CN114410542B - Vibrio paradise and application thereof in degradation of enteromorpha - Google Patents
Vibrio paradise and application thereof in degradation of enteromorpha Download PDFInfo
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Abstract
The invention discloses a strain of Vibrio azurin and application thereof in degrading enteromorpha, the strain is separated from a water sample containing rotten enteromorpha by adopting a high-salt culture medium and screened by degradation effect verification to obtain Vibrio azurin (Vibrio azureus) HT-10 with good enteromorpha degradation effect, and the strain is preserved in China general microbiological culture collection center (CGMCC) No.23443 in 9 and 18 days of 2021. The bacterial colony of the strain is round on NA culture medium containing 3.5% NaCl, the surface is moist and smooth, and the edge is neat and colorless and semitransparent. The strain can directly act on the enteromorpha and efficiently degrade the enteromorpha, is easy to ferment and produce, and can replace the conventional complex treatment mode at present; the method for degrading enteromorpha by adopting the strain is simple, convenient and feasible, and is suitable for large-area popularization.
Description
Technical Field
The invention relates to Vibrio azurii and application thereof in degrading enteromorpha, belonging to the technical field of microorganisms.
Background
Enteromorpha prolifera (Enteromorpha prolifera) belongs to an algae plant of Ulvaceae and Enteromorpha, is a large filamentous economic algae which is very abundant in marine wild plants in China, and has important development value. In recent years, enteromorpha is propagated in an explosive manner due to a plurality of factors such as seawater eutrophication, marine water environment warming and the like, so that a disastrous green tide is formed; the accumulation of a large amount of enteromorpha seriously affects the marine ecological environment and the development of offshore tourism industry, and also brings great loss to the mariculture industry. At present, the most common method for treating enteromorpha is concentrated stacking and simple burying, and a large amount of sewage and malodor can be generated in the anaerobic fermentation process, so that the surrounding environment is seriously influenced. Therefore, how to rapidly treat enteromorpha becomes a current urgent problem to be solved, and also becomes a technical problem.
Under the large background, people gradually turn the eyes to utilize enteromorpha by screening microorganism degradation bacteria to recycle. Because more than 70% of the enteromorpha is crude polysaccharide, people mainly focus on how to utilize the enteromorpha crude polysaccharide single substance, neglect to additionally contain about 13% of crude protein, about 15% of ash, 1% of crude fat and the like, and cannot comprehensively utilize the enteromorpha, so that resource waste is caused; meanwhile, the research on the enteromorpha crude polysaccharide is mainly focused on degrading the crude polysaccharide into reducing sugar by utilizing microbial polysaccharide degrading enzyme, and the method is used for researching biological energy, and the pretreatment process of the enteromorpha is complex, and the produced enzyme has high cost and is not suitable for large-area popularization. Therefore, the high-efficiency degradation bacteria which can directly act on the enteromorpha are screened, and the enteromorpha degradation products are comprehensively utilized to become an optimal utilization mode of the enteromorpha.
However, the research on the enteromorpha high-efficiency degrading bacteria is less at present. Patent CN 113293114A discloses alteromonas HT1, a culture method and application thereof, and a microorganism preservation number of the alteromonas HT1 is CCTCC No. M2021217, the strain can grow in a culture medium taking enteromorpha as a sole carbon source, and a product obtained by degrading enteromorpha polysaccharide is enteromorpha oligosaccharide which mainly exists in the forms of disaccharide, trisaccharide, tetrasaccharide and pentasaccharide. Patent CN 103740597A discloses a penicillium oxalicum strain, a screening method and application thereof, the microorganism preservation number is CGMCC NO.8270, the strain has good degradation effect on cellulose substances in enteromorpha, and the yield of reducing sugar is measured to be 8.22%. Patent CN108753643a discloses a vibrio H11 capable of efficiently hydrolyzing enteromorpha polysaccharide, and the microorganism preservation number is cctccc NO: m2018172, the reducing sugar obtained by hydrolysis of which can be utilized by energy strains to produce bioenergy substances. The method has the advantages that the strain types are few in the aspect of the utilization of the enteromorpha high-efficiency degrading bacteria, the method is only used for degrading enteromorpha polysaccharide, and no report on degrading enteromorpha by vibrio azurii is found.
Disclosure of Invention
Aiming at the problems, the invention provides the Vibrio paradise for efficiently degrading the enteromorpha and the application thereof, wherein the strain is Vibrio paradise (Vibrio azureus) HT-10, and the Vibrio paradise can be efficiently degraded by combining hydrolysis and anaerobic fermentation of the Vibrio paradise, so that the strain has a wide application prospect in the development and utilization of enteromorpha resources.
The above object of the present invention is achieved by the following technical solutions: the invention adopts a high-salt culture medium to separate and screen out Vibrio azurii (Vibrio azureus) HT-10 with good enteromorpha degradation effect from a water sample containing rotten enteromorpha, and the strain is preserved in China general microbiological culture Collection center, preservation unit address, north Chen West Lu No. 1, no. 3 in the Korean region of Beijing city in 2021, 9 months and 18 days, and the preservation number is CGMCC No.23443. The bacterial colony of the strain is round on NA culture medium containing 3.5% NaCl, the surface is moist and smooth, and the edge is neat and colorless and semitransparent.
The invention also provides a fermentation method of the Vibrio azurelus (Vibrio azurelus) HT-10, which is characterized in that,
1) Culturing in NB medium containing 3.5% NaCl at 20+ -2deg.C for 24+ -12 h to obtain seed solution of Vibrio coerulescens HT-10;
2) Inoculating the seed solution of the Vibrio coerulescens HT-10 into a fermentation culture medium, and fermenting and culturing for 24+/-12 hours at 20+/-2 ℃ to obtain the fermentation solution of the Vibrio coerulescens HT-10.
NB medium containing 3.5% NaCl was: 10g of peptone, 3g of beef powder, 35g of NaCl and 1000mL of water, and the pH value is 7.3+/-0.1.
The fermentation medium (1000 ml) was: 20g of glucose, 20g of starch, 15g of yeast extract powder, 4g of fish peptone, 0.5g of magnesium sulfate, 0.5g of monopotassium phosphate, 6g of calcium carbonate and the balance of seawater, wherein the pH value is 7.5+/-0.1.
The invention further aims to provide an application of Vibrio coelicolor (Vibrio azureus) HT-10 in degrading enteromorpha.
The degraded enteromorpha is the dry matter, crude polysaccharide, crude protein and crude fat of degraded enteromorpha.
The invention also provides a method for degrading enteromorpha by utilizing Vibrio azurius HT-10, which is characterized in that Vibrio azurius HT-10 fermentation liquor is directly put into fresh enteromorpha, anaerobic fermentation is carried out at 15-25 ℃ under a sealed condition, and after 8-15 days, macromolecular crude polysaccharide, crude protein and crude fat are degraded into micromolecular glucose, amino acid, polypeptide and fatty acid, and degradation products can be directly used for feed production.
The invention has the technical effects that:
1. according to the invention, a high-salt culture medium is adopted to separate from a water sample containing rotten enteromorpha, and Vibrio azurii (Vibrio azurius) HT-10 with good enteromorpha degradation effect is screened through degradation effect verification, after enteromorpha is treated by a microbial inoculum of the strain, enteromorpha dry matter is reduced by 30.1%, polysaccharide matter is reduced by 13.5%, crude protein is reduced by 9.1%, and fat is reduced by 2.7%; the strain can directly act on enteromorpha and efficiently degrade the enteromorpha.
2. Vibrio coerulescens HT-10 is easy to ferment and produce, and the degraded enteromorpha product can be directly used for feed production; the mode of directly acting on enteromorpha can replace the conventional complex treatment mode at present; the method is simple and easy to implement, and is suitable for large-area popularization; the strain has wide application prospect in development and utilization of enteromorpha resources.
Drawings
FIG. 1 is a colony morphology of Vibrio coelicolor HT-10;
FIG. 2 shows the change in appearance state of Enteromorpha prolifera (in vitro) after different days of treatment with Vibrio coelicolor HT-10 microbial inoculum;
FIG. 3 shows the change in appearance (in a sealed bag) of Enteromorpha prolifera 9d treated with Vibrio coerulescens HT-10 microbial inoculum.
Detailed Description
The following description of the embodiments of the present invention will clearly and fully describe the technical solutions of the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1: isolation and identification of strains
1. Isolation of Strain HT-10
The water sample containing the rotten enteromorpha is collected from the yellow island region seawater bath in Qingdao, shandong, and is separated on an NA culture medium (high-salt culture medium) containing 3.5% NaCl by a dilution coating method. The specific method comprises the following steps:
(1) Sterilizing the articles: sterilizing the culture dish, gun head, sterile water and other experimental articles under high pressure at 121 ℃ under 0.1MPa for 30min for later use;
(2) Obtaining a fungus sample: mixing a water sample with the smashed and rotted enteromorpha in a ratio of 9:1, placing the mixture in a sterile triangular flask containing glass beads, oscillating for 30min under the condition of 120r/min, and filtering by four layers of sterile gauze to obtain a fungus sample;
(3) Dilution of the bacterial sample: 6 test tubes containing 9mL of sterile water are taken and numbered 10 in sequence -1 ～10 -6 The method comprises the steps of carrying out a first treatment on the surface of the 1mL of the bacterial sample is sucked from the triangular flask and put into a label 10 -1 In a test tube, namely 10 -1 Dilution; and then marked with 10 -1 1mL of the diluted bacterial liquid is put into a container marked with 10 -2 In a test tube, namely 10 -2 Dilution; sequentially 10 are prepared by the same method -3 、10 -4 、10 -5 、10 -6 A diluted bacterial sample;
(4) Preparation of the culture Medium
Isolation medium (high salt medium), i.e. NA medium containing 3.5% nacl: 10g of peptone, 3g of beef powder, 35g of NaCl, 15g of agar, 1000mL of water and pH value of 7.3+/-0.1;
purifying the culture medium and separating the culture medium;
(5) Coating: will dilute the bacterial sample 10 -3 、10 -4 、10 -5 、10 -6 Respectively coating on a separation culture medium plate, each gradient is 3 dishes, and marking the bottoms of the dishes;
(6) Culturing: after coating, culturing for about 3d at constant temperature and 20 ℃ in an inverted way, and observing;
(8) Strain purification: picking single colony by using an inoculating hook, streaking on a plate containing a purification culture medium, and culturing for 3d under the constant temperature of 20 ℃ in an inverted manner, so as to pick single bacteria.
As a result, 30 strains of bacteria were isolated in total from the high-salt medium, and HT-01 to HT-30 were numbered, respectively.
2. Screening of Strain HT-10
Culturing the separated strains for 24 hours in a shaking flask to prepare shaking flask microbial inoculum of different strains (see example 2) for later use; weighing 5g of fresh enteromorpha, and placing in a test tube, wherein the compactness is kept consistent; adding 1mL of different strain shaking bottle bactericides into each test tube, plugging the plug, and providing an anaerobic environment; after 5d at 20℃the observation was carried out.
The result shows that enteromorpha inoculated with HT-10 microbial inoculum starts to turn black after 5d of treatment; the enteromorpha inoculated with the HT-07 microbial inoculum turns brown and has serious hydration; the color of the enteromorpha inoculated with HT-05, HT-08, HT-15, HT-18 and HT-22 bacteria is lightened from green. The results above initially demonstrate that HT-05, HT-07, HT-08, HT-10, HT-15, HT-18 and HT-22 have enteromorpha degrading function and that HT-10 and HT-07 are superior to other strains.
TABLE 1 summary of degradation of Enteromorpha by different strains
The criteria for judging the activity level were: the black development of the enteromorpha is changed to a "++ +" level, the browning of the enteromorpha is changed to a "++" level, the lightening of the enteromorpha from green is changed to a "+" level, and the non-discoloration of the enteromorpha is changed to a "-" level.
3. Morphological and molecular biological identification of strain HT-10
(1) Morphological characteristics: bacterial strain HT-10 was inoculated on NA medium containing 3.5% NaCl and incubated at 20℃for 24h to observe colony characteristics. As a result, the colony of the strain was round, the surface was moist and smooth, the edge was clean and colorless and semitransparent (see FIG. 1).
(2) Molecular biological Properties
The 16s rDNA gene sequence of this strain was determined as follows (SEQ-1):
TATAAAGCTACCTACTTCTTTTGCAGCCCACTCCCATGGTGTGACGGGCGGTGTGTA CAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTTTGGGATTCGCTC ACTTTCGCAAGTTGGCTGCCCTCTGTATGCGCCATTGTAGCACGTGTGTAGCCCTACTCGTAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTC CCTGGAGTTCCCGACATTACTCGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG ACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAGAGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCTCTGGATGTCAAGAGTAGGTAAGGT TCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATT CATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTCCGAAAGCCACGGCTCAAGGCGACGACCTCCAAGTAGACATCGTTTACGGCGTGGACTAC CAGGGTATGTAATCCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGTCAGTATCTGTCCAGGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATCTCTACGCATTTCACCGCTACACCT GAAATTCTACCCCCCCTCTACAGTACTCTAGTCTGCCAGTTTCAAATGCAATTCCGAGGTTGAGCCCCGGGCTTTCACATCTGACTTAACAAACCACCTGCATGCGCTTTACGCCCAGTA ATTCCGATTAACGCTCGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGTCGCTAACGTCAAATAATGCAGCTATTAACTACACTACCTTCCTCACGACT GAAAGTGCTTTACAACCCGAAGGCCTTCTTCACACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAG TTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTTACCTCACCAACTAGCTAATCCCACCTAGGCATATCCTGACGCGAGAGGCCCGAAGGTCC CCCTCTTTGGCCCGTAGGCATCATGCGGTATTAGCCATCGTTTCCAATGGTTATCCCCCACATCAGGGCAATTTCCTAGGCATTACTCACCCGTCCGCCGCTCGACGCCGTTATCGTCCCC CGAAGGTTCTGACAACTCGTTCCGCTCGACTGCATGG
the strain HT-10 is identified as Vibrio azureus (Vibrio azureus) by morphology and molecular biology, and the strain is preserved in China general microbiological culture Collection center (China Committee for culture Collection), and the preservation date is that: and 2021, 9 and 18 days, wherein the preservation number is CGMCC NO.23443.
Example 2: preparation of Vibrio coelicolor HT-10 shake flask microbial agent
(1) A small amount of purified strain is dipped in an inoculating loop and placed in a 50mL triangular flask containing 10mL of seed liquid culture medium, and the seed liquid is obtained after constant-temperature culture for 24 hours at the temperature of 20 ℃ at 120 r/min;
seed liquid medium, i.e. NB medium containing 3.5% nacl: 10g of peptone, 3g of beef powder, 35g of NaCl, 1000mL of water and pH value of 7.3+/-0.1;
(2) Inoculating the seed solution prepared in the step (1) into a shake flask filled with a fermentation medium, wherein the inoculation ratio is 1:50, and the fermentation conditions are the same as those in the step (1), so as to obtain a fermentation liquor (shake flask microbial inoculum) of the Vibrio coerulescens HT-10 strain, and the viable count of the fermentation liquor is 1-5 multiplied by 10 10 cfu/g. The fermentation of each strain is carried out in shake flasks to obtain shake flask microbial inoculum of each strain.
The fermentation medium (1000 ml) is: 20g of glucose, 20g of starch, 15g of yeast extract powder, 4g of fish peptone, 0.5g of magnesium sulfate, 0.5g of monopotassium phosphate, 6g of calcium carbonate and the balance of seawater, wherein the pH value is 7.5+/-0.1.
Example 3: vibrio coeruleus HT-10 degradation function research
(1) Functional verification of Vibrio coerulescens HT-10 degraded enteromorpha
Vibrio coelicolor HT-10 shake flask microbial agent is prepared according to example 2; weighing 5g of fresh enteromorpha, and placing in a test tube, wherein the compactness is kept consistent; 1mL of Vibrio azurii HT-10 microbial inoculum is added into a test tube, and 1mL of fermentation medium is added into a control group; each treatment was repeated 3 times; the plugs are plugged, and an anaerobic environment is provided; and (3) observing at 20 ℃ at 3d, 5d, 7d and 9d respectively, and recording the appearance state of the enteromorpha.
As shown in fig. 2, compared with the control group (CK), after 3d of applying the vibrio coelicolor HT-10 microbial inoculum, the color of the enteromorpha is changed from green to light, and the enteromorpha is seriously hydrated; after 5d of treatment, the enteromorpha starts blackening; after 7d of treatment, the enteromorpha is basically completely blackened; after 9d of treatment, all the enteromorpha is seriously blackened. In conclusion, the research results initially prove that the Vibrio coerulescens HT-10 obtained by screening has excellent enteromorpha degrading capability, and the results are consistent with the screening results; meanwhile, the degradation function of the Vibrio paradise HT-10 microbial inoculum is stable.
(2) Vibrio coeruleus HT-10 degradation capability detection
Vibrio coelicolor HT-10 shake flask microbial agent is prepared according to example 2; weighing 1Kg of fresh enteromorpha and placing the fresh enteromorpha in a sealed bag; 200mL of Vibrio paradise HT-10 microbial inoculum is added into a sealed bag, and 200mL of fermentation medium is added into a control group; each treatment was repeated 3 times; sealing to provide an anaerobic environment; and (3) observing at 20 ℃ for 9d, and recording the appearance state of the enteromorpha.
The dry matter content, polysaccharide content, crude protein content and fat content of Enteromorpha prolifera before and after treatment were respectively detected by a drying weighing method, a phenol-sulfuric acid method, a Kelvin semi-trace nitrogen determination method and a Soxhlet extraction method, and the changes of the four index contents were counted, as shown in Table 2.
As shown in the figure 3, after 9d of treatment by the Vibrio coerulescens HT-10 microbial inoculum, the enteromorpha is seriously blackened and hydrated; the color of the enteromorpha part of the control group begins to lighten; this initially shows that Vibrio coelicolor HT-10 has strong enteromorpha degrading capability. Through the content detection of enteromorpha dry matter, polysaccharide, crude protein and fat before and after treatment, the dry matter is reduced by 30.1 percent, the crude polysaccharide is reduced by 13.5 percent, the crude protein is reduced by 9.1 percent and the crude fat is reduced by 2.7 percent after being treated by the Vibrio coerulescens HT-10 microbial agent (refer to table 2).
TABLE 2 Vibrio coerulescens HT-10 related component content variation after treatment
Meanwhile, the degradation products obtained after the enteromorpha is fully fermented and degraded are sent to the scientific and technological company of Stansted laboratory, suzhou for detection, and the glucose content, the total amino acid and polypeptide content and the fatty acid content of the degradation products are detected. The results show that after being degraded by Vibrio coerulescens HT-10, the main components of the enteromorpha degradation are micromolecular glucose, amino acid, polypeptide and fatty acid, and the contents of the micromolecular glucose, the amino acid, the polypeptide and the fatty acid are 46.3%, 12.7% and 0.3% respectively (refer to Table 3).
TABLE 3 essential components of Enteromorpha degradation after Vibrio coerulescens HT-10 treatment
The results show that the Vibrio coerulescens HT-10 can degrade the enteromorpha with high efficiency, degrade macromolecular crude polysaccharide, crude protein and crude fat into micromolecular glucose, amino acid, polypeptide and fatty acid degradation enteromorpha products, the degradation components, ash and salt in the enteromorpha can be just made into fine feed, and the degradation products can be added with other components according to the feed formula requirement to be simply mixed and directly granulated to obtain the feed. From the results, it can also be demonstrated that Vibrio coelicolor HT-10 produced degrading enzymes are rich; meanwhile, the microbial inoculum is low in cost and convenient to obtain, and can be used for production as a functional strain of a subsequent enteromorpha degradation microbial inoculum.
SEQUENCE LISTING
<110> Shangqi Biotech Co., ltd in Qingdao
<120> Vibrio azurii and application thereof in degrading enteromorpha
<130> 0
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1408
<212> DNA
<213> 16s rDNA Gene sequence of Vibrio paradise (Vibrio azureus) HT-10
<400> 1
tataaagcta cctacttctt ttgcagccca ctcccatggt gtgacgggcg gtgtgtacaa 60
ggcccgggaa cgtattcacc gtggcattct gatccacgat tactagcgat tccgacttca 120
tggagtcgag ttgcagactc caatccggac tacgacgcac tttttgggat tcgctcactt 180
tcgcaagttg gctgccctct gtatgcgcca ttgtagcacg tgtgtagccc tactcgtaag 240
ggccatgatg acttgacgtc gtccccacct tcctccggtt tatcaccggc agtctccctg 300
gagttcccga cattactcgc tggcaaacaa ggataagggt tgcgctcgtt gcgggactta 360
acccaacatt tcacaacacg agctgacgac agccatgcag cacctgtctc agagttcccg 420
aaggcaccaa tccatctctg gaaagttctc tggatgtcaa gagtaggtaa ggttcttcgc 480
gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca attcatttga 540
gttttaatct tgcgaccgta ctccccaggc ggtctactta acgcgttagc tccgaaagcc 600
acggctcaag gcgacgacct ccaagtagac atcgtttacg gcgtggacta ccagggtatg 660
taatcctgtt tgctccccac gctttcgcat ctgagtgtca gtatctgtcc aggggggccg 720
ccttcgccac cggtattcct tcagatctct acgcatttca ccgctacacc tgaaattcta 780
ccccccctct acagtactct agtctgccag tttcaaatgc aattccgagg ttgagccccg 840
ggctttcaca tctgacttaa caaaccacct gcatgcgctt tacgcccagt aattccgatt 900
aacgctcgca ccctccgtat taccgcggct gctggcacgg agttagccgg tgcttcttct 960
gtcgctaacg tcaaataatg cagctattaa ctacactacc ttcctcacga ctgaaagtgc 1020
tttacaaccc gaaggccttc ttcacacacg cggcatggct gcatcaggct tgcgcccatt 1080
gtgcaatatt ccccactgct gcctcccgta ggagtctgga ccgtgtctca gttccagtgt 1140
ggctgatcat cctctcagac cagctaggga tcgtcgcctt ggtgagccct tacctcacca 1200
actagctaat cccacctagg catatcctga cgcgagaggc ccgaaggtcc ccctctttgg 1260
cccgtaggca tcatgcggta ttagccatcg tttccaatgg ttatccccca catcagggca 1320
atttcctagg cattactcac ccgtccgccg ctcgacgccg ttatcgtccc ccgaaggttc 1380
tgacaactcg ttccgctcga ctgcatgg 1408
Claims (9)
1. Vibrio azurii (Fr.) kurzVibrio azureus) HT-10, wherein the preservation number of the strain is CGMCC NO.23443.
2. The use of Vibrio coelicolor HT-10 according to claim 1 for degrading enteromorpha.
3. The use according to claim 2, wherein the degraded enteromorpha is a dry matter, crude polysaccharide, crude protein and crude fat of degraded enteromorpha.
4. The fermentation process of Vibrio coerulescens HT-10 according to claim 1, wherein,
1) Culturing 24+ -12 h in NB medium containing 3.5% NaCl at 20+ -2deg.C to obtain seed solution of Vibrio coerulescens HT-10;
2) Inoculating the seed solution of Vibrio coerulescens HT-10 into a fermentation medium, and fermenting and culturing at 20+/-2 ℃ for 24+/-12 h to obtain the fermentation solution of Vibrio coerulescens HT-10.
5. The fermentation process of claim 4, wherein the NB medium containing 3.5% NaCl is: peptone 10g, beef powder 3g, naCl 35g, 1000mL of water, pH 7.3+ -0.1, and the above proportions.
6. The fermentation process of claim 4, wherein the fermentation medium is 1000 ml: glucose 20g, starch 20g, yeast extract 15g, fish peptone 4g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 0.5g, calcium carbonate 6g, and seawater as the rest, wherein the pH value is 7.5+ -0.1, and the above proportions are prepared.
7. The Vibrio coerulescens HT-10 fermentation broth prepared by the fermentation process of any one of claims 4-6.
8. The use of the Vibrio coerulescens HT-10 fermentation broth according to claim 7 for degrading Enteromorpha prolifera.
9. A method for degrading enteromorpha by utilizing Vibrio coerulescens HT-10 is characterized in that Vibrio coerulescens HT-10 fermentation liquor according to claim 7 is directly put into fresh enteromorpha, and anaerobic fermentation is carried out for 8-15 days at 15-25 ℃ under a sealed condition.
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