CN114847418A - Composite fat source for improving muscle quality of tilapia based on accurate nutritional requirement of fatty acid - Google Patents
Composite fat source for improving muscle quality of tilapia based on accurate nutritional requirement of fatty acid Download PDFInfo
- Publication number
- CN114847418A CN114847418A CN202210570656.1A CN202210570656A CN114847418A CN 114847418 A CN114847418 A CN 114847418A CN 202210570656 A CN202210570656 A CN 202210570656A CN 114847418 A CN114847418 A CN 114847418A
- Authority
- CN
- China
- Prior art keywords
- tilapia
- fatty acid
- oil
- fat source
- muscle quality
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to the field of aquatic animal nutrition and feed, and particularly relates to a method for improving the muscle quality of tilapia based on the accurate nutritional requirement of fatty acid; comprises the following fat source components: soybean oil, lard oil, tea seed oil, linseed oil; the invention aims to establish a set of precise fatty acid nutrition regulation and control technology for tilapia muscle quality and provides a composite fat source for healthily and effectively improving the tilapia muscle quality.
Description
Technical Field
The invention belongs to the field of nutrition and feed of aquatic animals, and particularly relates to improvement of tilapia muscle quality based on accurate nutrition demand of fatty acid.
Background
Tilapia (Oreochromys mossambicas) has the characteristics of delicious meat, rapid growth, short culture period and the like. In 2020, the annual yield of tilapia reaches 165 million tons, wherein the annual yield of Guangdong province reaches 74 million tons, and the tilapia becomes important cultured fish and main export water products in Guangdong province. At present, with the development of high-density intensive culture, the serious problems of low muscle hardness and chewiness, poor meat quality and mouthfeel and the like of cultured tilapia generally exist, and the cultured tilapia becomes one of the most important problems of the tilapia industry. It has been reported that, although the yield of tilapia mossambica is rising year by year, the tilapia mossambica has decreased muscle-structure characteristics, loose meat, decreased hardness and chewiness, and poor taste, resulting in a lower price and a lower profit margin for cultivation (Dey et al, 2000). Therefore, a small number of farmers imitate the production method of crisped grass carp, and adopt broad bean products to improve the meat taste of tilapia so as to improve the culture benefit (the crisp tilapia pond price is up to 12 yuan/jin). However, compared with grass carp, tilapia has low tolerance to broad bean products, the addition amount of the tilapia is difficult to control, the tilapia is easy to die, and the tilapia is not easy to embrittle when the addition amount is too high.
In order to find a method for safely and effectively improving the muscle and texture characteristics of cultured tilapia, in recent years, scientific and technical workers at home and abroad carry out related research on the basis of plant extracts. For example, addition of mannooligosaccharides to high-sugar feed (sugar content 45%) can improve the meat yield, muscle water retention, muscle taste and amino acid nutritive value of tilapia (maricrystal, et al, 2021). Under the condition of supplementing 0.3 percent of gentian extract, the contents of crude protein, essential amino acid and umami amino acid of tilapia muscle can be obviously improved (Zhaoyao et al, 2021). However, there is no report on the research of improving the muscle quality of the cultured fish based on the nutritional requirement of fatty acid.
Aiming at the industrial problems, the invention adopts soybean oil, lard oil, tea seed oil and linseed oil to form composite oil, optimizes the fatty acid composition of the feed by adjusting the proportion of each oil, focuses on the research of accurate nutrition regulation and control of the feed fat and the fatty acid, and develops a composite fat source capable of improving the muscle quality of the tilapia. The invention has important significance for improving tilapia consumption and breeding industry development.
The main references:
(2021) the effect of mannooligosaccharides on the quality of flesh of Niluofia fish fed with high sugar.
Zhao, chen peiyi 36191, chenhui qin, (2021) effect of gentian extract on muscle quality of gift tilapia, jiang xi aquatic science (06),4-9.
Li,Y.,Zhang,J.,Fu,B.,Xie,J.,et al.(2022).Textural quality,growth parameters and oxidative responses in Nile tilapia(Oreochromis niloticus)fed faba bean water extract diet.[J].PeerJ,10.
Dey,MM.,Bimbao,GB.,Yong,L.,et al.(2000).Current status of production and consumption of tilapia in selected Asian countries.Aquaculture Economics&Management,4,13–31.
Disclosure of Invention
The invention proposes: based on the accurate nutritional requirement of fatty acid, a safe and effective composite fat source capable of improving the muscle quality of tilapia is developed.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the composite fat source for improving the muscle quality of tilapia based on the accurate nutritional requirement of fatty acid comprises the following fat source components: soybean oil, lard oil, tea seed oil and linseed oil.
Further, the fat source comprises the following fat sources and fatty acids thereof in percentage by mass: 0.0 to 8.0 percent of soybean oil, 0.0 to 3.0 percent of lard oil, 0.0 to 3.0 percent of tea seed oil and 0 to 5.0 percent of flaxseed oil; 15.0-25.0% of Saturated Fatty Acid (SFA), 25.00-35.00% of monounsaturated fatty acid (MUFA), 6.00-28.00% of n-3 polyunsaturated fatty acid (PUFA) and 15.00-50.00% of n-6 PUFA.
Further, the fat source comprises the following fat sources and fatty acids thereof in percentage by mass: 3.0% of soybean oil, 1.0% of lard oil, 0.5% of tea seed oil and 3.5% of linseed oil; SFA 20.00%, MUFA 20.00%, n-3PUFA 25.00%, n-6PUFA 35.00%.
Drawings
FIG. 1 is a muscle section (HE staining, 40X) of tilapia in the experimental group of example 1;
FIG. 2 is a tilapia muscle section (HE staining, 40X) of the control group in example 3.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the feed consists of the following feed grease and fatty acid components in percentage by weight: 3.0% of soybean oil, 1.0% of lard oil, 0.5% of tea seed oil and 3.5% of linseed oil, adding the oils according to corresponding proportions, homogenizing and uniformly mixing; SFA 20.0%, MUFA 20.0%, n-3PUFA 25.0%, n-6PUFA 35.0%.
Example 2:
the feed consists of the following feed grease and fatty acid components in percentage by weight: 0.0% of soybean oil, 3.0% of lard oil, 3.0% of tea seed oil and 2.0% of linseed oil, adding the oils according to corresponding proportions, homogenizing and uniformly mixing; SFA 25.0%, MUFA 35.0%, n-3PUFA 15.0%, n-6PUFA 15.0%.
Example 3:
the feed consists of the following feed grease and fatty acid components in percentage by weight: 8.0% of soybean oil, 0.0% of lard oil, 0.0% of tea seed oil and 0.0% of linseed oil, adding the oil in corresponding proportion, homogenizing and uniformly mixing; SFA 15.0%, MUFA 29.0%, n-3PUFA 6.0%, n-6PUFA 50.0%.
Test subjects: selecting 300 healthy tilapia mossambica with the specification of 40 g/strip, and dividing the tilapia mossambica into a test group and a control group randomly, wherein each group contains 100 tilapia mossambica.
The test method comprises the following steps: the test group was fed with the mixed oil of the invention example 1 to prepare a feed for tilapia, and the control group was fed with the feed prepared from the mixed oil of the example 3. The culture period in the pond net cage (1.5m × 1.5m × 2m) is 10 weeks, and the same culture management is carried out on the test group and the control group during the culture period.
After the culture is finished, the tilapia mossambica in the test group and the control group are fasted for 24 hours, are weighed one by one for length measurement, record the weight and the body length, and statistically analyze the growth performance indexes such as the terminal weight, the weight gain rate, the specific growth rate and the like and the fullness. 3 fishes are respectively taken from the test group and the control group, after being dissected, the viscera and the liver are weighed, and the viscera volume ratio and the liver volume ratio are statistically analyzed; then, 3-5g of muscle sample is put into a centrifuge tube, and is quickly frozen by liquid nitrogen, and then is stored in a refrigerator at-80 ℃ for later use to measure the fatty acid composition; another muscle sample with the size of 1cm is taken and stored in the stationary liquid to be sliced and analyzed.
Technical effects
Compared with a control group, the composite fat source for improving the muscle quality of tilapia based on the accurate nutritional requirement of fatty acid has no statistical difference (P is more than 0.05) in growth performance such as the weight gain rate, specific growth rate, feed coefficient and the like and feed utilization rate indexes of the invention group. The body fullness of the fish in the invention group is obviously higher than that of the control group (P <0.05), and the muscle collagen content of the fish is improved by 19.20 percent (P < 0.05). Compared with the control group feed, the muscle fiber of the invention group fish has small diameter and high density; the texture characteristics such as muscle hardness, elasticity, chewiness and the like are obviously improved (P is less than 0.05), and the meat taste is improved. In addition, the content of n-3 polyunsaturated fatty acid (ALA, EPA and DHA) in the muscle of the invention group fish is obviously improved, and volatile flavor substances such as nonanal, dodecanal, tetradecanal, 1-octanol and the like are obviously improved (P < 0.05).
And (3) test results: tilapia growth index (table 1), muscle nutrient composition (table 2, table 3), muscle texture characteristics and flavor (table 4, table 5); the muscle sections of the test group and the control group are shown in detail in FIGS. 1 and 2.
TABLE 1 Tilapia growth Performance index
TABLE 2 Effect of conventional ingredients and physical Properties of Tilapia muscle
TABLE 3 fatty acid composition of muscle of Tilapia mossambica
TABLE 4 Tilapia muscle volatile flavor levels
TABLE 5 Tilapia muscle texture characteristics
Claims (3)
1. A composite fat source for improving the muscle quality of tilapia based on the accurate nutritional requirement of fatty acid is characterized in that: comprises the following fat source components: soybean oil, lard oil, tea seed oil and linseed oil.
2. The composite fat source for improving tilapia muscle quality based on fatty acid precision nutritional requirements according to claim 1, characterized in that: the fat source and the fatty acid thereof comprise the following components in percentage by mass: 0.0 to 8.0 percent of soybean oil, 0.0 to 3.0 percent of lard oil, 0.0 to 3.0 percent of tea seed oil and 0 to 5.0 percent of flaxseed oil; 15.0-25.0% of Saturated Fatty Acid (SFA), 25.00-35.00% of monounsaturated fatty acid (MUFA), 6.00-28.00% of n-3 polyunsaturated fatty acid (PUFA) and 15.00-50.00% of n-6 PUFA.
3. The composite fat source for improving tilapia muscle quality based on fatty acid precision nutritional requirements according to claim 1, characterized in that: the fat source and the fatty acid thereof comprise the following components in percentage by mass: 3.0% of soybean oil, 1.0% of lard oil, 0.5% of tea seed oil and 3.5% of linseed oil; SFA 20.00%, MUFA 20.00%, n-3PUFA 25.00%, n-6PUFA 35.00%.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210570656.1A CN114847418B (en) | 2022-05-24 | 2022-05-24 | Composite fat source for improving muscle quality of tilapia based on accurate nutrition requirement of fatty acid |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210570656.1A CN114847418B (en) | 2022-05-24 | 2022-05-24 | Composite fat source for improving muscle quality of tilapia based on accurate nutrition requirement of fatty acid |
Publications (2)
Publication Number | Publication Date |
---|---|
CN114847418A true CN114847418A (en) | 2022-08-05 |
CN114847418B CN114847418B (en) | 2023-08-22 |
Family
ID=82640269
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210570656.1A Active CN114847418B (en) | 2022-05-24 | 2022-05-24 | Composite fat source for improving muscle quality of tilapia based on accurate nutrition requirement of fatty acid |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114847418B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115500433A (en) * | 2022-09-27 | 2022-12-23 | 中国水产科学研究院黄海水产研究所 | Method for balancing fatty acid in fish feed, feed and application |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106721655A (en) * | 2016-12-21 | 2017-05-31 | 怀宁县鲶鱼湾生态农业有限公司 | Catfish scrod feed and its manufacture method |
CN106721526A (en) * | 2016-11-29 | 2017-05-31 | 河南科技大学 | A kind of the Yellow River carp anti-stress feed and preparation method thereof |
CN106923071A (en) * | 2015-12-29 | 2017-07-07 | 青岛智通四海家具设计研发有限公司 | A kind of health care feed of grass carp |
CN109198299A (en) * | 2018-10-26 | 2019-01-15 | 南宁学院 | It is a kind of regulate and control red Rofe postlarva fatty acid metabolism feed and preparation method thereof |
CN109258933A (en) * | 2018-03-27 | 2019-01-25 | 厦门福慈生物科技有限公司 | Fullerene feeding additive aquatic animal and preparation method thereof and feed |
CN110150511A (en) * | 2019-07-08 | 2019-08-23 | 河南牧业经济学院 | A kind of freshwater fish quality improving agent and preparation method thereof |
CN112075549A (en) * | 2020-09-14 | 2020-12-15 | 湖北优百特生物工程有限公司 | Fat powder special for freshwater fish and preparation method and application thereof |
CN112262795A (en) * | 2020-11-06 | 2021-01-26 | 中国水产科学研究院黄海水产研究所 | Nutrition method for maintaining muscle fatty acid quality of low-fat type cultured fish |
CN113475643A (en) * | 2021-07-02 | 2021-10-08 | 广州观星农业科技有限公司 | Functional compound feed for tilapia and preparation method thereof |
-
2022
- 2022-05-24 CN CN202210570656.1A patent/CN114847418B/en active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106923071A (en) * | 2015-12-29 | 2017-07-07 | 青岛智通四海家具设计研发有限公司 | A kind of health care feed of grass carp |
CN106721526A (en) * | 2016-11-29 | 2017-05-31 | 河南科技大学 | A kind of the Yellow River carp anti-stress feed and preparation method thereof |
CN106721655A (en) * | 2016-12-21 | 2017-05-31 | 怀宁县鲶鱼湾生态农业有限公司 | Catfish scrod feed and its manufacture method |
CN109258933A (en) * | 2018-03-27 | 2019-01-25 | 厦门福慈生物科技有限公司 | Fullerene feeding additive aquatic animal and preparation method thereof and feed |
CN109198299A (en) * | 2018-10-26 | 2019-01-15 | 南宁学院 | It is a kind of regulate and control red Rofe postlarva fatty acid metabolism feed and preparation method thereof |
CN110150511A (en) * | 2019-07-08 | 2019-08-23 | 河南牧业经济学院 | A kind of freshwater fish quality improving agent and preparation method thereof |
CN112075549A (en) * | 2020-09-14 | 2020-12-15 | 湖北优百特生物工程有限公司 | Fat powder special for freshwater fish and preparation method and application thereof |
CN112262795A (en) * | 2020-11-06 | 2021-01-26 | 中国水产科学研究院黄海水产研究所 | Nutrition method for maintaining muscle fatty acid quality of low-fat type cultured fish |
CN113475643A (en) * | 2021-07-02 | 2021-10-08 | 广州观星农业科技有限公司 | Functional compound feed for tilapia and preparation method thereof |
Non-Patent Citations (3)
Title |
---|
叶儒锴等: "液态和粉末脂肪对吉富罗非鱼幼鱼生长、健康及肌肉品质的影响", vol. 43, no. 10, pages 2197 - 2208 * |
岳彦峰;彭士明;施兆鸿;尹飞;孙鹏;: "脂肪酸营养对鱼类生长、脂代谢及免疫性能影响的研究进展", no. 11, pages 13 - 19 * |
王婧瑶;吴莉芳;段晶;瞿子惠;杨兰;周锴;王桂芹;: "饲料中必需脂肪酸对鱼类生长、抗氧化能力及脂肪酸代谢酶活性的影响", no. 09, pages 16 - 20 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115500433A (en) * | 2022-09-27 | 2022-12-23 | 中国水产科学研究院黄海水产研究所 | Method for balancing fatty acid in fish feed, feed and application |
CN115500433B (en) * | 2022-09-27 | 2024-03-26 | 中国水产科学研究院黄海水产研究所 | Method for balancing fatty acid in fish feed, feed and application |
Also Published As
Publication number | Publication date |
---|---|
CN114847418B (en) | 2023-08-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102178086B (en) | Feed additive for improving quality and flavor of pork, feed, and preparation method thereof | |
CN111387373A (en) | Feed for juvenile Pacific carps with hermetia illucens larvae replacing fish meal and preparation method of feed | |
CN110367398A (en) | It is a kind of to help to improve fresh fish matter and the expanded pellet diet of flavor and preparation method thereof | |
CN107348122A (en) | A kind of raw fish tissue quality-improving special feed and its application | |
CN114847418A (en) | Composite fat source for improving muscle quality of tilapia based on accurate nutritional requirement of fatty acid | |
CN101326966A (en) | Feedstuff additive for improving quality of large yellow croaker | |
CN107912646A (en) | A kind of largemouth bass compound feed for young | |
CN104621406A (en) | Puffed pellet compound feed for adult alligator snapping turtles | |
CN105231098B (en) | A kind of additive and its preparation method and application improving meat quality of fish | |
Mesaa et al. | Time course of muscle fatty acid composition of cultured meagre (Argyrosomus regius) during the first sixteen months of a cage culture | |
Tandzong et al. | Effect of cassava leaf (Manihot esculenta) level in guinea-pigs (Cavia porcellus) meal on the physico-chemical and techno-logical properties of its meat | |
CN103300222B (en) | Application of rubber seedcake to oncorhynchus mykiss mixed feed and formula of feed | |
CN104621405A (en) | Puffed pellet compound feed for young alligator snapping turtles | |
CN104686836A (en) | Puffed granular compound feed for young alligator snapping turtle | |
CN103300256B (en) | Application of rubber seedcake to cyprinus carpio mixed feed and formula of feed | |
CN108065129A (en) | A kind of aquatic products compound premix and its application | |
CN114208969A (en) | Additive for improving quality of Sanhuang chicken and quality improving method | |
CN107927439A (en) | A kind of largemouth bass is into carp compound feed | |
CN112262795B (en) | Nutrition method for maintaining muscle fatty acid quality of low-fat type cultured fish | |
CN110476844B (en) | Method for improving and promoting fish muscle fiber development and DHA deposition | |
CN112753896A (en) | Low-fish-meal artificial compound feed for penaeus monodon, preparation method and application thereof | |
CN108522898A (en) | A kind of Pseudosciaena crocea meat modifier and preparation method thereof | |
Mohamed et al. | Body weight characteristics and chemical composition of Nile tilapia (Oreochromis niloticus) collected from three different Sudanese dams | |
Fall et al. | Effects of three varieties of melon seed meal on the growth performance, survival and whole body composition of Oreochromis niloticus fry | |
CN107484925A (en) | It is a kind of improve raw fish body color and liver health into carp compound feed |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |