CN114570535B - Centrifugal device for umbilical cord mesenchymal stem cell separation - Google Patents
Centrifugal device for umbilical cord mesenchymal stem cell separation Download PDFInfo
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- CN114570535B CN114570535B CN202210489571.0A CN202210489571A CN114570535B CN 114570535 B CN114570535 B CN 114570535B CN 202210489571 A CN202210489571 A CN 202210489571A CN 114570535 B CN114570535 B CN 114570535B
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- fixing box
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- rotating shaft
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- 210000002901 mesenchymal stem cell Anatomy 0.000 title claims abstract description 19
- 210000003954 umbilical cord Anatomy 0.000 title claims abstract description 19
- 238000000926 separation method Methods 0.000 title claims description 8
- 230000007246 mechanism Effects 0.000 claims abstract description 38
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 13
- 238000007664 blowing Methods 0.000 claims abstract description 8
- 238000005119 centrifugation Methods 0.000 claims description 11
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 7
- 238000001125 extrusion Methods 0.000 description 6
- 210000000130 stem cell Anatomy 0.000 description 6
- 239000007787 solid Substances 0.000 description 5
- 241000883990 Flabellum Species 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 235000019580 granularity Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/02—Other accessories for centrifuges for cooling, heating, or heat insulating
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention discloses a centrifugal device for separating umbilical cord mesenchymal stem cells, which belongs to the technical field of centrifuges and comprises a fixing box, wherein a centrifugal mechanism is arranged in the fixing box, an air suction and blowing mechanism is arranged on the upper side of the fixing box, the left side and the right side of the lower surface of the fixing box are fixedly connected with a base through supporting columns, and the outer side of the fixing box is fixedly connected with a stabilizing mechanism. According to the test tube placing device, the centrifugal mechanism is arranged, and the test tubes can be placed into the placing holes, so that the test tubes can be centrifuged at the same time during centrifugal operation, when the same batch of test tubes are centrifuged and need to be taken out, the pressing rod is controlled to move downwards, people can take the placing plate out conveniently and take out all centrifuged test tubes at the same time, then people can place another placing plate prepared in advance into the two grooves and install the placing plate, and the other placing plate is filled with a plurality of test tubes to be centrifuged, so that the test tube disassembling and installing efficiency is greatly improved, and the test tube placing device is convenient for people to use.
Description
Technical Field
The invention belongs to the technical field of centrifuges, and particularly relates to a centrifuging device for separating umbilical cord mesenchymal stem cells.
Background
Centrifuges are machines that utilize centrifugal force to separate components of a mixture of liquid and solid particles or liquid and liquid. The centrifuge is mainly used for separating solid particles from liquid in suspension, or separating two liquids which have different densities and are insoluble with each other in emulsion (for example, cream is separated from milk); it can also be used to remove liquids from wet solids, such as by spin drying clothes in a washing machine; the special overspeed tubular separator can also separate gas mixtures with different densities; some settling centrifuges can also grade solid particles according to the density or the granularity by utilizing the characteristic that the solid particles with different densities or granularities have different settling speeds in liquid.
Stem cell centrifuge among the prior art generally utilizes the test-tube rack to fix a plurality of test tubes, separates the stem cell in a plurality of test tubes at the during operation, but the test tube is fixed comparatively troublesome, need take out the test tube one by one after the centrifugation finishes and refill new test tube that is equipped with stem cell, the troublesome poeration relatively influences processing speed, and centrifugal mechanism itself can produce from shaking in the course of the work simultaneously, leads to organism stability relatively poor, also can influence centrifugal work.
Disclosure of Invention
The invention aims to: in order to solve the stem cell centrifuge among the prior art and generally utilize the test-tube rack to fix a plurality of test tubes, separate the stem cell in a plurality of test tubes at the during operation, but the test tube is fixed comparatively troublesome, need take out the test tube one by one after the centrifugation finishes and refill the new test tube that is equipped with stem cell, the troublesome poeration relatively influences the processing speed, centrifugal mechanism can produce the self-shaking in the course of the work itself simultaneously, lead to the organism stability relatively poor, also may influence centrifugal operation's problem, and the centrifugal device is used in separation of umbilical cord mesenchymal stem cell who provides.
In order to achieve the purpose, the invention adopts the following technical scheme:
a centrifugal device for separating umbilical cord mesenchymal stem cells comprises a fixing box, wherein a centrifugal mechanism is arranged in the fixing box, an air suction and blowing mechanism is arranged on the upper side of the fixing box, the left side and the right side of the lower surface of the fixing box are fixedly connected with a base through a supporting column, the outer side of the fixing box is fixedly connected with a stabilizing mechanism, the stabilizing mechanism is sleeved on the outer side of the supporting column, a motor is fixedly connected with the inner part of the base, an output shaft of the motor penetrates through the upper surface of the base and is fixedly connected with a first rotating shaft, the top end of the first rotating shaft and the lower surface of the centrifugal mechanism, and the first rotating shaft is sleeved in a first bearing clamped on the lower surface of the fixing box;
the centrifugal mechanism comprises a U-shaped plate, a plurality of balls are fixedly connected to the outer surface of the U-shaped plate, the balls are slidably connected into an annular groove formed in the inner wall of the fixing box, the lower surface of the U-shaped plate is fixedly connected with the top end of the first rotating shaft, grooves are formed in the left side surface and the right side surface of the U-shaped plate, U-shaped cavities are formed in the left side and the right side of the inner part of the U-shaped plate, U-shaped rods are arranged in the U-shaped cavities, third springs are fixedly connected to the front side and the rear side of the lower surface of each U-shaped rod, first triangular blocks are fixedly connected to the front side and the rear side of the upper surface of each U-shaped rod, second triangular blocks are attached to the inclined surfaces of the two first triangular blocks, a first sliding rod is fixedly connected to the opposite surfaces of the two second triangular blocks, a first sliding sleeve is sleeved on the outer side of the first sliding sleeve, two ends of the first spring are fixedly connected with the opposite surfaces of the first sliding sleeve and the second triangular blocks respectively, the appearance chamber has been seted up at the top of U-shaped plate, holds the intracavity and is provided with according to the depression bar, presses the one end of depression bar and the back fixed connection of U-shaped bar, presses the upside that the other end of depression bar extended to the U-shaped plate.
As a further description of the above technical solution:
the lower surface of the inner wall of the groove is fixedly connected with a supporting plate through a second spring, and the outer surface of the supporting plate is attached to the inner wall of the groove.
As a further description of the above technical solution:
the placing plate is placed in the groove, a plurality of placing holes are formed in the placing plate, an inclined groove is formed in the position, corresponding to one end of the first sliding rod, of the lower surface of the placing plate, a second clamping groove is formed in the position, corresponding to the inclined groove, of the side face of the placing plate, and the second clamping groove is located on the upper side of the inclined groove.
As a further description of the above technical solution:
the air suction and blowing mechanism comprises a cover plate arranged on the fixed box, a second bearing is connected to the upper surface of the cover plate in a clamping mode, a second rotating shaft is sleeved in the second bearing, a bottom fixedly connected with base plate of the second rotating shaft, clamping blocks are fixedly connected to four corners of the lower surface of the base plate, and the clamping blocks are clamped into first clamping grooves formed in the upper surface of the placing plate.
As a further description of the above technical solution:
the outer fixed surface of second pivot is connected with the flabellum, and the flabellum is located the housing, and housing fixed connection has evenly seted up a plurality of suction holes at the upper surface of apron, and the upper surface intercommunication of housing has the air duct, and the air duct is the three-way pipe, and the other both ends of air duct all run through to the base inside and communicate and have the gas storage shell, and gas storage shell fixed connection is on the inner wall of base, and a plurality of fumaroles have been seted up to the surface of base.
As a further description of the above technical solution:
stabilizing mean includes the second slide bar of fixed connection in the fixed box left and right sides, and the outside cover of second slide bar is equipped with the second sliding sleeve, and the outside joint of second sliding sleeve has first push pedal, and a side that fixed box was kept away from to first push pedal is through two bracing piece fixedly connected with stripper plates.
As a further description of the above technical solution:
the lower fixed surface of first push pedal is connected with first articulated piece, and articulated on the first articulated piece have a connecting rod, and the other end of connecting rod articulates there is the articulated piece of second, and the articulated piece fixed connection of second is at the upper surface of lifting the board, and the equal joint in the left and right sides of lifting board upper surface has the stop collar, and the stop collar cover is established in the outside of pillar, lifts the upper surface of board and has seted up the through-hole, and first pivot is located the through-hole.
As a further description of the above technical solution:
the equal fixedly connected with third slide bar in the left and right sides at the lift board back, the outside cover of third slide bar is equipped with the third sliding sleeve, the third sliding sleeve joint is in the front of second push pedal, the outside cover of third slide bar is equipped with the fourth spring, the both ends of fourth spring respectively with the back of third sliding sleeve and the fixed surface connection of third slide bar, the back fixedly connected with handle of second push pedal, the equal fixedly connected with kelly in the positive left and right sides of second push pedal, the kelly card is gone into to the locating hole of seting up to the pillar surface, the quantity of locating hole is a plurality of, a plurality of locating holes are from last down align to grid setting.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. in the invention, by arranging the centrifugal mechanism, a plurality of test tubes can be placed in a plurality of placing holes, so that the plurality of test tubes can be simultaneously centrifuged during centrifugal operation, when the same batch of test tubes are centrifuged and need to be taken out, the pressing rod is controlled to move downwards, the pressing rod drives the U-shaped rod to move downwards, the U-shaped rod can drive the first triangular block to move downwards, the first triangular block cannot extrude the second triangular block, meanwhile, the first spring can push the second triangular block to move through self elastic extension, the second triangular block can drive the first sliding rod to move, one end of the first sliding rod is separated from the second clamping groove, meanwhile, the second spring can push the supporting plate to move upwards through self elastic extension, the supporting plate can push the placing plate from the grooves, people can conveniently take out the placing plate and simultaneously take out all the centrifuged test tubes, then, people place another placing plate prepared in advance into the two grooves and install the placing plate, a plurality of test tubes to be centrifuged have been filled on another placing plate, very big improvement the efficiency that the test tube was dismantled and was installed, made things convenient for people's use.
2. According to the invention, the inclined groove is arranged, the placing plate is placed in the groove and then pressed downwards, one end of the first sliding rod is positioned in the inclined groove under the action of the inclined groove, the two first sliding rods can be extruded and kept away from each other when the placing plate moves downwards, meanwhile, when the placing plate continues to move downwards, when the second clamping groove moves to the first sliding rod, the third spring can push the U-shaped rod to move upwards through the elastic extension of the third spring, at the moment, the first triangular block extrudes the second triangular block, meanwhile, the second triangular block moves and brings one end of the first sliding rod into the second clamping groove, the placing plate is well fixed, and the operation is very convenient.
3. According to the test tube cooling device, the air suction and blowing mechanism is arranged, the clamping blocks are clamped into the first clamping grooves, when the U-shaped plate and the placing plate rotate together, the placing plate can drive the base plate to rotate, meanwhile, the base plate can press the test tube to stably fix the test tube, the base plate can drive the fan blades to rotate through the second rotating shaft when rotating, the fan blades can suck air when rotating, the air can enter the housing through the air suction holes, then the air in the housing can enter the two air storage shells through the air guide tubes, the air in the air storage shells can be sprayed out through the air spraying holes, the purpose of cooling the motor is achieved, and the problem that the motor cannot effectively dissipate heat to affect work due to long-time work is avoided.
4. In the invention, by arranging the stabilizing mechanism, when the stability of the fixing box needs to be kept, the lifting plate is controlled to move upwards, the lifting plate can drive one ends of the two connecting rods to move upwards, the other ends of the connecting rods can drive the first push plate to move, the first push plate can drive the extrusion plates to move through the supporting rods, the two extrusion plates can be respectively attached to two external objects, the shaking degree of the fixing box is effectively reduced, the stability of the fixing box is kept, wherein the fourth spring can push up through self elasticity extension and move the second push pedal and remove, and the second push pedal can drive the kelly card and go into to the locating hole, and the position that lifts the board this moment can be fixed, sets up a plurality of locating holes, and the kelly can go into to the locating hole of difference, and two stripper plates can remove respectively to different position departments this moment, and the external object of different distance departments is lived in the laminating, reaches the purpose of fixed box according to the environment needs.
Drawings
Fig. 1 is a schematic structural diagram of a centrifugation device for umbilical cord mesenchymal stem cell separation according to the present invention;
fig. 2 is a schematic cross-sectional view of a U-shaped plate in a centrifugal device for umbilical cord mesenchymal stem cell isolation according to the present invention;
FIG. 3 is an enlarged schematic structural view of A in a centrifugal apparatus for separating umbilical cord mesenchymal stem cells according to the present invention;
fig. 4 is a schematic view of a bottom perspective structure of a placement plate in a centrifugal device for separating umbilical cord mesenchymal stem cells according to the present invention;
fig. 5 is a schematic structural diagram of a first push plate and a pressurizing plate in a centrifugal device for umbilical cord mesenchymal stem cell separation according to the present invention;
fig. 6 is a schematic structural diagram of a lifting plate and a second push plate in a centrifuge for separating umbilical cord mesenchymal stem cells according to the present invention.
Illustration of the drawings:
1. a fixing box; 2. a centrifugal mechanism; 21. a U-shaped plate; 22. a pressing lever; 23. a U-shaped rod; 24. a first triangular block; 25. a second triangular block; 26. a first spring; 27. a first slide bar; 28. a slide chamber; 29. a ball bearing; 210. a support plate; 211. a second spring; 212. a third spring; 213. placing the plate; 214. placing holes; 215. a first card slot; 216. a chute; 217. a second card slot; 3. a first rotating shaft; 4. a suction blower mechanism; 41. a cover plate; 42. a clamping block; 43. a base plate; 44. a second rotating shaft; 45. a fan blade; 46. a suction hole; 47. a housing; 48. an air duct; 49. a gas injection hole; 410. a gas storage shell; 5. a stabilizing mechanism; 51. lifting the plate; 52. a connecting rod; 53. a first push plate; 54. a support bar; 55. a pressing plate; 56. a second slide bar; 57. a fourth spring; 58. a third slide bar; 59. a second push plate; 510. a handle; 511. a through hole; 6. a pillar; 7. positioning holes; 8. a base; 9. an electric motor.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-6, the present invention provides a technical solution: a centrifugal device for umbilical cord mesenchymal stem cell separation comprises a fixed box 1, wherein a centrifugal mechanism 2 is arranged in the fixed box 1, an air suction and blowing mechanism 4 is arranged on the upper side of the fixed box 1, the air suction and blowing mechanism 4 comprises a cover plate 41 arranged on the fixed box 1, a second bearing is clamped on the upper surface of the cover plate 41, a second rotating shaft 44 is sleeved in the second bearing in a sleeved mode, a backing plate 43 is fixedly connected to the bottom end of the second rotating shaft 44, clamping blocks 42 are fixedly connected to four corners of the lower surface of the backing plate 43 and clamped into first clamping grooves 215 formed in the upper surface of a placing plate 213, fan blades 45 are fixedly connected to the outer surface of the second rotating shaft 44 and located in a cover shell 47, the cover shell 47 is fixedly connected to the upper surface of the cover plate 41, a plurality of air suction holes 46 are uniformly formed in the outer surface of the cover shell 47, an air guide pipe 48 is communicated with the upper surface of the cover shell 47, the air guide pipe 48 is a three-way pipe, the other two ends of the air duct 48 penetrate through the base 8 and are communicated with an air storage shell 410, the air storage shell 410 is fixedly connected to the inner wall of the base 8, and a plurality of air injection holes 49 are formed in the outer surface of the base 8;
the specific implementation mode is as follows: air-blast mechanism 4 breathes in through the setting, wherein fixture block 42 card is gone into to first draw-in groove 215, when U-shaped board 21 with place board 213 when rotatory together, it can drive backing plate 43 rotatory to place board 213, backing plate 43 can push down the test tube simultaneously and fix the test tube is stable, backing plate 43 can drive flabellum 45 rotatory through second pivot 44 when rotatory, can inhale the air when flabellum 45 is rotatory, the air can enter into to the housing 47 in through suction hole 46, the inside air of housing 47 can enter into to two air storage shell 410 in through air duct 48 afterwards, the air in the air storage shell 410 can spout through fumarole 49, reach the purpose to the motor 9 cooling, avoid motor 9 long-time work can't effectively dispel the heat and influence work.
The left side and the right side of the lower surface of the fixed box 1 are fixedly connected with a base 8 through a pillar 6, the outer side of the fixed box 1 is fixedly connected with a stabilizing mechanism 5, the stabilizing mechanism 5 is sleeved on the outer side of the pillar 6, the stabilizing mechanism 5 comprises a second slide bar 56 fixedly connected on the left side and the right side of the fixed box 1, the outer side of the second slide bar 56 is sleeved with a second slide sleeve, the outer side of the second slide sleeve is clamped with a first push plate 53, one side surface of the first push plate 53 away from the fixed box 1 is fixedly connected with a squeezing plate 55 through two support bars 54, the lower surface of the first push plate 53 is fixedly connected with a first hinged sheet, the first hinged sheet is hinged with a connecting rod 52, the other end of the connecting rod 52 is hinged with a second hinged sheet, the second hinged sheet is fixedly connected on the upper surface of the lifting plate 51, the left side and the right side of the upper surface of the lifting plate 51 are clamped with a limiting sleeve, and the limiting sleeve is sleeved on the outer side of the pillar 6, the upper surface of the lifting plate 51 is provided with a through hole 511, the first rotating shaft 3 is positioned in the through hole 511, the left side and the right side of the back surface of the lifting plate 51 are fixedly connected with a third sliding rod 58, the outer side of the third sliding rod 58 is sleeved with a third sliding sleeve which is clamped on the front surface of a second push plate 59, the outer side of the third sliding rod 58 is sleeved with a fourth spring 57, two ends of the fourth spring 57 are respectively fixedly connected with the back surface of the third sliding sleeve and the outer surface of the third sliding rod 58, the back surface of the second push plate 59 is fixedly connected with a handle 510, the left side and the right side of the front surface of the second push plate 59 are fixedly connected with clamping rods which are clamped into positioning holes 7 formed in the outer surface of the strut 6, the number of the positioning holes 7 is multiple, the positioning holes 7 are uniformly arranged downwards, the inside of the base 8 is fixedly connected with a motor 9, an output shaft of the motor 9 penetrates through the upper surface of the base 8 and is fixedly connected with the first rotating shaft 3, the top end of the first rotating shaft 3 is connected with the lower surface of the centrifugal mechanism 2, and the first rotating shaft 3 is sleeved in a first bearing clamped on the lower surface of the fixed box 1;
the specific implementation mode is as follows: by arranging the stabilizing mechanism 5, when the stability of the fixing box 1 needs to be maintained, the lifting plate 51 is controlled to move upwards, the lifting plate 51 can drive one ends of the two connecting rods 52 to move upwards, the other end of the connecting rod 52 can drive the first push plate 53 to move, the first push plate 53 can drive the extrusion plates 55 to move through the supporting rod 54, the two extrusion plates 55 can be respectively attached to two external objects, the shaking degree of the fixing box 1 is effectively reduced, the stability of the fixing box 1 is maintained, wherein the fourth spring 57 can push the second push plate 59 to move through the elastic extension of the fourth spring 57, the second push plate 59 can drive the clamping rod to be clamped into the positioning hole 7, the position of the lifting plate 51 can be fixed, the plurality of positioning holes 7 are arranged, the clamping rod can be clamped into different positioning holes 7, at the moment, the two extrusion plates 55 can respectively move to different positions to attach to external objects at different distances, the purpose of fixing the fixing box 1 is achieved according to the environment requirement.
The centrifugal mechanism 2 comprises a U-shaped plate 21, a plurality of balls 29 are fixedly connected to the outer surface of the U-shaped plate 21, the balls 29 are slidably connected in an annular groove formed in the inner wall of the fixing box 1, the lower surface of the U-shaped plate 21 is fixedly connected with the top end of the first rotating shaft 3, grooves are formed in the left side surface and the right side surface of the U-shaped plate 21, U-shaped cavities are formed in the left side surface and the right side surface of the inner part of the U-shaped plate 21, U-shaped rods 23 are arranged in the U-shaped cavities, third springs 212 are fixedly connected to the front side surface and the rear side surface of the lower surface of each U-shaped rod 23, first triangular blocks 24 are fixedly connected to the front side surface and the rear side surface of the upper surface of each U-shaped rod 23, second triangular blocks 25 are attached to the inclined surfaces of the two first triangular blocks 24, first sliding rods 27 are fixedly connected to the opposite surfaces of the two second triangular blocks 25, first sliding sleeves are provided with first sliding sleeves outside the first sliding sleeves, the first sliding sleeves are fixedly connected in sliding cavities 28 formed in the grooves, a first spring 26 is sleeved on the outer side of the first sliding rod 27, two ends of the first spring 26 are fixedly connected with opposite surfaces of the first sliding sleeve and the second triangular block 25 respectively, a containing cavity is formed in the top of the U-shaped plate 21, a pressing rod 22 is arranged in the containing cavity, one end of the pressing rod 22 is fixedly connected with the back surface of the U-shaped rod 23, the other end of the pressing rod 22 extends to the upper side of the U-shaped plate 21, a supporting plate 210 is fixedly connected to the lower surface of the inner wall of the groove through a second spring 211, the outer surface of the supporting plate 210 is attached to the inner wall of the groove, a placing plate 213 is placed in the groove, a plurality of placing holes 214 are formed in the placing plate 213, an inclined groove 216 is formed in the position, corresponding to one end of the first sliding rod 27, of the side surface of the placing plate 213 is provided with a second clamping groove 217 in the position corresponding to the inclined groove 216, and the second clamping groove 217 is located on the upper side of the inclined groove 216;
the specific implementation mode is as follows: by arranging the centrifugal mechanism 2, a plurality of test tubes can be placed in the plurality of placing holes 214, so that the plurality of test tubes can be centrifuged at the same time during centrifugal operation, when the same batch of test tubes need to be taken out after centrifugation is finished, the pressing rod 22 is controlled to move downwards, the pressing rod 22 drives the U-shaped rod 23 to move downwards, the U-shaped rod 23 can drive the first triangular block 24 to move downwards, at the moment, the first triangular block 24 cannot extrude the second triangular block 25, meanwhile, the first spring 26 can push the second triangular block 25 to move through self elastic extension, the second triangular block 25 can drive the first sliding rod 27 to move, one end of the first sliding rod 27 is separated from the second clamping groove 217, meanwhile, the second spring 211 can push the supporting plate 210 to move upwards through self elastic extension, the supporting plate 210 can push the placing plate 213 from the groove, and people can take out the placing plate 213 and take out all centrifuged test tubes at the same time conveniently, then, people put another placing plate 213 prepared in advance into the two grooves and install the placing plate 213, and a plurality of test tubes to be centrifuged are already filled on the other placing plate 213, so that the efficiency of test tube detachment and installation is greatly improved, and the use of people is facilitated, by arranging the chute 216, the placing plate 213 is placed in the grooves and then the placing plate 213 is pressed downwards, under the action of the chute 216, one end of the first slide bar 27 is positioned in the chute 216, when the placing plate 213 moves downwards, the two first slide bars 27 can be extruded and separated from each other, and when the placing plate 213 continues to move downwards, when the second clamping groove 217 moves to the first slide bar 27, the third spring 212 can push the U-shaped bar 23 to move upwards through the elastic extension of the third spring 212, at the moment, the first triangular block 24 extrudes the second triangular block 25, and simultaneously, the second triangular block 25 moves and brings one end of the first slide bar 27 into the second clamping groove 217, the placing plate 213 is well fixed and the operation is very convenient.
The working principle is as follows: when the test tube centrifuge is used, a plurality of test tubes can be placed in the plurality of placing holes 214, so that the plurality of test tubes can be centrifuged simultaneously during centrifugation, then the upper cover plate 41 is covered to control the motor 9 to work, the first rotating shaft 3 can drive the U-shaped plate 21 to rotate for centrifugation, the fixture block 42 is clamped into the first clamping groove 215, when the U-shaped plate 21 and the placing plate 213 rotate together, the placing plate 213 can drive the backing plate 43 to rotate, the backing plate 43 can press the test tubes to stably fix the test tubes, the backing plate 43 can drive the fan blades 45 to rotate through the second rotating shaft 44, the fan blades 45 can suck air when rotating, the air can enter the housing 47 through the air suction holes 46, then the air in the housing 47 can enter the two air storage shells 410 through the air guide tubes 48, the air in the air storage shells 410 can be ejected through the air ejection holes 49, the purpose of cooling the motor 9 is achieved, after centrifugation, the cover plate 41 is opened, and then the control rod 22 moves downwards, pressing rod 22 drives U-shaped pole 23 and moves down, U-shaped pole 23 can drive first three hornblocks 24 and move down, first three hornblocks 24 can not form the extrusion to second three hornblocks 25 this moment, first spring 26 can push up the removal of second three hornblocks 25 through self elasticity extension simultaneously, second three hornblocks 25 can drive first slide bar 27 and move, let the one end of first slide bar 27 break away from second draw-in groove 217, second spring 211 can push up through self elasticity extension and move backup pad 210 rebound simultaneously, backup pad 210 can push up from the recess placing board 213, make things convenient for people to take out and take out all centrifugal good test tubes simultaneously placing board 213, people will prepare in advance another placing board 213 put into two recesses and install well afterwards.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. The centrifugal device for separating umbilical cord mesenchymal stem cells comprises a fixing box (1) and is characterized in that a centrifugal mechanism (2) is arranged in the fixing box (1), an air suction and blowing mechanism (4) is arranged on the upper side of the fixing box (1), bases (8) are fixedly connected to the left side and the right side of the lower surface of the fixing box (1) through pillars (6), stabilizing mechanisms (5) are fixedly connected to the outer side of the fixing box (1), the stabilizing mechanisms (5) are sleeved on the outer side of the pillars (6), a motor (9) is fixedly connected to the inner portion of the bases (8), an output shaft of the motor (9) penetrates through the upper surface of the bases (8) and is fixedly connected with a first rotating shaft (3), the top end of the first rotating shaft (3) is connected with the lower surface of the centrifugal mechanism (2), and the first rotating shaft (3) is sleeved in a first bearing clamped with the lower surface of the fixing box (1);
the centrifugal mechanism (2) comprises a U-shaped plate (21), a plurality of balls (29) are fixedly connected to the outer surface of the U-shaped plate (21), the balls (29) are slidably connected into an annular groove formed in the inner wall of the fixing box (1), the lower surface of the U-shaped plate (21) is fixedly connected with the top end of the first rotating shaft (3), grooves are formed in the left side surface and the right side surface of the U-shaped plate (21), U-shaped cavities are formed in the left side and the right side surface of the inside of the U-shaped plate (21), U-shaped rods (23) are arranged in the U-shaped cavities, third springs (212) are fixedly connected to the front side and the rear side of the lower surface of each U-shaped rod (23), first triangular blocks (24) are fixedly connected to the front side and the rear side of the upper surface of each U-shaped rod (23), second triangular blocks (25) are respectively attached to inclined planes of the two first triangular blocks (24), first sliding rods (27) are respectively fixedly connected to opposite faces of the two second triangular blocks (25), the outer side of the first sliding rod (27) is sleeved with a first sliding sleeve, the first sliding sleeve is fixedly connected in a sliding cavity (28) formed in the groove, the outer side of the first sliding rod (27) is sleeved with a first spring (26), two ends of the first spring (26) are fixedly connected with opposite surfaces of the first sliding sleeve and the second triangular block (25) respectively, the top of the U-shaped plate (21) is provided with a containing cavity, a pressing rod (22) is arranged in the containing cavity, one end of the pressing rod (22) is fixedly connected with the back surface of the U-shaped rod (23), the other end of the pressing rod (22) extends to the upper side of the U-shaped plate (21), the lower surface of the inner wall of the groove is fixedly connected with a supporting plate (210) through a second spring (211), the outer surface of the supporting plate (210) is attached to the inner wall of the groove, a placing plate (213) is placed in the groove, a plurality of placing holes (214) are formed in the placing plate (213), the lower surface of the placing plate (213) is provided with an inclined groove (216) corresponding to one end of the first sliding rod (27), the side surface of the placing plate (213) is provided with a second clamping groove (217) corresponding to the inclined groove (216), and the second clamping groove (217) is positioned on the upper side of the inclined groove (216).
2. The centrifugal device for umbilical cord mesenchymal stem cell separation according to claim 1, wherein the air suction and blowing mechanism (4) comprises a cover plate (41) arranged on the fixing box (1), the upper surface of the cover plate (41) is connected with a second bearing in a clamping manner, the second bearing is sleeved with a second rotating shaft (44), the bottom end of the second rotating shaft (44) is fixedly connected with a base plate (43), the four corners of the lower surface of the base plate (43) are fixedly connected with clamping blocks (42), and the clamping blocks (42) are clamped into first clamping grooves (215) formed in the upper surface of the placing plate (213).
3. The centrifugal device for umbilical cord mesenchymal stem cell separation according to claim 2, wherein the outer surface of the second rotating shaft (44) is fixedly connected with a fan blade (45), the fan blade (45) is located in the housing (47), the housing (47) is fixedly connected to the upper surface of the cover plate (41), a plurality of air suction holes (46) are uniformly formed in the outer surface of the housing (47), the upper surface of the housing (47) is communicated with an air duct (48), the air duct (48) is a three-way pipe, the other two ends of the air duct (48) are communicated with the inside of the base (8) and an air storage shell (410), the air storage shell (410) is fixedly connected to the inner wall of the base (8), and a plurality of air injection holes (49) are formed in the outer surface of the base (8).
4. The centrifugation device for separating umbilical cord mesenchymal stem cells according to claim 1, wherein the stabilizing mechanism (5) comprises a second slide bar (56) fixedly connected to the left and right sides of the fixing box (1), a second slide sleeve is sleeved on the outer side of the second slide bar (56), a first push plate (53) is clamped on the outer side of the second slide sleeve, and a side surface of the first push plate (53) far away from the fixing box (1) is fixedly connected with a pressing plate (55) through two support bars (54).
5. The centrifugation device for separating umbilical cord mesenchymal stem cells according to claim 4, wherein the lower surface of the first push plate (53) is fixedly connected with a first hinged sheet, the first hinged sheet is hinged with a connecting rod (52), the other end of the connecting rod (52) is hinged with a second hinged sheet, the second hinged sheet is fixedly connected with the upper surface of the lifting plate (51), the left side and the right side of the upper surface of the lifting plate (51) are respectively clamped with a limiting sleeve, the limiting sleeves are sleeved on the outer side of the support column (6), the upper surface of the lifting plate (51) is provided with a through hole (511), and the first rotating shaft (3) is positioned in the through hole (511).
6. The centrifugation device for separating umbilical cord mesenchymal stem cells according to claim 5, wherein the centrifugation device comprises a first centrifuge, lift equal fixedly connected with third slide bar (58) in the left and right sides at board (51) the back, the outside cover of third slide bar (58) is equipped with the third sliding sleeve, the front at second push pedal (59) is connected to the third sliding sleeve joint, the outside cover of third slide bar (58) is equipped with fourth spring (57), the both ends of fourth spring (57) are connected with the back of third sliding sleeve and the surface fixed connection of third slide bar (58) respectively, the back fixedly connected with handle (510) of second push pedal, the equal fixedly connected with kelly in the left and right sides that second push pedal (59) is positive, the kelly card is gone into to locating hole (7) that pillar (6) surface was seted up in, the quantity of locating hole (7) is a plurality ofly, a plurality of locating hole (7) down align to grid from last set up.
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| CN202210489571.0A CN114570535B (en) | 2022-05-07 | 2022-05-07 | Centrifugal device for umbilical cord mesenchymal stem cell separation |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109499784A (en) * | 2018-12-11 | 2019-03-22 | 中国人民解放军陆军军医大学第二附属医院 | Micro centrifuge |
| CN111085345A (en) * | 2019-12-10 | 2020-05-01 | 刘付娥 | Laboratory is with lift type cell centrifuge |
| CN111229479A (en) * | 2020-01-28 | 2020-06-05 | 张天明 | Centrifugal device for clinical laboratory |
| CN212560190U (en) * | 2020-04-23 | 2021-02-19 | 广州星乐贸易有限公司 | Equipment for rapidly extracting stem cells |
| CN113198623A (en) * | 2021-06-25 | 2021-08-03 | 南阳师范学院 | Intelligent centrifuge for it is biological experiments |
| CN213943546U (en) * | 2020-12-18 | 2021-08-13 | 浙江省人民医院 | Mesenchymal stem cell separation and extraction device |
| CN215250892U (en) * | 2021-07-05 | 2021-12-21 | 山东维真生物科技有限公司 | Biological cell separation device |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103472241B (en) * | 2008-01-21 | 2015-06-17 | 三星电子株式会社 | Thin-film layered centrifuge device and analysis method using the same |
| DE102019004958A1 (en) * | 2019-07-16 | 2021-01-21 | Thermo Electron Led Gmbh | Centrifuge rotor, retaining ring and retaining ring arrangement therefor, as well as centrifuge |
| EP3825005A1 (en) * | 2019-11-22 | 2021-05-26 | F. Hoffmann-La Roche AG | Bucket insert for use in a centrifuge |
| CN111607508B (en) * | 2020-04-28 | 2023-03-10 | 深圳大学 | Automatic mesenchymal stem cell separation device |
| CN111701731A (en) * | 2020-06-28 | 2020-09-25 | 陈龙刚 | Centrifugal machine for stem cell separation |
| CN112708541A (en) * | 2021-01-03 | 2021-04-27 | 西安蓝华飞腾生物科技有限公司 | Separation equipment for cell exosomes and application method thereof |
-
2022
- 2022-05-07 CN CN202210489571.0A patent/CN114570535B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109499784A (en) * | 2018-12-11 | 2019-03-22 | 中国人民解放军陆军军医大学第二附属医院 | Micro centrifuge |
| CN111085345A (en) * | 2019-12-10 | 2020-05-01 | 刘付娥 | Laboratory is with lift type cell centrifuge |
| CN111229479A (en) * | 2020-01-28 | 2020-06-05 | 张天明 | Centrifugal device for clinical laboratory |
| CN212560190U (en) * | 2020-04-23 | 2021-02-19 | 广州星乐贸易有限公司 | Equipment for rapidly extracting stem cells |
| CN213943546U (en) * | 2020-12-18 | 2021-08-13 | 浙江省人民医院 | Mesenchymal stem cell separation and extraction device |
| CN113198623A (en) * | 2021-06-25 | 2021-08-03 | 南阳师范学院 | Intelligent centrifuge for it is biological experiments |
| CN215250892U (en) * | 2021-07-05 | 2021-12-21 | 山东维真生物科技有限公司 | Biological cell separation device |
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