CN114521558A - Sweet corn seed soaking agent and preparation method and application thereof - Google Patents
Sweet corn seed soaking agent and preparation method and application thereof Download PDFInfo
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- CN114521558A CN114521558A CN202210173358.9A CN202210173358A CN114521558A CN 114521558 A CN114521558 A CN 114521558A CN 202210173358 A CN202210173358 A CN 202210173358A CN 114521558 A CN114521558 A CN 114521558A
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- sweet corn
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- corn seed
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- seed soaking
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- 235000002017 Zea mays subsp mays Nutrition 0.000 title claims abstract description 132
- 241000482268 Zea mays subsp. mays Species 0.000 title claims abstract description 128
- 238000002791 soaking Methods 0.000 title claims abstract description 76
- 238000002360 preparation method Methods 0.000 title description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 65
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims abstract description 28
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229920001661 Chitosan Polymers 0.000 claims abstract description 17
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 15
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229960003237 betaine Drugs 0.000 claims abstract description 15
- 239000002509 fulvic acid Substances 0.000 claims abstract description 15
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 15
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims abstract description 14
- 229940063673 spermidine Drugs 0.000 claims abstract description 14
- IXVMHGVQKLDRKH-QHBHMFGVSA-N 24-Epibrassinolide Natural products C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-QHBHMFGVSA-N 0.000 claims abstract description 13
- 239000005781 Fludioxonil Substances 0.000 claims abstract description 13
- MUJOIMFVNIBMKC-UHFFFAOYSA-N fludioxonil Chemical compound C=12OC(F)(F)OC2=CC=CC=1C1=CNC=C1C#N MUJOIMFVNIBMKC-UHFFFAOYSA-N 0.000 claims abstract description 13
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 11
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims abstract 4
- 239000000203 mixture Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 10
- 238000009331 sowing Methods 0.000 claims description 9
- 239000011259 mixed solution Substances 0.000 claims description 8
- 238000007865 diluting Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims 2
- 238000010790 dilution Methods 0.000 claims 2
- 230000012010 growth Effects 0.000 abstract description 17
- 230000035784 germination Effects 0.000 abstract description 13
- 230000008569 process Effects 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract description 4
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- 230000000694 effects Effects 0.000 description 18
- 239000002689 soil Substances 0.000 description 13
- 238000009472 formulation Methods 0.000 description 10
- 238000012360 testing method Methods 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 235000005822 corn Nutrition 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 4
- 229920000768 polyamine Polymers 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 229920002101 Chitin Polymers 0.000 description 3
- 150000001647 brassinosteroids Chemical class 0.000 description 3
- 238000011161 development Methods 0.000 description 3
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- 241000238631 Hexapoda Species 0.000 description 2
- 230000036579 abiotic stress Effects 0.000 description 2
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- 239000003513 alkali Substances 0.000 description 2
- 230000004790 biotic stress Effects 0.000 description 2
- 230000006353 environmental stress Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000003204 osmotic effect Effects 0.000 description 2
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 description 2
- 125000001460 24-epi-brassinolide group Chemical group 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 239000005700 Putrescine Substances 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
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- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
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- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000015572 biosynthetic process Effects 0.000 description 1
- -1 brassinosteroid compound Chemical class 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
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- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000006196 deacetylation Effects 0.000 description 1
- 238000003381 deacetylation reaction Methods 0.000 description 1
- 230000008641 drought stress Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
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- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000004224 protection Effects 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
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- 239000008107 starch Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a sweet corn steepThe seed agent is characterized by comprising the following components in parts by weight: betaine (20-80 parts), 24-epibrassinolide (0.05-0.15 parts), spermidine (10-50 parts), Ca (NO)3)2(500-3600 portions), fulvic acid (100-200 portions), chitosan (100-200 portions), fludioxonil (10-50 portions) and water (5820-9260 portions). The sweet corn seed soaking agent is safe for sweet corn seeds, is harmless and residue-free in the using process, can effectively improve the topsoil capability, the emergence rate and the growth vigor of the sweet corn, improves the capabilities of the sweet corn in germination and emergence periods, such as drought resistance, waterlogging resistance, high temperature resistance, disease resistance and the like, effectively improves the seedling-stage quality of the sweet corn, and is simple in using method, small in using amount and low in cost.
Description
Technical Field
The invention relates to the field of seed soaking agents. More specifically, the invention relates to a sweet corn seed soaking agent, a preparation method and an application thereof.
Background
The endosperm starch of the sweet corn seeds is less, and the nutrition storage is insufficient; meanwhile, the sweet corn seeds are easy to be damaged in the processes of harvesting, processing, storing and transporting, the sugar content of the seeds is high, and the seeds swell too fast to cause leakage. Therefore, the germination capacity and the top soil capacity of the sweet corn seeds are lower than those of common corn, the requirements on soil moisture content and ground temperature are strict, the germination process is more easily infected by pathogenic bacteria, and the problems of seedling shortage, weak seedlings, irregular seedlings and the like are more easily encountered in production. The southern hilly corn area has rich photo-thermal resources and is suitable for corn growth. But also has the adverse conditions of frequent stress such as drought, waterlogging, high temperature and the like, heavy and easy hardening of soil, serious diseases, insect pests and weeds and the like. Improving the early-stage environmental stress resistance of the seeds is an important means for ensuring high yield of the corn in the region. Therefore, plant growth regulating substances are required to be applied externally to regulate the growth and development process of plants, improve the stress resistance of the plants, increase the yield and improve the quality.
The seed soaking agent is a seed treating agent containing one or more effective components, has the functions of protection, nutrition, stress resistance and the like, can improve the emergence rate and uniformity of seeds, reduce insect damage and disease damage, improve the stress resistance of crops and the like.
Brassinosteroids (BR) are novel hormones which have important influence on the growth and development of plants, and BR is a natural brassinosteroid compound which is discovered to date and has the strongest physiological activity, can react to the adversity of biotic and abiotic stress, and participate in various physiological processes such as high-temperature stress, low-temperature stress, drought stress, salt stress, waterlogging stress, pesticide stress and the like.
Betaine (GB) is a natural nontoxic osmotic pressure regulating substance, is the most potential osmotic protective agent, and is concerned in plant stress resistance research. The betaine with proper concentration can improve the activity of protective enzyme and the content of chlorophyll in plants and effectively promote the photosynthesis and dry matter accumulation of the plants, thereby relieving the damage of adverse conditions such as high temperature, salt and alkali, drought, hypoxia and the like to the plants.
Polyamines (Polyamines, Pas) such as Putrescine (Put), Spermidine (Spd) and Spermine (Spm) are most commonly closely related to the growth, morphogenesis, senescence, stress resistance and the like of plants. The polyamine is existed in polycation state under physiological pH value condition, and can be firmly combined with nucleic acid, protein and phospholipid with negative charge, so as to maintain stability and integrity of membrane, and the polyamine can be used as proton source to scavenge free radical.
Ca2+Has received much attention as a major messenger in plant cells. Environmental changes can be caused by changes in intracellular Ca2+And transduces extracellular signals into intracellular physiological and biochemical reactions. Many stress conditions such as high temperature, low temperature, mechanical stress, oxidative stress, anoxic stress, hypotonic stress and salt stress can induce intracellular free Ca2+The level rises rapidly. Ca for hindering stress exercise2+The increased level will block the formation of plant stress resistance and artificially increase the intracellular Ca2+The level can induce the expression of resistance related genes and improve the stress resistance of plants.
Fulvic Acid (FA) is a biological stimulator with very obvious application effect in agricultural production, and can effectively increase the activity of various enzymes of seeds, protect the integrity of cell membranes, improve the capability of crops in resisting stress such as high temperature, freezing damage, salt and alkali, drought, diseases and the like and increase the yield and quality of the crops by treating the seeds in a seed soaking or seed dressing mode and the like.
Chitosan (Chltosan), also known as chitosan, is an important derivative of Chitin (Chitin), is converted from Chitin deacetylation, and is a linear sugar of amino polyglucose, with the chemical name of beta- (1,4) -2-amino-2 deoxy-D-glucan (chitosan). The chitosan has the function of regulating the growth and development of plants, and can induce the plants to generate broad-spectrum disease resistance, inhibit the growth of various plant pathogenic bacteria, improve the stress resistance of the plants and the like.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and provide a seed soaking agent for sweet corn to reduce the germination of sweet corn and the influence of abiotic stress and biotic stress during the seedling stage, increase the germination rate of sweet corn, promote the growth of sweet corn and increase the stress tolerance of sweet corn.
To achieve these objects and other advantages in accordance with the present invention, there is provided a sweet corn seed soaking agent comprising the following components in parts by weight: betaine (20-80 parts), 24-epibrassinolide (0.05-0.15 parts), spermidine (10-50 parts), Ca (NO)3)2(500-3600 portions), fulvic acid (100-200 portions), chitosan (100-200 portions), fludioxonil (10-50 portions) and water (5820-9260 portions).
Preferably, the betaine (40-60 parts), 24-epibrassinolide (0.05-0.1 part), spermidine (20-30 parts), Ca (NO)3)2(1000-1200 parts), fulvic acid (100-120 parts), chitosan (120-150 parts), fludioxonil (20-30 parts), and water (8410-8700 parts).
Preferably, the method for preparing the sweet corn seed soaking agent comprises the following steps:
a. mixing Ca (NO)3)2Mixing fulvic acid, chitosan and water, heating, and uniformly stirring to obtain a mixed solution;
b. and d, after the mixed solution obtained in the step a is cooled, adding betaine, 24-epibrassinolide, spermidine and fludioxonil into the mixed solution, and uniformly stirring to obtain the compound.
Preferably, the temperature of the mixing and stirring in the step a is 40-50 ℃.
Preferably, the stirring time in step a is 1 to 2 hours.
Preferably, the stirring time in the step b is 20 to 30 minutes.
Preferably, the application of the sweet corn seed soaking agent comprises the following steps:
firstly, diluting a sweet corn seed soaking agent;
and then, soaking the sweet corn seeds in the diluted sweet corn seed soaking agent, fishing out the sweet corn seeds after soaking, draining and sowing.
Preferably, the sweet corn seed soaking agent is diluted by 120 times compared with water.
Preferably, when the sweet corn seed soaking agent is used for soaking sweet corn seeds, the ratio of the sweet corn seed soaking agent to the sweet corn seeds is 2-3: 1.
preferably, the sweet corn seeds are soaked in the sweet corn seed soaking agent for 2-4 hours.
The invention at least comprises the following beneficial effects:
(1) the sweet corn seed soaking agent is safe for sweet corn seeds, and is harmless and residue-free in the using process;
(2) the sweet corn seed soaking agent is a sweet corn seed treating agent which is compounded by a growth regulator, a biological stimulator, nutrient elements and a bactericide in a multi-element manner, has multiple effects, and better copes with the environmental stress influence of the germination stage and the seedling stage of the sweet corn;
(3) the sweet corn seed soaking agent effectively improves the topsoil capability, the emergence rate and the growth vigor of the sweet corn, improves the capabilities of the sweet corn in germination and emergence periods such as drought resistance, waterlogging resistance, high temperature resistance, disease resistance and the like, and effectively improves the seedling-stage quality of the sweet corn;
(4) the sweet corn seed soaking agent has the advantages of simple use method, small use amount and low cost.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The sweet corn seed soaking agent of the present invention, its preparation method and application are specifically described by the following examples. These examples are merely illustrative of the invention and are not intended to be limiting.
Example 1
1. The preparation method of the sweet corn seed soaking agent comprises the following steps:
a. mixing Ca (NO)3)2Pouring fulvic acid, chitosan and water into a mixer, heating to 45 ℃, keeping the temperature, stirring and mixing for 1.5 hours, and uniformly mixing to obtain a mixed solution;
b. and d, cooling the mixed solution obtained in the step a to a temperature of 25 ℃, adding betaine, 24-epibrassinolide, spermidine and fludioxonil, and continuously stirring and mixing in a mixer for 20 minutes to obtain the sweet corn seed soaking agent.
2. Comparative test
Comprises betaine, 24-epibrassinolide, spermidine, and Ca (NO)3)2The raw materials of the fulvic acid, the chitosan, the fludioxonil and the water are prepared according to the following nine formulas in parts by weight:
formulation (1) 10: 0.01: 5: 250: 25: 30: 5: 9600;
formulation (2) 20: 0.03: 10: 500: 50: 60: 10: 9300;
formulation (3) 40: 0.05: 20: 1000: 100: 120: 20: 8700;
formulation (4) 40: 0.06: 30: 1100: 120: 150: 25: 8500;
formulation (5) 50: 0.07: 26: 1100: 110: 140: 30: 8500;
formulation (6) 60: 0.08: 30: 1100: 120: 150: 30: 8500;
formulation (7) 60: 0.10: 30: 1200: 120: 150: 30: 8500;
formulation (8) 120: 0.20: 60: 2400: 240: 300: 60: 6800;
formulation (9) 240: 0.40: 120: 4800: 480: 600: 120: 3800;
the nine raw materials are prepared according to the preparation steps of the sweet corn seed soaking agent respectively to obtain the sweet corn seed soaking agent with 9 formulas.
And (3) diluting the sweet corn seed soaking agents obtained by the 9 formulas by 120 times respectively, wherein the weight ratio of the sweet corn seed soaking agent diluent to the sweet corn seed 2: the seed is soaked for 2 hours according to the proportion of 1, the control seed is soaked for 2 hours by using the same amount of clear water, and the test variety is Rongyu sweet No. 1. And fishing out the seeds after soaking, draining, and then sowing in soil with the soil humidity of 24% (normal water content condition), wherein the sowing place is Yong Lezhen in the right river area of Bai color city. The seedling rate, plant height, main root length and root number were investigated at the four-leaf stage.
Table 1 shows the effect of different formulations of sweet corn seed soaking agents on the emergence and seedling stage of sweet corn. According to the experimental results of the formulas (1) to (9), the seedling emergence rate, the plant height, the main root length and the root number of the sweet corn are increased and then reduced along with the increase of the concentration of each component in the sweet corn seed soaking agent; the experimental results of the formulas (1) to (8) are all superior to those of a control group, and the formulas (1) to (8) prove that the sweet corn seed soaking agent has the effects of improving the emergence rate of sweet corn and increasing the plant height, the main root length and the root number, wherein the formulas (3) to (8) obviously improve the emergence rate of sweet corn, the emergence rate is higher than 92.7%, the increase of the emergence rate is large, the promotion effect on the germination and seedling stage growth of sweet corn is good, the formula (5) has the optimal promotion effect on the germination and seedling stage growth of sweet corn, the emergence rate is as high as 96.8%, the emergence rate and the root number of the sweet corn of the formula (9) are lower than those of the control group, and the formula (9) with high concentration has a inhibition effect on the growth of the sweet corn seeds.
According to experimental data, when the concentration of each component of the sweet corn seed soaking agent is low, the influence on the emergence rate and seedling stage of the sweet corn seeds is small, and when the concentration of each component of the sweet corn seed soaking agent is too high, the growth of the sweet corn seeds can be inhibited, and a large number of experiments prove that the weight parts of the sweet corn seed soaking agent are in the range of betaine (20-80 parts), 24-epibrassinolide (0.05-0.15 part), spermidine (10-50 parts), Ca (NO)3)2(500-3600 parts), fulvic acid (100-200 parts), chitosan (100-200 parts), fludioxonil (10-50 parts) and water (5820-9260 parts), preferably the sweet corn seed soaking agent comprises betaine (40-60 parts), 24-epibrassinolide (0.05-0.1 part), spermidine (20-30 parts), Ca (NO3)2(1000-1200 parts), fulvic acid (100-120 parts), chitosan (120-150 parts), fludioxonil (20-30 parts) and water (8410-8700 parts).
TABLE 1 influence of sweet corn seed soaking agent on emergence and seedling stage of sweet corn
Example 2
Composition comparison of sweet corn seed soaking agent
Sweet corn seed soaking agents prepared by 10 formulas (shown in table 2) with different components are respectively diluted by 100 times by water, and the weight ratio of the sweet corn seed soaking agent diluent to the sweet corn seed is 2: 1 for 4 hours, soaking the control seeds in clean water for 4 hours with the same quantity, and obtaining the test variety Rongyu sweet No. 1. After the soaking, the seeds are fished out and drained, and then the seeds are respectively sown in the soil with the soil humidity of 24 percent (normal water condition) and the soil with the soil humidity of 16 percent (drought condition), wherein the sowing place is Yong Lezhen in the right river area of Bai color city. The rate of emergence, plant height, main root length and root number were investigated at four leaf stage.
Table 2 shows the effect of the sweet corn seed soaking agent on the emergence and seedling stage of the sweet corn. The results show that under normal conditions and drought conditions, the sweet corn seed soaking agents with different components have the effect of improving the emergence rate and the plant height of the sweet corn; the sweet corn seed soaking agent containing the components A + B + C + D + E + F + G can obviously improve the emergence rate of sweet corn, the emergence rate can reach 96.6% under normal conditions, the plant height can reach 35.9cm, particularly under drought conditions, the sweet corn seed soaking agent has an obvious promotion effect on the emergence rate of sweet corn, the emergence rate is improved to 90.5% from 58.4%, and the emergence rate is increased by 55%. Proves that the main components of the sweet corn seed soaking agent are betaine, 24-epibrassinolide, spermidine and Ca (NO)3)2The fulvic acid, the chitosan and the fludioxonil have better promotion effects on the germination and seedling growth of the sweet corn.
TABLE 2 influence of sweet corn seed soaking agent on emergence and seedling stage of sweet corn
Note: the letters in the components respectively represent as follows, A represents betaine, and the concentration is 5 per mill; b represents 24-epibrassinolide with a concentration of 7 ppm; c represents spermidine with a concentration of 2.6 ‰; d represents Ca (NO)3)2The concentration is 11%; e represents fulvic acid with the concentration of 1.1%; f represents chitosan, and the concentration is 1.4%; g represents fludioxonil, and the concentration is 3 per mill.
Example 3
Application of sweet corn seed soaking agent (drought resistance)
The sweet corn seed soaking agent prepared by the formula (5) in the example 1 is diluted by 100 times by water, and the weight ratio of the sweet corn seed soaking agent diluent to the sweet corn seeds 2: 1 for 4 hours, soaking the control seeds in clean water for 4 hours in the same amount, wherein the test variety is Rongyu sweet No. 1. And fishing out the seeds after soaking, draining, and then sowing in soil with the soil humidity of 16% (drought condition), wherein the sowing place is Yong le town in the right river area of Bai-color city. And investigating the emergence rate, the plant height, the main root length and the root number in the four-leaf stage.
Table 3 shows the effect of the sweet corn seed soaking agent on the emergence and seedling stage of the sweet corn. The result shows that the seed soaking agent for sweet corn seeds under the drought condition can significantly improve the emergence rate of sweet corn, increase the plant height, the main root length and the root number, and has better promotion effect on drought resistance and growth of sweet corn.
TABLE 3 influence of sweet corn seed soaking agent on emergence and seedling stage of sweet corn
Example 4
Application of sweet corn seed soaking agent (waterlogging resistance)
The sweet corn seed soaking agent prepared by the formula (5) in the example 1 is diluted by 100 times by water, and the weight ratio of the sweet corn seed soaking agent diluent to the sweet corn seeds 2: 1 for 4 hours, soaking the control seeds in clean water for 4 hours with the same quantity, and obtaining the test variety Rongyu sweet No. 1. And fishing out the seeds after soaking, draining, and then sowing in soil with the soil humidity of 31% (waterlogging condition), wherein the sowing place is Yong le town in the Right river district of Bai color city. And investigating the emergence rate, the plant height, the main root length and the root number in the four-leaf stage.
Table 4 shows the effect of the sweet corn seed soaking agent on the emergence and seedling stage of the sweet corn. The result shows that the seed soaking agent for sweet corn seeds under the waterlogging condition can obviously improve the emergence rate of sweet corn, increase the plant height, the main root length and the root number, and has better promotion effect on waterlogging tolerance and growth of sweet corn.
TABLE 4 influence of sweet corn seed soaking agent on emergence and seedling stage of sweet corn
The embodiments prove that the sweet corn seed soaking agent can obviously improve the emergence rate of the sweet corn, increase the plant height, the main root length and the root number, has a better promotion effect on the germination and seedling stage growth of the sweet corn, improves the drought resistance and waterlogging resistance of the germination and seedling stage of the sweet corn, has the functions of sterilization and high temperature resistance, can effectively improve the high temperature resistance and disease resistance of the germination and seedling stage of the sweet corn, and effectively improves the seedling stage quality of the sweet corn.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the embodiments shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (10)
1. The sweet corn seed soaking agent is characterized by comprising the following components in parts by weight: betaine (20-80 parts), 24-epibrassinolide (0.05-0.15 parts), spermidine (10-50 parts), Ca (NO)3)2(500-3600 portions), fulvic acid (100-200 portions), chitosan (100-200 portions), fludioxonil (10-50 portions) and water (5820-9260 portions).
2. The sweet corn seed soak of claim 1 wherein the betaine (40-60 parts), 24-epibrassinolide (0.05-0.1 parts), spermidine (20-30 parts), Ca (NO)3)2(1000-1200 parts))120 portions of fulvic acid (100), 150 portions of chitosan (120), 20-30 portions of fludioxonil and 8700 portions of water (8410).
3. A method of making the sweet corn seed soak of claim 1, comprising the steps of:
a. mixing Ca (NO)3)2Mixing fulvic acid, chitosan and water, heating, and uniformly stirring to obtain a mixed solution;
b. and c, after the mixed solution obtained in the step a is cooled, adding betaine, 24-epibrassinolide, spermidine and fludioxonil into the mixed solution, and uniformly stirring to obtain the composition.
4. The method for preparing the sweet corn seed soaking agent according to claim 3, wherein the temperature for mixing and stirring in the step a is 40-50 ℃.
5. The method of claim 3 wherein the stirring time in step a is 1-2 hours.
6. The method of claim 3 wherein the stirring time in step b is 20-30 minutes.
7. The use of the sweet corn seed soak of claim 1 including the steps of:
firstly, diluting a sweet corn seed soaking agent;
and then, soaking the sweet corn seeds in the diluted sweet corn seed soaking agent, fishing out the sweet corn seeds after soaking, draining and sowing.
8. The use of the sweet corn seed soak of claim 7 wherein the dilution is 100-fold and 120-fold dilution with water.
9. The use of the sweet corn seed soaking agent according to claim 7, wherein the ratio of the sweet corn seed soaking agent to the sweet corn seed is 2-3: 1.
10. the use of the sweet corn steep of claim 7, wherein the sweet corn seeds are steeped in the sweet corn steep for a period of 2 to 4 hours.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110656125A (en) * | 2019-09-23 | 2020-01-07 | 四川育良生物科技有限公司 | Genetic transformation method of drought-resistant corn |
| CN115281213A (en) * | 2022-08-18 | 2022-11-04 | 广西壮族自治区农业科学院 | Sugarcane seed stem coating agent applied at normal temperature in large scale and application thereof |
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| CN115281213B (en) * | 2022-08-18 | 2024-03-29 | 广西壮族自治区农业科学院 | Sugarcane seed stem coating agent applied at normal temperature in large scale and application thereof |
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