CN114306157A - Composite plant skin barrier protective agent and preparation method and application thereof - Google Patents
Composite plant skin barrier protective agent and preparation method and application thereof Download PDFInfo
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- CN114306157A CN114306157A CN202111635446.8A CN202111635446A CN114306157A CN 114306157 A CN114306157 A CN 114306157A CN 202111635446 A CN202111635446 A CN 202111635446A CN 114306157 A CN114306157 A CN 114306157A
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Abstract
The invention belongs to the technical field of cosmetics, and discloses a composite plant skin barrier protective agent, and a preparation method and application thereof. Specifically disclosed is a composition comprising the following components: radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract and radix Pulsatillae extract. The composition can effectively supplement water and nutrients lost by skin, maintain the pH value required by cell survival, especially keep stable differentiation of stratum corneum cells, and provide sufficient nutrients to repair damaged skin.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a composite plant skin barrier protective agent, and a preparation method and application thereof.
Background
The skin is the organ most in direct contact with the outside world and is the first physiological barrier of the human immune system. Firstly, external adverse factors such as biological, physical and chemical factors can be prevented from entering a human body to a certain extent to damage the human body; and secondly, the loss of substances such as water, lipid and the like in the epidermis and the dermis of the human body can be avoided, so that the skin barrier function is maintained to be stable, and particularly, the stratum corneum in the epidermis plays an important role in the skin barrier.
Once the skin is damaged, the protective function of the barrier cannot be effectively exerted, and physical damage, chemical damage and biological damage are common. Common features are that the stratum corneum in the epidermis is damaged to varying degrees, typically showing loss of water and nutrients, changes in pH, and a disruption in the structural integrity of the skin. The skin of people with sensitive muscles becomes thin, so that anaphylactic reaction is easy to occur, and the skin is red and swollen.
The skin care products for repairing skin barriers commonly used at present have some disadvantages: one is to focus on efficacy alone, but neglect the health of the skin barrier. Secondly, only care is paid to a single barrier, other barriers of the skin are ignored, the surface of the skin looks good, and the problem of deep skin is difficult to solve. The development of a skin care product capable of repairing the skin barrier is urgently needed, the effect of effectively and rapidly repairing the damaged skin barrier is achieved, and the skin care product is particularly important for people with the damaged skin barrier.
Disclosure of Invention
The object of the first aspect of the present invention is to provide a composition.
The second aspect of the invention aims to provide a composite plant skin barrier protective agent.
The third aspect of the present invention is directed to a method for preparing the composite plant skin barrier protective agent of the second aspect of the present invention.
The fourth aspect of the present invention is directed to the use of the composition of the first aspect of the present invention or the composite plant skin barrier protecting agent of the second aspect of the present invention in the manufacture of a product.
The object of the fifth aspect of the invention is to provide a product.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
in a first aspect of the invention, there is provided a composition comprising the following components: radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract and radix Pulsatillae extract.
Preferably, the composition comprises the following components in parts by weight: 2-8 parts of cynanchum atratum extract, 1-7 parts of bletilla striata extract, 3-10 parts of rhinacanthus nasutus extract, 3-10 parts of willow bark extract and 2-9 parts of pulsatilla extract.
Further preferably, the composition comprises the following components in parts by weight: 4-8 parts of cynanchum atratum extract, 3-6 parts of bletilla striata extract, 4-8 parts of rhinacanthus nasutus extract, 4-8 parts of willow bark extract and 3-7 parts of pulsatilla extract.
Still further preferably, the composition comprises the following components in parts by weight: 5-6 parts of cynanchum atratum extract, 4-5 parts of bletilla striata extract, 5-6 parts of rhinacanthus nasutus extract, 6-7 parts of willow bark extract and 5-6 parts of pulsatilla extract.
The radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract and radix Pulsatillae extract are respectively extracted from radix Cynanchi Atrati, rhizoma Bletillae, Rhinacanthus Nasuta, cortex Salicis Babylonicae and radix Pulsatillae.
Preferably, the cynanchum atratum extract is an aqueous extract of cynanchum atratum.
Preferably, the preparation method of the cynanchum atratum extract comprises the following steps: mixing radix Cynanchi Atrati with water, soaking, crushing, and extracting to obtain radix Cynanchi Atrati extract.
More preferably, the mass ratio of the cynanchum atratum to the water is 1 (4-9).
Further preferably, the soaking time is 1-2 h.
More preferably, the crushing condition is 3000-3500 rpm for 50-70 s.
More preferably, the crushed cynanchum atratum solution is sieved by a sieve of 90-150 meshes.
Further preferably, the extraction condition is that the extraction is carried out for 40-50 min at 80-95 ℃.
Further preferably, the extracted cynanchum atratum solution is cooled to 30-50 ℃, and is sieved by a 500-600-mesh sieve.
More preferably, the pH value of the cynanchum atratum extract is 5.0-8.0, and the viscosity of the cynanchum atratum extract is 200-400 mpa · s.
More preferably, the pH value of the cynanchum atratum extract is 6.0-7.0, and the viscosity of the cynanchum atratum extract is 350-400 mPa & s.
Preferably, the bletilla striata extract is an aqueous extract of bletilla striata.
Preferably, the preparation method of the bletilla striata extract comprises the following steps: mixing rhizoma Bletillae with water, soaking, crushing, and extracting to obtain rhizoma Bletillae extract.
Further preferably, the mass ratio of the bletilla to the water is 1 (5-9).
Further preferably, the soaking time is 0.5-1 h.
More preferably, the crushing condition is 2500-3000 rpm for 20-50 s.
Further preferably, the crushed rhizoma bletillae solution is sieved by a sieve of 90-150 meshes.
Further preferably, the extraction condition is that the extraction is carried out for 40-50 min at 80-95 ℃.
Further preferably, the extracted rhizoma bletillae solution is cooled to 30-50 ℃, and is sieved by a 500-600-mesh sieve.
More preferably, the pH value of the bletilla striata extract is 5.0-8.0, and the viscosity is 200-400 mpa · s.
More preferably, the pH value of the bletilla striata extract is 6.0-7.0, and the viscosity is 290-400 mpa-s.
Preferably, the rhinacanthus nasutus extract is an aqueous extract of rhinacanthus nasutus.
Preferably, the preparation method of the rhinacanthus nasutus extract comprises the following steps: mixing Rhinacanthus nasutus with water, soaking, crushing, and extracting to obtain Rhinacanthus nasutus extract.
Further preferably, the mass ratio of the rhinacanthus nasutus to the water is 1 (3-7).
Further preferably, the soaking time is 20-50 min.
More preferably, the crushing condition is 1500-2500 rpm for 30-50 s.
Preferably, the crushed Rhinacanthus nasutus solution is sieved by a sieve of 90-150 meshes.
Further preferably, the extraction condition is that the extraction is carried out for 40-50 min at 80-95 ℃.
Further preferably, the extracted rhinacanthus nasutus solution is cooled to 30-50 ℃, and is sieved by a 500-600-mesh sieve.
More preferably, the pH value of the rhinacanthus nasutus extract is 5.0-8.0, and the viscosity is 300-450 mpa · s.
More preferably, the pH value of the Rhinacanthus nasutus extract is 6.0-7.0, and the viscosity is 400-450 mpa · s.
Preferably, the white willow bark extract is an aqueous extract of white willow bark.
Preferably, the preparation method of the white willow bark extract comprises the following steps: mixing white willow bark with water, soaking, crushing, and extracting to obtain white willow bark extract.
More preferably, the mass ratio of the white willow bark to the water is 1 (4-10).
Further preferably, the soaking time is 1-2 h.
More preferably, the crushing condition is 2500-4000 rpm for 70-90 s.
Preferably, the crushed white willow bark solution is sieved by a sieve of 90-150 meshes.
Further preferably, the extraction condition is that the extraction is carried out for 40-50 min at 80-95 ℃.
Further preferably, the white willow bark solution after extraction is cooled to 30-50 ℃ and sieved by a 500-600 mesh sieve.
More preferably, the white willow bark extract has a pH of 5.0 to 8.0 and a viscosity of 200 to 400mpa · s.
More preferably, the white willow bark extract has a pH of 6.0 to 7.0 and a viscosity of 300 to 350mPa · s.
Preferably, the pulsatilla chinensis extract is an aqueous extract of pulsatilla chinensis.
Preferably, the preparation method of the pulsatilla chinensis extract comprises the following steps: mixing radix Pulsatillae with water, soaking, crushing, and extracting to obtain radix Pulsatillae extract.
Further preferably, the mass ratio of the Chinese pulsatilla root to the water is 1 (5-8).
Further preferably, the soaking time is 1-2 h, and the soaking temperature is 20-40 ℃.
More preferably, the crushing condition is 3000-4500 rpm for 80-100 s.
Further preferably, the crushed Chinese pulsatilla root solution is sieved by a sieve of 90-150 meshes.
Further preferably, the extraction condition is that the extraction is carried out for 40-50 min at 80-95 ℃.
Further preferably, the extracted Chinese pulsatilla root solution is cooled to 30-50 ℃, and is sieved by a 500-600 mesh sieve.
More preferably, the pH value of the pulsatilla chinensis bunge extract is 5.0-8.0, and the viscosity of the pulsatilla chinensis bunge extract is 200-400 mpa & s.
More preferably, the pH value of the pulsatilla chinensis bunge extract is 6.0-7.0, and the viscosity of the pulsatilla chinensis bunge extract is 350-400 mPa & s.
In a second aspect of the invention, there is provided a composite plant skin barrier protectant comprising a composition according to the first aspect of the invention, sodium metabisulphite, a preservative and a humectant.
Preferably, the composite plant skin barrier protective agent comprises the following components in parts by weight: 11-44 parts of the composition of the first aspect of the invention, 0.1-0.2 part of sodium metabisulfite, 0.08-0.5 part of preservative and 6-12 parts of humectant.
Further preferably, the composite plant skin barrier protective agent comprises the following components in parts by weight: 14-37 parts of the composition of the first aspect of the invention, 0.09-0.2 part of sodium metabisulfite, 0.2-0.5 part of preservative and 7-11 parts of humectant.
Even more preferably. The composite plant skin barrier protective agent comprises the following components in parts by weight: 25-35 parts of the composition of the first aspect of the invention, 0.1-0.2 part of sodium metabisulfite, 0.4-0.5 part of preservative and 8-10 parts of humectant.
Preferably, the preservative is one or more of phenoxyethanol, 1,2 pentanediol, 1,2 hexanediol, sodium dehydroacetate, benzyl alcohol, dibutyl hydroxy toluene, propyl gallic acid, sorbic acid and benzoic acid.
Preferably, the humectant is one or more of glycerin, propylene glycol, butylene glycol, xylitol, trehalose, ethylhexyl glycerin and hyaluronic acid.
Preferably, the composite plant skin barrier protective agent also comprises 60-75 parts of water.
In a third aspect of the present invention, there is provided a method for preparing the composite plant skin barrier protective agent of the second aspect of the present invention, comprising the steps of: mixing water and humectant to obtain solution A; mixing solution a with the composition of the first aspect of the invention and sodium metabisulphite to give solution B; and mixing the solution B with a preservative to obtain the composite plant skin barrier protective agent.
Preferably, the water and the humectant are mixed under the condition of stirring at 20-30 rpm for 5-20 min.
More preferably, the water and the humectant are mixed under the condition of 15-25 rpm for 5-10 min.
Preferably, the solution A is mixed with the composition of the first aspect of the invention and sodium metabisulfite under the condition of stirring at 25-40 rpm for 15-25 min.
Further preferably, the solution A is mixed with the composition of the first aspect of the invention and sodium metabisulfite under the condition of stirring at 30-33 rpm for 15-20 min.
Preferably, the solution B and the preservative are mixed under the condition of stirring at 5-15 rpm for 10-15 min.
Further preferably, the solution B and the preservative are mixed under the condition of stirring at 8-10 rpm for 10-15 min.
Preferably, the solution B is mixed with a preservative and then stands for 0.5-1.5 h.
More preferably, the solution B is mixed with a preservative and then stands for 0.5-1 h.
In a fourth aspect of the invention, there is provided the use of a composition of the first aspect of the invention or a composite plant skin barrier protective agent of the second aspect in any one of (1) to (4);
(1) preparing a cosmetic;
(2) preparing a product for inhibiting water loss of skin;
(3) preparing a product for repairing skin barriers;
(4) preparing the product for promoting the proliferation of the fibroblasts.
In a fifth aspect of the invention, there is provided a product comprising a composition of the first aspect of the invention or a composite plant skin barrier protectant of the second aspect.
The invention has the beneficial effects that:
according to the invention, the cynanchum atratum extract, the bletilla striata extract, the rhinacanthus nasutus extract, the willow bark extract, the pulsatilla chinensis extract and other substances are innovatively and reasonably matched, so that the obtained composition can effectively supplement water and nutrients lost by the skin, maintain the pH value required by cell survival, and especially keep the stable differentiation of stratum corneum cells; the five natural plant extracts are absent and can not cooperate with each other to generate the effect, and the efficacy of the composition can reach the best under the proper proportion.
The components in the composite plant skin barrier protective agent provided by the invention are reasonable in compatibility, have a synergistic effect, promote the increase of lamellar bodies and the transfer of stratum corneum lipid, so that the repair process of the skin stratum corneum barrier is accelerated, the regeneration of cutin can be effectively regulated, the skin cutin barrier is strengthened, and the skin resistance is improved; meanwhile, the composite plant skin barrier protective agent can also promote the regeneration of fibroblasts, improve the repair effect of injured tissues and improve the immunity of the skin.
The raw materials of the composite plant skin barrier protective agent provided by the invention are not added with any hormone component and antibiotic, the natural plant extract is used as the raw material, and the plant extract provides sufficient nutrient components to repair the damaged skin, so that the protective effect is achieved, and the composite plant skin barrier protective agent is mild to the skin, has no stimulation, has no toxic or side effect, and can be suitable for any crowd.
The preparation method of the natural plant extract provided by the invention takes natural plants as raw materials, utilizes a traditional Chinese medicine tissue crusher to rapidly break the walls, extracts and enriches effective active substances in time, avoids the damage or loss of the active substances in the extract caused by high temperature, and can efficiently and stably obtain the plant extract rich in effective active ingredients.
The preparation method of the plant skin barrier protective agent provided by the invention has the advantages of few steps, simple process and safe operation.
Detailed Description
The present invention will now be described in detail with reference to specific examples, but the scope of the present invention is not limited thereto.
The materials, reagents and the like used in the present examples are commercially available materials and reagents unless otherwise specified.
Example 1 preparation of Cynanchum Atrati extract, bletilla striata extract, Rhinacanthus nasuta extract, Salix alba bark extract and Pulsatillae radix extract
The preparation method of the cynanchum atratum extract comprises the following steps: mixing fresh radix Cynanchi Atrati with water at a material-to-liquid ratio of 1:6(w/w), soaking at 30 deg.C for 1.5h, adding into a clean traditional Chinese medicine tissue crusher, crushing at 3000rpm for 60s, and sieving with 100 mesh sieve; putting the filtrate into an extraction tank, heating and extracting at 95 deg.C, keeping the temperature for 45min, circularly cooling to 40 deg.C, and sieving with 500 mesh sieve to obtain radix Cynanchi Atrati extract with pH of 6.5 and viscosity of 380mpa · s at room temperature (25 deg.C).
The preparation method of the bletilla striata extract comprises the following steps: mixing and soaking fresh rhizoma bletilla tuber and water for 1h according to a material-liquid ratio of 1:7(w/w), pouring into a clean traditional Chinese medicine tissue crusher, crushing at 2800rpm for 50s, and sieving with a 100-mesh sieve; putting the filtrate into an extraction tank, heating and extracting at 95 deg.C, keeping the temperature for 45min, circularly cooling to 40 deg.C, and sieving with 500 mesh sieve to obtain rhizoma Bletillae extract with pH of 6.4 and viscosity of 290mpa · s at room temperature (25 deg.C).
The preparation method of the rhinacanthus nasutus extract comprises the following steps: mixing fresh Rhinacanthus nasutus and water according to a material-liquid ratio of 1:4(w/w), soaking for 30min, pouring into a clean traditional Chinese medicine tissue crusher, crushing at 2000rpm for 40s, and sieving with 100 mesh sieve; extracting the filtrate at 95 deg.C for 45min, circularly cooling to 40 deg.C, and sieving with 500 mesh sieve to obtain Rhinacanthus nasutus extract with pH of 6.7 and viscosity of 410mpa · s at room temperature (25 deg.C).
The preparation method of the white willow bark extract comprises the following steps: mixing fresh white willow bark and water according to a material-to-liquid ratio of 1:6(w/w), soaking at 40 deg.C for 1.5h, pouring into a clean traditional Chinese medicine tissue crusher, crushing at 3000rpm for 80s, and sieving with 100 mesh sieve; extracting the filtrate at 95 deg.C for 45min, circularly cooling to 40 deg.C, and sieving with 500 mesh sieve to obtain white willow bark extract with pH of 6.8 and viscosity of 310mpa · s at room temperature (25 deg.C).
The preparation method of the pulsatilla chinensis extract comprises the following steps: mixing fresh radix Pulsatillae and water at a material-to-liquid ratio of 1:6(w/w), soaking at 30 deg.C for 1.5h, pouring into a clean Chinese medicinal tissue crusher, crushing at 4000rpm for 90s, and sieving with 100 mesh sieve; putting the filtrate into an extraction tank, heating and extracting at 95 deg.C, keeping the temperature for 45min, circularly cooling to 40 deg.C, and sieving with 500 mesh sieve to obtain radix Pulsatillae extract with pH of 6.2 and viscosity of 360 mpa.s at room temperature (25 deg.C).
Example 2
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 6 parts of willow bark extract, 5 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 66.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Example 3
A composite plant skin barrier protective agent comprises the following components in parts by weight: 4 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 6 parts of willow bark extract, 5 parts of pulsatilla chinensis extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 67.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Example 4
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 3 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 6 parts of willow bark extract, 5 parts of pulsatilla chinensis extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 67.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Example 5
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 4 parts of rhinacanthus nasutus extract, 6 parts of willow bark extract, 5 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 6 parts of glycerol, 3 parts of propylene glycol and 67.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Example 6
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 5 parts of willow bark extract, 5 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 67.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Example 7
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 6 parts of willow bark extract, 4 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 67.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Example 8
A composite plant skin barrier protective agent comprises the following components in parts by weight: 4 parts of cynanchum atratum extract, 3 parts of bletilla striata extract, 4 parts of rhinacanthus nasutus extract, 5 parts of willow bark extract, 4 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 71.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Example 9
A composite plant skin barrier protective agent comprises the following components in parts by weight: 6 parts of cynanchum atratum extract, 5 parts of bletilla striata extract, 6 parts of rhinacanthus nasutus extract, 7 parts of willow bark extract, 6 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 61.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Comparative example 1
A composite plant skin barrier protective agent comprises the following components in parts by weight: 4 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 6 parts of willow bark extract, 5 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 71.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing to add rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to make it fully stir and dissolve at 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Comparative example 2
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 5 parts of rhinacanthus nasutus extract, 6 parts of willow bark extract, 5 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 71.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, Rhinacanthus nasutus extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Comparative example 3
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 6 parts of willow bark extract, 5 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 71.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing to add radix Cynanchi Atrati extract, rhizoma Bletillae extract, cortex Salicis Babylonicae extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to make it fully stirred and dissolved at 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Comparative example 4
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 5 parts of Chinese pulsatilla root extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 72.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, radix Pulsatillae extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Comparative example 5
A composite plant skin barrier protective agent comprises the following components in parts by weight: 5 parts of cynanchum atratum extract, 4 parts of bletilla striata extract, 5 parts of rhinacanthus nasutus extract, 6 parts of white willow bark extract, 0.2 part of sodium metabisulfite, 0.5 part of phenoxyethanol, 5 parts of glycerol, 3 parts of propylene glycol and 71.3 parts of water;
the preparation method of the composite plant skin barrier protective agent comprises the following steps:
adding water, glycerol and propylene glycol into the sterilized emulsifying pot, and stirring at 20rpm for 5min to uniformly dissolve; continuing adding radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, white willow bark extract and sodium pyrosulfite, and increasing stirring speed of emulsifying pot to fully stir for dissolving, wherein stirring condition is 30rpm for 20 min; adding phenoxyethanol, stirring at 10rpm for 10min, and standing the mixed solution for 0.5h to obtain the composite plant skin barrier protective agent.
Effects of the embodiment
1. Lactic acid irritation test
130 volunteers with 20-35 years old sensitive skin (the face has red swelling and is highly sensitive in a lactic acid irritation experiment test) are selected, randomly divided into 13 groups, 10 persons in each group are respectively smeared on the face by using the composite plant skin barrier protective agent in the examples 2-9 and the comparative examples 1-5, the red swelling fading condition of the face is observed after the composite plant skin barrier protective agent is continuously used for 14 days every morning and evening, and the sensitive skin of each group of volunteers is judged by using the lactic acid irritation experiment.
(1) After 14 days of continuous use of the composite plant skin barrier protective agent of examples 2-9 and comparative examples 1-5, the facial redness and swelling of each volunteer group was observed.
Evaluation standard of facial red and swollen change of volunteers: the effect is shown: over 80 percent of the resolution rate of red swelling; the method has the following advantages: 50-80% of the resolution rate of the red swelling; and (4) invalidation: the resolution of red swelling is 50% or less.
As a result: by observing the change of the facial redness and swelling of each group of volunteers, the results are shown in table 1, the total effective rate of the composite plant skin barrier protective agent of the examples 2-9 is more than 70%, and can reach 100% at most (example 9), while the total effective rate of the composite plant skin barrier protective agent of the comparative examples 1-5 is less than 65%.
(2) Lactic acid irritation test procedure: in the test process, in order to avoid false negative of the test result caused by the fact that the face is coated with the skin care product, the test subject should clean the face with warm water, and the test subject stands for 10-20 min at room temperature (25 ℃) after the face is cleaned, so that false negative caused by transient recovery of the skin barrier due to moisturizing of the face is avoided. The subjects were asked to score subjective symptoms (stinging) at 2.5min and 5min, respectively, and scored on a 4 point scale by applying a 10% lactic acid solution to the nasolabial sulcus and ipsilateral cheek: the test result shows that the individual subject has delayed response, the itching feeling can be shown after 5min, and the test time can be properly prolonged. The two scores were added and divided according to the lactic acid stimulation test into: insensitivity, lactic acid stimulation test 0 min; mild sensitivity, 1-3 minutes of lactic acid stimulation test; moderate sensitivity, 4-5 points of lactic acid stimulation test; high sensitivity, lactic acid stimulation test 6 points.
As a result: the lactic acid irritation test result shows that the total score of the volunteers continuously using the composite plant skin barrier protective agent of the embodiments 2-9 for 14 days is less than 3, which shows that the composite plant skin barrier protective agent of the embodiments 2-9 can effectively improve the repair capability of the epidermis skin, and has the functions of effectively repairing the damaged part and strengthening the barrier function of the skin. In contrast, the total score (> 3 scores) of the volunteers using the composite plant skin barrier protective agent of comparative examples 1-5 in the two lactic acid irritation tests was higher than that of examples 2-9, indicating that five plant extracts such as the cynanchum atratum extract, the bletilla striata extract, the rhinacanthus nasutus extract, the willow bark extract and the pulsatilla root extract in the composite plant skin barrier protective agent are all absent.
TABLE 1 lactic acid irritation test results
2. Evaluation of skin Water loss
The skin moisture loss TEWL is a very important parameter for assessing the function of the skin moisture barrier and has gained wide acceptance internationally. The more intact the skin protection layer, the higher the moisture content and the lower the value of TEWL for skin moisture loss, in units of: g/h m2. In the process of developing cosmetics, the efficacy of the moisturizing cosmetics can be evaluated by testing the numerical value of TEWL (water loss in skin), and the moisturizing cosmetics can also be applied to allergic patch tests, contact dermatitis, physical therapy, burns and monitoring of new tissues to find out whether the protective function of the skin is damaged or not in time.
A skin surface moisture loss tester (Tewameter, TM 300, Courage and Khazaka, Germany) uses a specially designed cylindrical cavity measuring probe with two open ends to form a relatively stable test small environment on the skin surface, and two groups of temperature and humidity sensors are used for measuring the water vapor pressure gradient of the near epidermis (within about 1 cm) formed by the water loss of the horny layer at different points, and the water content evaporated by the epidermis is directly measured, so that the skin surface moisture loss condition is measured.
And (3) testing environmental conditions: the test environment temperature is 26 +/-1 ℃, the humidity is 50% +/-5%, and real-time dynamic monitoring is carried out.
The volunteer requires: people with age of 25-60 years and without serious systemic diseases, immunodeficiency or autoimmune diseases; those with inactive allergic disease; those who have not had a history of severe allergy to skin care cosmetics in the past; hormone drugs and immunosuppressants have not been used in the last 1 month; those who did not participate in other clinical trials; all volunteers should fill out informed consent before testing.
Preparation before testing: any product (cosmetics or external medicine) cannot be used 2-3 days before the tested part. Before formal test, the people should sit still in a room meeting the standard for at least 30min, and cannot drink water to keep relaxed.
Test samples: the composite plant skin barrier protective agent of examples 2 to 9 and comparative examples 1 to 5.
The specific testing steps are as follows: selecting 26 volunteers meeting the requirements, randomly dividing into 13 groups, marking 3X 3cm on the inner side of the left and right arms of the volunteers2Test areas, spaced 1cm apart. Each group of volunteers applied the test sample to the test area on the left and right arms, and the area to be tested was measured using the measuring instrument. The blank value of each test area was measured and then measured at 2.0. + -. 0.2mg of sample/cm2The test samples were applied evenly to the test area and after 1h the TEWL values of the skin were measured in the test area using a TM 300 tester, 3 times in parallel per area.
As a result: as can be seen from Table 2, the TEWL value of the skin water loss in the test area after the plant composite skin barrier protective agent of examples 2-9 is used is 10-13.5 g/hm2Is obviously lower than the TEWL value (more than 25g/h m) of the skin water loss after the plant composite skin barrier protective agent of the comparative examples 1-5 is used2) The results show that the plant composite skin barrier protective agent of examples 2-9 can significantly reduce the loss of skin moisture, enhance the internal cell water circulation, strengthen the surface water-locking barrier, and achieve the effect of repairing the skin barrier, and the achieved effect is significantly better than that of a sample lacking some components.
TABLE 2 skin Water loss TEWL values
3. Test for stratum corneum Barrier repair Effect
The effect of the composite plant skin barrier protective agent of examples 2-9 and the composite plant skin barrier protective agent of comparative examples 1-5 on stratum corneum barrier repair was examined by skin lamellar body repair.
The experimental principle is as follows: when the skin barrier is acutely damaged, the stratum granulosum cells quickly secrete and form precursor lamellar bodies, so that the quantity of the lamellar bodies in a short term is reduced, lipid transfer is inhibited, and the primary repair of the stratum corneum lipid membrane is achieved. The newly generated lamellar bodies then appear in the granular layer and act rapidly until the permeability barrier function is restored.
The experimental method comprises the following steps: 20 volunteers without active skin diseases were recruited in the experiment, and before the experiment, the volunteers cleaned both forearms with clear water and sat for 30min in an environment with a temperature of 24 + -1 ℃ and a relative humidity of 55 + -5%. Selecting 14 parts of the forearm flexor side of a volunteer, wherein each part is 1cm multiplied by 1cm in size, carrying out tape pasting (the tape pasting not only reduces horny cells and intercellular lipid of horny layer, but also thins the horny layer to cause mechanical damage to skin barrier), pasting each part for 15 times, keeping the pasting time, pressure and tape tearing speed and direction consistent, and recording the quantity L of lamellar bodies at the moment0. Spreading test sample composite plant skin barrier protective agent on 13 parts, using the rest part as blank control without spreading anything, and recording the number L of lamellar bodies after 30min1. Wherein the laminated body has a rate of change of [ L ]1-L0)/L0]×100％。
As can be seen from table 3, the composite plant skin barrier protective agent of examples 2 to 9 has an obvious barrier repairing effect on the skin cuticle compared with the composite plant skin barrier protective agent of comparative examples 1 to 5, the change rate of lamellar bodies is greater than 28%, the increase of lamellar bodies is promoted, the repairing process of the barrier is accelerated, which indicates that the components in the composite plant skin barrier protective agent of examples 2 to 9 are reasonably compatible, and the composite plant skin barrier protective agent has a synergistic effect, promotes the increase of lamellar bodies, promotes the transportation of cuticle lipid, accelerates the repairing process of the skin cuticle barrier, and can effectively regulate the regeneration of cuticle, strengthen the skin cuticle barrier, and improve the skin resistance.
Table 3 stratum corneum barrier repair effect test results
4. Fibroblast proliferation Activity assay
The effects of the composite plant skin barrier protective agents of examples 2-9 and the composite plant skin barrier protective agents of comparative examples 1-5 were examined by a fibroblast proliferation activity test.
The experimental process comprises the following steps: the composite plant skin barrier protective agent of examples 2-9 and the composite plant skin barrier protective agent of comparative examples 1-5 were dissolved in dimethyl sulfoxide (mass ratio of the composite plant skin barrier protective agent to the dimethyl sulfoxide is 1:2), filtered and sterilized with a 0.2mm sterile filter head, and then prepared into a culture medium containing 10% of the composite plant skin barrier protective agent with a DMEM culture solution. Obtaining human skin fibroblast by tissue block culture method, adding 1ml 0.25% trypsin for digestion, centrifuging, re-suspending with DMEM culture solution, counting and diluting to cell concentration of 1 × 105Per mL; inoculating into a 96-well plate, wherein each well is 100mL, and culturing for 48 h; observing cell adherence under microscope, sucking out culture medium in 96-well plate, adding culture medium containing 10% composite plant skin barrier protectant, and culturing at 37 deg.C and 5% CO2Standing and culturing for 24h and 48h in an incubator; after the culture was completed, 4 wells were each prepared, and 20mL of MTT solution was added to each well, followed by CO2Continuously incubating for 4h in the incubator; 150mL of dimethyl sulfoxide is added into each hole, the mixture is placed on a constant temperature shaking table to be oscillated at low speed for 10min, and then the light absorption value of each hole is measured at 490nm on an enzyme labeling instrument (the higher the light absorption value is, the better the effect on promoting cell regeneration is, the better the repairing effect of injured tissues can be promoted, and immune cells can promote chronic wound repair or skin regeneration).
TABLE 4 fibroblast proliferation Activity test results
The results are shown in table 4, after 48 hours of culture, the absorbance values (0.798-0.928) of the composite plant skin barrier protective agents of examples 2-9 are obviously greater than the absorbance values (0.334-0.412) of the composite plant skin barrier protective agents of comparative examples 1-5, and it can be seen that the composite plant skin barrier protective agents of examples 2-9 can effectively promote the regeneration effect of fibroblasts, and the repair effect of the injured tissues is better as time goes on, which indicates that the components in the composite plant skin barrier protective agents of examples 2-9 are reasonably compatible, have a synergistic effect, have a good effect of promoting the regeneration of cells, can remarkably promote the repair effect of the injured tissues, and improve the immunity of the skin.
The embodiments of the present invention have been described in detail, but the present invention is not limited to the embodiments, and various changes can be made without departing from the gist of the present invention within the knowledge of those skilled in the art. Furthermore, the embodiments of the present invention and the features of the embodiments may be combined with each other without conflict.
Claims (10)
1. A composition comprising the following components: radix Cynanchi Atrati extract, rhizoma Bletillae extract, Rhinacanthus Nasuta extract, cortex Salicis Babylonicae extract and radix Pulsatillae extract.
2. The composition according to claim 1, characterized in that it comprises the following components in parts by weight: 2-8 parts of cynanchum atratum extract, 1-7 parts of bletilla striata extract, 3-10 parts of rhinacanthus nasutus extract, 3-10 parts of willow bark extract and 2-9 parts of pulsatilla extract.
3. The composition according to claim 1 or 2, wherein the extracts of cynanchum atratum, bletilla striata, rhinacanthus nasutus, willow bark and pulsatilla chinensis are respectively extracted from cynanchum atratum, bletilla striata, rhinacanthus nasutus, willow bark and pulsatilla chinensis.
4. A skin barrier protectant comprising the composition of claim 1, sodium metabisulfite, a preservative, and a humectant.
5. The skin barrier protectant according to claim 4, comprising the following components in parts by weight: 11 to 44 parts of the composition of claim 1, 0.08 to 0.2 part of sodium metabisulfite, 0.1 to 0.5 part of preservative and 6 to 12 parts of humectant.
6. The skin barrier protectant according to claim 5, comprising the following components in parts by weight: 14-37 parts of the composition as claimed in claim 1, 0.09-0.2 part of sodium metabisulfite, 0.2-0.5 part of preservative and 7-11 parts of humectant.
7. A process for the preparation of a skin barrier protectant according to any of claims 4 to 6, comprising the steps of: mixing water and humectant to obtain solution A; mixing solution a with the composition of claim 1 and sodium metabisulfite to obtain solution B; mixing the solution B with antiseptic to obtain skin barrier protectant.
8. The method according to claim 7, wherein the solution A is mixed with the composition of claim 1 and sodium metabisulfite under the condition of stirring at 25-40 rpm for 15-25 min.
9. Use of the composition of any one of claims 1 to 3 or the skin barrier protecting agent of any one of claims 4 to 6 in any one of (1) to (4);
(1) preparing a cosmetic;
(2) preparing a product for inhibiting water loss of skin;
(3) preparing a product for repairing skin barriers;
(4) preparing the product for promoting the proliferation of the fibroblasts.
10. A product comprising the composition of any one of claims 1 to 3 or the skin barrier protectant of any one of claims 4 to 6.
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| CN111870575A (en) * | 2020-08-24 | 2020-11-03 | 广州三广生物科技有限公司 | Traditional Chinese medicine composition for whitening and removing freckles and preparation method thereof |
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| CN101467941A (en) * | 2007-12-27 | 2009-07-01 | 天津天阜康生物技术发展有限公司 | Chinese medicine mask with whitening and spot-eliminating functions and technique for preparing the same |
| CN112402332A (en) * | 2019-08-22 | 2021-02-26 | 陈伟琼 | Proportional components of European lyre-hibiscus ganoderma lucidum honey turbidity-expelling and face-beautifying cream and preparation method thereof |
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