CN114441236A - Sampling device and sample analyzer - Google Patents
Sampling device and sample analyzer Download PDFInfo
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- CN114441236A CN114441236A CN202011211172.5A CN202011211172A CN114441236A CN 114441236 A CN114441236 A CN 114441236A CN 202011211172 A CN202011211172 A CN 202011211172A CN 114441236 A CN114441236 A CN 114441236A
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- 238000005070 sampling Methods 0.000 title claims abstract description 241
- 239000007788 liquid Substances 0.000 claims abstract description 153
- 239000012530 fluid Substances 0.000 claims description 96
- 238000005086 pumping Methods 0.000 claims description 47
- 238000006243 chemical reaction Methods 0.000 claims description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims description 13
- 238000004140 cleaning Methods 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 239000002699 waste material Substances 0.000 claims description 6
- 239000012085 test solution Substances 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 86
- 238000012360 testing method Methods 0.000 description 5
- 230000009471 action Effects 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/02—Cleaning pipes or tubes or systems of pipes or tubes
- B08B9/027—Cleaning the internal surfaces; Removal of blockages
- B08B9/032—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing
- B08B9/035—Cleaning the internal surfaces; Removal of blockages by the mechanical action of a moving fluid, e.g. by flushing by suction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1418—Depression, aspiration
Abstract
The application relates to the technical field of biological analysis, specifically discloses a sampling device and sample analysis appearance, and sampling device includes at least: sampling subassembly and suction subassembly, sampling subassembly includes sampling needle and sampling pipeline, and suction subassembly includes: the first capacity of the first suction unit is smaller than the second capacity of the second suction unit; the sampling assembly is used for matching with the first suction unit to suck a first volume of sample liquid, or matching with the first suction unit and the second suction unit to suck a second volume of sample liquid, wherein the first volume is smaller than the first capacity, and the second volume is larger than the first capacity. By means of the mode, the condition that the actual sampling amount is a non-constant amount during small-dose or micro-sampling operation can be avoided, and the requirements for accuracy and repeatability are met.
Description
Technical Field
The present application relates to the field of biological analysis technology, and in particular, to a sampling device and a sample analyzer.
Background
At present, analytical instruments are widely applied to the fields of chemistry, life science pharmacy, environment and the like, and accuracy and repeatability are important indexes for evaluating the performance of the analytical instruments. In the prior art, a single syringe is usually selected for the analysis instrument for sampling operation.
However, in the long-term research and development process of the present application, it is found that a small-capacity injector cannot perform a large-dose sampling operation, and when a large-capacity injector is selected for a small-dose or micro-sampling operation, because the minimum measurement range of the large-capacity injector is too large, the actual sampling amount is not a constant amount, and the sampling accuracy and repeatability requirements cannot be met.
Disclosure of Invention
Based on this, this application provides a sampling device and sample analysis appearance to solve the problem that exists among the prior art.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a sampling device comprising at least: sampling subassembly and suction subassembly, sampling subassembly includes sampling needle and sampling pipeline, and suction subassembly includes: the first capacity of the first suction unit is smaller than the second capacity of the second suction unit; the sampling needle is communicated with the first suction unit through the sampling pipeline, the first suction unit is selectively communicated or not communicated with the second suction unit, the sampling assembly is used for being matched with the first suction unit to suck a first volume of sample liquid, or the sampling assembly is used for being matched with the first suction unit and the second suction unit to suck a second volume of sample liquid, wherein the first volume is smaller than the first capacity, and the second volume is larger than the first capacity.
Wherein the sampling assembly is configured to be filled with a sheath fluid, wherein, when the sampling assembly is used to collect a first volume of sample fluid, the first aspiration unit is configured to: aspirating at least a first volume of a sheath fluid to a first aspiration unit to inject the first volume of sample fluid into the sampling assembly; when the sampling assembly is used to collect a second volume of sample liquid, the first and second aspiration units are configured to: pumping at least a second volume of the sheath fluid to the first pumping unit and the second pumping unit to inject the second volume of the sample fluid into the sampling assembly.
Wherein, sampling device still includes: the first interface of the first electromagnetic three-way valve is connected with the second pumping unit, the second interface of the first electromagnetic three-way valve is connected with the first pumping unit, and the third interface of the first electromagnetic three-way valve is connected with the first liquid outlet of the sheath liquid storage tank; the sheath fluid storage tank is configured to: storing a sheath fluid; the first electromagnetic three-way valve may be selectively configured to: the second suction unit is communicated with the sheath fluid storage tank and is not communicated with the first suction unit, or the second suction unit is communicated with the first suction unit and is not communicated with the sheath fluid storage tank.
Wherein, the suction assembly still includes: the first driving unit is connected with the first suction unit and the second suction unit; the first driving unit is used for simultaneously driving the first suction unit and the second suction unit to perform quantitative suction operation so as to suck the sheath liquid to the first suction unit or a combination of the first suction unit and the second suction unit, thereby injecting the sample liquid into the sampling assembly.
Wherein, when the sampling assembly is used to collect a first volume of sample liquid, the first electromagnetic three-way valve is configured to: the second suction unit is communicated with the sheath fluid storage tank and is not communicated with the first suction unit; the first driving unit drives the first suction unit and the second suction unit to perform a suction operation such that the first suction unit is configured to: drawing at least a first volume of sheath fluid from the sampling assembly to a first drawing unit in accordance with sampling requirements, thereby injecting the first volume of sample fluid into the sampling assembly; and the second suction unit is configured to: the sheath liquid is sucked from the sheath liquid storage tank to the second suction unit.
Wherein, when the sampling assembly is used to collect a second volume of sample liquid, the first electromagnetic three-way valve is configured to: the second suction unit is communicated with the first suction unit and is not communicated with the sheath fluid storage tank; the first driving unit drives the first suction unit and the second suction unit to perform a suction operation such that the first suction unit is configured to: drawing a third volume of sheath fluid from the sampling assembly to the first drawing unit according to sampling requirements; and the second suction unit is configured to: a fourth volume of the sheath fluid is aspirated via the first aspiration unit to the second aspiration unit to inject the second volume of the sample fluid into the sampling assembly, wherein a sum of the third volume and the fourth volume matches the second volume.
Wherein, while the sampling assembly is performing the sample liquid collection operation, prior to injection of the sample liquid into the sampling assembly, the sampling assembly is further configured to: aspirating a first gas segment of a first predetermined volume to isolate sheath fluid in the sampling assembly from a sample fluid to be aspirated; after the sample fluid is injected into the sampling assembly, the sampling assembly is further configured to: sucking a second gas section of a second preset volume and discharging the second gas section of a third preset volume to eliminate a return error of the first driving unit in advance when the first driving unit subsequently performs a sample liquid discharging operation, wherein the third preset volume is smaller than the second preset volume; while the sampling assembly is performing the sample liquid discharge operation, the sampling assembly is further configured to: the predetermined volume of sample fluid and the fourth predetermined volume of the first gas section are vented to ensure that all of the sample fluid collected by the sampling assembly is vented.
Wherein, sampling device still includes: the cleaning swab comprises a liquid inlet pipeline and a liquid outlet pipeline which are respectively communicated with the sampling needle, a first interface of the second electromagnetic three-way valve is connected with the first suction unit, a second interface of the second electromagnetic three-way valve is connected with the sampling pipeline, and a third interface of the second electromagnetic three-way valve is connected with the liquid inlet pipeline; the electromagnetic three-way valve may be selectively configured to: the first suction unit is communicated with the sampling pipeline and is not communicated with the liquid inlet pipeline, or the first suction unit is communicated with the liquid inlet pipeline and is not communicated with the sampling pipeline.
Wherein, when the wash swab is used to wash the sampling needle, the first electromagnetic three-way valve is configured to: the second suction unit is communicated with the first suction unit and is not communicated with the sheath fluid storage tank; the second electromagnetic three-way valve is configured to: the first suction unit is communicated with the liquid inlet pipeline and is not communicated with the sampling pipeline; the first driving unit drives the first suction unit and the second suction unit to perform a push-out operation such that the second suction unit is configured to: pushing the sheath liquid out of the second suction unit to the first suction unit according to the cleaning requirement, and enabling the first suction unit to be configured to: and pushing the sheath liquid to the liquid inlet pipeline from the first suction unit, so that the sampling needle is cleaned, and waste liquid is discharged through the liquid outlet pipeline.
Wherein, when the sampling assembly is used to drain the first volume of sample liquid, the second electromagnetic three-way valve is configured to: the first suction unit is communicated with the sampling pipeline and is not communicated with the liquid inlet pipeline, and the first electromagnetic three-way valve is configured to: the second suction unit is communicated with the sheath fluid storage tank and is not communicated with the first suction unit, and the first driving unit drives the first suction unit and the second suction unit to perform a pushing-out operation, so that the second suction unit is configured to: pushing out the sheath liquid from the second suction unit to the sheath liquid storage tank, and causing the first suction unit to be configured to: pushing the sheath fluid out of the first pumping unit to the sampling assembly, so that the first volume of the sample fluid collected by the sampling assembly is completely discharged; alternatively, when the sampling assembly is used to drain a second volume of sample liquid, the second electromagnetic three-way valve is configured to: the first suction unit is communicated with the sampling pipeline and is not communicated with the liquid inlet pipeline, and the first electromagnetic three-way valve is configured to: the second suction unit is communicated with the first suction unit and is not communicated with the sheath fluid storage tank, and the first driving unit drives the first suction unit and the second suction unit to perform pushing-out operation, so that the second suction unit is configured to: pushing the sheath liquid from the second suction unit to the first suction unit, and causing the first suction unit to be configured to: the sheath fluid is pushed out of the first pumping unit to the sampling assembly, thereby completely draining the first volume of sample fluid collected by the sampling assembly.
In order to solve the technical problem, the application adopts a technical scheme that: there is provided a sample analyzer comprising: the sampling unit is the sampling device; a reagent unit for providing a required reaction reagent of the sample analyzer; the reaction unit is used for mixing the sample liquid collected by the sampling unit with the reaction reagent provided by the reagent unit to prepare a test solution; and the detection unit is adjacent to the reaction unit and used for detecting the test solution generated by the reaction unit to form detection information.
The beneficial effect of this application is: be different from prior art's condition, above-mentioned sampling device has set up two suction unit of different capacity, can be according to the sampling demand of difference, through the first suction unit of sampling subassembly cooperation in order to aspirate the sample liquid of first volume, perhaps, be used for cooperating first suction unit and second suction unit in order to aspirate the sample liquid of second volume through the sampling subassembly, both satisfy the sampling demand of large dose, the condition that the actual sampling volume is the inconstant volume appears when avoiding little dose or micro-sampling operation again, satisfy the accuracy and the repeatability requirement of sampling.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts. Wherein:
FIG. 1 is a block diagram schematically illustrating the structure of a sampling device according to an embodiment of the present application;
fig. 2 is a block diagram schematically illustrating a structure of a sample analyzer according to an embodiment of the present application.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in this specification in order not to obscure the core of the present application with unnecessary detail, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).
Referring to fig. 1, the present application provides a sampling device 10, where the sampling device 10 at least includes: a sampling assembly 11 and a suction assembly 12. Sampling assembly 11 comprises a sampling needle 111 and a sampling line 112, and aspiration assembly 12 comprises: a first suction unit 121 and a second suction unit 122, and a first capacity of the first suction unit 121 is smaller than a second capacity of the second suction unit 122.
The first pumping unit 121 and the second pumping unit 122 may be embodied as syringes or quantitative pumps.
In this embodiment, the first pumping unit 121 and the second pumping unit 122 may be syringes, wherein a piston rod of the syringe is connected to the driving mechanism, a syringe, i.e. a chamber portion, of the syringe, and an injection hole, i.e. an outlet portion, is formed at one end of the syringe. The injector changes the pressure in the cavity by changing the volume of the variable cavity, so that the sample liquid is sucked or discharged through the outlet part, and the injector has a simple structure and is convenient to control.
The first capacity of the first pumping unit 121 and the second capacity of the second pumping unit 122 may be set according to the requirement of taking the sample solution, for example, the first capacity of the first pumping unit 121 is 100 μ L to 500 μ L, and the first capacity of the first pumping unit 121 is 5mL to 15 mL.
The sampling needle 111 is communicated with the first suction unit 121 through the sampling pipeline 112, and the first suction unit 121 is selectively communicated or not communicated with the second suction unit 122.
Specifically, when the sampling needle 111 is communicated with the first suction unit 121 through the sampling pipeline 112, and the first suction unit 121 is not communicated with the second suction unit 122, the sampling assembly 11 is configured to cooperate with the first suction unit 121 to draw a first volume of sample liquid from a test tube (not shown), where the first volume is smaller than the first capacity.
When the sampling needle 111 is communicated with the first suction unit 121 through the sampling pipeline 112, and the first suction unit 121 is communicated with the second suction unit 122, the sampling assembly 11 is used for matching the first suction unit 121 to draw a second volume of sample liquid from the test tube, wherein the second volume is larger than the first volume and is smaller than the sum of the first volume and the second volume.
The sample liquid may be diluted or undiluted blood or other human tissue fluid.
Different from the prior art, the sampling device 10 is provided with two pumping units 121 and 122 with different capacities, and can cooperate with the first pumping unit 121 through the sampling assembly 11 to pump a first volume of sample liquid according to different sampling requirements, or cooperate with the first pumping unit 121 and the second pumping unit 122 through the sampling assembly 11 to pump a second volume of sample liquid, so that the sampling requirement of a large dosage is met, the condition that the actual sampling quantity is not a constant quantity during a small dosage or micro-sampling operation is avoided, and the sampling accuracy and repeatability requirements are met.
In one embodiment, the sampling assembly 11 needs to be initialized each time before the sampling device 10 performs a sampling operation, so that the sampling assembly 11 is configured to be filled with the sheath fluid.
The sampling needle 111 is communicated with the first suction unit 121 or the combination of the first suction unit 121 and the second suction unit 122 through the sampling pipeline 112, when sampling is needed, by increasing the volumes of the containing cavities of the first suction unit 121 or the first suction unit 121 and the second suction unit 122, at this time, the internal pressure of the first suction unit 121 or the first suction unit 121 and the second suction unit 122 is smaller than the external pressure, so that the sheath fluid inside the sampling assembly 11 is sucked into the first suction unit 121 or the first suction unit 121 and the second suction unit 122, and the sample fluid inside the test tube is sucked into the sampling needle 111 or the combination of the sampling needle 111 and the sampling pipeline 112, so as to complete the operation of taking the sample fluid.
It should be noted that the sample liquid does not enter the cavity of the first suction unit 121 or the second suction unit 122 at all times.
In particular, when the sampling assembly 11 is used for acquiring a first volume of sample liquid, the first suction unit 121 is configured to: at least a first volume of sheath fluid is pumped to the first pumping unit 121 to inject the first volume of sample fluid into the sampling assembly 11. Whereas when the sampling assembly 11 is used to collect a second volume of sample liquid, the first and second pumping units 121, 122 are configured to: at least a second volume of sheath fluid is aspirated into the first aspiration unit 121 and the second aspiration unit 122 to inject a second volume of sample fluid into the sampling assembly 11.
Through the mode, the initial state of the sampling assembly 11 is consistent when the sampling device 10 works each time, namely the sampling assembly 11 is full of sheath liquid, so that the situation that the actual sampling amount is not constant due to the fact that a large amount of gas in the sampling assembly 11 is compressed during sampling can be avoided, and the requirement for repeatability of sampling is met.
In one embodiment, the sampling device 10 further comprises: a sheath liquid storage tank 13 and a first electromagnetic three-way valve 14, wherein a first port of the first electromagnetic three-way valve 14 is connected to the second pumping unit 122, a second port of the first electromagnetic three-way valve 14 is connected to the first pumping unit 121, and a third port of the first electromagnetic three-way valve 14 is connected to the first liquid outlet of the sheath liquid storage tank 13.
The sheath fluid storage tank 13 is configured to: storing sheath fluid and in a hermetically sealed state. The sheath fluid storage tank 13 is located inside the sample analyzer, stores the sheath fluid for use in a flow chamber (not shown) of the sample analyzer or the second pumping unit 122, and when storing the sheath fluid, the entire sheath fluid storage tank 13 does not exchange air with the outside, and is in a sealed state.
The first electromagnetic three-way valve 14 may be selectively configured to: the second suction unit 122 is communicated with the sheath fluid storage tank 13 without communicating the second suction unit 122 with the first suction unit 121, or the second suction unit 122 is communicated with the first suction unit 121 without communicating the second suction unit 122 with the sheath fluid storage tank 13.
Specifically, the normally closed end of the first electromagnetic three-way valve 14 is connected to the liquid outlet end of the first suction unit 121, the common end of the first electromagnetic three-way valve 14 is connected to the liquid inlet end of the second suction unit 122, and the normally open end of the first electromagnetic three-way valve 14 is connected to the sheath liquid storage tank 13.
When the sampling assembly 11 is used to collect a first volume of sample liquid, the controller controls the first electromagnetic three-way valve 14 to be powered on, so that the second suction unit 122 is communicated with the sheath liquid storage tank 13 and the second suction unit 122 is not communicated with the first suction unit 121, at this time, under the action of the second suction unit 122, a negative pressure is established in the sheath liquid storage tank 13, and under the action of the negative pressure, the sheath liquid in the sheath liquid storage tank 13 is sucked into the second suction unit 122.
When the sampling assembly 11 is used to collect a second volume of sample liquid, the controller controls the first electromagnetic three-way valve 14 to be powered on, so that the second suction unit 122 is communicated with the first suction unit 121 and the second suction unit 122 is not communicated with the sheath liquid storage tank 13, at this time, under the action of the first suction unit 121, the sheath liquid inside the sampling assembly 11 is sucked into the first suction unit 121, and the sheath liquid injected into the first suction unit 121 is sucked into the second suction unit 122.
In one embodiment, the suction assembly 12 further comprises: the first driving unit 123, the first suction unit 121, and the second suction unit 122 are connected to the first driving unit 123.
The first driving unit 123 is configured to simultaneously drive the first suction unit 121 and the second suction unit 122 to perform a quantitative suction operation to suck the sheath liquid to the first suction unit 121 or a combination of the first suction unit 121 and the second suction unit 122, so as to inject the sample liquid into the sampling assembly 11.
Specifically, when the first suction unit 121 and the second suction unit 122 are syringes, the first driving unit 123 is a stepping motor, the first suction unit 121 and the second suction unit 122 are both connected to the same stepping motor, the stepping motor drives a lead screw, and the lead screw pushes piston rods of the first suction unit 121 and the second suction unit 122 to move, so that a power source for sucking sheath fluid or pushing out sample fluid can be generated in the first suction unit 121 and the second suction unit 122, that is, the suction assembly 12 adopts a "one-to-two" structure, so that the structure of the sampling device 10 can be simplified, not only is the cost saved, but also the sampling device 10 is facilitated to be miniaturized.
It should be noted that the stroke of the screw rod driven by each step of the stepping motor is a fixed value, the inner hole surfaces of the first suction unit 121 and the second suction unit 122 are cylindrical surfaces with extremely high processing precision, and the sectional areas are fixed, so that the liquid amount sucked or pushed by the first suction unit 121 and the second suction unit 122 is also a standard value which can be calculated, and the precision can reach 0.001 μ L. The amounts of liquid discharged or sucked by the first suction unit 121 and the second suction unit 122 per step of the stepping motor can be calculated by combining the step pitch of the stepping motor, the capacities of the first suction unit 121 and the second suction unit 122, and the inner diameters of the first suction unit 121 and the second suction unit 122.
Specifically, when the sampling assembly 11 is used to collect a first volume of sample liquid, the first electromagnetic three-way valve 14 is configured to: the second suction unit 122 is communicated with the sheath fluid storage tank 13 and the second suction unit 122 is not communicated with the first suction unit 121. At this time, the first driving unit 123 drives the first suction unit 121 and the second suction unit 122 to perform a suction operation such that the first suction unit 121 is configured to: drawing at least a first volume of sheath fluid from the sampling assembly 11 to the first drawing unit 121 in accordance with a first sampling requirement, thereby injecting the first volume of sample fluid into the sampling assembly 11; and the second pumping unit 122 is configured to: the sheath liquid is sucked from the sheath liquid storage tank 13 into the second suction unit 122.
And when the sampling assembly 11 is used to collect a second volume of sample liquid, the first electromagnetic three-way valve 14 is configured to: the second suction unit 122 is communicated with the first suction unit 121 and the second suction unit 122 is not communicated with the sheath fluid storage tank 13. At this time, the first driving unit 123 drives the first suction unit 121 and the second suction unit 122 to perform a suction operation such that the first suction unit 121 is configured to: drawing a third volume of sheath fluid from the sampling assembly 11 to the first drawing unit 121 in accordance with a second sampling requirement; and the second pumping unit 122 is configured to: a fourth volume of sheath fluid is pumped via the first pumping unit 121 to the second pumping unit 122, thereby injecting a second volume of sample fluid into the sampling assembly 11, wherein the sum of the third volume and the fourth volume matches the second volume.
Because the drive part of the injector inevitably has return stroke errors, the return stroke errors can directly influence the accuracy and the repeatability of trace sample adding, thereby influencing the precision of the sample adding device. To solve this technical problem, in one embodiment, when the sampling assembly 11 performs a sample liquid collection operation, before the sample liquid is injected into the sampling assembly 11, the sampling assembly 11 of the present application is further configured to: a first gas segment of a first predetermined volume is aspirated to isolate the sheath fluid in the sampling assembly 11 from the sample fluid to be aspirated. After the sample fluid is injected into the sampling assembly 11, the sampling assembly 11 is further configured to: the second gas section of the second preset volume is sucked and the second gas section of the third preset volume, which is smaller than the second preset volume, is discharged to previously eliminate a return error of the first driving unit 123 in subsequently performing the sample liquid discharging operation.
Specifically, when the sampling device 10 performs a sample liquid collection operation, before the sampling assembly 11 contacts the liquid surface of the sample liquid in the test tube, the first driving unit 123 moves in a direction away from the injection holes (outlet portions) of the first suction unit 121 and the second suction unit 122, at this time, the volumes of the first suction unit 121 and the second suction unit 122 increase, the pressure in the chamber portion decreases, and the atmospheric pressure is higher than the pressure in the chamber portion, so that air in the atmosphere is pressed into the sampling needle 111, and a first gas segment for isolating the sheath liquid and the sample liquid is formed at the tip portion of the sampling needle 111. Then, the sampling assembly 11 continues to move below the liquid level of the sample liquid, and the driving mechanism can continue to move away from the injection hole (outlet portion), so that the sample liquid is sucked into the sampling needle 111; after the sample liquid collection operation is finished, the sampling needle 111 leaves the liquid level of the sample liquid in the test tube, and meanwhile, the driving mechanism can continue to move towards the direction far away from the injection hole (outlet part), so that the needle tip part of the sampling needle 111 forms a second gas section; a second gas segment is formed at the needle tip portion of the sampling needle 111, and the driving mechanism can move in a direction close to the injection hole (outlet portion) so that the sampling needle 111 discharges the second gas segment to eliminate in advance a return error of the first driving unit 123 subsequently performing the sample liquid discharging operation. And the third preset volume is smaller than the second preset volume, and the difference value between the third preset volume and the second preset volume is equal to the return error. Meanwhile, the second gas section can prevent the sampling needle 111 from throwing out the sample liquid at the needle tip part in the moving process.
In one embodiment, when the sampling assembly 11 performs a sample fluid discharge operation, the sampling assembly 11 is further configured to: the predetermined volume of sample liquid and the fourth predetermined volume of the first gas section are evacuated to ensure that the sample liquid collected by the sampling assembly 11 is completely evacuated.
Specifically, by reducing the volumes of the chamber parts of the first and second pumping units 121 and 122, a predetermined volume of the sample liquid and a fourth preset volume of the first gas section are discharged. For example, when the first suction unit 121 and the second suction unit 122 are syringes, a pushing force is applied to a piston rod of the syringe, so that the sheath liquid contained in the first suction unit 121 is injected into the first suction unit 121 or the sheath liquid storage tank 13, and the sheath liquid contained in the first suction unit 121 is injected into the sampling assembly 11, so as to ensure that the sample liquid collected by the sampling assembly 11 is completely discharged, thereby completing the sample discharge operation.
In one embodiment, the sampling device 10 further comprises: the cleaning swab 15 comprises a liquid inlet pipeline 151 and a liquid outlet pipeline 152, the liquid inlet pipeline 151 and the liquid outlet pipeline 152 are respectively communicated with the sampling needle 111, a first interface of the second electromagnetic three-way valve 16 is connected with the first suction unit 121, a second interface of the second electromagnetic three-way valve 16 is connected with the sampling pipeline 112, and a third interface of the second electromagnetic three-way valve 16 is connected with the liquid inlet pipeline 151; the electromagnetic three-way valve may be selectively configured to: the first pumping unit 121 and the sampling pipeline 112 are communicated without communicating the first pumping unit 121 and the liquid inlet pipeline 151, or the first pumping unit 121 and the liquid inlet pipeline 151 are communicated without communicating the first pumping unit 121 and the sampling pipeline 112.
Wherein, when the washing swab 15 is used for washing the sampling needle 111, the first electromagnetic three-way valve 14 is configured to: the second suction unit 122 and the first suction unit 121 are communicated and the second suction unit 122 and the sheath fluid storage tank 13 are not communicated; the second electromagnetic three-way valve 16 is configured to: the first suction unit 121 is communicated with the liquid inlet pipeline 151 and the first suction unit 121 is not communicated with the sampling pipeline 112; the first driving unit 123 drives the first suction unit 121 and the second suction unit 122 to perform a push-out operation, so that the second suction unit 122 is configured to: the sheath liquid is pushed out from the second suction unit 122 to the first suction unit 121 according to the cleaning requirement, and the first suction unit 121 is configured to: the sheath liquid is pushed out from the first pumping unit 121 to the liquid inlet line 151, thereby cleaning the sampling needle 111, and the waste liquid is discharged through the liquid outlet line 152.
The third driving unit 17 is respectively communicated with the liquid outlet pipe 152 and the waste liquid connecting pipe assembly 18, and the third driving unit 17 is used for sucking the waste liquid for cleaning the swab 15 to the waste liquid connecting pipe assembly 18.
Wherein, when the sampling assembly 11 is used for discharging the first volume of sample liquid, the second electromagnetic three-way valve 16 is configured to: the first pumping unit 121 and the sampling line 112 are communicated and the first pumping unit 121 and the liquid inlet line 151 are not communicated, and the first electromagnetic three-way valve 14 is configured to: the second suction unit 122 is communicated with the sheath fluid storage tank 13 and the second suction unit 122 is not communicated with the first suction unit 121, and the first driving unit 123 drives the first suction unit 121 and the second suction unit 122 to perform the pushing-out operation, so that the second suction unit 122 is configured to: the sheath liquid is pushed out from the second suction unit 122 to the sheath liquid storage tank 13, and the first suction unit 121 is configured to: pushing the sheath liquid out of the first suction unit 121 to the sampling assembly 11, so that the first volume of the sample liquid collected by the sampling assembly 11 is completely discharged; alternatively, when the sampling assembly 11 is used to expel a second volume of sample liquid, the second electromagnetic three-way valve 16 is configured to: the first pumping unit 121 and the sampling line 112 are communicated and the first pumping unit 121 and the liquid inlet line 151 are not communicated, and the first electromagnetic three-way valve 14 is configured to: the second suction unit 122 is communicated with the first suction unit 121 and the second suction unit 122 is not communicated with the sheath liquid storage tank 13, and the first driving unit 123 drives the first suction unit 121 and the second suction unit 122 to perform the pushing-out operation, so that the second suction unit 122 is configured to: the sheath liquid is pushed out from the second suction unit 122 to the first suction unit 121, and the first suction unit 121 is configured to: the sheath fluid is pushed out of the first pumping unit 121 to the sampling assembly 11, so that the first volume of the sample fluid collected by the sampling assembly 11 is completely discharged.
Referring to fig. 2, the present application further provides a sample analyzer 100, wherein the sample analyzer 100 may be a blood cell analyzer or an immunoassay analyzer. Wherein the sample analyzer 100 includes: a sampling unit 101, a reagent unit 102, a reaction unit 103, and a detection unit 104.
The sampling unit 101 may be the sampling device 10 in any of the above embodiments. The reagent unit 102 is used to provide the required reaction reagents of the sample analyzer 100; the reaction unit 103 is used for mixing the sample solution collected by the sampling unit 101 with the reaction reagent provided by the reagent unit 102 to prepare a test solution. The detection unit 104 is adjacent to the reaction unit 103, and detects the sample solution generated by the reaction unit 103 to form detection information.
It will be appreciated that the sample analyzer 100 includes the sampling device 10, and thus has all the benefits of the sampling device 10, and will not be described in detail herein.
The above embodiments are merely examples and are not intended to limit the scope of the present disclosure, and all modifications, equivalents, and flow charts using the contents of the specification and drawings of the present disclosure or those directly or indirectly applied to other related technical fields are intended to be included in the scope of the present disclosure.
Claims (11)
1. A sampling device, characterized in that it comprises at least: sampling subassembly and suction subassembly, the sampling subassembly includes sampling needle and sampling pipeline, the suction subassembly includes: the first suction unit and the second suction unit, and the first capacity of the first suction unit is smaller than the second capacity of the second suction unit;
wherein the sampling needle is communicated with the first suction unit through the sampling pipeline, the first suction unit is selectively communicated or not communicated with the second suction unit, the sampling assembly is used for being matched with the first suction unit to suck a first volume of sample liquid, or the sampling assembly is used for being matched with the first suction unit and the second suction unit to suck a second volume of sample liquid, wherein the first volume is smaller than the first capacity, and the second volume is larger than the first capacity.
2. The sampling device of claim 1, wherein the sampling assembly is configured to be filled with a sheath fluid, wherein, when the sampling assembly is used to collect the first volume of the sample fluid, the first pumping unit is configured to: aspirating at least the first volume of the sheath fluid to the first aspiration unit to inject the first volume of the sample fluid into the sampling assembly;
when the sampling assembly is used to collect the second volume of the sample liquid, the first and second aspiration units are configured to: aspirating at least the second volume of the sheath fluid to the first aspiration unit and the second aspiration unit to inject the second volume of the sample fluid into the sampling assembly.
3. The sampling device of claim 2, further comprising: the first interface of the first electromagnetic three-way valve is connected with the second pumping unit, the second interface of the first electromagnetic three-way valve is connected with the first pumping unit, and the third interface of the first electromagnetic three-way valve is connected with the first liquid outlet of the sheath liquid storage tank;
the sheath fluid storage tank is configured to: the sheath fluid is stored;
the first electromagnetic three-way valve may be selectively configured to: the second suction unit is communicated with the sheath fluid storage tank and is not communicated with the second suction unit and the first suction unit, or the second suction unit is communicated with the first suction unit and is not communicated with the second suction unit and the sheath fluid storage tank.
4. The sampling device of claim 3, wherein the aspiration assembly further comprises: the first driving unit is connected with the first suction unit and the second suction unit;
the first driving unit is used for simultaneously driving the first suction unit and the second suction unit to perform quantitative suction operation so as to suck the sheath liquid to the first suction unit or the combination of the first suction unit and the second suction unit, thereby injecting the sample liquid into the sampling assembly.
5. The sampling device of claim 4, wherein, when the sampling assembly is used to collect the first volume of the sample fluid,
the first electromagnetic three-way valve is configured to: communicating the second suction unit with the sheath fluid storage tank and not communicating the second suction unit with the first suction unit;
the first driving unit drives the first suction unit and the second suction unit to perform a suction operation such that the first suction unit is configured to: aspirating at least the first volume of the sheath fluid from the sampling assembly to the first aspiration unit in accordance with sampling requirements, thereby causing the first volume of the sample fluid to be injected into the sampling assembly; and the second suction unit is configured to: pumping the sheath fluid from the sheath fluid storage tank into the second pumping unit.
6. The sampling device of claim 4, wherein, when the sampling assembly is used to collect the second volume of the sample liquid,
the first electromagnetic three-way valve is configured to: communicating the second suction unit with the first suction unit and not communicating the second suction unit with the sheath fluid storage tank;
the first driving unit drives the first suction unit and the second suction unit to perform a suction operation such that the first suction unit is configured to: drawing a third volume of the sheath fluid from the sampling assembly to the first drawing unit according to sampling requirements; and the second suction unit is configured to: aspirating a fourth volume of the sheath fluid via the first aspiration unit to the second aspiration unit to thereby inject the second volume of the sample fluid into the sampling assembly, wherein a sum of the third volume and the fourth volume matches the second volume.
7. The sampling device of any one of claims 5-6,
when the sampling assembly performs a sample fluid collection operation,
prior to the sample fluid being injected into the sampling assembly, the sampling assembly is further configured to: aspirating a first gas segment of a first preset volume to isolate the sheath fluid in the sampling assembly from the sample fluid ready for aspiration injection;
after the sample fluid is injected into the sampling assembly, the sampling assembly is further configured to: aspirating a second gas segment of a second preset volume and discharging the second gas segment of a third preset volume to eliminate in advance a return error of the first driving unit in subsequently performing a sample liquid discharging operation, wherein the third preset volume is smaller than the second preset volume;
while the sampling assembly is performing a sample liquid discharge operation, the sampling assembly is further configured to: -evacuating a predetermined volume of said sample liquid and a fourth predetermined volume of said first gas segment to ensure that all of said sample liquid collected by said sampling assembly is evacuated.
8. The sampling device of claim 4, further comprising: the cleaning swab comprises a liquid inlet pipeline and a liquid outlet pipeline, the liquid inlet pipeline and the liquid outlet pipeline are respectively communicated with the sampling needle, a first interface of the second electromagnetic three-way valve is connected with the first suction unit, a second interface of the second electromagnetic three-way valve is connected with the sampling pipeline, and a third interface of the second electromagnetic three-way valve is connected with the liquid inlet pipeline;
the electromagnetic three-way valve may be selectively configured to: the first suction unit is communicated with the sampling pipeline and is not communicated with the first suction unit and the liquid inlet pipeline, or the first suction unit is communicated with the liquid inlet pipeline and is not communicated with the first suction unit and the sampling pipeline.
9. The sampling device of claim 8, wherein when said cleaning swab is used to clean said sampling needle,
the first electromagnetic three-way valve is configured to: communicating the second suction unit with the first suction unit and not communicating the second suction unit with the sheath fluid storage tank;
the second electromagnetic three-way valve is configured to: the first suction unit is communicated with the liquid inlet pipeline and is not communicated with the sampling pipeline;
the first driving unit drives the first suction unit and the second suction unit to perform a push-out operation such that the second suction unit is configured to: pushing the sheath fluid out of the second pumping unit to the first pumping unit according to a cleaning requirement, and configuring the first pumping unit to: and pushing the sheath liquid out of the first suction unit to the liquid inlet pipeline, so that the sampling needle is cleaned, and waste liquid is discharged through the liquid outlet pipeline.
10. The sampling device of claim 8,
when the sampling assembly is used to drain the first volume of the sample liquid, the second electromagnetic three-way valve is configured to: the first suction unit is communicated with the sampling pipeline and is not communicated with the liquid inlet pipeline, and the first electromagnetic three-way valve is configured to: the second suction unit and the sheath fluid storage tank are communicated and the second suction unit and the first suction unit are not communicated, the first driving unit drives the first suction unit and the second suction unit to perform a pushing-out operation, so that the second suction unit is configured to: pushing out the sheath liquid from the second suction unit to the sheath liquid storage tank, and causing the first suction unit to be configured to: advancing the sheath fluid from the first aspiration unit to the sampling assembly such that the first volume of the sample fluid collected by the sampling assembly is expelled in its entirety; alternatively, the first and second electrodes may be,
when the sampling assembly is used to drain the second volume of the sample liquid, the second electromagnetic three-way valve is configured to: the first suction unit is communicated with the sampling pipeline and is not communicated with the liquid inlet pipeline, and the first electromagnetic three-way valve is configured to: the second suction unit and the first suction unit are communicated and the second suction unit and the sheath fluid storage tank are not communicated, the first driving unit drives the first suction unit and the second suction unit to perform a pushing-out operation, so that the second suction unit is configured to: pushing out the sheath liquid from the second suction unit to the first suction unit, and causing the first suction unit to be configured to: advancing the sheath fluid from the first aspiration unit to the sampling assembly such that the first volume of the sample fluid collected by the sampling assembly is expelled in its entirety.
11. A sample analyzer, comprising:
a sampling unit being the sampling device according to any one of claims 1-10;
a reagent unit for providing a required reaction reagent of the sample analyzer;
the reaction unit is used for mixing the sample liquid collected by the sampling unit with the reaction reagent provided by the reagent unit to prepare a test solution;
and the detection unit is adjacent to the reaction unit and is used for detecting the test solution generated by the reaction unit to form detection information.
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| CN202011211172.5A CN114441236A (en) | 2020-11-03 | 2020-11-03 | Sampling device and sample analyzer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011211172.5A CN114441236A (en) | 2020-11-03 | 2020-11-03 | Sampling device and sample analyzer |
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| CN114441236A true CN114441236A (en) | 2022-05-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117367885A (en) * | 2023-12-07 | 2024-01-09 | 江苏希诚新材料科技有限公司 | Carbon nano conductive paste viscosity testing device |
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