CN114532356A - 一种杀灭细菌芽孢的组合物以及制备方法和杀灭细菌芽孢的方法 - Google Patents
一种杀灭细菌芽孢的组合物以及制备方法和杀灭细菌芽孢的方法 Download PDFInfo
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Abstract
本申请属于水体消毒技术领域,尤其涉及一种杀灭细菌芽孢的组合物以及制备方法和杀灭细菌芽孢的方法。本申请提供了一种杀灭细菌芽孢的组合物,包括:天然矿物粉末和过硫酸盐;所述天然矿物粉末选自闪锌矿粉末、黄铁矿粉末和赤铁矿粉末中的一种或多种;所述过硫酸盐选自过二硫酸纳、过二硫酸钾、过一硫酸纳和过一硫酸钾中的一种或多种;所述天然矿物粉末和所述过硫酸盐的质量比为(0.5~2.0):1。本申请提供了一种杀灭细菌芽孢的组合物以及制备方法和杀灭细菌芽孢的方法,可有效杀灭细菌芽孢,解决现有常规消毒技术对芽孢的杀灭效果有限的技术问题。
Description
技术领域
本申请属于水体消毒技术领域,尤其涉及一种杀灭细菌芽孢的组合物以及制备方法和杀灭细菌芽孢的方法。
背景技术
水传播疾病通过病毒、细菌和原生动物等病原微生物传播,严重危害人类健康。在各类病原微生物中,细菌芽孢是特定细菌在外界不良刺激下细胞质高度浓缩脱水所形成的一种抗逆性很强的休眠体,其对各种环境压力,包括极端温度,压力和辐射,具有极强的抵抗力,并且可以与有毒化学物质共存。一旦休眠芽孢暴露在适宜环境条件下,它就可以恢复生长过程并传播疾病。然而常规消毒技术如氯消毒对芽孢的杀灭效果极其有限,臭氧消毒成本高昂,紫外线消毒无持续消毒功能并杀灭效果局限等。这些常规消毒技术无法有效杀灭芽孢。因此,高效杀灭细菌芽孢的技术方法在水消毒领域非常重要,是近几年水消毒领域的研究热点之一。
发明内容
有鉴于此,本申请提供了一种杀灭细菌芽孢的组合物以及制备方法和杀灭细菌芽孢的方法,可有效杀灭细菌芽孢,解决现有常规消毒技术对芽孢的杀灭效果有限的技术问题。
本申请第一方面提供了一种杀灭细菌芽孢的组合物,包括:
天然矿物粉末和过硫酸盐;
所述天然矿物粉末选自闪锌矿粉末、黄铁矿粉末和赤铁矿粉末中的一种或多种;所述过硫酸盐选自过二硫酸纳、过二硫酸钾、过一硫酸纳和过一硫酸钾中的一种或多种;
所述天然矿物粉末和所述过硫酸盐的质量比为(0.5~2.0):1。
另一实施例中,所述天然矿物粉末的粒径为0.1~40μm。
具体的,所述天然矿物粉末为微米级天然矿物,原矿经过机械粉碎、研磨,通过过筛(340目孔筛)。
本申请第二方面提供了所述杀灭细菌的组合物的制备方法,包括以下步骤:
将天然矿物粉末和过硫酸盐混合,得到杀灭细菌芽孢的组合物;
所述天然矿物粉末选自闪锌矿粉末、黄铁矿粉末和赤铁矿粉末中的一种或多种;所述过硫酸盐选自过二硫酸纳、过二硫酸钾、过一硫酸纳和过一硫酸钾中的一种或多种;
所述天然矿物粉末和所述过硫酸盐的质量比为(0.5~2.0):1。
具体的,所述天然矿物粉末和所述过硫酸盐的质量比为(1~2):1。
本申请第三方面提供了杀灭细菌芽孢的方法,包括:
步骤1、将所述组合物与含有细菌芽孢的水相体系混合,得到混合溶液;
步骤2、调节所述混合溶液的温度至20℃~80℃,且光照处理所述混合溶液,杀灭所述混合溶液的细菌芽孢。
具体的,所述混合溶液的温度为30℃~60℃、40℃~60℃、50℃~60℃、30℃、40℃、50℃或60℃。
另一实施例中,所述天然矿物粉末在所述水相体系的浓度为0.1~2g/L。
具体的,所述天然矿物粉末在所述水相体系的浓度为1~2g/L;所述过硫酸盐在所述水相体系的浓度为1~2g/L。
另一实施例中,所述细菌芽孢为产芽孢杆菌的休眠孢子。
另一实施例中,所述细菌芽孢为枯草芽孢杆菌芽孢、蜡样芽孢杆菌芽孢或地衣芽孢杆菌芽孢中的一种或多种。
另一实施例中,所述细菌芽孢在所述水相体系的浓度为1×104CFU/mL~1×107CFU/mL。
另一实施例中,所述光源为氙灯光源、LED光源或自然太阳光源。
另一实施例中,所述光照处理的时间为4~8h,所述光照处理的强度为60~80mW/cm2。
本申请的杀灭细菌芽孢为光催化消毒方法,光催化过程产生的光生电子激活过硫酸盐,即过硫酸盐在天然矿物和光照活化条件下可形成过硫酸根自由基(·SO4-),具有比·OH更高的氧化还原电位和稳定性,因此,在天然矿物光催化体系中引入过硫酸盐可大幅强化传统光催化体系的氧化能力,从而实现对顽固病原微生物如细菌芽孢的高效杀灭。本申请方法利用微米级天然矿物作为光催化剂,在可见光下一定温度范围(20℃~80℃)内活化过硫酸盐,形成过硫酸根自由基等活性物种,从而实现对水体中顽固病原微生物—细菌芽孢的有效杀灭及矿化。本申请的天然矿物粉末可多次重复循环利用。本申请方法对水中多种产芽孢菌均有高效杀灭能力,且具有催化剂成本低、来源广泛、可直接利用太阳能、不产生二次污染的优势,在顽固病原微生物杀灭及水体消毒领域具有广阔应用前景。
附图说明
为了更清楚地说明本申请实施例或现有技术中的技术方案,下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍。
图1为本申请实施例1提供的不同组合物在不同条件杀灭细菌芽孢的效率对比图;
图2为本申请实施例1提供的组合物在不同温度下杀灭细菌芽孢的效率对比图;
图3为本申请实施例提供的组合物在不同光照强度下杀灭细菌芽孢的效率对比图;
图4为本申请实施例提供的不同天然闪锌矿粉末浓度和不同过硫酸盐浓度下杀灭细菌芽孢的效率对比图。
具体实施方式
本申请提供了一种杀灭细菌芽孢的组合物以及制备方法和杀灭细菌芽孢的方法,用于解决现有技术中对芽孢的杀灭效果有限的技术缺陷。
下面将对本申请实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本申请一部分实施例,而不是全部的实施例。基于本申请中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本申请保护的范围。
其中,以下实施例所用原料或试剂均为市售或自制。
实施例1
本申请实施例提供了利用组合物杀灭细菌芽孢的方法,具体包括:
1、制备杀灭细菌芽孢的组合物,包括:天然矿物粉末和过硫酸盐。将天然闪锌矿进行机械粉碎、研磨、过筛(340目孔筛),获得微米级天然闪锌矿粉末,其粒径小于40μm,得到天然闪锌矿粉末;调节天然闪锌矿粉末在水相体系的添加浓度为2g/L,天然矿物闪锌矿粉末与过一硫酸钾的质量比为1:1,将天然闪锌矿粉末与过一硫酸钾混合,得到组合物。
2、将上述组合物(包括天然闪锌矿粉末和过硫酸盐)加入含有枯草芽孢杆菌孢子的水相体系中;光催化杀灭芽孢性能测试在石英玻璃反应器中进行。选取枯草芽孢杆菌孢子作为目标杀灭芽孢。
将枯草芽孢杆菌孢子用无菌生理盐水稀释成浓度为1×107cfu/mL的芽孢溶液。将100mg天然闪锌矿粉末与100mg过一硫酸钾加入50mL上述芽孢溶液中,使天然闪锌矿粉末和过一硫酸钾浓度均为2g/L,得到混合溶液。
3、调节混合溶液的温度,打开光源进行光照处理。
1)在上述混合溶液中,调节反应容器温度为60℃,置于暗处搅拌30min,然后打开配备可见光滤光片的氙灯光源(光源>400nm)开始反应,每隔1小时取样,利用平板计数法计算菌落数目。如图1所示,在光照条件下,天然闪锌矿粉末/过一硫酸钾可在6h内完全杀灭1×107cfu/mL的芽孢(图1标记为天然矿物/过硫酸盐/光照),效果明显高于黑暗下天然闪锌矿粉末/过一硫酸钾和天然闪锌矿粉末的单独光催化杀灭芽孢效率。图1的光对照为不加入组合物的含有枯草芽孢杆菌孢子的水相体系;图1的天然矿物(暗对照)为含有枯草芽孢杆菌孢子的水相体系中添加100mg天然闪锌矿粉末后在黑暗中处理的对照;图1的天然矿物/过硫酸盐为含有枯草芽孢杆菌孢子的水相体系中添加上述组合物后在黑暗中处理的对照。
2)在上述步骤2的混合溶液中,分别调节反应容器温度为20℃、30℃、40℃、50℃和60℃,分别置于暗处搅拌30min,然后打开配备可见光滤光片的氙灯光源(光源>400nm)开始反应,每隔1小时取样,利用平板计数法计算菌落数目。如图2所示,反应容器内温度越高,天然闪锌矿粉末活化过一硫酸钾效果更加明显,杀灭芽孢效率更加明显,其中,高温能够促进芽孢萌发,使得芽孢转变为营养细胞,十分有利于芽孢杀灭。
3)在上述步骤2的混合溶液中,调节反应容器温度为60℃,分别置于暗处搅拌30min,然后打开配备可见光滤光片的氙灯光源(光源>400nm),分别调节光强为黑暗、10mW/cm2、40mW/cm2和70mW/cm2,然后开始反应,每隔1小时取样,利用平板计数法计算菌落数目。如图3所示,随着光强的增加,芽孢杀灭效果更为有效。
4)按照上述方法,调节天然闪锌矿粉末和过一硫酸钾添加的浓度,测定不同天然闪锌矿粉末和过一硫酸钾浓度对芽孢的杀灭效果,第二组为天然闪锌矿粉末添加的浓度为1g/L,过一硫酸钾添加的浓度2g/L;第三组为天然闪锌矿粉末添加的浓度为2g/L,过一硫酸钾添加的浓度1g/L;第四组为天然闪锌矿粉末添加的浓度为1g/L,过一硫酸钾添加的浓度1g/L;第五组为天然闪锌矿粉末添加的浓度为0.5g/L,过一硫酸钾添加的浓度0.5g/L;第六组为天然闪锌矿粉末添加的浓度为0.1g/L,过一硫酸钾添加的浓度0.1g/L。调节反应容器温度为60℃,置于暗处搅拌30min,然后打开配备可见光滤光片的氙灯光源(光源>400nm)开始反应,每隔1小时取样,利用平板计数法计算菌落数目。如图4所示,2g/L闪锌矿/2g/L过一硫酸钾杀灭芽孢效果最有效。实验结果表明,本申请利用天然闪锌矿粉末光活化过硫酸盐杀灭细菌芽孢的方法是一种高效杀灭细菌芽孢方法,效果显著高于传统光催化杀灭细菌芽孢。
实施例2
本申请实施例提供了利用组合物杀灭细菌芽孢的方法,具体包括:
1、制备杀灭细菌芽孢的组合物,包括:天然赤铁矿粉末和过硫酸盐。将天然赤铁矿原矿进行机械粉碎、研磨、过筛(340目孔筛),获得微米级赤铁矿粉末,其粒径小于40μm;调节天然赤铁矿粉末的添加浓度为0.8g/L,天然矿物赤铁矿粉末与过二硫酸钾的质量比为2:1,将天然赤铁矿粉末与过二硫酸钾混合,得到组合物。
2、将上述组合物(包括天然赤铁矿粉末和过硫酸盐)加入含有蜡样芽孢杆菌芽孢的水相体系中;光催化杀灭芽孢性能测试在石英玻璃反应器中进行。选取蜡样芽孢杆菌孢子作为目标杀灭芽孢。
将蜡样芽孢杆菌孢子用无菌生理盐水稀释成浓度为1×105cfu/mL的芽孢溶液。将40mg天然赤铁矿粉末与20mg过二硫酸钾加入50mL上述芽孢溶液中,使天然赤铁矿粉末和过二硫酸钾浓度分别为0.8g/L和0.4g/L,得到混合溶液。
3、调节体系温度,打开光源进行光照处理。
在上述混合溶液中,调节反应容器温度为50℃,置于暗处搅拌30min,然后打开LED光源开始反应,每隔1小时取样,利用平板计数法计算菌落数目。实验结果显示,1×105cfu/mL浓度的蜡样芽孢杆菌芽孢在该***中可在6h内被完全杀灭,表明利用天然赤铁矿光活化过二硫酸钾也可杀灭蜡样芽孢杆菌芽孢,效果显著。
实施例3
本申请实施例提供了利用组合物杀灭细菌芽孢的方法,具体包括:
1、制备杀灭细菌芽孢的组合物,包括:天然黄铁矿原矿进行机械粉碎、研磨、过筛(340目孔筛),获得微米级黄铁矿粉末,其粒径小于40μm;调节天然黄铁矿粉末的添加浓度为1.2g/L,天然黄铁矿粉末与过二硫酸钠的质量比为0.5:1,将天然黄铁矿粉末与过二硫酸钠混合,得到组合物。
2、将上述组合物(包括天然黄铁矿粉末和过硫酸盐)加入含有地衣芽孢杆菌孢子的水相体系中;光催化杀灭芽孢性能测试在石英玻璃反应器中进行。选取地衣芽孢杆菌孢子作为目标杀灭芽孢。将地衣芽孢杆菌孢子用无菌生理盐水稀释成浓度为1×106cfu/mL的芽孢溶液。将60mg天然黄铁矿粉末与120mg过二硫酸钠加入50mL上述芽孢溶液中,使天然黄铁矿粉末和过二硫酸钠浓度分别为1.2g/L和2.4g/L,得到混合溶液。
3、调节体系温度,打开光源进行光照处理。
在上述混合溶液中,调节反应容器温度为60℃,置于暗处搅拌30min,然后打开自然太阳光源开始反应,每隔1小时取样,利用平板计数法计算菌落数目。实验结果显示,1×106cfu/mL浓度的地衣芽孢杆菌孢子在该***中可在6h内被完全杀灭,表明利用天然黄铁矿粉末光活化过二硫酸钠也可杀灭地衣芽孢杆菌芽孢,效果显著高于传统光催化杀灭细菌芽孢。
与现有技术相比,本申请的有益效果是:本申请利用自然界广泛存在的天然矿物作为催化剂,克服了传统光催化杀菌技术采用人工合成催化剂的高成本,并且耦合过硫酸盐克服了传统技术的氧化能力弱,对芽孢的杀灭率低的缺陷。本申请方法对水中多种细菌芽孢均有高效杀灭能力,且具有催化剂成本低、来源广泛、可直接利用太阳能、不产生二次污染的优势,在顽固病原微生物杀灭及水体消毒领域具有广阔应用前景。
以上所述仅是本申请的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本申请原理的前提下,还可以作出若干改进和润饰,这些改进和润饰也应视为本申请的保护范围。
Claims (10)
1.一种杀灭细菌芽孢的组合物,其特征在于,包括:
天然矿物粉末和过硫酸盐;
所述天然矿物粉末选自闪锌矿粉末、黄铁矿粉末和赤铁矿粉末中的一种或多种;所述过硫酸盐选自过二硫酸纳、过二硫酸钾、过一硫酸纳和过一硫酸钾中的一种或多种;
所述天然矿物粉末和所述过硫酸盐的质量比为(0.5~2.0):1。
2.根据权利要求1所述的组合物,其特征在于,所述天然矿物粉末的粒径为0.1~40μm。
3.一种杀灭细菌芽孢的组合物的制备方法,其特征在于,包括以下步骤:
将天然矿物粉末和过硫酸盐混合,得到杀灭细菌芽孢的组合物;
所述天然矿物粉末选自闪锌矿粉末、黄铁矿粉末和赤铁矿粉末中的一种或多种;所述过硫酸盐选自过二硫酸纳、过二硫酸钾、过一硫酸纳和过一硫酸钾中的一种或多种;
所述天然矿物粉末和所述过硫酸盐的质量比为(0.5~2.0):1。
4.一种杀灭细菌芽孢的方法,其特征在于,包括:
步骤1、将权利要求1或2所述的组合物与含有细菌芽孢的水相体系混合,得到混合溶液;
步骤2、调节所述混合溶液的温度至20℃~80℃,且光照处理所述混合溶液,杀灭所述混合溶液的细菌芽孢。
5.根据权利要求4所述的方法,其特征在于,所述天然矿物粉末在所述水相体系的浓度为0.1~2g/L。
6.根据权利要求4所述的方法,其特征在于,所述细菌芽孢为产芽孢杆菌的休眠孢子。
7.根据权利要求6所述的方法,其特征在于,所述细菌芽孢为枯草芽孢杆菌芽孢、蜡样芽孢杆菌芽孢或地衣芽孢杆菌芽孢中的一种或多种。
8.根据权利要求4所述的方法,其特征在于,所述细菌芽孢在所述水相体系的浓度为1×104CFU/mL~1×107CFU/mL。
9.根据权利要求4所述的方法,其特征在于,所述光源为氙灯光源、LED光源或自然太阳光源。
10.根据权利要求4所述的方法,其特征在于,所述光照处理的时间为4~8h,所述光照处理的强度为60~80mW/cm2。
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