CN114410542A

CN114410542A - Vibrio sky blue and application thereof in enteromorpha degradation

Info

Publication number: CN114410542A
Application number: CN202210138022.9A
Authority: CN
Inventors: 张兴忠; 张淑静; 徐美萍; 王学; 袁红晓; 苏本涛; 赵林
Original assignee: Qingdao Zhongshangqi Biotechnology Co ltd
Current assignee: Qingdao Zhongshangqi Biotechnology Co ltd
Priority date: 2022-02-15
Filing date: 2022-02-15
Publication date: 2022-04-29
Anticipated expiration: 2042-02-15
Also published as: CN114410542B

Abstract

The invention discloses a Vibrio sky blue and application thereof in degrading enteromorpha.A water sample containing rotten enteromorpha is separated by adopting a high-salt culture medium, and the Vibrio sky blue (Vibrio azureus) HT-10 with better enteromorpha degrading effect is screened by verifying the degrading effect, and the strain is preserved in the China general microbiological culture Collection center at 9 and 18 months 2021, and the preservation number is CGMCC NO. 23443. The bacterial colony of the strain on the NA culture medium containing 3.5% NaCl is circular, the surface is wet and smooth, the edge is neat, and the strain is colorless and semitransparent. The strain can directly act on enteromorpha and efficiently degrade the enteromorpha, is easy for fermentation production, and can replace the conventional complex treatment mode at present; the method for degrading enteromorpha by adopting the strain is simple and easy to implement, and is suitable for large-area popularization.

Description

Vibrio sky blue and application thereof in enteromorpha degradation

Technical Field

The invention relates to a vibrio sky-blue and application thereof in degrading enteromorpha, belonging to the technical field of microorganisms.

Background

Enteromorpha prolifera (Enteromorpha prolifera) belongs to algae plants of Ulvaceae and Enteromorpha, is a large-scale filamentous economic algae which is extremely rich in marine wild plants in China, and has important development value. In recent years, due to a plurality of factors such as seawater eutrophication and ocean water environment warming, the enteromorpha prolifera grows and breeds explosively, and a disastrous green tide is formed; a large amount of enteromorpha is accumulated, the development of marine ecological environment and offshore tourism industry is seriously influenced, and great loss is brought to the mariculture industry. At present, the most common method for treating enteromorpha is centralized stacking and simple burying, and the method can generate a large amount of sewage and stink in the anaerobic fermentation process and seriously affect the surrounding environment. Therefore, how to rapidly process the enteromorpha becomes a problem which needs to be solved urgently at present, and the problem also becomes a technical problem.

Under the large background, people gradually turn to the aim of screening the microbial degradation bacteria to recycle enteromorpha. More than 70% of enteromorpha is crude polysaccharide, so people mainly focus on how to utilize the crude polysaccharide of enteromorpha on a single substance, neglecting that the crude polysaccharide additionally contains about 13% of crude protein, about 15% of ash, 1% of crude fat and the like, failing to comprehensively utilize the enteromorpha, and causing resource waste; meanwhile, the research on the enteromorpha crude polysaccharide mainly focuses on degrading the crude polysaccharide into reducing sugar by utilizing microbial polysaccharide degrading enzyme, and the method is used for biological energy research. Therefore, the high-efficiency degrading bacteria capable of directly acting on the enteromorpha are screened, and the enteromorpha degradation product is comprehensively utilized to become the optimal utilization mode of the enteromorpha.

But few researches on the enteromorpha high-efficiency degrading bacteria are carried out at present. The patent CN 113293114A discloses alteromonas HT1 and a culture method and application thereof, the microorganism preservation number of the alteromonas is CCTCC NO.M 2021217, the strain can grow in a culture medium taking enteromorpha as a unique carbon source, and a product obtained by degrading enteromorpha polysaccharide is enteromorpha oligosaccharide which mainly exists in the forms of disaccharide, trisaccharide, tetrasaccharide and pentasaccharide. Patent CN 103740597A discloses a penicillium oxalicum strain and a screening method and application thereof, the microorganism preservation number of the penicillium oxalicum strain is CGMCC NO.8270, the strain has good degradation effect on cellulose substances in enteromorpha, and the yield of reducing sugar is measured to be 8.22%. Patent CN108753643A discloses a vibrio H11 for efficiently hydrolyzing enteromorpha polysaccharide, wherein the preservation number of the microbe is CCTCC NO: m2018172, the reducing sugar obtained by hydrolysis of which can be utilized by energy strains to produce bioenergy substances. As can be seen, at present, the variety of the enteromorpha prolifera high-efficiency degrading bacteria is very few, the enteromorpha prolifera high-efficiency degrading bacteria are only integrated in degrading enteromorpha prolifera polysaccharide, and the reports of the enteromorpha prolifera degradation by vibrio coelicolor are not seen.

Disclosure of Invention

Aiming at the problems, the invention provides a Vibrio sky blue (Vibrio azureus) HT-10 strain capable of efficiently degrading enteromorpha and an application thereof, wherein the strain is capable of efficiently degrading enteromorpha by combining hydrolysis and anaerobic fermentation of the Vibrio, and has a wide application prospect in development and utilization of enteromorpha resources.

The above purpose of the invention is realized by the following technical scheme: the invention adopts a high-salt culture medium to separate a water sample containing rotten enteromorpha and screens and verifies and screens the Vibrio azureum HT-10 with better enteromorpha degradation effect through degradation effect, the strain is preserved in China general microbiological culture Collection center (CGMCC) at 9.18.2021, the address of a preservation unit, No. 3 of West Lu No. 1 of the sunward area of Beijing, and the preservation number is CGMCC NO. 23443. The bacterial colony of the strain on the NA culture medium containing 3.5% NaCl is circular, the surface is wet and smooth, the edge is neat, and the strain is colorless and semitransparent.

The invention also provides a fermentation method of the Vibrio azurea HT-10, which is characterized in that,

1) culturing in NB culture medium containing 3.5% NaCl at 20 + -2 deg.C for 24 + -12 h to obtain seed solution of Vibrio sky blue HT-10;

2) inoculating the seed solution of Vibrio sky blue HT-10 into a fermentation culture medium, and performing fermentation culture at 20 + -2 deg.C for 24 + -12 h to obtain fermentation liquid of Vibrio sky blue HT-10.

NB medium with 3.5% NaCl was: 10g of peptone, 3g of beef powder, 35g of NaCl and 1000mL of water, and the pH value is 7.3 +/-0.1.

The fermentation medium (1000ml) was: 20g of glucose, 20g of starch, 15g of yeast extract powder, 4g of fish peptone, 0.5g of magnesium sulfate, 0.5g of monopotassium phosphate and 6g of calcium carbonate, wherein the balance is seawater and the pH value is 7.5 +/-0.1.

Another purpose of the invention is to provide application of Vibrio azurea HT-10 in degrading enteromorpha.

The degraded enteromorpha is dry matter, crude polysaccharide, crude protein and crude fat of the degraded enteromorpha.

The invention also provides a method for degrading enteromorpha by using Vibrio azurea (Vibrio azureus) HT-10, which is characterized in that the Vibrio azurea (Vibrio azureus) HT-10 fermentation liquor is directly put into fresh enteromorpha, anaerobic fermentation is carried out at 15-25 ℃ under a sealed condition, degradation is finished within 8-15 days, macromolecular crude polysaccharide, crude protein and crude fat are degraded into micromolecular glucose, amino acid, polypeptide and fatty acid, and the degradation product can be directly used for feed production.

The invention has the technical effects that:

1. according to the invention, a water sample containing rotten enteromorpha is separated by adopting a high-salt culture medium, and through degradation effect verification and screening, the Vibrio azureus HT-10 with a good enteromorpha degradation effect is obtained, after enteromorpha is treated by using the bacterial agent of the bacterial strain, the dry matter of the enteromorpha is reduced by 30.1%, the polysaccharide substance is reduced by 13.5%, the crude protein is reduced by 9.1%, and the fat is reduced by 2.7%; the strain can directly act on the enteromorpha and efficiently degrade the enteromorpha.

2. The vibrio sky blue HT-10 is easy to ferment and produce, and the degraded enteromorpha product can be directly used for feed production; the mode of directly acting on the enteromorpha can replace the conventional complex treatment mode at present; the method is simple and easy to implement, and is suitable for large-area popularization; the strain has wide application prospect in the development and utilization of enteromorpha resources.

Drawings

FIG. 1 is a colony morphology of Vibrio sky blue HT-10;

FIG. 2 shows the change of the appearance state of Enteromorpha (in a test tube) after the Vibrio coelicolor HT-10 microbial inoculum is treated for different days;

FIG. 3 is the change of appearance state (in a sealed bag) of Enteromorpha prolifera treated by Vibrio coelicolor HT-10 microbial inoculum.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the contents in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1: isolation and characterization of strains

1. Isolation of Strain HT-10

A water sample containing rotten enteromorpha is collected from a sea water bathing place in yellow island region of Qingdao city, Shandong province, and is separated on an NA culture medium (high-salt culture medium) containing 3.5% NaCl by using a dilution coating method. The specific method comprises the following steps:

(1) and (3) sterilizing the articles: sterilizing the culture dish, the gun head, the sterile water and other experimental articles under 0.1MPa and 121 ℃ for 30min for later use;

(2) obtaining a bacterial sample: mixing the water sample and the smashed and rotten enteromorpha according to a ratio of 9:1, placing the mixture in an aseptic triangular flask containing glass beads, oscillating the mixture for 30min under the condition of 120r/min, and filtering the mixture by four layers of aseptic gauze to obtain a bacterial sample;

(3) bacterial sample dilution: 6 test tubes containing 9mL of sterile water are sequentially numbered 10^-1～10^-6(ii) a Sucking 1mL of the strain sample from the triangular flask, and placing the strain sample into a flask with the standard 10^-1In a test tube, i.e. 10^-1Diluting; and again indicated with 10^-1Taking 1mL of the diluted bacterial solution, and putting the diluted bacterial solution into a container marked with 10^-2In a test tube, i.e. 10^-2Diluting; sequentially prepared by the same method to obtain 10^-3、10^-4、10^-5、10^-6A diluted bacterial sample;

(4) preparation of the Medium

Isolation medium (high salt medium), i.e. NA medium containing 3.5% NaCl: 10g of peptone, 3g of beef powder, 35g of NaCl, 15g of agar and 1000mL of water, wherein the pH value is 7.3 +/-0.1;

purifying the culture medium and separating the culture medium;

(5) coating: diluting the bacterial sample 10^-3、10^-4、10^-5、10^-6Respectively coating on a separation culture medium flat plate, each gradient 3 dish, and marking on the bottom of the dish;

(6) culturing: after coating, carrying out inverted culture at a constant temperature of 20 ℃ for about 3 days, and observing;

(8) strain purification: picking single colony with inoculating hook, streaking on plate containing purified culture medium, and reverse culturing at 20 deg.C for 3d to pick single colony.

The results show that 30 strains of bacteria were isolated in total on a high salt medium and numbered HT-01-HT-30, respectively.

2. Strain HT-10 screening

Carrying out shake flask culture on the separated strains for 24h, and then preparing shake flask inocula of different strains (see example 2) for later use; weighing 5g of fresh enteromorpha and placing the fresh enteromorpha in a test tube to keep the consistency of compactness; adding 1mL of shake flask microbial inoculum of different strains into each test tube, plugging a plug and providing an anaerobic environment; and (5) observing at 20 ℃ after 5 d.

The result shows that the enteromorpha inoculated with the HT-10 microbial inoculum becomes black after being treated for 5 days; the enteromorpha inoculated with the HT-07 microbial inoculum turns brown and is seriously hydrated; the color of the enteromorpha inoculated with HT-05, HT-08, HT-15, HT-18 and HT-22 bactericides is changed from green to light. The results preliminarily show that HT-05, HT-07, HT-08, HT-10, HT-15, HT-18 and HT-22 have the function of degrading enteromorpha, and the effects of HT-10 and HT-07 are superior to those of other strains.

TABLE 1 summary of degradation of Enteromorpha by different strains

The criteria for judging the activity level are: the enteromorpha is black-starting grade of +++, the enteromorpha is brown and hydrated and is of +++, the enteromorpha is green-lightening grade of ++, and the enteromorpha is non-discoloring grade of- ".

3. Morphological and molecular biological identification of strain HT-10

(1) Morphological characteristics: the strain HT-10 is inoculated on an NA culture medium containing 3.5% NaCl and cultured at the constant temperature of 20 ℃ for 24h to observe the colony characteristics. As a result, the colony of the strain was round, the surface was wet and smooth, the edge was neat, and the strain was colorless and translucent (see FIG. 1).

(2) Molecular biological Properties

The sequence determination result of the 16s rDNA gene of the strain is as follows (SEQ-1):

TATAAAGCTACCTACTTCTTTTGCAGCCCACTCCCATGGTGTGACGGGCGGTGTGTA CAAGGCCCGGGAACGTATTCACCGTGGCATTCTGATCCACGATTACTAGCGATTCCGACT TCATGGAGTCGAGTTGCAGACTCCAATCCGGACTACGACGCACTTTTTGGGATTCGCTC ACTTTCGCAAGTTGGCTGCCCTCTGTATGCGCCATTGTAGCACGTGTGTAGCCCTACTCG TAAGGGCCATGATGACTTGACGTCGTCCCCACCTTCCTCCGGTTTATCACCGGCAGTCTC CCTGGAGTTCCCGACATTACTCGCTGGCAAACAAGGATAAGGGTTGCGCTCGTTGCGGG ACTTAACCCAACATTTCACAACACGAGCTGACGACAGCCATGCAGCACCTGTCTCAGAG TTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCTCTGGATGTCAAGAGTAGGTAAGGT TCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATT CATTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGTCTACTTAACGCGTTAGCTCC GAAAGCCACGGCTCAAGGCGACGACCTCCAAGTAGACATCGTTTACGGCGTGGACTAC CAGGGTATGTAATCCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGTCAGTATCTGTCCA GGGGGGCCGCCTTCGCCACCGGTATTCCTTCAGATCTCTACGCATTTCACCGCTACACCT GAAATTCTACCCCCCCTCTACAGTACTCTAGTCTGCCAGTTTCAAATGCAATTCCGAGGT TGAGCCCCGGGCTTTCACATCTGACTTAACAAACCACCTGCATGCGCTTTACGCCCAGTA ATTCCGATTAACGCTCGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGT GCTTCTTCTGTCGCTAACGTCAAATAATGCAGCTATTAACTACACTACCTTCCTCACGACT GAAAGTGCTTTACAACCCGAAGGCCTTCTTCACACACGCGGCATGGCTGCATCAGGCTT GCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAG TTCCAGTGTGGCTGATCATCCTCTCAGACCAGCTAGGGATCGTCGCCTTGGTGAGCCCTT ACCTCACCAACTAGCTAATCCCACCTAGGCATATCCTGACGCGAGAGGCCCGAAGGTCC CCCTCTTTGGCCCGTAGGCATCATGCGGTATTAGCCATCGTTTCCAATGGTTATCCCCCAC ATCAGGGCAATTTCCTAGGCATTACTCACCCGTCCGCCGCTCGACGCCGTTATCGTCCCC CGAAGGTTCTGACAACTCGTTCCGCTCGACTGCATGG

the strain HT-10 is identified as Vibrio azureum by morphology and molecular biology, and the strain is preserved in the China general microbiological culture Collection center, and the preservation date is as follows: the preservation number is CGMCC NO.23443 at 18 months 9 in 2021.

Example 2: preparation of Vibrio sky blue HT-10 shake flask microbial inoculum

(1) Dipping a small amount of purified strains by using an inoculating loop into a 50mL triangular flask containing 10mL of seed liquid culture medium, and culturing at constant temperature of 120r/min and 20 ℃ for 24h to obtain seed liquid;

seed liquid culture medium, i.e. 3.5% NaCl NB-containing medium: 10g of peptone, 3g of beef powder, 35g of NaCl and 1000mL of water, wherein the pH value is 7.3 +/-0.1;

(2) inoculating the seed solution prepared in the step (1) into a shake flask filled with a fermentation culture medium, wherein the inoculation ratio is 1:50, the fermentation conditions are the same as those in the step (1), and a fermentation liquid (shake flask microbial inoculum) of the HT-10 strain of the vibrio sky blue is obtained, and the viable count of the fermentation liquid is 1-5 multiplied by 10¹⁰cfu/g. The shake flask microbial inoculum of each strain can be obtained by fermenting each strain in a shake flask.

Example 3: research on degradation function of Vibrio sky blue HT-10

(1) Functional verification of enteromorpha degradation of vibrio coeruleus by virtue of Vibrio sky blue HT-10

See example 2 for shake flask preparation of Vibrio sky blue HT-10; weighing 5g of fresh enteromorpha and placing the fresh enteromorpha in a test tube to keep the consistency of compactness; adding 1mL of vibrio sky blue HT-10 microbial inoculum into a test tube, and adding 1mL of fermentation medium into a control group; each treatment was repeated 3 times; the plugs are plugged uniformly to provide an anaerobic environment; and (5) observing at 3d, 5d, 7d and 9d respectively under the condition of 20 ℃, and recording the appearance state of the enteromorpha.

As shown in FIG. 2, compared with the control group (CK), after the application of the Vibrio sky blue HT-10 microbial inoculum for 3d, the color of the Enteromorpha prolifera is changed from green to light, and the Enteromorpha prolifera is severely hydrated; after the treatment for 5d, the enteromorpha starts to blacken; after 7d of treatment, basically all enteromorpha is blackened; after 9 days of treatment, the enteromorpha prolifera is completely blackened seriously. In conclusion, the research results preliminarily prove that the screened vibrio sky blue HT-10 has excellent enteromorpha degrading capability, and the results are consistent with the screening results; meanwhile, the degradation function of the Vibrio sky blue HT-10 microbial inoculum is stable.

(2) Detection of the degrading ability of Vibrio sky blue HT-10

See example 2 for shake flask preparation of Vibrio sky blue HT-10; weighing 1Kg of fresh enteromorpha and placing in a sealed bag; adding 200mL of vibrio sky blue HT-10 microbial inoculum into the sealed bag, and adding 200mL of fermentation medium into the control group; each treatment was repeated 3 times; sealing to provide an anaerobic environment; and (5) observing at 9d under the condition of 20 ℃, and recording the appearance state of the enteromorpha prolifera.

The dry matter content, polysaccharide content, crude protein content and fat content of enteromorpha before and after treatment were respectively detected by a drying and weighing method, a phenol-sulfuric acid method, a Kjeldahl semi-micro nitrogen determination method and a Soxhlet extraction method, and the content change of four indexes was counted, as shown in Table 2.

The result is shown in figure 3, after the enteromorpha is treated by the Vibrio sky blue HT-10 microbial inoculum for 9 days, the enteromorpha is severely blackened and hydrated; the color of part of the enteromorpha of the control group begins to become lighter; this preliminarily shows that Vibrio sky blue HT-10 has strong ability to degrade Enteromorpha. Through content detection of dry matter, polysaccharide, crude protein and fat of enteromorpha before and after treatment, after treatment by using the vibrio sky blue HT-10 microbial inoculum, the dry matter is reduced by 30.1%, the crude polysaccharide is reduced by 13.5%, the crude protein is reduced by 9.1%, and the crude fat is reduced by 2.7% (see table 2).

TABLE 2 change in the content of relevant components of Enteromorpha after HT-10 treatment of Vibrio coelicolor

Meanwhile, the degradation product obtained after full fermentation and degradation of the enteromorpha is sent to Suzhou Stentde laboratory science and technology limited company for detection, and the glucose content, the total amount of amino acid and polypeptide and the fatty acid content of the degradation product are detected. The results show that after the degradation of the vibrio sky blue HT-10, the main components of the enteromorpha degradation product are small molecular glucose, amino acid, polypeptide and fatty acid, and the contents are 46.3%, 12.7% and 0.3% respectively (refer to Table 3).

TABLE 3 major component of enteromorpha degradation product after HT-10 treatment of Vibrio coelicolor

The results show that the vibrio sky blue HT-10 can efficiently degrade enteromorpha, macromolecular crude polysaccharide, crude protein and crude fat are degraded into products of degrading the enteromorpha by micromolecular glucose, amino acid, polypeptide and fatty acid, the degraded components and ash content and salt content in the enteromorpha can be prepared into fine feed, the degraded products can be added with other components according to the feed formula requirements, and the feed can be obtained by direct granulation. From the results, it can also be shown that the degrading enzymes produced by Vibrio sky blue HT-10 are abundant; meanwhile, the microbial inoculum is low in cost and convenient to obtain, and can be used as a functional strain of a subsequent enteromorpha degrading microbial inoculum for production.

SEQUENCE LISTING

<110> Hipposhu Biotech Co., Ltd in Qingdao

<120> Vibrio sky blue and application thereof in enteromorpha degradation

<130> 0

<160> 1

<170> PatentIn version 3.3

<210> 1

<211> 1408

<212> DNA

<213> 16s rDNA Gene sequence of Vibrio azureum HT-10

<400> 1

tataaagcta cctacttctt ttgcagccca ctcccatggt gtgacgggcg gtgtgtacaa 60

ggcccgggaa cgtattcacc gtggcattct gatccacgat tactagcgat tccgacttca 120

tggagtcgag ttgcagactc caatccggac tacgacgcac tttttgggat tcgctcactt 180

tcgcaagttg gctgccctct gtatgcgcca ttgtagcacg tgtgtagccc tactcgtaag 240

ggccatgatg acttgacgtc gtccccacct tcctccggtt tatcaccggc agtctccctg 300

gagttcccga cattactcgc tggcaaacaa ggataagggt tgcgctcgtt gcgggactta 360

acccaacatt tcacaacacg agctgacgac agccatgcag cacctgtctc agagttcccg 420

aaggcaccaa tccatctctg gaaagttctc tggatgtcaa gagtaggtaa ggttcttcgc 480

gttgcatcga attaaaccac atgctccacc gcttgtgcgg gcccccgtca attcatttga 540

gttttaatct tgcgaccgta ctccccaggc ggtctactta acgcgttagc tccgaaagcc 600

acggctcaag gcgacgacct ccaagtagac atcgtttacg gcgtggacta ccagggtatg 660

taatcctgtt tgctccccac gctttcgcat ctgagtgtca gtatctgtcc aggggggccg 720

ccttcgccac cggtattcct tcagatctct acgcatttca ccgctacacc tgaaattcta 780

ccccccctct acagtactct agtctgccag tttcaaatgc aattccgagg ttgagccccg 840

ggctttcaca tctgacttaa caaaccacct gcatgcgctt tacgcccagt aattccgatt 900

aacgctcgca ccctccgtat taccgcggct gctggcacgg agttagccgg tgcttcttct 960

gtcgctaacg tcaaataatg cagctattaa ctacactacc ttcctcacga ctgaaagtgc 1020

tttacaaccc gaaggccttc ttcacacacg cggcatggct gcatcaggct tgcgcccatt 1080

gtgcaatatt ccccactgct gcctcccgta ggagtctgga ccgtgtctca gttccagtgt 1140

ggctgatcat cctctcagac cagctaggga tcgtcgcctt ggtgagccct tacctcacca 1200

actagctaat cccacctagg catatcctga cgcgagaggc ccgaaggtcc ccctctttgg 1260

cccgtaggca tcatgcggta ttagccatcg tttccaatgg ttatccccca catcagggca 1320

atttcctagg cattactcac ccgtccgccg ctcgacgccg ttatcgtccc ccgaaggttc 1380

tgacaactcg ttccgctcga ctgcatgg 1408

Claims

1. A Vibrio sky blue (Vibrio azureus) HT-10 strain has a preservation number of CGMCC NO. 23443.

2. The Vibrio sky-blue HT-10 according to claim 1, wherein the colony of said strain is round on NA medium containing 3.5% NaCl, has a wet and smooth surface, has a neat edge, and is colorless and translucent.

3. Application of Vibrio sky blue HT-10 in degrading Enteromorpha prolifera is provided.

4. The use of claim 3, wherein the degraded Enteromorpha prolifera is degraded in dry matter, crude polysaccharides, crude proteins and crude fats of Enteromorpha prolifera.

5. The fermentation method of Vibrio sky blue HT-10 according to claim 1,

6. The fermentation process according to claim 5, wherein the NB medium containing 3.5% NaCl is: 10g of peptone, 3g of beef powder, 35g of NaCl and 1000mL of water, and the pH value is 7.3 +/-0.1, and the components are prepared according to the proportion.

7. The fermentation process according to claim 5, wherein the fermentation medium comprises, in 1000 ml: 20g of glucose, 20g of starch, 15g of yeast extract powder, 4g of fish peptone, 0.5g of magnesium sulfate, 0.5g of monopotassium phosphate and 6g of calcium carbonate, wherein the balance is seawater, and the pH value is 7.5 +/-0.1.

8. The Vibrio sky-blue HT-10 fermentation broth produced by the fermentation process of any one of claims 5 to 7.

9. The use of the Vibrio sky blue HT-10 fermentation broth of claim 8 for degrading Enteromorpha.

10. A method for degrading enteromorpha by using Vibrio sky blue HT-10 is characterized in that the Vibrio sky blue HT-10 fermentation liquid of claim 8 is directly put into fresh enteromorpha, and is subjected to anaerobic fermentation for 8-15 days at 15-25 ℃ under sealed conditions.