CN114292722B - Strong leakproofness PCR shrouding membrane - Google Patents

Strong leakproofness PCR shrouding membrane Download PDF

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Publication number
CN114292722B
CN114292722B CN202111460958.5A CN202111460958A CN114292722B CN 114292722 B CN114292722 B CN 114292722B CN 202111460958 A CN202111460958 A CN 202111460958A CN 114292722 B CN114292722 B CN 114292722B
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plate
mounting
limiting
sealing
pcr
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CN114292722A (en
Inventor
刘子德
刘子贤
刘刚
袁义勇
赵华
唐波
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Meidong Huicheng Life Technology Kunshan Co ltd
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Meidong Huicheng Life Technology Kunshan Co ltd
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Priority to CN202111460958.5A priority Critical patent/CN114292722B/en
Publication of CN114292722A publication Critical patent/CN114292722A/en
Priority to PCT/CN2022/134589 priority patent/WO2023098604A1/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Package Closures (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Closures For Containers (AREA)

Abstract

The invention provides a strong-tightness PCR sealing plate film, which relates to the technical field of experimental equipment and comprises a bearing plate, wherein the lower surface of the bearing plate is provided with a sealing plate film main body, the upper surface of the bearing plate is fixedly provided with a mounting shell, the center of the upper surface of the mounting shell is provided with an adjusting mechanism, an inner partition plate is arranged in the mounting shell, a first limiting mechanism is arranged in the mounting shell and above the inner partition plate, and a second limiting mechanism is arranged in the mounting shell and below the inner partition plate. According to the invention, through the cooperation between the first clamping block and the clamping groove, the pressing rod can push the second connecting rod to move, so that the safety limit baffle can move downwards, the periphery of the sealing plate film main body and the periphery of the PCR plate can be sealed, the limit baffle can be better attached to the sealing plate film main body, and the sealing effect of the sealing plate film main body is better.

Description

Strong leakproofness PCR shrouding membrane
Technical Field
The invention relates to the technical field of experimental equipment, in particular to a strong-tightness PCR sealing plate membrane.
Background
The PCR plate is a container for placing some specific experimental reactants, and the PCR plate needs a sealing plate film to seal the container in the transportation and storage process, as a sealing plate film for a PCR reaction plate provided in the patent with the application number of 202022266520.0, a sealing plate film for a PCR reaction plate comprises a polymer sheet, the polymer sheet is provided with a plurality of vertically through convex columns, the openings of the convex columns penetrate through the polymer sheet, and a polymer transparent film made of transparent materials is laid on one side of the polymer sheet far away from the convex columns. By adopting the technical scheme, the pollution of the sealing plate film adhesive to the template is reduced, and the sealing plate film adhesive has better ultraviolet transmittance and better heat resistance; meanwhile, the connecting structure is not required to be too complex, and the structure is simple.
But foretell technical scheme shrouding membrane is relatively poor to the leakproofness of PCR board, leads to the experiment reactant in the PCR board to receive external factor influence easily to cause pollution damage, and then influences the accuracy of experimental result, produces the position deviation easily when laminating moreover, and is comparatively inconvenient during the use, so we do the improvement to this, propose a strong leakproofness PCR shrouding membrane.
Disclosure of Invention
(one) solving the technical problems
Aiming at the defects of the prior art, the invention provides a strong-tightness PCR sealing plate film, which solves the problems that reactants are easy to be polluted and damaged due to poor sealing performance of the sealing plate film and position deviation is easy to generate during lamination.
(II) technical scheme
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the utility model provides a strong leakproofness PCR shrouding membrane, includes the loading board, the lower surface of loading board is provided with shrouding membrane main part, the lower surface evenly distributed of shrouding membrane main part is provided with a plurality of sealed lid, every sealed lower surface of lid all is provided with sealed post, every the leading-in spacing ring is all installed to sealed bottom of post, the upper surface fixed of loading board is provided with the installation casing, the upper surface center department of installation casing is provided with adjustment mechanism, the internally mounted of installation casing has interior baffle, the inside of installation casing just is provided with first stop gear in the top of baffle, the inside of installation casing just is provided with second stop gear in the below of baffle.
Preferably, the adjusting mechanism comprises a pressing rod and a movable mounting block, wherein the pressing rod is movably arranged at the center of the upper surface of the mounting shell, and the lower surface of the movable mounting block is fixedly provided with a mounting sliding block.
Preferably, the installation slider slides and sets up in spacing spout, the both ends surface of removal installation piece all is provided with auxiliary push rod, the one side surface fixed mounting who removes the installation piece and be close to the press pole has the gag lever post.
Preferably, the pressing rod is uniformly provided with a plurality of limiting holes, a limiting plate is arranged on the upper surface of the installation shell and positioned on one side far away from the movable installation block, and a first clamping block is fixedly arranged on one end surface of the pressing rod positioned inside the installation shell.
Preferably, a first mounting plate is fixedly mounted on the surface of one side of the pressing rod, which is positioned in the mounting shell, a first spring is arranged on the lower surface of the first mounting plate, and the other end of the first spring is fixedly mounted on the upper surface of the inner partition plate.
Preferably, the first limiting mechanism comprises a first connecting plate and a second mounting plate, the first connecting plate is fixedly mounted on the surfaces of two ends of the pressing rod, which are located in the mounting shell, and the second mounting plate is fixedly mounted on the surface of one end of the first connecting plate, which is far away from the pressing rod.
Preferably, first connecting rods are fixedly arranged on two sides of the lower surface of the second mounting plate, the other end of each first connecting rod penetrates through the inner partition plate, a second clamping block is fixedly arranged at the end part of each first connecting rod, and first guide parts are arranged on two sides of the bottom of each second clamping block.
Preferably, four mounting grooves are uniformly distributed on the upper surface of the bearing plate, second springs are fixedly mounted on the inner walls of two ends of each mounting groove, limiting clamping plates are fixedly mounted on the other ends of each second spring, second guide portions are arranged on one side of the top of each limiting clamping plate, and the limiting clamping plates are arranged below the second clamping blocks.
Preferably, the second limiting mechanism comprises a second connecting rod and a clamping groove, the second connecting rod is movably arranged at the center of the inner partition plate, the clamping groove is formed in the top surface of the second connecting rod, the second connecting plate is fixedly arranged at one end part of the second connecting rod below the inner partition plate, a plurality of third springs are uniformly distributed on the upper surface of the second connecting plate, and the other ends of the third springs are fixedly arranged on the lower surface of the inner partition plate.
Preferably, the end part of the second connecting plate far away from the second connecting rod is fixedly provided with a limit baffle, one side of the bottom of the limit baffle is provided with an end part limiting block, the limit baffle is arranged in an installation limit groove, and the installation limit groove is arranged in the bearing plate and is positioned at the outer side of the sealing plate film main body.
Compared with the prior art, the invention has the following beneficial effects:
1. through the cooperation between first fixture block and the draw-in groove, can make the push rod promote the second connecting rod and remove, and then can make the limit baffle of installation spacing inslot move down, make limit baffle can seal the shrouding membrane main part and the PCR board all around, through setting up the tip stopper in limit baffle bottom, can make and can carry out better laminating between limit baffle and the shrouding membrane main part, make the sealed effect of shrouding membrane main part better, make the inside difficult external factor that receives of PCR board pollute, can make the testing result more accurate.
2. The pressing rod can drive the first connecting plate of its both sides to remove at the in-process of downwardly moving, can make second fixture block and mounting groove cooperate under the head rod effect, under the effect of having two spacing splint through second spring movable mounting in the mounting groove, make two spacing splint spacing to the position chucking of second fixture block, can make laminating effect between shrouding membrane main part and the PCR board better, can not produce great positional deviation at the laminating in-process, more facilitate the use.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a strong-tightness PCR sealing plate membrane of the invention;
FIG. 2 is a schematic diagram showing a main structure of a sealing plate film of the strong-sealing PCR sealing plate film;
FIG. 3 is a schematic view of another angular configuration of a seal plate membrane body of a strong-tightness PCR seal plate membrane according to the present invention;
FIG. 4 is an enlarged view of the portion A of FIG. 1 of a strong seal PCR closure membrane according to the present invention;
FIG. 5 is an enlarged view of the portion B of FIG. 3 of a strong seal PCR closure membrane according to the present invention;
FIG. 6 is a front view of a strong seal PCR seal plate membrane of the present invention;
FIG. 7 is a schematic cross-sectional view of the strong seal PCR closure membrane of FIG. 6 at C-C;
FIG. 8 is a schematic cross-sectional view of the strong seal PCR closure membrane of FIG. 6 at D-D;
FIG. 9 is a schematic cross-sectional view of the strong seal PCR closure membrane of FIG. 6 at E-E;
FIG. 10 is an enlarged view of the portion F of FIG. 8 of a strong seal PCR closure membrane according to the present invention.
In the figure: 1. a carrying plate; 2. a seal plate film body; 201. sealing cover; 202. a sealing column; 203. leading in a limiting ring; 3. a mounting shell; 4. an adjusting mechanism; 401. pressing a pressing rod; 402. moving the mounting block; 403. installing a sliding block; 404. limiting sliding grooves; 405. an auxiliary push rod; 406. a limit rod; 407. a limiting hole; 408. a limiting plate; 409. a first clamping block; 410. a first mounting plate; 411. a first spring; 5. an inner partition; 6. a first limiting mechanism; 601. a first connection plate; 602. a second mounting plate; 603. a first connecting rod; 604. a second clamping block; 605. a first guide part; 606. a mounting groove; 607. a second spring; 608. limiting clamping plates; 609. a second guide part; 7. a second limiting mechanism; 701. a second connecting rod; 702. a clamping groove; 703. a second connecting plate; 704. a third spring; 705. a limit baffle; 706. an end limiting block; 707. and installing a limit groove.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Examples
As shown in fig. 1-10, a strong-tightness PCR sealing plate film comprises a bearing plate 1, wherein a sealing plate film main body 2 is arranged on the lower surface of the bearing plate 1, a plurality of sealing covers 201 are uniformly distributed on the lower surface of the sealing plate film main body 2, sealing columns 202 are arranged on the lower surface of each sealing cover 201, a leading-in limiting ring 203 is arranged at the bottom of each sealing column 202, an installation shell 3 is fixedly arranged on the upper surface of the bearing plate 1, an adjusting mechanism 4 is arranged at the center of the upper surface of the installation shell 3, an inner partition plate 5 is arranged in the installation shell 3, a first limiting mechanism 6 is arranged in the installation shell 3 and above the inner partition plate 5, and a second limiting mechanism 7 is arranged in the installation shell 3 and below the inner partition plate 5.
In this embodiment, the adjustment mechanism 4 includes a pressing rod 401 and a movable mounting block 402, the pressing rod 401 is movably disposed at the center of the upper surface of the mounting housing 3, a mounting sliding block 403 is fixedly mounted on the lower surface of the movable mounting block 402, the mounting sliding block 403 is slidably disposed in a limiting sliding groove 404, auxiliary pushing rods 405 are disposed on two end surfaces of the movable mounting block 402, a limiting rod 406 is fixedly mounted on one side surface of the movable mounting block 402, which is close to the pressing rod 401, and the movable mounting block 402 is slidably mounted through the mounting sliding block 403 slidably disposed in the limiting sliding groove 404, so that the limiting rod 406 is more convenient for adjustment and use.
It should be noted that, a plurality of limiting holes 407 are uniformly distributed on the pressing rod 401, a limiting plate 408 is arranged on the upper surface of the installation shell 3 and on one side far away from the movable installation block 402, a first clamping block 409 is fixedly arranged on one end surface of the pressing rod 401 inside the installation shell 3, and the position of the limiting rod 406 can be limited when the limiting rod 406 passes through the limiting holes 407 through the arrangement of the limiting plate 408, so that the position of the limiting rod 406 is not easy to change, and the fixing effect of the pressing rod 401 is better.
When specifically setting up, the pressure pole 401 is located the inside one side surface fixed mounting of installation casing 3 and has first mounting panel 410, and the lower surface of first mounting panel 410 is provided with first spring 411, and the other end fixed mounting of first spring 411 is at the upper surface of inner separator 5, under the elastic action of first spring 411, can accomplish the work that resets after pressing down pressure pole 401, and is more convenient when using.
The first limiting mechanism 6 comprises a first connecting plate 601 and a second mounting plate 602, the first connecting plate 601 is fixedly mounted on two end surfaces of the pressing rod 401 inside the mounting shell 3, the second mounting plate 602 is fixedly mounted on one end surface of the first connecting plate 601, which is far away from the pressing rod 401, the first connecting rods 603 are fixedly mounted on two sides of the lower surface of the second mounting plate 602, the other end of each first connecting rod 603 penetrates through the inner partition 5 and the end of each second connecting rod 604 to form a second clamping block 604, the first guiding parts 605 are arranged on two sides of the bottom of each second clamping block 604, four mounting grooves 606 are uniformly distributed on the upper surface of the bearing plate 1, second springs 607 are fixedly mounted on two end inner walls of each mounting groove 606, limiting clamp plates 608 are fixedly mounted on the other end of each second spring 607, second guiding parts 609 are arranged on one side of the top of each limiting clamp plate 608, and the limiting clamp plates 608 are arranged under the second clamping blocks 604.
It should be noted that, the second stop mechanism 7 includes a second connecting rod 701 and a clamping groove 702, the second connecting rod 701 is movably mounted at the center of the inner partition 5, the clamping groove 702 is provided on the top surface of the second connecting rod 701, the second connecting rod 703 is fixedly mounted at one end of the second connecting rod 701 below the inner partition 5, a plurality of third springs 704 are uniformly distributed on the upper surface of the second connecting rod 703, the other end of the third springs 704 is fixedly mounted at the lower surface of the inner partition 5, the end of the second connecting rod 703 far away from the second connecting rod 701 is fixedly mounted with a stop plate 705, an end stop block 706 is provided at one side of the bottom of the stop plate 705, the stop plate 705 is disposed in the mounting and limiting groove 707, the mounting and limiting groove 707 is disposed in the carrier plate 1 and is located at the outer side of the sealing plate film main body 2, the pressing rod 401 can push the second connecting rod 701 to move through the cooperation between the first clamping block 409 and the clamping groove 702, and the stop plate 705 can seal the periphery of the sealing plate film main body 2 and the PCR plate, and the end stop block 706 can enable the stop plate 705 to be better bonded with the sealing plate main body 2.
The working principle of the strong-tightness PCR sealing plate film is as follows:
when in use, the sealing plate film main body 2 is packaged on the PCR plate, each hole on the PCR plate can be independently sealed by arranging a plurality of sealing columns 202, the size of the leading-in limiting ring 203 is slightly larger than that of the sealing columns 202, the sealing columns 202 are not easy to pop up when external force is received, the upper end of each sealing column 202 is provided with a sealing cover 201, the sealing effect is better, when the packaging of the sealing plate film main body 2 is completed, the first clamping block 409 and the clamping groove 702 can be matched by pressing the pressing rod 401 downwards, the pressing rod 401 can further push the second connecting rod 701 downwards, when the second connecting rod 701 downwards moves, the second connecting rod 703 downwards moves, the limiting baffle 705 in the mounting limiting groove 707 downwards, the limiting baffle 705 can seal the periphery of the sealing plate film main body 2 and the PCR plate, and the end limiting block 706 arranged at the bottom of the limiting baffle 705, the limit baffle 705 can be better attached to the sealing plate film main body 2, the sealing effect of the sealing plate film main body 2 is better, the inside of the PCR plate is not easy to be polluted by external factors, the detection result is more accurate, the pressing rod 401 can drive the first connecting plates 601 at two sides of the pressing rod to move in the downward moving process, the first connecting rod 603 arranged on the lower surface of the second mounting plate 602 can move, the second clamping block 604 at the bottom of the first connecting rod 603 can be matched with the mounting groove 606, the two limit clamping plates 608 are movably arranged in the mounting groove 606 through the second spring 607, the positions of the two limit clamping plates 608 clamp and limit the positions of the second clamping blocks 604 under the elastic action of the spring, the attaching effect between the sealing plate film main body 2 and the PCR plate is better, and larger position deviation can not be generated in the attaching process, more conveniently use, after pressing the pole 401 to the assigned position, promote through auxiliary push rod 405 and remove installation piece 402 and remove to make gag lever post 406 pass the spacing hole 407 of assignment on the pole 401 of pressing, and then can carry out spacing fixed to the position of pressing the pole 401, thereby can make first stop gear 6 and second stop gear 7 more firm to the position definition of shrouding membrane main part 2, make shrouding membrane main part 2's sealed effect better.
The foregoing has shown and described the basic principles and main features of the present invention and the advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (4)

1. The utility model provides a strong leakproofness PCR shrouding membrane, includes loading board (1), its characterized in that: the bearing plate comprises a bearing plate body (1), wherein a sealing plate film main body (2) is arranged on the lower surface of the bearing plate body (1), a plurality of sealing covers (201) are uniformly distributed on the lower surface of the sealing plate film main body (2), sealing columns (202) are arranged on the lower surface of each sealing cover (201), a guide-in limiting ring (203) is arranged at the bottom of each sealing column (202), an installation shell (3) is fixedly arranged on the upper surface of the bearing plate (1), an adjusting mechanism (4) is arranged at the center of the upper surface of the installation shell (3), an inner partition plate (5) is arranged in the installation shell (3), a first limiting mechanism (6) is arranged in the installation shell (3) and above the inner partition plate (5), and a second limiting mechanism (7) is arranged in the installation shell (3) and below the inner partition plate (5); the adjusting mechanism (4) comprises a pressing rod (401) and a movable mounting block (402), the pressing rod (401) is movably arranged at the center of the upper surface of the mounting shell (3), a mounting sliding block (403) is fixedly arranged on the lower surface of the movable mounting block (402), the mounting sliding block (403) is slidably arranged in a limiting sliding groove (404), auxiliary pushing rods (405) are arranged on the two end surfaces of the movable mounting block (402), a limiting rod (406) is fixedly arranged on one side surface of the movable mounting block (402) close to the pressing rod (401), a plurality of limiting holes (407) are uniformly distributed on the pressing rod (401), a limiting plate (408) is arranged on the upper surface of the mounting shell (3) and on one side far away from the movable mounting block (402), a first clamping block (409) is fixedly arranged on one end surface of the pressing rod (411) positioned inside the mounting shell (3), a first mounting plate (410) is fixedly arranged on one side surface of the pressing rod (401) positioned inside the mounting shell (3), a first mounting plate (410) is uniformly distributed on one end surface of the first mounting plate (410), and a first spring (5) is fixedly arranged on the other end surface of the first mounting plate (5); the second stop gear (7) is including second connecting rod (701) and draw-in groove (702), the center department of baffle (5) including second connecting rod (701) movable mounting, the top surface of second connecting rod (701) is provided with draw-in groove (702), one end tip fixed mounting that second connecting rod (701) are located interior baffle (5) below has second connecting plate (703), the upper surface evenly distributed of second connecting plate (703) is provided with a plurality of third spring (704), the lower surface of baffle (5) including the other end fixed mounting of third spring (704), tip fixed mounting that second connecting plate (703) kept away from second connecting rod (701) has limit baffle (705), limit baffle (705) bottom one side is provided with tip stopper (706), limit baffle (705) set up in installation limit groove (707), install limit groove (707) set up in loading board (1) and be located the outside of shrouding main part (2).
2. The strong-tightness PCR sealing plate film as claimed in claim 1, wherein: the first limiting mechanism (6) comprises a first connecting plate (601) and a second mounting plate (602), the first connecting plate (601) is fixedly mounted on two end surfaces of the pressing rod (401) located inside the mounting shell (3), and the second mounting plate (602) is fixedly mounted on one end surface of the first connecting plate (601) away from the pressing rod (401).
3. A strong seal PCR sealing plate membrane according to claim 2, wherein: first connecting rods (603) are fixedly arranged on two sides of the lower surface of the second mounting plate (602), the other end of each first connecting rod (603) penetrates through the inner partition plate (5), a second clamping block (604) is fixedly arranged at the end of each first connecting rod, and first guide parts (605) are arranged on two sides of the bottom of each second clamping block (604).
4. A strong seal PCR sealing plate membrane according to claim 3, wherein: four mounting grooves (606) are uniformly distributed in the upper surface of the bearing plate (1), second springs (607) are fixedly mounted on the inner walls of two ends of each mounting groove (606), limiting clamping plates (608) are fixedly mounted on the other ends of each second spring (607), second guide portions (609) are arranged on one side of the top of each limiting clamping plate (608), and each limiting clamping plate (608) is arranged below each second clamping block (604).
CN202111460958.5A 2021-12-02 2021-12-02 Strong leakproofness PCR shrouding membrane Active CN114292722B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202111460958.5A CN114292722B (en) 2021-12-02 2021-12-02 Strong leakproofness PCR shrouding membrane
PCT/CN2022/134589 WO2023098604A1 (en) 2021-12-02 2022-11-28 Pcr plate sealing film having high sealing performance

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111460958.5A CN114292722B (en) 2021-12-02 2021-12-02 Strong leakproofness PCR shrouding membrane

Publications (2)

Publication Number Publication Date
CN114292722A CN114292722A (en) 2022-04-08
CN114292722B true CN114292722B (en) 2023-10-10

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CN202111460958.5A Active CN114292722B (en) 2021-12-02 2021-12-02 Strong leakproofness PCR shrouding membrane

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WO (1) WO2023098604A1 (en)

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CN114292722B (en) * 2021-12-02 2023-10-10 美东汇成生命科技(昆山)有限公司 Strong leakproofness PCR shrouding membrane

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