CN114196677A - 一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala - Google Patents

Abstract

本发明涉及一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala。本发明的突变体没有破坏或抑制FⅧ的凝血活性，凝血比活性为野生型的1.5～2倍，在稳定性上表现更优越；突变体明显提高了FⅧ的药物效能，具备很好的临床应用前景。

Description

一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala

技术领域

本发明属于血友病领域，特别涉及一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala。

背景技术

血友病A是由于凝血因子Ⅷ(FⅧ)缺陷所致遗传性出血性疾病，男性人群中发病率为1/5000，患者凝血功能障碍，表现为自发的肌肉血肿和关节腔出血等。FⅧ替代治疗是目前唯一有效的治疗手段。随着生物技术的发展，基因重组的FⅧ，逐渐取代了血浆或者血浆来源的FⅧ成为替代治疗的主要制剂。FⅧ体内半衰期仅有不到12小时，为了维持足够的血浆浓度以预防出血，每周需要注射3次，费用昂贵。开发具有更高凝血活性的FⅧ制剂，既可以用较少的剂量达到相同的治疗效果，又能减少抑制物产生的机率，将会为临床治疗血友病A提供很大的帮助。

发明内容

本发明所要解决的技术问题是提供一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala，该突变体没有破坏或抑制FⅧ的凝血活性，凝血比活性为野生型的1.5～2倍，且在稳定性上表现更优越；突变体明显提高了FⅧ的药物效能，具备很好的临床应用前景。

本发明提供了一种编码高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的核酸：

(1)hFⅧcDNA：核苷酸序列如SEQ ID NO:1-4任一所示；

或(2)BDD hFⅧ-SQ：核苷酸序列如SEQ ID NO:5-8任一所示；

或(3)BDD hFⅧ-N8：核苷酸序列如SEQ ID NO:9-12任一所示；

或(4)BDD hFⅧ-SC：核苷酸序列如SEQ ID NO:13-16任一所示；

或(5)BDD hFⅧ-△F：核苷酸序列如SEQ ID NO:17-20任一所示。

本发明还提供了一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白，氨基酸序列如SEQ ID NO:21-29任一所示，该突变***于第710位的氨基酸为Ala而非野生型Ⅷ或Ⅷa的Gly。所述氨基酸序列依次包括hFⅧcDNA、BDD hFⅧ-SQ、BDD hFⅧ-N8、BDD hFⅧ-SC、BDD hFⅧ-△F对应的突变蛋白的氨基酸序列以及BDD hFⅧ-V3、BDD human-cl rhFⅧ、pFⅧ、BDD pFⅧ-OL的突变蛋白的氨基酸序列。

本发明还提供了编码所述的突变蛋白的核酸，或与所述编码核酸长度相同且与所述编码核酸完全互补的核酸。

本发明还提供了一种表达所述的突变蛋白的载体。

本发明还提供了一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白的制备方法，包括如下步骤：

以BDD-hFⅧ或全长FⅧ为模板，用PCR定点诱变法在野生型FⅧ中引入突变位点，将Gly710氨基酸残基替换成Ala，经转化后筛选出正确***Gly710Ala突变的BDD-hFⅧ或全长FⅧ真核表达质粒。

本发明还提供了一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白编码核酸的应用，应用所述的核酸于制备基因治疗药物，包括将其与启动子和终止序列连接，构建表达质粒、基因治疗病毒或非病毒载体，表达突变蛋白。

本发明还提供了一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白的应用，应用于制备血友病的重组蛋白治疗药物。

本发明还提供了一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白的应用，应用于制备凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala突变体融合蛋白，并将所述融合蛋白用于制备血友病的重组蛋白治疗药物。

所述融合蛋白为由人白蛋白、免疫球蛋白Fc、转铁蛋白或alpha 1抗胰蛋白酶中的一种与所述的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala突变体的突变蛋白融合而得。

本发明的核酸或氨基酸序列的药物组合物或基因治疗载体，用于预防和/或治疗疾病，其中所述疾病主要包括出血性疾病或各种原因导致的出血；其中，最可能的出血性疾病是血友病A和B，即由于遗传性凝血因子Ⅷ缺乏导致的出血性疾病，并包括其中有抑制性抗体产生的血友病A和B，或者后天因抑制物产生所导致的获得性凝血因子Ⅷ缺乏；及其它使用旁路制剂的出血性疾病，例如新生儿凝血障碍；严重的肝脏疾病；高风险手术；创伤性失血；骨髓移植；血小板减少症和血小板功能障碍；口服抗凝的紧急逆转；先天性凝血因子Ⅷ的缺陷；血管性血友病，及血管性血友病因子抑制物导致的获得性血管性血友病，与大量损伤有关的失血，大脑出血，血小板功能障碍。

有益效果

本发明的突变体没有破坏或抑制FⅧ的凝血活性，凝血比活性为野生型的1.5～2倍，且在稳定性上表现更优越；突变体明显提高了FⅧ的药物效能，具备很好的临床应用前景。

附图说明

图1为无FⅨa存在下BDD-hFⅧGly710Ala突变体的FⅧ活性时程检测。

图2为FⅨa存在下BDD-hFⅧGly710Ala突变体的FⅧ活性时程检测。

图3为BDD-hFⅧGly710Ala突变体热稳定性检测。

具体实施方式

下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。此外应理解，在阅读了本发明讲授的内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。

实施例1

高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白，氨基酸序列如SEQIDNO:21-29任一所示，该突变***于第710位的氨基酸为Ala而非野生型Ⅷ或Ⅷa的Gly。本实施例以hFⅧcDNA的突变蛋白为例。

1.FⅧGly710Ala突变体的表达和纯化

(1)表达：利用QuikChange定点突变试剂盒(

Ⅱ XL Site-DirectedMutagenesis Kit,Agilent,美国)，以人B区缺失型凝血Ⅷ因子(BDD-hFⅧ)为模板，用PCR定点诱变法在野生型FⅧ中引入突变位点，将710Gly替换成710Ala,扩增产物经过Dpn I消化后进行转化(每100微升感受态细菌中加入5-10微升经过Dpn I消化后的突变产物)，被转化后的细菌涂布到含有1/2000氨苄青霉素的平板上培养过夜得到克隆；挑取单个菌落，接种到5ml含有1/2000氨苄青霉素的LB培养液，250转/min、37℃摇床上过夜扩菌，提取质粒DNA进行测序，筛选出正确***Gly710Ala突变的BDD-hFⅧ真核表达质粒。

(2)纯化：分别用野生型BDD-hFⅧ、构建好的BDD-hFⅧGly710Ala表达质粒转染CHO细胞，并设立空白载体对照。筛选出稳定高表达克隆细胞株后，转入选择性无血清培养液中进行大规模培养，用免疫吸附法(免疫吸附凝胶Ⅷ-Select，GE Healthcare，美国)将分泌至培养液中的FⅧ进行蛋白纯化。用Centricon Plus超滤离心管(MILLIPORE，美国)对产物进行浓缩。

2.FⅧ Gly710Ala突变体的凝血活性检测

(1)FⅧGly710Ala突变体的辅酶活性检测(FXa生成试验)：FⅧ的辅酶活性体现为辅助FIXa裂解FX成为FXa的能力。以野生型BDD-hFⅧ作为对照，应用FXa生成实验对FⅧGly710Ala突变体的辅酶活性进行评估：①凝血酶作用于FⅧ1min(FⅧ:凝血酶＝20:1)，使FⅧ活化成为FⅧa，水蛭素灭活凝血酶；②取FⅧa 0.5nM，加PSPC磷脂颗粒20μM、FIXa40nM，混合后37℃孵育1min，分别加入0nM，20nM，40nM，60nM，120nM，240nM等不同浓度的FX，37℃反应10min，EDTA终止反应；③加入FXa特异性发色底物S-2765，405nm波长下比色，测定所产生的FXa。应用graphpad prism软件进行Michaelis-Menten酶动力学分析，计算酶动力常数Km、Kcat和Vmax。

(2)FⅧGly710Ala突变体对FⅧ比活性的影响：FⅧ的比活性代表的是每毫克蛋白的FⅧ所具有的凝血活性。使用一期法和二期法分别测定纯化好的BDD-hFⅧ突变体和野生型的活性，二期法(Chromogenicassay)按照

SP4FⅧ试剂盒(Chromogenix，意大利)流程操作。用酶联免疫吸附测定法进行蛋白定量：采用抗-C2抗体GMA-8014作为捕获抗体，生物素标记的抗A2抗体GMA-8016作为检测抗体，纯化的重组人FⅧ作为标准，采用三明治式ELISA确定样品的浓度。计算出FⅧ突变体的比活性以及FⅧ突变体相对于野生型的相对比活性。

3.FⅧ Gly710Ala突变体对FⅧ稳定性的影响

(1)FⅧ热稳定试验：以野生型FⅧ作为对照，观察FⅧGly710Ala突变体在高温条件下活性丢失的程度，评估其稳定性。取野生型FⅧ及FⅧ突变体各4nM，55℃孵育，分别取样于孵育后0min、10min、20min、30min、40min，应用发色底物法检测残留的FⅧ活性,经graphpadprism软件分析得出one phase decay模型拟合衰减曲线，得出半衰期。

(2)FⅧa活性衰减实验：

①无FⅨa存在下的FⅧ活性时程：分别取FⅧGly710Ala及野生型BDD-hFⅧ各4nM，经凝血酶20nM活化后水蛭素灭活凝血酶,加入FIXa 40nM、PSPC 10μM，混合后23℃孵育，分别取样于孵育后0min、5min、10min、15min、20min，加入FX，比照FXa生成试验程序检测样本FXa浓度，间接确定残留FⅧa的浓度。

②FⅨa存在下的FⅧ活性时程：重复上述步骤，在添加凝血酶之前，在反应中添加因子IXa40nM。经凝血酶20nM活化后水蛭素灭活凝血酶,加入PSPC 10μM，混合后23℃孵育，分别取样于孵育后0min、10min、20min、30min、40min，加入FX，比照FXa生成试验程序检测样本FXa浓度，间接确定残留FⅧa的浓度。

③以上两个实验结果用graphpad prism的one phase decay模型拟合出衰减曲线，得出半衰期，比较FⅧa的稳定性。

4.实验结果

(1)BDD-hFⅧ Gly710Ala突变体相对比活性检测：

质粒瞬时转染CHO细胞，培养48h后收集培养液，取上清液进行检测，用APTT一期法检测突变体的活性，用ELISA法检测突变体的蛋白量，与野生型BDD-hFⅧ比较，初步得出突变体的相对比活性。结果显示：在8次实验中，BDD-hFⅧGly710Ala突变体的相对比活性为1.597±0.402。

筛选BDD-hFⅧGly710Ala突变体稳定表达的细胞株进行大容量细胞培养，分别于48h、72h收集培养液，低温环境下提取、纯化、浓缩后得到目的蛋白。分别用一期法、二期法检测突变体的活性，用ELISA法检测突变体的蛋白量，与野生型BDD-hFⅧ比较，计算突变体的相对比活性。结果显示：在6次实验中，BDD-hFⅧGly710Ala突变体在一期法的相对比活性为2.214±0.767，在二期法的相对比活性为1.521±0.068。

(2)BDD-hFⅧGly710Ala突变体辅酶活性与稳定性的检测：

采用上述大容量细胞培养产物进行检测，通过FXa生成试验、FⅧ活性时程试验等检测突变体的辅酶活性与稳定性，结果显示，在相同的活性浓度下，突变体不但具有与野生型相当的辅酶性能和稳定性，在FIXa存在下表现出更好的稳定性。

①通过FXa生成试验检测FⅧGly710Ala突变体的辅酶作用。结果显示：FⅧGly710位点突变为Ala后，仍然表现出良好的辅酶性能。

②通过无Ⅸa存在下的FⅧ活性时程检测FⅧGly710Ala突变体的辅酶状态与稳定性。如图1结果显示：在不含FIXa的反应中，23℃条件下FⅧGly710Ala突变体与野生型BDD-hFⅧ活性衰减50％的时间接近，提示两者具有相似的辅酶状态、A2亚单位的保留率以及稳定性。

③通过检测FⅨa存在下的FⅧ活性时程观察FⅧ突变体对FⅧ稳定性的影响。如图2结果显示：在含有FIXa的反应中，23℃条件下FⅧGly710Ala突变体活性衰减50％的时间约为野生型的1.5倍。提示突变体在结合酶FⅨa的作用下，突变体稳定性高于野生型。

④BDD-hFⅧGly710Ala突变体热稳定性检测：如图3结果显示，在55℃条件反应下，FⅧGly710Ala突变体与野生型BDD活性衰减50％的时间相当，提示两者在高温下具有相似的热稳定性。

序列表

<110> 广西医科大学第一附属医院

<120> 一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala

<130> 1

<160> 29

<170> SIPOSequenceListing 1.0

<210> 1

<211> 7056

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 1

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggct tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400

atacaaaatg tctcctctag tgatttgttg atgctcttgc gacagagtcc tactccacat 2460

gggctatcct tatctgatct ccaagaagcc aaatatgaga ctttttctga tgatccatca 2520

cctggagcaa tagacagtaa taacagcctg tctgaaatga cacacttcag gccacagctc 2580

catcacagtg gggacatggt atttacccct gagtcaggcc tccaattaag attaaatgag 2640

aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700

tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760

agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820

tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880

aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940

aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000

gctttgttga ctaaagataa tgccttattc aaagttagca tctctttgtt aaagacaaac 3060

aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120

attgagaata gtccatcagt ctggcaaaat atattagaaa gtgacactga gtttaaaaaa 3180

gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240

aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300

gagggcccca ttccaccaga tgcacaaaat ccagatatgt cgttctttaa gatgctattc 3360

ttgccagaat cagcaaggtg gatacaaagg actcatggaa agaactctct gaactctggg 3420

caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480

aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540

ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600

ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660

gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720

aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780

ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840

aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900

aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960

agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020

ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080

aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140

aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200

caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260

atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320

agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380

aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440

ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500

aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560

cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620

gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680

ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740

ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800

tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860

aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920

atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980

gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040

attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100

gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160

gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220

gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280

tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340

tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400

ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460

cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520

catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580

gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640

acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700

atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760

ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820

aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880

tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940

gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000

gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060

attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120

cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180

caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240

tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300

cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360

atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420

accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480

cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540

cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600

agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660

acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720

caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780

ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840

atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900

gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960

ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020

gaggttctgg gctgcgaggc acaggacctc tactag 7056

<210> 2

<211> 7056

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 2

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggcc tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400

atacaaaatg tctcctctag tgatttgttg atgctcttgc gacagagtcc tactccacat 2460

gggctatcct tatctgatct ccaagaagcc aaatatgaga ctttttctga tgatccatca 2520

cctggagcaa tagacagtaa taacagcctg tctgaaatga cacacttcag gccacagctc 2580

catcacagtg gggacatggt atttacccct gagtcaggcc tccaattaag attaaatgag 2640

aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700

tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760

agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820

tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880

aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940

aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000

gctttgttga ctaaagataa tgccttattc aaagttagca tctctttgtt aaagacaaac 3060

aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120

attgagaata gtccatcagt ctggcaaaat atattagaaa gtgacactga gtttaaaaaa 3180

gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240

aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300

gagggcccca ttccaccaga tgcacaaaat ccagatatgt cgttctttaa gatgctattc 3360

ttgccagaat cagcaaggtg gatacaaagg actcatggaa agaactctct gaactctggg 3420

caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480

aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540

ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600

ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660

gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720

aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780

ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840

aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900

aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960

agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020

ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080

aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140

aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200

caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260

atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320

agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380

aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440

ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500

aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560

cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620

gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680

ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740

ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800

tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860

aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920

atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980

gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040

attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100

gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160

gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220

gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280

tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340

tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400

ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460

cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520

catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580

gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640

acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700

atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760

ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820

aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880

tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940

gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000

gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060

attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120

cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180

caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240

tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300

cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360

atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420

accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480

cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540

cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600

agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660

acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720

caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780

ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840

atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900

gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960

ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020

gaggttctgg gctgcgaggc acaggacctc tactag 7056

<210> 3

<211> 7056

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 3

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggca tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400

atacaaaatg tctcctctag tgatttgttg atgctcttgc gacagagtcc tactccacat 2460

gggctatcct tatctgatct ccaagaagcc aaatatgaga ctttttctga tgatccatca 2520

cctggagcaa tagacagtaa taacagcctg tctgaaatga cacacttcag gccacagctc 2580

catcacagtg gggacatggt atttacccct gagtcaggcc tccaattaag attaaatgag 2640

aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700

tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760

agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820

tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880

aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940

aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000

gctttgttga ctaaagataa tgccttattc aaagttagca tctctttgtt aaagacaaac 3060

aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120

attgagaata gtccatcagt ctggcaaaat atattagaaa gtgacactga gtttaaaaaa 3180

gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240

aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300

gagggcccca ttccaccaga tgcacaaaat ccagatatgt cgttctttaa gatgctattc 3360

ttgccagaat cagcaaggtg gatacaaagg actcatggaa agaactctct gaactctggg 3420

caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480

aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540

ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600

ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660

gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720

aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780

ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840

aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900

aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960

agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020

ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080

aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140

aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200

caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260

atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320

agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380

aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440

ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500

aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560

cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620

gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680

ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740

ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800

tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860

aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920

atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980

gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040

attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100

gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160

gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220

gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280

tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340

tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400

ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460

cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520

catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580

gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640

acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700

atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760

ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820

aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880

tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940

gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000

gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060

attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120

cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180

caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240

tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300

cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360

atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420

accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480

cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540

cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600

agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660

acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720

caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780

ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840

atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900

gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960

ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020

gaggttctgg gctgcgaggc acaggacctc tactag 7056

<210> 4

<211> 7056

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 4

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggcg tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaatg acatagagaa gactgaccct tggtttgcac acagaacacc tatgcctaaa 2400

atacaaaatg tctcctctag tgatttgttg atgctcttgc gacagagtcc tactccacat 2460

gggctatcct tatctgatct ccaagaagcc aaatatgaga ctttttctga tgatccatca 2520

cctggagcaa tagacagtaa taacagcctg tctgaaatga cacacttcag gccacagctc 2580

catcacagtg gggacatggt atttacccct gagtcaggcc tccaattaag attaaatgag 2640

aaactgggga caactgcagc aacagagttg aagaaacttg atttcaaagt ttctagtaca 2700

tcaaataatc tgatttcaac aattccatca gacaatttgg cagcaggtac tgataataca 2760

agttccttag gacccccaag tatgccagtt cattatgata gtcaattaga taccactcta 2820

tttggcaaaa agtcatctcc ccttactgag tctggtggac ctctgagctt gagtgaagaa 2880

aataatgatt caaagttgtt agaatcaggt ttaatgaata gccaagaaag ttcatgggga 2940

aaaaatgtat cgtcaacaga gagtggtagg ttatttaaag ggaaaagagc tcatggacct 3000

gctttgttga ctaaagataa tgccttattc aaagttagca tctctttgtt aaagacaaac 3060

aaaacttcca ataattcagc aactaataga aagactcaca ttgatggccc atcattatta 3120

attgagaata gtccatcagt ctggcaaaat atattagaaa gtgacactga gtttaaaaaa 3180

gtgacacctt tgattcatga cagaatgctt atggacaaaa atgctacagc tttgaggcta 3240

aatcatatgt caaataaaac tacttcatca aaaaacatgg aaatggtcca acagaaaaaa 3300

gagggcccca ttccaccaga tgcacaaaat ccagatatgt cgttctttaa gatgctattc 3360

ttgccagaat cagcaaggtg gatacaaagg actcatggaa agaactctct gaactctggg 3420

caaggcccca gtccaaagca attagtatcc ttaggaccag aaaaatctgt ggaaggtcag 3480

aatttcttgt ctgagaaaaa caaagtggta gtaggaaagg gtgaatttac aaaggacgta 3540

ggactcaaag agatggtttt tccaagcagc agaaacctat ttcttactaa cttggataat 3600

ttacatgaaa ataatacaca caatcaagaa aaaaaaattc aggaagaaat agaaaagaag 3660

gaaacattaa tccaagagaa tgtagttttg cctcagatac atacagtgac tggcactaag 3720

aatttcatga agaacctttt cttactgagc actaggcaaa atgtagaagg ttcatatgac 3780

ggggcatatg ctccagtact tcaagatttt aggtcattaa atgattcaac aaatagaaca 3840

aagaaacaca cagctcattt ctcaaaaaaa ggggaggaag aaaacttgga aggcttggga 3900

aatcaaacca agcaaattgt agagaaatat gcatgcacca caaggatatc tcctaataca 3960

agccagcaga attttgtcac gcaacgtagt aagagagctt tgaaacaatt cagactccca 4020

ctagaagaaa cagaacttga aaaaaggata attgtggatg acacctcaac ccagtggtcc 4080

aaaaacatga aacatttgac cccgagcacc ctcacacaga tagactacaa tgagaaggag 4140

aaaggggcca ttactcagtc tcccttatca gattgcctta cgaggagtca tagcatccct 4200

caagcaaata gatctccatt acccattgca aaggtatcat catttccatc tattagacct 4260

atatatctga ccagggtcct attccaagac aactcttctc atcttccagc agcatcttat 4320

agaaagaaag attctggggt ccaagaaagc agtcatttct tacaaggagc caaaaaaaat 4380

aacctttctt tagccattct aaccttggag atgactggtg atcaaagaga ggttggctcc 4440

ctggggacaa gtgccacaaa ttcagtcaca tacaagaaag ttgagaacac tgttctcccg 4500

aaaccagact tgcccaaaac atctggcaaa gttgaattgc ttccaaaagt tcacatttat 4560

cagaaggacc tattccctac ggaaactagc aatgggtctc ctggccatct ggatctcgtg 4620

gaagggagcc ttcttcaggg aacagaggga gcgattaagt ggaatgaagc aaacagacct 4680

ggaaaagttc cctttctgag agtagcaaca gaaagctctg caaagactcc ctccaagcta 4740

ttggatcctc ttgcttggga taaccactat ggtactcaga taccaaaaga agagtggaaa 4800

tcccaagaga agtcaccaga aaaaacagct tttaagaaaa aggataccat tttgtccctg 4860

aacgcttgtg aaagcaatca tgcaatagca gcaataaatg agggacaaaa taagcccgaa 4920

atagaagtca cctgggcaaa gcaaggtagg actgaaaggc tgtgctctca aaacccacca 4980

gtcttgaaac gccatcaacg ggaaataact cgtactactc ttcagtcaga tcaagaggaa 5040

attgactatg atgataccat atcagttgaa atgaagaagg aagattttga catttatgat 5100

gaggatgaaa atcagagccc ccgcagcttt caaaagaaaa cacgacacta ttttattgct 5160

gcagtggaga ggctctggga ttatgggatg agtagctccc cacatgttct aagaaacagg 5220

gctcagagtg gcagtgtccc tcagttcaag aaagttgttt tccaggaatt tactgatggc 5280

tcctttactc agcccttata ccgtggagaa ctaaatgaac atttgggact cctggggcca 5340

tatataagag cagaagttga agataatatc atggtaactt tcagaaatca ggcctctcgt 5400

ccctattcct tctattctag ccttatttct tatgaggaag atcagaggca aggagcagaa 5460

cctagaaaaa actttgtcaa gcctaatgaa accaaaactt acttttggaa agtgcaacat 5520

catatggcac ccactaaaga tgagtttgac tgcaaagcct gggcttattt ctctgatgtt 5580

gacctggaaa aagatgtgca ctcaggcctg attggacccc ttctggtctg ccacactaac 5640

acactgaacc ctgctcatgg gagacaagtg acagtacagg aatttgctct gtttttcacc 5700

atctttgatg agaccaaaag ctggtacttc actgaaaata tggaaagaaa ctgcagggct 5760

ccctgcaata tccagatgga agatcccact tttaaagaga attatcgctt ccatgcaatc 5820

aatggctaca taatggatac actacctggc ttagtaatgg ctcaggatca aaggattcga 5880

tggtatctgc tcagcatggg cagcaatgaa aacatccatt ctattcattt cagtggacat 5940

gtgttcactg tacgaaaaaa agaggagtat aaaatggcac tgtacaatct ctatccaggt 6000

gtttttgaga cagtggaaat gttaccatcc aaagctggaa tttggcgggt ggaatgcctt 6060

attggcgagc atctacatgc tgggatgagc acactttttc tggtgtacag caataagtgt 6120

cagactcccc tgggaatggc ttctggacac attagagatt ttcagattac agcttcagga 6180

caatatggac agtgggcccc aaagctggcc agacttcatt attccggatc aatcaatgcc 6240

tggagcacca aggagccctt ttcttggatc aaggtggatc tgttggcacc aatgattatt 6300

cacggcatca agacccaggg tgcccgtcag aagttctcca gcctctacat ctctcagttt 6360

atcatcatgt atagtcttga tgggaagaag tggcagactt atcgaggaaa ttccactgga 6420

accttaatgg tcttctttgg caatgtggat tcatctggga taaaacacaa tatttttaac 6480

cctccaatta ttgctcgata catccgtttg cacccaactc attatagcat tcgcagcact 6540

cttcgcatgg agttgatggg ctgtgattta aatagttgca gcatgccatt gggaatggag 6600

agtaaagcaa tatcagatgc acagattact gcttcatcct actttaccaa tatgtttgcc 6660

acctggtctc cttcaaaagc tcgacttcac ctccaaggga ggagtaatgc ctggagacct 6720

caggtgaata atccaaaaga gtggctgcaa gtggacttcc agaagacaat gaaagtcaca 6780

ggagtaacta ctcagggagt aaaatctctg cttaccagca tgtatgtgaa ggagttcctc 6840

atctccagca gtcaagatgg ccatcagtgg actctctttt ttcagaatgg caaagtaaag 6900

gtttttcagg gaaatcaaga ctccttcaca cctgtggtga actctctaga cccaccgtta 6960

ctgactcgct accttcgaat tcacccccag agttgggtgc accagattgc cctgaggatg 7020

gaggttctgg gctgcgaggc acaggacctc tactag 7056

<210> 6

<211> 4374

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 6

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgcc tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340

cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400

gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460

cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520

cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580

caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640

ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700

cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760

cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820

ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880

gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940

ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000

ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060

gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120

taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180

caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240

atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300

tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360

tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420

gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480

cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540

tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600

ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660

ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720

cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780

aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840

tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900

atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960

tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020

tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080

aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140

tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200

caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260

tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320

cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374

<210> 5

<211> 4374

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 5

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgct tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340

cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400

gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460

cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520

cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580

caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640

ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700

cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760

cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820

ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880

gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940

ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000

ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060

gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120

taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180

caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240

atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300

tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360

tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420

gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480

cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540

tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600

ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660

ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720

cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780

aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840

tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900

atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960

tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020

tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080

aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140

tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200

caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260

tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320

cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374

<210> 7

<211> 4374

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 7

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgca tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340

cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400

gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460

cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520

cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580

caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640

ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700

cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760

cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820

ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880

gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940

ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000

ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060

gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120

taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180

caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240

atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300

tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360

tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420

gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480

cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540

tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600

ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660

ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720

cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780

aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840

tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900

atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960

tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020

tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080

aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140

tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200

caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260

tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320

cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374

<210> 8

<211> 4374

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 8

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgcg tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaagagg catcagcggg agatcacccg cacgaccctg 2340

cagtcggatc aggaggagat tgattacgac gacacgatca gtgtggagat gaagaaggag 2400

gacttcgaca tctacgacga agatgaaaac cagtcccctc ggtccttcca aaagaagacc 2460

cggcactact tcatcgccgc tgtggaacgc ctgtgggact atggaatgtc ttctagccct 2520

cacgttttga ggaaccgcgc ccagtcgggc agcgtgcccc agttcaagaa agtggtgttc 2580

caggagttca ccgacggctc cttcacccag ccactttacc ggggcgagct caatgaacat 2640

ctgggcctgc tgggacccta catcagggct gaggtggagg acaacatcat ggtgacattc 2700

cggaatcagg ccagcagacc atacagtttc tacagttcac tcatctccta cgaggaggac 2760

cagcgccagg gggctgaacc ccgtaagaac ttcgtgaagc caaacgaaac aaagacctac 2820

ttctggaagg tccagcacca catggcacct accaaggacg agttcgattg caaggcctgg 2880

gcctacttct ccgacgtgga cctggagaaa gatgtgcaca gcggcctgat tggccctctg 2940

ctggtgtgtc acacgaacac actcaaccct gcacacgggc ggcaggtcac tgtgcaggaa 3000

ttcgccctgt tctttaccat ctttgatgag acgaagtcct ggtatttcac cgaaaacatg 3060

gagaggaact gccgcgcacc ctgcaacatc cagatggaag atccgacatt caaggagaac 3120

taccggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct cgtgatggcc 3180

caagaccagc gtatccgctg gtatctgctg tcgatgggct ccaacgagaa catccatagt 3240

atccacttca gcgggcatgt cttcacggtg aggaaaaagg aggagtacaa gatggcactg 3300

tacaacctct atcccggcgt gttcgagacc gtggagatgc tgccctccaa ggccggcatc 3360

tggagagtgg aatgcctgat cggcgagcac ctccacgctg ggatgtccac gctgttcctc 3420

gtttacagca ataagtgcca gacccctctg ggcatggcga gcggccacat ccgcgacttc 3480

cagattacag ccagcggcca gtacggtcag tgggctccaa agctggcccg tctgcactac 3540

tccggatcca tcaacgcctg gtccaccaag gaaccgttct cctggatcaa agtagacctg 3600

ctagccccca tgatcattca cggcatcaag acacaaggcg cccgacagaa gttctcgagc 3660

ctctatatct cccagttcat catcatgtat agcctggacg gaaagaagtg gcagacttac 3720

cgcggaaact cgacagggac cctgatggta ttcttcggta acgtggacag ctccggaatc 3780

aagcacaaca tcttcaaccc acccattatc gcccgctaca tccgcctgca ccccactcac 3840

tatagcatta ggtccaccct gcgaatggag ctcatgggct gtgacctgaa cagctgtagc 3900

atgcccctcg gcatggagtc taaggcgatc tccgacgcac agataacggc atcatcctac 3960

tttaccaaca tgttcgctac ctggtccccc tccaaggccc gactccacct gcaagggaga 4020

tccaacgcct ggcggccaca ggtcaacaat cccaaggagt ggctgcaagt ggactttcag 4080

aaaactatga aagtcaccgg agtgaccaca cagggagtga agtctctgct gaccagcatg 4140

tacgtgaagg agttcctcat ctccagttcg caggatggcc accagtggac gttgttcttc 4200

caaaacggta aagtcaaagt cttccaaggg aaccaggaca gctttacacc cgtcgtgaac 4260

tccctggacc ccccgcttct cactagatac ctccgcatcc accctcagag ctgggtgcac 4320

cagattgccc tgcgcatgga ggttctgggg tgtgaagccc aggacctgta ctaa 4374

<210> 9

<211> 4395

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 9

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggct tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340

gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400

tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460

cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520

tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580

cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640

cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700

gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760

cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820

cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880

gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940

tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000

agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060

tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120

gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180

ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240

agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300

gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360

ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420

gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480

tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540

aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600

tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660

gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720

gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780

aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840

atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900

tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960

cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020

cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080

tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140

aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200

catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260

tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320

cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380

caggacctct actag 4395

<210> 10

<211> 4395

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 10

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggcc tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340

gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400

tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460

cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520

tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580

cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640

cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700

gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760

cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820

cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880

gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940

tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000

agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060

tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120

gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180

ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240

agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300

gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360

ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420

gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480

tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540

aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600

tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660

gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720

gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780

aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840

atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900

tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960

cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020

cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080

tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140

aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200

catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260

tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320

cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380

caggacctct actag 4395

<210> 11

<211> 4395

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 11

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggca tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340

gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400

tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460

cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520

tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580

cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640

cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700

gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760

cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820

cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880

gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940

tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000

agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060

tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120

gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180

ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240

agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300

gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360

ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420

gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480

tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540

aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600

tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660

gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720

gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780

aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840

atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900

tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960

cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020

cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080

tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140

aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200

catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260

tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320

cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380

caggacctct actag 4395

<210> 12

<211> 4395

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 12

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggcg tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca cccttctcaa aacccaccag tcttgaaacg ccatcaacgg 2340

gaaataactc gtactactct tcagtcagat caagaggaaa ttgactatga tgataccata 2400

tcagttgaaa tgaagaagga agattttgac atttatgatg aggatgaaaa tcagagcccc 2460

cgcagctttc aaaagaaaac acgacactat tttattgctg cagtggagag gctctgggat 2520

tatgggatga gtagctcccc acatgttcta agaaacaggg ctcagagtgg cagtgtccct 2580

cagttcaaga aagttgtttt ccaggaattt actgatggct cctttactca gcccttatac 2640

cgtggagaac taaatgaaca tttgggactc ctggggccat atataagagc agaagttgaa 2700

gataatatca tggtaacttt cagaaatcag gcctctcgtc cctattcctt ctattctagc 2760

cttatttctt atgaggaaga tcagaggcaa ggagcagaac ctagaaaaaa ctttgtcaag 2820

cctaatgaaa ccaaaactta cttttggaaa gtgcaacatc atatggcacc cactaaagat 2880

gagtttgact gcaaagcctg ggcttatttc tctgatgttg acctggaaaa agatgtgcac 2940

tcaggcctga ttggacccct tctggtctgc cacactaaca cactgaaccc tgctcatggg 3000

agacaagtga cagtacagga atttgctctg tttttcacca tctttgatga gaccaaaagc 3060

tggtacttca ctgaaaatat ggaaagaaac tgcagggctc cctgcaatat ccagatggaa 3120

gatcccactt ttaaagagaa ttatcgcttc catgcaatca atggctacat aatggataca 3180

ctacctggct tagtaatggc tcaggatcaa aggattcgat ggtatctgct cagcatgggc 3240

agcaatgaaa acatccattc tattcatttc agtggacatg tgttcactgt acgaaaaaaa 3300

gaggagtata aaatggcact gtacaatctc tatccaggtg tttttgagac agtggaaatg 3360

ttaccatcca aagctggaat ttggcgggtg gaatgcctta ttggcgagca tctacatgct 3420

gggatgagca cactttttct ggtgtacagc aataagtgtc agactcccct gggaatggct 3480

tctggacaca ttagagattt tcagattaca gcttcaggac aatatggaca gtgggcccca 3540

aagctggcca gacttcatta ttccggatca atcaatgcct ggagcaccaa ggagcccttt 3600

tcttggatca aggtggatct gttggcacca atgattattc acggcatcaa gacccagggt 3660

gcccgtcaga agttctccag cctctacatc tctcagttta tcatcatgta tagtcttgat 3720

gggaagaagt ggcagactta tcgaggaaat tccactggaa ccttaatggt cttctttggc 3780

aatgtggatt catctgggat aaaacacaat atttttaacc ctccaattat tgctcgatac 3840

atccgtttgc acccaactca ttatagcatt cgcagcactc ttcgcatgga gttgatgggc 3900

tgtgatttaa atagttgcag catgccattg ggaatggaga gtaaagcaat atcagatgca 3960

cagattactg cttcatccta ctttaccaat atgtttgcca cctggtctcc ttcaaaagct 4020

cgacttcacc tccaagggag gagtaatgcc tggagacctc aggtgaataa tccaaaagag 4080

tggctgcaag tggacttcca gaagacaatg aaagtcacag gagtaactac tcagggagta 4140

aaatctctgc ttaccagcat gtatgtgaag gagttcctca tctccagcag tcaagatggc 4200

catcagtgga ctctcttttt tcagaatggc aaagtaaagg tttttcaggg aaatcaagac 4260

tccttcacac ctgtggtgaa ctctctagac ccaccgttac tgactcgcta ccttcgaatt 4320

cacccccaga gttgggtgca ccagattgcc ctgaggatgg aggttctggg ctgcgaggca 4380

caggacctct actag 4395

<210> 13

<211> 4392

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 13

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggct tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400

gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460

agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520

gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580

ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640

ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700

aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760

atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820

aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880

tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940

ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000

caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060

tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120

cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180

cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240

aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300

gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360

ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420

atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480

ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540

ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600

tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660

cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720

aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780

gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840

cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900

gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960

attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020

cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080

ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140

tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200

cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260

ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320

ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380

gacctctact ag 4392

<210> 14

<211> 4392

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 14

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggcc tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400

gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460

agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520

gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580

ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640

ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700

aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760

atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820

aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880

tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940

ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000

caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060

tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120

cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180

cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240

aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300

gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360

ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420

atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480

ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540

ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600

tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660

cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720

aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780

gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840

cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900

gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960

attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020

cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080

ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140

tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200

cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260

ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320

ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380

gacctctact ag 4392

<210> 15

<211> 4392

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 15

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggca tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400

gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460

agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520

gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580

ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640

ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700

aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760

atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820

aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880

tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940

ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000

caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060

tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120

cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180

cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240

aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300

gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360

ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420

atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480

ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540

ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600

tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660

cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720

aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780

gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840

cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900

gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960

attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020

cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080

ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140

tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200

cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260

ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320

ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380

gacctctact ag 4392

<210> 16

<211> 4392

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 16

atgcaaatag agctctccac ctgcttcttt ctgtgccttt tgcgattctg ctttagtgcc 60

accagaagat actacctggg tgcagtggaa ctgtcatggg actatatgca aagtgatctc 120

ggtgagctgc ctgtggacgc aagatttcct cctagagtgc caaaatcttt tccattcaac 180

acctcagtcg tgtacaaaaa gactctgttt gtagaattca cggatcacct tttcaacatc 240

gctaagccaa ggccaccctg gatgggtctg ctaggtccta ccatccaggc tgaggtttat 300

gatacagtgg tcattacact taagaacatg gcttcccatc ctgtcagtct tcatgctgtt 360

ggtgtatcct actggaaagc ttctgaggga gctgaatatg atgatcagac cagtcaaagg 420

gagaaagaag atgataaagt cttccctggt ggaagccata catatgtctg gcaggtcctg 480

aaagagaatg gtccaatggc ctctgaccca ctgtgcctta cctactcata tctttctcat 540

gtggacctgg taaaagactt gaattcaggc ctcattggag ccctactagt atgtagagaa 600

gggagtctgg ccaaggaaaa gacacagacc ttgcacaaat ttatactact ttttgctgta 660

tttgatgaag ggaaaagttg gcactcagaa acaaagaact ccttgatgca ggatagggat 720

gctgcatctg ctcgggcctg gcctaaaatg cacacagtca atggttatgt aaacaggtct 780

ctgccaggtc tgattggatg ccacaggaaa tcagtctatt ggcatgtgat tggaatgggc 840

accactcctg aagtgcactc aatattcctc gaaggtcaca catttcttgt gaggaaccat 900

cgccaggcgt ccttggaaat ctcgccaata actttcctta ctgctcaaac actcttgatg 960

gaccttggac agtttctact gttttgtcat atctcttccc accaacatga tggcatggaa 1020

gcttatgtca aagtagacag ctgtccagag gaaccccaac tacgaatgaa aaataatgaa 1080

gaagcggaag actatgatga tgatcttact gattctgaaa tggatgtggt caggtttgat 1140

gatgacaact ctccttcctt tatccaaatt cgctcagttg ccaagaagca tcctaaaact 1200

tgggtacatt acattgctgc tgaagaggag gactgggact atgctccctt agtcctcgcc 1260

cccgatgaca gaagttataa aagtcaatat ttgaacaatg gccctcagcg gattggtagg 1320

aagtacaaaa aagtccgatt tatggcatac acagatgaaa cctttaagac tcgtgaagct 1380

attcagcatg aatcaggaat cttgggacct ttactttatg gggaagttgg agacacactg 1440

ttgattatat ttaagaatca agcaagcaga ccatataaca tctaccctca cggaatcact 1500

gatgtccgtc ctttgtattc aaggagatta ccaaaaggtg taaaacattt gaaggatttt 1560

ccaattctgc caggagaaat attcaaatat aaatggacag tgactgtaga agatgggcca 1620

actaaatcag atcctcggtg cctgacccgc tattactcta gtttcgttaa tatggagaga 1680

gatctagctt caggactcat tggccctctc ctcatctgct acaaagaatc tgtagatcaa 1740

agaggaaacc agataatgtc agacaagagg aatgtcatcc tgttttctgt atttgatgag 1800

aaccgaagct ggtacctcac agagaatata caacgctttc tccccaatcc agctggagtg 1860

cagcttgagg atccagagtt ccaagcctcc aacatcatgc acagcatcaa tggctatgtt 1920

tttgatagtt tgcagttgtc agtttgtttg catgaggtgg catactggta cattctaagc 1980

attggagcac agactgactt cctttctgtc ttcttctctg gatatacctt caaacacaaa 2040

atggtctatg aagacacact caccctattc ccattctcag gagaaactgt cttcatgtcg 2100

atggaaaacc caggtctatg gattctggcg tgccacaact cagactttcg gaacagaggc 2160

atgaccgcct tactgaaggt ttctagttgt gacaagaaca ctggtgatta ttacgaggac 2220

agttatgaag atatttcagc atacttgctg agtaaaaaca atgccattga accaagaagc 2280

ttctcccaga attcaagaca ccctagcact aggcaaaagc aatttaatgc caccacaatt 2340

ccagaaaata ctactcttca gtcagatcaa gaggaaattg actatgatga taccatatca 2400

gttgaaatga agaaggaaga ttttgacatt tatgatgagg atgaaaatca gagcccccgc 2460

agctttcaaa agaaaacacg acactatttt attgctgcag tggagaggct ctgggattat 2520

gggatgagta gctccccaca tgttctaaga aacagggctc agagtggcag tgtccctcag 2580

ttcaagaaag ttgttttcca ggaatttact gatggctcct ttactcagcc cttataccgt 2640

ggagaactaa atgaacattt gggactcctg gggccatata taagagcaga agttgaagat 2700

aatatcatgg taactttcag aaatcaggcc tctcgtccct attccttcta ttctagcctt 2760

atttcttatg aggaagatca gaggcaagga gcagaaccta gaaaaaactt tgtcaagcct 2820

aatgaaacca aaacttactt ttggaaagtg caacatcata tggcacccac taaagatgag 2880

tttgactgca aagcctgggc ttatttctct gatgttgacc tggaaaaaga tgtgcactca 2940

ggcctgattg gaccccttct ggtctgccac actaacacac tgaaccctgc tcatgggaga 3000

caagtgacag tacaggaatt tgctctgttt ttcaccatct ttgatgagac caaaagctgg 3060

tacttcactg aaaatatgga aagaaactgc agggctccct gcaatatcca gatggaagat 3120

cccactttta aagagaatta tcgcttccat gcaatcaatg gctacataat ggatacacta 3180

cctggcttag taatggctca ggatcaaagg attcgatggt atctgctcag catgggcagc 3240

aatgaaaaca tccattctat tcatttcagt ggacatgtgt tcactgtacg aaaaaaagag 3300

gagtataaaa tggcactgta caatctctat ccaggtgttt ttgagacagt ggaaatgtta 3360

ccatccaaag ctggaatttg gcgggtggaa tgccttattg gcgagcatct acatgctggg 3420

atgagcacac tttttctggt gtacagcaat aagtgtcaga ctcccctggg aatggcttct 3480

ggacacatta gagattttca gattacagct tcaggacaat atggacagtg ggccccaaag 3540

ctggccagac ttcattattc cggatcaatc aatgcctgga gcaccaagga gcccttttct 3600

tggatcaagg tggatctgtt ggcaccaatg attattcacg gcatcaagac ccagggtgcc 3660

cgtcagaagt tctccagcct ctacatctct cagtttatca tcatgtatag tcttgatggg 3720

aagaagtggc agacttatcg aggaaattcc actggaacct taatggtctt ctttggcaat 3780

gtggattcat ctgggataaa acacaatatt tttaaccctc caattattgc tcgatacatc 3840

cgtttgcacc caactcatta tagcattcgc agcactcttc gcatggagtt gatgggctgt 3900

gatttaaata gttgcagcat gccattggga atggagagta aagcaatatc agatgcacag 3960

attactgctt catcctactt taccaatatg tttgccacct ggtctccttc aaaagctcga 4020

cttcacctcc aagggaggag taatgcctgg agacctcagg tgaataatcc aaaagagtgg 4080

ctgcaagtgg acttccagaa gacaatgaaa gtcacaggag taactactca gggagtaaaa 4140

tctctgctta ccagcatgta tgtgaaggag ttcctcatct ccagcagtca agatggccat 4200

cagtggactc tcttttttca gaatggcaaa gtaaaggttt ttcagggaaa tcaagactcc 4260

ttcacacctg tggtgaactc tctagaccca ccgttactga ctcgctacct tcgaattcac 4320

ccccagagtt gggtgcacca gattgccctg aggatggagg ttctgggctg cgaggcacag 4380

gacctctact ag 4392

<210> 17

<211> 4362

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 17

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgct tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340

gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400

tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460

atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520

aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580

gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640

ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700

agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760

gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820

cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880

gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940

acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000

tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060

cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120

gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180

atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240

gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300

cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360

tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420

aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480

agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540

aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600

atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660

cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720

acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780

ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840

tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900

atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960

ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020

cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080

gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140

ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200

gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260

ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320

cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362

<210> 18

<211> 4362

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 18

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgcc tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340

gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400

tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460

atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520

aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580

gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640

ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700

agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760

gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820

cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880

gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940

acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000

tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060

cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120

gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180

atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240

gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300

cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360

tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420

aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480

agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540

aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600

atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660

cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720

acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780

ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840

tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900

atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960

ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020

cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080

gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140

ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200

gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260

ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320

cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362

<210> 19

<211> 4362

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 19

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgca tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340

gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400

tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460

atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520

aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580

gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640

ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700

agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760

gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820

cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880

gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940

acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000

tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060

cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120

gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180

atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240

gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300

cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360

tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420

aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480

agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540

aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600

atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660

cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720

acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780

ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840

tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900

atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960

ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020

cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080

gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140

ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200

gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260

ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320

cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362

<210> 20

<211> 4362

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 20

atgcagatcg aactgagcac ttgcttcttc ctgtgtctcc tgcgcttttg cttctccgcc 60

acaaggagat actatctcgg tgccgtggag ctcagctggg actacatgca gagcgacttg 120

ggtgaactgc ctgtggacgc caggtttcca ccccgcgtgc ccaagagttt cccgttcaac 180

accagtgtcg tgtacaagaa aaccctcttc gtggaattca ccgaccacct gttcaacatc 240

gccaaaccgc gccctccctg gatggggctg ctcggcccga cgatccaggc tgaggtctat 300

gacacggtgg tgattaccct caagaacatg gctagccacc cggtgagcct gcacgccgtg 360

ggcgtgtcct attggaaagc gtccgagggt gcggagtacg atgaccagac ttcacagcgg 420

gagaaggaag acgacaaagt gttccccggg ggttcccaca cctatgtctg gcaggtcctg 480

aaggagaatg gtcctatggc ctccgaccca ttgtgcctca cctactctta cctaagccat 540

gtggatctcg tcaaggacct gaactcgggg ctgatcggcg ccctgctcgt gtgccgggag 600

ggctcactgg ccaaggagaa gacccaaact ctgcacaagt tcatcctgct gttcgcggta 660

ttcgacgagg ggaagtcctg gcactccgag accaagaaca gcctgatgca ggaccgcgac 720

gcagcctcgg cccgtgcgtg gccaaagatg cacaccgtga acggctacgt taacaggagc 780

ctacccggcc tgatcggctg ccaccgcaaa tcggtctact ggcatgtgat cggaatgggc 840

acaacgcccg aggtccacag tatcttcctc gagggccaca ctttcctggt ccggaatcac 900

cgccaggcca gcctggagat cagccccata acctttctga cggcgcagac cttactcatg 960

gatctcggcc agttcctcct gttctgccac atttcgtccc accagcacga tgggatggaa 1020

gcatatgtga aagtggactc ctgccccgag gaaccccagc ttaggatgaa gaacaatgag 1080

gaggccgagg actacgacga tgaccttacc gattcagaaa tggacgtagt acgctttgac 1140

gacgacaact ctccatcctt catacagatt cgctccgtcg ccaagaagca ccctaagact 1200

tgggtgcact acatcgcggc cgaggaggag gactgggatt atgctcccct ggtgctggcc 1260

cccgacgacc gcagctacaa gagccagtac ctgaataacg ggccccagcg catcggccgg 1320

aagtacaaga aagtgcggtt catggcttac acggacgaga ccttcaagac ccgggaggct 1380

atccagcatg agagcggcat cttggggccc ctcctgtacg gcgaagttgg agacacactg 1440

ctgatcatct tcaagaacca ggcgagcagg ccctacaaca tctaccccca cggcattacc 1500

gatgtccggc cgttgtacag ccgacggctg cccaagggcg tgaagcacct gaaggacttt 1560

ccgatcctgc cgggcgagat cttcaagtac aagtggactg tgaccgtgga ggatgggccg 1620

accaagagcg atccgcgctg cctgacccgt tactactcca gctttgtcaa tatggagcgc 1680

gacctcgcta gcggcttgat tggccctctg ctgatctgct acaaggagtc cgtggaccag 1740

agggggaatc agatcatgag tgacaagagg aacgtgatcc tgttctccgt gttcgacgaa 1800

aaccgcagct ggtatctcac cgagaatatc cagcgcttcc tgcccaaccc ggccggtgtg 1860

cagctggagg accccgagtt tcaggccagc aacatcatgc attctatcaa cggatatgtg 1920

tttgattccc tgcagctctc agtgtgtctg cacgaggtcg cctactggta tatcctcagc 1980

attggggcac agaccgactt cctgagcgtg ttcttctccg ggtatacctt caagcacaag 2040

atggtgtacg aggataccct gaccctgttc ccctttagcg gcgaaaccgt gtttatgtct 2100

atggagaacc ccgggctctg gatccttgcg tgccataact ccgacttccg caaccgcgga 2160

atgaccgcgc tcctgaaagt gtcgagttgt gacaagaaca ccggcgacta ttacgaggac 2220

agttacgagg acatctctgc gtacctcctt agcaagaata acgccatcga gccaagatcc 2280

ttcagccaga accccccagt gctgaaggag atcacccgca cgaccctgca gtcggatcag 2340

gaggagattg attacgacga cacgatcagt gtggagatga agaaggagga cttcgacatc 2400

tacgacgaag atgaaaacca gtcccctcgg tccttccaaa agaagacccg gcactacttc 2460

atcgccgctg tggaacgcct gtgggactat ggaatgtctt ctagccctca cgttttgagg 2520

aaccgcgccc agtcgggcag cgtgccccag ttcaagaaag tggtgttcca ggagttcacc 2580

gacggctcct tcacccagcc actttaccgg ggcgagctca atgaacatct gggcctgctg 2640

ggaccctaca tcagggctga ggtggaggac aacatcatgg tgacattccg gaatcaggcc 2700

agcagaccat acagtttcta cagttcactc atctcctacg aggaggacca gcgccagggg 2760

gctgaacccc gtaagaactt cgtgaagcca aacgaaacaa agacctactt ctggaaggtc 2820

cagcaccaca tggcacctac caaggacgag ttcgattgca aggcctgggc ctacttctcc 2880

gacgtggacc tggagaaaga tgtgcacagc ggcctgattg gccctctgct ggtgtgtcac 2940

acgaacacac tcaaccctgc acacgggcgg caggtcactg tgcaggaatt cgccctgttc 3000

tttaccatct ttgatgagac gaagtcctgg tatttcaccg aaaacatgga gaggaactgc 3060

cgcgcaccct gcaacatcca gatggaagat ccgacattca aggagaacta ccggttccat 3120

gccatcaatg gctacatcat ggacaccctg cctggcctcg tgatggccca agaccagcgt 3180

atccgctggt atctgctgtc gatgggctcc aacgagaaca tccatagtat ccacttcagc 3240

gggcatgtct tcacggtgag gaaaaaggag gagtacaaga tggcactgta caacctctat 3300

cccggcgtgt tcgagaccgt ggagatgctg ccctccaagg ccggcatctg gagagtggaa 3360

tgcctgatcg gcgagcacct ccacgctggg atgtccacgc tgttcctcgt ttacagcaat 3420

aagtgccaga cccctctggg catggcgagc ggccacatcc gcgacttcca gattacagcc 3480

agcggccagt acggtcagtg ggctccaaag ctggcccgtc tgcactactc cggatccatc 3540

aacgcctggt ccaccaagga accgttctcc tggatcaaag tagacctgct agcccccatg 3600

atcattcacg gcatcaagac acaaggcgcc cgacagaagt tctcgagcct ctatatctcc 3660

cagttcatca tcatgtatag cctggacgga aagaagtggc agacttaccg cggaaactcg 3720

acagggaccc tgatggtatt cttcggtaac gtggacagct ccggaatcaa gcacaacatc 3780

ttcaacccac ccattatcgc ccgctacatc cgcctgcacc ccactcacta tagcattagg 3840

tccaccctgc gaatggagct catgggctgt gacctgaaca gctgtagcat gcccctcggc 3900

atggagtcta aggcgatctc cgacgcacag ataacggcat catcctactt taccaacatg 3960

ttcgctacct ggtccccctc caaggcccga ctccacctgc aagggagatc caacgcctgg 4020

cggccacagg tcaacaatcc caaggagtgg ctgcaagtgg actttcagaa aactatgaaa 4080

gtcaccggag tgaccacaca gggagtgaag tctctgctga ccagcatgta cgtgaaggag 4140

ttcctcatct ccagttcgca ggatggccac cagtggacgt tgttcttcca aaacggtaaa 4200

gtcaaagtct tccaagggaa ccaggacagc tttacacccg tcgtgaactc cctggacccc 4260

ccgcttctca ctagatacct ccgcatccac cctcagagct gggtgcacca gattgccctg 4320

cgcatggagg ttctggggtg tgaagcccag gacctgtact aa 4362

<210> 21

<211> 2351

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 21

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe

1 5 10 15

Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg

35 40 45

Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val

50 55 60

Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile

65 70 75 80

Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln

85 90 95

Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser

100 105 110

His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser

115 120 125

Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp

130 135 140

Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu

145 150 155 160

Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser

165 170 175

Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile

180 185 190

Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr

195 200 205

Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly

210 215 220

Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp

225 230 235 240

Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr

245 250 255

Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val

260 265 270

Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile

275 280 285

Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser

290 295 300

Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met

305 310 315 320

Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His

325 330 335

Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro

340 345 350

Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp

355 360 365

Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser

370 375 380

Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn

420 425 430

Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met

435 440 445

Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys

500 505 510

Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp

725 730 735

Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys

740 745 750

Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Pro

755 760 765

Ser Thr Arg Gln Lys Gln Phe Asn Ala Thr Thr Ile Pro Glu Asn Asp

770 775 780

Ile Glu Lys Thr Asp Pro Trp Phe Ala His Arg Thr Pro Met Pro Lys

785 790 795 800

Ile Gln Asn Val Ser Ser Ser Asp Leu Leu Met Leu Leu Arg Gln Ser

805 810 815

Pro Thr Pro His Gly Leu Ser Leu Ser Asp Leu Gln Glu Ala Lys Tyr

820 825 830

Glu Thr Phe Ser Asp Asp Pro Ser Pro Gly Ala Ile Asp Ser Asn Asn

835 840 845

Ser Leu Ser Glu Met Thr His Phe Arg Pro Gln Leu His His Ser Gly

850 855 860

Asp Met Val Phe Thr Pro Glu Ser Gly Leu Gln Leu Arg Leu Asn Glu

865 870 875 880

Lys Leu Gly Thr Thr Ala Ala Thr Glu Leu Lys Lys Leu Asp Phe Lys

885 890 895

Val Ser Ser Thr Ser Asn Asn Leu Ile Ser Thr Ile Pro Ser Asp Asn

900 905 910

Leu Ala Ala Gly Thr Asp Asn Thr Ser Ser Leu Gly Pro Pro Ser Met

915 920 925

Pro Val His Tyr Asp Ser Gln Leu Asp Thr Thr Leu Phe Gly Lys Lys

930 935 940

Ser Ser Pro Leu Thr Glu Ser Gly Gly Pro Leu Ser Leu Ser Glu Glu

945 950 955 960

Asn Asn Asp Ser Lys Leu Leu Glu Ser Gly Leu Met Asn Ser Gln Glu

965 970 975

Ser Ser Trp Gly Lys Asn Val Ser Ser Thr Glu Ser Gly Arg Leu Phe

980 985 990

Lys Gly Lys Arg Ala His Gly Pro Ala Leu Leu Thr Lys Asp Asn Ala

995 1000 1005

Leu Phe Lys Val Ser Ile Ser Leu Leu Lys Thr Asn Lys Thr Ser Asn

1010 1015 1020

Asn Ser Ala Thr Asn Arg Lys Thr His Ile Asp Gly Pro Ser Leu Leu

1025 1030 1035 1040

Ile Glu Asn Ser Pro Ser Val Trp Gln Asn Ile Leu Glu Ser Asp Thr

1045 1050 1055

Glu Phe Lys Lys Val Thr Pro Leu Ile His Asp Arg Met Leu Met Asp

1060 1065 1070

Lys Asn Ala Thr Ala Leu Arg Leu Asn His Met Ser Asn Lys Thr Thr

1075 1080 1085

Ser Ser Lys Asn Met Glu Met Val Gln Gln Lys Lys Glu Gly Pro Ile

1090 1095 1100

Pro Pro Asp Ala Gln Asn Pro Asp Met Ser Phe Phe Lys Met Leu Phe

1105 1110 1115 1120

Leu Pro Glu Ser Ala Arg Trp Ile Gln Arg Thr His Gly Lys Asn Ser

1125 1130 1135

Leu Asn Ser Gly Gln Gly Pro Ser Pro Lys Gln Leu Val Ser Leu Gly

1140 1145 1150

Pro Glu Lys Ser Val Glu Gly Gln Asn Phe Leu Ser Glu Lys Asn Lys

1155 1160 1165

Val Val Val Gly Lys Gly Glu Phe Thr Lys Asp Val Gly Leu Lys Glu

1170 1175 1180

Met Val Phe Pro Ser Ser Arg Asn Leu Phe Leu Thr Asn Leu Asp Asn

1185 1190 1195 1200

Leu His Glu Asn Asn Thr His Asn Gln Glu Lys Lys Ile Gln Glu Glu

1205 1210 1215

Ile Glu Lys Lys Glu Thr Leu Ile Gln Glu Asn Val Val Leu Pro Gln

1220 1225 1230

Ile His Thr Val Thr Gly Thr Lys Asn Phe Met Lys Asn Leu Phe Leu

1235 1240 1245

Leu Ser Thr Arg Gln Asn Val Glu Gly Ser Tyr Asp Gly Ala Tyr Ala

1250 1255 1260

Pro Val Leu Gln Asp Phe Arg Ser Leu Asn Asp Ser Thr Asn Arg Thr

1265 1270 1275 1280

Lys Lys His Thr Ala His Phe Ser Lys Lys Gly Glu Glu Glu Asn Leu

1285 1290 1295

Glu Gly Leu Gly Asn Gln Thr Lys Gln Ile Val Glu Lys Tyr Ala Cys

1300 1305 1310

Thr Thr Arg Ile Ser Pro Asn Thr Ser Gln Gln Asn Phe Val Thr Gln

1315 1320 1325

Arg Ser Lys Arg Ala Leu Lys Gln Phe Arg Leu Pro Leu Glu Glu Thr

1330 1335 1340

Glu Leu Glu Lys Arg Ile Ile Val Asp Asp Thr Ser Thr Gln Trp Ser

1345 1350 1355 1360

Lys Asn Met Lys His Leu Thr Pro Ser Thr Leu Thr Gln Ile Asp Tyr

1365 1370 1375

Asn Glu Lys Glu Lys Gly Ala Ile Thr Gln Ser Pro Leu Ser Asp Cys

1380 1385 1390

Leu Thr Arg Ser His Ser Ile Pro Gln Ala Asn Arg Ser Pro Leu Pro

1395 1400 1405

Ile Ala Lys Val Ser Ser Phe Pro Ser Ile Arg Pro Ile Tyr Leu Thr

1410 1415 1420

Arg Val Leu Phe Gln Asp Asn Ser Ser His Leu Pro Ala Ala Ser Tyr

1425 1430 1435 1440

Arg Lys Lys Asp Ser Gly Val Gln Glu Ser Ser His Phe Leu Gln Gly

1445 1450 1455

Ala Lys Lys Asn Asn Leu Ser Leu Ala Ile Leu Thr Leu Glu Met Thr

1460 1465 1470

Gly Asp Gln Arg Glu Val Gly Ser Leu Gly Thr Ser Ala Thr Asn Ser

1475 1480 1485

Val Thr Tyr Lys Lys Val Glu Asn Thr Val Leu Pro Lys Pro Asp Leu

1490 1495 1500

Pro Lys Thr Ser Gly Lys Val Glu Leu Leu Pro Lys Val His Ile Tyr

1505 1510 1515 1520

Gln Lys Asp Leu Phe Pro Thr Glu Thr Ser Asn Gly Ser Pro Gly His

1525 1530 1535

Leu Asp Leu Val Glu Gly Ser Leu Leu Gln Gly Thr Glu Gly Ala Ile

1540 1545 1550

Lys Trp Asn Glu Ala Asn Arg Pro Gly Lys Val Pro Phe Leu Arg Val

1555 1560 1565

Ala Thr Glu Ser Ser Ala Lys Thr Pro Ser Lys Leu Leu Asp Pro Leu

1570 1575 1580

Ala Trp Asp Asn His Tyr Gly Thr Gln Ile Pro Lys Glu Glu Trp Lys

1585 1590 1595 1600

Ser Gln Glu Lys Ser Pro Glu Lys Thr Ala Phe Lys Lys Lys Asp Thr

1605 1610 1615

Ile Leu Ser Leu Asn Ala Cys Glu Ser Asn His Ala Ile Ala Ala Ile

1620 1625 1630

Asn Glu Gly Gln Asn Lys Pro Glu Ile Glu Val Thr Trp Ala Lys Gln

1635 1640 1645

Gly Arg Thr Glu Arg Leu Cys Ser Gln Asn Pro Pro Val Leu Lys Arg

1650 1655 1660

His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp Gln Glu Glu

1665 1670 1675 1680

Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys Glu Asp Phe

1685 1690 1695

Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser Phe Gln Lys

1700 1705 1710

Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu Trp Asp Tyr

1715 1720 1725

Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala Gln Ser Gly

1730 1735 1740

Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe Thr Asp Gly

1745 1750 1755 1760

Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu His Leu Gly

1765 1770 1775

Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn Ile Met Val

1780 1785 1790

Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr Ser Ser Leu

1795 1800 1805

Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro Arg Lys Asn

1810 1815 1820

Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys Val Gln His

1825 1830 1835 1840

His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala Trp Ala Tyr

1845 1850 1855

Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly Leu Ile Gly

1860 1865 1870

Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala His Gly Arg

1875 1880 1885

Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile Phe Asp Glu

1890 1895 1900

Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn Cys Arg Ala

1905 1910 1915 1920

Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu Asn Tyr Arg

1925 1930 1935

Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro Gly Leu Val

1940 1945 1950

Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser Met Gly Ser

1955 1960 1965

Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His Val Phe Thr Val

1970 1975 1980

Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu Tyr Pro Gly

1985 1990 1995 2000

Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly Ile Trp Arg

2005 2010 2015

Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly Met Ser Thr Leu

2020 2025 2030

Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly Met Ala Ser

2035 2040 2045

Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln Tyr Gly Gln

2050 2055 2060

Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser Ile Asn Ala

2065 2070 2075 2080

Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp Leu Leu Ala

2085 2090 2095

Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg Gln Lys Phe

2100 2105 2110

Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser Leu Asp Gly

2115 2120 2125

Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr Leu Met Val

2130 2135 2140

Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn Ile Phe Asn

2145 2150 2155 2160

Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr His Tyr Ser

2165 2170 2175

Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp Leu Asn Ser

2180 2185 2190

Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser Asp Ala Gln

2195 2200 2205

Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr Trp Ser Pro

2210 2215 2220

Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala Trp Arg Pro

2225 2230 2235 2240

Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe Gln Lys Thr

2245 2250 2255

Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser Leu Leu Thr

2260 2265 2270

Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln Asp Gly His

2275 2280 2285

Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val Phe Gln Gly

2290 2295 2300

Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp Pro Pro Leu

2305 2310 2315 2320

Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val His Gln Ile

2325 2330 2335

Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp Leu Tyr

2340 2345 2350

<210> 22

<211> 1457

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 22

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe

1 5 10 15

Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg

35 40 45

Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val

50 55 60

Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile

65 70 75 80

Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln

85 90 95

Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser

100 105 110

His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser

115 120 125

Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp

130 135 140

Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu

145 150 155 160

Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser

165 170 175

Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile

180 185 190

Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr

195 200 205

Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly

210 215 220

Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp

225 230 235 240

Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr

245 250 255

Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val

260 265 270

Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile

275 280 285

Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser

290 295 300

Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met

305 310 315 320

Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His

325 330 335

Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro

340 345 350

Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp

355 360 365

Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser

370 375 380

Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn

420 425 430

Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met

435 440 445

Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys

500 505 510

Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp

725 730 735

Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys

740 745 750

Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Pro Pro Val Leu

755 760 765

Lys Arg His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp Gln

770 775 780

Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys Glu

785 790 795 800

Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser Phe

805 810 815

Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu Trp

820 825 830

Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala Gln

835 840 845

Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe Thr

850 855 860

Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu His

865 870 875 880

Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn Ile

885 890 895

Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr Ser

900 905 910

Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro Arg

915 920 925

Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys Val

930 935 940

Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala Trp

945 950 955 960

Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly Leu

965 970 975

Ile Gly Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala His

980 985 990

Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile Phe

995 1000 1005

Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn Cys

1010 1015 1020

Arg Ala Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu Asn

1025 1030 1035 1040

Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro Gly

1045 1050 1055

Leu Val Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser Met

1060 1065 1070

Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His Val Phe

1075 1080 1085

Thr Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu Tyr

1090 1095 1100

Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly Ile

1105 1110 1115 1120

Trp Arg Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly Met Ser

1125 1130 1135

Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly Met

1140 1145 1150

Ala Ser Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln Tyr

1155 1160 1165

Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser Ile

1170 1175 1180

Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp Leu

1185 1190 1195 1200

Leu Ala Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg Gln

1205 1210 1215

Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser Leu

1220 1225 1230

Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr Leu

1235 1240 1245

Met Val Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn Ile

1250 1255 1260

Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr His

1265 1270 1275 1280

Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp Leu

1285 1290 1295

Asn Ser Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser Asp

1300 1305 1310

Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr Trp

1315 1320 1325

Ser Pro Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala Trp

1330 1335 1340

Arg Pro Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe Gln

1345 1350 1355 1360

Lys Thr Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser Leu

1365 1370 1375

Leu Thr Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln Asp

1380 1385 1390

Gly His Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val Phe

1395 1400 1405

Gln Gly Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp Pro

1410 1415 1420

Pro Leu Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val His

1425 1430 1435 1440

Gln Ile Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp Leu

1445 1450 1455

Tyr

<210> 23

<211> 1464

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 23

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe

1 5 10 15

Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg

35 40 45

Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val

50 55 60

Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile

65 70 75 80

Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln

85 90 95

Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser

100 105 110

His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser

115 120 125

Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp

130 135 140

Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu

145 150 155 160

Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser

165 170 175

Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile

180 185 190

Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr

195 200 205

Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly

210 215 220

Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp

225 230 235 240

Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr

245 250 255

Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val

260 265 270

Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile

275 280 285

Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser

290 295 300

Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met

305 310 315 320

Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His

325 330 335

Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro

340 345 350

Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp

355 360 365

Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser

370 375 380

Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn

420 425 430

Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met

435 440 445

Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys

500 505 510

Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp

725 730 735

Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys

740 745 750

Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Pro

755 760 765

Ser Gln Asn Pro Pro Val Leu Lys Arg His Gln Arg Glu Ile Thr Arg

770 775 780

Thr Thr Leu Gln Ser Asp Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile

785 790 795 800

Ser Val Glu Met Lys Lys Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu

805 810 815

Asn Gln Ser Pro Arg Ser Phe Gln Lys Lys Thr Arg His Tyr Phe Ile

820 825 830

Ala Ala Val Glu Arg Leu Trp Asp Tyr Gly Met Ser Ser Ser Pro His

835 840 845

Val Leu Arg Asn Arg Ala Gln Ser Gly Ser Val Pro Gln Phe Lys Lys

850 855 860

Val Val Phe Gln Glu Phe Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr

865 870 875 880

Arg Gly Glu Leu Asn Glu His Leu Gly Leu Leu Gly Pro Tyr Ile Arg

885 890 895

Ala Glu Val Glu Asp Asn Ile Met Val Thr Phe Arg Asn Gln Ala Ser

900 905 910

Arg Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln

915 920 925

Arg Gln Gly Ala Glu Pro Arg Lys Asn Phe Val Lys Pro Asn Glu Thr

930 935 940

Lys Thr Tyr Phe Trp Lys Val Gln His His Met Ala Pro Thr Lys Asp

945 950 955 960

Glu Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu

965 970 975

Lys Asp Val His Ser Gly Leu Ile Gly Pro Leu Leu Val Cys His Thr

980 985 990

Asn Thr Leu Asn Pro Ala His Gly Arg Gln Val Thr Val Gln Glu Phe

995 1000 1005

Ala Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr

1010 1015 1020

Glu Asn Met Glu Arg Asn Cys Arg Ala Pro Cys Asn Ile Gln Met Glu

1025 1030 1035 1040

Asp Pro Thr Phe Lys Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr

1045 1050 1055

Ile Met Asp Thr Leu Pro Gly Leu Val Met Ala Gln Asp Gln Arg Ile

1060 1065 1070

Arg Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile

1075 1080 1085

His Phe Ser Gly His Val Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys

1090 1095 1100

Met Ala Leu Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met

1105 1110 1115 1120

Leu Pro Ser Lys Ala Gly Ile Trp Arg Val Glu Cys Leu Ile Gly Glu

1125 1130 1135

His Leu His Ala Gly Met Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys

1140 1145 1150

Cys Gln Thr Pro Leu Gly Met Ala Ser Gly His Ile Arg Asp Phe Gln

1155 1160 1165

Ile Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg

1170 1175 1180

Leu His Tyr Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe

1185 1190 1195 1200

Ser Trp Ile Lys Val Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile

1205 1210 1215

Lys Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln

1220 1225 1230

Phe Ile Ile Met Tyr Ser Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg

1235 1240 1245

Gly Asn Ser Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val Asp Ser

1250 1255 1260

Ser Gly Ile Lys His Asn Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr

1265 1270 1275 1280

Ile Arg Leu His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met

1285 1290 1295

Glu Leu Met Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met

1300 1305 1310

Glu Ser Lys Ala Ile Ser Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe

1315 1320 1325

Thr Asn Met Phe Ala Thr Trp Ser Pro Ser Lys Ala Arg Leu His Leu

1330 1335 1340

Gln Gly Arg Ser Asn Ala Trp Arg Pro Gln Val Asn Asn Pro Lys Glu

1345 1350 1355 1360

Trp Leu Gln Val Asp Phe Gln Lys Thr Met Lys Val Thr Gly Val Thr

1365 1370 1375

Thr Gln Gly Val Lys Ser Leu Leu Thr Ser Met Tyr Val Lys Glu Phe

1380 1385 1390

Leu Ile Ser Ser Ser Gln Asp Gly His Gln Trp Thr Leu Phe Phe Gln

1395 1400 1405

Asn Gly Lys Val Lys Val Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro

1410 1415 1420

Val Val Asn Ser Leu Asp Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile

1425 1430 1435 1440

His Pro Gln Ser Trp Val His Gln Ile Ala Leu Arg Met Glu Val Leu

1445 1450 1455

Gly Cys Glu Ala Gln Asp Leu Tyr

1460

<210> 24

<211> 1463

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 24

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe

1 5 10 15

Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg

35 40 45

Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val

50 55 60

Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile

65 70 75 80

Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln

85 90 95

Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser

100 105 110

His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser

115 120 125

Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp

130 135 140

Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu

145 150 155 160

Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser

165 170 175

Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile

180 185 190

Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr

195 200 205

Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly

210 215 220

Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp

225 230 235 240

Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr

245 250 255

Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val

260 265 270

Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile

275 280 285

Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser

290 295 300

Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met

305 310 315 320

Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His

325 330 335

Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro

340 345 350

Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp

355 360 365

Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser

370 375 380

Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn

420 425 430

Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met

435 440 445

Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys

500 505 510

Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp

725 730 735

Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys

740 745 750

Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Pro

755 760 765

Ser Thr Arg Gln Lys Gln Phe Asn Ala Thr Thr Ile Pro Glu Asn Thr

770 775 780

Thr Leu Gln Ser Asp Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser

785 790 795 800

Val Glu Met Lys Lys Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn

805 810 815

Gln Ser Pro Arg Ser Phe Gln Lys Lys Thr Arg His Tyr Phe Ile Ala

820 825 830

Ala Val Glu Arg Leu Trp Asp Tyr Gly Met Ser Ser Ser Pro His Val

835 840 845

Leu Arg Asn Arg Ala Gln Ser Gly Ser Val Pro Gln Phe Lys Lys Val

850 855 860

Val Phe Gln Glu Phe Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg

865 870 875 880

Gly Glu Leu Asn Glu His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala

885 890 895

Glu Val Glu Asp Asn Ile Met Val Thr Phe Arg Asn Gln Ala Ser Arg

900 905 910

Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg

915 920 925

Gln Gly Ala Glu Pro Arg Lys Asn Phe Val Lys Pro Asn Glu Thr Lys

930 935 940

Thr Tyr Phe Trp Lys Val Gln His His Met Ala Pro Thr Lys Asp Glu

945 950 955 960

Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys

965 970 975

Asp Val His Ser Gly Leu Ile Gly Pro Leu Leu Val Cys His Thr Asn

980 985 990

Thr Leu Asn Pro Ala His Gly Arg Gln Val Thr Val Gln Glu Phe Ala

995 1000 1005

Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu

1010 1015 1020

Asn Met Glu Arg Asn Cys Arg Ala Pro Cys Asn Ile Gln Met Glu Asp

1025 1030 1035 1040

Pro Thr Phe Lys Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile

1045 1050 1055

Met Asp Thr Leu Pro Gly Leu Val Met Ala Gln Asp Gln Arg Ile Arg

1060 1065 1070

Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile His

1075 1080 1085

Phe Ser Gly His Val Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys Met

1090 1095 1100

Ala Leu Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu

1105 1110 1115 1120

Pro Ser Lys Ala Gly Ile Trp Arg Val Glu Cys Leu Ile Gly Glu His

1125 1130 1135

Leu His Ala Gly Met Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys

1140 1145 1150

Gln Thr Pro Leu Gly Met Ala Ser Gly His Ile Arg Asp Phe Gln Ile

1155 1160 1165

Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu

1170 1175 1180

His Tyr Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser

1185 1190 1195 1200

Trp Ile Lys Val Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile Lys

1205 1210 1215

Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe

1220 1225 1230

Ile Ile Met Tyr Ser Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly

1235 1240 1245

Asn Ser Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val Asp Ser Ser

1250 1255 1260

Gly Ile Lys His Asn Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile

1265 1270 1275 1280

Arg Leu His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu

1285 1290 1295

Leu Met Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met Glu

1300 1305 1310

Ser Lys Ala Ile Ser Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr

1315 1320 1325

Asn Met Phe Ala Thr Trp Ser Pro Ser Lys Ala Arg Leu His Leu Gln

1330 1335 1340

Gly Arg Ser Asn Ala Trp Arg Pro Gln Val Asn Asn Pro Lys Glu Trp

1345 1350 1355 1360

Leu Gln Val Asp Phe Gln Lys Thr Met Lys Val Thr Gly Val Thr Thr

1365 1370 1375

Gln Gly Val Lys Ser Leu Leu Thr Ser Met Tyr Val Lys Glu Phe Leu

1380 1385 1390

Ile Ser Ser Ser Gln Asp Gly His Gln Trp Thr Leu Phe Phe Gln Asn

1395 1400 1405

Gly Lys Val Lys Val Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro Val

1410 1415 1420

Val Asn Ser Leu Asp Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile His

1425 1430 1435 1440

Pro Gln Ser Trp Val His Gln Ile Ala Leu Arg Met Glu Val Leu Gly

1445 1450 1455

Cys Glu Ala Gln Asp Leu Tyr

1460

<210> 25

<211> 1453

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 25

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe

1 5 10 15

Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg

35 40 45

Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val

50 55 60

Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile

65 70 75 80

Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln

85 90 95

Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser

100 105 110

His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser

115 120 125

Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp

130 135 140

Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu

145 150 155 160

Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser

165 170 175

Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile

180 185 190

Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr

195 200 205

Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly

210 215 220

Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp

225 230 235 240

Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr

245 250 255

Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val

260 265 270

Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile

275 280 285

Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser

290 295 300

Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met

305 310 315 320

Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His

325 330 335

Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro

340 345 350

Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp

355 360 365

Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser

370 375 380

Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn

420 425 430

Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met

435 440 445

Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys

500 505 510

Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp

725 730 735

Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys

740 745 750

Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Pro Pro Val Leu

755 760 765

Lys Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp Gln Glu Glu Ile Asp

770 775 780

Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys Glu Asp Phe Asp Ile

785 790 795 800

Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser Phe Gln Lys Lys Thr

805 810 815

Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu Trp Asp Tyr Gly Met

820 825 830

Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala Gln Ser Gly Ser Val

835 840 845

Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe Thr Asp Gly Ser Phe

850 855 860

Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu His Leu Gly Leu Leu

865 870 875 880

Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn Ile Met Val Thr Phe

885 890 895

Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser

900 905 910

Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro Arg Lys Asn Phe Val

915 920 925

Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys Val Gln His His Met

930 935 940

Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser

945 950 955 960

Asp Val Asp Leu Glu Lys Asp Val His Ser Gly Leu Ile Gly Pro Leu

965 970 975

Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala His Gly Arg Gln Val

980 985 990

Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys

995 1000 1005

Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn Cys Arg Ala Pro Cys

1010 1015 1020

Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu Asn Tyr Arg Phe His

1025 1030 1035 1040

Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro Gly Leu Val Met Ala

1045 1050 1055

Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu

1060 1065 1070

Asn Ile His Ser Ile His Phe Ser Gly His Val Phe Thr Val Arg Lys

1075 1080 1085

Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu Tyr Pro Gly Val Phe

1090 1095 1100

Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly Ile Trp Arg Val Glu

1105 1110 1115 1120

Cys Leu Ile Gly Glu His Leu His Ala Gly Met Ser Thr Leu Phe Leu

1125 1130 1135

Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly Met Ala Ser Gly His

1140 1145 1150

Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala

1155 1160 1165

Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser Ile Asn Ala Trp Ser

1170 1175 1180

Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp Leu Leu Ala Pro Met

1185 1190 1195 1200

Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser

1205 1210 1215

Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser Leu Asp Gly Lys Lys

1220 1225 1230

Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr Leu Met Val Phe Phe

1235 1240 1245

Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn Ile Phe Asn Pro Pro

1250 1255 1260

Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr His Tyr Ser Ile Arg

1265 1270 1275 1280

Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp Leu Asn Ser Cys Ser

1285 1290 1295

Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser Asp Ala Gln Ile Thr

1300 1305 1310

Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr Trp Ser Pro Ser Lys

1315 1320 1325

Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala Trp Arg Pro Gln Val

1330 1335 1340

Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe Gln Lys Thr Met Lys

1345 1350 1355 1360

Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser Leu Leu Thr Ser Met

1365 1370 1375

Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln Asp Gly His Gln Trp

1380 1385 1390

Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val Phe Gln Gly Asn Gln

1395 1400 1405

Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp Pro Pro Leu Leu Thr

1410 1415 1420

Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val His Gln Ile Ala Leu

1425 1430 1435 1440

Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp Leu Tyr

1445 1450

<210> 26

<211> 1474

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 26

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe

1 5 10 15

Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg

35 40 45

Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val

50 55 60

Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile

65 70 75 80

Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln

85 90 95

Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser

100 105 110

His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser

115 120 125

Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp

130 135 140

Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu

145 150 155 160

Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser

165 170 175

Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile

180 185 190

Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr

195 200 205

Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly

210 215 220

Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp

225 230 235 240

Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr

245 250 255

Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val

260 265 270

Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile

275 280 285

Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser

290 295 300

Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met

305 310 315 320

Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His

325 330 335

Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro

340 345 350

Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp

355 360 365

Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser

370 375 380

Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn

420 425 430

Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met

435 440 445

Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys

500 505 510

Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp

725 730 735

Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys

740 745 750

Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ala Thr Asn Val

755 760 765

Ser Asn Asn Ser Asn Thr Ser Asn Asp Ser Asn Val Ser Pro Pro Val

770 775 780

Leu Lys Arg His Gln Arg Glu Ile Thr Arg Thr Thr Leu Gln Ser Asp

785 790 795 800

Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys Lys

805 810 815

Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg Ser

820 825 830

Phe Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg Leu

835 840 845

Trp Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg Ala

850 855 860

Gln Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu Phe

865 870 875 880

Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn Glu

885 890 895

His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp Asn

900 905 910

Ile Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe Tyr

915 920 925

Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu Pro

930 935 940

Arg Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp Lys

945 950 955 960

Val Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys Ala

965 970 975

Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser Gly

980 985 990

Leu Ile Gly Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro Ala

995 1000 1005

His Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr Ile

1010 1015 1020

Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg Asn

1025 1030 1035 1040

Cys Arg Ala Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys Glu

1045 1050 1055

Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu Pro

1060 1065 1070

Gly Leu Val Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu Ser

1075 1080 1085

Met Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His Val

1090 1095 1100

Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn Leu

1105 1110 1115 1120

Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala Gly

1125 1130 1135

Ile Trp Arg Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly Met

1140 1145 1150

Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu Gly

1155 1160 1165

Met Ala Ser Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly Gln

1170 1175 1180

Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly Ser

1185 1190 1195 1200

Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val Asp

1205 1210 1215

Leu Leu Ala Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala Arg

1220 1225 1230

Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr Ser

1235 1240 1245

Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly Thr

1250 1255 1260

Leu Met Val Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His Asn

1265 1270 1275 1280

Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro Thr

1285 1290 1295

His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys Asp

1300 1305 1310

Leu Asn Ser Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile Ser

1315 1320 1325

Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala Thr

1330 1335 1340

Trp Ser Pro Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn Ala

1345 1350 1355 1360

Trp Arg Pro Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp Phe

1365 1370 1375

Gln Lys Thr Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys Ser

1380 1385 1390

Leu Leu Thr Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser Gln

1395 1400 1405

Asp Gly His Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys Val

1410 1415 1420

Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu Asp

1425 1430 1435 1440

Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp Val

1445 1450 1455

His Gln Ile Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln Asp

1460 1465 1470

Leu Tyr

<210> 27

<211> 1459

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 27

Met Gln Ile Glu Leu Ser Thr Cys Phe Phe Leu Cys Leu Leu Arg Phe

1 5 10 15

Cys Phe Ser Ala Thr Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Met Gln Ser Asp Leu Gly Glu Leu Pro Val Asp Ala Arg

35 40 45

Phe Pro Pro Arg Val Pro Lys Ser Phe Pro Phe Asn Thr Ser Val Val

50 55 60

Tyr Lys Lys Thr Leu Phe Val Glu Phe Thr Asp His Leu Phe Asn Ile

65 70 75 80

Ala Lys Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile Gln

85 90 95

Ala Glu Val Tyr Asp Thr Val Val Ile Thr Leu Lys Asn Met Ala Ser

100 105 110

His Pro Val Ser Leu His Ala Val Gly Val Ser Tyr Trp Lys Ala Ser

115 120 125

Glu Gly Ala Glu Tyr Asp Asp Gln Thr Ser Gln Arg Glu Lys Glu Asp

130 135 140

Asp Lys Val Phe Pro Gly Gly Ser His Thr Tyr Val Trp Gln Val Leu

145 150 155 160

Lys Glu Asn Gly Pro Met Ala Ser Asp Pro Leu Cys Leu Thr Tyr Ser

165 170 175

Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu Ile

180 185 190

Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Ala Lys Glu Lys Thr

195 200 205

Gln Thr Leu His Lys Phe Ile Leu Leu Phe Ala Val Phe Asp Glu Gly

210 215 220

Lys Ser Trp His Ser Glu Thr Lys Asn Ser Leu Met Gln Asp Arg Asp

225 230 235 240

Ala Ala Ser Ala Arg Ala Trp Pro Lys Met His Thr Val Asn Gly Tyr

245 250 255

Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Arg Lys Ser Val

260 265 270

Tyr Trp His Val Ile Gly Met Gly Thr Thr Pro Glu Val His Ser Ile

275 280 285

Phe Leu Glu Gly His Thr Phe Leu Val Arg Asn His Arg Gln Ala Ser

290 295 300

Leu Glu Ile Ser Pro Ile Thr Phe Leu Thr Ala Gln Thr Leu Leu Met

305 310 315 320

Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His Gln His

325 330 335

Asp Gly Met Glu Ala Tyr Val Lys Val Asp Ser Cys Pro Glu Glu Pro

340 345 350

Gln Leu Arg Met Lys Asn Asn Glu Glu Ala Glu Asp Tyr Asp Asp Asp

355 360 365

Leu Thr Asp Ser Glu Met Asp Val Val Arg Phe Asp Asp Asp Asn Ser

370 375 380

Pro Ser Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ala Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Leu Val Leu Ala Pro Asp Asp Arg Ser Tyr Lys Ser Gln Tyr Leu Asn

420 425 430

Asn Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Val Arg Phe Met

435 440 445

Ala Tyr Thr Asp Glu Thr Phe Lys Thr Arg Glu Ala Ile Gln His Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Gln Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Arg Pro Leu Tyr Ser Arg Arg Leu Pro Lys

500 505 510

Gly Val Lys His Leu Lys Asp Phe Pro Ile Leu Pro Gly Glu Ile Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Phe Val Asn Met Glu Arg

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Ile Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Arg Ser Trp Tyr Leu Thr Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Ala Gly Val Gln Leu Glu Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Ile Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Ile Leu Ala Cys His Asn Ser Asp Phe Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Ser Ser Cys Asp Lys Asn Thr Gly Asp

725 730 735

Tyr Tyr Glu Asp Ser Tyr Glu Asp Ile Ser Ala Tyr Leu Leu Ser Lys

740 745 750

Asn Asn Ala Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg His Gln

755 760 765

Ala Tyr Arg Tyr Arg Arg Gly Glu Ile Thr Arg Thr Thr Leu Gln Ser

770 775 780

Asp Gln Glu Glu Ile Asp Tyr Asp Asp Thr Ile Ser Val Glu Met Lys

785 790 795 800

Lys Glu Asp Phe Asp Ile Tyr Asp Glu Asp Glu Asn Gln Ser Pro Arg

805 810 815

Ser Phe Gln Lys Lys Thr Arg His Tyr Phe Ile Ala Ala Val Glu Arg

820 825 830

Leu Trp Asp Tyr Gly Met Ser Ser Ser Pro His Val Leu Arg Asn Arg

835 840 845

Ala Gln Ser Gly Ser Val Pro Gln Phe Lys Lys Val Val Phe Gln Glu

850 855 860

Phe Thr Asp Gly Ser Phe Thr Gln Pro Leu Tyr Arg Gly Glu Leu Asn

865 870 875 880

Glu His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val Glu Asp

885 890 895

Asn Ile Met Val Thr Phe Arg Asn Gln Ala Ser Arg Pro Tyr Ser Phe

900 905 910

Tyr Ser Ser Leu Ile Ser Tyr Glu Glu Asp Gln Arg Gln Gly Ala Glu

915 920 925

Pro Arg Lys Asn Phe Val Lys Pro Asn Glu Thr Lys Thr Tyr Phe Trp

930 935 940

Lys Val Gln His His Met Ala Pro Thr Lys Asp Glu Phe Asp Cys Lys

945 950 955 960

Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val His Ser

965 970 975

Gly Leu Ile Gly Pro Leu Leu Val Cys His Thr Asn Thr Leu Asn Pro

980 985 990

Ala His Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe Phe Thr

995 1000 1005

Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Met Glu Arg

1010 1015 1020

Asn Cys Arg Ala Pro Cys Asn Ile Gln Met Glu Asp Pro Thr Phe Lys

1025 1030 1035 1040

Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Ile Met Asp Thr Leu

1045 1050 1055

Pro Gly Leu Val Met Ala Gln Asp Gln Arg Ile Arg Trp Tyr Leu Leu

1060 1065 1070

Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser Gly His

1075 1080 1085

Val Phe Thr Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Leu Tyr Asn

1090 1095 1100

Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser Lys Ala

1105 1110 1115 1120

Gly Ile Trp Arg Val Glu Cys Leu Ile Gly Glu His Leu His Ala Gly

1125 1130 1135

Met Ser Thr Leu Phe Leu Val Tyr Ser Asn Lys Cys Gln Thr Pro Leu

1140 1145 1150

Gly Met Ala Ser Gly His Ile Arg Asp Phe Gln Ile Thr Ala Ser Gly

1155 1160 1165

Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr Ser Gly

1170 1175 1180

Ser Ile Asn Ala Trp Ser Thr Lys Glu Pro Phe Ser Trp Ile Lys Val

1185 1190 1195 1200

Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile Lys Thr Gln Gly Ala

1205 1210 1215

Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile Met Tyr

1220 1225 1230

Ser Leu Asp Gly Lys Lys Trp Gln Thr Tyr Arg Gly Asn Ser Thr Gly

1235 1240 1245

Thr Leu Met Val Phe Phe Gly Asn Val Asp Ser Ser Gly Ile Lys His

1250 1255 1260

Asn Ile Phe Asn Pro Pro Ile Ile Ala Arg Tyr Ile Arg Leu His Pro

1265 1270 1275 1280

Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met Gly Cys

1285 1290 1295

Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met Glu Ser Lys Ala Ile

1300 1305 1310

Ser Asp Ala Gln Ile Thr Ala Ser Ser Tyr Phe Thr Asn Met Phe Ala

1315 1320 1325

Thr Trp Ser Pro Ser Lys Ala Arg Leu His Leu Gln Gly Arg Ser Asn

1330 1335 1340

Ala Trp Arg Pro Gln Val Asn Asn Pro Lys Glu Trp Leu Gln Val Asp

1345 1350 1355 1360

Phe Gln Lys Thr Met Lys Val Thr Gly Val Thr Thr Gln Gly Val Lys

1365 1370 1375

Ser Leu Leu Thr Ser Met Tyr Val Lys Glu Phe Leu Ile Ser Ser Ser

1380 1385 1390

Gln Asp Gly His Gln Trp Thr Leu Phe Phe Gln Asn Gly Lys Val Lys

1395 1400 1405

Val Phe Gln Gly Asn Gln Asp Ser Phe Thr Pro Val Val Asn Ser Leu

1410 1415 1420

Asp Pro Pro Leu Leu Thr Arg Tyr Leu Arg Ile His Pro Gln Ser Trp

1425 1430 1435 1440

Val His Gln Ile Ala Leu Arg Met Glu Val Leu Gly Cys Glu Ala Gln

1445 1450 1455

Asp Leu Tyr

<210> 28

<211> 2133

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 28

Met Gln Leu Glu Leu Ser Thr Cys Val Phe Leu Cys Leu Leu Pro Leu

1 5 10 15

Gly Phe Ser Ala Ile Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Arg Gln Ser Glu Leu Leu Arg Glu Leu His Val Asp Thr

35 40 45

Arg Phe Pro Ala Thr Ala Pro Gly Ala Leu Pro Leu Gly Pro Ser Val

50 55 60

Leu Tyr Lys Lys Thr Val Phe Val Glu Phe Thr Asp Gln Leu Phe Ser

65 70 75 80

Val Ala Arg Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile

85 90 95

Gln Ala Glu Val Tyr Asp Thr Val Val Val Thr Leu Lys Asn Met Ala

100 105 110

Ser His Pro Val Ser Leu His Ala Val Gly Val Ser Phe Trp Lys Ser

115 120 125

Ser Glu Gly Ala Glu Tyr Glu Asp His Thr Ser Gln Arg Glu Lys Glu

130 135 140

Asp Asp Lys Val Leu Pro Gly Lys Ser Gln Thr Tyr Val Trp Gln Val

145 150 155 160

Leu Lys Glu Asn Gly Pro Thr Ala Ser Asp Pro Pro Cys Leu Thr Tyr

165 170 175

Ser Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu

180 185 190

Ile Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Thr Arg Glu Arg

195 200 205

Thr Gln Asn Leu His Glu Phe Val Leu Leu Phe Ala Val Phe Asp Glu

210 215 220

Gly Lys Ser Trp His Ser Ala Arg Asn Asp Ser Trp Thr Arg Ala Met

225 230 235 240

Asp Pro Ala Pro Ala Arg Ala Gln Pro Ala Met His Thr Val Asn Gly

245 250 255

Tyr Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Lys Lys Ser

260 265 270

Val Tyr Trp His Val Ile Gly Met Gly Thr Ser Pro Glu Val His Ser

275 280 285

Ile Phe Leu Glu Gly His Thr Phe Leu Val Arg His His Arg Gln Ala

290 295 300

Ser Leu Glu Ile Ser Pro Leu Thr Phe Leu Thr Ala Gln Thr Phe Leu

305 310 315 320

Met Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His His

325 330 335

His Gly Gly Met Glu Ala His Val Arg Val Glu Ser Cys Ala Glu Glu

340 345 350

Pro Gln Leu Arg Arg Lys Ala Asp Glu Glu Glu Asp Tyr Asp Asp Asn

355 360 365

Leu Tyr Asp Ser Asp Met Asp Val Val Arg Leu Asp Gly Asp Asp Val

370 375 380

Ser Pro Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ser Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Ala Val Pro Ser Pro Ser Asp Arg Ser Tyr Lys Ser Leu Tyr Leu Asn

420 425 430

Ser Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Ala Arg Phe Val

435 440 445

Ala Tyr Thr Asp Val Thr Phe Lys Thr Arg Lys Ala Ile Pro Tyr Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Lys Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Ser Ala Leu His Pro Gly Arg Leu Leu Lys

500 505 510

Gly Trp Lys His Leu Lys Asp Met Pro Ile Leu Pro Gly Glu Thr Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Ser Ile Asn Leu Glu Lys

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Met Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Gln Ser Trp Tyr Leu Ala Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Asp Gly Leu Gln Pro Gln Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Val Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Val Leu Ala Cys His Asn Ser Asp Leu Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Tyr Ser Cys Asp Arg Asp Ile Gly Asp

725 730 735

Tyr Tyr Asp Asn Thr Tyr Glu Asp Ile Pro Gly Phe Leu Leu Ser Gly

740 745 750

Lys Asn Val Ile Glu Pro Arg Ser Phe Ala Gln Asn Ser Arg Pro Pro

755 760 765

Ser Ala Ser Gln Lys Gln Phe Gln Thr Ile Thr Ser Pro Glu Asp Asp

770 775 780

Val Glu Leu Asp Pro Gln Ser Gly Glu Arg Thr Gln Ala Leu Glu Glu

785 790 795 800

Leu Ser Val Pro Ser Gly Asp Gly Ser Met Leu Leu Gly Gln Asn Pro

805 810 815

Ala Pro His Gly Ser Ser Ser Ser Asp Leu Gln Glu Ala Arg Asn Glu

820 825 830

Ala Asp Asp Tyr Leu Pro Gly Ala Arg Glu Arg Asn Thr Ala Pro Ser

835 840 845

Ala Ala Ala Arg Leu Arg Pro Glu Leu His His Ser Ala Glu Arg Val

850 855 860

Leu Thr Pro Glu Pro Glu Lys Glu Leu Lys Lys Leu Asp Ser Lys Met

865 870 875 880

Ser Ser Ser Ser Asp Leu Leu Lys Thr Ser Pro Thr Ile Pro Ser Asp

885 890 895

Thr Leu Ser Ala Glu Thr Glu Arg Thr His Ser Leu Gly Pro Pro His

900 905 910

Pro Gln Val Asn Phe Arg Ser Gln Leu Gly Ala Ile Val Leu Gly Lys

915 920 925

Asn Ser Ser His Phe Ile Gly Ala Gly Val Pro Leu Gly Ser Thr Glu

930 935 940

Glu Asp His Glu Ser Ser Leu Gly Glu Asn Val Ser Pro Val Glu Ser

945 950 955 960

Asp Gly Ile Phe Glu Lys Glu Arg Ala His Gly Pro Ala Ser Leu Thr

965 970 975

Lys Asp Asp Val Leu Phe Lys Val Asn Ile Ser Leu Val Lys Thr Asn

980 985 990

Lys Ala Arg Val Tyr Leu Lys Thr Asn Arg Lys Ile His Ile Asp Asp

995 1000 1005

Ala Ala Leu Leu Thr Glu Asn Arg Ala Ser Ala Thr Phe Met Asp Lys

1010 1015 1020

Asn Thr Thr Ala Ser Gly Leu Asn His Val Ser Asn Trp Ile Lys Gly

1025 1030 1035 1040

Pro Leu Gly Lys Asn Pro Leu Ser Ser Glu Arg Gly Pro Ser Pro Glu

1045 1050 1055

Leu Leu Thr Ser Ser Gly Ser Gly Lys Ser Val Lys Gly Gln Ser Ser

1060 1065 1070

Gly Gln Gly Arg Ile Arg Val Ala Val Glu Glu Glu Glu Leu Ser Lys

1075 1080 1085

Gly Lys Glu Met Met Leu Pro Asn Ser Glu Leu Thr Phe Leu Thr Asn

1090 1095 1100

Ser Ala Asp Val Gln Gly Asn Asp Thr His Ser Gln Gly Lys Lys Ser

1105 1110 1115 1120

Arg Glu Glu Met Glu Arg Arg Glu Lys Leu Val Gln Glu Lys Val Asp

1125 1130 1135

Leu Pro Gln Val Tyr Thr Ala Thr Gly Thr Lys Asn Phe Leu Arg Asn

1140 1145 1150

Ile Phe His Gln Ser Thr Glu Pro Ser Val Glu Gly Phe Asp Gly Gly

1155 1160 1165

Ser His Ala Pro Val Pro Gln Asp Ser Arg Ser Leu Asn Asp Ser Ala

1170 1175 1180

Glu Arg Ala Glu Thr His Ile Ala His Phe Ser Ala Ile Arg Glu Glu

1185 1190 1195 1200

Ala Pro Leu Glu Ala Pro Gly Asn Arg Thr Gly Pro Gly Pro Arg Ser

1205 1210 1215

Ala Val Pro Arg Arg Val Lys Gln Ser Leu Lys Gln Ile Arg Leu Pro

1220 1225 1230

Leu Glu Glu Ile Lys Pro Glu Arg Gly Val Val Leu Asn Ala Thr Ser

1235 1240 1245

Thr Arg Trp Ser Glu Ser Ser Pro Ile Leu Gln Gly Ala Lys Arg Asn

1250 1255 1260

Asn Leu Ser Leu Pro Phe Leu Thr Leu Glu Met Ala Gly Gly Gln Gly

1265 1270 1275 1280

Lys Ile Ser Ala Leu Gly Lys Ser Ala Ala Gly Pro Leu Ala Ser Gly

1285 1290 1295

Lys Leu Glu Lys Ala Val Leu Ser Ser Ala Gly Leu Ser Glu Ala Ser

1300 1305 1310

Gly Lys Ala Glu Phe Leu Pro Lys Val Arg Val His Arg Glu Asp Leu

1315 1320 1325

Leu Pro Gln Lys Thr Ser Asn Val Ser Cys Ala His Gly Asp Leu Gly

1330 1335 1340

Gln Glu Ile Phe Leu Gln Lys Thr Arg Gly Pro Val Asn Leu Asn Lys

1345 1350 1355 1360

Val Asn Arg Pro Gly Arg Thr Pro Ser Lys Leu Leu Gly Pro Pro Met

1365 1370 1375

Pro Lys Glu Trp Glu Ser Leu Glu Lys Ser Pro Lys Ser Thr Ala Leu

1380 1385 1390

Arg Thr Lys Asp Ile Ile Ser Leu Pro Leu Asp Arg His Glu Ser Asn

1395 1400 1405

His Ser Ile Ala Ala Lys Asn Glu Gly Gln Ala Glu Thr Gln Arg Glu

1410 1415 1420

Ala Ala Trp Thr Lys Gln Gly Gly Pro Gly Arg Leu Cys Ala Pro Lys

1425 1430 1435 1440

Pro Pro Val Leu Arg Arg His Gln Arg Asp Ile Ser Leu Pro Thr Phe

1445 1450 1455

Gln Pro Glu Glu Asp Lys Met Asp Tyr Asp Asp Ile Phe Ser Thr Glu

1460 1465 1470

Thr Lys Gly Glu Asp Phe Asp Ile Tyr Gly Glu Asp Glu Asn Gln Asp

1475 1480 1485

Pro Arg Ser Phe Gln Lys Arg Thr Arg His Tyr Phe Ile Ala Ala Val

1490 1495 1500

Glu Gln Leu Trp Asp Tyr Gly Met Ser Glu Ser Pro Arg Ala Leu Arg

1505 1510 1515 1520

Asn Arg Ala Gln Asn Gly Glu Val Pro Arg Phe Lys Lys Val Val Phe

1525 1530 1535

Arg Glu Phe Ala Asp Gly Ser Phe Thr Gln Pro Ser Tyr Arg Gly Glu

1540 1545 1550

Leu Asn Lys His Leu Gly Leu Leu Gly Pro Tyr Ile Arg Ala Glu Val

1555 1560 1565

Glu Asp Asn Ile Met Val Thr Phe Lys Asn Gln Ala Ser Arg Pro Tyr

1570 1575 1580

Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Pro Asp Asp Gln Glu Gln Gly

1585 1590 1595 1600

Ala Glu Pro Arg His Asn Phe Val Gln Pro Asn Glu Thr Arg Thr Tyr

1605 1610 1615

Phe Trp Lys Val Gln His His Met Ala Pro Thr Glu Asp Glu Phe Asp

1620 1625 1630

Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp Leu Glu Lys Asp Val

1635 1640 1645

His Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Arg Ala Asn Thr Leu

1650 1655 1660

Asn Ala Ala His Gly Arg Gln Val Thr Val Gln Glu Phe Ala Leu Phe

1665 1670 1675 1680

Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr Phe Thr Glu Asn Val

1685 1690 1695

Glu Arg Asn Cys Arg Ala Pro Cys His Leu Gln Met Glu Asp Pro Thr

1700 1705 1710

Leu Lys Glu Asn Tyr Arg Phe His Ala Ile Asn Gly Tyr Val Met Asp

1715 1720 1725

Thr Leu Pro Gly Leu Val Met Ala Gln Asn Gln Arg Ile Arg Trp Tyr

1730 1735 1740

Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His Ser Ile His Phe Ser

1745 1750 1755 1760

Gly His Val Phe Ser Val Arg Lys Lys Glu Glu Tyr Lys Met Ala Val

1765 1770 1775

Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val Glu Met Leu Pro Ser

1780 1785 1790

Lys Val Gly Ile Trp Arg Ile Glu Cys Leu Ile Gly Glu His Leu Gln

1795 1800 1805

Ala Gly Met Ser Thr Thr Phe Leu Val Tyr Ser Lys Glu Cys Gln Ala

1810 1815 1820

Pro Leu Gly Met Ala Ser Gly Arg Ile Arg Asp Phe Gln Ile Thr Ala

1825 1830 1835 1840

Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu Ala Arg Leu His Tyr

1845 1850 1855

Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Asp Pro His Ser Trp Ile

1860 1865 1870

Lys Val Asp Leu Leu Ala Pro Met Ile Ile His Gly Ile Met Thr Gln

1875 1880 1885

Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile Ser Gln Phe Ile Ile

1890 1895 1900

Met Tyr Ser Leu Asp Gly Arg Asn Trp Gln Ser Tyr Arg Gly Asn Ser

1905 1910 1915 1920

Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val Asp Ala Ser Gly Ile

1925 1930 1935

Lys His Asn Ile Phe Asn Pro Pro Ile Val Ala Arg Tyr Ile Arg Leu

1940 1945 1950

His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu Arg Met Glu Leu Met

1955 1960 1965

Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu Gly Met Gln Asn Lys

1970 1975 1980

Ala Ile Ser Asp Ser Gln Ile Thr Ala Ser Ser His Leu Ser Asn Ile

1985 1990 1995 2000

Phe Ala Thr Trp Ser Pro Ser Gln Ala Arg Leu His Leu Gln Gly Arg

2005 2010 2015

Thr Asn Ala Trp Arg Pro Arg Val Ser Ser Ala Glu Glu Trp Leu Gln

2020 2025 2030

Val Asp Leu Gln Lys Thr Val Lys Val Thr Gly Ile Thr Thr Gln Gly

2035 2040 2045

Val Lys Ser Leu Leu Ser Ser Met Tyr Val Lys Glu Phe Leu Val Ser

2050 2055 2060

Ser Ser Gln Asp Gly Arg Arg Trp Thr Leu Phe Leu Gln Asp Gly His

2065 2070 2075 2080

Thr Lys Val Phe Gln Gly Asn Gln Asp Ser Ser Thr Pro Val Val Asn

2085 2090 2095

Ala Leu Asp Pro Pro Leu Phe Thr Arg Tyr Leu Arg Ile His Pro Thr

2100 2105 2110

Ser Trp Ala Gln His Ile Ala Leu Arg Leu Glu Val Leu Gly Cys Glu

2115 2120 2125

Ala Gln Asp Leu Tyr

2130

<210> 29

<211> 1466

<212> PRT

<213> 人工序列(Artificial Sequence)

<400> 29

Met Gln Leu Glu Leu Ser Thr Cys Val Phe Leu Cys Leu Leu Pro Leu

1 5 10 15

Gly Phe Ser Ala Ile Arg Arg Tyr Tyr Leu Gly Ala Val Glu Leu Ser

20 25 30

Trp Asp Tyr Arg Gln Ser Glu Leu Leu Arg Glu Leu His Val Asp Thr

35 40 45

Arg Phe Pro Ala Thr Ala Pro Gly Ala Leu Pro Leu Gly Pro Ser Val

50 55 60

Leu Tyr Lys Lys Thr Val Phe Val Glu Phe Thr Asp Gln Leu Phe Ser

65 70 75 80

Val Ala Arg Pro Arg Pro Pro Trp Met Gly Leu Leu Gly Pro Thr Ile

85 90 95

Gln Ala Glu Val Tyr Asp Thr Val Val Val Thr Leu Lys Asn Met Ala

100 105 110

Ser His Pro Val Ser Leu His Ala Val Gly Val Ser Phe Trp Lys Ser

115 120 125

Ser Glu Gly Ala Glu Tyr Glu Asp His Thr Ser Gln Arg Glu Lys Glu

130 135 140

Asp Asp Lys Val Leu Pro Gly Lys Ser Gln Thr Tyr Val Trp Gln Val

145 150 155 160

Leu Lys Glu Asn Gly Pro Thr Ala Ser Asp Pro Pro Cys Leu Thr Tyr

165 170 175

Ser Tyr Leu Ser His Val Asp Leu Val Lys Asp Leu Asn Ser Gly Leu

180 185 190

Ile Gly Ala Leu Leu Val Cys Arg Glu Gly Ser Leu Thr Arg Glu Arg

195 200 205

Thr Gln Asn Leu His Glu Phe Val Leu Leu Phe Ala Val Phe Asp Glu

210 215 220

Gly Lys Ser Trp His Ser Ala Arg Asn Asp Ser Trp Thr Arg Ala Met

225 230 235 240

Asp Pro Ala Pro Ala Arg Ala Gln Pro Ala Met His Thr Val Asn Gly

245 250 255

Tyr Val Asn Arg Ser Leu Pro Gly Leu Ile Gly Cys His Lys Lys Ser

260 265 270

Val Tyr Trp His Val Ile Gly Met Gly Thr Ser Pro Glu Val His Ser

275 280 285

Ile Phe Leu Glu Gly His Thr Phe Leu Val Arg His His Arg Gln Ala

290 295 300

Ser Leu Glu Ile Ser Pro Leu Thr Phe Leu Thr Ala Gln Thr Phe Leu

305 310 315 320

Met Asp Leu Gly Gln Phe Leu Leu Phe Cys His Ile Ser Ser His His

325 330 335

His Gly Gly Met Glu Ala His Val Arg Val Glu Ser Cys Ala Glu Glu

340 345 350

Pro Gln Leu Arg Arg Lys Ala Asp Glu Glu Glu Asp Tyr Asp Asp Asn

355 360 365

Leu Tyr Asp Ser Asp Met Asp Val Val Arg Leu Asp Gly Asp Asp Val

370 375 380

Ser Pro Phe Ile Gln Ile Arg Ser Val Ala Lys Lys His Pro Lys Thr

385 390 395 400

Trp Val His Tyr Ile Ser Ala Glu Glu Glu Asp Trp Asp Tyr Ala Pro

405 410 415

Ala Val Pro Ser Pro Ser Asp Arg Ser Tyr Lys Ser Leu Tyr Leu Asn

420 425 430

Ser Gly Pro Gln Arg Ile Gly Arg Lys Tyr Lys Lys Ala Arg Phe Val

435 440 445

Ala Tyr Thr Asp Val Thr Phe Lys Thr Arg Lys Ala Ile Pro Tyr Glu

450 455 460

Ser Gly Ile Leu Gly Pro Leu Leu Tyr Gly Glu Val Gly Asp Thr Leu

465 470 475 480

Leu Ile Ile Phe Lys Asn Lys Ala Ser Arg Pro Tyr Asn Ile Tyr Pro

485 490 495

His Gly Ile Thr Asp Val Ser Ala Leu His Pro Gly Arg Leu Leu Lys

500 505 510

Gly Trp Lys His Leu Lys Asp Met Pro Ile Leu Pro Gly Glu Thr Phe

515 520 525

Lys Tyr Lys Trp Thr Val Thr Val Glu Asp Gly Pro Thr Lys Ser Asp

530 535 540

Pro Arg Cys Leu Thr Arg Tyr Tyr Ser Ser Ser Ile Asn Leu Glu Lys

545 550 555 560

Asp Leu Ala Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys Tyr Lys Glu

565 570 575

Ser Val Asp Gln Arg Gly Asn Gln Met Met Ser Asp Lys Arg Asn Val

580 585 590

Ile Leu Phe Ser Val Phe Asp Glu Asn Gln Ser Trp Tyr Leu Ala Glu

595 600 605

Asn Ile Gln Arg Phe Leu Pro Asn Pro Asp Gly Leu Gln Pro Gln Asp

610 615 620

Pro Glu Phe Gln Ala Ser Asn Ile Met His Ser Ile Asn Gly Tyr Val

625 630 635 640

Phe Asp Ser Leu Gln Leu Ser Val Cys Leu His Glu Val Ala Tyr Trp

645 650 655

Tyr Ile Leu Ser Val Gly Ala Gln Thr Asp Phe Leu Ser Val Phe Phe

660 665 670

Ser Gly Tyr Thr Phe Lys His Lys Met Val Tyr Glu Asp Thr Leu Thr

675 680 685

Leu Phe Pro Phe Ser Gly Glu Thr Val Phe Met Ser Met Glu Asn Pro

690 695 700

Gly Leu Trp Val Leu Ala Cys His Asn Ser Asp Leu Arg Asn Arg Gly

705 710 715 720

Met Thr Ala Leu Leu Lys Val Tyr Ser Cys Asp Arg Asp Ile Gly Asp

725 730 735

Tyr Tyr Asp Asn Thr Tyr Glu Asp Ile Pro Gly Phe Leu Leu Ser Gly

740 745 750

Lys Asn Val Ile Glu Pro Arg Ser Phe Ser Gln Asn Ser Arg Pro Pro

755 760 765

Ser Ala Ser Ala Pro Lys Pro Pro Val Leu Arg His Gln Arg Asp Ile

770 775 780

Ser Leu Pro Thr Arg Gln Pro Glu Glu Asp Lys Met Asp Tyr Asp Asp

785 790 795 800

Ile Phe Ser Thr Glu Thr Lys Gly Glu Asp Phe Asp Ile Tyr Gly Glu

805 810 815

Asp Glu Asn Gln Asp Pro Arg Ser Phe Gln Lys Arg Thr Arg His Tyr

820 825 830

Phe Ile Ala Ala Val Glu Gln Leu Trp Asp Tyr Gly Met Ser Glu Ser

835 840 845

Pro Arg Ala Leu Arg Asn Arg Ala Gln Asn Gly Glu Val Pro Arg Phe

850 855 860

Lys Lys Val Val Phe Arg Glu Phe Ala Asp Gly Ser Phe Thr Gln Pro

865 870 875 880

Ser Tyr Arg Gly Glu Leu Asn Lys His Leu Gly Leu Leu Gly Pro Tyr

885 890 895

Ile Arg Ala Glu Val Glu Asp Asn Ile Met Val Thr Phe Lys Asn Gln

900 905 910

Ala Ser Arg Pro Tyr Ser Phe Tyr Ser Ser Leu Ile Ser Tyr Pro Asp

915 920 925

Asp Gln Glu Gln Gly Ala Glu Pro Arg His Asn Phe Val Gln Pro Asn

930 935 940

Glu Thr Arg Thr Tyr Phe Trp Lys Val Gln His His Met Ala Pro Thr

945 950 955 960

Glu Asp Glu Phe Asp Cys Lys Ala Trp Ala Tyr Phe Ser Asp Val Asp

965 970 975

Leu Glu Lys Asp Val His Ser Gly Leu Ile Gly Pro Leu Leu Ile Cys

980 985 990

Arg Ala Asn Thr Leu Asn Ala Ala His Gly Arg Gln Val Thr Val Gln

995 1000 1005

Glu Phe Ala Leu Phe Phe Thr Ile Phe Asp Glu Thr Lys Ser Trp Tyr

1010 1015 1020

Phe Thr Glu Asn Val Glu Arg Asn Cys Arg Ala Pro Cys His Leu Gln

1025 1030 1035 1040

Met Glu Asp Pro Thr Leu Lys Glu Asn Tyr Arg Phe His Ala Ile Asn

1045 1050 1055

Gly Tyr Val Met Asp Thr Leu Pro Gly Leu Val Met Ala Gln Asn Gln

1060 1065 1070

Arg Ile Arg Trp Tyr Leu Leu Ser Met Gly Ser Asn Glu Asn Ile His

1075 1080 1085

Ser Ile His Phe Ser Gly His Val Phe Ser Val Arg Lys Lys Glu Glu

1090 1095 1100

Tyr Lys Met Ala Val Tyr Asn Leu Tyr Pro Gly Val Phe Glu Thr Val

1105 1110 1115 1120

Glu Met Leu Pro Ser Lys Val Gly Ile Trp Arg Ile Glu Cys Leu Ile

1125 1130 1135

Gly Glu His Leu Gln Ala Gly Met Ser Thr Thr Phe Leu Val Tyr Ser

1140 1145 1150

Lys Glu Cys Gln Ala Pro Leu Gly Met Ala Ser Gly Arg Ile Arg Asp

1155 1160 1165

Phe Gln Ile Thr Ala Ser Gly Gln Tyr Gly Gln Trp Ala Pro Lys Leu

1170 1175 1180

Ala Arg Leu His Tyr Ser Gly Ser Ile Asn Ala Trp Ser Thr Lys Asp

1185 1190 1195 1200

Pro His Ser Trp Ile Lys Val Asp Leu Leu Ala Pro Met Ile Ile His

1205 1210 1215

Gly Ile Met Thr Gln Gly Ala Arg Gln Lys Phe Ser Ser Leu Tyr Ile

1220 1225 1230

Ser Gln Phe Ile Ile Met Tyr Ser Leu Asp Gly Arg Asn Trp Gln Ser

1235 1240 1245

Tyr Arg Gly Asn Ser Thr Gly Thr Leu Met Val Phe Phe Gly Asn Val

1250 1255 1260

Asp Ala Ser Gly Ile Lys His Asn Ile Phe Asn Pro Pro Ile Val Ala

1265 1270 1275 1280

Arg Tyr Ile Arg Leu His Pro Thr His Tyr Ser Ile Arg Ser Thr Leu

1285 1290 1295

Arg Met Glu Leu Met Gly Cys Asp Leu Asn Ser Cys Ser Met Pro Leu

1300 1305 1310

Gly Met Gln Asn Lys Ala Ile Ser Asp Ser Gln Ile Thr Ala Ser Ser

1315 1320 1325

His Leu Ser Asn Ile Phe Ala Thr Trp Ser Pro Ser Gln Ala Arg Leu

1330 1335 1340

His Leu Gln Gly Arg Thr Asn Ala Trp Arg Pro Arg Val Ser Ser Ala

1345 1350 1355 1360

Glu Glu Trp Leu Gln Val Asp Leu Gln Lys Thr Val Lys Val Thr Gly

1365 1370 1375

Ile Thr Thr Gln Gly Val Lys Ser Leu Leu Ser Ser Met Tyr Val Lys

1380 1385 1390

Glu Phe Leu Val Ser Ser Ser Gln Asp Gly Arg Arg Trp Thr Leu Phe

1395 1400 1405

Leu Gln Asp Gly His Thr Lys Val Phe Gln Gly Asn Gln Asp Ser Ser

1410 1415 1420

Thr Pro Val Val Asn Ala Leu Asp Pro Pro Leu Phe Thr Arg Tyr Leu

1425 1430 1435 1440

Arg Ile His Pro Thr Ser Trp Ala Gln His Ile Ala Leu Arg Leu Glu

1445 1450 1455

Val Leu Gly Cys Glu Ala Gln Asp Leu Tyr

1460 1465

Claims (9)

1.一种编码高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的核酸，其特征在于：

(1)hFⅧcDNA：核苷酸序列如SEQ ID NO:1-4任一所示；

或(2)BDD hFⅧ-SQ：核苷酸序列如SEQ ID NO:5-8任一所示；

或(3)BDD hFⅧ-N8：核苷酸序列如SEQ ID NO:9-12任一所示；

或(4)BDD hFⅧ-SC：核苷酸序列如SEQ ID NO:13-16任一所示；

或(5)BDD hFⅧ-△F：核苷酸序列如SEQ ID NO:17-20任一所示。

2.一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白，其特征在于：氨基酸序列如SEQ ID NO:21-29任一所示，该突变***于第710位的氨基酸为Ala而非野生型Ⅷ或Ⅷa的Gly。

3.编码如权利要求2中所述的突变蛋白的核酸，或与所述编码核酸长度相同且与所述编码核酸完全互补的核酸。

4.一种表达如权利要求2中所述的突变蛋白的载体。

5.一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白的制备方法，包括如下步骤：

以BDD-hFⅧ或全长FⅧ为模板，用PCR定点诱变法在野生型FⅧ中引入突变位点，将Gly710氨基酸残基替换成Ala，经转化后筛选出正确***Gly710Ala突变的BDD-hFⅧ或全长FⅧ真核表达质粒。

6.一种高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白编码核酸的应用，其特征在于：应用权利要求1或3所述的核酸于制备基因治疗药物，包括将其与启动子和终止序列连接，构建表达质粒、基因治疗病毒或非病毒载体，表达权利要求2中的突变蛋白。

7.一种如权利要求2所述的高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白的应用，其特征在于：应用于制备血友病的重组蛋白治疗药物。

8.一种如权利要求2所述的高活性的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala的突变蛋白的应用，其特征在于：应用于制备凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala突变体融合蛋白，并将所述融合蛋白用于制备血友病的重组蛋白治疗药物。

9.根据权利要求8所述的应用，其特征在于：所述融合蛋白为由人白蛋白、免疫球蛋白Fc、转铁蛋白或alpha 1抗胰蛋白酶中的一种与所述的凝血因子Ⅷ或Ⅷa多肽变体Gly710Ala突变体的突变蛋白融合而得。

Images