CN114132750B - Fluorescence detection system conveying system - Google Patents
Fluorescence detection system conveying system Download PDFInfo
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- CN114132750B CN114132750B CN202210105563.1A CN202210105563A CN114132750B CN 114132750 B CN114132750 B CN 114132750B CN 202210105563 A CN202210105563 A CN 202210105563A CN 114132750 B CN114132750 B CN 114132750B
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- lens
- detection
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/74—Feeding, transfer, or discharging devices of particular kinds or types
- B65G47/90—Devices for picking-up and depositing articles or materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G43/00—Control devices, e.g. for safety, warning or fault-correcting
- B65G43/08—Control devices operated by article or material being fed, conveyed or discharged
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
Abstract
The invention relates to the field related to fluorescence detection, in particular to a fluorescence detection system conveying system; the fluorescence detection system conveying system comprises a sample conveying belt, a detection device arranged at the tail end of the sample conveying belt, a plurality of lenses arranged in the detection device, mounting columns arranged at the bottoms of the lenses, a plurality of bearing columns arranged in the detection device and cleaning columns arranged at two sides of the mounting columns; when the sample conveyer belt conveys a sample to be detected into the detection device for detection, and the detection device is heated up, the compound at the bottom of the sliding groove is heated and expanded, the sliding cylinder is pushed to rotate, and the detection is stopped; the detection failure caused by high temperature during detection can be effectively prevented.
Description
Technical Field
The invention relates to the field related to fluorescence detection, in particular to a fluorescence detection system conveying system.
Background
In the document of chinese patent CN103735249B, a fluorescence detector is disclosed, which comprises a laser beam combining module, a confocal scanning module, a spectral imaging module, a fluorescence microscope and a control display module, wherein the laser beam combining module comprises two near infrared continuous lasers, a laser collimating mirror, a reflecting mirror and a beam combining mirror, the confocal scanning module comprises three dichroic mirrors, three optical filters, a scanning galvanometer, a scanning lens, a pinhole lens and a pinhole which can be switched with each other, the spectral imaging module comprises a beam splitting prism, a focusing mirror, three slits and three photomultiplier tubes, the fluorescence microscope comprises a total reflection prism, a barrel mirror, a microscope objective, a nano displacement table and a sample table, the control display module is used for controlling the laser, the scanning galvanometer, the photomultiplier tubes and the nano displacement table and displaying images, and the fluorescence detector can break through the defect of low detection depth in the prior art, the method realizes centimeter-level fluorescence detection, can simultaneously realize two functions of fluorescence positioning and component analysis, and has wide application prospect in the field of in-vivo fluorescence detection.
The wafers in this patent, however, require conditioning during routine use and cleaning to ensure accuracy of the test.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the problem of lens dispensing in the prior art is overcome, and a fluorescence detection system conveying system is provided.
The invention provides a fluorescence detection system conveying system which comprises a sample conveying belt, a detection device arranged at the tail end of the sample conveying belt, a plurality of lenses arranged in the detection device, mounting columns arranged at the bottoms of the lenses, a plurality of bearing columns arranged in the detection device and cleaning columns arranged at two sides of the mounting columns, wherein the mounting columns are arranged at the bottom of the lens;
the bearing columns correspond to the lenses one by one;
the side surface of the mounting column is a plane;
the interface of the cleaning column is arc-shaped;
the mounting column and the cleaning columns on the two sides of the mounting column form a sliding cylinder;
the upper surface of the bearing column is provided with a sliding groove, the sliding cylinder is slidably arranged in the sliding groove, and the bottom of the sliding groove is provided with a lifting thread groove;
a first cambered surface edge is arranged on the outer side surface of the cambered surface of the mounting column, and a second cambered surface edge is arranged on the cambered surfaces of the two cleaning columns;
the first cambered surface ridge and the two second cambered surface ridges are connected to form a rotating thread, and the rotating thread can be in threaded connection with the lifting thread groove;
when the detection device is ready for detection, a red thermal radiation light source is guided into the detection device, the compound at the bottom of the sliding groove is heated and expands, the sliding cylinder is pushed to pass through the lifting threaded groove and the sliding groove, and at the moment, the red thermal radiation light source can irradiate from each outlet of the detection device;
when the sample conveyer belt conveys a sample to be detected into the detection device for detection, and the detection device is heated up, the compound at the bottom of the sliding groove is heated and expanded, the sliding cylinder is pushed to rotate, and the detection is stopped;
when the detected sample is conveyed away by the sample conveying belt, cleaning steam with a certain temperature is introduced into the detection device, the compound at the bottom of the sliding groove is heated to expand, the sliding cylinder is pushed to rotate until the lens on the mounting column is abutted against the inner wall of the detection device, and at the moment, the two cleaning columns rise along the side faces of the bearing columns to clean the lens; when the cleaning is finished, the two cleaning columns can slide downwards along the side surfaces of the bearing columns to clean the lenses for the second time.
Further, the fluorescence detection system conveying system also comprises an installation plate arranged on the installation column;
the end parts of the two side edges of the mounting plate are bent into a wind sweeping surface;
when the sliding cylinder passes through during the lifting thread groove, the windsweeper of mounting panel both sides can extrude the air, lets the air blow to the lens.
Further, the mounting plate comprises a mounting main plate and wind sweeping side plates arranged on two sides of the mounting main plate;
one side surface of the mounting main board is fixedly connected with the bottom surface of the lens, and the other end of the mounting main board is fixedly connected with the upper surface of the mounting column;
furthermore, a plurality of limiting grooves are formed in the end face of the bearing column at equal intervals;
the end part of the wind sweeping side plate of the mounting plate is clamped in the limiting groove.
Further, the detection device comprises a test base, a transmitting and receiving assembly arranged on one side of the test base and a detection assembly arranged on the other side of the test base;
the test base is opposite to the outlet of the sample conveyer belt;
the bearing columns are respectively arranged in the transmitting and receiving assembly and the detecting assembly.
Further, the transmitting and receiving assembly comprises a transmitting and receiving frame and a fixing plate arranged on the side surface of the transmitting and receiving frame;
a plurality of bearing columns are arranged in the transmitting and receiving frame;
the lens can be abutted against the inner side surface of the fixing plate.
Furthermore, the transmitting and receiving frame is internally provided with a transmitting installation groove, a first LED, a first lens, a first optical filter, a first lens, a second LED, a second lens, a second optical filter, a third LED, a third lens, a third optical filter, a second lens, a third lens, a fourth optical filter, a fourth lens, a fourth LED and a first cylindrical mirror, wherein the first LED, the first lens, the first optical filter, the third lens, the second lens, the fourth optical filter, the fourth lens and the first cylindrical mirror are arranged in the transmitting installation groove;
when the LED I is bright, light passes through the first lens, the first optical filter, the first lens and the third lens for reflection, and finally reaches a sample through the first cylindrical mirror;
when the LED II is bright, light passes through the lens II and the optical filter II, is reflected by the lens III, is reflected by the lens II, passes through the cylindrical mirror I and finally reaches a sample;
when the LED III is bright, light passes through the lens III, the optical filter III, the lens II, the lens III and the cylindrical lens I to reach a sample;
when the LED is bright, light passes through the lens IV and the optical filter IV, is reflected by the lens II, then passes through the lens III, and finally reaches a sample through the cylindrical lens I.
Further, the detection assembly comprises a detection frame, a receiving groove arranged in the detection frame, a first PD, a second PD, a third PD, a fourth PD, a fifth lens, a fifth optical filter, a fourth optical filter, a sixth lens, a seventh optical filter, a fifth optical filter, a sixth optical filter, an eighth lens and a second cylindrical mirror, wherein the first PD, the second PD, the third PD, the fourth PD, the fifth lens, the fifth optical filter, the fourth optical filter, the sixth lens, the seventh lens, the fifth lens, the sixth lens, the eighth lens and the second cylindrical mirror are arranged in the receiving groove;
after the LED I is lighted up, light enters from the cylindrical mirror II, is reflected by the lens IV, passes through the lens IV, then passes through the optical filter VI and the lens VI, and finally reaches the PD I;
after the light of the LED II is lightened, the light enters from the cylindrical mirror II, is reflected by the lens IV, passes through the optical filter seventh and the lens seventh and finally reaches the PD II;
after the LED III light is lightened, the light enters from the cylindrical lens II, passes through the lens VI, then passes through the lens V, passes through the optical filter II and the lens VIII, and finally reaches the PD III;
after the light of the LED four is lightened, the light enters from the cylindrical mirror two, passes through the lens six, is reflected by the lens five, passes through the optical filter eight and the lens eight, and finally reaches the PD four.
Further, the bearing columns are respectively arranged on the bottom surfaces of the receiving groove and the launching mounting groove.
Further, the fluorescence detection system conveying system also comprises heat-conducting silica gel;
one end of the heat-conducting silica gel is fixedly connected with the bottom surface of the receiving groove or the bottom surface of the transmitting mounting groove, and the other end of the heat-conducting silica gel is fixedly connected with the bottom surface of the bearing column.
The invention has the advantages that the quality of the detection device can be intuitively known through the lifting of the mounting column in the detection device in the bearing column, and the detection result is prevented from being wrong under the condition that the sensor is damaged.
Drawings
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of the structure at the detection assembly of the present invention;
FIG. 3 is a schematic diagram of the structure at the transceiver module of the present invention;
FIG. 4 is a schematic view of the structure at the mounting post of the present invention;
fig. 5 is a schematic view of the structure at the mounting plate of the present invention.
In the figure: 100. a sample conveyor belt; 101. a detection device; 1011. testing the base; 1012. a transmit receive assembly; 1012a, a transmitting and receiving frame; 1012b, fixing plates; 1012c, LED one; 1012d, lens one; 1012e, a first filter; 1012g and a second LED; 1012h, lens two; 1012i and a second filter; 1012j, LED three; 1012k, lens three; 1012l and a third optical filter; 1012o and a fourth filter; 1012p, lens four; 1012q and four LEDs; 1013. a detection component; 1013a, PD I; 1013b, PD II; 1013c and PD III; 1013d and PD IV; 1013e, lens five; 1013f and a fifth optical filter; 1013g, a detection frame; 1013h and a sixth optical filter; 1013i and a sixth lens; 1013j, lens seven; 1013k and a seventh optical filter; 1013n and an eight optical filter; 1013o, lens eight; 1014. a first cylindrical mirror; 1015. a second cylindrical mirror; 102. a lens; 102a, a first lens; 102b and a second lens; 102c, a third lens; 102d and a lens four; 102e, lens five; 102f, lens six; 103. mounting a column; 104. a load bearing column; 105. cleaning the column; 106. a first spring; 107. mounting a plate; 108. positioning a plate; 1081. positioning the circular ring; 1082. positioning the bump; 109. installing a circular ring; 110. mounting a bump; 111. a robot arm.
Detailed Description
The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic views illustrating only the basic structure of the present invention in a schematic manner, and thus show only the constitution related to the present invention.
In order to facilitate understanding of the related technical features of the present invention, the related technical features of the present invention will now be described, and the fluorescence detection is a spontaneous luminescence reaction, which can detect human cells, bacteria, mold, and food debris by reacting luciferase with ATP, and obtain a reaction result within 15 seconds. Illuminance, measured by a dedicated device and represented in digital form, was first applied to the food industry in 1975 and was applied to the cosmetic manufacturing industry in 1985. The main drawback of fluorescence detection is that only a few compounds produce fluorescence. Most molecules do not fluoresce but contain derivatizable functional groups for synthesis of derivatives that fluoresce, for example, ortho-phthalaldehyde is a commonly used fluorophore for post-column derivatization of amino acids. Although fluorescence detection is very sensitive, such high sensitivity is not required for common sample analysis. Because the response of ELSDs is independent of fluorophores, no derivatization is required, thus greatly reducing sample pretreatment and analysis time.
In addition, due to the above-described fluorescence detection property, the temperature and humidity of the detection environment should be kept within the error range of the standard value at the time of detection of the sample.
As shown in fig. 1 to 5, the fluorescence detection system conveying system of the present invention includes a sample conveying belt 100, a detection device 101 disposed at the end of the sample conveying belt 100, a plurality of lenses 102 disposed in the detection device 101, a mounting column 103 disposed at the bottom of the lenses 102, a plurality of bearing columns 104 disposed in the detection device 101, and cleaning columns 105 disposed at two sides of the mounting column 103; wherein the bearing post 104 is located below each of the lenses 102. The conveyor belt is adapted to convey the vessels filled with the samples. The detection device 101 is adapted to emit light of different wavelengths and direct the light to impinge on the sample and reflect from the sample back into the detection device 101, the detection device 101 being capable of detecting the analyzed sample according to the different light. The mounting post 103 can be internally provided with the bearing post 104 and the cleaning post, and the mounting post 103 can be matched with the bearing post 104 and the cleaning post to adjust the position of the lens 102, so that whether each branch in the detection device 101 is unobstructed and whether each sensor is intact can be judged. The upper component is described in detail below.
Conveying section
The conveyor belt can cyclically slide in a single direction, so that the sample vessels placed on the conveyor belt are sequentially conveyed to the detection device 101. One side of the detection device 101 is provided with a manipulator 111, and after the sample on the detection device 101 is detected, the manipulator 111 can take the sample vessel away. And because the result can be obtained within 15 seconds of the fluorescence detection, the sample vessel on the conveying belt and the grabbing interval of the manipulator 111 are correspondingly set to be about 15 seconds, so that the best detection efficiency can be achieved.
Detecting part
The detection device 101 can respectively irradiate different wavelengths onto the sample, and refract the sample into the detection device 101 for detection after passing through the sample, so that the sample can be effectively detected. The detection device 101 comprises a test base 1011, a transmitting and receiving component 1012 arranged on one side of the test base 1011, and a detection component 1013 arranged on the other side of the test base 1011. In this embodiment, the emitting device can emit four kinds of fluorescence with different wavelengths, the detecting device 101 can receive the four kinds of fluorescence with different wavelengths, and the parts of the fluorescence emitted by the emitting device correspond to the parts of the detecting device 101 that receive the fluorescence one by one. The test base 1011 faces the outlet of the sample conveyor belt 100, when a sample vessel moves to the tail end along with the conveyor belt, a sample slides to the central position of the test base 1011 under the action of inertia, and at the moment, fluorescence emitted from the emitting device can irradiate on the sample and returns to the detection device 101 through sample refraction. The lens 102 in the present invention is a dichroic mirror;
the invention is provided with a plurality of dichroic mirrors, the structures of which are the same and are positioned in the relevant light paths convenient for understanding in the application, and the dichroic mirrors in the year of the application are respectively endowed with corresponding marks; that is, the structure of the lens 102 is the same as that of the first lens 102a, the second lens 102b, the third lens 102c, the fourth lens 102d, the fifth lens 102e and the sixth lens 102 f;
the structure of the transceiver module 1012 will be described in detail below, and the transceiver module 1012 includes a transceiver frame 1012a and a fixing plate 1012b disposed on a side of the transceiver frame 1012 a. A number of the support columns 104 are disposed within the transceiver frame 1012 a; the fixing plate 1012b can cooperate with the transceiver frame 1012a so that various fluorescent lights emitted within the transceiver assembly 1012 do not diverge outwardly. The transmitting and receiving frame 1012a is internally provided with a transmitting and receiving groove, a first LED 1012c, a first lens 1012d, a first filter 1012e, a first lens 102a, a second LED 1012g, a second lens 1012h, a second filter 1012i, a third LED 1012j, a third lens 1012k, a third filter 1012l, a second lens 102b, a third lens 102c, a fourth filter 1012o, a fourth lens 1012p, a fourth LED 1012q and a first cylindrical mirror 1014. The specific fluorescence emission path is as follows:
when the first LED 1012c is bright, light passes through the first lens 1012d and the first filter 1012e, passes through the first lens 102a and is reflected by the third lens 102c, and finally reaches a sample through the first cylindrical mirror 1014;
when the second LED 1012g is bright, light passes through the second lens 1012h and the second optical filter 1012i, is reflected by the third lens 102c, is reflected by the second lens 102b, passes through the first cylindrical mirror 1014 and finally reaches a sample;
when the LED III 1012j is bright, light passes through the lens III 1012k, the filter III 1012l, the lens II 102b, the lens III 102c and the cylindrical lens I1014 to reach a sample;
when the LED four 1012q is bright, light passes through the lens four 1012p and the filter four 1012o, is reflected by the lens two 102b, passes through the lens three 102c, and finally reaches the sample through the cylindrical mirror one 1014.
The structure of the detecting assembly 1013 is described in detail below, where the detecting assembly 1013 includes a detecting frame 1013g, a receiving groove provided inside the detecting frame 1013g, PD one 1013a, PD two 1013b, PD three 1013c, PD four 1013d arranged inside the receiving groove, a lens five 1013e, a filter five 1013f, a lens four 102d, a filter six 1013h, a lens six 1013i, a lens seven 1013j, a filter seven 1013k, a lens five 102e, a lens six 102f, a filter eight 1013n, a lens eight 1013o, and a cylindrical mirror 1015; the specific fluorescence receiving path is as follows:
after the first LED 1012c is lighted up, light enters from the second cylindrical mirror 1015, is reflected by the fourth lens 102d, passes through the third lens 102c, passes through the sixth filter 1013h and the sixth lens 1013i, and finally reaches the first PD 1013 a;
after the light of the second LED 1012g is turned on, the light enters from the second cylindrical mirror 1015, is reflected by the fourth lens 102d, is reflected by the third lens 102c, passes through the seventh optical filter 1013k and the seventh lens 1013j, and finally reaches the second PD 1013 b;
after the light of the LED three 1012j is turned on, the light enters from the second cylindrical mirror 1015, passes through the sixth lens 102f, passes through the fifth lens 102e, passes through the eight optical filter 1013n and the eight lens 1013o, and finally reaches the PD three 1013 c;
after the LED four 1012q is lit, light enters from the second cylindrical mirror 1015, passes through the sixth mirror 102f, is reflected by the fifth mirror 102e, passes through the filter eight 1013n, the lens eight 1013o, and finally reaches the PD four 1013 d.
In the above emission and structure process, it should be noted that the first lens 102a can transmit the light of the first LED 1012c and reflect the light of the second LED 1012 g; the second lens 102b can transmit the light of the LED three 1012j and the LED four 1012q and reflect the light of the LED one 1012c and the LED one 1012 c; the third lens 102c can transmit the light of the third LED 1012j and reflect the light of the fourth LED 1012 q; the lens four 102d five-way mirror can transmit the light of the LED one 1012c and reflect the light of the LED two 1012 g; lens five 102e can transmit light of LED three 1012j and LED four 1012q and reflect light of LED one 1012c and LED two 1012 g; lens four 102d six is capable of transmitting LED three 1012j light and reflecting LED four 1012q light. In this way, the light emitted by the LED one 1012c can be received by the PD one 1013a, and similarly, the light emitted by the LED two 1012g can be received by the PD two 1013 b; light emitted by LED three 1012j can be received by PD three 1013 c; the light emitted by LED four 1012q can be received by PD four 1013 d. Thereby realizing the layered detection of the sample.
The plurality of bearing columns 104 are respectively disposed in the emission receiving component 1012 and the detection component 1013, the plurality of bearing columns 104 are respectively disposed on the receiving groove and the bottom surface of the emission mounting groove, one bearing column 104 corresponds to one lens 102, the lens 102 is slidably disposed on one side of the bearing column 104, and the lens 102 can slide to abut against the fixing plate 1012 b. In order to ensure that the bearing column 104 reliably transfers heat with the receiving groove and the bottom surface of the emission mounting groove, the fluorescence detection system conveying system further comprises heat-conducting silica gel; one end of the heat-conducting silica gel is fixedly connected with the bottom surface of the receiving groove or the bottom surface of the transmitting mounting groove, and the other end of the heat-conducting silica gel is fixedly connected with the bottom surface of the bearing column 104. The upper surface of the bearing column 104 is provided with a sliding groove.
Two relative sides of erection column 103 are the plane, and two other sides that back on the back are second cambered surface arris, and two clearance post 105 one side is the plane, and another side is arc, and the plane of clearance post 105 and the plane looks adaptation of erection column 103, erection column 103 with the clearance post 105 of erection column 103 both sides constitutes a slip cylinder. The outer diameter of the sliding cylinder is matched with the outer diameter of the sliding groove, so that the sliding cylinder can be arranged in the sliding groove in a sliding mode. In addition, a lifting thread groove is formed in the bottom of the sliding groove, the arc surfaces at the two ends of the mounting column 103 are first arc surface edges, and the first arc surface and the second arc surface can form a cylinder. The height of the thread groove is one fourth of the length of the load post 104, and the first cambered surface ridge and the second cambered surface ridge thread groove are matched. The first cambered surface arris and the two second cambered surface arris are connected to form a rotating thread, and the rotating thread can be in threaded connection with the lifting thread groove. The lens 102 is rotatably connected with one end of the mounting column 103 far away from the first cambered surface edge. In this way, during the process that the mounting post 103 links the two cleaning posts 105 to spirally slide in the bearing post 104, the mounting post 103 can drive the corresponding lens 102 to synchronously and spirally slide along the axial direction of the bearing post 104 so as to move against the fixing plate 1012b or move away from the fixing plate 1012 b. Specifically, when the lens 102 moves to abut against the fixing plate 1012b, the first and second curved surfaces move to disengage from the threaded groove.
In order to facilitate the control of the lifting of the mounting post 103 and the cleaning post 105, the bottom of the bearing post 104 is provided with a compound which can expand by heating to exert an axial force on the mounting post 103 and the cleaning post 105 so that the cleaning post 105 and the mounting post 103 slide axially through the first cambered surface edge and the second cambered surface edge. In this way, before the sample is formally tested, it can be tested whether the light paths of the emission-receiving component 1012 and the detection component 1013 are smooth or not. Specifically, the outer wall of the support column 104 is supported by a black heat absorbing material, a heat radiation light source in red color is introduced into the sensing member 1013 through a receiving groove and the emission mounting groove or sensing groove, so that when the red heat radiation light source irradiates the outer side of the corresponding support column 104, the support column 104 transfers heat to the compound, so that the compound expands when heated, pushes the corresponding cleaning column 105 and the mounting column 103 to spirally slide outwards, when red heat radiation light irradiates the corresponding lens 102, the lens 102 is spirally rotated with the mounting post 103, so that the red heat radiation light is radiated to the periphery, and then the red heat radiation light irradiates on other bearing columns 104 in the emission mounting groove or the detection groove, each bearing column 104 transfers heat to the corresponding compound, so that the compound is heated and expanded, the corresponding cleaning column 105 and the mounting column 103 are pushed to slide outwards in a spiral manner, and the red heat radiation light is continuously diffused to the periphery. Finally, the red heat radiation light passes through each transmitting installation groove or detecting groove corresponding to the transmitting and receiving component 1012 or the detecting component 1013, if the transmitting and receiving component 1012 or the detecting component 1013 generates flickering red light, the light path of the transmitting installation groove or the detecting groove corresponding to the flickering red light is unblocked, and if the transmitting and receiving component 1012 or the detecting component 1013 does not generate flickering red light, the light path of the transmitting installation groove or the detecting groove corresponding to the flickering red light is blocked, so that the maintenance is required. Meanwhile, when the detection groove is detected, whether red light flickers or not can be observed, whether each PD end is read or not can be observed, whether the PD end is intact or not can be judged, and if the PD end is not intact, the PD end needs to be replaced or repaired. In addition, since the fluorescent light is invisible light, a heat radiation light source of red color is used in operation.
The two cleaning columns 105 are also correspondingly provided with cleaning brushes on one side close to the lens 102, when the mounting column 103 slides to be separated from the thread groove, the lens 102 is abutted against the fixing plate 1012b, at the moment, the cleaning columns 105 on the two sides are also separated from the thread operation, the cleaning columns 105 can axially slide along the bearing column 104, and the corresponding brushes can clean the two side faces of the mounting column 103. One end of each cleaning column 105 far away from the lens 102 is fixed with a spring one 106, and the other end of the spring one 106 is fixed with the bottom of the bearing column 104. After the compound cools, the first spring 106 can pull the corresponding cleaning post 105 to retract to the lower end flush with the mounting post 103, and the two cleaning posts 105 slide down along the side surface of the bearing post 104 to clean the lens 102 for the second time. After the mounting post 103 is flush with the cleaning post 105, the compound continues to cool, creating a negative pressure at the bottom of the load-bearing post 104, and the atmospheric pressure pushes the mounting post 103 and the cleaning post 105 to slide back to the bottom of the load-bearing post 104.
The fluorescence detection system conveying system further comprises a mounting plate 107 arranged on the mounting column 103; the end parts of the two side edges of the mounting plate 107 are bent into a wind sweeping surface; when the sliding cylinder passes through the lifting screw groove, the air is squeezed by the windward surfaces on both sides of the mounting plate 107, and the air is blown toward the lens 102. The mounting plate 107 comprises a mounting main plate and wind sweeping side plates arranged on two sides of the mounting main plate; one side surface of the mounting main board is fixedly connected with the bottom surface of the lens 102, and the other end of the mounting main board is fixedly connected with the upper surface of the mounting column 103. The erection column 103 drives the gliding in-process of lens 102 spiral, and mounting panel 107 can the blast air, clears up the impurity on the lens 102 in advance, cooperates clearance brush clearance lens 102 surface again and glues glutinous impurity to reduce the influence of impurity to the testing result.
In order to facilitate the adjustment of the angle of the lens 102, a plurality of limiting grooves are equidistantly formed in the end surface of the bearing column 104; the end part of the wind sweeping side plate of the mounting plate 107 is clamped in the limiting groove. A positioning plate 108 is correspondingly arranged on one side of the mounting column 103, the positioning plate 108 is matched with a mounting main board, a positioning ring 1081 and a positioning bump 1082 arranged in the positioning ring 1081 are arranged on the positioning plate 108, and a mounting ring 109 and a mounting bump 110 arranged at the center of the mounting ring 109 are arranged at the bottom of the mounting main board; the positioning ring 1081 is rotatably disposed within the mounting ring 109;
and the angle between the positioning projection 1082 and the mounting projection 110 is 180 ° in the initial state. When the lens 102 and the mounting plate 107 rotate relatively, the corresponding wind sweeping side plate is separated from the corresponding limiting groove and clamped into the adjacent limiting groove, so that the 'click' sound is generated. Because the limiting grooves are uniformly arranged along the circumferential direction of the bearing column 104, the angle of the lens 102 can be judged through the 'click' sound energy. When the mounting protrusion 110 rotates to abut against the mounting protrusion 110, the lens 102 can rotate synchronously with the mounting post 103.
In the detection process, if the signal received by the PD terminal becomes disordered, it indicates that the temperature of the detection environment rises, and when the detection device 101 is heated, the compound (here, living water may be used, and different solvents may be added to the water to change the boiling point of the water) at the bottom of the sliding groove is heated and expands, so as to push the sliding cylinder to rotate, and the fluorescence emitted by the LED is scattered to the periphery. The operator can be alerted to terminate the test at this point.
The working principle of the invention is as follows:
when the detection device 101 is ready for detection, a red thermal radiation light source is introduced into the detection device 101, the compound at the bottom of the sliding groove expands due to heating, and the sliding cylinder is pushed to pass through the lifting screw groove and the sliding groove, and at this time, the red thermal radiation light source irradiates from each outlet of the detection assembly 1013 or the emission receiving assembly 1012;
when the sample conveyer belt 100 sends a sample to be detected to the test base 1011 for detection, and the detection component 1013 or the transmitting and receiving component 1012 heats up, the compound at the bottom of the sliding groove is heated and expands, the sliding cylinder is pushed to rotate, and the detection is stopped;
when the tested sample is conveyed by the sample conveyor belt 100, cleaning steam with a certain temperature is introduced into the testing device 101, the compound at the bottom of the sliding groove expands due to heat, and pushes the sliding cylinder to rotate until the lens 102 on the mounting column 103 abuts against the inner wall of the testing component 1013 or the transmitting and receiving component 1012, and at this time, the two cleaning columns 105 will rise along the side surfaces of the bearing column 104 to clean the lens 102; when the cleaning process is finished, the two cleaning posts 105 slide down the side of the carrying post 104 to clean the lens 102 for the second time.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. A fluorescence detection system conveying system is characterized by comprising a sample conveying belt, a detection device arranged at the tail end of the sample conveying belt, a plurality of lenses arranged in the detection device, mounting columns arranged at the bottoms of the lenses, a plurality of bearing columns arranged in the detection device and cleaning columns arranged at two sides of the mounting columns;
the bearing columns correspond to the lenses one by one;
the side surface of the mounting column is a plane;
the interface of the cleaning column is arc-shaped;
the mounting column and the cleaning columns on the two sides of the mounting column form a sliding cylinder;
the upper surface of the bearing column is provided with a sliding groove, the sliding cylinder is slidably arranged in the sliding groove, and the bottom of the sliding groove is provided with a lifting thread groove;
a first cambered surface edge is arranged on the outer side surface of the cambered surface of the mounting column, and a second cambered surface edge is arranged on the cambered surfaces of the two cleaning columns;
the first cambered surface ridge and the two second cambered surface ridges are connected to form a rotating thread, and the rotating thread can be in threaded connection with the lifting thread groove;
when the detection device is ready for detection, a red thermal radiation light source is guided into the detection device, the compound at the bottom of the sliding groove is heated and expands, the sliding cylinder is pushed to pass through the lifting threaded groove and the sliding groove, and at the moment, the red thermal radiation light source can irradiate from each outlet of the detection device;
when the sample conveyer belt conveys a sample to be detected into the detection device for detection, and the detection device is heated up, the compound at the bottom of the sliding groove is heated and expanded, the sliding cylinder is pushed to rotate, and the detection is stopped;
when the detected sample is conveyed away by the sample conveying belt, cleaning steam with a certain temperature is introduced into the detection device, the compound at the bottom of the sliding groove is heated to expand, the sliding cylinder is pushed to rotate until the lens on the mounting column is abutted against the inner wall of the detection device, and at the moment, the two cleaning columns rise along the side faces of the bearing columns to clean the lens; when the cleaning is finished, the two cleaning columns can slide downwards along the side surfaces of the bearing columns to clean the lenses for the second time.
2. The fluorescence detection-system delivery system according to claim 1,
the fluorescence detection system conveying system also comprises an installation plate arranged on the installation column;
the end parts of the two side edges of the mounting plate are bent into a wind sweeping surface;
when the sliding cylinder passes through during the lifting thread groove, the windsweeper of mounting panel both sides can extrude the air, lets the air blow to the lens.
3. The fluorescence detection-system delivery system according to claim 2,
the mounting plate comprises a mounting main plate and wind sweeping side plates arranged on two sides of the mounting main plate;
one side surface of the mounting main board is fixedly connected with the bottom surface of the lens, and the other end of the mounting main board is fixedly connected with the upper surface of the mounting column.
4. The fluorescence detection-system delivery system according to claim 3,
a plurality of limiting grooves are formed in the end face of the bearing column at equal intervals;
the end part of the wind sweeping side plate of the mounting plate is clamped in the limiting groove.
5. The fluorescence detection-system delivery system according to claim 4,
the detection device comprises a test base, a transmitting and receiving assembly arranged on one side of the test base and a detection assembly arranged on the other side of the test base;
the test base is opposite to the outlet of the sample conveyer belt;
the bearing columns are respectively arranged in the transmitting and receiving assembly and the detecting assembly.
6. The fluorescence detection-system delivery system according to claim 5,
the transmitting and receiving assembly comprises a transmitting and receiving frame and a fixing plate arranged on the side surface of the transmitting and receiving frame;
a plurality of bearing columns are arranged in the transmitting and receiving frame;
the lens can be abutted against the inner side surface of the fixing plate.
7. The fluorescence detection-system delivery system according to claim 6,
the transmitting and receiving frame is internally provided with a transmitting and receiving groove, a first LED, a first lens, a first optical filter, a first lens, a second LED, a second lens, a second optical filter, a third LED, a third lens, a third optical filter, a second lens, a third lens, a fourth optical filter, a fourth lens, a fourth LED and a first cylindrical mirror, wherein the first LED, the first lens, the first optical filter, the first lens, the third optical filter, the fourth lens and the first cylindrical mirror are arranged in the transmitting and receiving groove;
when the LED I is bright, light passes through the first lens, the first optical filter, the first lens and the third lens for reflection, and finally reaches a sample through the first cylindrical mirror;
when the LED II is bright, light passes through the lens II and the optical filter II, is reflected by the lens III, is reflected by the lens II, passes through the cylindrical mirror I and finally reaches a sample;
when the LED III is bright, light passes through the lens III, the optical filter III, the lens II, the lens III and the cylindrical lens I to reach a sample;
when the LED is bright, light passes through the lens IV and the optical filter IV, is reflected by the lens II, then passes through the lens III, and finally reaches a sample through the cylindrical lens I.
8. The fluorescence detection-system delivery system according to claim 7,
the detection assembly comprises a detection frame, a receiving groove arranged in the detection frame, a first PD, a second PD, a third PD, a fourth PD, a fifth lens, a fifth optical filter, a fourth optical lens, a sixth optical filter, a sixth lens, a seventh optical filter, a fifth optical lens, a sixth optical lens, an eighth optical filter, an eighth lens and a second cylindrical mirror, wherein the first PD, the second PD, the third PD, the fourth PD, the fifth lens, the fourth lens, the sixth optical filter, the sixth lens, the seventh optical filter, the fifth lens, the sixth lens, the eighth lens and the second cylindrical mirror are arranged in the receiving groove;
after the LED I is lighted up, light enters from the cylindrical mirror II, is reflected by the lens IV, passes through the lens IV, then passes through the optical filter VI and the lens VI, and finally reaches the PD I;
after the light of the LED II is lightened, the light enters from the cylindrical mirror II, is reflected by the lens IV, passes through the optical filter seventh and the lens seventh and finally reaches the PD II;
after the LED III light is lightened, the light enters from the cylindrical lens II, passes through the lens VI, then passes through the lens V, passes through the optical filter II and the lens VIII, and finally reaches the PD III;
after the light of the LED four is lightened, the light enters from the cylindrical mirror two, passes through the lens six, is reflected by the lens five, passes through the optical filter eight and the lens eight, and finally reaches the PD four.
9. The fluorescence detection-system delivery system according to claim 8,
the bearing columns are respectively arranged on the receiving groove and the bottom surface of the launching mounting groove.
10. The fluorescence detection-system delivery system according to claim 9,
the fluorescence detection system conveying system also comprises heat-conducting silica gel;
one end of the heat-conducting silica gel is fixedly connected with the bottom surface of the receiving groove or the bottom surface of the transmitting mounting groove, and the other end of the heat-conducting silica gel is fixedly connected with the bottom surface of the bearing column.
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