CN114146676A - Method for controlling sequential mixed reaction of reagents by centrifugal force - Google Patents
Method for controlling sequential mixed reaction of reagents by centrifugal force Download PDFInfo
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- CN114146676A CN114146676A CN202111500270.5A CN202111500270A CN114146676A CN 114146676 A CN114146676 A CN 114146676A CN 202111500270 A CN202111500270 A CN 202111500270A CN 114146676 A CN114146676 A CN 114146676A
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 149
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 140
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000002156 mixing Methods 0.000 claims abstract description 37
- 238000007789 sealing Methods 0.000 claims description 11
- 238000002955 isolation Methods 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 4
- 239000002904 solvent Substances 0.000 abstract 2
- 238000010586 diagram Methods 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007810 chemical reaction solvent Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/28—Moving reactors, e.g. rotary drums
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Abstract
The invention relates to a method for controlling sequential mixing reaction of reagents by centrifugal force, which comprises adding sample solvent into a reaction vessel, sequentially arranging at least one reagent chamber above the sample solvent, after the reaction reagent is added into the reagent chamber, the reaction container is arranged on the driving device and is driven to do circular motion by the rotating shaft arranged in the driving device, and the included angle between the reaction vessel and the rotating shaft is arranged in an oblique angle, the reaction vessel is obliquely arranged in the driving device, thereby changing the flow direction of the sample reagent in the reaction container under the action of centripetal force, mixing the sample reagent with the reaction reagent according to the arrangement sequence of the reagent chambers, thereby effectively improving the mixing reaction efficiency of the chemical reagent, automatically controlling the sequential mixing of the sample reagent and the reaction reagent and bringing great convenience to the use of people.
Description
Technical Field
The invention relates to the technical field of tests, in particular to a method for controlling sequential mixing reaction of reagents by centrifugal force.
Background
Chemical reagents are relatively standard substances for chemical research and component analysis, are important conditions for technological progress, are widely used for synthesis, separation, qualitative and quantitative analysis of substances, can be said to be eyes of chemists, and are not separated from chemical reagents in daily work of factories, schools, hospitals and research institutes.
In the prior art, in the mixing reaction of tests or tests in various fields, a sample reagent and a reaction reagent are generally mixed by manually stirring or shaking a reagent tube by a driving device, but the sequential mixing of the reaction reagent and the sample reagent in the mixing reaction method needs to be manually controlled, which not only increases the labor capacity of people, but also reduces the mixing reaction efficiency.
Disclosure of Invention
In order to solve the problems, the invention provides a method for controlling the sequential mixing reaction of reagents by centrifugal force, which can automatically control the sequential mixing of sample reagents and reaction reagents and improve the test efficiency.
In order to achieve the above purpose, the method for controlling the sequential mixing reaction of reagents by centrifugal force according to the present invention mainly comprises the following steps:
a. respectively sampling a sample reagent and a reaction reagent, and carrying out isolation treatment on the sample reagent and the reaction reagent;
b. sequentially arranging a reaction reagent and a sample reagent in a reaction container according to the sequence of mixing reaction;
c. and applying a force to the reaction vessel to move the sample reagent and the reaction reagent in the direction of the set mixed reaction sequence. The method moves the reaction container by applying force to the reaction container, drives the position of the sample reagent added into the reaction container to shift, and mixes and reacts with the reaction reagent according to the mixing reaction sequence set in the step b.
The further proposal is that the force given in the step c is specifically as follows: and applying a driving force to the reaction container to make the reaction container perform circular motion. The method enables the reaction vessel to move within a determined range to complete sequential mixing reaction by applying a driving force of circular motion to the reaction vessel.
The further scheme is that the step b is sequentially provided with the following specific steps: the reaction reagent and the sample reagent are arranged in the reaction vessel in the order of distance from the rotation axis of the circular motion. The method comprises the steps of arranging reaction reagents and sample reagents on different positions of a reaction container according to a sequential mixing sequence, and controlling the height and duration of a mixed liquid through centrifugal force or other driving forces, so that sufficient reaction time is reserved between the reaction reagents and the sample reagents.
The further scheme is that the isolation treatment in the step a specifically comprises the following steps: the method is characterized in that at least one reagent chamber is arranged in a reaction container, a sample reagent is added into the reaction container, a reaction reagent is added into the reagent chamber arranged in the reaction container, a slot for the reaction reagent to flow out is formed in the reagent chamber, and different reaction reagents are arranged in the reagent chamber from far to near from a rotating shaft which moves circumferentially according to a mixing sequence. In the method, when the reaction container makes circular motion, the reagent in the reaction container deviates towards the outer side of the reaction container under the action of centripetal force, so that the outflow of the reaction reagent is controlled through the open slot formed in the reagent chamber.
In a further scheme, a sealing cover is arranged at the top of the reaction container, and a drying reagent is arranged on the inner side of the sealing cover. The method arranges the dried reagent and the sample reagent at different heights in the reaction container, thereby achieving the purpose of mutually isolating the reagents in the reaction container while not arranging a reagent chamber.
In a further embodiment, the reaction vessel in step c is arranged on a driving device for providing a driving force for the circular motion to the reaction vessel, and the bottom of the reaction vessel is closer to the rotating shaft of the circular motion relative to the top of the reaction vessel. The method adjusts the gradient of the liquid level of the sample reagent in the reaction container through the reaction container which is arranged obliquely according to the rotating speed of the rotating shaft, thereby controlling the mixing of the sample reagent and the reaction solvent.
The invention relates to a method for controlling sequential mixing reaction of reagents by centrifugal force, which is characterized in that a reaction container is obliquely arranged in a driving device, a rotating shaft arranged in the driving device drives the reaction container to do non-uniform circular motion, sample reagents added in the reaction container are mixed with reaction reagents added in reagent chambers along the inner wall of the reaction container in a sequence from far to near from the rotating shaft under the action of centripetal force, and distances for the reaction reagents to react with the sample reagents are reserved among different reagent chambers, so that the aims of effectively improving the mixing reaction efficiency of chemical reagents and automatically controlling the sequential mixing of the sample reagents and the reaction reagents are fulfilled, and great convenience is brought to the use of people.
Drawings
FIG. 1 is a schematic diagram of an apparatus using the method described in example 1.
FIG. 2 is a schematic view of an apparatus for preliminary mixing of a sample reagent with a first reaction reagent.
FIG. 3 is a schematic view of an apparatus for further mixing of a sample reagent with a second reaction reagent
FIG. 4 is a schematic view of the apparatus in the final state of the sequential mixing reaction of reagents.
FIG. 5 is a schematic diagram of the mixing process in step E of the method described using example 2.
FIG. 6 is a schematic diagram of the mixing process in step F of the method described using example 2.
FIG. 7 is a schematic diagram of the mixing process in step G of the method described using example 2.
Detailed Description
To further illustrate the technical means and effects of the present invention adopted to achieve the predetermined objects, the following detailed description of the embodiments, structures, features and effects according to the present invention will be made with reference to the accompanying drawings and preferred embodiments.
Example 1.
As shown in fig. 1, the method for controlling sequential mixing reaction of reagents by centrifugal force described in this embodiment mainly includes the following steps:
A. respectively sampling a sample reagent 2, a first reaction reagent 10 and a second reaction reagent 3;
B. a first reaction reagent 10 and a second reaction reagent 3 are added into the first reagent chamber 4 and the second reagent chamber 9 along the open grooves formed on the first reagent chamber 4 and the second reagent chamber 9, and a sample reagent 2 is added into the reaction container 1 along the bottle opening of the reaction container 1;
C. a sealing cover 7 is covered at the bottleneck of the reaction vessel 1, and a drying reagent 8 is attached to the inner side of the sealing cover 7;
D. a reaction vessel fixing groove which is arranged in an inverted V shape is formed in the driving device 5, and the reaction vessel 1 is fixedly arranged in the reaction vessel fixing groove to obtain a graph 1;
E. the initial rotating speed of the rotating shaft 6 drives the reaction container 1 to do circular motion, so that the first reaction reagent 10 in the first reagent chamber 4 flows towards the included angle between the reaction container 1 and the sealing cover 7 above the first reagent chamber 4 under the action of centripetal force, and a graph 2 is obtained;
F. the speed of the reaction vessel 1 is increased by the accelerated rotation of the rotating shaft 6, so that the sample reagent 2 is mixed with the first reaction reagent 10 at the corner between the reaction vessel 1 and the sealing cover 7 under the action of centripetal force, and a graph 3 is obtained; at this time, the rotation speed may be maintained for a period of time as required to allow the sample reagent 2 and the first reaction reagent 10 to be sufficiently mixed and reacted;
G. and (3) accelerating the rotation of the rotating shaft 6 to the maximum speed, further increasing the speed of the reaction container 1, further mixing and reacting the dried reagent 8 with the mixed reagent obtained in the step (F), and simultaneously adding the second reaction reagent 3 into the second reagent chamber 9 to react with the mixed reagent under the action of centripetal force, thereby obtaining the graph 4.
Example 2.
The method for controlling the sequential mixing reaction of reagents by centrifugal force described in this example mainly comprises the following steps:
A. respectively sampling a sample reagent 2, a first reaction reagent 10 and a second reaction reagent 3;
B. a first reaction reagent 10 and a second reaction reagent 3 are added into the first reagent chamber 4 and the second reagent chamber 9 along the open grooves formed on the first reagent chamber 4 and the second reagent chamber 9, and a sample reagent 2 is added into the reaction container 1 along the bottle opening of the reaction container 1;
C. a sealing cover 7 is covered at the bottleneck of the reaction container 1, a drying reagent 8 is attached to the inner side of the sealing cover 7, and the drying reagent 8 is arranged on one side far away from the rotating shaft;
D. a reaction vessel fixing groove which is arranged in an inverted V shape is formed in the driving device 5, and the reaction vessel 1 is fixedly arranged in the reaction vessel fixing groove;
E. the initial rotating speed of the rotating shaft 6 drives the reaction container 1 to do circular motion, so that the first reaction reagent 10 in the first reagent chamber 4 flows towards an included angle between the reaction container 1 and the sealing cover 7 above the first reagent chamber 4 under the action of centripetal force, and meanwhile, the dried reagent 8 is further mixed and reacted with the reagents to obtain a graph 5;
F. the speed of the reaction container 1 is increased through the accelerated rotation of the rotating shaft 6, so that the sample reagent 2 reacts with the mixed reagent above the first reagent chamber 4 under the action of centripetal force, and a graph 6 is obtained, and at the moment, the rotating speed can be kept for a period of time as required so that the sample reagent 2 and the mixed reagent above the first reagent chamber 4 are fully mixed and react;
G. the speed of the reaction container 1 is further increased by the accelerated rotation of the rotating shaft 6 to the maximum speed, so that the second reaction reagent 3 added into the second reagent chamber 9 is mixed and reacted with the mixed reagent obtained in the step F under the action of centripetal force, and then fig. 7 is obtained.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (6)
1. A method for controlling sequential mixing reactions of reagents by centrifugal force, comprising the steps of:
a. respectively sampling a sample reagent and a reaction reagent, and carrying out isolation treatment on the sample reagent and the reaction reagent;
b. sequentially arranging a reaction reagent and a sample reagent in a reaction container according to the sequence of mixing reaction;
c. and applying a force to the reaction vessel to move the sample reagent and the reaction reagent in a direction along the set mixed reaction sequence.
2. The method for controlling the sequential mixing reaction of reagents by centrifugal force according to claim 1, wherein the force applied in step c is specifically: and applying a driving force to the reaction container to make the reaction container perform circular motion.
3. The method for controlling the sequential mixing reaction of reagents by centrifugal force according to claim 1 or 2, wherein the step b comprises the following steps: the reaction reagent and the sample reagent are arranged in the reaction vessel in the order of distance from the rotation axis of the circular motion.
4. The method for controlling the sequential mixing reaction of reagents by centrifugal force according to claim 3, wherein the isolation process performed in step a is specifically: the method is characterized in that at least one reagent chamber is arranged in a reaction container, a sample reagent is added into the reaction container, a reaction reagent is added into the reagent chamber arranged in the reaction container, a slot for the reaction reagent to flow out is formed in the reagent chamber, and different reaction reagents are arranged in the reagent chamber from far to near from a rotating shaft which moves circumferentially according to a mixing sequence.
5. The method of claim 4, wherein a sealing cover is disposed on the top of the reaction vessel, and the dried reagent is disposed inside the sealing cover.
6. A method for controlling the sequential mixing reaction of reagents by centrifugal force as claimed in claim 5, wherein the reaction vessel in step c is mounted on a driving means for providing a driving force for the circular motion to the reaction vessel, and is located at the bottom of the reaction vessel closer to the rotating shaft of the circular motion than to the top of the reaction vessel.
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| CN202111500270.5A CN114146676B (en) | 2021-12-09 | 2021-12-09 | Method for controlling sequential mixing reaction of reagents by centrifugal force |
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| CN202111500270.5A CN114146676B (en) | 2021-12-09 | 2021-12-09 | Method for controlling sequential mixing reaction of reagents by centrifugal force |
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| CN114146676B CN114146676B (en) | 2023-12-15 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110045102A (en) * | 2019-04-08 | 2019-07-23 | 深圳市刚竹医疗科技有限公司 | Reagent sequence loading device, centrifugal microfluidic control device and analysis system |
| CN110142066A (en) * | 2019-04-25 | 2019-08-20 | 深圳市刚竹医疗科技有限公司 | Micro-fluidic chip and analysis system |
| CN110180610A (en) * | 2019-06-19 | 2019-08-30 | 深圳市刚竹医疗科技有限公司 | Reagent sequence loading method, structure and micro fluidic device |
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- 2021-12-09 CN CN202111500270.5A patent/CN114146676B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110045102A (en) * | 2019-04-08 | 2019-07-23 | 深圳市刚竹医疗科技有限公司 | Reagent sequence loading device, centrifugal microfluidic control device and analysis system |
| CN110142066A (en) * | 2019-04-25 | 2019-08-20 | 深圳市刚竹医疗科技有限公司 | Micro-fluidic chip and analysis system |
| CN110180610A (en) * | 2019-06-19 | 2019-08-30 | 深圳市刚竹医疗科技有限公司 | Reagent sequence loading method, structure and micro fluidic device |
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