CN114451511B - Antibacterial nano-particles and preparation method and application thereof - Google Patents

Antibacterial nano-particles and preparation method and application thereof Download PDF

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CN114451511B
CN114451511B CN202210008131.9A CN202210008131A CN114451511B CN 114451511 B CN114451511 B CN 114451511B CN 202210008131 A CN202210008131 A CN 202210008131A CN 114451511 B CN114451511 B CN 114451511B
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polyphenol
antibacterial
zein
nano particles
particles
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CN114451511A (en
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尹寿伟
曹晓璇
黄小楠
杨晓泉
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South China University of Technology SCUT
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses an antibacterial nanoparticle, and a preparation method and application thereof. The antibacterial nanoparticle comprises zein nanoparticles and a wrapped polyphenol-metal coordination compound, wherein the polyphenol-metal coordination compound is obtained by carrying out coordination reaction on polyphenol and ferric salt. The preparation method of the antibacterial nano-particles comprises the following steps: 1) Preparing zein nano particles by an anti-solvent method; 2) Adding zein nano particles into polyphenol water solution to react to obtain polyphenol modified nano particles; 3) And adding the polyphenol modified nano particles into an iron salt solution to carry out coordination reaction. The antibacterial nano particles can quickly generate high temperature through a photo-thermal effect under the irradiation of near infrared light, achieve a short-time broad-spectrum antibacterial effect, have good biocompatibility, are green and natural in preparation raw materials, are free of toxicity and harm, and have wide application prospects in the fields of food antibacterial, medical antibacterial and the like.

Description

Antibacterial nano-particles and preparation method and application thereof
Technical Field
The invention relates to the technical field of food antibiosis, in particular to antibiosis nano particles, a preparation method and application thereof.
Background
In recent decades, due to the large amount of broad-spectrum antibiotics, multi-drug resistant bacteria and high-toxic pathogenic bacteria appear, and the development speed of novel antibiotics is far behind the evolution speed of bacterial drug resistance, so that the requirement of the antibiotics used clinically at present is difficult to completely meet, and the development of novel bactericidal products is urgent.
Photothermal antibacterial is a method of using local heat generated by photothermal agent under appropriate irradiation, such as cell membrane rupture, protein/enzyme denaturation, cell cavitation and finenessA physical sterilization method for effectively killing bacteria by various heat effects such as evaporation of intracellular liquid. Noble metal (such as gold, silver, ruthenium and the like) nano-materials have excellent photo-thermal conversion efficiency and become a photo-thermal agent of great interest. Ding et al realized two-photon thermal effect and silver ion release using positively charged Au-Ag core-shell nanoparticles at wavelength 808nm, 2W/cm 2 The effect of killing 92% of staphylococcus aureus can be achieved by irradiating for 4min under the near infrared laser (NIR). However, the shape, size, etc. of the noble metal nanomaterial have a great influence on the photo-thermal properties thereof, and complicated operations are often required to regulate the shape, size, and nanostructure of the noble metal nanomaterial, the cost is relatively high, and the noble metal nanomaterial generally has long-term cytotoxicity, which severely limits the practical application of the noble metal nanomaterial.
Therefore, the development of the photothermal agent with good sterilization effect, no toxicity and good biocompatibility has very important significance.
Disclosure of Invention
The invention aims at providing an antibacterial nanoparticle, and a preparation method and application thereof.
The technical scheme adopted by the invention is as follows:
an antibacterial nanoparticle comprises zein nanoparticles and a wrapped polyphenol-metal coordination compound, wherein the polyphenol-metal coordination compound is obtained by carrying out coordination reaction on polyphenol and ferric salt.
Preferably, the polyphenol is one of tannic acid, gallic acid, epigallocatechin gallate and procyanidine.
Preferably, the ferric salt is at least one of ferric nitrate, ferric chloride and ferric sulfate.
Preferably, the particle size of the antibacterial nano-particles is 122nm to 396nm.
Preferably, the particle size of the zein nano-particles is 106 nm-220 nm.
The preparation method of the antibacterial nano-particles comprises the following steps:
1) Preparing zein nano particles by an anti-solvent method;
2) Adding zein nano particles into polyphenol water solution to react to obtain polyphenol modified nano particles;
3) And adding the polyphenol modified nano particles into ferric salt solution to carry out coordination reaction, thus obtaining the antibacterial nano particles.
Preferably, the preparation method of the antibacterial nanoparticle comprises the following steps:
1) Preparing zein nano particles by an anti-solvent method;
2) Adding zein nano particles into polyphenol water solution to react, and dialyzing in water to obtain polyphenol modified nano particles;
3) Adding polyphenol modified nano particles into ferric salt solution, carrying out coordination reaction, and dialyzing in water to obtain the antibacterial nano particles.
Preferably, the specific operation of preparing zein nanoparticles by the antisolvent method in step 1) is as follows: dissolving zein in ethanol water solution, adding water, and removing ethanol to obtain zein nanoparticle.
Further preferably, the specific operation of preparing zein nanoparticles by the antisolvent method in step 1) is as follows: dissolving zein in ethanol water solution, adding into water in a stirring state, and performing rotary evaporation to remove ethanol to obtain zein nano particles.
Preferably, the volume fraction of ethanol in the ethanol aqueous solution is 70% -90%.
Further preferably, the volume fraction of ethanol in the ethanol aqueous solution is 80% -90%.
Preferably, the addition amount ratio of the zein to the ethanol water solution is 30 mg-50 mg:1mL.
Preferably, the rotary evaporation is performed at a temperature of 42-48 ℃ and a rotation speed of 150-180 r/min.
Preferably, the mass ratio of the zein to the polyphenol is 1:0.04-0.12.
Preferably, the reaction time in the step 2) is 15-40 h.
It is further preferred that the reaction time in step 2) is 20 to 30 hours.
Preferably, the molar ratio of the polyphenol to the ferric salt is 0.2-2:1.
Preferably, the coordination reaction of step 3) is carried out under light-shielding conditions.
Preferably, the time of the coordination reaction in the step 3) is 2-10 hours.
It is further preferable that the time of the coordination reaction in step 3) is 2 to 6 hours.
The beneficial effects of the invention are as follows: the antibacterial nano particles can quickly generate high temperature through a photo-thermal effect under the irradiation of near infrared light (NIR), achieve a short-time broad-spectrum antibacterial effect, have good biocompatibility, are green and natural in preparation raw materials, are free of toxicity and harm, and have wide application prospects in the fields of food antibacterial, medical antibacterial and the like.
Specifically:
1) According to the invention, firstly, the zein nano-particles are modified by polyphenol, then chelation of polyphenol and iron ions is carried out to form a polyphenol-metal coordination compound, and the zein nano-particles are tightly wrapped by the polyphenol-metal coordination compound, so that the antibacterial nano-particles with short-time broad-spectrum antibacterial effect are obtained;
2) The antibacterial nano particles are prepared from green natural edible polyphenol, have no toxicity and good biocompatibility, and the stability and activity of the antibacterial nano particles can be improved by the polyphenol;
3) The antibacterial nano particles of the invention break thalli by utilizing the principle of spontaneous generation of high heat by absorbing infrared rays, thereby achieving the effect of high-efficiency broad-spectrum sterilization, and have stronger antibacterial property on drug-resistant bacteria as well, and can effectively inhibit the growth of the drug-resistant bacteria;
4) The preparation method of the antibacterial nano-particles is simple, the preparation conditions are mild, and the antibacterial nano-particles are suitable for large-scale production and application.
Drawings
FIG. 1 is a particle size distribution diagram and FE-SEM diagram of antibacterial nanoparticles of example 1.
Fig. 2 is a graph showing the photo-thermal response effects of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol-modified nanoparticles of comparative example.
FIG. 3 is a graph showing the photo-thermal sterilization effect of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol-modified nanoparticles of comparative example on E.coli.
Fig. 4 is a graph showing the photo-thermal sterilization effect of the antibacterial nanoparticles of examples 1 to 5 and the polyphenol-modified nanoparticles of comparative example on methicillin-resistant staphylococcus aureus.
Fig. 5 is a graph of the antimicrobial effect of the food medium of the antimicrobial nanoparticles of examples 1-3 and the polyphenol modified nanoparticles of the comparative example.
Detailed Description
The invention is further illustrated and described below in connection with specific examples.
Example 1:
an antibacterial nanoparticle, the preparation method thereof comprises the following steps:
1) Dissolving 1g of zein in 20mL of ethanol water solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) Adding aqueous dispersion of zein nanoparticles into 10mL of epigallocatechin gallate (EGCG) aqueous solution with the concentration of 4mg/mL, reacting for 24 hours at normal temperature, and dialyzing with deionized water at the temperature of 4 ℃ for 48 hours to obtain polyphenol modified nanoparticles;
3) Adding the polyphenol modified nano particles into 10mL ferric chloride solution with the concentration of 2.4mg/mL, stirring for 2 hours at room temperature in a dark place, and dialyzing with deionized water for 24 hours at the temperature of 4 ℃ to obtain the antibacterial nano particles (the particle size is 122-255 nm).
The particle size distribution diagram and the field emission scanning electron microscope (FE-SEM) diagram of the antibacterial nanoparticles of this example are shown in FIG. 1 (upper left small drawing is FE-SEM).
Particle size distribution testing: the antibacterial Nano particles are prepared into a dispersion liquid with the concentration of 1mg/mL by deionized, a proper volume is taken and placed in a cuvette, the particle size of the particles is measured by adopting a dynamic light scattering Nano particle sizer (Nano-ZS, malvern), and the measurement result is the average value of three measurements.
Field emission scanning electron microscope test: the conductive adhesive is stuck on a stainless steel conductive station, a mica sheet is fixed by the conductive adhesive, 10 mu L of antibacterial nanoparticle dispersion liquid with the concentration of 0.1mg/mL is evenly dripped on the mica sheet, natural air drying is carried out, and after metal spraying treatment, observation is carried out under a scanning electron microscope, and the accelerating voltage is 5.0kV.
As can be seen from fig. 1: the antibacterial nano particles of the embodiment are spherical with smooth surfaces and uniform sizes, the average particle size is 179.57nm plus or minus 0.33nm, and the particle sizes are intensively distributed between 122nm and 255 nm.
Example 2:
an antibacterial nanoparticle, the preparation method thereof comprises the following steps:
1) Dissolving 1g of zein in 20mL of ethanol water solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) Adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting for 24 hours at normal temperature, and dialyzing with deionized water at the temperature of 4 ℃ for 48 hours to obtain polyphenol modified nanoparticles;
3) Adding the polyphenol modified nano particles into 10mL ferric chloride solution with the concentration of 4.8mg/mL, stirring for 2h at room temperature in a dark place, and dialyzing with deionized water at the temperature of 4 ℃ for 24h to obtain the antibacterial nano particles (with the particle size of 142-295 nm).
Example 3:
an antibacterial nanoparticle, the preparation method thereof comprises the following steps:
1) Dissolving 1g of zein in 20mL of ethanol water solution (the volume fraction of ethanol is 80%), adding deionized water while stirring until zein precipitates, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) Adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 12mg/mL, reacting for 24 hours at normal temperature, and dialyzing with deionized water at 4 ℃ for 48 hours to obtain polyphenol modified nanoparticles;
3) Adding the polyphenol modified nano particles into 10mL of ferric chloride solution with the concentration of 7mg/mL, stirring for 2h at room temperature in a dark place, and dialyzing with deionized water at the temperature of 4 ℃ for 24h to obtain the antibacterial nano particles (the particle size is 190-396 nm).
Example 4:
an antibacterial nanoparticle, the preparation method thereof comprises the following steps:
1) Dissolving 1g zein in 20mL of ethanol water solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) Adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting for 24 hours at normal temperature, and dialyzing with deionized water at the temperature of 4 ℃ for 48 hours to obtain polyphenol modified nanoparticles;
3) Adding the polyphenol modified nano particles into 10mL ferric chloride solution with the concentration of 2.4mg/mL, stirring for 2 hours at room temperature in a dark place, and dialyzing with deionized water for 24 hours at the temperature of 4 ℃ to obtain the antibacterial nano particles (the particle size is 142-295 nm).
Example 5:
an antibacterial nanoparticle, the preparation method thereof comprises the following steps:
1) Dissolving 1g zein in 20mL of ethanol water solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) Adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting for 24 hours at normal temperature, and dialyzing with deionized water at the temperature of 4 ℃ for 48 hours to obtain polyphenol modified nanoparticles;
3) Adding the polyphenol modified nano particles into 10mL of ferric chloride solution with the concentration of 9.5mg/mL, stirring for 2 hours at room temperature in a dark place, and dialyzing with deionized water for 24 hours at the temperature of 4 ℃ to obtain the antibacterial nano particles (the particle size is 142-295 nm).
Comparative example:
a polyphenol modified nanoparticle, the method of making comprising the steps of:
1) Dissolving 1g zein in 20mL of ethanol water solution (the volume fraction of ethanol is 80%), adding into 50mL of deionized water in a stirring state, and performing rotary evaporation at the temperature of 45 ℃ and the rotation speed of 150r/min to remove ethanol to obtain zein nanoparticle aqueous dispersion (the concentration is 40mg/mL, and the particle size of zein nanoparticles is 106-220 nm);
2) Adding the zein nanoparticle aqueous dispersion into 10mL of EGCG aqueous solution with the concentration of 8mg/mL, reacting for 24 hours at normal temperature, and dialyzing with deionized water for 48 hours at the temperature of 4 ℃ to obtain polyphenol modified nanoparticles.
Performance test:
1) Photo-thermal response test: the antibacterial nanoparticles of examples 1 to 5 and the polyphenol-modified nanoparticles of comparative example were each prepared into a dispersion having a concentration of 4mg/mL (zein concentration) by adding water, and then 500. Mu.L of the dispersion was placed in a centrifuge tube having a volume of 5mL, and then near infrared light (wavelength 808nm, power density 1.5W/cm) 2 ) The centrifuge tube was irradiated vertically for 10min, and the temperature change of the solution was recorded by a thermal infrared imager at 60s intervals, and the result of the photothermal response test was shown in fig. 2.
As can be seen from fig. 2:
a) The temperature of the polyphenol modified nanoparticles of the comparative example is not obviously increased after the polyphenol modified nanoparticles are vertically irradiated for 10min by near infrared light, and the antibacterial nanoparticles of the examples 1-5 all have obvious temperature increase phenomenon, which indicates that the pure polyphenol modified nanoparticles cannot generate heat to increase the temperature under the irradiation of near infrared light, and the excellent photo-thermal properties of the antibacterial nanoparticles of the examples 1-5 are endowed by the coordination compound formed after the iron ions are introduced;
b) The temperatures of the antibacterial nanoparticles in examples 1 to 3 reach 42.9 ℃, 59.1 ℃ and 64.8 ℃ after the antibacterial nanoparticles are vertically irradiated by near infrared light for 10min, which shows that the addition amount of the whole coordination compound (namely polyphenol and ferric chloride) is synchronously improved on the premise that the molar ratio of polyphenol to ferric ion is consistent, the photo-thermal response performance of the system is obviously improved, and the photo-thermal effect is in direct proportion to the addition amount of the whole coordination compound in a certain range;
c) The temperatures of the antibacterial nanoparticles of example 4 and example 5 reached 52.9 ℃ and 62.0 ℃ respectively after being vertically irradiated with near infrared light for 10min, which shows that the photo-thermal performance and the sterilization effect of the particles are enhanced to a certain extent after the molar ratio of polyphenol to iron ions is reduced under the condition that the addition amount of polyphenol is consistent, and better antibacterial effect can be obtained on the basis of controlling the cost.
2) Photo-thermal sterilization effect test (plate colony count experiment):
a) Taking 5mL of Escherichia Coli (EC) culture solution cultured to logarithmic phase, centrifuging at 4000r/min for 10min, separating out bacterial mud, washing twice, and re-suspending with sterile water to obtain a concentration of 10 7 CFU/mL of bacterial suspension, 150. Mu.L of bacterial suspension is added into the equal volume of the antibacterial nano-particle aqueous dispersion of the example 1 (the concentration is 4 mg/mL), and the mixture is uniformly mixed with near infrared light (the wavelength is 808nm, and the power density is 1.5W/cm) 2 ) Standing for 10min, standing for 10min in dark environment as illumination group (marked as NIR+), adding 150 μl of bacterial suspension into equal volume of the antibacterial nanoparticle aqueous dispersion of example 1 (concentration of 4 mg/mL), mixing, standing for 20min in dark environment as non-illumination group (marked as NIR+), and using sterile water instead of blank controlAn aqueous dispersion of antimicrobial nanoparticles;
b) The same procedure was performed with methicillin-resistant staphylococcus aureus (MRSA) culture broth, and the bacterial suspension was diluted to a concentration of 10 4 CFU/mL, 100 μL is evenly coated on LB plates, the LB plates are placed in a constant temperature incubator for culturing for 24 hours at 37 ℃, observation and record are carried out, the survival amount of bacteria treated under different conditions is calculated through log10 (CFU/mL), each sample to be tested is repeated three times, the same test is carried out on the antibacterial nanoparticles of examples 2-5 and the polyphenol modified nanoparticles of comparative examples, the obtained photo-thermal sterilization effect graph of the antibacterial nanoparticles of examples 1-5 and the polyphenol modified nanoparticles of comparative examples on escherichia coli is shown in figure 3, and the photo-thermal sterilization effect graph of the antibacterial nanoparticles of examples 1-5 and the polyphenol modified nanoparticles of comparative examples on methicillin-resistant staphylococcus aureus is shown in figure 4.
As can be seen from fig. 3 and 4:
the bacteria with the effect of the polyphenol modified nanoparticles of the comparative example have no significant difference between the colony number after NIR+ treatment and the results of NIR-treatment and blank control treatment, which shows that the antibacterial effect of the polyphenol modified nanoparticles alone is not obvious, while the bacteria with the effect of the antibacterial nanoparticles of the example 1 have log of EC and MRSA after NIR+ treatment 10 The (CFU/mL) values were reduced by about 0.12 and 0.67, respectively, compared to the blank, the bacteria with antimicrobial nanoparticles of example 2 did not grow in NIR+ treated MRSA, and the EC were slightly colonised with log 10 The (CFU/mL) value was reduced to 3.56±0.36, and the bacteria with antibacterial nanoparticle action of example 3 showed no colony growth at all in both nir+treated EC and MRSA, and the bacteria with antibacterial nanoparticle action of example 4 showed no log of EC and MRSA after nir+treatment 10 The (CFU/mL) values were reduced by about 0.52 and 2.41, respectively, compared to the blank, and the bacteria with antibacterial nanoparticle effect of example 5 showed no colony growth at all in both EC and MRSA after nir+ treatment. From this, it can be seen that the antibacterial nanoparticles of examples 1 to 5 generally have better bactericidal effects against MRSA than EC, exhibiting excellent bactericidal performance against drug-resistant bacteria. In addition, under the same NIR treatment time, the sterilization effect of the antibacterial nano particles and the photothermal response temperature are positiveThe correlation fully shows that the excellent antibacterial effect of the antibacterial nano-particles is derived from the photo-thermal response performance.
3) Food matrix antibacterial effect test: taking 5mL MRSA culture solution cultured to logarithmic phase, centrifuging at 4000r/min for 10min, separating out bacterial mud, washing twice, and re-suspending with sterile water to obtain a concentration of 10 7 The bacterial suspension of CFU/mL is taken, fresh beef with the size specification of 2cm multiplied by 1cm is taken, 15 mu L of bacterial suspension and equal volume of the antibacterial nano particle aqueous dispersion of example 1 (with the concentration of 4 mg/mL) are respectively added on the surface of the fresh beef, and the bacterial suspension and the equal volume of the antibacterial nano particle aqueous dispersion are uniformly mixed, and are firstly mixed in near infrared light (with the wavelength of 808nm and the power density of 1.5W/cm 2 ) And (3) standing and incubating for 10min, standing and incubating for 10min in a dark environment to serve as an illumination group (marked as NIR+), taking fresh beef with the size of 2cm multiplied by 1cm, adding 15 mu L of bacterial suspension and equal volume of the antibacterial nanoparticle aqueous dispersion of the embodiment 1 (with the concentration of 4 mg/mL) on the surface of the fresh beef, uniformly mixing, standing and incubating for 20min in the dark environment to serve as a non-illumination group (marked as NIR-), adopting sterile water to replace the antibacterial nanoparticle aqueous dispersion in a blank control, respectively filling the beef into conical flasks containing 15mL of LB broth, placing the conical flasks in a constant temperature bath shaker for shaking and culturing for 7h at 37 ℃, taking 100 mu L of culture solution diluted by 1000 times, uniformly coating the culture solution on the LB broth agar medium, placing a flat plate in a constant temperature incubator for culturing for 24h at 37 ℃, observing, recording and counting flat plate colonies, measuring the bacterial survival rate, and repeating three times for each sample to be tested, and obtaining the antibacterial nanoparticle of the embodiment 2-3 and the polyphenol modified nanoparticle of the embodiment, and the antibacterial nanoparticle of the comparative example 1-3, and the polyphenol modified nanoparticle shown in the graph, and the antibacterial nanoparticle modified nanoparticle of the embodiment 5.
The bacterial viability was calculated as follows: bacterial viability (%) = number of plate colonies of sample treatment group/number of plate colonies of blank control group x 100%.
As can be seen from fig. 5: while the MRSA bacteria survival rate of the polyphenol modified nanoparticles of the comparative example is not reduced after treatment, the MRSA bacteria survival rate of the antimicrobial nanoparticles of examples 1-3 is respectively reduced to 31.31% + -0.64%, 0.57% + -0.20% and 0.08% + -0.06% after NIR+ treatment, which indicates that the antimicrobial nanoparticles still have good antimicrobial effect in food media, and have development potential as a green natural and high-quality short-time broad-spectrum antimicrobial agent in the food production and packaging processes.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.

Claims (4)

1. An antimicrobial nanoparticle comprising zein nanoparticles and a entrapped polyphenol-metal complex; the polyphenol-metal coordination compound is obtained by carrying out coordination reaction on polyphenol and ferric salt; the polyphenol is one of tannic acid, gallic acid, epigallocatechin gallate and procyanidine; the particle size of the antibacterial nano particles is 122 nm-396 nm; the particle size of the zein nano-particles is 106 nm-220 nm; the antibacterial nano-particles are prepared by a preparation method comprising the following steps: 1) Preparing zein nano particles by an anti-solvent method; 2) Adding zein nano particles into polyphenol water solution, reacting at normal temperature, and dialyzing with deionized water to obtain polyphenol modified nano particles; 3) Adding polyphenol modified nano particles into ferric salt solution, carrying out coordination reaction at room temperature in a dark place, and dialyzing with deionized water to obtain antibacterial nano particles; the mass ratio of the zein to the polyphenol is 1:0.04-0.12; the molar ratio of the polyphenol to the ferric salt is 0.2-2:1.
2. An antimicrobial nanoparticle according to claim 1, wherein: the ferric salt is at least one of ferric nitrate, ferric chloride and ferric sulfate.
3. An antimicrobial nanoparticle according to claim 1, wherein: the specific operation of preparing zein nano-particles by the anti-solvent method in the step 1) is as follows: dissolving zein in ethanol water solution, adding water, and removing ethanol to obtain zein nanoparticle.
4. Use of the antibacterial nanoparticle according to any one of claims 1 to 3 for the preparation of a photothermal agent.
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