CN114276285A

CN114276285A - Carotenoid preparation, and preparation method and application thereof

Info

Publication number: CN114276285A
Application number: CN202111633948.7A
Authority: CN
Inventors: 李倩; 耿佳鑫; 李晓贺; 陈剑彬; 范超; 吴文忠
Original assignee: Innobio Corp ltd
Current assignee: Innobio Corp ltd
Priority date: 2021-12-28
Filing date: 2021-12-28
Publication date: 2022-04-05

Abstract

The invention discloses a carotenoid preparation, a preparation method and application thereof, wherein the preparation is prepared by mixing pretreated carotenoid and pretreated gelation wall material, and obtaining a powder or granule product through emulsification treatment and granulation; the dosage of the gelation wall material is 60-80% of the final product weight of the carotenoid preparation; the raw material of the pretreated gelation wall material comprises wall material and carbohydrate which are mixed according to the weight ratio of 1: 1-5; the wall material is selected from at least two of starch, Arabic gum and cellulose derivatives; the product produced by the method has a pigment dissolution rate of less than 1% in water, and can effectively maintain biological activity. Can be widely applied to the fields of food, beverage, health care products, medicine and the like, and particularly has good application prospect in the fields of preparing soft sweets, solid beverages, liquid beverages and the like which require the visual effect of nutrients.

Description

Carotenoid preparation, and preparation method and application thereof

Technical Field

The invention relates to the technical field of particle preparation, and particularly relates to a carotenoid preparation, and a preparation method and application thereof.

Background

Carotenoids (carotenoids) are yellow, orange-red or red polyenes, generally composed of 8 isoprenoid units, of the formula C₄₀H₅₆. Carotenoids are known to have beneficial effects on health, and are physiological antioxidants that block lipid peroxidation and thus protect the follicular and uterine steroidogenic cells from oxidation. The carotenoid is provitamin A, and has effects of preventing nyctalopia, resisting oxidation, preventing cancer, and improving tinting strength. However, carotenoids are insoluble in water, have low solubility in fats and oils, and are unstable to light, oxygen, and heat, thereby limiting their applications. Conventional methods improve the bioavailability and also the coloring power of encapsulated products formed by microencapsulation of carotenoids, for example, some beautiful cakes and beverages are derived from carotenoids.

Meanwhile, the improvement of the coloring capability brings troubles, for example, the product is easily dyed on hands, clothes and even faces, and after the carotenoid product is taken, the surface of the tongue and a cup are also dyed, so that a bad experience is brought to people. None of the methods can maintain the biological activity of the carotenoid, reduce the dyeing ability of the carotenoid, and the like, so that the carotenoid can be better used by people, and is a problem which is considered by researchers in the field. A common solution to this problem is to crosslink the gelatin or protein to give crosslinked beads.

For example, patent GB993138 discloses a method for stabilizing gelatin-containing vitamin products, in which formaldehyde, glutaraldehyde, or the like is used as a crosslinking agent, and heat treatment is performed.

Patent CN1283454A, provides a method for preparing beads of fat-soluble substances by crosslinking gelatin using radiation or glutaminase, followed by spray drying into starchy powder.

Patent CN1764439A, provides a method for preparing crosslinked beads, which are crosslinked at 90-140 ℃ for 30 seconds-30 minutes.

The main problem of these patents is that high temperatures are used for crosslinking and the crosslinking time is too long, thus destroying the fat-soluble ingredients or carotenoids which are not stable.

Patent CN1303670A provides a powdered stable vitamin and/or carotenoid product and a preparation method thereof, which uses an alkali metal phosphate cross-linking agent to cross-link protein materials such as gelatin, casein and the like, and achieves the effect of insolubilization in water at 100 ℃ within 3 min. Although it is claimed to lower the thermal crosslinking temperature, the actual preparation process still uses the condition that the heating temperature is 55-180 ℃.

The above patent solves or partially solves the problem of water solubility of carotenoids, but uses gelatin and protein, which are obviously crosslinked to partially achieve the purpose of reducing pigment dissolution. However, the prior art does not describe how to prepare products of carotenoid preparations using other types of wall materials.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a carotenoid preparation prepared by taking non-gelatin and protein raw materials as wall materials, a preparation method and application thereof, which can effectively reduce the coloring capability of the carotenoid and simultaneously still maintain the biological activity of the carotenoid application.

The invention provides a carotenoid preparation, mixing pretreated carotenoid and pretreated gelation wall materials, and obtaining a powder or granule product through emulsification treatment and granulation; wherein:

the dosage of the gelation wall material is 60-80% of the final product weight of the carotenoid preparation;

the raw material of the pretreated gelation wall material comprises wall material and carbohydrate which are mixed according to the weight ratio of 1: 1-5;

the wall material is selected from at least two of starch, Arabic gum and cellulose derivatives;

the carbohydrate is selected from sucrose, glucose syrup, xylose, malto-oligosaccharide, fructo-oligosaccharide, solid corn syrup or mixture thereof.

With respect to the above-mentioned technical solutions, preferably, the carotenoid is selected from lutein and its fatty acid ester, zeaxanthin, lycopene, alpha-carotene, beta-carotene, canthaxanthin, astaxanthin or mixtures thereof.

For the above technical solution, preferably, the wall material is a combination of starch and cellulose derivative, and the carbohydrate is sucrose.

For the above technical solution, preferably, the carotenoid is pretreated to have a total pigment content of more than 70%.

For the technical solution mentioned above, preferably, the carotenoid in the preparation is mixed by the following raw materials: the lutein or the fatty acid ester thereof, the zeaxanthin and the beta carotene are mixed according to the weight ratio of (1-3) to (1-3).

For the above technical solution, preferably, the weight ratio of the wall material and the carbohydrate is 1: 1-3.

For the technical scheme, the wall material is preferably a mixture of starch and a cellulose derivative according to the weight ratio of 1: 1-2.

For the above technical solution, preferably, the cellulose derivative is selected from one or a mixture of several of hypromellose, methylcellulose, ethylcellulose and sodium carboxymethylcellulose, and most preferably, hypromellose.

For the above technical solution, preferably, the cellulose derivative has a viscosity of 2-15 cP.

For the above technical solution, preferably, the step of pre-treating the carotenoid further comprises an antioxidant, and the amount of the antioxidant is 5-30% of the weight of the carotenoid.

For the above technical solution, preferably, the antioxidant is selected from ascorbic acid, ascorbyl palmitate, sucrose fatty acid ester, tocopherol, fatty acid ascorbate, butyl hydroxy toluene, butyl hydroxy anisole, propyl gallic acid, tert-butyl hydroxy quinoline or a mixture thereof.

For the technical scheme, preferably, the antioxidant is ascorbic acid, ascorbyl palmitate and sucrose fatty acid ester according to the weight ratio of 2-5: 0.1-3: 0.1-3 mixing.

For the above technical solution, preferably, the zeaxanthin contains (3R,3 'R) -zeaxanthin and (3R, 3' S) -zeaxanthin in a weight ratio of 5% to 15%: 95 to 85 percent.

For the above technical solution, preferably, the dissolution rate of the pigment in water of the carotenoid preparation is less than 1%, and in a more preferred embodiment, the dissolution rate of the pigment in water of the carotenoid preparation can reach below 0.4%.

In a second aspect of the present invention, there is provided a process for preparing the carotenoid preparation, wherein the pretreated carotenoid is prepared by: mixing carotenoid and 3-5 times of 50-70% ethanol water solution, stirring at 40-50 deg.C for at least 20min, high-speed shearing for dispersing, adding antioxidant, and removing solvent at 70-80 deg.C until ethanol residue is less than 10ppm, and water content of crystal is 10-30%;

the preparation method of the pretreated gelatinized wall material comprises the following steps: mixing wall material and carbohydrate at a weight ratio of 1:1-5, making into 50-70% solid content water solution, stirring at 50-70 deg.C for dispersing, and stirring at 80-90 deg.C for 15-45 min.

The third aspect of the invention provides the application of the carotenoid preparation, including the application in the fields of food, beverage, health care products, medicines and the like.

For the above applications, preferably, the carotenoid preparation has a good application prospect in preparing products requiring a nutrient visualization effect, especially in preparing soft sweets, solid beverages and liquid beverages; and application in preparing eye gel and eye drop.

Compared with the prior art, the invention has the following beneficial effects:

1. after the product is treated by the method, the product has the characteristic of reducing pigment dissolution, the pigment dissolution rate of the product is below 1%, and the pigment dissolution rate can reach below 0.4% in a better embodiment, so the method is particularly suitable for being applied to products requiring a nutrient visualization effect.

2. After the carotenoid is pretreated, the stability is improved, and the biological activity of the carotenoid can be still maintained.

3. Organic solvent is not used in the operation process, and the preparation process is green and environment-friendly.

4. The non-gelatin and protein raw materials are used as wall materials, and the method for preparing the carotenoid preparation is enriched.

Drawings

FIG. 1 is a graph of the products prepared by the methods of example 2 and comparative example 6 according to the present invention applied to fondant, showing that a is the appearance of transparent fondant not impregnated with carotenoid prepared by the method of the present invention and B is the product B of zeaxanthin prepared by the method of comparative example 6, which dyes fondant red and transparency of fondant is decreased.

Detailed Description

The present invention is further illustrated by the following examples, but it should be understood that the scope of the present invention is not limited by the examples.

In the present invention, percentages and percentages are by mass unless otherwise specifically indicated. Unless otherwise specified, the experimental methods used are conventional methods, and the materials, reagents and the like used are commercially available.

The carotenoid preparation can be selectively added with one or more of the following components according to the conventional dosage in the field:

(1) water-soluble ingredients such as, but not limited to, glucose, lactose, malto-oligosaccharide, polyethylene glycol, sodium carboxymethylcellulose, and solid glucose syrup.

(2) Surfactants, to solve the problem of the water-insoluble fat or waxy material that the product usually floats on the water surface, increase the water dispersibility of the product. The surfactant can be exemplified by, but not limited to, tween 60, tween 80 or sucrose fatty acid ester.

(3) Binders, such as but not limited to povidone, glycerin, propylene glycol, polyglycerin fatty acid esters, soluble soybean polysaccharides, sodium carboxymethylcellulose.

(4) Suspending agents, such as but not limited to guar gum, xanthan gum, sodium alginate, hydroxypropyl methylcellulose, gellan gum, carrageenan, may be mentioned.

(5) Diluents, such as but not limited to starch, maltodextrin, calcium hydrogen phosphate.

(6) Examples of stabilizers include, but are not limited to, sodium lactate, sodium citrate, magnesium carbonate, sodium bicarbonate, microcrystalline cellulose.

(7) Glidants such as, but not limited to, silicon dioxide, corn starch, calcium silicate may be mentioned.

(8) Antioxidants, such as but not limited to vitamin E, vitamin C and derivatives thereof, may be mentioned.

(9) Examples of the acidity regulator include, but are not limited to, citric acid, lactic acid, and malic acid.

The following methods were used in the present invention to measure and evaluate the products.

The method for measuring the dissolution rate of the pigment comprises the following steps: and adding 50mL of water into 1g of the product, stirring and dissolving for 30min at 90 ℃ and 100 rpm, filtering and transferring into a volumetric flask, washing with 30mL of water once, combining filtrates, and determining the OD value of the maximum absorption wavelength after constant volume. The dissolution rate of the pigment is (OD value/product quality) multiplied by 100%.

The product accelerated stability evaluation method provided by the invention is a method provided by Chinese pharmacopoeia: the pigment content at different times is measured under the conditions of 40 ℃ and 75% RH to determine the stability, and the product stability is expressed by the pigment retention rate. Pigment retention is the ratio of product content to initial content at different times, expressed as a percentage.

The invention relates to the RELEASE degree measurement, which refers to USP (711) DISSOLUTIO, adopts Apparatus 2, rotates at 50rpm, carries out RELEASE degree experiment according to DELYED-RELEASE DOSAGE FORMS method B, selects 0.1N hydrochloric acid solution as RELEASE medium, and measures the RELEASE degree at different time.

EXAMPLE 1 Effect of the method of treating the crystals on the stability of the crystals

(1) 160g of zeaxanthin crystals and 4 times of 60% ethanol aqueous solution are mixed, stirred at 45 ℃ for 30min, then subjected to high-speed shearing dispersion at 10000 revolutions, added with 28g of ascorbic acid, 10g of ascorbyl palmitate and 10g of sucrose fatty acid ester, subjected to solvent removal at 75 ℃, and subjected to ethanol dissolution residue of 8ppm, wherein the water content of the crystals is 12.3%, and the zeaxanthin crystals A are obtained.

(2) 160g of zeaxanthin crystals and 4 times of 60% ethanol aqueous solution are mixed, stirred at 45 ℃ for 30min, then subjected to high-speed shearing dispersion at 10000 revolutions, and added with 28g of ascorbic acid and 10g of ascorbyl palmitate, the solvent is removed at 75 ℃, the ethanol residue is 55ppm, the water content of the crystals is 25%, and zeaxanthin crystals B are obtained.

(3) 160g of zeaxanthin crystals and 8 times of 80% ethanol aqueous solution are mixed, stirred at 45 ℃ for 30min, then subjected to high-speed shearing dispersion at 10000 revolutions, and then 28g of ascorbic acid, 10g of ascorbyl palmitate and 10g of sucrose fatty acid ester are added, solvent is removed at 75 ℃, ethanol residue is 155ppm, and the water content of the crystals is 32%, so that zeaxanthin crystals C are obtained.

(4) The zeaxanthin crystal D is obtained by mixing 160g of zeaxanthin crystals, 28g of ascorbic acid, 10g of ascorbyl palmitate and 10g of sucrose fatty acid ester.

(5) The zeaxanthin crystals a-D were heated at 60 ℃ and the pigment retention was measured at different times, with the results as given in table 1 below:

TABLE 1 pigment Retention at different times

In addition, zeaxanthin with different isomer ratios was selected for comparative experiments, i.e.: the zeaxanthin contains two isomers of (3R,3 'R) -zeaxanthin and (3R, 3' S) -zeaxanthin, and the weight ratio of the two isomers to the zeaxanthin is more than 80%; and the weight ratio between the two isomers (3R,3 'R) -zeaxanthin and (3R, 3' S) -zeaxanthin is 5-15%: when the content is in the range of 95-85%, the result of the step (1) is not affected.

Example 2

178g of lutein crystals and 3 times of 60% ethanol aqueous solution are mixed, stirred for 30min at 45 ℃, then sheared and dispersed at high speed of 10000 r, 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester are added, solvent is removed at 75 ℃, ethanol residue is 7ppm, the water content of the crystals is 10%, and the crystals are frozen for standby at-20 ℃. Preparing water solution with solid content of 50% from 160g of corn starch, 480g of sucrose and 160g of hydroxypropyl methylcellulose (viscosity of 15cP), stirring and dispersing at 60 ℃, heating to 90 ℃, uniformly stirring for 45min, and standing at normal temperature. Mixing the gelatinized wall material and the treated xanthophyll crystal, stirring, emulsifying, and spray drying. The lutein product A is obtained, the dissolution rate of the pigment is 0.2%, and the 8-hour release rate is 3%.

Example 3

212g of beta carrot crystals and 5 times of 50% ethanol aqueous solution are mixed, stirred for 40min at 40 ℃, then subjected to high-speed shearing dispersion at 10000 r, 17g of ascorbic acid, 25.5g of ascorbyl palmitate and 25.5g of sucrose fatty acid ester are added, the solvent is removed at 70 ℃, the ethanol dissolution residue is 5ppm, the water content of the crystals is 15%, and the crystals are frozen for later use at-20 ℃. Preparing 160g of cassava starch, 480g of sucrose and 160g of hydroxypropyl methyl cellulose (the viscosity is 2.5cP) into an aqueous solution with the solid content of 50%, stirring and dispersing at 50 ℃, heating to 80 ℃, uniformly stirring for 15min, and standing at normal temperature. Mixing the gelatinized wall material and the treated xanthophyll crystal, stirring, emulsifying, and spray drying. The beta carrot crystal product A is obtained, the dissolution rate of the pigment is 0.4%, and the 8-hour release rate is 5.2%.

Example 4

Mixing 81g of beta carrot crystal, 162g of lutein ester crystal, 81g of zeaxanthin crystal and 4 times of 70% ethanol aqueous solution, stirring at 50 ℃ for 55min, performing high-speed shearing dispersion at 10000 revolutions, adding 73g of ascorbic acid, 1.5g of ascorbyl palmitate and 1.5g of sucrose fatty acid ester, removing the solvent at 80 ℃, removing 3ppm of ethanol residue, ensuring that the water content of the crystal is 30%, and freezing at-20 ℃ for later use. 100g of potato starch, 300g of sucrose and 200g of hydroxypropyl methyl cellulose (viscosity 5cP) are prepared into an aqueous solution with solid content of 50%, the aqueous solution is stirred and dispersed at 70 ℃, the temperature is raised to 82 ℃, the uniform stirring is carried out for 30min, and the aqueous solution is placed at the normal temperature. Mixing the gelatinized wall material and the treated xanthophyll crystal, stirring, emulsifying, and spray drying. The lutein ester, zeaxanthin and beta carrot product A with the ratio of 2:1:1 are obtained, the pigment dissolution rate is 0.26 percent, and the 8-hour release rate is 6.1 percent.

Comparative example 5

Mixing 200g of lutein crystal with 3 times of 60% ethanol aqueous solution, stirring at 45 ℃ for 30min, performing high-speed shearing dispersion at 10000 revolutions, adding 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, removing solvent at 75 ℃, dissolving ethanol residue by 7ppm, ensuring that the water content of the crystal is 10%, and freezing at-20 ℃ for later use. 583g of sodium octenyl succinate starch and 195g of glucose are prepared into an aqueous solution with solid content of 50 percent, and after stirring and dispersing at 60 ℃, the temperature is raised to 90 ℃, and stirring is carried out at constant speed for 35 min. Cooling to 60 deg.C, and stirring for 100 min. Mixing the gelatinized wall material and the treated xanthophyll crystal, stirring, emulsifying, and spray drying. The lutein product A is obtained, the dissolution rate of the pigment is 3.1 percent, and the 8-hour release rate is 101.6 percent. The release rates at different times are shown in table 2 below:

TABLE 2 Release degree at different times

Time	Degree of release	Time	Degree of release
				10min	10.0％	3h	101.6％
20min	16.0％	4h	103.3％
				30min	30.5％	6h	101.6％
1h	40.2％	8h	101.6％
				2h	89.4％

According to the technical scheme of the comparative example 5, through the combination of the octenyl sodium starch succinate and the glucose, the release degree of the obtained product is superior to that of the technical schemes of the examples 1 to 4, but the dissolution rate is poor, and according to the analysis of effect data, the pigment dissolution rate of the product obtained according to the technical scheme of the comparative example 5 is 3.1% which is 8-15 times of the pigment dissolution rate (0.2-0.4%) of the products obtained in the examples 1 to 4, so that the technical scheme of the application is far superior to that of the prior art in the aspect of improving the pigment dissolution rate of the product, and has extremely remarkable effect improvement.

Comparative example 6

178g of zeaxanthin crystals and 3 times of 60% ethanol aqueous solution are mixed, stirred at 45 ℃ for 30min, then subjected to high-speed shearing dispersion at 10000 revolutions, added with 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, subjected to solvent removal at 75 ℃, 7ppm of ethanol residues and 12.8% of water content of the crystals, and frozen at-20 ℃ for later use. Preparing 160g of cassava starch, 480g of sucrose and 160g of hydroxypropyl methyl cellulose (viscosity is 10cP) into an aqueous solution with solid content of 50%, stirring and dissolving, adding the zeaxanthin crystals, stirring, emulsifying, and spray-drying. The pigment dissolution rate of the zeaxanthin product B is 67.7 percent. The 8h release degree is 99.1%.

The contrast shows that the chroma of the product is improved and the slow release effect is deteriorated by the wall material which is not subjected to gelation treatment.

Comparative example 7

Adding 200g of lutein crystal into 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, mixing, preparing a 50% solid content aqueous solution from 160g of cassava starch, 480g of sucrose and 160g of hydroxypropyl methyl cellulose (viscosity is 10cP), stirring and dispersing at 50 ℃, heating to 80 ℃, uniformly stirring for 15min, and standing at normal temperature. Mixing the gelatinized wall material and the lutein crystal, stirring, emulsifying, and spray drying. The lutein product B with the pigment dissolution rate of 30.2 percent is obtained. The 8h release degree is 95.8%.

As can be seen by comparison, the chroma and the release degree of the product are both deteriorated without the pretreatment of the crystals.

Comparative example 8

200g of lutein ester crystals and 3 times of 60% ethanol aqueous solution are mixed, stirred for 30min at 45 ℃, then subjected to high-speed shearing dispersion at 10000 revolutions, added with 20g of ascorbic acid, 1g of ascorbyl palmitate and 1g of sucrose fatty acid ester, subjected to solvent removal at 75 ℃, and subjected to ethanol residue removal of 7ppm, wherein the water content of the crystals is 10%, and then frozen at-20 ℃ for later use. The gelling wall material compositions in the table are respectively added, and the product effect is shown in the following table 3 after the treatment according to the gelling method in the example 2:

TABLE 3 comparison of dissolution effects using different gelling wall materials

In contrast, the combination of a wall material (corn starch or hydroxypropylmethyl cellulose) and a carbohydrate alone is not effective. And the formula does not contain carbohydrate, so the effect is poor. The effect of the formula in which corn starch and Arabic gum are used as wall materials and are gelatinized together with sucrose is also poor. The experimental group using hydroxypropylmethylcellulose having a high viscosity as a wall material also did not achieve the expected effect.

Effect example 9

Lutein, zeaxanthin and beta-carotene were prepared according to the method of patent 201711456450.1 at a weight ratio of 1:1:1 to obtain composite product 1, and lutein, zeaxanthin and beta-carotene were prepared using the method of example 2 at a weight ratio of 1:1:1 to obtain composite product 2, the comparative parameters for the two products are as follows in table 4:

TABLE 4 two product comparison parameters

	Bulk density	6 months retention accelerated at 40 ℃	Dissolution rate of pigment	Degree of release of 8h
					Composite product 1	0.68	98.7％	68.2％	102.3
Composite product 2	0.66	98.9％	0.32％	5.3％

Effect example 10: evaluation of fondant applications

Weighing 8g of gelatin, 44g of white granulated sugar and 55g of glucose syrup, adding water with 20% of solid matters, stirring for dissolving, decocting at 120 ℃ until the solid matters are about 85%, and adjusting the pH value to 3-4; solution I was obtained.

The lutein product A obtained by the method of example 2 or the zeaxanthin product B20g obtained by the method of example 6 are added into the solution I and stirred uniformly. Keeping the temperature at 90 ℃ for 40 min. And (5) injection molding and drying. The appearance is shown in picture 1. It can be seen that the lutein product A has no staining phenomenon in the soft sweets, is completely stored in the soft sweets, and realizes the effect of nutrition visualization. The zeaxanthin product B stained the fondant red and the transparency of the fondant was reduced.

Effect example 11

0.03 part of the lutein product A prepared by the method in the embodiment 2 and 0.02 part of the lutein ester product B prepared by the method in the embodiment 8 are respectively mixed with proper amount of taurine, 0.002 part of vitamin A, 0.3 part of hyaluronic acid, 12 parts of glycerol, 9 parts of sodium chloride, 0.2 part of methyl paraben, 0.1 part of menthol and proper amount of water for injection, and the lutein eye drops are obtained by filtering, filling and sterilizing. The effect of the eye drops was evaluated as shown in table 5:

table 5: evaluation of application Effect of eye drops

Solid beverage numbering	Eye drop color	Eye drops particle size
			Xanthophyll product A	Transparent and colorless	250nm
Lutein ester product B	Light yellow	800nm

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and those skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims

1. A carotenoid preparation is characterized in that a powder or granule product is obtained by mixing pretreated carotenoid and pretreated gelation wall material, emulsifying and granulating; wherein:

the carbohydrate is at least one selected from sucrose, glucose syrup, xylose, malto-oligosaccharide, fructo-oligosaccharide and solid corn syrup.

2. The carotenoid formulation according to claim 1, wherein the carotenoid is at least one selected from the group consisting of lutein and fatty acid esters thereof, zeaxanthin, lycopene, alpha-carotene, beta-carotene, canthaxanthin, and astaxanthin.

3. The carotenoid formulation of claim 1 wherein the carotenoid has a total pigment content of greater than 70% after pretreatment.

4. The carotenoid formulation according to claim 1, wherein the carotenoid is mixed from the following raw materials: the lutein or the fatty acid ester thereof, the zeaxanthin and the beta carotene are mixed according to the weight ratio of (1-3) to (1-3).

5. The carotenoid preparation according to claim 1, wherein the wall material is a mixture of starch and cellulose derivative in a weight ratio of 1: 1-2.

6. The carotenoid formulation of claim 1 wherein the cellulose derivative is selected from at least one of hypromellose, methylcellulose, ethylcellulose, and sodium carboxymethylcellulose.

7. The carotenoid formulation according to claim 6, characterized in that the viscosity of the cellulose derivative is 2-15 cP.

8. The carotenoid formulation of claim 1 wherein the step of pre-treating the carotenoid further comprises an antioxidant in an amount of 5-30% by weight of the carotenoid.

9. The carotenoid formulation according to claim 8, wherein the antioxidant is at least one selected from ascorbic acid, ascorbyl palmitate, sucrose fatty acid ester, tocopherol, fatty acid ascorbate, butyl hydroxytoluene, butyl hydroxyanisole, propyl gallic acid, and tert-butyl hydroxyquinoline.

10. The carotenoid formulation according to claim 9, wherein the antioxidant is ascorbic acid, ascorbyl palmitate or sucrose fatty acid ester in a weight ratio of 2-5: 0.1-3: 0.1-3 mixing.

11. The carotenoid formulation according to claim 1, wherein the pigment dissolution in water is less than 1%.

12. The method of producing a carotenoid formulation according to claim 1, wherein the pretreated carotenoid is produced by: mixing carotenoid and 3-5 times of 50-70% ethanol water solution, stirring at 40-50 deg.C for at least 20min, high-speed shearing for dispersing, adding antioxidant, and removing solvent at 70-80 deg.C until ethanol residue is less than 10ppm, and water content of crystal is 10-30%;

13. Use of the carotenoid preparation according to claim 1 in the fields of food, beverage, health product and medicine.

14. Use according to claim 13, characterized in that: the carotenoid preparation is applied to the preparation of products requiring the visual effect of nutrients, soft sweets, solid beverages and liquid beverages, or the preparation of eye gels and eye drops.