CN114793792B - Method for restoring pollen fertility of different fertility populations of BNS series temperature-sensitive genic male sterile wheat - Google Patents
Method for restoring pollen fertility of different fertility populations of BNS series temperature-sensitive genic male sterile wheat Download PDFInfo
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- CN114793792B CN114793792B CN202210432677.7A CN202210432677A CN114793792B CN 114793792 B CN114793792 B CN 114793792B CN 202210432677 A CN202210432677 A CN 202210432677A CN 114793792 B CN114793792 B CN 114793792B
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
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Abstract
The invention discloses a method for restoring fertility of BNS series temperature-sensitive genic male sterile wheat pollen, which utilizes a MeJA solution with the concentration of 50-200 mu mol/L or a DIECA solution with the concentration of 5 mu mol/L-30mmol/L to carry out spraying treatment on BNS series temperature-sensitive genic male sterile wheat sterile population or a sterile population plant, and other conditions refer to a general field cultivation management mode. The invention utilizes the MeJA solution or the DIECA solution to spray and treat, can promote the pollen fertility recovery of different fertility groups of BNS series temperature-sensitive genic male sterile wheat, thereby reducing the seed production cost of hybrid wheat.
Description
Technical Field
The invention belongs to the technical field of chemical regulation and control of pollen fertility of different fertility groups of BNS (brain-associated bacteria) series temperature-sensitive genic male sterile wheat, and particularly relates to a method for restoring pollen fertility of different fertility groups of BNS series temperature-sensitive genic male sterile wheat, in particular to a chemical regulation and control method for promoting pollen fertility restoration of different fertility groups of BNS series temperature-sensitive genic male sterile wheat by using Methyl jasmonate (MeJA) solution or diethyl dithiocarbamate (sodium diethyldithiocarbamate, DIECA) solution.
Background
The use of wheat photo-thermo-sensitive male sterility (PTSMS) is a significant advance in research of hybrid wheat. The wheat photo-thermo-sensitive sterile lines found in China, such as ES, C49S, ZP, BS, YS, BS, BNS and the like, become important test materials for research of hybrid wheat.
The temperature sensitive genic male sterile wheat BNS is successfully cultivated in 2002 by Ru Zhengang, and a series of male sterile lines are cultivated by taking the BNS as a parent. BNS series temperature-sensitive male sterile line has the characteristics of thorough sterility and thorough fertility conversion, and the fertility change trend is as follows: complete abortion-semi-sterile-complete fertility. Low temperature is the main cause affecting fertility transition of BNS-series materials, exhibiting low temperature sterility and high temperature fertility. The sensitive part of BNS series material which senses low temperature is the young spike which develops. The fertility transition sensitive period of BNS series materials is 5-18d before heading, namely the differentiation period of male and female stamen is up to the four-split formation period, and the BNS series materials show complete sterility when the temperature is lower than 8 ℃ in the temperature sensitive period, so as to form a sterile line; 8-12 ℃ is fertility transition temperature; the plant shows complete fertility at the temperature higher than 12 ℃, and the formed fertility line is the maintainer line. Sowing in the North Hemsl region from 9 months 23 days to 10 months 17 days, and thoroughly sterility is shown, so that the method can be used for producing hybrid seeds; sowing after 11 months and 18 days shows complete fertility, and can selfing propagation of sterile line seeds. The BNS sterile line has the excellent characteristics of good sterility, easy conversion and specific recovery in the Huang-Huai wheat main production area of China, and is a sterile line material which has great utilization value and can be used for hybrid wheat. In the fertility transition sensitive period of BNS series materials, the minimum temperature periodic fluctuation is frequently generated, the fertility stability is also disturbed to be fluctuated, the fertility recovery difficulty problem of late-sowed fertile groups can be generated to reduce the yield, the cost and the risk of the seed production of two-line hybrid wheat are increased, and the BNS series materials are potentially severely threatened in large-area production application.
There are theoretically many possibilities for basic countermeasures against the interference of the periodic fluctuation of the lowest air temperature to the fertility stability of the BNS series of materials. One possibility is to combine the sensitivity of wheat to photoperiod, develop a double sensitive nuclear sterile line that is sensitive to both photoperiod and low temperature, but this requires a lengthy scientific research process to be possible, which is currently limited to theoretical analysis and is not possible in practice. Another possibility is to use modern agricultural facilities to promote short-term temperature rise, but this is a high facility requirement, which makes it difficult to meet the needs of large-area hybrid seed production. Under the condition that the fertility conversion sensitive period is influenced by the periodic fluctuation of the air temperature or the influence of the periodic fluctuation of the air temperature is predicted, the fertility is restored by being simultaneously assisted with proper chemical regulation measures, and the method can also be used as a remedial measure to improve the yield of the BNS series material late-seeding fertility population. In combination with temperature change, research for promoting great recovery of pollen fertility of BNS series materials by utilizing MeJA and DIECA at a specific development stage has not been reported at present.
Aiming at different fertility populations of the temperature sensitive genic male sterile wheat BNS series material, a novel chemical hybridization agent which has strong male fertility restoration effect and low price is screened, is used for promoting the fertility restoration of the BNS series material, and has important significance for effectively utilizing the BNS series material in hybrid wheat seed production and reducing the seed production cost.
Disclosure of Invention
The invention solves the technical problem of providing a method for restoring pollen fertility of different fertility groups of BNS series temperature-sensitive genic male sterile wheat, which can promote the restoration of pollen fertility of different fertility groups of BNS series temperature-sensitive genic male sterile wheat by spraying MeJA solution or DIECA solution, thereby reducing the seed production cost of hybrid wheat.
The invention adopts the following technical scheme for solving the technical problems: a method for restoring fertility of BNS series temperature-sensitive genic male sterile wheat pollen is characterized by comprising the following steps: in a Huang-Huai wheat main producing area, sowing BNS series temperature-sensitive genic male sterile wheat under the sterile sowing condition, namely 9 months 23 days to 10 months 17 days to form a BNS series temperature-sensitive genic male sterile wheat sterile group, and sowing BNS series temperature-sensitive genic male sterile wheat after 11 months 18 days to form a BNS series temperature-sensitive genic male sterile wheat sterile group;
aiming at BNS series temperature-sensitive genic male sterile wheat sterile population, the time from the differentiation period of the male and female primordia to the drug isolation period lasts for 5-8 days, and the daily average temperature is lower than 8 ℃; for 6-13d, the daily average temperature is lower than 10 ℃ before the medicine interval forming period to meiosis; spraying exogenous methyl jasmonate solution to BNS series temperature-sensitive genic male sterile wheat plants for 3-5d at a daily average temperature lower than 10 ℃ in the period from the pre-meiosis to tetrad, wherein the spraying concentration of the methyl jasmonate solution is 50-200 mu mol/L, and the spraying amount of the methyl jasmonate solution is 50-80L/mu;
aiming at the BNS series temperature-sensitive genic male sterile wheat fertility population, the time from the differentiation period of the male and female primordia to the drug isolation period lasts for 5-8 days, and the daily average temperature is higher than 10 ℃; during the period from the formation of medicine septum to the pre-meiosis, for 6-13d, the daily average temperature is higher than 14 ℃; and (3) carrying out spraying treatment on the exogenous diethyl dithiocarbamate solution on BNS series temperature-sensitive genic male sterile wheat plants after 3-5d and when the daily average temperature is higher than 14 ℃ in the period from the pre-meiosis to the tetrad, wherein the spraying concentration of the diethyl dithiocarbamate solution is 5 mu mol/L-30mmol/L, and the spraying amount of the diethyl dithiocarbamate solution is 50-80L/mu.
The two groups of reagents can be selected to be sprayed according to the temperature change along with the development process of the wheat, and similar effects can be obtained.
Further limited, the spraying time of the methyl jasmonate solution or the diethyl dithiocarbamate solution is 18:00-19:00 in the evening, and the spraying treatment is carried out every 3d from the occurrence of the proper treatment temperature condition corresponding to each spraying stage.
Further defined, the spraying concentration of the methyl jasmonate solution is 100 mu mol/L; the spraying concentration of the diethyl dithiocarbamic acid solution is 10mmol/L.
The invention adopts chemical drugs to spray the BNS series temperature-sensitive genic male sterile wheat plants, thus overcoming the defects of temperature control technology, more conveniently regulating and controlling BNS series materials, promoting pollen fertility recovery, reducing seed production cost of hybrid wheat and having less strict requirements on facilities. The method of the invention can eliminate the requirement of controlling temperature on facilities and manpower, and has less investment.
The invention selects MeJA solution with the concentration of 50-200 mu mol/L or DIECA solution with the concentration of 5 mu mol/L-30mmol/L to spray the BNS series temperature-sensitive genic male sterile wheat plants, and other conditions are referred to a common field cultivation management mode. Through field cultivation, the MeJA solution with the concentration of 50-200 mu mol/L or the DIECA solution with the concentration of 10 mu mol/L-30mmol/L is adopted for spraying treatment, so that the pollen fertility of different fertility groups of BNS series temperature-sensitive genic male sterile wheat is recovered, the seed production cost of hybrid wheat is reduced, and at present, the success is achieved through experiments. The invention has the advantages of relatively simple operation, lower cast cost, time and labor saving and wide application prospect.
Detailed Description
The above-described matters of the present invention will be described in further detail by way of examples, but it should not be construed that the scope of the above-described subject matter of the present invention is limited to the following examples, and all techniques realized based on the above-described matters of the present invention are within the scope of the present invention.
Example 1
And (3) taking the temperature sensitive genic male sterile wheat BNS366 as a test material, and sowing at two sowing periods of 10 months, 12 days and 12 months, 2 days in 2020 to form a BNS366 male sterile group and a male sterile group. The test site is a wheat test field of Henan university, the soil is sandy loam, and the cultivation and management are carried out according to a common field cultivation mode. Starting from the period from the development of the thermo-sensitive genic male sterile wheat BNS366 stamen to the differentiation of the male and female stamen primordium, stopping in the tetrad period, carrying out MeJA solution spraying treatment on BNS366 groups (normal autumn-sown sterile groups) formed by sowing on 10 and 12 days in 2020, and carrying out DIECA solution spraying treatment on BNS366 groups (late-sown fertile groups) formed by sowing on 12 and 2 days in 2020 according to weather forecast and actual air temperature monitoring conditions.
Between 7 and 10 days of 3 months of 2021, the daily average air temperatures are respectively: 3.5 ℃ (3 months and 7 days), 5.9 ℃ (3 months and 8 days), 9.2 ℃ (3 months and 9 days) and 8.1 ℃ (3 months and 10 days), the BNS366 normal autumn-sowing sterile population is in the stage of the differentiation phase-the medicine interval phase of the stamen primordium, and the air temperature is more below 8 ℃; between 16 days of 3 months and 22 days of 3 months, the daily average air temperatures are respectively: 8.3 ℃ (3 months and 18 days) and 8.0 ℃ (3 months and 19 days), 8.3 ℃ (3 months and 20 days), 9.6 ℃ (3 months and 21 days) and 9.2 ℃ (3 months and 22 days), BNS366 is in the stage of primordium differentiation phase-medicine interval phase of the gynoecial stamen and the temperature is below 10 ℃. Meeting the condition of inducing the pollen fertility to restore by the MeJA solution, setting 4 times of spraying treatment of 3 months, 7 days, 3 months, 10 days, 3 months, 19 days and 3 months, 22 days, and spraying the MeJA solution with the concentration of 100 mu mol/L.
Between day 23 and day 2 of 3 and 4 of 2021, the daily average air temperatures are respectively: 15.2 ℃ (3 months 25 days), 13.8 ℃ (3 months 26 days), 15.1 ℃ (3 months 27 days), 16.0 ℃ (3 months 28 days), 15.6 ℃ (3 months 29 days), 14.8 ℃ (3 months 30 days), 13.8 ℃ (3 months 31 days) and 14.5 ℃ (4 months 1 day), the BNS366 late-sown fertile population is in the medicine interval-tetrad period, the air temperature is higher than 14 ℃ and accords with the condition that the DIECA solution induces the pollen fertility recovery thereof, 3 times of spraying treatment are carried out for 3 months 25 days, 3 months 28 days and 3 months 31 days, and the DIECA solution with the concentration of 10mmol/L is sprayed.
Both treatments used the sprayed water as a Control (CK). The spraying amount is 50-80L/mu. The spraying time is 18:00-19:00 of evening. Repeated 3 times. The fertility rate and the setting rate (national and international) of the pollen were measured for each treatment.
Pollen fertility rate (%) = [ number of viable pollen/(number of viable pollen+number of sterile pollen) ]. Times.100%.
Setting rate (%) = (basal nodule real number/spike number×2) ×100% (domestic method);
set rate (%) = (number of grains per spike×2) ×100% (international method).
The fertility measurement results of the BNS366 sterile line are shown in Table 1.
TABLE 1 fertility comparison of temperature sensitive genic male sterile wheat BNS366 sterile populations and fertile populations under different treatment methods
Note that: post-data and post-data represent differences at the 0.01 and 0.05 levels for each treatment of the same column numbers, respectively, compared to the corresponding control.
Experiments prove that: referring to a general field cultivation management mode, spraying MeJA solution with the treatment concentration of 100 mu mol/L can promote the fertility restoration of the sterile group pollen of the temperature sensitive genic male sterile wheat BNS366, spraying DIECA solution with the treatment concentration of 10mmol/L can promote the fertility restoration of the sterile group pollen of the BNS366, and meanwhile, the saturated pollination seed setting rate is consistent with that of a control, so that the novel technology for promoting the fertility restoration of the sterile group pollen of the temperature sensitive genic male sterile wheat BNS366 can overcome the interference of temperature fluctuation on the fertility of the sterile group pollen, and the seed production cost of hybrid wheat is reduced.
The foregoing embodiments illustrate the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the scope of the principles of the invention, which are defined in the appended claims.
Claims (3)
1. A method for restoring pollen fertility of different fertility groups of BNS series temperature-sensitive genic male sterile wheat is characterized by comprising the following steps: in a Huang-Huai wheat main producing area, sowing BNS series temperature-sensitive genic male sterile wheat under the sterile sowing condition, namely 9 months 23 days to 10 months 17 days to form a BNS series temperature-sensitive genic male sterile wheat sterile group, and sowing BNS series temperature-sensitive genic male sterile wheat after 11 months 18 days to form a BNS series temperature-sensitive genic male sterile wheat sterile group;
aiming at BNS series temperature-sensitive genic male sterile wheat sterile population, the time from the differentiation period of the male and female primordia to the drug isolation period lasts for 5-8d, and the daily average temperature is lower than 8 ℃; during the period from the formation of medicine septum to the pre-meiosis, the daily average temperature is lower than 10 ℃ for 6-13 d; spraying exogenous methyl jasmonate solution to BNS series temperature-sensitive genic male sterile wheat plants at a daily average temperature lower than 10 ℃ in a period from the pre-meiosis period to the tetrad period for 3-5d, wherein the spraying concentration of the methyl jasmonate solution is 50-200 mu mol/L, and the spraying amount of the methyl jasmonate solution is 50-80L/mu;
aiming at the BNS series temperature-sensitive genic male sterile wheat fertility population, the time from the differentiation period of the male and female primordia to the drug isolation period lasts for 5-8d, and the daily average temperature is higher than 10 ℃; during the period from the formation of medicine septum to the pre-meiosis, the daily average temperature is higher than 14 ℃ for 6-13 d; and (3) spraying exogenous diethyl dithiocarbamic acid solution to BNS series temperature-sensitive genic male sterile wheat plants at a time of 3-5d days and at an average temperature higher than 14 ℃ in the period from the pre-meiosis to tetrad, wherein the spraying concentration of the diethyl dithiocarbamic acid solution is 5 mu mol/L-30mmol/L, and the spraying amount of the diethyl dithiocarbamic acid solution is 50-80L/mu.
2. The method for restoring pollen fertility to a different fertility population of BNS-series thermo-sensitive genic male sterile wheat of claim 1, wherein: the spraying time of the methyl jasmonate solution or the diethyl dithiocarbamic acid solution is 18:00-19:00 in the evening, and the spraying treatment is carried out every 3d from the occurrence of the proper treatment temperature condition corresponding to each spraying stage.
3. The method for restoring pollen fertility to a different fertility population of BNS-series thermo-sensitive genic male sterile wheat of claim 1, wherein: the spraying concentration of the methyl jasmonate solution is 100 mu mol/L; the spraying concentration of the diethyl dithiocarbamic acid solution is 10mmol/L.
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| CN1196655A (en) * | 1995-09-21 | 1998-10-21 | 华盛顿州立大学研究基金会 | Conditionally male-fertile plants and methods and compositions for restoring fertility thereof |
| WO2002052926A2 (en) * | 2001-01-05 | 2002-07-11 | Northwest Plant Breeding Co. | Methods for generating doubled haploid maize plants |
| CN106070215A (en) * | 2016-06-20 | 2016-11-09 | 扬州大学 | A kind of photo-thermo sensitive male nuclear sterile rice line opens clever accelerator and compound method thereof and application under high temperature stress |
| CN110129361A (en) * | 2019-06-03 | 2019-08-16 | 清华大学 | The method of creating plants male sterility system |
| CN110226472A (en) * | 2019-07-04 | 2019-09-13 | 新疆农垦科学院 | It is a kind of to remove the propagation method for keeping the wheat cytoplasm male sterile line seed of system from |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0307791B1 (en) * | 2002-02-20 | 2020-08-11 | J.R. Simplot Company | METHODS TO MODIFY CHARACTERISTICS OF SELECTED PLANT |
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Patent Citations (5)
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| CN1196655A (en) * | 1995-09-21 | 1998-10-21 | 华盛顿州立大学研究基金会 | Conditionally male-fertile plants and methods and compositions for restoring fertility thereof |
| WO2002052926A2 (en) * | 2001-01-05 | 2002-07-11 | Northwest Plant Breeding Co. | Methods for generating doubled haploid maize plants |
| CN106070215A (en) * | 2016-06-20 | 2016-11-09 | 扬州大学 | A kind of photo-thermo sensitive male nuclear sterile rice line opens clever accelerator and compound method thereof and application under high temperature stress |
| CN110129361A (en) * | 2019-06-03 | 2019-08-16 | 清华大学 | The method of creating plants male sterility system |
| CN110226472A (en) * | 2019-07-04 | 2019-09-13 | 新疆农垦科学院 | It is a kind of to remove the propagation method for keeping the wheat cytoplasm male sterile line seed of system from |
Non-Patent Citations (2)
| Title |
|---|
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| 茉莉酸类与光敏核不育水稻N5088S育性的关系;姚锋先等;江西农业大学学报;第29卷(第1期);第6-10页 * |
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