCTTCATGCTAACGTTGAAGCTATCGCTGCTTCTGAGAAGTTGCCTGCTGTGTTCGGATTGCCTGATGTTGCTACTATGCTTTCTCAAGCTTCTGAGTCTAAGGTTTATGCTCTTAATACTGAAACTCATTCTATGCTTGCTGCTGTTATCGTTGATAAGCAATCTTTGGCTAGGAAGTATTATTTCTATGATCCTAATTTCCTTATGGCTGGTTTCTCTGATATTACTAGATTCTCCGAAGCTCTTAAGTATCATTTTGTTAATCTTCAGTTCGCTGGTAATTATAAAGCTTTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACCGCTATGGCTGATGCTCATGTTGATGGTGGTATTACTGTGAAACAACTTTCTGAGGGTGTGGATTTGACTAATGTTTTTGATTCTACTAAGAAGGCTAGAGTGGTTACCGAGGCAGCAGCTAGGTTGGCTGAAGATCAGCAATTGAGAACTGGATTGACTATTATTGAAGCAGATCAATGGGCTAATAGATGGCAGGATGCTGTGATTAGATTGAAGCTTAAGGAAGGACTTTCTGGTGAGTGGATGCCTCTTCTTGAAAGAATGCAAGAAAATGGACACGGTAGCTATCAAATTGAATTTCTTAATACCAAGAACACCGGTGAAACAAGATGGGTTAGCACAAGAGATGCTACTTTTAAGGAATTCAAGTCTTATCTTGATAAGAACTTCGATGCTTTGAGGAAGGATTTCATTTTCCAAGATGGAGTTTTCAAACCTCTTGATGGTGAGCCTATTGATGGTCTTAATGCTATGTTCGCTGTTCAAATGATTATTGATTGGTTCAATCCTGGTACTAATGCTGAGGAGGATGCTGCTAGTGGTTCTACTAATGCTCAACTTTCTTTGGCTATTAAGGTGCATTCTTATATTAACATGGCTCAAGTTGCTCACACTGCTGTTACTGATGTTGCAAAGGTTTATAATCTTGTTAAGTCTGCTCTTTACGGTGAACAAGTTGCTGGTGAAGCTAGCTTGCTTACTTTCGGTAAGGCTCTTAAGTTCGCTGCTACTGAAGGTTTGGGTGTTCTTTTGGGTGCTTCTTTCGTTATTCTTGATGCTTATCAGCTTTCTCTTGTTGAGAATGATGTTCAGAAGGCAATTATTGGAACTCAATTGGCTTTTGATTCTGCTTCTTTTTTGGCTGGTATTGGTGCTATTGTTGCTGGTGCTTCTGGTGTTGGTATGATTTTGGGTGGTGCTGGTGTTATTTTGGGTGGATTGGGTGTTGGTTTTGGTGCTTTGGCTCAGGCTTTTGGTCAGATTACTGCTGATGCTCAGTTGGTTGGTGAGTATTTTGCTTTGTTGGATGAGGCTTATTCTGGTGGTGGTTATAGGTATGAGTCTAAGGATAAGGCTTTGATGCCTCTTGCTGGTGCTGTGATTGATAAGATTAGGCTTGCTGATAAGAGGTTGCAAGTTCATTTTGATTCTCAATTTATCTACAGGACTCATCACGGTTCTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGTTGGTGATATGCCTCAAATGGTTCATGATAGATCTCAAGCTATTAATGTTAGGGAACAGATTGGTTATAAGAATGATTCTGCTACTTTGGAATTGCCTGAAGATGCTTCTGATTTGATTTTGCCTGCTACTCCTAAGTCTTTCATTACATATAGATACGAAATCCTTCCATTCGCTACATCTAGACATGATAGAGGTTTTGATGTTATTAGAAGATTGGAAGAAGATTACAGGTTCGATTATGATTTCTACATTTTCCCTTCCGAGTACACTATTAGAACTATTAAGCATGAATACGTGGAAACTAAGGTTGCTGTTGTTCTTGATAGAAGATCTTTGAGACTTGGTGCTCCTGAACTTTTGAAGGAGCAGCATGGTTATTTGATTTACGAGATTGAAGGAACTGGTGGTCAATATGTTATTTGTTTGAATCATGGTTCCACTGTTACTCTTAAGACTACTGGTTCCGAAGCTAATAAGACACTTTGGATTTTGGATGCTAGGCAGCTTATTGCTGGAAATGATTCTATCACTGTTTCTCAAGATTCTATTACTATCGGTTCTACTACTGTTCACCTTAGAAATCAAGATTTCGATTGCCTTCAAATTGTGATGTCTAGGGATGAACTTCTTCAAGTGGATCTTGCTTCTAGGGAAGTTAGGGTTTTCTCCGAAGATGCTTCAAAATGGCTTGATACCAAGGAAACTATTCATGAACATCTTCACAAGTTGGCTGGTGAGCATAGACTTCATGGTCAATTCGTTGTTGTTAATAATTACTACAGGGGTGTGGGTAGAGCTTACTATGATGCTGCACATGATAGAATGCTTTATACTGATGTTGAAGATGAAACTCTTACAAGAGATGCAGTTCTTGCTGGTGTTACTGGAGAAGATGCTTATTTTTATAACGTTAACCAGAACGCTCTTTGGAGGGTTAACGCTGATACTCATCAAGTTATTACTAAGTATTACCCTACCTTGTTGGAGGATCCTGATTCTAAAATGGCTAGAGTTGAGCAACATGGAAATATCGTTGTTATGGCTATGTCAAGATTGAATCATCCTGATAAGGGTGTTAACGGTATTATTTATCTTGTTCATCAGGAATCTATGGTTCTTACTGCTGTTATTGGTAATTCTGAAATGCTTAAGAGGTTGAGACAAACTTCTACTATCTCTCTTACTGAACTTAGGGGTGTTAACGATATGGCTCTTGTTTCTACTATGGATAGACTTCCTGGTCAAAAGGTGGTTGCTACTAATGCTGAATTCGTGACACTTCTTGCTAAGGATGAACAAGGTTCTCAAGTGTGTTTTTGGTTGAGGACTGTTGATGGTGTTTTGATTAAGCCTTCTATTCAGCCTCCTATTCCTACTGATCTTACTCTTACTGTTTCTTCTCATAAGGTTGCTGGTCAGGATGTTTTTTATTTTTATTCTATCAAGGAGCAGACCGTTTATTGTCAACAAGGTACCGGACAAACCAACGATACCTCTATTGAATCTATTCCTATTCCTGGTTTGTCTAATCTTCTTACTGTTTATGGTGAGGTTTTTGCTACTACTAATGATGGTAGAATTCTTAGACTTTTGGGTGATGGAAGCACTTATCTTGAAGGTATTAATGAGCATTGGTTGATTAAGCATCCTAAGTGGTGGCAGGATTTGGCTACTTTGGAGGCAGAGAAGGCTACAACCGTTGCAGTTTTTGGTGTGAGGGATCCTCAAAATGCTGTGGTGCCTATTTGGTATCATAACGGAAAAATTGCTGTTGCTTCAAGCAAGCTTTATGGTAAGCAATTGCAATTCCTTGGATTTAATTCTAACGGTGAAGCATGGCTTTTCGATCTTGATGATGGTATTTTGTATTCTCAACCTTTGGTTGATTCTCAAGCTGTTGATGCTATTTTTTCTCTTGATGGTAAGCTTATTGCTCCAAATTTGATTCCTCAAGTTGAACAGATGTTGTCTGGTCAACACCTTAGGAATCCTGTTATTGTGGATGGTAGGTTGCATGTTACTACTATTGATGGTGTTGTTGTTGGTTTCGATAAGAAGTCTCCTACTCTTGTGGCTGTTAATAAGGCTTGGCAAATTTCTAGACAAAATAGGTTGGAAGAGGCTTTGGGTAATTTGTTGTCTAATTGGTCTGATCATAACGATGCTATTGTTCTTCAGGGTGATCCTGAGTCTGCTCCTGCTTGGTATTATGTTCCTTCTAGGGAGATTGTGACTGCTAATGGTTTGACTTGGGCTGATCACCCTCTTTGGCTTGGAATGGCTGTTGGTGCTGCTACTTTGTATATTTATTCTACTTCTAGGAGGAGAGTTTATGCTGTTGAGTCTAAGAAGATGGCTAAGGATAAAGGTGAATTTGCTGCTGCTCATTGTTTTGCTAAGTCTCTTGTTCTTCAATCTATTGTTCCTGAAGAAATTACAATCCCAACTTTGGCTGGTACTGATTTCGCTTTGGTTGCTGCTACTAATGCAACTATTGTTATTGATAGAGATAACTGGAAGCATTATAAGGCTATTGTGTTGGATAATAAGGTTGATCAAGATGGAGGTGCTAAGGTTGTGGTTGGTTCTGAGATTCCTATGAAGGTTTTGGTTAGAAAGTTTCAAGGTAACTTGGAATTCTTCGATCCTAATAATGGTAATGTTGTTATCCTTCAAAACGTTCTTAAGGGAGATGGTGCTTGTTCTAAGATTGAAATTAAAGTTGGTGATGCTGAAACTATGCTTGTTAGTGATTTCTCTGAGATTTTCTCTAAGACTCATCCTTTGGTTGCTTCTCAGGTTTTCGAACTTGGTGATGTTATTGCAGTTACTAAGCATAAGTCTGATTATGCTGATCTTTGGGCTATTTTGAGGATGACTTGA
SEQ ID NO.9：
ATGGAAGAAGTTGATATGCAAAACAGAGGATACGAAGAATGGCACAGCCAAAGCCAAAGCGCTACTACTTTGAGAAGAAGAAAGAGAAGCCCTGTGTTCGGTAGGAGCAGCGATGATATTCATAGCTTCAGCAAGAATCCAGAAGAATTCCTTACTCAAAACACACTTTCATGTTCACTTCTTACTAGGACAGCTGAGACAGTTACCAAGTCTCCTGTTCTTCCAGAAAGAGGATGGGTTAAAGTGGGTAGAGTTCCAGGTAGAGGTGATGCTCTTTTCGTTCAAGTTGTTGCTGAACAATCATATGATACTGTGAAGGCTTATTTGCTTGGTTATAATGGTGAGAATGATTCTCCTAATCCTGCTTATGTTGATATTCCTAAGAGGGCTGCTGAGAATGGTACTTTCCTTTTTACTGTTGATTTGTCTGGTTGTTCTGTTGTTGTTACTCAGTTGGATGAGCAAACTTATAGAGTGTTTCATGATAAGAGAGTTAACTCATCAATCCTTTATGATAACGTTGTTATGTACGTTGATTTCAATCATTACAGGTATCCTAGGGCTCACGGTCATGGTAGAGATACTGGTCAAGCTGTTGTTTGTATGCAATTTGCTGATGGTGTTTGGAAGCTTTTCCTTCAAAGACATGAGACTGCTAATTCTCTTCCTAAGATTTGGTCTAGAGGTGATGGTAAACCTGAAATTTTCTCTGATGCTGAGCCTAGACAACATATGGAGGATGGTATCAAGGAGTTCCTTAATAGAAGGAGAGCTTTGCAAGATGAATTGAAGAAGTTCGCAGATATGTTCGGAGTTGATCCTGAGATTGTTTCTAATGCTGTGGATGGTAAGTATAAGGGTGGTCAATTTTCTAGAAATGATGATGCAATTAGGGGTTGGAATGAATTGAGAGCTTCAATGAGGCAAAAGATGGCTGAAAGAAACAAGGTGTTGCTTACTATGAAGTTGGAACAACCTAGAAGCAGCAGGGTTTTGCCTTTGGTGAATGCTACTCAAAGATGGAGGAGGCCTTTCGGTTGTGTTGCAACTCAAAGAAGACTTTCTAAGGCTGGTAGATTGGTTCAGGCTTATATTCATAGGGATCAGGAGAATCATTTGTTGACTCAAAAGACTCAAGAGATTGAGTCCACTTTCAAGCAATTCTCAGATGGTTCATTTCTTGAATCTGCTCCACAAGATTTCTTCCTTAGGAGAGTGGGTGATAAGTCTGGTGGTAGGTGTTACCCACTTGTTAGAGTTATGGCTGTTGCTCTTTCTCAGGGTGAAGATGTTGCTCAAAATTTCATGGAAAACCTTAGGACTCTTTCAAAGAATCCTGAGTATTCTGGTGAATCTGCTTCTTCTCAAGCTACAAATGCTGGTCTTTTCAGAGATGCTCTTGCTGGTCTTCATGCTAACGTTGAAGCTATTGCTGCTTCTGAAAAGTTGCCTGCTGTGTTCGGTTTGCCTGATGTTGCTACTATGCTTTCCCAAGCTTCTGAATCTAAGGTTTATGCTCTTAATACTGAAACTCATTCTATGCTTGCTGCTGTTATTGTTGATAAGCAATCTTTGGCTAGGAAGTATTATTTTTACGATCCTAATTTCCTTATGGCTGGTTTCTCTGATATTACTAGATTCTCCGAAGCTCTTAAGTACCATTTTGTTAATCTTCAGTTCGCAGGTAATTATAAGCCATTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACCGTTATGGCTGATGCACATGTTGATGGTGGTATTACTGTTAAGCAACTTTCTGAGGGTGTGGATCTTACTAATGTTTTCGATTCTACTAAGAAGGCTAGAGTTGTTACTGAAGCAGCTGCTAGGTTGGCTGAAGATCAGCAATTGAGAACTGGACTTACCATTATTGAAGCTGATCAATGGGCTAATAGATGGCAGGATGCTGTTATTAGACTTAAGCTTAAGGAAGGACTTTCTGGTGAGTGGATGCCTTTGCTTGAAAAGATGCAAGAAAATGCAGATGGTAGTTATCAAATCGATTTCATCAACACTAAGAACACTGGTGAGATTAGAGGTGTTTCAACTAGAGATGGAACTTTCAAGGAACTTAAGTCTTATTTGGATAAGAACTTGGATGCTTTGCACAAAGATTTCATTTTTCAAGATGGTGTTTTCAAGCCTTTGGAGGGTGAGCCTATTGATGGTCTTAATGCAATGTTCGCTGTTCAAATGATTATTGATTGGTTCAATAGGGGTACTAATGCTGAAGAGGATGCTGCTTCTGGTAGTACTAACGCTCAGTTGTCTTTGGCTATTAAGGTTCATTCTTATATCAACATGGCTCAAGTTGCTCATACTGCTGTTACTGATGTTGCTAAGGTTTATTCACTTGTTAAGTCTGCTTTGTACGGTGAACAAGTTGCTGGTGGTGCTTCATTGCTTACTTTCGGTAAGGCTTTGAAGTTGGCTGCTACTGAAGGTCTTGGTGTTCTTTTGGGTGCTCCTGTTGTTATTCTTGATGCTTATCAGCTTTCTCTTGTTGAAAATGATGTTCAGAAGGCTATTATTGGTACTCAGTTGGATTTCGATTCTGCTTCTTTTCTTGTTTCTATTGGTGCTGCTGTTGCTGGTGTTTCTGGTGTTGGTATTGTTCTTTCTGGTGCTGGTGTTTTGTTGGCTGGTTTGGGTGTTGGTTTTGGTGCTTTGGCTCAGGCTTTTGGTGAGATTACTGCTGATGCTCAGTTGGTTGGTAAGTATTTTGCTTTGTTGGATGAGGCTTATAGGGGTGGTGGTTATAGGTATGAGTCTAAGGATAAGGCTTTGATGCCTTTGGCTGGTGCTGTGATTGATAAGATTAGACTTGCTGATAAGCATTTGCAAGTTCATTTTGATTCTCAATTCATCTACAGGACTCATCATGGTTCTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGTTGGTGATATGCCTAAGATGGTTCATGATAGATCTCAAGCTATTAATGTTAGGGAACAGATTGGTTATAAGAATGATTCTGCTACTTTGGAATTGCCTGAAGATGCTTCTGATTTGATTTTGCCTGCTACTCCTAAGTCTTTCATTACATATAGATACGAAATCCTTCCATTCGCTACATCTAGACATGATAGAGGTTTTGATGTTATTAGAAGATTGGAAGAAGATCATAGATTCGATTATGTTTTCTACATCTTCCCTTCCGAATATACAATTAGAACTATTAAGCACGAGTACGTTGAAACTAAGGTGGCTGTGGTTCCTGATAGAAGACCTCTTAGGTTGAGAGCACCTGAACTTCTTAAGGAACAACATGGTTATCTTATTTACGAGATTGAAGGAACAGGTGGTGAATATGTTATTTGTTTGAATAAGGGTTCCACTGTGACACTTTTTACTACTGGTTCTGAAGCTAATAAGACTTCTTGGATCCTTGATGCAAGGCAAATTATTGCTGGTAATGATCCTATTATCGTTTCTCAGGATTCTATTACTATGGGTTCTACTACCGTTCACCTTAGGAATCATGATTTCGATTGCCTTAGAATTGTTACTTCTAGAGAGGAGCTTCTTCAAGTTGATTCTGCTTCAAGACAAACTAGGGTTCTTTCTGAAGATGCTTCAAAGTGGCTTGATACTAAAGAAACTATTCATGAGCATCTTCATAAGCTTGCTGAAGAGCATAGGCTTCTTGGTCAATTCGTTGTTGTGAATAACTATTCTGTTCAAGAACAGTCTGATATTGGAACTCTTTACAGAGGTGTTGGTAGAGCACATGATAGAATGCTTTATACTGATGTTGAGGATGAAACTCTTACTAGGGATGCTGTGCTTGCTGGTGTTACTGGTGAAGATGCTTACTTTTATAATGTTAAGCAGAACGCTTTGTGGAGAGTTAATGCTGGTACTCACCAAGTTATTACTAAGTATTATCCTACAGTGCTTGAAGATCCTGATTCTAAAATGGCTAGAGTTGAGCAAAGAGGTAATATCGTTGTTATGGCTATGTCAAGACTTAATCATTCTGATAAGGGTGTTAATGGTATTATTTACCTTGTTCACCAAGAATCTATGGTTCTTACTGCTGTTATTGGAAATTCAGAAATGCTTAAGAGGCTTAGACAAGCTAGCACAATCTCCCTTACTGAACTTAGGGGAGTGAACGATATGGCTTTGGTTTCTACTATGGATAAGCTTCCTGGTCAGAAGGTTGTTGCTACTAATGCTGAGTTCGTTACAATTCTTGCAAAGGATGAACAGGGATCTCAAGTTTGTTTTTGGCTTAGAACTGTTGATGGTGTTCTTATTAAGCCTTCTATTCAACCACCTATCCCTACAGATCTTACTCTTACTGTTTCTTCTCATAAAGTTGCTGGTCAAGATGTTTTCTATTTTTATTCCATCAAGGAGCAAAGGGTTTATAGACAACAAGGTACCGGACAAACAAATGATACCTCTGTTGGATCTATTCCTATCCCTGGACTTTCTAATCTTTTCACTGTTTATGGTGAGGTTTTTGCTACAACTAATGATGGTAGAATTCTTAGACTTCTTGGTGATGGATCTACTTATCTTGAGGGTATTAACGAACATTGGCTTATTAAGCATCCTAAGTGGTGGCAAGATTTGGCTACTCTTGAAGCAGAGAAGGCTACAACCATTGCTGTTTTCGGTGTTAGGGATCCTCAAAATGCTGTGGTGCCTATTTGTAATGGTGAGGCTTGGCTTTTCGATCTTGATGGTGGTATCCTTTATTCTCAACCTTTGGTTGATTCTCAGGCTGTTGATGCTATTTTCTCTCTTGATGGTAAGCTTATTGCTCCTAATCTTATTCCTCAAGTTCAACAAATGCTTTCTGGTCAGCATCTTAGGAATCCTGTTATTGTTGATGGTAGGCTTCATGTTACTACCATTGATGGTGTTGTTGTTGGTTTCGATAAGAAGTCTCCTACTCTTGTTGCTGGTAATAAGGCTTGGCAAATTTCTAGACAAAATAGGCTTGAGGAAGCTTTGAGAAATTTGTTGTCTAATTGGTCTGATCATAACGATGCTATTGTTCTTCAAGGTGATCCTGAGTCTGCTCCAGCATGGTATTATGTTCCTTCAAGAGAGATTGTGACTGCTAATGGTTTGACTTGGGCTGATCACCCTCTTTGGCTTGGTATGGCTGTTGGTGCTGCAACTCCTTATATTTATTCTACTTCTAGATGCAGAGTGTATGCTGTGGAATCTAAGAATATGGCTAAGGATAAAGGTGAATTTGCTGCTGCTCATTGTTTCGCTAAGTCTTTGGTTCTTCAATCTATTGTTCCTGCTGAAATTACTATTCCTATGCTTGCTGGTATTGATTTCGCTTTGGTTGCTGCTACTAAGGCTACTATTGTTATTGATAGAGATAACTGGAAGCATTATAAGGCTATTGTTTTGGATAACAAGGTGGATCAAGATGGTGGTGCTAAGGTTGTTGTTGGAAGTGAGATTCCTATGAAGGTTTTGGTGAGAAAGTTTCAAGGTAATTTGGAATTTTTCGATCCTAATAACGGTAATGTTGTTATTTTGCAAAACGTTCTTAAGGGTGATGGTGCTTGTTCTCAGATTGAAATTAAAGTTGGTGATGCTGAGACTATGCTTGTGTCTGATTTCTCTGAAATTTTTTCTAAGACCCATCCTCTTGTTGCTTCTCAGGTTTTCGAATTGGGTGATGGTTGTGATTGTAGTCATAAGACTCAAATTGGTTTGTGTGGTTCTGTTGGTCATTCTACTCATGATATTGCTTATATTTCCCATGCTTATTTGCATAGATGGCCTATTTATGTTATCTCTACTTTGATTGCATGA
SEQ ID NO.10：
ATGGAAGAAGCTGATATGGAAAATAGAGGAAATGAAGAAAGACATAGCCAAAGCCAAAGCGCTACTCCTTTGAGAAGAAGAAAGAGAAGCCCTGTTTTCGGTAGAAGCAGCGATGATATTCATAGCTTCAGCAAGAATCCTGAAGAATTCCTTACTAGAAACACACTTTCATGTTCACTTTTGACCAGGACAGCTGAAGCAGTTACAAAGTCTCCAGCTCTTCCTGCACAAGGATGGGTTAAAGTTGGTAGAGTTCCTGGAAGAGGTGATGTTCTTTTCGTTCAACTTGTTGCTGAAAAGTCTGATGAAACTGTTAAAGCTTATTTGCTTGGTTATAACGGTGAGAATGATTTTCCTAAGCCTGCTTATGTTGATATTCCTAAGAGGGGTGCTAAGAATGGTACTTTTCTTTTTACAGTGGATCTTTCTGGTTGTTCTGTTGTTGTTACTCAATTGGATGAGCAAACTTATAGAGTGTTCCATGATAAGAGGGTTAATTCTTCAATTCTTTACGATAACGTGGTTATGTATGTTGATTTTAACCATTACAGGTACCCTAGGGCTCACGGTCATGGTAGAGATACTGGTCAAGCTGTTGTTTGTATGCAATTTGCTGATGGTGTTTGGAAGTTGTTCTTGCAAAGACATGAGACTGCTAATTCTCTTCCTAAGATTTGGTCTAGAGGTGATGGTAAACCTGAAATTTTCTCTGATGCTGATCCTAGACCTCATATGGAGGATGGTATTAAGGAGTTCCTTAATAGAAGGAGGGCTTTGCAAGATGAATTGAAGAAGTTCGCAGATATGTTCGGAGTTGATCCTGAAATTGTTTCTAATGCAGTGGATGGTAAGTATGAAGGTGGACAATTTTCTAGAAATGATGATGCTATTAGGGGTTGGAACGAATTGAGAGCTAGAATGAGACAGAAGATGGCTGAAAGGAAGAAGCTTCTTGAAACTAAGAAGGGAATGTTGGATAGCTTCAATAATCCAGATCATCCTCTTTATGAATTCAGGAAGCAAGTTTTGGATGAATTGAAAGAAATGAGCGCTTATGATAATACTCTTGAAGCTAGCGAGATTGCTGATAGGACTTGGTTGTGGCTTGAAATTAAGAAGGCTAGAGGAACTAACGCAGTTGTTAATAGCGGAAGCGCACCTAAGCAACCTTCCCTTACTGTTGGTGAAAGATATGCAGAGATGGAAGCAGCACTTTGGATGAGAAGCGATACAGCTGCTTTCGAACAAGGTTATGAAAGTTACCAAACTGTTAGAGTTCCTGGTTATAATGAAGATATGACTAGCATTGCTATGAAGAAGCTTTTTGTTGAAAGTAGCCTTACAGATGAACAACTTGGTGCTCTTTGTAAGAGGATTTCTATTGCTAGTGATCAAGAAAATCACCTTTTGAGACAGAAGACTCAAGAAATTGAGAATACCTTCAAGCAATTCTCCGATGGAAGCTTCCTTGAAAGTGCTCCACAAGATTTCTTCCTTAGGAGAGTTGGTGATAAGAGCGGTGGTAGGTGTTATCCTTTGGTTAGAGTTATGGCTGTTGCTCTTTCTCAAGGTGAAGATGTTGCTCAAAATTTCATGGAAAATTTGAGGACTCTTAGTAGGAATCCTGAGTATTCTGGTGAGTCAGCTTCTTCTCAGGCTACCAATGCTGGTCTTTTTAGAGATGCTCTTGCTGGTTTGCATGCTAACGTTGAAGCTATCGCTGCTTCTGAAAAGCTTCCAGCTGTTTTCGGTTTGCCTGATGTTGCTACTATGCTTTCTCAAGCTTCTGAATCTAAGGTTTATGCTCTTAATACTGAAACTCACTCTATGCTTGTTGCTGTTATTGCTGATGAACAATCTCTTGCTCCTAAGTATTATTTCTATGATCCTAATTTCCTTATGGCTGGTTTCTCAGATATTACTAGATTCTCCGAAGCTCTTAAGTATCATTTTGTTAATCTTCAGTTCGCTGGTAATTATAAGGCTTTCGGTACTGCTGAAGCTCCTCAATTCAATCTTGTTCTTATTGATACTACCGCTATGGCTGATGCTCATGTTGATGGTGGTATTACTGTGAAACAACTTTCTGAGGGTGTTGATTTGACTAATGTTTTTGATTCTACCAAGAAGGCTAGGGTTGTTACCGAAGCAGCTGCTAGGTTGGCTGAAGATCAGCAGTTGAGAACTGGACTTACAATTATCGAAGCTGATCAGTGGGCTAACAGATGGCAGGATGCTGTTATCAGACTTAGACTTAAGGAAGGACTTTCTGGTGAGTGGATGCCTTTGCTTGAAAAGATGCAAGAAAATGCAGATGGTAGTTATCAGATTGATTTCATTAACACTAAGAACACTGGTGAAATTAGAGGTGTGTCAACTAGAGATGCTACTTTTAAGGAATTTAAGTCTTACCTTGATAAGAACCTTGATGCTTTGCATAAGGATTTCATTTTCCAGGATGGAGTGTTCAAACCTTTGGAGGGTGAGCCTATTGATGGTTTGAATGCTATGTTCGCTGTTCAGATGATTATTGATTGGTTCAATAGAGGTACTAACGCTGAGGAAGATGCTGCTTCTGGTTCTACTAATGCTCAGTTGTCTCTTGCTATTAAGGTTCATTCTTATATCAACATGGCTCAAGTGGCTCACACTGCTGTTACAGATGTTGCTAAGGTTTATTCTCTTGTTAAGTCTGCTCTTTACGGTGAACAAGTTGCTGGTGGTGCTTCTCTTCTTACTTTCGGTAAGGCTCTTAAATTCGCTGCTACTGAAGGTTTGGGTGTTTTGTTGGGTGCTTCTTTCGTTATTCTTGATGCTTATCAGCTTTCTCTTGTTGAAAATGATGTTCAAAAGGCTATTATCGGTACTCAGTTGGCTTTCGATTCTGCTTCTTTTTTGGTTTCTATTGGTGCTGCTGTTGCTGGTGTTTCTGGTGTTGGTATTGTTCTTTCTGGTGCTGGTGTTCTTTTGGCTGGTTTGGGTGTGGGTTTTGGTGCTTTGGCTCAGGCTTTTGGTCAGATTACTGCTGATGCTCAGTTGGTTGGTAAGTATTTTGCTTTGTTGGATGAGGCTTATTCTGGTGGTGGTTATAGGTATGAGTCTAAGGATAAGGCTTTGATGCCTTTGGCTGGTGCTGTGATTGATAAGATTAGACTTGCTGATAAGCATTTGCAAGTTCATTTTGATTCTCAATTCATCTACAGGACTCATCATGGTTCTACTGGTTCTGGTAAGATTAATTATTTCTTCTGGGCTGGTGATATGCCTCAAATGGTTCATGATAGATCTCAAGCTATTAATGTTAGGGAGCAGATTGGTTATAAGAATGATTCTGCTACTTTGGAATTGCCTGAAGATGCTTCTGATTTGATTTTGCCTGCTACTCCTAAGTCTTTCATTACATATAGATACGAGATCTTGCCATTTGCTACTTCTAGGCATGATAGAGGTTTTGATGTTATTAGAAGATTGGAAGAAGATTACAGGTTCGATTATGATTTCTACATTTTCCCTTCCGAATATACTATTAGGACTATTAAGCATGAGTACGTTGAAACTAAGGTGGCTGTTGTGTTGGATAGAAGATCTTTGAGATTGGGTGCTCCTGAGCTTTTGAAGGAGCAGCATGGTTATCTTATTTATGAAATTGAGGGTACTGGTGGTCAATATGTGGTTTGTTTGAATCATGGTAGTACTGTGACTTTGAAGACTACTGGTTCAGAAGCAAATAAGACACTTTGGATTTTGGATGCTAGACAGTTGATTGCTGGTAATGATAGTATTACTGTTTCTCAAGATTCCATTACTATCGGATCTACTACTGTTCACCTTAGAAATCAAGATTTCGATTGCCTTCAAATTGTTATGAGTAGAGAAGAGCTTTTGCAGGTGGATCTTGCTTCAAGACAGGCTAGGGTTTTGTCAGAAGATGCTTCAAAATGGCTTGATACAAAGGAAACTATCCATGAACACCTTCACAAGTTGGCTGAAGAACATAGACTTCTTGGTCAATTCGTGGTTGTGAATAATTATAGTGTTCAAGAACAGAGTGATATCGGTACTCTTTACAGAGGAGTTGGTAGAGCTTATTACGATGCTGCTCATGATAGAATGCTTTATACTGATGTTGAGGATGAGACTTTGACTAGGGATGCTGTTTTGGCAGGTGTGACTGGTGAGGATGCATATTTTTATAATGTGAAACAGAACGCTCTTTGGAGAGTTAATGCTGGTACTCACCAAGTTATTACTAAGTATTATCCTACAGTGTTGGAAGATCCTGATTCTAAGATGGCTAGAGTTGAACAGCATGGTAATATTGTTGTTATGGCTATGTCTAGGTTGAATCAACCTGATAAGGGTGTTGATGGAATTATTTATTTGGTGCATCAAGAATCTATGGTGTTGACTGCTGTTATTGGTAATTCTGAGATGCTTAAGAGACTTAGACAAACTTCAACTATTTCTCTTACTGAATTGAGGGGTGTGAACGATATGGCTCTTGTTTCTACTATGGATAAGCTTCCAGGTCAGAAGGTGGTTGCTACTAACGCAGAATTCGTTACAATTTTGGCTAAGGATGAACAAGGAAGCCAGGTTTGTTTTTGGCTTAGGACTGTTGATGGTGTTTTGATTAAGCCTTCCATTCAACCTCCTATTCCTACAGATCTTGCTCTTACTGTTTCTTCTCATAAAGTTGCTGGTCAAGATGTTTTTTATTTCTGTTCTATCAAGGAGCAGACTGTTTATAGACAACAAGGTACCGGACAAACCAACGATACATCTGTTGGTTCTATTCCTATCCAAGGTCTTTCTAATCTTTTCACTGTTTATGGTGAGGTTTTCGCTACTACTGATGATGGTAGGATTCTTAGATTGCTTGGTGATGGTTCTACTTATCTTGAGGGTATTAACGAACATTGGTTGATTAAGCATCCTAAGTGGTGGCAAGATTTGGCTACTCTTGAAGCAGAGACTGCTACTACTATTGCAGTTTTCGGTGTTAGGGATCCTCAAAATGCTGTTGTTCCTATTTGGTATCATTCCGGTAAGATTGCTGTTGCTTCTTCTAAGCTTTATGGTAAGCAGCTTCAATTTCTTGGTTTCCATTCTAATGATCAAGCTTGGCTTTTCGATCTTGATGGTGGTATCTTGTATTCTCAACCTCTTGTTGATAGGCAAACTGTTGATACTATTTTCTCCCTTGATGGTAAGCTTATTGCTCCTGATTTGATTCCTCAAGTTCAACAAATGCTTTCTGGTCAGCACCTTAGAAACCCTGTTATTGTTGATGGTAGACTTCATGTGACTACCATTGATGGTGTTGTTGTGGGTTTCGATAAGAAGAGCCCTACTCTTGTTGCTGTGAATAAGGCTTGGCAAATTTCCAGGCAAAATAGGTTGGAGGAGGCTCTTGGTAATTTGCTTTCTAATTGGTCTGATCATAACGATGCTATTGTTCTTCAAGGTGATCCTGAGTCTGCTCCTGCATGGTATTACGTTCCTTCTAGAGAAATTGTGACTGCTAATGGTCTTACTTGGGCTGATCATCCTTTGTGGCTTGGTATGGCTGTTGGTGCTGCTACTCCTTATATTTATAATACTTCTCCTAGGAGGAGGTCTATTGTTCCTGAAGAGATTACTATCCCTACTCTTGCTGGTATTGATTTCGCTTTGGTTGCTGCTACTAATGCTACTATTGTTATTGATAGAGATAACTGGAAGCATTATAAGGCAATTGTTCTTGATAACAAGGTTGATCAAGATGGAGGTGCTAAGGTTGTGTTGGGTTCTGAGATTCCTATGAAGGTTTTGGTTAGAAAGTTTCAAGGTAATTTGGAATTCTTCGATCCTAATAATGGTAATGTTGTGATTTTGCAAAACGTTCTTAAGGGTGATGGTGCTTGTTGTCAGATTGATATTAAAGTTGGTGATGCTGAAACTATGCTTGCTTCTGATTTCTCTGAGATTTTCTCCAAGACCCATCCTCTTGTTGCTTCTCAGGTTTTCGAATTGGGTGATGTTATTGCTGTTACTAAGCATAAGTCTGATTATGAGGATCTTTGGGCTATTTTGCATATGACTTGA
本发明提供编码所述的抗虫性的苏铁毒蛋白基因及其同源基因的蛋白质，所述蛋白质的氨基酸序列如下：
SEQ ID NO.11
Met Val Met Val Ile Asp Tyr Pro Ser Leu Arg Ala Ser Leu Tyr Ala ThrLys Ser Ala Val Pro Cys Arg Pro Leu Pro Gly Asn Pro Glu Arg Val Ser Arg ArgVal Ser Arg Arg Gly Ala Gly Glu Ile Gly Ser His Ala Asp Phe Trp Ile Ser ArgAla Lys Ala Ser Ala Ile Arg Ile Phe Pro Arg Gln Glu Gly Pro Leu Arg Glu SerArg Arg Arg Phe Tyr Leu Leu Arg Glu Ala Val Phe Thr Gly Arg Arg Thr Ala TrpAla Ala Asn Pro Ala Ala Gln Arg Ala Ala Trp Asp Arg Lys Arg Ser Pro Phe PheGly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu ThrGln Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr Ala Gln Thr Val Thr Lys SerPro Val Leu Pro Ala Gln Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly AspAla Leu Phe Val Glu Leu Val Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr LeuLeu Gly Tyr Asn Gly Lys Asn Asp Ser Pro Glu Pro Ala Tyr Val Asp Ile Pro LysArg Gly Ala Glu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys Ser ValVal Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp Lys Arg Val AsnSer Ser Ile Leu Tyr Asp Asn Val Val Met Tyr Val Asp Phe Asn His Tyr Arg TyrPro Arg Ala His Gly His Gly Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln PheAla Asp Gly Val Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu ProLys Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro ArgPro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala Leu Gln AspGlu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp Pro Asp Ile Val Ser Asn AlaVal Asp Gly Glu Tyr Glu Gly Gly Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg GlyTrp Asn Glu Leu Arg Ala Ser Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu LeuGln Thr Lys Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr LysPhe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp Asn Thr LeuGlu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu Ile Lys Lys Ala LysGly Thr Asn Ala Val Val Asn Ser Gly Ala Ala Pro Lys Gln Pro Ser Leu Thr ValGly Glu Arg Tyr Ala Glu Met Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala AlaPhe Glu Gln Gly Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu AspMet Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp Glu GlnLeu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln Glu Asn His Leu LeuThr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe Lys Gln Phe Ser Asp Gly Ser PheLeu Glu Ser Ala Pro Gln Asp Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly GlyArg Cys Tyr Pro Leu Val Arg Val Met Ala Val Ala Leu Ser Glu Gly Glu Asp ValAla Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser GlyGlu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala GlyLeu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe GlyLeu Pro Asp Val Ala Thr Met Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala LeuAsn Thr Glu Thr His Ser Met Leu Val Ala Val Ile Val Asp Lys Gln Ser Leu AlaArg Lys Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile ThrArg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn TyrLys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr ThrAla Met Glu Asp Ala His Val Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu GlyVal Asp Leu Thr Asn Val Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu AlaAla Ala Arg Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu AlaAsp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu GlyLeu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu Asn Ala Asp Gly SerTyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr Gly Glu Ile Arg Gly Val Ser ThrArg Asp Ala Thr Phe Lys Glu Phe Lys Ser Tyr Leu Asp Lys Asn Leu Asp Val LeuHis Lys Asp Phe Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro IleAsp Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg GlyThr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu AlaIle Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala His Thr Ala Val Thr AspVal Ala Lys Val Tyr Asn Leu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala GlyGlu Thr Ser Leu Phe Thr Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly LeuGly Val Leu Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu ValGlu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala SerPhe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly Val Gly Met Ile LeuGly Gly Ala Gly Val Val Leu Gly Gly Leu Gly Val Gly Phe Gly Ala Leu Ala GlnAla Phe Gly Glu Ile Thr Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu LeuAsp Glu Ala Tyr Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu MetPro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln ValHis Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly LysIle Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro Gln Met Val His Asp Arg Ser GlnAla Ile Asn Val Arg Glu Gln Ile Gly Tyr Lys Asn Asp Asn Ala Thr Leu Glu LeuPro Glu Asp Ala Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr TyrArg Tyr Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Thr Gly Phe Asp Val IleArg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser GluTyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr Lys Val Ala Val Val LeuAsp Arg Arg Ser Leu Arg Leu Gly Ala Pro Glu Leu Leu Lys Glu Gln His Gly TyrLeu Ile Tyr Glu Ile Glu Gly Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn Gln GlySer Thr Val Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile LeuAsp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser IleThr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe Asp Cys Leu Gln IleVal Met Ser Arg Glu Glu Leu Leu Gln Val Asp Leu Ala Ser Gly Glu Ala Arg ValPhe Ser Glu Asp Ala Ser Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His LeuHis Lys Leu Ala Glu Glu His Arg Leu His Gly Gln Phe Val Val Val Asn Asn TyrTyr Arg Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr ThrAsp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly GluAsp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val Asn Ala Gly ThrHis Gln Val Ile Thr Lys Tyr Tyr Pro Thr Leu Leu Glu Asp Pro Asp Ser Asn MetAla Arg Val Glu Gln His Gly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn HisPro Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Ile Val LeuThr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr IleSer Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr Met Asp ArgLeu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val Thr Leu Leu Ala LysAsp Glu Gln Gly Ala Asn Ile Lys Glu Gln Thr Val Tyr Cys Gln Gln Gly Thr GlyGln Thr Asn Asp Thr Ser Ile Glu Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu LeuThr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly Arg Ile Leu Arg Leu LeuGly Asp Gly Ser Thr Cys Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His ProLys Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr Thr Val Ala ValPhe Gly Val Arg Asp Leu Gln Asn Ala Val Val Pro Ile Trp Tyr His Asn Gly LysIle Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe AsnSer Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln ProLeu Val Asp Ser Phe Leu Tyr Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala ProIle Phe Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg Asn Pro ValIle Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val Val Gly Phe AspLys Lys Ser Pro Thr Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg Gln AsnArg Leu Glu Glu Ala Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp AlaIle Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser ArgGlu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly MetAla Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg Arg Arg Val Tyr ValVal Glu Ser Lys Asn Met Ala Lys Asp Lys Gly Glu Phe Ala Ala Ala His Cys PheAla Lys Ser Leu Val Leu Gln Ser Ile Val Pro Glu Glu Ile Thr Ile Pro Thr LeuAla Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile Asp ArgAsp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp GlyGly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu Val Arg Lys PheGln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn Val Val Ile Leu Gln AsnVal Leu Lys Gly Asp Gly Ala Cys Cys Gln Ile Glu Ile Lys Val Gly Asp Ala GluThr Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val AlaSer Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser Asp TyrAla Asp Leu Trp Ala Ile Leu Arg Met Thr*
SEQ ID NO.12：
Met Glu Glu Ala Asp Met Glu Asn Arg Gly Tyr Glu Glu Arg His Ser GlnSer Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Phe Phe Gly Arg SerSer Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu Thr Lys Asn ThrLeu Ser Cys Ser Leu Leu Thr Arg Thr Ala Gln Thr Val Thr Lys Ser Pro Val LeuPro Ala Gln Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu SerGly Cys Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His AspLys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met Tyr Val Asp Phe AsnHis Tyr Arg Tyr Pro Arg Ala His Gly His Gly Arg Asp Thr Gly Gln Ala Val ValCys Met Gln Phe Ala Asp Gly Val Trp Lys Leu Phe Leu Gln Arg His Glu Thr AlaAsn Ser Leu Pro Lys Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser AspAla Glu Pro Arg Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg ArgAla Leu Gln Asp Glu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp Pro Glu IleVal Ser Asn Ala Val Asp Gly Glu Tyr Glu Gly Gly Gln Phe Ser Arg Asn Asp AspAla Ile Arg Gly Trp Asn Glu Leu Arg Ala Ser Met Arg Gln Lys Met Ala Glu ArgLys Lys Arg Leu Glu Arg Asn Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp HisPro Leu Tyr Lys Phe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala TyrAsp Asn Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu IleLys Lys Ala Lys Gly Thr Asn Ala Val Val Asn Ser Gly Ala Ala Pro Lys Gln ProSer Leu Thr Val Gly Glu Arg Tyr Ala Glu Met Glu Ala Ala Leu Trp Met Arg SerAsp Thr Ala Ala Phe Glu Gln Gly Tyr Glu Ser Tyr Gln Thr Leu Arg Val Pro GlyTyr Asn Glu Asp Met Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser LeuThr Asp Glu Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln GluAsn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe Lys Gln Phe SerAsp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp Phe Phe Leu Arg Arg Val Gly AspLys Ser Gly Gly Arg Cys Tyr Pro Leu Val Arg Val Met Ala Val Ala Leu Ser GlnGly Glu Asp Val Ala Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn ProGlu Tyr Ser Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg AspAla Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu ProAla Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser Gln Ala Ser Glu Ser LysVal Tyr Ala Leu Asn Thr Glu Thr His Ser Met Leu Val Ala Val Ile Val Asp LysGln Ser Leu Ala Arg Lys Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Ile Ala Gly PheSer Asp Ile Thr Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln PheAla Gly Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val LeuIle Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly Gly Ile Thr Val Lys GlnLeu Ser Glu Gly Val Asp Leu Thr Asn Val Phe Asp Ser Thr Lys Lys Ala Arg ValVal Thr Glu Thr Ala Ala Arg Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu ThrIle Ile Glu Ala Asp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu LysLeu Lys Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu AsnAla Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr Gly Glu Ile ArgGly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe Lys Ser Tyr Leu Asp Lys AsnLeu Asp Val Leu His Lys Asp Phe Ile Phe Gln Asp Gly Val Phe Lys Pro Leu GluGly Glu Pro Ile Asp Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp TrpPhe Asn Arg Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala GlnLeu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala His ThrAla Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys Ser Ala Leu Tyr Gly GluGln Val Ala Gly Glu Ala Ser Leu Leu Thr Phe Gly Lys Ala Leu Lys Phe Ala AlaThr Glu Gly Leu Gly Val Leu Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr GlnLeu Ser Leu Val Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala PheAsp Ser Ala Ser Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly ValGly Met Ile Leu Gly Gly Ala Gly Val Val Leu Gly Gly Leu Gly Val Gly Phe GlyAla Leu Ala Gln Ala Phe Gly Glu Ile Thr Ala Asp Ala Gln Leu Val Gly Lys TyrPhe Ala Leu Leu Asp Glu Ala Tyr Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys AspLys Ala Leu Met Pro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp LysHis Leu Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Leu ThrGly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro Gln Met Val HisAsp Arg Ser Gln Ala Ile Asn Ile Arg Glu Gln Ile Gly Tyr Lys Asn Asp His AlaThr Leu Glu Leu Pro Glu Asp Ala Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys SerPhe Ile Thr Tyr Gln Tyr Gln Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg GlyPhe Asp Val Ile Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr IlePhe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr Lys ValAla Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala Pro Glu Leu Leu Lys GluGln His Gly Tyr Leu Ile Tyr Glu Ile Glu Gly Thr Gly Gly Gln Tyr Val Ile CysLeu Asn Gln Gly Ser Thr Val Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys ThrLeu Trp Ile Leu Asp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val SerGln Asp Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe AspCys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln Val Asp Leu Ala Ser GlyGlu Ala Arg Val Phe Ser Glu Asp Ala Ser Lys Trp Leu Asp Thr Lys Glu Thr IleHis Glu His Leu His Lys Leu Ala Glu Glu His Arg Leu His Gly Gln Phe Val ValVal Asn Asn Tyr Tyr Arg Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp ArgMet Leu Tyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala GlyVal Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg ValAsn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Leu Leu Glu Asp ProAsp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn Ile Val Val Met Ala Met SerArg Leu Asn His Pro Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln GluSer Met Val Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg GlnThr Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val SerThr Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val ThrIle Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp Leu Arg Thr Val AspGly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr Asp Leu Thr Leu ThrVal Ser Ser His Lys Val Ala Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys GluGln Thr Val Tyr Cys Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Ile Glu SerIle Pro Ile Pro Gly Leu Ser Asn Leu Leu Thr Val Tyr Gly Glu Val Phe Ala ThrThr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr Tyr Leu Asp GlyIle Asn Glu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala Thr LeuGlu Ala Glu Lys Ala Thr Thr Val Ala Val Phe Gly Val Arg Asp Leu Gln Asn AlaVal Val Pro Ile Trp Tyr His Asn Gly Lys Ile Ala Val Ala Ser Ser Lys Leu TyrGly Lys Gln Leu Gln Phe Leu Gly Phe Asn Ser Asn Gly Glu Ala Trp Leu Phe AspLeu Asp Asp Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Ser Phe Leu Tyr Ala IlePhe Ser Leu Asp Gly Lys Leu Ile Ala Pro Ile Phe Ile Pro Gln Val Glu Gln MetLeu Ser Gly Gln His Leu Arg Asn Pro Val Ile Val Asp Gly Arg Leu His Val ThrThr Ile Asp Gly Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala ValAsn Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly Asn LeuLeu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln Gly Asp Pro Glu SerAla Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile Val Thr Ala Asn Gly Leu ThrTrp Ala Asp His Pro Leu Trp Leu Gly Met Ala Val Gly Ala Ala Thr Pro Tyr IleTyr Ser Thr Ser Arg Arg Arg Val Tyr Ala Val Glu Ser Lys His Met Ala Lys AspLys Gly Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser IleVal Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Ile Asp Phe Ala Leu Val AlaAla Thr Asn Ala Thr Ile Val Ile His Arg Asp Asn Trp Lys His Tyr Lys Ala IleVal Leu Asp Asn Lys Val Asp Gln Asp Gly Gly Ala Lys Val Val Val Gly Ser GlyIle Pro Met Lys Val Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp ProAsn Asn Gly Asn Val Val Ile Leu Arg Asn Val Leu Lys Gly Asp Gly Ala Cys SerGln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp Phe Ser GluIle Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val Phe Glu Leu Gly Asp ValIle Ala Val Thr Lys His Lys Ser Asp Tyr Ala Asp Leu Trp Ala Ile Leu Arg MetThr
SEQ ID NO.13：
Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn Phe Met GluAsn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly Glu Ser Ala Ser Ser GlnAla Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala Gly Leu His Ala Asn Val GluAla Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala ThrMet Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His SerMet Leu Ala Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr Tyr Phe TyrAsp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe Ser Glu Ala LeuLys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr Lys Ala Phe Gly Thr AlaGlu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr Thr Ala Met Ala Asp Ala HisVal Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn ValPhe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg Leu Ala GluAsp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln Trp Ala Asn ArgTrp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu Gly Leu Ser Gly Glu Trp MetPro Leu Leu Glu Arg Met Gln Glu Asn Gly His Gly Ser Tyr Gln Ile Glu Phe LeuAsn Thr Lys Asn Thr Gly Glu Thr Arg Trp Val Ser Thr Arg Asp Ala Thr Phe LysGlu Phe Lys Ser Tyr Leu Asp Lys Asn Phe Asp Ala Leu Arg Lys Asp Phe Ile PheGln Asp Gly Val Phe Lys Pro Leu Asp Gly Glu Pro Ile Asp Gly Leu Asn Ala MetPhe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Pro Gly Thr Asn Ala Glu Glu AspAla Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu Ala Ile Lys Val His Ser TyrIle Asn Met Ala Gln Val Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr AsnLeu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Glu Ala Ser Leu Leu ThrPhe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu Leu Gly AlaSer Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn Asp Val Gln LysAla Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala Ser Phe Leu Ala Gly Ile GlyAla Ile Val Ala Gly Ala Ser Gly Val Gly Met Ile Leu Gly Gly Ala Gly Val IleLeu Gly Gly Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Gln Ile ThrAla Asp Ala Gln Leu Val Gly Glu Tyr Phe Ala Leu Leu Asp Glu Ala Tyr Ser GlyGly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu Ala Gly Ala ValIle Asp Lys Ile Arg Leu Ala Asp Lys Arg Leu Gln Val His Phe Asp Ser Gln PheIle Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe TrpVal Gly Asp Met Pro Gln Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg GluGln Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser AspLeu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr Glu Ile Leu ProPhe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg Leu Glu Glu AspTyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser Glu Tyr Thr Ile Arg Thr IleLys His Glu Tyr Val Glu Thr Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu ArgLeu Gly Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile GluGly Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn His Gly Ser Thr Val Thr Leu LysThr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp Ala Arg Gln Leu IleAla Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser Ile Thr Ile Gly Ser Thr ThrVal His Leu Arg Asn Gln Asp Phe Asp Cys Leu Gln Ile Val Met Ser Arg Asp GluLeu Leu Gln Val Asp Leu Ala Ser Arg Glu Val Arg Val Phe Ser Glu Asp Ala SerLys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala Gly GluHis Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr Arg Gly Val Gly ArgAla Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr Thr Asp Val Glu Asp Glu ThrLeu Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly Glu Asp Ala Tyr Phe Tyr AsnVal Asn Gln Asn Ala Leu Trp Arg Val Asn Ala Asp Thr His Gln Val Ile Thr LysTyr Tyr Pro Thr Leu Leu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln HisGly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp Lys Gly Val AsnGly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu Thr Ala Val Ile Gly AsnSer Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr Ile Ser Leu Thr Glu Leu ArgGly Val Asn Asp Met Ala Leu Val Ser Thr Met Asp Arg Leu Pro Gly Gln Lys ValVal Ala Thr Asn Ala Glu Phe Val Thr Leu Leu Ala Lys Asp Glu Gln Gly Ser GlnVal Cys Phe Trp Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln ProPro Ile Pro Thr Asp Leu Thr Leu Thr Val Ser Ser His Lys Val Ala Gly Gln AspVal Phe Tyr Phe Tyr Ser Ile Lys Glu Gln Thr Val Tyr Cys Gln Gln Gly Thr GlyGln Thr Asn Asp Thr Ser Ile Glu Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu LeuThr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly Arg Ile Leu Arg Leu LeuGly Asp Gly Ser Thr Tyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His ProLys Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr Thr Val Ala ValPhe Gly Val Arg Asp Pro Gln Asn Ala Val Val Pro Ile Trp Tyr His Asn Gly LysIle Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe AsnSer Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln ProLeu Val Asp Ser Gln Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile AlaPro Asn Leu Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg Asn ProVal Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val Val Gly PheAsp Lys Lys Ser Pro Thr Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg GlnAsn Arg Leu Glu Glu Ala Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn AspAla Ile Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro SerArg Glu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu GlyMet Ala Val Gly Ala Ala Thr Leu Tyr Ile Tyr Ser Thr Ser Arg Arg Arg Val TyrAla Val Glu Ser Lys Lys Met Ala Lys Asp Lys Gly Glu Phe Ala Ala Ala His CysPhe Ala Lys Ser Leu Val Leu Gln Ser Ile Val Pro Glu Glu Ile Thr Ile Pro ThrLeu Ala Gly Thr Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile AspArg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln AspGly Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu Val Arg LysPhe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn Val Val Ile Leu GlnAsn Val Leu Lys Gly Asp Gly Ala Cys Ser Lys Ile Glu Ile Lys Val Gly Asp AlaGlu Thr Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu ValAla Ser Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser AspTyr Ala Asp Leu Trp Ala Ile Leu Arg Met Thr
SEQ ID NO.14：
Met Glu Glu Val Asp Met Gln Asn Arg Gly Tyr Glu Glu Trp His Ser GlnSer Gln Ser Ala Thr Thr Leu Arg Arg Arg Lys Arg Ser Pro Val Phe Gly Arg SerSer Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu Thr Gln Asn ThrLeu Ser Cys Ser Leu Leu Thr Arg Thr Ala Glu Thr Val Thr Lys Ser Pro Val LeuPro Glu Arg Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu PheVal Gln Val Val Ala Glu Gln Ser Tyr Asp Thr Val Lys Ala Tyr Leu Leu Gly TyrAsn Gly Glu Asn Asp Ser Pro Asn Pro Ala Tyr Val Asp Ile Pro Lys Arg Ala AlaGlu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys Ser Val Val Val ThrGln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp Lys Arg Val Asn Ser Ser IleLeu Tyr Asp Asn Val Val Met Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg AlaHis Gly His Gly Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp GlyVal Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile TrpSer Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro Arg Gln His MetGlu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala Leu Gln Asp Glu Leu LysLys Phe Ala Asp Met Phe Gly Val Asp Pro Glu Ile Val Ser Asn Ala Val Asp GlyLys Tyr Lys Gly Gly Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn GluLeu Arg Ala Ser Met Arg Gln Lys Met Ala Glu Arg Asn Lys Val Leu Leu Thr MetLys Leu Glu Gln Pro Arg Ser Ser Arg Val Leu Pro Leu Val Asn Ala Thr Gln ArgTrp Arg Arg Pro Phe Gly Cys Val Ala Thr Gln Arg Arg Leu Ser Lys Ala Gly ArgLeu Val Gln Ala Tyr Ile His Arg Asp Gln Glu Asn His Leu Leu Thr Gln Lys ThrGln Glu Ile Glu Ser Thr Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser AlaPro Gln Asp Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr ProLeu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn PheMet Glu Asn Leu Arg Thr Leu Ser Lys Asn Pro Glu Tyr Ser Gly Glu Ser Ala SerSer Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala Gly Leu His Ala AsnVal Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp ValAla Thr Met Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu ThrHis Ser Met Leu Ala Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr TyrPhe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe Ser GluAla Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr Lys Pro Phe GlyThr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr Thr Val Met Ala AspAla His Val Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu ThrAsn Val Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg LeuAla Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln Trp AlaAsn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu Gly Leu Ser Gly GluTrp Met Pro Leu Leu Glu Lys Met Gln Glu Asn Ala Asp Gly Ser Tyr Gln Ile AspPhe Ile Asn Thr Lys Asn Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Gly ThrPhe Lys Glu Leu Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys Asp PheIle Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly Leu AsnAla Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg Gly Thr Asn Ala GluGlu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu Ala Ile Lys Val HisSer Tyr Ile Asn Met Ala Gln Val Ala His Thr Ala Val Thr Asp Val Ala Lys ValTyr Ser Leu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala Ser LeuLeu Thr Phe Gly Lys Ala Leu Lys Leu Ala Ala Thr Glu Gly Leu Gly Val Leu LeuGly Ala Pro Val Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn Asp ValGln Lys Ala Ile Ile Gly Thr Gln Leu Asp Phe Asp Ser Ala Ser Phe Leu Val SerIle Gly Ala Ala Val Ala Gly Val Ser Gly Val Gly Ile Val Leu Ser Gly Ala GlyVal Leu Leu Ala Gly Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly GluIle Thr Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala TyrArg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu Ala GlyAla Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln Val His Phe Asp SerGln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly Lys Ile Asn Tyr PhePhe Trp Val Gly Asp Met Pro Lys Met Val His Asp Arg Ser Gln Ala Ile Asn ValArg Glu Gln Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp AlaSer Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr Glu IleLeu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg Leu GluGlu Asp His Arg Phe Asp Tyr Val Phe Tyr Ile Phe Pro Ser Glu Tyr Thr Ile ArgThr Ile Lys His Glu Tyr Val Glu Thr Lys Val Ala Val Val Pro Asp Arg Arg ProLeu Arg Leu Arg Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr GluIle Glu Gly Thr Gly Gly Glu Tyr Val Ile Cys Leu Asn Lys Gly Ser Thr Val ThrLeu Phe Thr Thr Gly Ser Glu Ala Asn Lys Thr Ser Trp Ile Leu Asp Ala Arg GlnIle Ile Ala Gly Asn Asp Pro Ile Ile Val Ser Gln Asp Ser Ile Thr Met Gly SerThr Thr Val His Leu Arg Asn His Asp Phe Asp Cys Leu Arg Ile Val Thr Ser ArgGlu Glu Leu Leu Gln Val Asp Ser Ala Ser Arg Gln Thr Arg Val Leu Ser Glu AspAla Ser Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu AlaGlu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr Ser Val Gln GluGln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly Arg Ala His Asp Arg Met LeuTyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val ThrGly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val Asn AlaGly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Val Leu Glu Asp Pro Asp SerLys Met Ala Arg Val Glu Gln Arg Gly Asn Ile Val Val Met Ala Met Ser Arg LeuAsn His Ser Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser MetVal Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Ala SerThr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr MetAsp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val Thr Ile LeuAla Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp Leu Arg Thr Val Asp Gly ValLeu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr Asp Leu Thr Leu Thr Val SerSer His Lys Val Ala Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys Glu Gln ArgVal Tyr Arg Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Val Gly Ser Ile ProIle Pro Gly Leu Ser Asn Leu Phe Thr Val Tyr Gly Glu Val Phe Ala Thr Thr AsnAsp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr Tyr Leu Glu Gly Ile AsnGlu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala Thr Leu Glu AlaGlu Lys Ala Thr Thr Ile Ala Val Phe Gly Val Arg Asp Pro Gln Asn Ala Val ValPro Ile Cys Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Gly Gly Ile Leu Tyr SerGln Pro Leu Val Asp Ser Gln Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys LeuIle Ala Pro Asn Leu Ile Pro Gln Val Gln Gln Met Leu Ser Gly Gln His Leu ArgAsn Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val ValGly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Gly Asn Lys Ala Trp Gln Ile SerArg Gln Asn Arg Leu Glu Glu Ala Leu Arg Asn Leu Leu Ser Asn Trp Ser Asp HisAsn Asp Ala Ile Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr ValPro Ser Arg Glu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu TrpLeu Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg Cys ArgVal Tyr Ala Val Glu Ser Lys Asn Met Ala Lys Asp Lys Gly Glu Phe Ala Ala AlaHis Cys Phe Ala Lys Ser Leu Val Leu Gln Ser Ile Val Pro Ala Glu Ile Thr IlePro Met Leu Ala Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Lys Ala Thr Ile ValIle Asp Arg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val AspGln Asp Gly Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu ValArg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn Val Val IleLeu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Ser Gln Ile Glu Ile Lys Val GlyAsp Ala Glu Thr Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His ProLeu Val Ala Ser Gln Val Phe Glu Leu Gly Asp Gly Cys Asp Cys Ser His Lys ThrGln Ile Gly Leu Cys Gly Ser Val Gly His Ser Thr His Asp Ile Ala Tyr Ile SerHis Ala Tyr Leu His Arg Trp Pro Ile Tyr Val Ile Ser Thr Leu Ile Ala
SEQ ID NO.15：
Met Glu Glu Ala Asp Met Glu Asn Arg Gly Asn Glu Glu Arg His Ser GlnSer Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Val Phe Gly Arg SerSer Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu Phe Leu Thr Arg Asn ThrLeu Ser Cys Ser Leu Leu Thr Arg Thr Ala Glu Ala Val Thr Lys Ser Pro Ala LeuPro Ala Gln Gly Trp Val Lys Val Gly Arg Val Pro Gly Arg Gly Asp Val Leu PheVal Gln Leu Val Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr Leu Leu Gly TyrAsn Gly Glu Asn Asp Phe Pro Lys Pro Ala Tyr Val Asp Ile Pro Lys Arg Gly AlaLys Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys Ser Val Val Val ThrGln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp Lys Arg Val Asn Ser Ser IleLeu Tyr Asp Asn Val Val Met Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg AlaHis Gly His Gly Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp GlyVal Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile TrpSer Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Asp Pro Arg Pro His MetGlu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala Leu Gln Asp Glu Leu LysLys Phe Ala Asp Met Phe Gly Val Asp Pro Glu Ile Val Ser Asn Ala Val Asp GlyLys Tyr Glu Gly Gly Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn GluLeu Arg Ala Arg Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu Leu Glu Thr LysLys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr Glu Phe Arg LysGln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp Asn Thr Leu Glu Ala SerGlu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu Ile Lys Lys Ala Arg Gly Thr AsnAla Val Val Asn Ser Gly Ser Ala Pro Lys Gln Pro Ser Leu Thr Val Gly Glu ArgTyr Ala Glu Met Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu GlnGly Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu Asp Met Thr SerIle Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp Glu Gln Leu Gly AlaLeu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln Glu Asn His Leu Leu Arg Gln LysThr Gln Glu Ile Glu Asn Thr Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu SerAla Pro Gln Asp Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys TyrPro Leu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln AsnPhe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly Glu Ser AlaSer Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala Gly Leu His AlaAsn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro Ala Val Phe Gly Leu Pro AspVal Ala Thr Met Leu Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr GluThr His Ser Met Leu Val Ala Val Ile Ala Asp Glu Gln Ser Leu Ala Pro Lys TyrTyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe SerGlu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr Lys Ala PheGly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile Asp Thr Thr Ala Met AlaAsp Ala His Val Asp Gly Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp LeuThr Asn Val Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala ArgLeu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln TrpAla Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Arg Leu Lys Glu Gly Leu Ser GlyGlu Trp Met Pro Leu Leu Glu Lys Met Gln Glu Asn Ala Asp Gly Ser Tyr Gln IleAsp Phe Ile Asn Thr Lys Asn Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp AlaThr Phe Lys Glu Phe Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys AspPhe Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly LeuAsn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg Gly Thr Asn AlaGlu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu Ala Ile Lys ValHis Ser Tyr Ile Asn Met Ala Gln Val Ala His Thr Ala Val Thr Asp Val Ala LysVal Tyr Ser Leu Val Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala SerLeu Leu Thr Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val LeuLeu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn AspVal Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala Ser Phe Leu ValSer Ile Gly Ala Ala Val Ala Gly Val Ser Gly Val Gly Ile Val Leu Ser Gly AlaGly Val Leu Leu Ala Gly Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe GlyGln Ile Thr Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu AlaTyr Ser Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu AlaGly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln Val His Phe AspSer Gln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser Gly Lys Ile Asn TyrPhe Phe Trp Ala Gly Asp Met Pro Gln Met Val His Asp Arg Ser Gln Ala Ile AsnVal Arg Glu Gln Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu AspAla Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr GluIle Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg LeuGlu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser Glu Tyr Thr IleArg Thr Ile Lys His Glu Tyr Val Glu Thr Lys Val Ala Val Val Leu Asp Arg ArgSer Leu Arg Leu Gly Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile TyrGlu Ile Glu Gly Thr Gly Gly Gln Tyr Val Val Cys Leu Asn His Gly Ser Thr ValThr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp Ala ArgGln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser Ile Thr Ile GlySer Thr Thr Val His Leu Arg Asn Gln Asp Phe Asp Cys Leu Gln Ile Val Met SerArg Glu Glu Leu Leu Gln Val Asp Leu Ala Ser Arg Gln Ala Arg Val Leu Ser GluAsp Ala Ser Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys LeuAla Glu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr Ser Val GlnGlu Gln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly Arg Ala Tyr Tyr Asp AlaAla His Asp Arg Met Leu Tyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp AlaVal Leu Ala Gly Val Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn AlaLeu Trp Arg Val Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr ValLeu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn Ile Val ValMet Ala Met Ser Arg Leu Asn Gln Pro Asp Lys Gly Val Asp Gly Ile Ile Tyr LeuVal His Gln Glu Ser Met Val Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu LysArg Leu Arg Gln Thr Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp MetAla Leu Val Ser Thr Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn AlaGlu Phe Val Thr Ile Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp LeuArg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr AspLeu Ala Leu Thr Val Ser Ser His Lys Val Ala Gly Gln Asp Val Phe Tyr Phe CysSer Ile Lys Glu Gln Thr Val Tyr Arg Gln Gln Gly Thr Gly Gln Thr Asn Asp ThrSer Val Gly Ser Ile Pro Ile Gln Gly Leu Ser Asn Leu Phe Thr Val Tyr Gly GluVal Phe Ala Thr Thr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser ThrTyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln AspLeu Ala Thr Leu Glu Ala Glu Thr Ala Thr Thr Ile Ala Val Phe Gly Val Arg AspPro Gln Asn Ala Val Val Pro Ile Trp Tyr His Ser Gly Lys Ile Ala Val Ala SerSer Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe His Ser Asn Asp Gln AlaTrp Leu Phe Asp Leu Asp Gly Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Arg GlnThr Val Asp Thr Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro Asp Leu Ile ProGln Val Gln Gln Met Leu Ser Gly Gln His Leu Arg Asn Pro Val Ile Val Asp GlyArg Leu His Val Thr Thr Ile Asp Gly Val Val Val Gly Phe Asp Lys Lys Ser ProThr Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu GluAla Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu GlnGly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile Val ThrAla Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly Met Ala Val Gly AlaAla Thr Pro Tyr Ile Tyr Asn Thr Ser Pro Arg Arg Arg Ser Ile Val Pro Glu GluIle Thr Ile Pro Thr Leu Ala Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Asn AlaThr Ile Val Ile Asp Arg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp AsnLys Val Asp Gln Asp Gly Gly Ala Lys Val Val Leu Gly Ser Glu Ile Pro Met LysVal Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly AsnVal Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Cys Gln Ile Asp IleLys Val Gly Asp Ala Glu Thr Met Leu Ala Ser Asp Phe Ser Glu Ile Phe Ser LysThr His Pro Leu Val Ala Ser Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val ThrLys His Lys Ser Asp Tyr Glu Asp Leu Trp Ala Ile Leu His Met Thr
实施例2：35s启动子+苏铁毒蛋白基因的植物表达载体的构建
线性化载体制备：使用限制性内切酶BstBI和MluI双酶切VM062-3*FLAG载体，使环状载体线性化。
带有同源臂的目标序列扩增：
SEQ ID NO.17：
以正向引物：
5’-gggcggtaccccgggttcgaaATGGTTATGGTAATTGACTATCCAAGTC-3’；
反向引物：
5’-cactagttaattaagacgcgtCTATGTCATGCGTAGAATGGCCC-3’为引物，对苏铁毒蛋白基因进行PCR扩增，扩增体系为：
KOD OneTM PCR Master Mix 25μL
上游引物(10μM each)1.5μL
下游引物(10μM each)1.5μL
模板2μL
灭菌水20μL
总体积50μL
PCR的程序为98℃5min；98℃30s，50℃30s，68℃3min，30个循环；然后68℃10min，4℃保存。
载体酶切产物与PCR产物的检测与回收：琼脂糖凝胶电泳检测PCR产物与载体酶切产物，使用Quick Gel Extraction Kit回收目的片段。
胶回收产物重组：使用-Basic Seamless Cloning and Assembly Kit进行同源重组，反应体系如下：
2×Basic Assembly Mix 5μL
酶切载体XμL
PCR产物YμL
灭菌水to 10μL
*10μL反应体系中，载体与各个***片段加入量建议均在0.01～0.25pmols，载体与各个***片段的最佳摩尔比为1：2。
轻轻混合，50℃反应15分钟。
转化方法：
1.在冰上融化Trans1-T1感受态细胞，静置2-5分钟，观察细胞是否已解冻，用手指轻弹管底1-2次。
2.在50μL细胞中加入2μL的重组产物，轻轻弹动离心管壁混匀后，在冰上放置30分钟。
3.42℃水浴中热激30秒，之后马上转至冰上冷却2分钟。
4.加入450μL常温无抗LB液体培养基，之后37℃摇床中250rpm培养1小时。
5. 5000rpm离心2分钟，收集菌体，留100μL液体重悬，将其均匀涂布于含有卡那霉素抗性的LB固体平板上，之后37℃培养箱过夜培养。
测序鉴定：经菌落PCR和质粒PCR筛选阳性克隆，送至上海生工生物工程有限公司测序分析，结果表明：成功得到了苏铁毒蛋白基因植物超表达载体VM062-Suit(见图2)。
本发明选用VM062-3*FLAG载体作为基础植物表达载体，由于35S启动子与3*FLAG之间酶切位点易发生移码突变，加上标签位于蛋白N端有利于蛋白表达，因此本发明采用3*FLAG标签之后的BstBI和MluI酶切位点，用于构建最终表达载体。载体序列如序列SEQ IDNO.18。
SEQ ID NO.18植物表达载体序列：
GAATTCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGCTAGAGCAGCTTGCCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCTGAAATCACCAGTCTCTCTCTACAAATCTATCTCTCTCGAGTCTACCATGGCTCAGATCAGGTCGATGGCTCAGGGCATTCAGACGCTGTCGCTCAACTCCTCTAATCTCTCCAAGACGCAGAAGGGGCCGCTCGTGTCGAACTCTCTCTTCTTCGGCAGCAAGAAGCTGACACAGATCTCTGCCAAGTCACTGGGGGTTTTCAAGAAGGACTCGGTGCTCCGGGTGGTCCGCAAGTCCAGCTTCCGCATCTCAGCTTCCGTCGCTACAGCTGAGGCTGAGGCCGTGATCGCTGAGGTCTCCACTCAGCTCAGCGAGGTTGTGGGCGTGATCGAGAGGCACCTGGAGCCAACCCTCCTGGCTGTCCATCTCTACGGGTCAGCGGTTGATGGTGGCCTGAAGCCCCACTCCGACATCGATCTCCTGGTTACAGTGACTGTCCGGCTGGACGAGACCACGAGGAGGGCTCTCATTAACGATCTCCTGGAGACCAGCGCTTCGCCAGGCGAGTCCGAGATCCTCAGGGCGGTTGAGGTGACGATTGTCGTTCATGACGATATCATTCCATGGAGGTACCCAGCTAAGAGGGAGCTCCAGTTCGGCGAGTGGCAGCGCAATGACATCCTGGCCGGGATTTTCGAGCCAGCGACAATCGACATTGATCTGGCTATCCTCCTGACTAAGGCTAGGGAGCACTCCGTCGCTCTGGTTGGCCCTGCTGCTGAGGAGCTCTTCGACCCAGTCCCTGAGCAGGATCTCTTCGAGGCCCTGAACGAGACCCTCACGCTGTGGAATTCTCCGCCCGACTGGGCTGGCGATGAGAGGAATGTGGTCCTCACCCTGTCGCGCATCTGGTACTCTGCTGTCACGGGGAAGATTGCTCCAAAGGACGTGGCTGCTGATTGGGCGATGGAGAGGCTGCCAGCTCAGTACCAGCCTGTGATCCTCGAGGCTAGGCAGGCTTACCTGGGCCAGGAGGAGGACAGGCTCGCGTCCCGGGCTGATCAGCTGGAGGAGTTCGTTCATTACGTCAAGGGCGAGATTACTAAGGTCGTTGGCAAGTGACTCGAGTTTCTCCATAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGTTCCTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATTTGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGTACTAAAATCCAGATCCCCCGAATTAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTGCCACCAGCCAGCCAACAGCTCCCCGACCGGCAGCTCGGCACAAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGTTGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCTGCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAGCGTTGCTGCCTGTGATCACCGCGGTTTCAAAATCGGCTCCGTCGATACTATGTTATACGCCAACTTTGAAAACAACTTTGAAAAAGCTGTTTTCTGGTATTTAAGGTTTTAGAATGCAAGGAACAGTGAATTGGAGTTCGTCTTGTTATAATTAGCTTCTTGGGGTATCTTTAAATACTGTAGAAAAGAGGAAGGAAATAATAAATGGCTAAAATGAGAATATCACCGGAATTGAAAAAACTGATCGAAAAATACCGCTGCGTAAAAGATACGGAAGGAATGTCTCCTGCTAAGGTATATAAGCTGGTGGGAGAAAATGAAAACCTATATTTAAAAATGACGGACAGCCGGTATAAAGGGACCACCTATGATGTGGAACGGGAAAAGGACATGATGCTATGGCTGGAAGGAAAGCTGCCTGTTCCAAAGGTCCTGCACTTTGAACGGCATGATGGCTGGAGCAATCTGCTCATGAGTGAGGCCGATGGCGTCCTTTGCTCGGAAGAGTATGAAGATGAACAAAGCCCTGAAAAGATTATCGAGCTGTATGCGGAGTGCATCAGGCTCTTTCACTCCATCGACATATCGGATTGTCCCTATACGAATAGCTTAGACAGCCGCTTAGCCGAATTGGATTACTTACTGAATAACGATCTGGCCGATGTGGATTGCGAAAACTGGGAAGAAGACACTCCATTTAAAGATCCGCGCGAGCTGTATGATTTTTTAAAGACGGAAAAGCCCGAAGAGGAACTTGTCTTTTCCCACGGCGACCTGGGAGACAGCAACATCTTTGTGAAAGATGGCAAAGTAAGTGGCTTTATTGATCTTGGGAGAAGCGGCAGGGCGGACAAGTGGTATGACATTGCCTTCTGCGTCCGGTCGATCAGGGAGGATATCGGGGAAGAACAGTATGTCGAGCTATTTTTTGACTTACTGGGGATCAAGCCTGATTGGGAGAAAATAAAATATTATATTTTACTGGATGAATTGTTTTAGTACCTAGAATGCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGGGTGCCTTGATGTGGGCGCCGGCGGTCGAGTGGCGACGGCGCGGCTTGTCCGCGCCCTGGTAGATTGCCTGGCCGTAGGCCAGCCATTTTTGAGCGGCCAGCGGCCGCGATAGGCCGACGCGAAGCGGCGGGGCGTAGGGAGCGCAGCGACCGAAGGGTAGGCGCTTTTTGCAGCTCTTCGGCTGTGCGCTGGCCAGACAGTTATGCACAGGCCAGGCGGGTTTTAAGAGTTTTAATAAGTTTTAAAGAGTTTTAGGCGGAAAAATCGCCTTTTTTCTCTTTTATATCAGTCACTTACATGTGTGACCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGGGTTCCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGTGCTATCCACAGGAAAGAGACCTTTTCGACCTTTTTCCCCTGCTAGGGCAATTTGCCCTAGCATCTGCTCCGTACATTAGGAACCGGCGGATGCTTCGCCCTCGATCAGGTTGCGGTAGCGCATGACTAGGATCGGGCCAGCCTGCCCCGCCTCCTCCTTCAAATCGTACTCCGGCAGGTCATTTGACCCGATCAGCTTGCGCACGGTGAAACAGAACTTCTTGAACTCTCCGGCGCTGCCACTGCGTTCGTAGATCGTCTTGAACAACCATCTGGCTTCTGCCTTGCCTGCGGCGCGGCGTGCCAGGCGGTAGAGAAAACGGCCGATGCCGGGATCGATCAAAAAGTAATCGGGGTGAACCGTCAGCACGTCCGGGTTCTTGCCTTCTGTGATCTCGCGGTACATCCAATCAGCTAGCTCGATCTCGATGTACTCCGGCCGCCCGGTTTCGCTCTTTACGATCTTGTAGCGGCTAATCAAGGCTTCACCCTCGGATACCGTCACCAGGCGGCCGTTCTTGGCCTTCTTCGTACGCTGCATGGCAACGTGCGTGGTGTTTAACCGAATGCAGGTTTCTACCAGGTCGTCTTTCTGCTTTCCGCCATCGGCTCGCCGGCAGAACTTGAGTACGTCCGCAACGTGTGGACGGAACACGCGGCCGGGCTTGTCTCCCTTCCCTTCCCGGTATCGGTTCATGGATTCGGTTAGATGGGAAACCGCCATCAGTACCAGGTCGTAATCCCACACACTGGCCATGCCGGCCGGCCCTGCGGAAACCTCTACGTGCCCGTCTGGAAGCTCGTAGCGGATCACCTCGCCAGCTCGTCGGTCACGCTTCGACAGACGGAAAACGGCCACGTCCATGATGCTGCGACTATCGCGGGTGCCCACGTCATAGAGCATCGGAACGAAAAAATCTGGTTGCTCGTCGCCCTTGGGCGGCTTCCTAATCGACGGCGCACCGGCTGCCGGCGGTTGCCGGGATTCTTTGCGGATTCGATCAGCGGCCGCTTGCCACGATTCACCGGGGCGTGCTTCTGCCTCGATGCGTTGCCGCTGGGCGGCCTGCGCGGCCTTCAACTTCTCCACCAGGTCATCACCCAGCGCCGCGCCGATTTGTACCGGGCCGGATGGTTTGCGACCGTCACGCCGATTCCTCGGGCTTGGGGGTTCCAGTGCCATTGCAGGGCCGGCAGACAACCCAGCCGCTTACGCCTGGCCAACCGCCCGTTCCTCCACACATGGGGCATTCCACGGCGTCGGTGCCTGGTTGTTCTTGATTTTCCATGCCGCCTCCTTTAGCCGCTAAAATTCATCTACTCATTTATTCATTTGCTCATTTACTCTGGTAGCTGCGCGATGTATTCAGATAGCAGCTCGGTAATGGTCTTGCCTTGGCGTACCGCGTACATCTTCAGCTTGGTGTGATCCTCCGCCGGCAACTGAAAGTTGACCCGCTTCATGGCTGGCGTGTCTGCCAGGCTGGCCAACGTTGCAGCCTTGCTGCTGCGTGCGCTCGGACGGCCGGCACTTAGCGTGTTTGTGCTTTTGCTCATTTTCTCTTTACCTCATTAACTCAAATGAGTTTTGATTTAATTTCAGCGGCCAGCGCCTGGACCTCGCGGGCAGCGTCGCCCTCGGGTTCTGATTCAAGAACGGTTGTGCCGGCGGCGGCAGTGCCTGGGTAGCTCACGCGCTGCGTGATACGGGACTCAAGAATGGGCAGCTCGTACCCGGCCAGCGCCTCGGCAACCTCACCGCCGATGCGCGTGCCTTTGATCGCCCGCGACACGACAAAGGCCGCTTGTAGCCTTCCATCCGTGACCTCAATGCGCTGCTTAACCAGCTCCACCAGGTCGGCGGTGGCCCATATGTCGTAAGGGCTTGGCTGCACCGGAATCAGCACGAAGTCGGCTGCCTTGATCGCGGACACAGCCAAGTCCGCCGCCTGGGGCGCTCCGTCGATCACTACGAAGTCGCGCCGGCCGATGGCCTTCACGTCGCGGTCAATCGTCGGGCGGTCGATGCCGACAACGGTTAGCGGTTGATCTTCCCGCACGGCCGCCCAATCGCGGGCACTGCCCTGGGGATCGGAATCGACTAACAGAACATCGGCCCCGGCGAGTTGCAGGGCGCGGGCTAGATGGGTTGCGATGGTCGTCTTGCCTGACCCGCCTTTCTGGTTAAGTACAGCGATAACCTTCATGCGTTCCCCTTGCGTATTTGTTTATTTACTCATCGCATCATATACGCAGCGACCGCATGACGCAAGCTGTTTTACTCAAATACACATCACCTTTTTAGACGGCGGCGCTCGGTTTCTTCAGCGGCCAAGCTGGCCGGCCAGGCCGCCAGCTTGGCATCAGACAAACCGGCCAGGATTTCATGCAGCCGCACGGTTGAGACGTGCGCGGGCGGCTCGAACACGTACCCGGCCGCGATCATCTCCGCCTCGATCTCTTCGGTAATGAAAAACGGTTCGTCCTGGCCGTCCTGGTGCGGTTTCATGCTTGTTCCTCTTGGCGTTCATTCTCGGCGGCCGCCAGGGCGTCGGCCTCGGTCAATGCGTCCTCACGGAAGGCACCGCGCCGCCTGGCCTCGGTGGGCGTCACTTCCTCGCTGCGCTCAAGTGCGCGGTACAGGGTCGAGCGATGCACGCCAAGCAGTGCAGCCGCCTCTTTCACGGTGCGGCCTTCCTGGTCGATCAGCTCGCGGGCGTGCGCGATCTGTGCCGGGGTGAGGGTAGGGCGGGGGCCAAACTTCACGCCTCGGGCCTTGGCGGCCTCGCGCCCGCTCCGGGTGCGGTCGATGATTAGGGAACGCTCGAACTCGGCAATGCCGGCGAACACGGTCAACACCATGCGGCCGGCCGGCGTGGTGGTGTCGGCCCACGGCTCTGCCAGGCTACGCAGGCCCGCGCCGGCCTCCTGGATGCGCTCGGCAATGTCCAGTAGGTCGCGGGTGCTGCGGGCCAGGCGGTCTAGCCTGGTCACTGTCACAACGTCGCCAGGGCGTAGGTGGTCAAGCATCCTGGCCAGCTCCGGGCGGTCGCGCCTGGTGCCGGTGATCTTCTCGGAAAACAGCTTGGTGCAGCCGGCCGCGTGCAGTTCGGCCCGTTGGTTGGTCAAGTCCTGGTCGTCGGTGCTGACGCGGGCATAGCCCAGCAGGCCAGCGGCGGCGCTCTTGTTCATGGCGTAATGTCTCCGGTTCTAGTCGCAAGTATTCTACTTTATGCGACTAAAACACGCGACAAGAAAACGCCAGGAAAAGGGCAGGGCGGCAGCCTGTCGCGTAACTTAGGACTTGTGCGACATGTCGTTTTCAGAAGACGGCTGCACTGAACGTCAGAAGCCGACTGCACTATAGCAGCGGAGGGGTTGGATCAAAGTACTTTGATCCCGAGGGGAACCCTGTGGTTGGCATGCACATACAAATGGACGAACGGATAAACCTTTTCACGCCCTTTTAAATATCCGTTATTCTAATAAACGCTCTTTTCTCTTAGGTTTACCCGCCAATATATCCTGTCAAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCCAAGCTCAAGCTGCTCTAGCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTAAGCTTGCATGCCTGCAGGTCCCCAGATTAGCCTTTTCAATTTCAGAAAGAATGCTAACCCACAGATGGTTAGAGAGGCTTACGCAGCAGGTCTCATCAAGACGATCTACCCGAGCAATAATCTCCAGGAAATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCACAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAAAAGGTAGTTCCCACTGAATCAAAGGCCATGGAGTCAAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGAACACGGGGGACTCTAGAGGATCGCGAGGCGCGCCGTCGACATGGACTACAAGGACGACGATGATAAGGGCATGGACTACAAGGACGACGATGATAAGGGCATGGACTACAAGGACGACGATGATAAGGGCGGTACCCCGGGttcgaaATGGTTATGGTAATTGACTATCCAAGTCTCAGAGCTTCGTTATATGCCACTAAGAGTGCGGTCCCATGTCGCCCGCTTCCGGGCAATCCAGAGCGGGTGTCGCGCCGGGTATCACGCAGAGGCGCGGGAGAGATCGGGTCCCATGCAGATTTCTGGATCTCCCGCGCCAAGGCATCCGCCATCCGGATTTTCCCGCGTCAAGAGGGTCCGCTCCGTGAATCCCGGAGAAGATTCTATCTTCTCCGCGAAGCGGTGTTCACCGGCCGCAGGACCGCCTGGGCCGCTAATCCAGCGGCCCAACGGGCAGCATGGGACCGCAAGAGATCCCCTTTCTTCGGAAGGTCTTCCGATGATATTCATTCATTCAGCAAGAATCCAGAAGAATTCTTGACTCAAAACACTCTCAGCTGCAGTTTATTAACTCGCACCGCTCAGACCGTCACAAAAAGCCCAGTATTGCCCGCCCAAGGTTGGGTCAAAGTGGGTCGTGTGCCAGGAAGAGGAGACGCTCTCTTTGTTGAGCTGGTTGCCGAGAAATCAGATGAGACAGTGAAGGCGTACTTACTTGGCTACAATGGTAAGAACGACTCCCCCGAGCCGGCATACGTTGATATTCCAAAGAGGGGCGCCGAGAACGGAACATTTTTGTTTACCGTGGATCTCTCGGGCTGCTCCGTTGTTGTGACACAGTTGGACGAGCAGACATACCGTGTTTTCCACGACAAACGGGTGAACAGCTCCATTCTGTACGACAACGTCGTAATGTATGTGGATTTCAATCACTACCGCTATCCCAGAGCCCACGGACACGGGAGAGATACGGGACAAGCGGTCGTCTGTATGCAATTCGCCGACGGAGTGTGGAAATTGTTTCTCCAGCGGCACGAGACGGCGAATTCTCTCCCAAAGATATGGTCCCGTGGCGATGGCAAACCGGAGATTTTCAGCGACGCCGAACCCCGTCCACATATGGAAGATGGCATAAAGGAATTTCTCAATCGGCGTCGAGCTTTGCAAGACGAATTGAAGCACTTCGCCAACACGCTTGAAGTGGACCCGGACATAGTGTCCAACGCTGTCGACGGCGAATACGAAGGCGGCCAGTTTTCCAGGAACGACGATGCAATTCGTGGATGGAACGAATTACGTGCAAGCATGAGACAGAAAATGGCTGAAAGGAAGAAACTGTTGCAAACAAAGAAAGGTATGCTAGACTCATTCAACAACCCGGATCACCCCCTCTATAAATTTCGCAAACAAGTGCTCGACGAGCTCAAGGAAATGAGTGCATACGACAACACCTTGGAGGCATCCGAGATTGCGGACCGTACTTGGCTGTGGTTGGAGATCAAAAAAGCCAAAGGTACGAATGCAGTGGTAAACAGTGGAGCAGCCCCGAAGCAGCCGAGTCTTACCGTTGGTGAACGCTACGCAGAGATGGAGGCGGCCCTTTGGATGCGTAGCGACACAGCGGCGTTTGAGCAAGGGTATGAATCTTATCAAACCGTTCGCGTGCCGGGTTACAATGAAGATATGACATCCATTGCAATGAAGAAGTTGTTCGTTGAGAGCAGTCTGACTGACGAGCAGCTGGGCGCCTTGTGCAAGCGTATATCCATTGCTAGCGATCAAGAGAACCATCTTCTGACACAGAAGACGCAGGAGATTGAGAGCACTTTCAAACAATTTTCAGATGGCAGCTTTTTGGAGTCGGCGCCTCAGGATTTCTTCCTGAGACGAGTCGGTGATAAATCTGGAGGACGCTGCTATCCTCTTGTAAGGGTAATGGCCGTCGCACTGTCTGAAGGAGAAGACGTTGCCCAGAATTTTATGGAGAACCTGAGGACATTGTCTAGGAATCCAGAGTATTCAGGCGAATCGGCAAGTTCACAAGCCACAAATGCAGGGTTGTTCAGAGATGCCCTGGCCGGCCTGCACGCGAATGTGGAAGCCATTGCCGCATCTGAGAAACTACCTGCTGTGTTTGGACTGCCCGATGTTGCTACAATGTTGAGCCAAGCGAGCGAAAGCAAGGTTTACGCTTTGAATACCGAAACACACAGCATGCTGGTTGCGGTGATTGTAGACAAACAGAGTCTGGCGCGCAAGTATTACTTCTATGATCCCAACTTTCTCATGGCCGGTTTCTCCGACATAACTCGTTTCTCGGAAGCCCTCAAGTATCACTTTGTGAATCTCCAGTTCGCCGGAAACTACAAAGCGTTTGGCACAGCGGAGGCTCCCCAGTTTAACCTGGTCCTTATTGATACGACCGCCATGGAGGATGCGCATGTTGACGGAGGGATCACCGTGAAACAATTGAGCGAGGGCGTTGATCTTACTAATGTCTTTGACAGCACCAAAAAGGCTCGTGTGGTAACTGAGGCAGCAGCTCGCTTGGCTGAAGACCAACAACTGCGCACTGGCCTCACCATAATAGAGGCTGACCAGTGGGCAAACAGGTGGCAAGATGCCGTCATACGATTGAAACTCAAGGAAGGATTAAGTGGAGAGTGGATGCCATTGCTGGAGAAAATGCAAGAAAACGCAGATGGTTCTTATCAGATTGATTTCATCAACACCAAGAATACTGGGGAAATCCGTGGGGTTTCCACTAGAGATGCGACGTTCAAAGAATTCAAATCCTACCTGGATAAAAATTTAGACGTATTGCATAAGGATTTCATCTTTCAGGATGGAGTTTTCAAACCACTGGAGGGAGAGCCCATTGACGGGTTGAATGCAATGTTCGCGGTCCAAATGATTATAGATTGGTTCAACCGGGGGACTAATGCTGAGGAAGATGCGGCCTCAGGATCCACCAATGCCCAACTTTCCCTAGCGATTAAAGTGCACAGCTACATTAACATGGCCCAAGTAGCGCATACTGCAGTTACAGATGTTGCCAAGGTATACAACCTTGTAAAGTCAGCACTGTATGGCGAGCAGGTAGCTGGGGAGACATCGCTGTTCACATTTGGGAAGGCGTTGAAATTTGCAGCAACAGAAGGGTTGGGAGTCTTACTAGGGGCGTCCTTTGTGATCTTGGATGCCTACCAGCTGTCCCTCGTGGAAAACGATGTGCAGAAGGCCATTATCGGCACACAACTAGCCTTTGATTCCGCAAGCTTTCTAGCGGGTATTGGTGCAATTGTTGCTGGAGCATCGGGCGTGGGCATGATTTTGGGCGGAGCTGGCGTGGTATTGGGAGGCCTGGGAGTTGGCTTTGGAGCCTTAGCTCAGGCATTCGGGGAAATCACAGCAGACGCCCAACTGGTGGGCAAGTACTTCGCACTCTTGGACGAGGCATACCGCGGTGGCGGATACAGATACGAATCTAAAGACAAGGCATTGATGCCTCTTGCAGGAGCAGTCATCGACAAAATACGACTGGCCGACAAACATCTCCAAGTTCATTTCGACAGCCAATTCATATATCGAACCCATCATGGCAGCACAGGATCCGGCAAAATTAATTACTTCTTCTGGGCTGGCGACATGCCGCAGATGGTCCACGATCGCTCCCAAGCTATCAATGTTCGAGAGCAAATTGGGTATAAGAACGATAATGCTACTTTGGAGCTACCGGAGGATGCCTCTGATCTGATCCTGCCTGCCACGCCCAAGTCATTTATCACTTACCGATACGAGATTCTACCATTCGCTACTTCCCGCCACGATACGGGTTTCGATGTCATTAGACGCCTGGAAGAAGACTACAGATTCGATTACGATTTCTACATCTTCCCATCTGAATACACAATCAGAACCATCAAACACGAGTACGTAGAAACAAAGGTGGCAGTAGTCCTGGATAGGCGCTCTTTGCGACTGGGAGCACCTGAGCTTCTCAAGGAGCAACATGGCTATTTGATCTATGAGATCGAGGGGACCGGAGGACAATACGTTATATGTCTTAACCAGGGATCCACTGTCACACTGAAGACCACCGGATCAGAGGCAAATAAAACATTATGGATACTTGATGCCCGACAACTCATTGCAGGCAACGATTCAATCACGGTCTCTCAAGATAGCATCACCATCGGCAGCACAACAGTCCATCTCCGTAACCAAGATTTCGATTGCCTACAGATCGTCATGAGTCGGGAGGAGCTTCTCCAAGTGGACTTGGCGTCTGGAGAAGCCCGCGTGTTTTCAGAAGATGCCAGTAAATGGCTCGACACCAAAGAAACAATCCATGAACATCTCCATAAGTTGGCTGAAGAACACAGGTTGCATGGCCAGTTTGTAGTGGTGAACAATTATTATCGTGGAGTGGGCCGTGCCTACTATGATGCTGCTCACGATCGGATGTTGTATACAGATGTGGAAGATGAAACTCTGACTCGGGATGCTGTTCTTGCCGGCGTTACAGGCGAAGATGCCTACTTCTACAATGTAAAGCAGAATGCCCTGTGGCGAGTGAATGCAGGCACACACCAAGTAATAACCAAATACTATCCGACGTTACTGGAGGACCCTGACAGCAACATGGCAAGAGTTGAGCAGCATGGCAACATCGTCGTGATGGCCATGAGCAGGCTCAACCACCCGGACAAGGGAGTAAATGGAATCATCTATCTCGTTCATCAAGAAAGCATCGTGCTTACTGCTGTGATCGGCAATTCAGAGATGCTCAAACGTCTTCGCCAAACGAGTACAATTAGCTTAACAGAATTGCGAGGTGTCAATGACATGGCTCTGGTTTCCACGATGGACAGATTACCCGGCCAAAAGGTTGTCGCTACCAACGCTGAGTTTGTGACACTTCTGGCTAAGGATGAGCAAGGAGCCAATATCAAGGAGCAGACGGTCTATTGCCAACAAGGTACGGGCCAGACCAACGATACATCTATTGAAAGCATCCCGATTCCAGGGCTCTCTAATCTTTTAACCGTGTACGGTGAAGTATTCGCTACAACTAATGATGGTCGCATCCTTCGCCTGCTTGGGGACGGCTCAACATGTCTCGAGGGCATCAATGAGCATTGGCTCATCAAGCATCCAAAATGGTGGCAGGACTTGGCTACCTTGGAGGCAGAGAAAGCCACGACAGTTGCAGTATTCGGTGTTAGAGACCTACAAAATGCAGTGGTGCCAATTTGGTACCACAACGGAAAGATCGCGGTGGCCTCATCAAAACTGTATGGCAAACAACTGCAATTTTTGGGGTTTAACAGCAACGGTGAAGCTTGGTTGTTCGACTTGGACGACGGCATTCTGTATAGCCAGCCGCTTGTGGATAGCTTTCTGTATGCAATCTTTAGTTTGGACGGGAAGCTTATTGCACCAATCTTTATTCCACAAGTGGAGCAAATGCTCAGCGGCCAGCATCTCAGAAACCCTGTCATCGTGGACGGCCGTTTGCATGTGACTACCATAGACGGTGTTGTTGTTGGATTTGACAAGAAGTCGCCAACTCTGGTTGCAGTGAACAAGGCCTGGCAGATATCTCGTCAGAACCGATTAGAAGAGGCCCTCGGAAACCTGCTGTCCAATTGGTCTGATCACAATGATGCGATTGTGTTGCAGGGTGATCCAGAATCAGCGCCGGCATGGTATTATGTTCCATCAAGAGAAATAGTGACCGCCAATGGTCTAACATGGGCGGATCATCCTCTATGGCTAGGAATGGCTGTCGGTGCTGCAACGCCATACATCTACAGCACATCCCGACGCCGTGTGTATGTTGTGGAGAGCAAAAACATGGCCAAAGACAAAGGAGAATTCGCAGCAGCACATTGCTTTGCAAAATCACTTGTGCTACAGAGCATCGTTCCCGAGGAAATAACGATTCCGACGCTGGCTGGCATCGATTTCGCACTCGTTGCAGCGACAAATGCAACCATTGTAATAGACCGAGACAATTGGAAGCATTACAAGGCCATTGTGCTCGACAACAAGGTGGATCAGGACGGTGGCGCTAAGGTGGTAGTCGGCAGTGAGATTCCCATGAAAGTTCTGGTGCGCAAATTTCAGGGAAATCTGGAGTTCTTCGATCCAAACAATGGTAATGTTGTTATACTCCAGAATGTGTTGAAGGGAGACGGAGCTTGTTGCCAGATAGAGATCAAAGTAGGCGATGCTGAGACTATGCTTGTATCCGATTTCTCGGAGATATTCTCAAAGACGCATCCTCTGGTGGCAAGCCAAGTATTTGAGCTTGGGGATGTGATTGCAGTCACAAAACACAAATCGGACTATGCGGATCTGTGGGCCATTCTACGCATGACATAGacgcgtCTTAATTAACTAGTGAGCTCGAATTTCCCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGG
实施例3：转基因棉花株系获得(见图3)
将实施例2所得植物过表达载体VM062-Suit送江苏未米生物科技有限公司进行转化，转化背景为棉花品种“百棉1号”。从公司获得的T0代转基因棉花株系，栽培收获T1代，SEQ ID NO.19：以正向引物：5’-GACTACAAGGACGACGATGATAAGG-3’；反向引物：5’-GTATGCACTCATTTCCTTGAGCTCG-3’为引物，对苏铁毒蛋白基因基因特异1400bp片段进行PCR扩增，筛选T1阳性植株并收获种子。结果如图4所示，确定目的基因未发生遗传分离丢失，最终获得含有苏铁毒蛋白基因稳定遗传的阳性纯合转基因棉花。
实施例4：转基因棉花植株的苏铁毒蛋白基因蛋白水平表达检测
取转基因棉花纯合株系叶片，提取叶片总蛋白，用于Western Blot检测。具体步骤：
1、取新鲜样品1～2g；
2、把样品放在研钵中用液氮研磨，研磨后置于10mL试管中，并加入蛋白提取液(0.01M PBS pH＝8，1mM PMSF，2mM DTT)4℃摇匀30min。
3、12000rpm 4℃离心20min。
4、提取上清液，样品制备完成。
5、取上清加入Loading buffer，然后98℃煮5～10分钟，后用SDS-聚丙烯酰胺凝胶电泳法测定蛋白质表达情况，见图5。
实施例5：苏铁毒蛋白转基因棉花植株的抗虫性检测
取龄期一致的三龄第二天的小菜蛾(平均体重1.33mg/头)。每头注射2.03μg苏铁蛋白，对照组注射等体积(207nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果见表1。
取龄期一致的二龄第二天的棉铃虫(平均体重是2.06mg/头)。每头注射3.38μg苏铁蛋白，对照组注射等体积(345nL)的磷酸盐缓冲液(PBS)。设三次重复，每个重复注射30头幼虫。注射后24h统计死亡率，结果见表1。
表1 注射苏铁蛋白后小菜蛾和棉铃虫幼虫的死亡率
取龄期一致的二龄草地贪夜蛾(平均体重1.01mg/头)用于草地贪夜蛾的取食实验。实验前，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫。25℃培养4天统计死亡率，取食结果见图6，死亡率统计见表2。
取龄期一致的二龄棉铃虫(平均体重3.9mg/头)用于棉铃虫的取食实验。实验前，统一进行8h饥饿实验，而后以转基因棉花纯合株系叶片为实验组，转基因受体材料“百棉1号”和陆地棉遗传标准系TM-1为对照组。设三次重复，每个重复培养18头幼虫。25℃培养4天统计死亡率，取食结果见图6，死亡率统计见表2。
表2 草地贪夜蛾和棉铃虫幼虫死亡率
二、应用实施例。为了证明本发明的技术方案的创造性和技术价值，该部分是对权利要求技术方案进行具体产品上或相关技术上的应用实施例。
本发明提供了一种来源于苏铁的毒蛋白基因，该基因长6240bp，编码2080个氨基酸，将其导入到棉花中，使转基因棉花获得草地贪夜蛾和棉铃虫抗性，且后代能够稳定遗传。详细实施例见：
实施例1.克隆苏铁毒蛋白基因。
实施例2.35s启动子+苏铁毒蛋白基因的植物表达载体的构建。
实施例3.转基因棉花株系获得。
实施例4.转基因棉花植株的苏铁毒蛋白基因蛋白水平表达检测。
实施例5.苏铁毒蛋白转基因棉花植株的抗虫性检测。
三、实施例相关效果的证据。本发明实施例在研发或者使用过程中取得了一些积极效果，和现有技术相比的确具备很大的优势，下面内容结合试验过程的数据、图表等进行描述。
本发明提供了来源于苏铁的毒蛋白基因，该基因是本发明首次发现，来源于苏铁的一种抗虫基因，将其导入到棉花中，根据苏铁毒蛋白基因的表达情况(图4)，说明该基因已成功导入棉花，并能稳定遗传；根据苏铁毒蛋白基因的表达情况(图5)，说明该基因所编码的蛋白已在棉花中成功表达；根据转基因植株的抗虫实验结果(图6，表1，表2)，发现转基因棉花对草地贪夜蛾抗性达到61.11％，对棉铃虫抗性达59.26％。这些结果与其它研究结果相比，说明本发明单价转基因棉花所获得的抗性与他人研究的双价转基因棉花具有类似效应。
以上所述，仅为本发明的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，都应涵盖在本发明的保护范围之内。
序列表
<110> 河南大学
深圳华大生命科学研究院
深圳市仙湖植物园（深圳市园林研究中心）
<120> 一种抗虫性的苏铁毒蛋白基因、表达载体及应用
<160> 19
<170> SIPOSequenceListing 1.0
<210> 1
<211> 6240
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
atggttatgg taattgacta tccaagtctc agagcttcgt tatatgccac taagagtgcg 60
gtcccatgtc gcccgcttcc gggcaatcca gagcgggtgt cgcgccgggt atcacgcaga 120
ggcgcgggag agatcgggtc ccatgcagat ttctggatct cccgcgccaa ggcatccgcc 180
atccggattt tcccgcgtca agagggtccg ctccgtgaat cccggagaag attctatctt 240
ctccgcgaag cggtgttcac cggccgcagg accgcctggg ccgctaatcc agcggcccaa 300
cgggcagcat gggaccgcaa gagatcccct ttcttcggaa ggtcttccga tgatattcat 360
tcattcagca agaatccaga agaattcttg actcaaaaca ctctcagctg cagtttatta 420
actcgcaccg ctcagaccgt cacaaaaagc ccagtattgc ccgcccaagg ttgggtcaaa 480
gtgggtcgtg tgccaggaag aggagacgct ctctttgttg agctggttgc cgagaaatca 540
gatgagacag tgaaggcgta cttacttggc tacaatggta agaacgactc ccccgagccg 600
gcatacgttg atattccaaa gaggggcgcc gagaacggaa catttttgtt taccgtggat 660
ctctcgggct gctccgttgt tgtgacacag ttggacgagc agacataccg tgttttccac 720
gacaaacggg tgaacagctc cattctgtac gacaacgtcg taatgtatgt ggatttcaat 780
cactaccgct atcccagagc ccacggacac gggagagata cgggacaagc ggtcgtctgt 840
atgcaattcg ccgacggagt gtggaaattg tttctccagc ggcacgagac ggcgaattct 900
ctcccaaaga tatggtcccg tggcgatggc aaaccggaga ttttcagcga cgccgaaccc 960
cgtccacata tggaagatgg cataaaggaa tttctcaatc ggcgtcgagc tttgcaagac 1020
gaattgaagc acttcgccaa cacgcttgaa gtggacccgg acatagtgtc caacgctgtc 1080
gacggcgaat acgaaggcgg ccagttttcc aggaacgacg atgcaattcg tggatggaac 1140
gaattacgtg caagcatgag acagaaaatg gctgaaagga agaaactgtt gcaaacaaag 1200
aaaggtatgc tagactcatt caacaacccg gatcaccccc tctataaatt tcgcaaacaa 1260
gtgctcgacg agctcaagga aatgagtgca tacgacaaca ccttggaggc atccgagatt 1320
gcggaccgta cttggctgtg gttggagatc aaaaaagcca aaggtacgaa tgcagtggta 1380
aacagtggag cagccccgaa gcagccgagt cttaccgttg gtgaacgcta cgcagagatg 1440
gaggcggccc tttggatgcg tagcgacaca gcggcgtttg agcaagggta tgaatcttat 1500
caaaccgttc gcgtgccggg ttacaatgaa gatatgacat ccattgcaat gaagaagttg 1560
ttcgttgaga gcagtctgac tgacgagcag ctgggcgcct tgtgcaagcg tatatccatt 1620
gctagcgatc aagagaacca tcttctgaca cagaagacgc aggagattga gagcactttc 1680
aaacaatttt cagatggcag ctttttggag tcggcgcctc aggatttctt cctgagacga 1740
gtcggtgata aatctggagg acgctgctat cctcttgtaa gggtaatggc cgtcgcactg 1800
tctgaaggag aagacgttgc ccagaatttt atggagaacc tgaggacatt gtctaggaat 1860
ccagagtatt caggcgaatc ggcaagttca caagccacaa atgcagggtt gttcagagat 1920
gccctggccg gcctgcacgc gaatgtggaa gccattgccg catctgagaa actacctgct 1980
gtgtttggac tgcccgatgt tgctacaatg ttgagccaag cgagcgaaag caaggtttac 2040
gctttgaata ccgaaacaca cagcatgctg gttgcggtga ttgtagacaa acagagtctg 2100
gcgcgcaagt attacttcta tgatcccaac tttctcatgg ccggtttctc cgacataact 2160
cgtttctcgg aagccctcaa gtatcacttt gtgaatctcc agttcgccgg aaactacaaa 2220
gcgtttggca cagcggaggc tccccagttt aacctggtcc ttattgatac gaccgccatg 2280
gaggatgcgc atgttgacgg agggatcacc gtgaaacaat tgagcgaggg cgttgatctt 2340
actaatgtct ttgacagcac caaaaaggct cgtgtggtaa ctgaggcagc agctcgcttg 2400
gctgaagacc aacaactgcg cactggcctc accataatag aggctgacca gtgggcaaac 2460
aggtggcaag atgccgtcat acgattgaaa ctcaaggaag gattaagtgg agagtggatg 2520
ccattgctgg agaaaatgca agaaaacgca gatggttctt atcagattga tttcatcaac 2580
accaagaata ctggggaaat ccgtggggtt tccactagag atgcgacgtt caaagaattc 2640
aaatcctacc tggataaaaa tttagacgta ttgcataagg atttcatctt tcaggatgga 2700
gttttcaaac cactggaggg agagcccatt gacgggttga atgcaatgtt cgcggtccaa 2760
atgattatag attggttcaa ccgggggact aatgctgagg aagatgcggc ctcaggatcc 2820
accaatgccc aactttccct agcgattaaa gtgcacagct acattaacat ggcccaagta 2880
gcgcatactg cagttacaga tgttgccaag gtatacaacc ttgtaaagtc agcactgtat 2940
ggcgagcagg tagctgggga gacatcgctg ttcacatttg ggaaggcgtt gaaatttgca 3000
gcaacagaag ggttgggagt cttactaggg gcgtcctttg tgatcttgga tgcctaccag 3060
ctgtccctcg tggaaaacga tgtgcagaag gccattatcg gcacacaact agcctttgat 3120
tccgcaagct ttctagcggg tattggtgca attgttgctg gagcatcggg cgtgggcatg 3180
attttgggcg gagctggcgt ggtattggga ggcctgggag ttggctttgg agccttagct 3240
caggcattcg gggaaatcac agcagacgcc caactggtgg gcaagtactt cgcactcttg 3300
gacgaggcat accgcggtgg cggatacaga tacgaatcta aagacaaggc attgatgcct 3360
cttgcaggag cagtcatcga caaaatacga ctggccgaca aacatctcca agttcatttc 3420
gacagccaat tcatatatcg aacccatcat ggcagcacag gatccggcaa aattaattac 3480
ttcttctggg ctggcgacat gccgcagatg gtccacgatc gctcccaagc tatcaatgtt 3540
cgagagcaaa ttgggtataa gaacgataat gctactttgg agctaccgga ggatgcctct 3600
gatctgatcc tgcctgccac gcccaagtca tttatcactt accgatacga gattctacca 3660
ttcgctactt cccgccacga tacgggtttc gatgtcatta gacgcctgga agaagactac 3720
agattcgatt acgatttcta catcttccca tctgaataca caatcagaac catcaaacac 3780
gagtacgtag aaacaaaggt ggcagtagtc ctggataggc gctctttgcg actgggagca 3840
cctgagcttc tcaaggagca acatggctat ttgatctatg agatcgaggg gaccggagga 3900
caatacgtta tatgtcttaa ccagggatcc actgtcacac tgaagaccac cggatcagag 3960
gcaaataaaa cattatggat acttgatgcc cgacaactca ttgcaggcaa cgattcaatc 4020
acggtctctc aagatagcat caccatcggc agcacaacag tccatctccg taaccaagat 4080
ttcgattgcc tacagatcgt catgagtcgg gaggagcttc tccaagtgga cttggcgtct 4140
ggagaagccc gcgtgttttc agaagatgcc agtaaatggc tcgacaccaa agaaacaatc 4200
catgaacatc tccataagtt ggctgaagaa cacaggttgc atggccagtt tgtagtggtg 4260
aacaattatt atcgtggagt gggccgtgcc tactatgatg ctgctcacga tcggatgttg 4320
tatacagatg tggaagatga aactctgact cgggatgctg ttcttgccgg cgttacaggc 4380
gaagatgcct acttctacaa tgtaaagcag aatgccctgt ggcgagtgaa tgcaggcaca 4440
caccaagtaa taaccaaata ctatccgacg ttactggagg accctgacag caacatggca 4500
agagttgagc agcatggcaa catcgtcgtg atggccatga gcaggctcaa ccacccggac 4560
aagggagtaa atggaatcat ctatctcgtt catcaagaaa gcatcgtgct tactgctgtg 4620
atcggcaatt cagagatgct caaacgtctt cgccaaacga gtacaattag cttaacagaa 4680
ttgcgaggtg tcaatgacat ggctctggtt tccacgatgg acagattacc cggccaaaag 4740
gttgtcgcta ccaacgctga gtttgtgaca cttctggcta aggatgagca aggagccaat 4800
atcaaggagc agacggtcta ttgccaacaa ggtacgggcc agaccaacga tacatctatt 4860
gaaagcatcc cgattccagg gctctctaat cttttaaccg tgtacggtga agtattcgct 4920
acaactaatg atggtcgcat ccttcgcctg cttggggacg gctcaacatg tctcgagggc 4980
atcaatgagc attggctcat caagcatcca aaatggtggc aggacttggc taccttggag 5040
gcagagaaag ccacgacagt tgcagtattc ggtgttagag acctacaaaa tgcagtggtg 5100
ccaatttggt accacaacgg aaagatcgcg gtggcctcat caaaactgta tggcaaacaa 5160
ctgcaatttt tggggtttaa cagcaacggt gaagcttggt tgttcgactt ggacgacggc 5220
attctgtata gccagccgct tgtggatagc tttctgtatg caatctttag tttggacggg 5280
aagcttattg caccaatctt tattccacaa gtggagcaaa tgctcagcgg ccagcatctc 5340
agaaaccctg tcatcgtgga cggccgtttg catgtgacta ccatagacgg tgttgttgtt 5400
ggatttgaca agaagtcgcc aactctggtt gcagtgaaca aggcctggca gatatctcgt 5460
cagaaccgat tagaagaggc cctcggaaac ctgctgtcca attggtctga tcacaatgat 5520
gcgattgtgt tgcagggtga tccagaatca gcgccggcat ggtattatgt tccatcaaga 5580
gaaatagtga ccgccaatgg tctaacatgg gcggatcatc ctctatggct aggaatggct 5640
gtcggtgctg caacgccata catctacagc acatcccgac gccgtgtgta tgttgtggag 5700
agcaaaaaca tggccaaaga caaaggagaa ttcgcagcag cacattgctt tgcaaaatca 5760
cttgtgctac agagcatcgt tcccgaggaa ataacgattc cgacgctggc tggcatcgat 5820
ttcgcactcg ttgcagcgac aaatgcaacc attgtaatag accgagacaa ttggaagcat 5880
tacaaggcca ttgtgctcga caacaaggtg gatcaggacg gtggcgctaa ggtggtagtc 5940
ggcagtgaga ttcccatgaa agttctggtg cgcaaatttc agggaaatct ggagttcttc 6000
gatccaaaca atggtaatgt tgttatactc cagaatgtgt tgaagggaga cggagcttgt 6060
tgccagatag agatcaaagt aggcgatgct gagactatgc ttgtatccga tttctcggag 6120
atattctcaa agacgcatcc tctggtggca agccaagtat ttgagcttgg ggatgtgatt 6180
gcagtcacaa aacacaaatc ggactatgcg gatctgtggg ccattctacg catgacatag 6240
<210> 2
<211> 5985
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
<210> 3
<211> 4950
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
<210> 4
<211> 5862
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
<210> 5
<211> 6087
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
<210> 6
<211> 6240
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
atggtgatgg ttattgatta cccttctttg agggcttctt tgtatgctac taagtctgct 60
gttccttgta ggcctcttcc tggtaaccct gaaagggttt ctaggagggt ttctagaagg 120
ggtgctggtg aaattggtag tcatgctgat ttttggattt ctagagctaa ggcttctgct 180
attaggattt ttcctagaca agaaggtcca ttgagggaaa gcaggaggag gttctatttg 240
ttgagagagg ctgttttcac aggtaggagg actgcatggg ctgcaaatcc tgctgctcag 300
agagcagctt gggatagaaa gaggtctcct ttcttcggta ggtcatcaga tgatattcat 360
tcattcagta agaacccaga agagttcctt actcaaaata ctttgtcttg ttctcttctt 420
actagaactg ctcaaactgt tactaagtct cctgttttgc ctgctcaagg ttgggttaag 480
gttggtagag tgccaggtag aggtgatgct cttttcgttg agcttgttgc tgaaaaatct 540
gatgaaactg ttaaggctta cttgttgggt tataatggta agaatgattc tcctgaacct 600
gcttatgttg atattccaaa gaggggtgca gagaatggaa ctttcctttt tacagtggat 660
cttagcggtt gttctgttgt tgttactcaa ttggatgagc agacttatag agtgttccat 720
gataagaggg ttaattcaag tattctttac gataacgtgg ttatgtatgt tgattttaac 780
cattacaggt accctagagc tcatggtcat ggtagagata ctggtcaagc tgttgtttgt 840
atgcagtttg ctgatggtgt ttggaagttg ttcttgcaaa gacatgagac tgctaattct 900
cttccaaaga tttggtctag aggtgatggt aagcctgaaa ttttctctga tgctgagcct 960
agacctcata tggaggatgg tattaaggag ttccttaata gaaggagggc tttgcaagat 1020
gaattgaagc attttgctaa tactttggaa gttgatcctg atattgtttc taatgctgtt 1080
gatggtgaat atgaaggtgg tcaattttct agaaatgatg atgcaattag gggttggaat 1140
gaattgagag cttctatgag acagaagatg gctgaaagaa agaagcttct tcaaactaag 1200
aagggtatgt tggatagttt caataatcca gatcatcctc tttacaagtt cagaaaacaa 1260
gttttggatg aattgaaaga gatgtcagct tatgataata cacttgaagc ttctgaaatc 1320
gctgatagga cttggctttg gcttgaaatt aagaaggcta agggaacaaa tgcagttgtt 1380
aatagcggag ctgcacctaa gcaaccatcc cttactgttg gtgaaagata tgctgaaatg 1440
gaagcagcac tttggatgag aagcgataca gctgctttcg aacaaggata tgaaagttat 1500
caaactgtta gagttccagg ttataatgaa gatatgacaa gcattgctat gaagaagttg 1560
tttgttgaat cttccttgac agatgaacaa cttggtgctc tttgtaagag gatttccatt 1620
gctagtgatc aagaaaatca tttgttgact caaaagaccc aagaaatcga atcaactttc 1680
aagcaattct ccgatggaag cttccttgaa tctgctccac aagatttctt ccttagaaga 1740
gttggtgata aatccggtgg taggtgctac ccacttgtta gggttatggc tgttgctctt 1800
tctgaaggtg aagatgttgc tcaaaatttc atggaaaatt tgaggacttt gtcaaggaat 1860
cctgaatatt ccggtgaatc agcttcttct caagctacaa atgctggtct tttcagagat 1920
gctcttgctg gtcttcatgc aaacgttgaa gctatcgctg catccgagaa gcttcctgct 1980
gtttttggtt tgcctgatgt tgctactatg ctttctcagg cttctgaatc taaggtttat 2040
gctcttaata ctgaaactca ttctatgctt gttgctgtta ttgttgataa gcaatctttg 2100
gctaggaagt attattttta cgatcctaat ttccttatgg ctggtttctc tgatattact 2160
agattctccg aagctcttaa gtaccatttc gttaatttgc agtttgcagg taattataag 2220
gctttcggta ctgctgaagc tcctcaattc aatcttgttc ttattgatac taccgctatg 2280
gaggatgctc atgttgatgg tggtattact gtgaagcaac tttctgaagg agttgatctt 2340
actaatgttt tcgattctac taagaaggct agagtggtta ctgaggctgc agctaggttg 2400
gctgaagatc agcaattgag aactggactt acaattattg aagctgatca gtgggctaat 2460
agatggcagg atgctgttat taggttgaag cttaaggaag gtctttctgg tgagtggatg 2520
cctttgcttg aaaagatgca agaaaatgca gatggtagtt atcagattga tttcattaac 2580
actaagaaca ctggagaaat taggggagtt tcaactaggg atgctacttt taaggaattc 2640
aagtcttatt tggataagaa ccttgatgtg ttgcataagg attttatttt ccaggatgga 2700
gtgttcaaac ctcttgaggg tgagcctatt gatggactta atgctatgtt cgctgttcaa 2760
atgattattg attggttcaa taggggtact aacgcagaag aagatgctgc ttctggttct 2820
actaacgctc aattgtcttt ggctattaag gttcattctt atatcaacat ggcacaagtg 2880
gcacatactg ctgttactga tgtggcaaag gtttataatt tggtgaaatc tgctctttac 2940
ggtgaacaag ttgctggtga aacatcattg ttcactttcg gtaaggctct taaatttgct 3000
gctactgaag gtcttggtgt gttgcttggt gcttcttttg tgatccttga tgcttatcag 3060
ctttctcttg ttgagaatga tgttcagaag gcaattattg gtactcaact tgcttttgat 3120
tctgcttctt ttcttgctgg tattggtgct attgttgctg gtgcttctgg tgttggtatg 3180
attttgggtg gtgctggtgt tgttttgggt ggtttgggtg ttggttttgg tgctttggct 3240
caggcttttg gtgagattac tgctgatgct cagttggttg gtaagtattt tgctttgttg 3300
gatgaggctt ataggggtgg tggttatagg tatgagtcta aggataaggc tttgatgcct 3360
ttggctggtg ctgtgattga taagattaga cttgctgata agcatttgca agttcatttt 3420
gattctcaat tcatctacag gactcatcat ggttctactg gttctggtaa gattaattat 3480
ttcttctggg ctggtgatat gcctcaaatg gttcatgata gatctcaagc tattaatgtt 3540
agggagcaga ttggttataa gaatgataat gctactcttg aacttccaga ggatgcttct 3600
gatcttattc ttcctgcaac tcctaagtct ttcattacat atagatacga gatcttgcca 3660
ttcgctactt ctagacatga tactggtttt gatgttatta gaagacttga ggaggattat 3720
agattcgatt atgatttcta catcttccct tctgaatata ccattagaac tattaagcac 3780
gaatacgttg aaaccaaggt tgctgttgtg cttgatagaa gatctttgag acttggtgct 3840
cctgagcttt tgaaggaaca acatggttat cttatttacg agattgaagg tacaggtggt 3900
caatatgtta tttgtttgaa tcagggttct actgtgactc ttaagacaac tggttctgaa 3960
gcaaataaga ctctttggat tttggatgct aggcaactta ttgctggtaa tgatagtatc 4020
actgtttctc aagattctat tactatcggt tctactactg tgcatcttag gaatcaggat 4080
ttcgattgtc ttcagattgt tatgtctaga gaagaacttc ttcaagttga tttggcttct 4140
ggtgaagcta gagttttctc cgaagatgct tctaagtggc ttgatacaaa ggaaactatt 4200
catgaacacc ttcacaagtt ggctgaagaa cataggcttc atggtcaatt cgttgttgtt 4260
aataattact acaggggtgt tggtagagct tattatgatg ctgctcatga tagaatgctt 4320
tatactgatg ttgaggatga aactcttaca agagatgctg ttcttgctgg tgttactggt 4380
gaagatgctt atttttataa cgttaagcag aacgctttgt ggagagttaa cgctggtact 4440
caccaagtta ttactaagta ttatcctact cttcttgagg atcctgattc taacatggct 4500
agagttgagc aacatggtaa tatcgttgtt atggctatgt caagattgaa tcatcctgat 4560
aagggtgtta acggtattat ttatcttgtt catcaggaat ctatcgttct tactgctgtt 4620
attggaaact ctgaaatgct taagagactt agacaaactt ctactatctc tcttactgaa 4680
cttaggggtg ttaacgatat ggctcttgtt tcaactatgg ataggcttcc tggtcagaag 4740
gtggtggcta caaatgcaga attcgttaca cttttggcta aggatgaaca aggagctaat 4800
attaaggaac aaactgttta ttgccagcaa ggtactggtc aaactaatga tacttctatt 4860
gaatcaatcc ctatccctgg tttgtctaat cttcttacag tttatggtga ggttttcgct 4920
acaactaatg atggtagaat ccttagattg cttggtgatg gaagcacttg tcttgagggt 4980
atcaatgaac attggttgat taagcatcct aaatggtggc aggatcttgc tactcttgag 5040
gcagagaagg ctaccactgt tgctgttttt ggagttaggg atttgcaaaa tgctgttgtt 5100
cctatttggt atcataacgg taagattgca gttgcttcat ctaaacttta tggtaagcaa 5160
ttgcagtttt tgggttttaa ttctaacgga gaagcttggc ttttcgatct tgatgatggt 5220
attttgtatt ctcaaccttt ggttgattct ttcctttatg ctattttcag tttggatggt 5280
aagttgattg ctcctatctt tattcctcag gttgaacaaa tgttgtccgg tcaacatctt 5340
aggaaccctg ttattgttga tggtaggctt catgttacta ctattgatgg tgttgttgtt 5400
ggtttcgata agaagtctcc tactcttgtg gctgttaata aggcttggca aatttctaga 5460
caaaataggc ttgaggaggc tttgggtaat ttgttgtcta attggtctga tcataacgat 5520
gctattgttc ttcagggtga tcctgagtct gctcctgctt ggtattatgt tccttctagg 5580
gagattgtga ctgctaatgg tttgacttgg gctgatcacc ctctttggct tggaatggct 5640
gttggtgctg ctactcctta tatttattct acttctagaa ggagggttta tgttgttgag 5700
tctaagaata tggctaagga taagggtgaa tttgctgctg ctcattgttt tgctaagtct 5760
ttggttcttc agtctattgt tcctgaggaa attactattc caactcttgc tggtatcgat 5820
ttcgctttgg ttgctgctac taatgctact attgttattg atagggataa ctggaagcat 5880
tataaggcaa ttgttcttga taacaaggtt gatcaagatg gaggtgctaa ggttgtggtt 5940
ggttctgaga ttcctatgaa ggttcttgtg aggaagtttc aaggtaattt ggaatttttc 6000
gatcctaata acggtaatgt ggttattctt caaaacgttc ttaagggaga tggtgcttgt 6060
tgtcagattg agattaaggt tggtgatgca gagactatgt tggtttctga tttctctgag 6120
attttctcta agactcatcc tttggttgct tctcaggttt tcgaacttgg tgatgttatt 6180
gctgttacta agcataagag tgattatgct gatctttggg ctattttgag gatgacttga 6240
<210> 7
<211> 5985
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
<210> 8
<211> 4590
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
<210> 9
<211> 5862
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
<210> 10
<211> 6087
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
<210> 11
<211> 2079
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 11
Met Val Met Val Ile Asp Tyr Pro Ser Leu Arg Ala Ser Leu Tyr Ala
1 5 10 15
Thr Lys Ser Ala Val Pro Cys Arg Pro Leu Pro Gly Asn Pro Glu Arg
20 25 30
Val Ser Arg Arg Val Ser Arg Arg Gly Ala Gly Glu Ile Gly Ser His
35 40 45
Ala Asp Phe Trp Ile Ser Arg Ala Lys Ala Ser Ala Ile Arg Ile Phe
50 55 60
Pro Arg Gln Glu Gly Pro Leu Arg Glu Ser Arg Arg Arg Phe Tyr Leu
65 70 75 80
Leu Arg Glu Ala Val Phe Thr Gly Arg Arg Thr Ala Trp Ala Ala Asn
85 90 95
Pro Ala Ala Gln Arg Ala Ala Trp Asp Arg Lys Arg Ser Pro Phe Phe
100 105 110
Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu Glu
115 120 125
Phe Leu Thr Gln Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr Ala
130 135 140
Gln Thr Val Thr Lys Ser Pro Val Leu Pro Ala Gln Gly Trp Val Lys
145 150 155 160
Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu Phe Val Glu Leu Val
165 170 175
Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr Leu Leu Gly Tyr Asn
180 185 190
Gly Lys Asn Asp Ser Pro Glu Pro Ala Tyr Val Asp Ile Pro Lys Arg
195 200 205
Gly Ala Glu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly Cys
210 215 220
Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His
225 230 235 240
Asp Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met Tyr
245 250 255
Val Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly Arg
260 265 270
Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val Trp
275 280 285
Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile
290 295 300
Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro
305 310 315 320
Arg Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg
325 330 335
Ala Leu Gln Asp Glu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp
340 345 350
Pro Asp Ile Val Ser Asn Ala Val Asp Gly Glu Tyr Glu Gly Gly Gln
355 360 365
Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg Ala
370 375 380
Ser Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu Leu Gln Thr Lys
385 390 395 400
Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr Lys
405 410 415
Phe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp
420 425 430
Asn Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu
435 440 445
Glu Ile Lys Lys Ala Lys Gly Thr Asn Ala Val Val Asn Ser Gly Ala
450 455 460
Ala Pro Lys Gln Pro Ser Leu Thr Val Gly Glu Arg Tyr Ala Glu Met
465 470 475 480
Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu Gln Gly
485 490 495
Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu Asp Met
500 505 510
Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp
515 520 525
Glu Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln
530 535 540
Glu Asn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe
545 550 555 560
Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp Phe
565 570 575
Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro Leu
580 585 590
Val Arg Val Met Ala Val Ala Leu Ser Glu Gly Glu Asp Val Ala Gln
595 600 605
Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser
610 615 620
Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp
625 630 635 640
Ala Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu
645 650 655
Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser
660 665 670
Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His Ser
675 680 685
Met Leu Val Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr
690 695 700
Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr
705 710 715 720
Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala
725 730 735
Gly Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu
740 745 750
Val Leu Ile Asp Thr Thr Ala Met Glu Asp Ala His Val Asp Gly Gly
755 760 765
Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val Phe
770 775 780
Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg Leu
785 790 795 800
Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp
805 810 815
Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys
820 825 830
Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu
835 840 845
Asn Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr
850 855 860
Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe
865 870 875 880
Lys Ser Tyr Leu Asp Lys Asn Leu Asp Val Leu His Lys Asp Phe Ile
885 890 895
Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly
900 905 910
Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg
915 920 925
Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln
930 935 940
Leu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val
945 950 955 960
Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys
965 970 975
Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Glu Thr Ser Leu Phe Thr
980 985 990
Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu
995 1000 1005
Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val
1010 1015 1020
Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp
1025 1030 1035 1040
Ser Ala Ser Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser
1045 1050 1055
Gly Val Gly Met Ile Leu Gly Gly Ala Gly Val Val Leu Gly Gly Leu
1060 1065 1070
Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Glu Ile Thr Ala
1075 1080 1085
Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala Tyr
1090 1095 1100
Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro
1105 1110 1115 1120
Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu
1125 1130 1135
Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Ser
1140 1145 1150
Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro
1155 1160 1165
Gln Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln Ile
1170 1175 1180
Gly Tyr Lys Asn Asp Asn Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser
1185 1190 1195 1200
Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr
1205 1210 1215
Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Thr Gly Phe Asp Val
1220 1225 1230
Ile Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile
1235 1240 1245
Phe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu
1250 1255 1260
Thr Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala
1265 1270 1275 1280
Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile Glu
1285 1290 1295
Gly Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn Gln Gly Ser Thr Val
1300 1305 1310
Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu
1315 1320 1325
Asp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln
1330 1335 1340
Asp Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp
1345 1350 1355 1360
Phe Asp Cys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln Val
1365 1370 1375
Asp Leu Ala Ser Gly Glu Ala Arg Val Phe Ser Glu Asp Ala Ser Lys
1380 1385 1390
Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala
1395 1400 1405
Glu Glu His Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr
1410 1415 1420
Arg Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu
1425 1430 1435 1440
Tyr Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala
1445 1450 1455
Gly Val Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala
1460 1465 1470
Leu Trp Arg Val Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr
1475 1480 1485
Pro Thr Leu Leu Glu Asp Pro Asp Ser Asn Met Ala Arg Val Glu Gln
1490 1495 1500
His Gly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp
1505 1510 1515 1520
Lys Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Ile Val
1525 1530 1535
Leu Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln
1540 1545 1550
Thr Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala
1555 1560 1565
Leu Val Ser Thr Met Asp Arg Leu Pro Gly Gln Lys Val Val Ala Thr
1570 1575 1580
Asn Ala Glu Phe Val Thr Leu Leu Ala Lys Asp Glu Gln Gly Ala Asn
1585 1590 1595 1600
Ile Lys Glu Gln Thr Val Tyr Cys Gln Gln Gly Thr Gly Gln Thr Asn
1605 1610 1615
Asp Thr Ser Ile Glu Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu Leu
1620 1625 1630
Thr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly Arg Ile Leu
1635 1640 1645
Arg Leu Leu Gly Asp Gly Ser Thr Cys Leu Glu Gly Ile Asn Glu His
1650 1655 1660
Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala Thr Leu Glu
1665 1670 1675 1680
Ala Glu Lys Ala Thr Thr Val Ala Val Phe Gly Val Arg Asp Leu Gln
1685 1690 1695
Asn Ala Val Val Pro Ile Trp Tyr His Asn Gly Lys Ile Ala Val Ala
1700 1705 1710
Ser Ser Lys Leu Tyr Gly Lys Gln Leu Gln Phe Leu Gly Phe Asn Ser
1715 1720 1725
Asn Gly Glu Ala Trp Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser
1730 1735 1740
Gln Pro Leu Val Asp Ser Phe Leu Tyr Ala Ile Phe Ser Leu Asp Gly
1745 1750 1755 1760
Lys Leu Ile Ala Pro Ile Phe Ile Pro Gln Val Glu Gln Met Leu Ser
1765 1770 1775
Gly Gln His Leu Arg Asn Pro Val Ile Val Asp Gly Arg Leu His Val
1780 1785 1790
Thr Thr Ile Asp Gly Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr
1795 1800 1805
Leu Val Ala Val Asn Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu
1810 1815 1820
Glu Glu Ala Leu Gly Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp
1825 1830 1835 1840
Ala Ile Val Leu Gln Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr
1845 1850 1855
Val Pro Ser Arg Glu Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp
1860 1865 1870
His Pro Leu Trp Leu Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile
1875 1880 1885
Tyr Ser Thr Ser Arg Arg Arg Val Tyr Val Val Glu Ser Lys Asn Met
1890 1895 1900
Ala Lys Asp Lys Gly Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser
1905 1910 1915 1920
Leu Val Leu Gln Ser Ile Val Pro Glu Glu Ile Thr Ile Pro Thr Leu
1925 1930 1935
Ala Gly Ile Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val
1940 1945 1950
Ile Asp Arg Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn
1955 1960 1965
Lys Val Asp Gln Asp Gly Gly Ala Lys Val Val Val Gly Ser Glu Ile
1970 1975 1980
Pro Met Lys Val Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe
1985 1990 1995 2000
Asp Pro Asn Asn Gly Asn Val Val Ile Leu Gln Asn Val Leu Lys Gly
2005 2010 2015
Asp Gly Ala Cys Cys Gln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr
2020 2025 2030
Met Leu Val Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu
2035 2040 2045
Val Ala Ser Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys
2050 2055 2060
His Lys Ser Asp Tyr Ala Asp Leu Trp Ala Ile Leu Arg Met Thr
2065 2070 2075
<210> 12
<211> 1994
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 12
Met Glu Glu Ala Asp Met Glu Asn Arg Gly Tyr Glu Glu Arg His Ser
1 5 10 15
Gln Ser Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Phe
20 25 30
Phe Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu
35 40 45
Glu Phe Leu Thr Lys Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr
50 55 60
Ala Gln Thr Val Thr Lys Ser Pro Val Leu Pro Ala Gln Gly Trp Val
65 70 75 80
Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu Ser Gly Cys Ser
85 90 95
Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe His Asp
100 105 110
Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met Tyr Val
115 120 125
Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly Arg Asp
130 135 140
Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val Trp Lys
145 150 155 160
Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys Ile Trp
165 170 175
Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu Pro Arg
180 185 190
Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg Arg Ala
195 200 205
Leu Gln Asp Glu Leu Lys His Phe Ala Asn Thr Leu Glu Val Asp Pro
210 215 220
Glu Ile Val Ser Asn Ala Val Asp Gly Glu Tyr Glu Gly Gly Gln Phe
225 230 235 240
Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg Ala Ser
245 250 255
Met Arg Gln Lys Met Ala Glu Arg Lys Lys Arg Leu Glu Arg Asn Lys
260 265 270
Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr Lys Phe
275 280 285
Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr Asp Asn
290 295 300
Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp Leu Glu
305 310 315 320
Ile Lys Lys Ala Lys Gly Thr Asn Ala Val Val Asn Ser Gly Ala Ala
325 330 335
Pro Lys Gln Pro Ser Leu Thr Val Gly Glu Arg Tyr Ala Glu Met Glu
340 345 350
Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu Gln Gly Tyr
355 360 365
Glu Ser Tyr Gln Thr Leu Arg Val Pro Gly Tyr Asn Glu Asp Met Thr
370 375 380
Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr Asp Glu
385 390 395 400
Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp Gln Glu
405 410 415
Asn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr Phe Lys
420 425 430
Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp Phe Phe
435 440 445
Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro Leu Val
450 455 460
Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn
465 470 475 480
Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly
485 490 495
Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala
500 505 510
Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys
515 520 525
Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser Gln
530 535 540
Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His Ser Met
545 550 555 560
Leu Val Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr Tyr
565 570 575
Phe Tyr Asp Pro Asn Phe Leu Ile Ala Gly Phe Ser Asp Ile Thr Arg
580 585 590
Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly
595 600 605
Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val
610 615 620
Leu Ile Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly Gly Ile
625 630 635 640
Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val Phe Asp
645 650 655
Ser Thr Lys Lys Ala Arg Val Val Thr Glu Thr Ala Ala Arg Leu Ala
660 665 670
Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln
675 680 685
Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu
690 695 700
Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln Glu Asn
705 710 715 720
Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn Thr Gly
725 730 735
Glu Ile Arg Gly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe Lys
740 745 750
Ser Tyr Leu Asp Lys Asn Leu Asp Val Leu His Lys Asp Phe Ile Phe
755 760 765
Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp Gly Leu
770 775 780
Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Arg Gly
785 790 795 800
Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu
805 810 815
Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala
820 825 830
His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys Ser
835 840 845
Ala Leu Tyr Gly Glu Gln Val Ala Gly Glu Ala Ser Leu Leu Thr Phe
850 855 860
Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu Leu
865 870 875 880
Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu
885 890 895
Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser
900 905 910
Ala Ser Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly
915 920 925
Val Gly Met Ile Leu Gly Gly Ala Gly Val Val Leu Gly Gly Leu Gly
930 935 940
Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Glu Ile Thr Ala Asp
945 950 955 960
Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala Tyr Arg
965 970 975
Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu
980 985 990
Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His Leu Gln
995 1000 1005
Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Leu Thr
1010 1015 1020
Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met Pro Gln
1025 1030 1035 1040
Met Val His Asp Arg Ser Gln Ala Ile Asn Ile Arg Glu Gln Ile Gly
1045 1050 1055
Tyr Lys Asn Asp His Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser Asp
1060 1065 1070
Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Gln Tyr Gln
1075 1080 1085
Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile
1090 1095 1100
Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe
1105 1110 1115 1120
Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr
1125 1130 1135
Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala Pro
1140 1145 1150
Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile Glu Gly
1155 1160 1165
Thr Gly Gly Gln Tyr Val Ile Cys Leu Asn Gln Gly Ser Thr Val Thr
1170 1175 1180
Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp
1185 1190 1195 1200
Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp
1205 1210 1215
Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe
1220 1225 1230
Asp Cys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln Val Asp
1235 1240 1245
Leu Ala Ser Gly Glu Ala Arg Val Phe Ser Glu Asp Ala Ser Lys Trp
1250 1255 1260
Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala Glu
1265 1270 1275 1280
Glu His Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr Arg
1285 1290 1295
Gly Val Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr
1300 1305 1310
Thr Asp Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly
1315 1320 1325
Val Thr Gly Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu
1330 1335 1340
Trp Arg Val Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro
1345 1350 1355 1360
Thr Leu Leu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His
1365 1370 1375
Gly Asn Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp Lys
1380 1385 1390
Gly Val Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu
1395 1400 1405
Thr Ala Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr
1410 1415 1420
Ser Thr Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu
1425 1430 1435 1440
Val Ser Thr Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn
1445 1450 1455
Ala Glu Phe Val Thr Ile Leu Ala Lys Asp Glu Gln Gly Ser Gln Val
1460 1465 1470
Cys Phe Trp Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile
1475 1480 1485
Gln Pro Pro Ile Pro Thr Asp Leu Thr Leu Thr Val Ser Ser His Lys
1490 1495 1500
Val Ala Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys Glu Gln Thr
1505 1510 1515 1520
Val Tyr Cys Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Ile Glu
1525 1530 1535
Ser Ile Pro Ile Pro Gly Leu Ser Asn Leu Leu Thr Val Tyr Gly Glu
1540 1545 1550
Val Phe Ala Thr Thr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp
1555 1560 1565
Gly Ser Thr Tyr Leu Asp Gly Ile Asn Glu His Trp Leu Ile Lys His
1570 1575 1580
Pro Lys Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr
1585 1590 1595 1600
Thr Val Ala Val Phe Gly Val Arg Asp Leu Gln Asn Ala Val Val Pro
1605 1610 1615
Ile Trp Tyr His Asn Gly Lys Ile Ala Val Ala Ser Ser Lys Leu Tyr
1620 1625 1630
Gly Lys Gln Leu Gln Phe Leu Gly Phe Asn Ser Asn Gly Glu Ala Trp
1635 1640 1645
Leu Phe Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp
1650 1655 1660
Ser Phe Leu Tyr Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro
1665 1670 1675 1680
Ile Phe Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg
1685 1690 1695
Asn Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly
1700 1705 1710
Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Val Asn
1715 1720 1725
Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly
1730 1735 1740
Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln
1745 1750 1755 1760
Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu
1765 1770 1775
Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu
1780 1785 1790
Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg
1795 1800 1805
Arg Arg Val Tyr Ala Val Glu Ser Lys His Met Ala Lys Asp Lys Gly
1810 1815 1820
Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser
1825 1830 1835 1840
Ile Val Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Ile Asp Phe
1845 1850 1855
Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile His Arg Asp Asn
1860 1865 1870
Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp
1875 1880 1885
Gly Gly Ala Lys Val Val Val Gly Ser Gly Ile Pro Met Lys Val Leu
1890 1895 1900
Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly
1905 1910 1915 1920
Asn Val Val Ile Leu Arg Asn Val Leu Lys Gly Asp Gly Ala Cys Ser
1925 1930 1935
Gln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp
1940 1945 1950
Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val
1955 1960 1965
Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser Asp Tyr
1970 1975 1980
Ala Asp Leu Trp Ala Ile Leu Arg Met Thr
1985 1990
<210> 13
<211> 1529
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 13
Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala Gln Asn Phe Met
1 5 10 15
Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr Ser Gly Glu Ser
20 25 30
Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg Asp Ala Leu Ala
35 40 45
Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser Glu Lys Leu Pro
50 55 60
Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu Ser Gln Ala Ser
65 70 75 80
Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His Ser Met Leu Ala
85 90 95
Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys Tyr Tyr Phe Tyr
100 105 110
Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile Thr Arg Phe Ser
115 120 125
Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe Ala Gly Asn Tyr
130 135 140
Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn Leu Val Leu Ile
145 150 155 160
Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly Gly Ile Thr Val
165 170 175
Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val Phe Asp Ser Thr
180 185 190
Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg Leu Ala Glu Asp
195 200 205
Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala Asp Gln Trp Ala
210 215 220
Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu Lys Glu Gly Leu
225 230 235 240
Ser Gly Glu Trp Met Pro Leu Leu Glu Arg Met Gln Glu Asn Gly His
245 250 255
Gly Ser Tyr Gln Ile Glu Phe Leu Asn Thr Lys Asn Thr Gly Glu Thr
260 265 270
Arg Trp Val Ser Thr Arg Asp Ala Thr Phe Lys Glu Phe Lys Ser Tyr
275 280 285
Leu Asp Lys Asn Phe Asp Ala Leu Arg Lys Asp Phe Ile Phe Gln Asp
290 295 300
Gly Val Phe Lys Pro Leu Asp Gly Glu Pro Ile Asp Gly Leu Asn Ala
305 310 315 320
Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn Pro Gly Thr Asn
325 330 335
Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala Gln Leu Ser Leu
340 345 350
Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln Val Ala His Thr
355 360 365
Ala Val Thr Asp Val Ala Lys Val Tyr Asn Leu Val Lys Ser Ala Leu
370 375 380
Tyr Gly Glu Gln Val Ala Gly Glu Ala Ser Leu Leu Thr Phe Gly Lys
385 390 395 400
Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val Leu Leu Gly Ala
405 410 415
Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu Val Glu Asn Asp
420 425 430
Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe Asp Ser Ala Ser
435 440 445
Phe Leu Ala Gly Ile Gly Ala Ile Val Ala Gly Ala Ser Gly Val Gly
450 455 460
Met Ile Leu Gly Gly Ala Gly Val Ile Leu Gly Gly Leu Gly Val Gly
465 470 475 480
Phe Gly Ala Leu Ala Gln Ala Phe Gly Gln Ile Thr Ala Asp Ala Gln
485 490 495
Leu Val Gly Glu Tyr Phe Ala Leu Leu Asp Glu Ala Tyr Ser Gly Gly
500 505 510
Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met Pro Leu Ala Gly
515 520 525
Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys Arg Leu Gln Val His
530 535 540
Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly Ser Thr Gly Ser
545 550 555 560
Gly Lys Ile Asn Tyr Phe Phe Trp Val Gly Asp Met Pro Gln Met Val
565 570 575
His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln Ile Gly Tyr Lys
580 585 590
Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala Ser Asp Leu Ile
595 600 605
Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg Tyr Glu Ile Leu
610 615 620
Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp Val Ile Arg Arg
625 630 635 640
Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr Ile Phe Pro Ser
645 650 655
Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val Glu Thr Lys Val
660 665 670
Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly Ala Pro Glu Leu
675 680 685
Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile Glu Gly Thr Gly
690 695 700
Gly Gln Tyr Val Ile Cys Leu Asn His Gly Ser Thr Val Thr Leu Lys
705 710 715 720
Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile Leu Asp Ala Arg
725 730 735
Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser Gln Asp Ser Ile
740 745 750
Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln Asp Phe Asp Cys
755 760 765
Leu Gln Ile Val Met Ser Arg Asp Glu Leu Leu Gln Val Asp Leu Ala
770 775 780
Ser Arg Glu Val Arg Val Phe Ser Glu Asp Ala Ser Lys Trp Leu Asp
785 790 795 800
Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu Ala Gly Glu His
805 810 815
Arg Leu His Gly Gln Phe Val Val Val Asn Asn Tyr Tyr Arg Gly Val
820 825 830
Gly Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr Thr Asp
835 840 845
Val Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val Thr
850 855 860
Gly Glu Asp Ala Tyr Phe Tyr Asn Val Asn Gln Asn Ala Leu Trp Arg
865 870 875 880
Val Asn Ala Asp Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Leu
885 890 895
Leu Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn
900 905 910
Ile Val Val Met Ala Met Ser Arg Leu Asn His Pro Asp Lys Gly Val
915 920 925
Asn Gly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu Thr Ala
930 935 940
Val Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr
945 950 955 960
Ile Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser
965 970 975
Thr Met Asp Arg Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu
980 985 990
Phe Val Thr Leu Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe
995 1000 1005
Trp Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln Pro
1010 1015 1020
Pro Ile Pro Thr Asp Leu Thr Leu Thr Val Ser Ser His Lys Val Ala
1025 1030 1035 1040
Gly Gln Asp Val Phe Tyr Phe Tyr Ser Ile Lys Glu Gln Thr Val Tyr
1045 1050 1055
Cys Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Ile Glu Ser Ile
1060 1065 1070
Pro Ile Pro Gly Leu Ser Asn Leu Leu Thr Val Tyr Gly Glu Val Phe
1075 1080 1085
Ala Thr Thr Asn Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser
1090 1095 1100
Thr Tyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His Pro Lys
1105 1110 1115 1120
Trp Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Lys Ala Thr Thr Val
1125 1130 1135
Ala Val Phe Gly Val Arg Asp Pro Gln Asn Ala Val Val Pro Ile Trp
1140 1145 1150
Tyr His Asn Gly Lys Ile Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys
1155 1160 1165
Gln Leu Gln Phe Leu Gly Phe Asn Ser Asn Gly Glu Ala Trp Leu Phe
1170 1175 1180
Asp Leu Asp Asp Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Ser Gln
1185 1190 1195 1200
Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro Asn
1205 1210 1215
Leu Ile Pro Gln Val Glu Gln Met Leu Ser Gly Gln His Leu Arg Asn
1220 1225 1230
Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val
1235 1240 1245
Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Val Asn Lys
1250 1255 1260
Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly Asn
1265 1270 1275 1280
Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln Gly
1285 1290 1295
Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile
1300 1305 1310
Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly
1315 1320 1325
Met Ala Val Gly Ala Ala Thr Leu Tyr Ile Tyr Ser Thr Ser Arg Arg
1330 1335 1340
Arg Val Tyr Ala Val Glu Ser Lys Lys Met Ala Lys Asp Lys Gly Glu
1345 1350 1355 1360
Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser Ile
1365 1370 1375
Val Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Thr Asp Phe Ala
1380 1385 1390
Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile Asp Arg Asp Asn Trp
1395 1400 1405
Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp Gly
1410 1415 1420
Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu Val
1425 1430 1435 1440
Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly Asn
1445 1450 1455
Val Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Ser Lys
1460 1465 1470
Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp Phe
1475 1480 1485
Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val Phe
1490 1495 1500
Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser Asp Tyr Ala
1505 1510 1515 1520
Asp Leu Trp Ala Ile Leu Arg Met Thr
1525
<210> 14
<211> 1953
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 14
Met Glu Glu Val Asp Met Gln Asn Arg Gly Tyr Glu Glu Trp His Ser
1 5 10 15
Gln Ser Gln Ser Ala Thr Thr Leu Arg Arg Arg Lys Arg Ser Pro Val
20 25 30
Phe Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu
35 40 45
Glu Phe Leu Thr Gln Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr
50 55 60
Ala Glu Thr Val Thr Lys Ser Pro Val Leu Pro Glu Arg Gly Trp Val
65 70 75 80
Lys Val Gly Arg Val Pro Gly Arg Gly Asp Ala Leu Phe Val Gln Val
85 90 95
Val Ala Glu Gln Ser Tyr Asp Thr Val Lys Ala Tyr Leu Leu Gly Tyr
100 105 110
Asn Gly Glu Asn Asp Ser Pro Asn Pro Ala Tyr Val Asp Ile Pro Lys
115 120 125
Arg Ala Ala Glu Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly
130 135 140
Cys Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe
145 150 155 160
His Asp Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met
165 170 175
Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly
180 185 190
Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val
195 200 205
Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys
210 215 220
Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Glu
225 230 235 240
Pro Arg Gln His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg
245 250 255
Arg Ala Leu Gln Asp Glu Leu Lys Lys Phe Ala Asp Met Phe Gly Val
260 265 270
Asp Pro Glu Ile Val Ser Asn Ala Val Asp Gly Lys Tyr Lys Gly Gly
275 280 285
Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg
290 295 300
Ala Ser Met Arg Gln Lys Met Ala Glu Arg Asn Lys Val Leu Leu Thr
305 310 315 320
Met Lys Leu Glu Gln Pro Arg Ser Ser Arg Val Leu Pro Leu Val Asn
325 330 335
Ala Thr Gln Arg Trp Arg Arg Pro Phe Gly Cys Val Ala Thr Gln Arg
340 345 350
Arg Leu Ser Lys Ala Gly Arg Leu Val Gln Ala Tyr Ile His Arg Asp
355 360 365
Gln Glu Asn His Leu Leu Thr Gln Lys Thr Gln Glu Ile Glu Ser Thr
370 375 380
Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp
385 390 395 400
Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro
405 410 415
Leu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala
420 425 430
Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Lys Asn Pro Glu Tyr
435 440 445
Ser Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg
450 455 460
Asp Ala Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser
465 470 475 480
Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu
485 490 495
Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His
500 505 510
Ser Met Leu Ala Ala Val Ile Val Asp Lys Gln Ser Leu Ala Arg Lys
515 520 525
Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile
530 535 540
Thr Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe
545 550 555 560
Ala Gly Asn Tyr Lys Pro Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn
565 570 575
Leu Val Leu Ile Asp Thr Thr Val Met Ala Asp Ala His Val Asp Gly
580 585 590
Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val
595 600 605
Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg
610 615 620
Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala
625 630 635 640
Asp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Lys Leu
645 650 655
Lys Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln
660 665 670
Glu Asn Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn
675 680 685
Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Gly Thr Phe Lys Glu
690 695 700
Leu Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys Asp Phe
705 710 715 720
Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp
725 730 735
Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn
740 745 750
Arg Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala
755 760 765
Gln Leu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln
770 775 780
Val Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Ser Leu Val
785 790 795 800
Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala Ser Leu Leu
805 810 815
Thr Phe Gly Lys Ala Leu Lys Leu Ala Ala Thr Glu Gly Leu Gly Val
820 825 830
Leu Leu Gly Ala Pro Val Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu
835 840 845
Val Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Asp Phe
850 855 860
Asp Ser Ala Ser Phe Leu Val Ser Ile Gly Ala Ala Val Ala Gly Val
865 870 875 880
Ser Gly Val Gly Ile Val Leu Ser Gly Ala Gly Val Leu Leu Ala Gly
885 890 895
Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Glu Ile Thr
900 905 910
Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala
915 920 925
Tyr Arg Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met
930 935 940
Pro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His
945 950 955 960
Leu Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly
965 970 975
Ser Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Val Gly Asp Met
980 985 990
Pro Lys Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln
995 1000 1005
Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala
1010 1015 1020
Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg
1025 1030 1035 1040
Tyr Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp
1045 1050 1055
Val Ile Arg Arg Leu Glu Glu Asp His Arg Phe Asp Tyr Val Phe Tyr
1060 1065 1070
Ile Phe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val
1075 1080 1085
Glu Thr Lys Val Ala Val Val Pro Asp Arg Arg Pro Leu Arg Leu Arg
1090 1095 1100
Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile
1105 1110 1115 1120
Glu Gly Thr Gly Gly Glu Tyr Val Ile Cys Leu Asn Lys Gly Ser Thr
1125 1130 1135
Val Thr Leu Phe Thr Thr Gly Ser Glu Ala Asn Lys Thr Ser Trp Ile
1140 1145 1150
Leu Asp Ala Arg Gln Ile Ile Ala Gly Asn Asp Pro Ile Ile Val Ser
1155 1160 1165
Gln Asp Ser Ile Thr Met Gly Ser Thr Thr Val His Leu Arg Asn His
1170 1175 1180
Asp Phe Asp Cys Leu Arg Ile Val Thr Ser Arg Glu Glu Leu Leu Gln
1185 1190 1195 1200
Val Asp Ser Ala Ser Arg Gln Thr Arg Val Leu Ser Glu Asp Ala Ser
1205 1210 1215
Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu
1220 1225 1230
Ala Glu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr
1235 1240 1245
Ser Val Gln Glu Gln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly
1250 1255 1260
Arg Ala His Asp Arg Met Leu Tyr Thr Asp Val Glu Asp Glu Thr Leu
1265 1270 1275 1280
Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly Glu Asp Ala Tyr Phe
1285 1290 1295
Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val Asn Ala Gly Thr His
1300 1305 1310
Gln Val Ile Thr Lys Tyr Tyr Pro Thr Val Leu Glu Asp Pro Asp Ser
1315 1320 1325
Lys Met Ala Arg Val Glu Gln Arg Gly Asn Ile Val Val Met Ala Met
1330 1335 1340
Ser Arg Leu Asn His Ser Asp Lys Gly Val Asn Gly Ile Ile Tyr Leu
1345 1350 1355 1360
Val His Gln Glu Ser Met Val Leu Thr Ala Val Ile Gly Asn Ser Glu
1365 1370 1375
Met Leu Lys Arg Leu Arg Gln Ala Ser Thr Ile Ser Leu Thr Glu Leu
1380 1385 1390
Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr Met Asp Lys Leu Pro
1395 1400 1405
Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe Val Thr Ile Leu Ala
1410 1415 1420
Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp Leu Arg Thr Val Asp
1425 1430 1435 1440
Gly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro Ile Pro Thr Asp Leu
1445 1450 1455
Thr Leu Thr Val Ser Ser His Lys Val Ala Gly Gln Asp Val Phe Tyr
1460 1465 1470
Phe Tyr Ser Ile Lys Glu Gln Arg Val Tyr Arg Gln Gln Gly Thr Gly
1475 1480 1485
Gln Thr Asn Asp Thr Ser Val Gly Ser Ile Pro Ile Pro Gly Leu Ser
1490 1495 1500
Asn Leu Phe Thr Val Tyr Gly Glu Val Phe Ala Thr Thr Asn Asp Gly
1505 1510 1515 1520
Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr Tyr Leu Glu Gly Ile
1525 1530 1535
Asn Glu His Trp Leu Ile Lys His Pro Lys Trp Trp Gln Asp Leu Ala
1540 1545 1550
Thr Leu Glu Ala Glu Lys Ala Thr Thr Ile Ala Val Phe Gly Val Arg
1555 1560 1565
Asp Pro Gln Asn Ala Val Val Pro Ile Cys Asn Gly Glu Ala Trp Leu
1570 1575 1580
Phe Asp Leu Asp Gly Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Ser
1585 1590 1595 1600
Gln Ala Val Asp Ala Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro
1605 1610 1615
Asn Leu Ile Pro Gln Val Gln Gln Met Leu Ser Gly Gln His Leu Arg
1620 1625 1630
Asn Pro Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly
1635 1640 1645
Val Val Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Gly Asn
1650 1655 1660
Lys Ala Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Arg
1665 1670 1675 1680
Asn Leu Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln
1685 1690 1695
Gly Asp Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu
1700 1705 1710
Ile Val Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu
1715 1720 1725
Gly Met Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Ser Thr Ser Arg
1730 1735 1740
Cys Arg Val Tyr Ala Val Glu Ser Lys Asn Met Ala Lys Asp Lys Gly
1745 1750 1755 1760
Glu Phe Ala Ala Ala His Cys Phe Ala Lys Ser Leu Val Leu Gln Ser
1765 1770 1775
Ile Val Pro Ala Glu Ile Thr Ile Pro Met Leu Ala Gly Ile Asp Phe
1780 1785 1790
Ala Leu Val Ala Ala Thr Lys Ala Thr Ile Val Ile Asp Arg Asp Asn
1795 1800 1805
Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp Gln Asp
1810 1815 1820
Gly Gly Ala Lys Val Val Val Gly Ser Glu Ile Pro Met Lys Val Leu
1825 1830 1835 1840
Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn Asn Gly
1845 1850 1855
Asn Val Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala Cys Ser
1860 1865 1870
Gln Ile Glu Ile Lys Val Gly Asp Ala Glu Thr Met Leu Val Ser Asp
1875 1880 1885
Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser Gln Val
1890 1895 1900
Phe Glu Leu Gly Asp Gly Cys Asp Cys Ser His Lys Thr Gln Ile Gly
1905 1910 1915 1920
Leu Cys Gly Ser Val Gly His Ser Thr His Asp Ile Ala Tyr Ile Ser
1925 1930 1935
His Ala Tyr Leu His Arg Trp Pro Ile Tyr Val Ile Ser Thr Leu Ile
1940 1945 1950
Ala
<210> 15
<211> 2028
<212> PRT
<213> 人工序列(Artificial Sequence)
<400> 15
Met Glu Glu Ala Asp Met Glu Asn Arg Gly Asn Glu Glu Arg His Ser
1 5 10 15
Gln Ser Gln Ser Ala Thr Pro Leu Arg Arg Arg Lys Arg Ser Pro Val
20 25 30
Phe Gly Arg Ser Ser Asp Asp Ile His Ser Phe Ser Lys Asn Pro Glu
35 40 45
Glu Phe Leu Thr Arg Asn Thr Leu Ser Cys Ser Leu Leu Thr Arg Thr
50 55 60
Ala Glu Ala Val Thr Lys Ser Pro Ala Leu Pro Ala Gln Gly Trp Val
65 70 75 80
Lys Val Gly Arg Val Pro Gly Arg Gly Asp Val Leu Phe Val Gln Leu
85 90 95
Val Ala Glu Lys Ser Asp Glu Thr Val Lys Ala Tyr Leu Leu Gly Tyr
100 105 110
Asn Gly Glu Asn Asp Phe Pro Lys Pro Ala Tyr Val Asp Ile Pro Lys
115 120 125
Arg Gly Ala Lys Asn Gly Thr Phe Leu Phe Thr Val Asp Leu Ser Gly
130 135 140
Cys Ser Val Val Val Thr Gln Leu Asp Glu Gln Thr Tyr Arg Val Phe
145 150 155 160
His Asp Lys Arg Val Asn Ser Ser Ile Leu Tyr Asp Asn Val Val Met
165 170 175
Tyr Val Asp Phe Asn His Tyr Arg Tyr Pro Arg Ala His Gly His Gly
180 185 190
Arg Asp Thr Gly Gln Ala Val Val Cys Met Gln Phe Ala Asp Gly Val
195 200 205
Trp Lys Leu Phe Leu Gln Arg His Glu Thr Ala Asn Ser Leu Pro Lys
210 215 220
Ile Trp Ser Arg Gly Asp Gly Lys Pro Glu Ile Phe Ser Asp Ala Asp
225 230 235 240
Pro Arg Pro His Met Glu Asp Gly Ile Lys Glu Phe Leu Asn Arg Arg
245 250 255
Arg Ala Leu Gln Asp Glu Leu Lys Lys Phe Ala Asp Met Phe Gly Val
260 265 270
Asp Pro Glu Ile Val Ser Asn Ala Val Asp Gly Lys Tyr Glu Gly Gly
275 280 285
Gln Phe Ser Arg Asn Asp Asp Ala Ile Arg Gly Trp Asn Glu Leu Arg
290 295 300
Ala Arg Met Arg Gln Lys Met Ala Glu Arg Lys Lys Leu Leu Glu Thr
305 310 315 320
Lys Lys Gly Met Leu Asp Ser Phe Asn Asn Pro Asp His Pro Leu Tyr
325 330 335
Glu Phe Arg Lys Gln Val Leu Asp Glu Leu Lys Glu Met Ser Ala Tyr
340 345 350
Asp Asn Thr Leu Glu Ala Ser Glu Ile Ala Asp Arg Thr Trp Leu Trp
355 360 365
Leu Glu Ile Lys Lys Ala Arg Gly Thr Asn Ala Val Val Asn Ser Gly
370 375 380
Ser Ala Pro Lys Gln Pro Ser Leu Thr Val Gly Glu Arg Tyr Ala Glu
385 390 395 400
Met Glu Ala Ala Leu Trp Met Arg Ser Asp Thr Ala Ala Phe Glu Gln
405 410 415
Gly Tyr Glu Ser Tyr Gln Thr Val Arg Val Pro Gly Tyr Asn Glu Asp
420 425 430
Met Thr Ser Ile Ala Met Lys Lys Leu Phe Val Glu Ser Ser Leu Thr
435 440 445
Asp Glu Gln Leu Gly Ala Leu Cys Lys Arg Ile Ser Ile Ala Ser Asp
450 455 460
Gln Glu Asn His Leu Leu Arg Gln Lys Thr Gln Glu Ile Glu Asn Thr
465 470 475 480
Phe Lys Gln Phe Ser Asp Gly Ser Phe Leu Glu Ser Ala Pro Gln Asp
485 490 495
Phe Phe Leu Arg Arg Val Gly Asp Lys Ser Gly Gly Arg Cys Tyr Pro
500 505 510
Leu Val Arg Val Met Ala Val Ala Leu Ser Gln Gly Glu Asp Val Ala
515 520 525
Gln Asn Phe Met Glu Asn Leu Arg Thr Leu Ser Arg Asn Pro Glu Tyr
530 535 540
Ser Gly Glu Ser Ala Ser Ser Gln Ala Thr Asn Ala Gly Leu Phe Arg
545 550 555 560
Asp Ala Leu Ala Gly Leu His Ala Asn Val Glu Ala Ile Ala Ala Ser
565 570 575
Glu Lys Leu Pro Ala Val Phe Gly Leu Pro Asp Val Ala Thr Met Leu
580 585 590
Ser Gln Ala Ser Glu Ser Lys Val Tyr Ala Leu Asn Thr Glu Thr His
595 600 605
Ser Met Leu Val Ala Val Ile Ala Asp Glu Gln Ser Leu Ala Pro Lys
610 615 620
Tyr Tyr Phe Tyr Asp Pro Asn Phe Leu Met Ala Gly Phe Ser Asp Ile
625 630 635 640
Thr Arg Phe Ser Glu Ala Leu Lys Tyr His Phe Val Asn Leu Gln Phe
645 650 655
Ala Gly Asn Tyr Lys Ala Phe Gly Thr Ala Glu Ala Pro Gln Phe Asn
660 665 670
Leu Val Leu Ile Asp Thr Thr Ala Met Ala Asp Ala His Val Asp Gly
675 680 685
Gly Ile Thr Val Lys Gln Leu Ser Glu Gly Val Asp Leu Thr Asn Val
690 695 700
Phe Asp Ser Thr Lys Lys Ala Arg Val Val Thr Glu Ala Ala Ala Arg
705 710 715 720
Leu Ala Glu Asp Gln Gln Leu Arg Thr Gly Leu Thr Ile Ile Glu Ala
725 730 735
Asp Gln Trp Ala Asn Arg Trp Gln Asp Ala Val Ile Arg Leu Arg Leu
740 745 750
Lys Glu Gly Leu Ser Gly Glu Trp Met Pro Leu Leu Glu Lys Met Gln
755 760 765
Glu Asn Ala Asp Gly Ser Tyr Gln Ile Asp Phe Ile Asn Thr Lys Asn
770 775 780
Thr Gly Glu Ile Arg Gly Val Ser Thr Arg Asp Ala Thr Phe Lys Glu
785 790 795 800
Phe Lys Ser Tyr Leu Asp Lys Asn Leu Asp Ala Leu His Lys Asp Phe
805 810 815
Ile Phe Gln Asp Gly Val Phe Lys Pro Leu Glu Gly Glu Pro Ile Asp
820 825 830
Gly Leu Asn Ala Met Phe Ala Val Gln Met Ile Ile Asp Trp Phe Asn
835 840 845
Arg Gly Thr Asn Ala Glu Glu Asp Ala Ala Ser Gly Ser Thr Asn Ala
850 855 860
Gln Leu Ser Leu Ala Ile Lys Val His Ser Tyr Ile Asn Met Ala Gln
865 870 875 880
Val Ala His Thr Ala Val Thr Asp Val Ala Lys Val Tyr Ser Leu Val
885 890 895
Lys Ser Ala Leu Tyr Gly Glu Gln Val Ala Gly Gly Ala Ser Leu Leu
900 905 910
Thr Phe Gly Lys Ala Leu Lys Phe Ala Ala Thr Glu Gly Leu Gly Val
915 920 925
Leu Leu Gly Ala Ser Phe Val Ile Leu Asp Ala Tyr Gln Leu Ser Leu
930 935 940
Val Glu Asn Asp Val Gln Lys Ala Ile Ile Gly Thr Gln Leu Ala Phe
945 950 955 960
Asp Ser Ala Ser Phe Leu Val Ser Ile Gly Ala Ala Val Ala Gly Val
965 970 975
Ser Gly Val Gly Ile Val Leu Ser Gly Ala Gly Val Leu Leu Ala Gly
980 985 990
Leu Gly Val Gly Phe Gly Ala Leu Ala Gln Ala Phe Gly Gln Ile Thr
995 1000 1005
Ala Asp Ala Gln Leu Val Gly Lys Tyr Phe Ala Leu Leu Asp Glu Ala
1010 1015 1020
Tyr Ser Gly Gly Gly Tyr Arg Tyr Glu Ser Lys Asp Lys Ala Leu Met
1025 1030 1035 1040
Pro Leu Ala Gly Ala Val Ile Asp Lys Ile Arg Leu Ala Asp Lys His
1045 1050 1055
Leu Gln Val His Phe Asp Ser Gln Phe Ile Tyr Arg Thr His His Gly
1060 1065 1070
Ser Thr Gly Ser Gly Lys Ile Asn Tyr Phe Phe Trp Ala Gly Asp Met
1075 1080 1085
Pro Gln Met Val His Asp Arg Ser Gln Ala Ile Asn Val Arg Glu Gln
1090 1095 1100
Ile Gly Tyr Lys Asn Asp Ser Ala Thr Leu Glu Leu Pro Glu Asp Ala
1105 1110 1115 1120
Ser Asp Leu Ile Leu Pro Ala Thr Pro Lys Ser Phe Ile Thr Tyr Arg
1125 1130 1135
Tyr Glu Ile Leu Pro Phe Ala Thr Ser Arg His Asp Arg Gly Phe Asp
1140 1145 1150
Val Ile Arg Arg Leu Glu Glu Asp Tyr Arg Phe Asp Tyr Asp Phe Tyr
1155 1160 1165
Ile Phe Pro Ser Glu Tyr Thr Ile Arg Thr Ile Lys His Glu Tyr Val
1170 1175 1180
Glu Thr Lys Val Ala Val Val Leu Asp Arg Arg Ser Leu Arg Leu Gly
1185 1190 1195 1200
Ala Pro Glu Leu Leu Lys Glu Gln His Gly Tyr Leu Ile Tyr Glu Ile
1205 1210 1215
Glu Gly Thr Gly Gly Gln Tyr Val Val Cys Leu Asn His Gly Ser Thr
1220 1225 1230
Val Thr Leu Lys Thr Thr Gly Ser Glu Ala Asn Lys Thr Leu Trp Ile
1235 1240 1245
Leu Asp Ala Arg Gln Leu Ile Ala Gly Asn Asp Ser Ile Thr Val Ser
1250 1255 1260
Gln Asp Ser Ile Thr Ile Gly Ser Thr Thr Val His Leu Arg Asn Gln
1265 1270 1275 1280
Asp Phe Asp Cys Leu Gln Ile Val Met Ser Arg Glu Glu Leu Leu Gln
1285 1290 1295
Val Asp Leu Ala Ser Arg Gln Ala Arg Val Leu Ser Glu Asp Ala Ser
1300 1305 1310
Lys Trp Leu Asp Thr Lys Glu Thr Ile His Glu His Leu His Lys Leu
1315 1320 1325
Ala Glu Glu His Arg Leu Leu Gly Gln Phe Val Val Val Asn Asn Tyr
1330 1335 1340
Ser Val Gln Glu Gln Ser Asp Ile Gly Thr Leu Tyr Arg Gly Val Gly
1345 1350 1355 1360
Arg Ala Tyr Tyr Asp Ala Ala His Asp Arg Met Leu Tyr Thr Asp Val
1365 1370 1375
Glu Asp Glu Thr Leu Thr Arg Asp Ala Val Leu Ala Gly Val Thr Gly
1380 1385 1390
Glu Asp Ala Tyr Phe Tyr Asn Val Lys Gln Asn Ala Leu Trp Arg Val
1395 1400 1405
Asn Ala Gly Thr His Gln Val Ile Thr Lys Tyr Tyr Pro Thr Val Leu
1410 1415 1420
Glu Asp Pro Asp Ser Lys Met Ala Arg Val Glu Gln His Gly Asn Ile
1425 1430 1435 1440
Val Val Met Ala Met Ser Arg Leu Asn Gln Pro Asp Lys Gly Val Asp
1445 1450 1455
Gly Ile Ile Tyr Leu Val His Gln Glu Ser Met Val Leu Thr Ala Val
1460 1465 1470
Ile Gly Asn Ser Glu Met Leu Lys Arg Leu Arg Gln Thr Ser Thr Ile
1475 1480 1485
Ser Leu Thr Glu Leu Arg Gly Val Asn Asp Met Ala Leu Val Ser Thr
1490 1495 1500
Met Asp Lys Leu Pro Gly Gln Lys Val Val Ala Thr Asn Ala Glu Phe
1505 1510 1515 1520
Val Thr Ile Leu Ala Lys Asp Glu Gln Gly Ser Gln Val Cys Phe Trp
1525 1530 1535
Leu Arg Thr Val Asp Gly Val Leu Ile Lys Pro Ser Ile Gln Pro Pro
1540 1545 1550
Ile Pro Thr Asp Leu Ala Leu Thr Val Ser Ser His Lys Val Ala Gly
1555 1560 1565
Gln Asp Val Phe Tyr Phe Cys Ser Ile Lys Glu Gln Thr Val Tyr Arg
1570 1575 1580
Gln Gln Gly Thr Gly Gln Thr Asn Asp Thr Ser Val Gly Ser Ile Pro
1585 1590 1595 1600
Ile Gln Gly Leu Ser Asn Leu Phe Thr Val Tyr Gly Glu Val Phe Ala
1605 1610 1615
Thr Thr Asp Asp Gly Arg Ile Leu Arg Leu Leu Gly Asp Gly Ser Thr
1620 1625 1630
Tyr Leu Glu Gly Ile Asn Glu His Trp Leu Ile Lys His Pro Lys Trp
1635 1640 1645
Trp Gln Asp Leu Ala Thr Leu Glu Ala Glu Thr Ala Thr Thr Ile Ala
1650 1655 1660
Val Phe Gly Val Arg Asp Pro Gln Asn Ala Val Val Pro Ile Trp Tyr
1665 1670 1675 1680
His Ser Gly Lys Ile Ala Val Ala Ser Ser Lys Leu Tyr Gly Lys Gln
1685 1690 1695
Leu Gln Phe Leu Gly Phe His Ser Asn Asp Gln Ala Trp Leu Phe Asp
1700 1705 1710
Leu Asp Gly Gly Ile Leu Tyr Ser Gln Pro Leu Val Asp Arg Gln Thr
1715 1720 1725
Val Asp Thr Ile Phe Ser Leu Asp Gly Lys Leu Ile Ala Pro Asp Leu
1730 1735 1740
Ile Pro Gln Val Gln Gln Met Leu Ser Gly Gln His Leu Arg Asn Pro
1745 1750 1755 1760
Val Ile Val Asp Gly Arg Leu His Val Thr Thr Ile Asp Gly Val Val
1765 1770 1775
Val Gly Phe Asp Lys Lys Ser Pro Thr Leu Val Ala Val Asn Lys Ala
1780 1785 1790
Trp Gln Ile Ser Arg Gln Asn Arg Leu Glu Glu Ala Leu Gly Asn Leu
1795 1800 1805
Leu Ser Asn Trp Ser Asp His Asn Asp Ala Ile Val Leu Gln Gly Asp
1810 1815 1820
Pro Glu Ser Ala Pro Ala Trp Tyr Tyr Val Pro Ser Arg Glu Ile Val
1825 1830 1835 1840
Thr Ala Asn Gly Leu Thr Trp Ala Asp His Pro Leu Trp Leu Gly Met
1845 1850 1855
Ala Val Gly Ala Ala Thr Pro Tyr Ile Tyr Asn Thr Ser Pro Arg Arg
1860 1865 1870
Arg Ser Ile Val Pro Glu Glu Ile Thr Ile Pro Thr Leu Ala Gly Ile
1875 1880 1885
Asp Phe Ala Leu Val Ala Ala Thr Asn Ala Thr Ile Val Ile Asp Arg
1890 1895 1900
Asp Asn Trp Lys His Tyr Lys Ala Ile Val Leu Asp Asn Lys Val Asp
1905 1910 1915 1920
Gln Asp Gly Gly Ala Lys Val Val Leu Gly Ser Glu Ile Pro Met Lys
1925 1930 1935
Val Leu Val Arg Lys Phe Gln Gly Asn Leu Glu Phe Phe Asp Pro Asn
1940 1945 1950
Asn Gly Asn Val Val Ile Leu Gln Asn Val Leu Lys Gly Asp Gly Ala
1955 1960 1965
Cys Cys Gln Ile Asp Ile Lys Val Gly Asp Ala Glu Thr Met Leu Ala
1970 1975 1980
Ser Asp Phe Ser Glu Ile Phe Ser Lys Thr His Pro Leu Val Ala Ser
1985 1990 1995 2000
Gln Val Phe Glu Leu Gly Asp Val Ile Ala Val Thr Lys His Lys Ser
2005 2010 2015
Asp Tyr Glu Asp Leu Trp Ala Ile Leu His Met Thr
2020 2025
<210> 16
<211> 51
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
<210> 17
<211> 93
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
gggcggtacc ccgggttcga acactagtta attaagacgc gt 42
<210> 18
<211> 16379
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 60
acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 120
actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 180
gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctagagcag 240
cttgccaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc 300
tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc 360
ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc 420
acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac 480
agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca 540
accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga cactctcgtc 600
tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa 660
caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc 720
aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag 780
gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg 840
agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat 900
atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga ccttcctcta 960
tataaggaag ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa 1020
atctatctct ctcgagtcta ccatggctca gatcaggtcg atggctcagg gcattcagac 1080
gctgtcgctc aactcctcta atctctccaa gacgcagaag gggccgctcg tgtcgaactc 1140
tctcttcttc ggcagcaaga agctgacaca gatctctgcc aagtcactgg gggttttcaa 1200
gaaggactcg gtgctccggg tggtccgcaa gtccagcttc cgcatctcag cttccgtcgc 1260
tacagctgag gctgaggccg tgatcgctga ggtctccact cagctcagcg aggttgtggg 1320
cgtgatcgag aggcacctgg agccaaccct cctggctgtc catctctacg ggtcagcggt 1380
tgatggtggc ctgaagcccc actccgacat cgatctcctg gttacagtga ctgtccggct 1440
ggacgagacc acgaggaggg ctctcattaa cgatctcctg gagaccagcg cttcgccagg 1500
cgagtccgag atcctcaggg cggttgaggt gacgattgtc gttcatgacg atatcattcc 1560
atggaggtac ccagctaaga gggagctcca gttcggcgag tggcagcgca atgacatcct 1620
ggccgggatt ttcgagccag cgacaatcga cattgatctg gctatcctcc tgactaaggc 1680
tagggagcac tccgtcgctc tggttggccc tgctgctgag gagctcttcg acccagtccc 1740
tgagcaggat ctcttcgagg ccctgaacga gaccctcacg ctgtggaatt ctccgcccga 1800
ctgggctggc gatgagagga atgtggtcct caccctgtcg cgcatctggt actctgctgt 1860
cacggggaag attgctccaa aggacgtggc tgctgattgg gcgatggaga ggctgccagc 1920
tcagtaccag cctgtgatcc tcgaggctag gcaggcttac ctgggccagg aggaggacag 1980
gctcgcgtcc cgggctgatc agctggagga gttcgttcat tacgtcaagg gcgagattac 2040
taaggtcgtt ggcaagtgac tcgagtttct ccataataat gtgtgagtag ttcccagata 2100
agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt 2160
atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat 2220
ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg 2280
tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc 2340
caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag 2400
gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc 2460
tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat 2520
gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca 2580
ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt 2640
tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt 2700
cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa 2760
taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc 2820
tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat 2880
gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa 2940
cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac 3000
tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc 3060
tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc 3120
atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc 3180
cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac 3240
tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa 3300
aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg 3360
aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg 3420
tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc 3480
gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt 3540
ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt 3600
tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 3660
tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 3720
ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 3780
ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 3840
gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3900
aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3960
ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4020
agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4080
aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4140
aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4200
ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4260
cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 4320
tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 4380
accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc 4440
cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct 4500
gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg 4560
cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 4620
ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 4680
catcaccgaa acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac 4740
ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg 4800
ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg 4860
gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg 4920
cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc 4980
tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca 5040
atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca 5100
ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc 5160
gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg 5220
atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg 5280
atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg 5340
tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg 5400
tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg 5460
tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg 5520
tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc 5580
tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc 5640
gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct 5700
cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct 5760
cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc 5820
aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg 5880
tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa 5940
acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg 6000
aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc 6060
ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg 6120
cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc 6180
aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgtcacgc 6240
cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct 6300
tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg 6360
ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc 6420
atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct 6480
tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt 6540
tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc 6600
gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc 6660
attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc 6720
gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct 6780
cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc 6840
aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag 6900
ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca 6960
tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga 7020
cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat 7080
ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc 7140
ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac 7200
atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga 7260
cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta 7320
tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca 7380
catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc 7440
gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc 7500
gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg 7560
aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat 7620
tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc 7680
gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc 7740
gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct 7800
cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc 7860
gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact 7920
cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc 7980
acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca 8040
gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag 8100
ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct 8160
tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt 8220
cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca 8280
tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga 8340
caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg 8400
acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc 8460
ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata 8520
caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgttat tctaataaac 8580
gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg 8640
aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct 8700
gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 8760
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 8820
ttgtaaaacg acggccagtg ccaagctaag cttgcatgcc tgcaggtccc cagattagcc 8880
ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac gcagcaggtc 8940
tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg 9000
ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat 9060
ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa 9120
ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg 9180
agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca 9240
tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg 9300
acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg 9360
agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct 9420
gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg 9480
ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc 9540
cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg 9600
attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag 9660
acccttcctc tatataagga agttcatttc atttggagag aacacggggg actctagagg 9720
atcgcgaggc gcgccgtcga catggactac aaggacgacg atgataaggg catggactac 9780
aaggacgacg atgataaggg catggactac aaggacgacg atgataaggg cggtaccccg 9840
ggttcgaaat ggttatggta attgactatc caagtctcag agcttcgtta tatgccacta 9900
agagtgcggt cccatgtcgc ccgcttccgg gcaatccaga gcgggtgtcg cgccgggtat 9960
cacgcagagg cgcgggagag atcgggtccc atgcagattt ctggatctcc cgcgccaagg 10020
catccgccat ccggattttc ccgcgtcaag agggtccgct ccgtgaatcc cggagaagat 10080
tctatcttct ccgcgaagcg gtgttcaccg gccgcaggac cgcctgggcc gctaatccag 10140
cggcccaacg ggcagcatgg gaccgcaaga gatccccttt cttcggaagg tcttccgatg 10200
atattcattc attcagcaag aatccagaag aattcttgac tcaaaacact ctcagctgca 10260
gtttattaac tcgcaccgct cagaccgtca caaaaagccc agtattgccc gcccaaggtt 10320
gggtcaaagt gggtcgtgtg ccaggaagag gagacgctct ctttgttgag ctggttgccg 10380
agaaatcaga tgagacagtg aaggcgtact tacttggcta caatggtaag aacgactccc 10440
ccgagccggc atacgttgat attccaaaga ggggcgccga gaacggaaca tttttgttta 10500
ccgtggatct ctcgggctgc tccgttgttg tgacacagtt ggacgagcag acataccgtg 10560
ttttccacga caaacgggtg aacagctcca ttctgtacga caacgtcgta atgtatgtgg 10620
atttcaatca ctaccgctat cccagagccc acggacacgg gagagatacg ggacaagcgg 10680
tcgtctgtat gcaattcgcc gacggagtgt ggaaattgtt tctccagcgg cacgagacgg 10740
cgaattctct cccaaagata tggtcccgtg gcgatggcaa accggagatt ttcagcgacg 10800
ccgaaccccg tccacatatg gaagatggca taaaggaatt tctcaatcgg cgtcgagctt 10860
tgcaagacga attgaagcac ttcgccaaca cgcttgaagt ggacccggac atagtgtcca 10920
acgctgtcga cggcgaatac gaaggcggcc agttttccag gaacgacgat gcaattcgtg 10980
gatggaacga attacgtgca agcatgagac agaaaatggc tgaaaggaag aaactgttgc 11040
aaacaaagaa aggtatgcta gactcattca acaacccgga tcaccccctc tataaatttc 11100
gcaaacaagt gctcgacgag ctcaaggaaa tgagtgcata cgacaacacc ttggaggcat 11160
ccgagattgc ggaccgtact tggctgtggt tggagatcaa aaaagccaaa ggtacgaatg 11220
cagtggtaaa cagtggagca gccccgaagc agccgagtct taccgttggt gaacgctacg 11280
cagagatgga ggcggccctt tggatgcgta gcgacacagc ggcgtttgag caagggtatg 11340
aatcttatca aaccgttcgc gtgccgggtt acaatgaaga tatgacatcc attgcaatga 11400
agaagttgtt cgttgagagc agtctgactg acgagcagct gggcgccttg tgcaagcgta 11460
tatccattgc tagcgatcaa gagaaccatc ttctgacaca gaagacgcag gagattgaga 11520
gcactttcaa acaattttca gatggcagct ttttggagtc ggcgcctcag gatttcttcc 11580
tgagacgagt cggtgataaa tctggaggac gctgctatcc tcttgtaagg gtaatggccg 11640
tcgcactgtc tgaaggagaa gacgttgccc agaattttat ggagaacctg aggacattgt 11700
ctaggaatcc agagtattca ggcgaatcgg caagttcaca agccacaaat gcagggttgt 11760
tcagagatgc cctggccggc ctgcacgcga atgtggaagc cattgccgca tctgagaaac 11820
tacctgctgt gtttggactg cccgatgttg ctacaatgtt gagccaagcg agcgaaagca 11880
aggtttacgc tttgaatacc gaaacacaca gcatgctggt tgcggtgatt gtagacaaac 11940
agagtctggc gcgcaagtat tacttctatg atcccaactt tctcatggcc ggtttctccg 12000
acataactcg tttctcggaa gccctcaagt atcactttgt gaatctccag ttcgccggaa 12060
actacaaagc gtttggcaca gcggaggctc cccagtttaa cctggtcctt attgatacga 12120
ccgccatgga ggatgcgcat gttgacggag ggatcaccgt gaaacaattg agcgagggcg 12180
ttgatcttac taatgtcttt gacagcacca aaaaggctcg tgtggtaact gaggcagcag 12240
ctcgcttggc tgaagaccaa caactgcgca ctggcctcac cataatagag gctgaccagt 12300
gggcaaacag gtggcaagat gccgtcatac gattgaaact caaggaagga ttaagtggag 12360
agtggatgcc attgctggag aaaatgcaag aaaacgcaga tggttcttat cagattgatt 12420
tcatcaacac caagaatact ggggaaatcc gtggggtttc cactagagat gcgacgttca 12480
aagaattcaa atcctacctg gataaaaatt tagacgtatt gcataaggat ttcatctttc 12540
aggatggagt tttcaaacca ctggagggag agcccattga cgggttgaat gcaatgttcg 12600
cggtccaaat gattatagat tggttcaacc gggggactaa tgctgaggaa gatgcggcct 12660
caggatccac caatgcccaa ctttccctag cgattaaagt gcacagctac attaacatgg 12720
cccaagtagc gcatactgca gttacagatg ttgccaaggt atacaacctt gtaaagtcag 12780
cactgtatgg cgagcaggta gctggggaga catcgctgtt cacatttggg aaggcgttga 12840
aatttgcagc aacagaaggg ttgggagtct tactaggggc gtcctttgtg atcttggatg 12900
cctaccagct gtccctcgtg gaaaacgatg tgcagaaggc cattatcggc acacaactag 12960
cctttgattc cgcaagcttt ctagcgggta ttggtgcaat tgttgctgga gcatcgggcg 13020
tgggcatgat tttgggcgga gctggcgtgg tattgggagg cctgggagtt ggctttggag 13080
ccttagctca ggcattcggg gaaatcacag cagacgccca actggtgggc aagtacttcg 13140
cactcttgga cgaggcatac cgcggtggcg gatacagata cgaatctaaa gacaaggcat 13200
tgatgcctct tgcaggagca gtcatcgaca aaatacgact ggccgacaaa catctccaag 13260
ttcatttcga cagccaattc atatatcgaa cccatcatgg cagcacagga tccggcaaaa 13320
ttaattactt cttctgggct ggcgacatgc cgcagatggt ccacgatcgc tcccaagcta 13380
tcaatgttcg agagcaaatt gggtataaga acgataatgc tactttggag ctaccggagg 13440
atgcctctga tctgatcctg cctgccacgc ccaagtcatt tatcacttac cgatacgaga 13500
ttctaccatt cgctacttcc cgccacgata cgggtttcga tgtcattaga cgcctggaag 13560
aagactacag attcgattac gatttctaca tcttcccatc tgaatacaca atcagaacca 13620
tcaaacacga gtacgtagaa acaaaggtgg cagtagtcct ggataggcgc tctttgcgac 13680
tgggagcacc tgagcttctc aaggagcaac atggctattt gatctatgag atcgagggga 13740
ccggaggaca atacgttata tgtcttaacc agggatccac tgtcacactg aagaccaccg 13800
gatcagaggc aaataaaaca ttatggatac ttgatgcccg acaactcatt gcaggcaacg 13860
attcaatcac ggtctctcaa gatagcatca ccatcggcag cacaacagtc catctccgta 13920
accaagattt cgattgccta cagatcgtca tgagtcggga ggagcttctc caagtggact 13980
tggcgtctgg agaagcccgc gtgttttcag aagatgccag taaatggctc gacaccaaag 14040
aaacaatcca tgaacatctc cataagttgg ctgaagaaca caggttgcat ggccagtttg 14100
tagtggtgaa caattattat cgtggagtgg gccgtgccta ctatgatgct gctcacgatc 14160
ggatgttgta tacagatgtg gaagatgaaa ctctgactcg ggatgctgtt cttgccggcg 14220
ttacaggcga agatgcctac ttctacaatg taaagcagaa tgccctgtgg cgagtgaatg 14280
caggcacaca ccaagtaata accaaatact atccgacgtt actggaggac cctgacagca 14340
acatggcaag agttgagcag catggcaaca tcgtcgtgat ggccatgagc aggctcaacc 14400
acccggacaa gggagtaaat ggaatcatct atctcgttca tcaagaaagc atcgtgctta 14460
ctgctgtgat cggcaattca gagatgctca aacgtcttcg ccaaacgagt acaattagct 14520
taacagaatt gcgaggtgtc aatgacatgg ctctggtttc cacgatggac agattacccg 14580
gccaaaaggt tgtcgctacc aacgctgagt ttgtgacact tctggctaag gatgagcaag 14640
gagccaatat caaggagcag acggtctatt gccaacaagg tacgggccag accaacgata 14700
catctattga aagcatcccg attccagggc tctctaatct tttaaccgtg tacggtgaag 14760
tattcgctac aactaatgat ggtcgcatcc ttcgcctgct tggggacggc tcaacatgtc 14820
tcgagggcat caatgagcat tggctcatca agcatccaaa atggtggcag gacttggcta 14880
ccttggaggc agagaaagcc acgacagttg cagtattcgg tgttagagac ctacaaaatg 14940
cagtggtgcc aatttggtac cacaacggaa agatcgcggt ggcctcatca aaactgtatg 15000
gcaaacaact gcaatttttg gggtttaaca gcaacggtga agcttggttg ttcgacttgg 15060
acgacggcat tctgtatagc cagccgcttg tggatagctt tctgtatgca atctttagtt 15120
tggacgggaa gcttattgca ccaatcttta ttccacaagt ggagcaaatg ctcagcggcc 15180
agcatctcag aaaccctgtc atcgtggacg gccgtttgca tgtgactacc atagacggtg 15240
ttgttgttgg atttgacaag aagtcgccaa ctctggttgc agtgaacaag gcctggcaga 15300
tatctcgtca gaaccgatta gaagaggccc tcggaaacct gctgtccaat tggtctgatc 15360
acaatgatgc gattgtgttg cagggtgatc cagaatcagc gccggcatgg tattatgttc 15420
catcaagaga aatagtgacc gccaatggtc taacatgggc ggatcatcct ctatggctag 15480
gaatggctgt cggtgctgca acgccataca tctacagcac atcccgacgc cgtgtgtatg 15540
ttgtggagag caaaaacatg gccaaagaca aaggagaatt cgcagcagca cattgctttg 15600
caaaatcact tgtgctacag agcatcgttc ccgaggaaat aacgattccg acgctggctg 15660
gcatcgattt cgcactcgtt gcagcgacaa atgcaaccat tgtaatagac cgagacaatt 15720
ggaagcatta caaggccatt gtgctcgaca acaaggtgga tcaggacggt ggcgctaagg 15780
tggtagtcgg cagtgagatt cccatgaaag ttctggtgcg caaatttcag ggaaatctgg 15840
agttcttcga tccaaacaat ggtaatgttg ttatactcca gaatgtgttg aagggagacg 15900
gagcttgttg ccagatagag atcaaagtag gcgatgctga gactatgctt gtatccgatt 15960
tctcggagat attctcaaag acgcatcctc tggtggcaag ccaagtattt gagcttgggg 16020
atgtgattgc agtcacaaaa cacaaatcgg actatgcgga tctgtgggcc attctacgca 16080
tgacatagac gcgtcttaat taactagtga gctcgaattt ccccgatcgt tcaaacattt 16140
ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat 16200
ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga 16260
gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa 16320
tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcgg 16379
<210> 19
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19

Claims (5)

1.一种抗虫性的苏铁毒蛋白基因，其特征在于，所述抗虫性的苏铁毒蛋白基因的核苷酸序列为SEQ ID NO.1或SEQ ID NO.6。
2.一种如权利要求1所述的抗虫性的苏铁毒蛋白基因构建得到的植物表达载体，其特征在于，所述植物表达载体的构建方法包括：
线性化载体制备：使用限制性内切酶BstBI和MluI双酶切VM062-3*FLAG载体，使环状载体线性化；
带有同源臂的目标序列扩增：
以正向引物：
5’-gggcggtaccccgggttcgaaATGGTTATGGTAATTGACTATCCAAGTC-3’；反向引物：
5’-cactagttaattaagacgcgtCTATGTCATGCGTAGAATGGCCC-3’为引物，对苏铁毒蛋白基因进行PCR扩增；
载体酶切产物与PCR产物的检测与回收：琼脂糖凝胶电泳检测目的基因片段与载体酶切产物，使用Quick Gel Extraction Kit回收目的片段；
胶回收产物重组：使用-Basic Seamless Cloning and Assembly Kit进行同源重组，反应体系如下：2×Basic Assembly Mix 5μL；酶切载体XμL；PCR产物YμL；将灭菌水加入至10μL，10μL反应体系中，载体与各个***片段加入量均在0.01～0.25pmols，载体与各个***片段的最佳摩尔比为1：2；轻轻混合，50℃反应15min，进行转化；
测序鉴定：经菌落PCR和质粒PCR筛选阳性克隆，进行测序分析。
3.一种如权利要求1所述的抗虫性的苏铁毒蛋白基因编码的蛋白质，其特征在于，所述蛋白质的氨基酸序列为SEQ ID NO.11。
4.一种如权利要求1所述的抗虫性的苏铁毒蛋白基因的杀虫应用或杀虫剂制造，应用范围为棉铃虫、小菜蛾、草地贪夜蛾的植物害虫。
5.一种应用如权利要求1所述的抗虫性的苏铁毒蛋白基因的植物的抗虫方法，其特征在于，所述植物的抗虫方法包括：
将含有目标序列的植物表达载体导入到植物细胞内，并由此获得对害虫有抗性或杀伤能力的转基因植物及其后代，包括种子和任何部分的植物组织；或者通过同源或异源表达的方法获得该蛋白施用于植物，害虫为棉铃虫、小菜蛾、草地贪夜蛾的植物害虫。
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