CN114686338A - Eccentric vibration platform for cell transfection - Google Patents
Eccentric vibration platform for cell transfection Download PDFInfo
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- CN114686338A CN114686338A CN202210425406.9A CN202210425406A CN114686338A CN 114686338 A CN114686338 A CN 114686338A CN 202210425406 A CN202210425406 A CN 202210425406A CN 114686338 A CN114686338 A CN 114686338A
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- 238000001890 transfection Methods 0.000 title claims abstract description 46
- 230000007246 mechanism Effects 0.000 claims abstract description 54
- 230000005540 biological transmission Effects 0.000 claims abstract description 49
- 230000033001 locomotion Effects 0.000 claims abstract description 10
- 238000003151 transfection method Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 33
- 238000000034 method Methods 0.000 description 9
- 230000008878 coupling Effects 0.000 description 6
- 238000010168 coupling process Methods 0.000 description 6
- 238000005859 coupling reaction Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000010362 genome editing Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- Biomedical Technology (AREA)
- Sustainable Development (AREA)
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- Biochemistry (AREA)
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Abstract
The invention relates to the technical field of cell transfection equipment, and aims to provide an eccentric vibration platform for cell transfection, which has high stability and low cost and is suitable for a novel microneedle-vibration transfection method. The eccentric vibration platform for cell transfection comprises a support, a bevel gear eccentric power mechanism, a vibration mechanism and a sliding mechanism, wherein a transmission shaft of the bevel gear eccentric power mechanism is erected on the support, eccentric rods are arranged at two ends of the transmission shaft, the eccentric rods are fixedly connected with the lower end of the vibration mechanism, and the upper end of the vibration mechanism is suitable for reciprocating motion on the sliding mechanism along the vertical direction. The invention solves the problems of higher cost and poorer vibration stability of the existing vibration device.
Description
Technical Field
The invention relates to the technical field of cell transfection equipment, in particular to an eccentric vibration platform for cell transfection.
Background
Cell transfection refers to a process of introducing an exogenous nucleic acid fragment into a cell, and has great use value in the fields of gene editing, immunotherapy, regenerative medicine, basic biology and the like. With the development of molecular and cell biology research, transfection has become a common tool for studying and controlling gene function in eukaryotic cells. In biological tests such as research of gene function, regulation of gene expression, mutation analysis, and protein production, the application of the method is more and more extensive.
The traditional cell transfection methods include electroporation, photothermal methods, fluid shearing, microinjection, liposome methods, viral methods, chemical methods and the like, but most of the methods stay in the laboratory stage, can only complete cell transfection under the conditions of small scale and high cost, and have insufficient stability and safety, and have poor effect on key cells (such as stem cells, immune cells and the like) required by current scientific research and engineering applications. The existing vibrating device has a relatively complex structure and a large integral volume, so that the cost is high, the vibration stability is poor, the using condition of cell transfection cannot be met, and the efficiency of a cell transfection experiment is influenced.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is to overcome the defects of high cost and poor vibration stability of the vibration device in the prior art, so as to provide an eccentric vibration platform for cell transfection, which has high vibration stability and low cost.
In order to solve the problems, the invention provides an eccentric vibration platform for cell transfection, which comprises a support, a bevel gear eccentric power mechanism, a vibration mechanism and a sliding mechanism, wherein a transmission shaft of the bevel gear eccentric power mechanism is erected on the support, eccentric rods are arranged at two ends of the transmission shaft, the eccentric rods are fixedly connected with the lower end of the vibration mechanism, and the upper end of the vibration mechanism is suitable for reciprocating motion on the sliding mechanism along the vertical direction.
Preferably, the bevel gear eccentric power mechanism comprises a power motor, a first bevel gear and a second bevel gear, the first bevel gear is coaxially and fixedly connected with an output shaft of the power motor, the second bevel gear is coaxially and fixedly connected with the transmission shaft, the first bevel gear and the second bevel gear are mutually vertical in axial direction, and the first bevel gear is meshed with the second bevel gear.
Preferably, a first rotating bearing is arranged between the transmission shaft and the bracket.
Preferably, the first bevel gear is fixedly connected with an output shaft of the power motor through an adapter plate.
Preferably, the vibration mechanism comprises a transmission rod and a fixed plate, the lower end of the transmission rod is fixedly connected with the eccentric rod, the upper end of the transmission rod is rotatably connected with the fixed plate, and the fixed plate is suitable for reciprocating on the sliding mechanism along the vertical direction.
Preferably, the vibration mechanism further comprises a vibration plate fixedly connected to an upper portion of the fixing plate.
Preferably, the fixing plate includes a vertical portion and a horizontal portion, the vertical portion is provided with a connecting short shaft and a slider connecting hole, and the horizontal portion is formed with a vibration plate connecting hole.
Preferably, both ends of the transmission rod are provided with connecting holes, and a second rotating bearing is arranged in the connecting hole of the transmission rod matched with the fixed plate.
Preferably, the sliding mechanism comprises a sliding rail and a sliding block, the sliding block is connected with the sliding rail in a sliding manner, the sliding block is fixedly connected with the fixing plate, and the sliding rail is relatively fixed with the bracket.
Preferably, still include the chassis, support and slide rail all set firmly on the chassis, and the chassis bottom is equipped with two at least bases.
The invention has the following advantages:
1. according to the eccentric vibration platform for cell transfection, the bevel gear eccentric power mechanism erected on the support can drive the vibration mechanism to reciprocate on the sliding mechanism in the vertical direction, so that stable kinetic energy required by cell transfection is provided, meanwhile, the structure of the device is simplified, the overall size of the device is reduced, the manufacturing cost is saved, the vibration platform suitable for a brand-new microneedle vibration transfection method is provided, the motion frequency and the acceleration of the vibration disk can be changed by adjusting the rotating speed of the power motor, and accurate control of cell transfection parameters is realized.
2. The eccentric vibration platform for cell transfection provided by the invention adopts a mode of meshing transmission of the two bevel gears, compared with other types of gears, the bevel gears have better rotation stability, smaller rotation noise and larger rotation torque, and simultaneously, the directions of the power motor and the vibration can be adjusted according to actual use requirements.
3. According to the eccentric vibration platform for cell transfection, the bearings are arranged between the transmission shaft and the bracket and between the transmission rod and the fixed plate, so that the platform can vibrate more smoothly.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram showing the structure of an eccentric vibration platform for cell transfection according to the present invention;
FIG. 2 is a front view of the eccentric vibrating platform for cell transfection of the present invention;
FIG. 3 shows a schematic view of the driving shaft of the eccentric vibration platform for cell transfection according to the present invention;
FIG. 4 shows a schematic view of the driving rod in the eccentric vibrating platform for cell transfection according to the present invention;
FIG. 5 is a schematic diagram showing the fixed plate of the eccentric vibration platform for cell transfection according to the present invention.
Description of reference numerals: 1. a chassis; 2. a power motor; 3. a first bevel gear; 4. a second bevel gear; 5. a drive shaft; 501. an eccentric rod; 6. a support; 7. a transmission rod; 701. connecting holes; 8. a fixing plate; 801. connecting the short shaft; 802. a horizontal portion; 803. a slider connecting hole; 804. a vertical portion; 805. a vibration plate connection hole; 9. a slider; 10. a slide rail; 11. a vibrating plate; 12. a base; 13. an adapter plate.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example one
As shown in FIGS. 1 and 2, the preferred embodiment of the eccentric vibration platform for cell transfection is shown in the present invention.
In this embodiment, the eccentric vibration platform for cell transfection includes a support 6, a bevel gear eccentric power mechanism, a vibration mechanism and a sliding mechanism, the bevel gear eccentric power mechanism is fixedly connected with the lower end of the vibration mechanism, the bevel gear eccentric power mechanism drives the vibration mechanism to reciprocate on the sliding mechanism along the vertical direction, so that the vibration of the vibration mechanism in the vertical direction is realized, stable kinetic energy required by cell transfection is provided, the structure of the device is simplified, the overall size of the device is reduced, and the manufacturing cost is saved.
The eccentric vibration platform of the embodiment further comprises a chassis 1, and at least two bases 12 are further arranged at the bottom of the chassis 1. In the present embodiment, four pedestals 12 are provided at the bottom of the chassis 1. The bracket 6 and the sliding mechanism are both fixed on the chassis 1. The chassis 1, the base 12 and the bracket 6 together form a bearing foundation of the eccentric vibration platform, and the base 12 is suitable for being placed on the ground.
The bevel gear eccentric power mechanism comprises a power motor 2, a first bevel gear 3, a second bevel gear 4 and a transmission shaft 5, wherein the power motor 2 is fixed on the chassis 1, an output shaft of the power motor 2 is arranged in the vertical direction, the first bevel gear 3 is fixed on the output shaft of the power motor 2, the first bevel gear 3 and the output shaft of the power motor 2 are arranged coaxially, the second bevel gear 4 is sleeved on the transmission shaft 5, the second bevel gear 4 is fixed on the transmission shaft 5 coaxially, the first bevel gear 3 and the second bevel gear 4 are arranged in a mutually perpendicular mode in the axial direction, and the first bevel gear 3 and the second bevel gear 4 are in meshing transmission. In this embodiment, the first bevel gear 3 is fixed on the output shaft of the power motor 2 through the adapter plate 13, so the first bevel gear 3 is also arranged in the vertical direction, the second bevel gear 4 is arranged in the horizontal direction, the second bevel gear 4 is engaged with the first bevel gear 3, and the teeth of the first bevel gear 3 and the second bevel gear 4 are both arranged at 45 degrees. Through the mode of two bevel gears meshing transmission, can adjust the direction of power motor 2 and vibration mechanism according to the in-service use demand.
In this embodiment, the transmission shaft 5 is mounted on the bracket 6, and the transmission shaft 5 is in supporting connection with the bracket 6 through the first rotating bearing, so that the transmission shaft 5 rotates more smoothly.
In this embodiment, as shown in fig. 3, the two ends of the transmission shaft 5 are provided with eccentric rods 501, the eccentric rods 501 are fixed at the lower end of the vibration mechanism, the transmission shaft 5 is driven by the rotation of the second bevel gear 4, and the eccentric rods 501 are used for providing a power source for the vibration mechanism.
In this embodiment, the vibrating mechanism includes a transmission rod 7, a fixing plate 8 and a vibrating plate 11, the lower end of the transmission rod 7 is fixedly connected with the eccentric rod 501, the upper end of the transmission rod 7 is rotatably connected with the fixing plate 8, the vibrating plate 11 is fixed on the upper portion of the fixing plate 8, the vibrating plate 11 is suitable for placing a microfluidic chip integrated with a nanoneedle array, and the cell suspension and the substance to be transfected are introduced into a micro channel of the chip together from an inlet, so as to realize high-throughput cell transfection with low cytotoxicity. The fixed plate 8 reciprocates in the vertical direction on the slide mechanism, thereby driving the vibration plate 11 to vibrate in the vertical direction.
As shown in fig. 4 and 5, both ends of the driving lever 7 have coupling holes 701, the fixing plate 8 includes a vertical portion 804 and a horizontal portion 802, the vertical portion 804 has a coupling stub 801 and a slider coupling hole 803, and the horizontal portion 802 has a vibration plate coupling hole 805. In this embodiment, the connection hole 701 of the lower end of the transmission rod 7 is fixed on the eccentric rod 501, the connection hole 701 of the upper end of the transmission rod 7 is provided with a second rotation bearing, and the connection hole 701 of the upper end of the transmission rod 7 is rotatably connected with the connection stub 801 of the fixing plate 8, the second rotation bearing is provided to make the movement of the fixing plate 8 smoother, the fixing plate 8 is fixedly connected with the vibration plate 11 through the vibration plate connection hole 805, and the fixing plate 8 is fixed on the sliding mechanism through the slider connection hole 803. In the present embodiment, the number of the vibration plate connection holes 805 is four, and the vibration plate connection holes 805 are fitted to the vibration plate 11 with a coupling member such as a bolt, a nut, etc., and the number of the slider connection holes 803 is four, and the slider connection holes 803 are fitted to the sliding mechanism with a coupling member such as a bolt, a nut, etc.
In the embodiment, the middle part of the transmission rod 7 is provided with a rectangular through hole structure, so that the weight of the transmission rod 7 can be reduced, and the manual installation of the transmission rod 7 is facilitated.
In this embodiment, the sliding mechanism includes a sliding rail 10 and a sliding block 9, the sliding block 9 is slidably connected to the sliding rail 10, the sliding rail 10 is fixed on the chassis 1, so that the sliding rail 10 is fixed relative to the bracket 6, and the sliding block 9 is fixedly connected to the fixing plate 8 through a sliding block connecting hole 803.
In the present embodiment, the slide rail 10 is disposed outside the bracket 6, and both the slide rail 10 and the bracket 6 are L-shaped.
The working procedure of the eccentric vibration platform for cell transfection in this example is described below:
firstly, placing a micro-fluidic chip which is introduced with cell suspension and integrated with a nano-needle array with transfection substances on the top of a vibrating plate 11;
the power motor 2 is started, the first bevel gear 3 is driven to rotate through the adapter plate 13, so as to drive the second bevel gear 4 meshed with the first bevel gear 3 to rotate, and further drive the transmission shaft 5 fixedly connected with the second bevel gear 4 to rotate, the eccentric rods 501 at two ends of the transmission shaft 5 perform circular motion around the axis of the transmission shaft 5, so as to drive the lower end of the transmission rod 7 to perform circular motion, because the upper end of the transmission rod 7 is connected with the fixed plate 8, the fixed plate 8 is fixed on the slide block 9, the slide block 9 can only perform vertical sliding on the slide rail 10, so that the slide block 9 and the slide rail 10 limit the horizontal motion of the fixed plate 8, the upper end of the transmission rod 7 drives the fixed plate 8 to perform vertical reciprocating motion on the slide rail 10, and finally drives the vibration plate 11 fixed on the fixed plate 8 to perform vertical reciprocating motion, so as to realize the vibration of the vibration plate 11, and finally drive the micro-fluidic chip placed on the vibration plate 11 to vibrate therewith, thereby completing the process of cell transfection. The transfection flux of cell transfection can be increased through the device, and the working efficiency of cell transfection is improved.
Example two
The eccentric vibration platform for cell transfection of this example differs from that of the first example in that: in order to simplify the structure and save the cost, the adapter plate 13 can be eliminated under the condition that the length of the power motor 2 meets the requirement.
EXAMPLE III
The eccentric shaking platform for cell transfection of this example differs from that of the first example in that: in order to simplify the structure and save the cost, the base 12 can be eliminated under the condition that the whole height of the device meets the requirement.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications therefrom are within the scope of the invention.
Claims (10)
1. An eccentric vibrating platform for cell transfection, characterized by: the bevel gear eccentric power mechanism comprises a support (6), a bevel gear eccentric power mechanism, a vibration mechanism and a sliding mechanism, wherein a transmission shaft (5) of the bevel gear eccentric power mechanism is erected on the support (6), eccentric rods (501) are arranged at two ends of the transmission shaft (5), the eccentric rods (501) are fixedly connected with the lower end of the vibration mechanism, and the upper end of the vibration mechanism is suitable for reciprocating motion in the vertical direction on the sliding mechanism.
2. The eccentric vibratory platform for cell transfection of claim 1 wherein: the bevel gear eccentric power mechanism comprises a power motor (2), a first bevel gear (3) and a second bevel gear (4), the first bevel gear (3) is coaxially and fixedly connected with an output shaft of the power motor (2), the second bevel gear (4) is coaxially and fixedly connected with the transmission shaft (5), the first bevel gear (3) and the second bevel gear (4) are perpendicular to each other in the axial direction, and the first bevel gear (3) is meshed with the second bevel gear (4).
3. The eccentric vibratory platform for cell transfection of claim 2 wherein: and a first rotating bearing is arranged between the transmission shaft (5) and the bracket (6).
4. The eccentric vibratory platform for cell transfection of claim 2 wherein: the first bevel gear (3) is fixedly connected with an output shaft of the power motor (2) through an adapter plate (13).
5. The eccentric vibratory platform for cell transfection of claim 1 wherein: the vibrating mechanism comprises a transmission rod (7) and a fixing plate (8), the lower end of the transmission rod (7) is fixedly connected with the eccentric rod (501), the upper end of the transmission rod (7) is rotatably connected with the fixing plate (8), and the fixing plate (8) is suitable for reciprocating on the sliding mechanism in the vertical direction.
6. The eccentric vibratory platform for cell transfection of claim 5 wherein: the vibration mechanism further comprises a vibration plate (11), and the vibration plate (11) is fixedly connected with the upper part of the fixing plate (8).
7. The eccentric vibratory platform for cell transfection of claim 6 wherein: the fixing plate (8) comprises a vertical part (804) and a horizontal part (802), wherein a connecting short shaft (801) and a sliding block connecting hole (803) are arranged on the vertical part (804), and a vibration plate connecting hole (805) is formed in the horizontal part (802).
8. The eccentric vibratory platform for cell transfection of claim 7 wherein: both ends of the transmission rod (7) are provided with connecting holes (701), and the transmission rod (7) is matched with the fixing plate (8) and a second rotating bearing is arranged in the connecting holes (701).
9. The eccentric vibratory platform for cell transfection of claim 5 wherein: the sliding mechanism comprises a sliding rail (10) and a sliding block (9), the sliding block (9) is in sliding connection with the sliding rail (10), the sliding block (9) is fixedly connected with the fixing plate (8), and the sliding rail (10) is relatively fixed with the support (6).
10. The eccentric vibratory platform for cell transfection of claim 9 wherein: the support is characterized by further comprising a base plate (1), the support (6) and the sliding rails (10) are fixedly arranged on the base plate (1), and at least two bases (12) are arranged at the bottom of the base plate (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210425406.9A CN114686338A (en) | 2022-04-21 | 2022-04-21 | Eccentric vibration platform for cell transfection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210425406.9A CN114686338A (en) | 2022-04-21 | 2022-04-21 | Eccentric vibration platform for cell transfection |
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| CN202210425406.9A Pending CN114686338A (en) | 2022-04-21 | 2022-04-21 | Eccentric vibration platform for cell transfection |
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|---|---|---|---|---|
| CN200967043Y (en) * | 2006-10-09 | 2007-10-31 | 王海清 | Multi-point vibrator |
| US20100234197A1 (en) * | 2009-03-11 | 2010-09-16 | Jason Huang | Oscillation platform |
| US20120279739A1 (en) * | 2010-02-26 | 2012-11-08 | Omron Healthcare Co., Ltd. | Fixed structure of an eccentric rod, and vibration generator |
| CN103013827A (en) * | 2013-01-18 | 2013-04-03 | 辽宁医学院 | Cell vibration meter |
| CN103752492A (en) * | 2013-11-22 | 2014-04-30 | 重庆盾铭电磁阀有限公司 | Up-and-down vibrating mechanism |
| CN208215597U (en) * | 2017-12-31 | 2018-12-11 | 高唐县鼎力建筑机械有限公司 | Concrete high frequency vibrator is set at a kind of staged horizontal bottom |
| CN113136333A (en) * | 2021-05-12 | 2021-07-20 | 清华大学 | Cell transfection system and method |
-
2022
- 2022-04-21 CN CN202210425406.9A patent/CN114686338A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200967043Y (en) * | 2006-10-09 | 2007-10-31 | 王海清 | Multi-point vibrator |
| US20100234197A1 (en) * | 2009-03-11 | 2010-09-16 | Jason Huang | Oscillation platform |
| US20120279739A1 (en) * | 2010-02-26 | 2012-11-08 | Omron Healthcare Co., Ltd. | Fixed structure of an eccentric rod, and vibration generator |
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| Title |
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Application publication date: 20220701 |
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