CN114292722A

CN114292722A - PCR (polymerase chain reaction) sealing plate film with strong sealing property

Info

Publication number: CN114292722A
Application number: CN202111460958.5A
Authority: CN
Inventors: 刘子德; 刘子贤; 刘刚; 袁义勇; 赵华; 唐波
Original assignee: Meidong Huicheng Life Technology Kunshan Co ltd
Current assignee: Meidong Huicheng Life Technology Kunshan Co ltd
Priority date: 2021-12-02
Filing date: 2021-12-02
Publication date: 2022-04-08
Anticipated expiration: 2041-12-02
Also published as: WO2023098604A1; CN114292722B

Abstract

The invention provides a high-sealing PCR (polymerase chain reaction) sealing plate membrane, which relates to the technical field of experimental equipment and comprises a bearing plate, wherein a sealing plate membrane main body is arranged on the lower surface of the bearing plate, an installation shell is fixedly arranged on the upper surface of the bearing plate, an adjusting mechanism is arranged at the center of the upper surface of the installation shell, an inner partition plate is arranged in the installation shell, a first limiting mechanism is arranged in the installation shell and above the inner partition plate, and a second limiting mechanism is arranged in the installation shell and below the inner partition plate. According to the invention, through the matching between the first clamping block and the clamping groove, the pressing rod can push the second connecting rod to move, so that the mounting limit baffle can move downwards, the periphery of the sealing plate film main body and the periphery of the PCR plate can be sealed, the limit baffle can be better attached to the sealing plate film main body, and the sealing effect of the sealing plate film main body is better.

Description

PCR (polymerase chain reaction) sealing plate film with strong sealing property

Technical Field

The invention relates to the technical field of experimental equipment, in particular to a PCR (polymerase chain reaction) sealing plate film with strong sealing property.

Background

The PCR plate is a container for placing some specific experimental reactants, and the PCR plate needs a sealing plate film to seal the PCR plate in the transportation and storage processes, for example, a sealing plate film for the PCR plate, which is proposed by the patent with the application number of 202022266520.0, the sealing plate film for the PCR plate comprises a polymer sheet, the polymer sheet is provided with a plurality of up-and-down through convex columns, the openings of the convex columns penetrate through the polymer sheet, and a polymer transparent film made of a transparent material is laid on one side of the polymer sheet, which is far away from the convex columns. By adopting the technical scheme, the pollution of the sealing plate film adhesive to the template is reduced, and the template has better ultraviolet transmittance and better heat resistance; meanwhile, an excessively complex connecting structure is not required to be arranged, and the structure is simple.

However, the sealing performance of the sealing plate film on the PCR plate is poor, so that the experimental reactants in the PCR plate are easily affected by external factors to cause pollution and damage, the accuracy of the experimental result is affected, position deviation is easily generated during laminating, and the use is inconvenient.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides the PCR plate sealing membrane with strong sealing property, and solves the problems that reactants are easily polluted and damaged and position deviation is easily generated during the lamination due to poor sealing property of the plate sealing membrane.

(II) technical scheme

In order to achieve the purpose, the invention adopts the technical scheme that:

the utility model provides a strong leakproofness PCR shrouding membrane, includes the loading board, the lower surface of loading board is provided with shrouding membrane main part, the lower surface evenly distributed of shrouding membrane main part is provided with the sealed lid of a plurality of, every the lower surface of sealed lid all is provided with sealed post, every leading-in spacing ring is all installed to the bottom of sealed post, the last fixed surface of loading board is provided with the installation casing, the upper surface center department of installation casing is provided with adjustment mechanism, the internally mounted of installation casing has the inner baffle, the inside of installation casing and the top that is located the inner baffle are provided with first stop gear, the inside of installation casing and the below that is located the inner baffle are provided with second stop gear.

Preferably, the adjusting mechanism comprises a pressing rod and a movable mounting block, the pressing rod is movably arranged at the center of the upper surface of the mounting shell, and a mounting sliding block is fixedly mounted on the lower surface of the movable mounting block.

Preferably, the mounting sliding block is slidably disposed in the limiting sliding groove, the two end surfaces of the movable mounting block are provided with auxiliary push rods, and a limiting rod is fixedly mounted on the surface of one side, close to the pressing rod, of the movable mounting block.

Preferably, a plurality of limiting holes are formed in the pressing rod in an evenly distributed mode, a limiting plate is arranged on the upper surface of the installation shell and on one side, away from the movable installation block, of the upper surface of the installation shell, and a first clamping block is fixedly installed on the surface of one end, located inside the installation shell, of the pressing rod.

Preferably, a first mounting plate is fixedly mounted on one side surface of the pressing rod inside the mounting shell, a first spring is arranged on the lower surface of the first mounting plate, and the other end of the first spring is fixedly mounted on the upper surface of the inner partition plate.

Preferably, the first limiting mechanism comprises a first connecting plate and a second mounting plate, the first connecting plate is fixedly mounted on the two end surfaces of the pressing rod inside the mounting shell, and the second mounting plate is fixedly mounted on the end surface of the first connecting plate far away from the pressing rod.

Preferably, first connecting rods are fixedly mounted on two sides of the lower surface of the second mounting plate, the other end of each first connecting rod penetrates through the inner partition plate, a second clamping block is fixedly mounted at the end of each first connecting rod, and first guide portions are arranged on two sides of the bottom of each second clamping block.

Preferably, the upper surface evenly distributed of loading board is provided with four mounting grooves, every the both ends inner wall fixed mounting of mounting groove has the second spring, every the other end of second spring all fixed mounting has spacing splint, every top one side of spacing splint all is provided with the second guide part, spacing splint set up the below at the second fixture block.

Preferably, the second limiting mechanism comprises a second connecting rod and a clamping groove, the second connecting rod is movably mounted at the center of the inner partition plate, the clamping groove is formed in the top surface of the second connecting rod, a second connecting plate is fixedly mounted at one end, located below the inner partition plate, of the second connecting rod, a plurality of third springs are uniformly distributed on the upper surface of the second connecting plate, and the other ends of the third springs are fixedly mounted on the lower surface of the inner partition plate.

Preferably, the end part of the second connecting plate far away from the second connecting rod is fixedly provided with a limiting baffle, one side of the bottom of the limiting baffle is provided with an end limiting block, the limiting baffle is arranged in an installation limiting groove, and the installation limiting groove is arranged in the bearing plate and is positioned on the outer side of the sealing plate membrane main body.

Compared with the prior art, the invention has the following beneficial effects:

1. through the cooperation between first fixture block and the draw-in groove, can make the press bar promote the second connecting rod and remove, and then can make the limit baffle of installation spacing inslot remove downwards, make limit baffle can seal all around shrouding membrane main part and PCR board, through setting up the tip stopper in the limit baffle bottom, can make limit baffle can and carry out better laminating between the shrouding membrane main part, make the sealed effect of shrouding membrane main part better, make the inboard difficult pollution that receives because of external factor of PCR, can make the testing result more accurate.

2. According to the depression bar can drive the first connecting plate of its both sides at the in-process that moves down and remove, can make second fixture block and mounting groove cooperate under the head rod effect, have two spacing splint effects through second spring movable mounting in the mounting groove under, it is spacing to make two spacing splint to the position chucking of second fixture block, can make the laminating effect between shrouding membrane main part and the PCR board better, can not produce great positional deviation at the laminating in-process, more conveniently use.

Drawings

FIG. 1 is a schematic diagram of the overall structure of a strong-sealing PCR sealing plate film according to the present invention;

FIG. 2 is a schematic structural diagram of a sealing plate film main body of a strong-sealing PCR sealing plate film according to the present invention;

FIG. 3 is another schematic view of the sealing plate film main body of the strong-sealing PCR sealing plate film according to the present invention;

FIG. 4 is an enlarged view of the portion A of the strong-sealing PCR sealing plate film of the present invention shown in FIG. 1;

FIG. 5 is an enlarged view of the portion B of the strong-sealing PCR sealing plate film of the present invention shown in FIG. 3;

FIG. 6 is a front view of a strong sealing PCR sealing plate film according to the present invention;

FIG. 7 is a schematic cross-sectional view taken along line C-C of FIG. 6 of a strong sealing PCR sealing plate membrane of the present invention;

FIG. 8 is a schematic cross-sectional view taken along line D-D in FIG. 6 of a strong sealing PCR sealing plate film according to the present invention;

FIG. 9 is a schematic cross-sectional view taken along line E-E in FIG. 6 of a strong sealing PCR sealing plate membrane of the present invention;

FIG. 10 is an enlarged view of the membrane of the present invention at F in FIG. 8.

In the figure: 1. a carrier plate; 2. a sealing plate film main body; 201. a sealing cover; 202. sealing the column; 203. leading in a limiting ring; 3. installing a shell; 4. an adjustment mechanism; 401. a pressing lever; 402. moving the mounting block; 403. installing a sliding block; 404. a limiting chute; 405. an auxiliary push rod; 406. a limiting rod; 407. a limiting hole; 408. a limiting plate; 409. a first clamping block; 410. a first mounting plate; 411. a first spring; 5. an inner partition plate; 6. a first limit mechanism; 601. a first connecting plate; 602. a second mounting plate; 603. a first connecting rod; 604. a second fixture block; 605. a first guide portion; 606. mounting grooves; 607. a second spring; 608. a limiting clamp plate; 609. a second guide portion; 7. a second limiting mechanism; 701. a second connecting rod; 702. a card slot; 703. a second connecting plate; 704. a third spring; 705. a limit baffle; 706. an end limiting block; 707. and installing a limiting groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

As shown in fig. 1-10, a strong-sealing PCR sealing plate film includes a bearing plate 1, a sealing plate film main body 2 is disposed on a lower surface of the bearing plate 1, a plurality of sealing covers 201 are uniformly distributed on the lower surface of the sealing plate film main body 2, a sealing post 202 is disposed on a lower surface of each sealing cover 201, a guiding limit ring 203 is mounted at a bottom of each sealing post 202, an installation housing 3 is fixedly disposed on an upper surface of the bearing plate 1, an adjusting mechanism 4 is disposed at a center of an upper surface of the installation housing 3, an inner partition 5 is disposed inside the installation housing 3, a first limit mechanism 6 is disposed inside the installation housing 3 and above the inner partition 5, and a second limit mechanism 7 is disposed inside the installation housing 3 and below the inner partition 5.

In this embodiment, the adjusting mechanism 4 includes a pressing rod 401 and a movable mounting block 402, the pressing rod 401 is movably disposed at the center of the upper surface of the mounting housing 3, a mounting slider 403 is fixedly mounted on the lower surface of the movable mounting block 402, the mounting slider 403 is slidably disposed in a limiting sliding groove 404, an auxiliary push rod 405 is disposed on the two end surfaces of the movable mounting block 402, a limiting rod 406 is fixedly mounted on one side surface of the movable mounting block 402 close to the pressing rod 401, and the movable mounting block 402 is slidably mounted through the mounting slider 403 slidably disposed in the limiting sliding groove 404, so that the limiting rod 406 is more convenient to adjust.

It should be noted that, evenly distributed is provided with a plurality of spacing hole 407 on pressing rod 401, the upper surface of installation casing 3 and the one side that is located to keep away from removal installation piece 402 are provided with limiting plate 408, press rod 401 to be located the inside one end fixed surface of installation casing 3 and install first fixture block 409, through setting up limiting plate 408, can pass spacing hole 407 when spacing rod 406 carries on spacingly to the position of spacing rod 406, make the difficult emergence of position of spacing rod 406 change, and then can make the fixed effect of pressing rod 401 better.

When specifically setting up, the press rod 401 is located the inside one side fixed surface of installation shell 3 and is installed first mounting panel 410, and the lower surface of first mounting panel 410 is provided with first spring 411, and the other end fixed mounting of first spring 411 is at the upper surface of interior baffle 5, under the elastic action of first spring 411, can accomplish the reset work after pressing the press rod 401, and is more convenient when using.

Wherein, the first limiting mechanism 6 comprises a first connecting plate 601 and a second mounting plate 602, the first connecting plate 601 is fixedly mounted on the two end surfaces of the pressing rod 401 inside the mounting housing 3, the second mounting plate 602 is fixedly mounted on the end surface of the first connecting plate 601 far away from the pressing rod 401, the two sides of the lower surface of the second mounting plate 602 are fixedly mounted with first connecting rods 603, the other end of each first connecting rod 603 penetrates through the inner partition plate 5 and the end part is fixedly mounted with a second clamping block 604, the two sides of the bottom of the second clamping block 604 are provided with first guiding parts 605, the upper surface of the bearing plate 1 is uniformly distributed with four mounting grooves 606, the inner walls of the two ends of each mounting groove 606 are fixedly mounted with second springs 607, the other end of each second spring 607 is fixedly mounted with a limiting clamping plate 608, one side of the top of each limiting clamping plate 608 is provided with a second guiding part 609, the limiting clamping plate 608 is arranged below the second clamping block 604, the position of first connecting plate 601 removes and can drive head rod 603 and remove to can make second fixture block 604 cooperate with mounting groove 606, under two spacing splint 608's that pass through second spring 607 movable mounting's effect, can make two spacing splint 608 spacing to the position chucking of second fixture block 604, make the laminating effect between shrouding membrane main part 2 and the PCR board better, can not produce great positional deviation at the laminating in-process, it is more convenient to use.

It should be noted that the second limiting mechanism 7 includes a second connecting rod 701 and a clamping groove 702, the second connecting rod 701 is movably installed at the center of the inner partition 5, the top surface of the second connecting rod 701 is provided with the clamping groove 702, one end of the second connecting rod 701 below the inner partition 5 is fixedly installed with a second connecting plate 703, the upper surface of the second connecting plate 703 is uniformly distributed with a plurality of third springs 704, the other end of the third springs 704 is fixedly installed at the lower surface of the inner partition 5, the end of the second connecting plate 703 far away from the second connecting rod 701 is fixedly installed with a limiting baffle 705, one side of the bottom of the limiting baffle 705 is provided with an end limiting block 706, the limiting baffle 705 is arranged in an installation limiting groove 707, the installation limiting groove 707 is arranged in the bearing plate 1 and located at the outer side of the sealing plate membrane main body 2, the second connecting rod 701 can be pushed to move by the first clamping block 409 and the clamping groove 702 through the cooperation, and then make limit baffle 705 remove, make limit baffle 705 can seal around shrouding membrane main part 2 and PCR board, end stopper 706 can make limit baffle 705 can and shrouding membrane main part 2 between can carry out better laminating, make its sealing performance better.

The working principle of the PCR seal plate membrane with strong sealing performance is as follows:

when the sealing plate film main body 2 is used, the sealing plate film main body 2 is packaged on a PCR plate, each hole on the PCR plate can be sealed independently by arranging a plurality of sealing columns 202, the size of the guide-in limiting ring 203 is slightly larger than that of the sealing columns 202, the sealing columns 202 can not be easily popped out when external force is received, the sealing cover 201 is arranged at the upper end of each sealing column 202, the sealing effect is better, when the sealing plate film main body 2 is packaged, the first fixture block 409 can be matched with the fixture groove 702 by pressing the pressing rod 401, the pressing rod 401 can push the second connecting rod 701 to be pressed downwards, when the second connecting rod 701 moves downwards, the second connecting plate can move downwards, the limiting baffle arranged in the limiting groove 707 can move downwards, the limiting baffle 705 can seal the peripheries of the sealing plate film main body 2 and the PCR plate 705, and the end limiting stopper 706 arranged at the bottom of the limiting baffle 703, the limit baffle 705 can be better attached to the sealing plate film main body 2, the sealing effect of the sealing plate film main body 2 is better, the inner part of the PCR plate is not easy to be polluted by external factors, the detection result is more accurate, the pressing rod 401 can drive the first connecting plates 601 at two sides of the pressing rod to move in the downward moving process, so that the first connecting rod 603 arranged at the lower surface of the second mounting plate 602 can move, the second fixture block 604 at the bottom of the first connecting rod 603 can be matched with the mounting groove 606, two limit clamp plates 608 are movably arranged in the mounting groove 606 through a second spring 607, the two limit clamp plates 608 can clamp and limit the position of the second fixture block 604 under the elastic action of the spring, the attaching effect between the sealing plate film main body 2 and the PCR plate is better, larger position deviation cannot be generated in the attaching process, and the use is more convenient, after pressing down to the assigned position according to pressure pole 401, promote through auxiliary push rod 405 and remove installation piece 402 and remove to make gag lever post 406 pass and press down appointed spacing hole 407 on the pole 401, and then can carry out spacing fixed to the position according to pressure pole 401, thereby can make first stop gear 6 and second stop gear 7 inject more firmly to the position of shrouding membrane main part 2, make the sealed effect of shrouding membrane main part 2 better.

The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a strong leakproofness PCR envelope membrane, includes loading board (1), its characterized in that: the lower surface of loading board (1) is provided with a sealing board membrane main body (2), the lower surface evenly distributed of sealing board membrane main body (2) is provided with a plurality of sealed lid (201), every the lower surface of sealed lid (201) all is provided with sealed post (202), every leading-in spacing ring (203) are all installed to the bottom of sealed post (202), the last fixed surface of loading board (1) is provided with installation casing (3), the upper surface center department of installation casing (3) is provided with adjustment mechanism (4), the internally mounted of installation casing (3) has interior baffle (5), the inside of installation casing (3) and the top that is located interior baffle (5) are provided with first stop gear (6), the inside of installation casing (3) and the below that is located interior baffle (5) are provided with second stop gear (7).

2. A strong sealing PCR sealing plate film according to claim 1, wherein: the adjusting mechanism (4) comprises a pressing rod (401) and a movable mounting block (402), the pressing rod (401) is movably arranged at the center of the upper surface of the mounting shell (3), and a mounting sliding block (403) is fixedly mounted on the lower surface of the movable mounting block (402).

3. A strong sealing PCR sealing plate film according to claim 2, wherein: the mounting sliding block (403) is arranged in the limiting sliding groove (404) in a sliding mode, the surfaces of two ends of the movable mounting block (402) are provided with auxiliary push rods (405), and the surface of one side, close to the pressing rod (401), of the movable mounting block (402) is fixedly provided with a limiting rod (406).

4. A strong sealing PCR sealing plate film according to claim 3, wherein: according to last evenly distributed of pressure lever (401) is provided with a plurality of spacing hole (407), the upper surface of installation casing (3) and be located the one side of keeping away from removal installation piece (402) are provided with limiting plate (408), press lever (401) to be located the inside one end fixed surface of installation casing (3) and install first fixture block (409).

5. A strong sealing PCR sealing plate film according to claim 4, wherein: the pressing rod (401) is located one side surface fixed mounting of installation casing (3) inside and is had first mounting panel (410), the lower surface of first mounting panel (410) is provided with first spring (411), the other end fixed mounting of first spring (411) is at the upper surface of interior baffle (5).

6. A strong sealing PCR sealing plate film according to claim 2, wherein: first stop gear (6) are including first connecting plate (601) and second mounting panel (602), first connecting plate (601) fixed mounting is located the inside both ends surface of installation casing (3) according to pressure bar (401), second mounting panel (602) fixed mounting is kept away from the one end surface of pressing bar (401) at first connecting plate (601).

7. A strong sealing PCR sealing plate film according to claim 6, wherein: first connecting rods (603) are fixedly mounted on two sides of the lower surface of the second mounting plate (602), the other end of each first connecting rod (603) penetrates through the inner partition plate (5), a second clamping block (604) is fixedly mounted at the end of each first connecting rod, and first guide portions (605) are arranged on two sides of the bottom of each second clamping block (604).

8. A strong sealing PCR sealing plate film according to claim 7, wherein: the upper surface evenly distributed of loading board (1) is provided with four mounting grooves (606), every the both ends inner wall fixed mounting of mounting groove (606) has second spring (607), every the other end of second spring (607) all fixed mounting have spacing splint (608), every top one side of spacing splint (608) all is provided with second guide part (609), spacing splint (608) set up the below at second fixture block (604).

9. A strong sealing PCR sealing plate film according to claim 1, wherein: the second limiting mechanism (7) comprises a second connecting rod (701) and a clamping groove (702), the second connecting rod (701) is movably mounted at the center of the inner partition plate (5), the clamping groove (702) is formed in the top surface of the second connecting rod (701), a second connecting plate (703) is fixedly mounted at one end of the second connecting rod (701) located below the inner partition plate (5), a plurality of third springs (704) are uniformly distributed on the upper surface of the second connecting plate (703), and the other ends of the third springs (704) are fixedly mounted on the lower surface of the inner partition plate (5).

10. A strong sealing PCR sealing plate film according to claim 9, wherein: the end part of the second connecting plate (703), which is far away from the second connecting rod (701), is fixedly provided with a limiting baffle (705), one side of the bottom of the limiting baffle (705) is provided with an end limiting block (706), the limiting baffle (705) is arranged in an installation limiting groove (707), and the installation limiting groove (707) is arranged in the bearing plate (1) and is positioned on the outer side of the sealing plate membrane main body (2).