CN104353509B - Many reagent color comparison tube for portable colorimetric device - Google Patents

Many reagent color comparison tube for portable colorimetric device Download PDF

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Publication number
CN104353509B
CN104353509B CN201410635755.9A CN201410635755A CN104353509B CN 104353509 B CN104353509 B CN 104353509B CN 201410635755 A CN201410635755 A CN 201410635755A CN 104353509 B CN104353509 B CN 104353509B
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reagent
steel ball
color comparison
interior pipe
colorimetric device
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CN201410635755.9A
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CN104353509A (en
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邹炳德
杨中柱
谢远敏
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NINGBO MEIKANG SHENGDE BIOTECHNOLOGY CO Ltd
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NINGBO MEIKANG SHENGDE BIOTECHNOLOGY CO Ltd
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Publication of CN104353509A publication Critical patent/CN104353509A/en
Priority to PCT/CN2015/080770 priority patent/WO2016074474A1/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

A kind of many reagent color comparison tube for portable colorimetric device, it includes the outer tube (1) for depositing the first reagent (1.1) and/or detected sample and for depositing the interior pipe (2) of other reagent at least one, outer tube (1) upper end is provided with mean for the opening that interior pipe (2) inserts, first reagent (1.1) is liquid, and other reagent are solid, shaped structure;It also includes the steel ball (8) for stirring mix reagent, steel ball (8) is positioned at pipe (2), the first position-limit mechanism for other reagent and steel ball being left in interior pipe (2) it is provided with in interior pipe (2), steel ball (8) is positioned at below other reagent, and interior pipe (2) inwall upper end is provided with and makes its material jacking mechanism fallen in the first reagent (1.1) for steel ball (8) and other reagent being ejected。

Description

Many reagent color comparison tube for portable colorimetric device
Technical field
The present invention relates to sample detection field, be especially a kind of many reagent color comparison tube for portable colorimetric device。
Background technology
At present, color comparison tube is applied to sample chemical analysis detection, by instrument, reacted color comparison tube is carried out photometric detection, so that it is determined that the chemical composition of sample, color comparison tube is widely used in the detection field of environmental protection tests (as water quality of river detects), food quality detection, medical treatment detection or other industry, portable color comparison tube small volume, can be used for going out to carry, on-the-spot sample is detected, there is real-time, convenience。
Such as in many medical tests, need to mix sample to be measured with first liquid, it is easy to the interfering components in removal sample, starts the first chemical reaction or biological respinse, when sample to be detected mixes after certain time with first liquid, it is added to second liquid, start the second chemical reaction or biological respinse, if sample component to be detected is more complicated, even need to add the 3rd liquid or the 4th liquid, to start the 3rd reaction or the 4th reaction, whole process passes through its chemical reaction of photometric measurement。Such as, glycolated hemoglobin composition in test blood, need to mix the molten sample crossing blood with R1 (first liquid), destroy the composition being likely to impact test in sample, it is subsequently adding R2 (second liquid) to react again, detects finally by photometric detection device。
Photometric measurement for basic automatization, the automatic analyzer having various complexity measures, instrument to complete the application of sample of plurality of reagents, including complicated sample adding system, stirring system, purging system, heating system, cause whole instrument quite huge, complicated, price costly, and only has large hospital to be subject to special door start-up to operate;At present, existing one manually completes photometric measurement, application of sample, stirring semi-automatic biochemical analyzer, its relative volume is less, and existing semi-automatic biochemistry analyzer includes mixing container。
Color comparison tube is used for depositing and mixing sample to be detected and liquid in biochemical analysis instrument apparatus, color comparison tube includes for depositing first liquid, steel ball and the first mixing container with detection sample, for depositing the second mixing container of second liquid, when detection, mix container cover by first to take off, mix container by second and insert the first mixing upper vessel portion, and second the upper and lower of mixing container be respectively equipped with and can mix the sealing-plug that slide up and down of container inner wall second, when second liquid is mixed by needs with first liquid, the sealing-plug of the second mixing upper vessel portion is enforced by the push rod in biochemistry analyzer, thus driving the sealing-plug of second liquid and the second mixing lower vessel portion to move downward, when the sealing-plug of bottom departs from the second mixing container, second liquid mixes internal tank drippage due to gravity from second, and mix with first liquid, again through be previously positioned in the first mixing container steel ball and coordinate be independently arranged in biochemistry analyzer the dynamic first mixing container of magnetic agitation dribbling in steel ball rotate, thus mixed solution is stirred, make mixed solution react completely and stir, finally carry out photometric detection, above prior art comes with some shortcomings part:
1) for needing strict control with the consumption and ratio of the aitiogenic first liquid of sample and second liquid, and above by promoting sealing-plug thus realizing the mode that first liquid mixes with second liquid, second liquid is made easily to remain on inwall or the sealing-plug of the second mixing container, cause that the consumption participating in the second liquid of reaction is inaccurate, thus affecting the reaction of first liquid and second liquid, the accuracy ultimately resulting in photometric detection is not high, mistaken diagnosis or wrong report easily occurs on medical treatment detects;
2) existing color comparison tube is only only applicable to the mixing of two kinds of liquid reagents, it is impossible to carry out more than three kinds reagent tests;
3) owing to steel ball is to be encapsulated in the liquid in the first mixing container in advance, and steel ball is before being immersed in liquid, need the surface to steel ball to carry out special handling, and steel ball long period of soaking is likely to change so that the composition of liquid in liquid, and then be likely to affect the result of subsequent reactions。
Summary of the invention
The technical problem to be solved: provide a kind of photometric detection accuracy higher, suitable in two or more reaction reagent and the many reagent color comparison tube for portable colorimetric device not affecting subsequent reactions。
In order to solve above-mentioned technical problem, the present invention is achieved by the following technical solutions: a kind of many reagent color comparison tube for portable colorimetric device, it includes the outer tube for depositing the first reagent and detected sample and for depositing the interior pipe of other reagent at least one, outer tube upper end is provided with mean for the opening that interior pipe inserts, and other reagent are solid, shaped structure;It also includes the steel ball for stirring mix reagent, steel ball is positioned at pipe, the first position-limit mechanism for leaving in interior pipe by other reagent and steel ball it is provided with in interior pipe, steel ball is positioned at below other reagent, and inner tube wall upper end is provided with and makes its material jacking mechanism fallen in the first reagent for steel ball and other reagent being ejected。
Adopt said structure, advantage for present invention is: the one of which of existing first reagent or detected sample is liquid, the sample of liquid or the mixed solution of the first reagent and sample can be simply placed in outer tube, the present invention changes prior art and adopts the traditional view of liquid reagent and reacted liquid reagent, adopt solid state reagents to fall into liquid reagent and mix post-reacted mode, the solid state reagents participating in reaction is made to mix with liquid reagent completely and fully react, do not have prior art, because liquid reagent remains in, inner tube wall causing, final photometric detection is inaccurate, and then ensure that the accuracy of experiment;Steel ball is left in interior pipe, owing to other reagent in interior pipe are solid structure, make steel ball without influence on the chemical composition of other reagent, thus without influence on follow-up chemical reaction, and owing to steel ball is positioned at below other reagent, when needs stir, by material jacking mechanism, steel ball is first headed in the first reagent of outer tube and the mixed solution of sample, coordinating stirring mixed solution by prior art steel ball with the magnetic agitation dish in biochemistry analyzer, material jacking mechanism is positioned at biochemistry analyzer。
As preferably, described position-limit mechanism refers to the feed pipe being matched with in interior pipe that slides axially, and described feed pipe lower end sidewall is axially arranged with opening along feed pipe, is provided with the cavity for depositing other reagent in opening, described first position-limit mechanism is positioned at opening, and material jacking mechanism is positioned at above feed pipe。
As preferably, described first position-limit mechanism includes open bottom and is provided with at least two radial grooves for placing steel ball and other reagent along feed pipe length direction。
As preferably, other reagent described are chondritic, and described radial groove is arc groove, and the diameter of described steel ball and other reagent is more than the distance between open bottom to inner tube wall。
The present invention adopts feed pipe to be slidably fitted in inner tube wall, and feed pipe lower end sidewall is provided with opening, is provided with number and solid state reagents and steel ball radial groove one to one in opening, and radial groove is used for placement spacing solid state reagents and steel ball;Described solid state reagents is chondritic, radial groove is arc groove, other reagent of steel ball and chondritic are positioned at arc groove, and owing to other reagent of chondritic and the diameter of steel ball are more than the distance between open bottom to inner tube wall, other reagent and the steel ball that make chondritic are limited in the opening of feed pipe by the clamping of inner tube wall and arc groove, when other reagent and steel ball are added in the first reagent by needs, feed pipe is pushed down on by material jacking mechanism, when the opening of feed pipe side wall lower ends departs from inner tube wall, other reagent of chondritic and steel ball freely fall into the first reagent owing to losing the clamping of inner tube wall in arc groove, finally mixing container is put into biochemical analysis instrument apparatus, by the magnetic agitation dish in steel ball and biochemistry analyzer, mixed solution is stirred, it is made fully to react, finally carry out photometric detection, structure is simpler。
As preferably, the number of described arc groove is four, and the number of other reagent is three, and steel ball is positioned at the arc groove of bottommost。
As preferably, described three other reagent are the second reagent。
As preferably, described three other reagent respectively the second reagent and the 3rd reagent, described second reagent is between steel ball and the 3rd reagent。
Existing color comparison tube is only only applicable to the mixing of two kinds of liquid, and the present invention adopts the mode of solid reagent and liquid reagent, make in feed pipe, to deposit two or more other reagent participating in reaction, feed pipe is progressively promoted by material jacking mechanism, other reagent needing reaction make it fall into successively and in the first reagent, participates in reaction, present configuration is applicable to the reagent that three kinds or more is reacted with detected sample, realize many reagent detection of the small-sized spectrophotometric sensing device of prior art, the scope of application is wider, without changing interior pipe successively, and simple to operate。
As preferably, described feed pipe upper end outer wall is provided with at least one annular seal groove, is arranged with sealing ring in annular seal groove, and sealing is better, and makes to have between feed pipe and interior pipe certain frictional force so that feed pipe will not free landing in interior pipe。
As preferably, said inner tube lower ending opening is sliding combined with sealing-plug。
Accompanying drawing explanation
Fig. 1 is the present invention detonation configuration schematic diagram for many reagent color comparison tube of portable colorimetric device。
Fig. 2 is the present invention structural representation for many reagent color comparison tube of portable colorimetric device。
Fig. 3 be the present invention for second reagent to be added of many reagent color comparison tube of portable colorimetric device time status architecture schematic diagram。
Fig. 4 be the present invention for three reagent to be added of many reagent color comparison tube of portable colorimetric device time state machine schematic diagram。
Fig. 5 is the present invention side elevational cross-section structural representation for many reagent color comparison tube of portable colorimetric device。As shown in the figure: 1-outer tube, 1.1-the first reagent, pipe, 2.1-the second reagent, 2.2-the 3rd reagent, 3-sealing-plug, 4-feed pipe, 4.1-opening, 5-arc groove, 6-annular seal groove, 7-sealing ring, 8-steel ball in 2-。
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is described further。
In the present description, it will be appreciated that, term " on ", D score, " interior ", the indicating position such as " outward " or position relationship be based on the position relationship described in accompanying drawing, be for only for ease of the description present invention or simplify and describe, rather than the specific orientation that instruction must have。
As shown in Figure 1, Figure 2, shown in Fig. 3, Fig. 4, Fig. 5, a kind of many reagent color comparison tube for portable colorimetric device, it includes the outer tube 1 for depositing the first reagent 1.1 and detected sample and for depositing the interior pipe 2 of other reagent at least one, outer tube 1 upper end is provided with mean for the opening that interior pipe 2 inserts, and other reagent are solid, shaped structure;It also includes the steel ball 8 for stirring mix reagent, steel ball 8 is positioned at pipe 2, the first position-limit mechanism for leaving in interior pipe 2 by other reagent and steel ball 8 it is provided with in interior pipe 2, steel ball 8 is positioned at below other reagent, and interior pipe 2 inwall upper end is provided with and makes its material jacking mechanism fallen in the first reagent 1.1 for being ejected by other reagent of steel ball 8。
The present invention changes prior art and adopts the traditional view of liquid reagent and reacted liquid reagent, adopt solid state reagents to fall into liquid reagent and mix post-reacted mode, the solid state reagents participating in reaction is made to mix with liquid reagent completely and fully react, do not have prior art, because liquid reagent remains in, inner tube wall causing, final photometric detection is inaccurate, and then ensure that the accuracy of experiment;Steel ball 8 is left in interior pipe 2, owing to other reagent in interior pipe 2 are solid structure, make steel ball 8 without influence on the chemical composition of other reagent, thus without influence on follow-up chemical reaction, and owing to steel ball 8 is positioned at below other reagent, when needs stir, by material jacking mechanism, steel ball 8 is first headed in the first reagent 1.1 of outer tube 1 and the mixed solution of sample, coordinate stirring mixed solution with magnetic agitation dish by prior art steel ball 8。
Described position-limit mechanism refers to the feed pipe 4 being matched with in interior pipe 2 that slides axially, described feed pipe 4 lower end sidewall is axially arranged with opening 4.1 along feed pipe 4, the cavity for depositing other reagent it is provided with in opening 4.1, described first position-limit mechanism is positioned at opening 4.1, and material jacking mechanism is positioned at above feed pipe 4;Described first position-limit mechanism includes being provided with at least one radial groove for placing other reagent along feed pipe 4 length direction bottom opening 4.1;Other reagent described are chondritic, and described radial groove is arc groove 5, and the diameter of other reagent described is more than the distance arrived bottom opening 4.1 between interior pipe 2 inwall。
The present invention adopts feed pipe 4 to be slidably fitted in interior pipe 2 inwall, and feed pipe 4 lower end sidewall is provided with opening 4.1, is provided with number and solid state reagents and steel ball 8 radial groove one to one in opening 4.1, and radial groove is used for placement spacing solid state reagents and steel ball 8;Described solid state reagents is chondritic, radial groove is arc groove 5, other reagent of chondritic and steel ball are positioned at arc groove 5, and owing to other reagent of chondritic and the diameter of steel ball 8 are more than the distance arrived bottom opening 4.1 between interior pipe 2 inwall, other reagent and the steel ball 8 that make chondritic are limited in the opening 4.1 of feed pipe 4 by the clamping of interior pipe 2 inwall and arc groove 5, when steel ball 8 and other reagent are added in the first reagent 1.1 by needs, feed pipe 4 is pushed down on by material jacking mechanism, when the opening 4.1 of feed pipe 4 side wall lower ends departs from interior pipe 2 inwall, other reagent of chondritic and steel ball freely fall into the first reagent 1.1 owing to losing the clamping of interior pipe 2 inwall in arc groove 5, finally mixing container is put into biochemical analysis instrument apparatus, by the magnetic agitation dish in steel ball 8 and biochemistry analyzer, mixed solution is stirred, it is made fully to react, finally carry out photometric detection, structure is simpler。
The present invention adopts feed pipe 4 to carry out spacing to other spherical reagent, can also by other means other reagent of solid-state be carried out spacing, as interior pipe 2 inwall is provided with spacing ring along axis, other spherical reagent are positioned at above spacing ring, spacing ring is flexible material, by material jacking mechanism, other reagent are ejected action, other reagent are headed in the first reagent 1.1 from cooperation really up to the mark above spacing ring。
Described arc groove 5 is four for number, and the number of other reagent is three, and steel ball 8 is positioned at the arc groove 5 of bottommost。
Described three other reagent are the second reagent 2.1。
Described three other reagent respectively the second reagent 2.1 and the 3rd reagent 3.1, described second reagent 2.1 is between steel ball and the 3rd reagent 2.2。
The number of arc groove can increase along with the length of reagent, such that it is able to increase the quantity of other reagent, existing color comparison tube is only only applicable to the mixing of two kinds of liquid, and the present invention adopts the mode of solid reagent and liquid reagent, make in feed pipe 4, to deposit two or more other reagent participating in reaction, feed pipe 4 is progressively promoted by material jacking mechanism, other reagent needing reaction make it fall into successively and in the first reagent .1.1, participates in reaction, present configuration is applicable to the reagent that three kinds or more is reacted with detected sample, the scope of application is wider, without changing interior pipe 2 successively, and it is simple to operate;Meanwhile, other reagent can also be same reagent so that color comparison tube of the present invention also can complete prior art needs two kinds and detected sample response situation;Additionally the present invention only enumerates the second reagent and the 3rd reagent, it is also possible to have other reagent such as the 4th reagent and the 5th reagent。
The concrete operation step that the present invention adds reagent is as follows:
1. take off outer tube 1 to cover, interior pipe 2 is inserted outer tube 1 top;
2. the color comparison tube assembled is put into biochemical analysis, promote feed pipe 4 certain distance by the material jacking mechanism in biochemistry analyzer so that the mixed solution of the first reagent 1.1 and sample fallen into by the steel ball 8 being positioned at feed pipe 4 foot;
3. the dynamic steel ball 8 of the magnetic vibration dribbling in biochemistry analyzer rotates in outer tube 1, is used for stirring the first reagent 1.1 and sample and making it fully react;
4. after the first reagent 1.1 and example reaction are complete, continue to press on feed pipe 4, other reagent in feed pipe 4 are gradually added in the mixed solution of the first reagent 1.1 and sample;
5. 3. and 4. repeat the above steps operates so that chemical reaction participated in successively by other reagent being gradually added in mixed solution;
6. photometric detection is carried out。
Described feed pipe 4 upper end outer wall is provided with at least one annular seal groove 6, is arranged with sealing ring 7 in annular seal groove 6, and sealing is better, and makes to have between feed pipe 4 and interior pipe 2 certain frictional force so that feed pipe 4 will not free landing in interior pipe 2。
Said inner tube 2 lower ending opening is sliding combined with sealing-plug 3。
The solidification processing mode of other reagent that the present invention adopts can adopt liquid nitrogen freezing or other existing can make the solidified mode of liquid reagent。
Additionally, the portable many reagent color comparison tube of the present invention can also be applied to other detection industry, such as food quality detection or environmental protection water quality of river detection etc., operating procedure duplicates with aforesaid operations step;If when being simply placed in detection sample (such as the detection water quality of river) of liquid in outer tube, it is possible to will only be placed with river sample in outer tube, and reagent is all put in interior pipe, react one by one finally by aforesaid operations step and water quality is detected, detection multiformity, the scope of application is big, more convenient。
Above the present invention and embodiment thereof being described, this description does not have restricted, and shown in accompanying drawing is also one of embodiments of the present invention, and actual structure is not limited thereto。If generally speaking those of ordinary skill in the art is enlightened by it, when without departing from the invention objective, design the frame mode similar to this technical scheme and embodiment without creationary, protection scope of the present invention all should be belonged to。

Claims (9)

1. the many reagent color comparison tube for portable colorimetric device, it is characterized in that: it includes the outer tube (1) for depositing the first reagent (1.1) and/or detected sample and for depositing the interior pipe (2) of other reagent at least one, outer tube (1) upper end is provided with mean for the opening that interior pipe (2) inserts, first reagent (1.1) is liquid, and other reagent are solid, shaped structure;It also includes the steel ball (8) for stirring mix reagent, steel ball (8) is positioned at pipe (2), the first position-limit mechanism for other reagent and steel ball being left in interior pipe (2) it is provided with in interior pipe (2), steel ball (8) is positioned at below other reagent, and interior pipe (2) inwall upper end is provided with and makes its material jacking mechanism fallen in the first reagent (1.1) for steel ball (8) and other reagent being ejected。
2. the many reagent color comparison tube for portable colorimetric device according to claim 1, it is characterized in that: described position-limit mechanism refers to the feed pipe (4) being matched with in interior pipe (2) that slides axially, described feed pipe (4) lower end sidewall is axially arranged with opening (4.1) along feed pipe (4), the cavity for depositing other reagent it is provided with in opening (4.1), described first position-limit mechanism is positioned at opening (4.1), and material jacking mechanism is positioned at feed pipe (4) top。
3. the many reagent color comparison tube for portable colorimetric device according to claim 2, it is characterised in that: described first position-limit mechanism includes opening (4.1) bottom and is provided with at least two radial grooves for placing steel ball (8) and other reagent along feed pipe (4) length direction。
4. the many reagent color comparison tube for portable colorimetric device according to claim 3, it is characterized in that: other reagent described are chondritic, described radial groove is arc groove (5), and the diameter of described steel ball (8) and other reagent is more than the distance between opening (4.1) bottom to interior pipe (2) inwall。
5. the many reagent color comparison tube for portable colorimetric device according to claim 4, it is characterized in that: described arc groove (5) is four for number, the number of other reagent is three, and steel ball (8) is positioned at the arc groove (5) of bottommost。
6. the many reagent color comparison tube for portable colorimetric device according to claim 5, it is characterised in that: described three other reagent are the second reagent (2.1)。
7. the many reagent color comparison tube for portable colorimetric device according to claim 5, it is characterized in that: described three other reagent respectively the second reagent (2.1) and the 3rd reagent (2.2), described second reagent (2.1) is positioned between steel ball and the 3rd reagent (2.2)。
8. the many reagent color comparison tube for portable colorimetric device according to claim 2, it is characterized in that: described feed pipe (4) upper end outer wall is provided with at least one annular seal groove (6), is arranged with sealing ring (7) in annular seal groove (6)。
9. the many reagent color comparison tube for portable colorimetric device according to claim 1, it is characterised in that: said inner tube (2) lower ending opening is sliding combined with sealing-plug (3)。
CN201410635755.9A 2014-11-12 2014-11-12 Many reagent color comparison tube for portable colorimetric device Active CN104353509B (en)

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CN201410635755.9A CN104353509B (en) 2014-11-12 2014-11-12 Many reagent color comparison tube for portable colorimetric device
PCT/CN2015/080770 WO2016074474A1 (en) 2014-11-12 2015-06-04 Multi-reagent colorimetric tube for portable colorimetric device

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CN201410635755.9A CN104353509B (en) 2014-11-12 2014-11-12 Many reagent color comparison tube for portable colorimetric device

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CN108792258A (en) * 2018-05-21 2018-11-13 南京申友生物技术有限公司 A kind of genetic test kit

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