CN1141017C - Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China - Google Patents
Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China Download PDFInfo
- Publication number
- CN1141017C CN1141017C CNB001054880A CN00105488A CN1141017C CN 1141017 C CN1141017 C CN 1141017C CN B001054880 A CNB001054880 A CN B001054880A CN 00105488 A CN00105488 A CN 00105488A CN 1141017 C CN1141017 C CN 1141017C
- Authority
- CN
- China
- Prior art keywords
- type
- wheat
- sowing
- sterile
- sterile line
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a method for selectively breeding temperature-sensitive sterile line of wheat suitable for the Yellow River wheat region and the Huai River wheat region in China. A wheat not 1B/1R type K type sterile line is selectively bred by another patent of invention (ZL94116537. X) of He Peiru, wherein the short arms of 1B chromosomes of some individuals have fertile regulation and control genes which are abnormally sensitive to the temperature of the Yellow River wheat region and the Huai River wheat region. A base temperature sensitive sterility material ETS which is suitable for the ecological conditions of Yellow River wheat region and the Huai River wheat region is selectively bred according to the selection standard of complete sterility of autumn sowing and more than 40% of selfing fertility of spring sowing in the Yellow River wheat region and the Huai River wheat region. The ETS serves as a female parent which is hybridized and backcrossed with any wheat breed; the fertile strains which have the characteristics of similarity to the traits of recurrent parents, complete sterility in autumn sowing, more than 40% of selfing fertility in spring sowing and recovery of the T-shaped wheat sterile line are selectively reserved, and are the wheat temperature-sensitive sterile line which is suitable for the Yellow River wheat region and the Huai River wheat region. The seed breeding programs of the hybrid wheat is simplified by the method of the present invention, and the problems of high cost of sterile line seed breeding in triple crossing wheat seed production and difficult preservation of pure seed are solved.
Description
Technical field
The present invention relates to wheat heterosis and utilize the field, specifically, relate to a kind of method of seed selection wheat responsive to temperature male sterile line.
Background technology
Japanese scholar in 1978 often coerces this inferior that of a permanent Lang Yucheng wheat wild mutation and takes off the K type male sterile wheat line that (T.spelta) keeps, but the male sterile line that wild variety keeps is difficult to be used for hybrid wheat production.He Beiru etc. utilized the good wheat breed of 1R/1R type of China to breed 1B/1R type K type male sterile line in 1987, and had been used for Wheat Production.But the forfeiture of 1B/1R type disease-resistant wheat, bad, Yi Sui germinate, thereby the maintenance line resource is fewer and feweri, has limited the utilization of K type male sterile line.
He Bei a kind ofly relates to spelt 1B the short arm of a chromosome in the T type and recovers gene Rf as inventing in 1994
3With K type sterile gene rfk
1Fragment transfer to good wheat cdna group, the method (Chinese invention patent ZL94116537.x) of the non-1B/1R type of seed selection K type male sterile wheat line.Utilize this invention the good wheat that is suitable for arbitrarily as the hybrid wheat female parent can be modified to non-1B/1R type maintenance line, and then breed male sterile line, the male sterile line of this non-1B/1R type does not produce monoploid, and the recovery source is more extensive, easy restorative raising.It is a kind of comparatively practical " three are " breeding system.
Hybrid wheat utilizes three to be that (sterile line, maintainer, restorer) carries out seed production, need to be under strict isolation condition, and the alternate plantation of maintainer and sterile line, the former is to latter's production male-sterile seed of pollinating.Because of maintenance line and male sterile line except that male fertile is different, proterties is very similar, production process is easy to generate and mixes, and the so little strain large group crop impurity elimination poor effect of wheat, thus the guarantor of numerous kind of male sterile line pure be that hybrid wheat moves towards one of technical barrier of producing on a large scale.Moreover, maintenance line and male sterile line flower synchronization when guaranteeing numerous kind of male sterile line, generally with maintenance line late sowing 7～15 days, cause the maintenance line plant height to reduce, the florescence shortens, and gives maternal poor pollination, the male sterile line outcrossing seed-setting rate is low, seed yield is few, and male-sterile seed cost height is totally unfavorable for the hybrid wheat industrialization.
Since the seventies, the agricultural science and technology worker of China has successively found the temperature and the photoperiod-sensitive phenomenon of male fertile in chief crops such as paddy rice, Chinese sorghum, corn, wheat.Warm, light activated crop male sterility ties up to produces upper positive season cultivation performance male sterility, and the Inverse season for culturing then has certain selfing fertility, realizes that numerous kind of sterile line no longer needs the maintainer pollination; In numerous kind of this anti-season, the sterile line of in season seed breeding removes maintainer, and it is pure that numerous kind of sterile line can be protected safely, improves output, reduces cost.
Hunan China agricultural university in 1992, Chongqing City crop find temperature, the photosensitive sterile line of wheat in succession and be used for hybrid wheat seed selection (Hybrid Wheat, China Agricultural University Press, 1998, Beijing, 40-53,316-320).It is sterile that it does not show holandry in China's wheat main producing region the Yellow River and Huai He River winter wheat district autumn sowing test; Its reason is that this two classes male sterile line is in the seed selection of the Chinese Yangtze river basin, and the fertility inversion temperature is respectively 10～11 ℃, 13～14 ℃, can not express male sterile under 14～16 ℃ of conditions of Yellow River-Huai River region mean temperature of air in booting stage.Male fertile and the ecological conditions such as temperature, light warm, photosensitive male sterile wheat line are closely related, and therefore, are referred to as again ecological male sterility.Yellow River-Huai River region wheat area accounts for 60～70% of China, and temperature, photaesthesia wheat male sterile line that seed selection adapts to this area have science and important meaning economically.
Summary of the invention
The purpose of this invention is to provide and a kind of any good wheat breed (being) transformation easily become is adapted to the method for the non-1B/1R type of the temperature sensitive wheat K type male sterile line of Yellow River-Huai River region ecological condition, to simplify the hybrid wheat production routine.
Seed selection adapts to the method for the non-1B/1R type of the responsive to temperature type wheat K type male sterile line in the Yellow River and Huai He River winter wheat district, comprise the non-1B/1R type K type male sterile wheat line that utilizes the seed selection of existing method (ZL94116537.x) institute, screening adapts to the basic thermo-sensitive sterile material ETS of the Yellow River and Huai He River winter wheat district ecological condition, utilize basic thermo-sensitive sterile material ETS and any good wheat breed hybridization, and then backcross transformation becomes the temp-sensing sterile line of this kind.
The inventor studies confirm that, exists T-shaped sterile line to recover gene Rf on the spelt 1B the short arm of a chromosome
3With with its chain K type sterile gene rfk
1, some individualities also exist under K type cytoplasm background, vary with temperature the temperature sensitive gene of regulation and control male fertile.Temperature sensitive gene may be present in the 1B the short arm of a chromosome when genetic recombination, also may lose, the non-1B/1R type K type male sterile line that will have spelt 1B the short arm of a chromosome, adopt the individual and breeding that the different temperatures screening is sterile in the Yellow River and Huai He River winter wheat district's autumn sowing holandry, certain selfing fertility is arranged when sowing in spring, can obtain thermo-sensitive sterile material ETS.The ETS material has K type (sticking fruit goatweed, Ae.kotschyi) cytoplasm, and spelt 1B the short arm of a chromosome is arranged in the nuclear, and this chromosome segment has the T type and recovers gene Rf
3, K type sterile gene rfk
1With the temperature sensitive gene of fertility.Under the varieties in Huang-Huai-Hai Plain ecological condition, hybridize, backcross take the ETS material as maternal and good wheat breed, its offspring's autumn sowing, the florescence is selected complete sterile individuality, cut off whole tassels after spending, the individuality that certain selfing fertility is given birth in the regeneration ear selection is the good temperature sensitive K type of non-1B/1R type sterile line.
Content of the present invention is the method that seed selection adapts to the temperature sensitive non-1B/1R type K type male sterile line of the Yellow River and Huai He River winter wheat district's wheat, it is characterized in that utilizing the non-1B/1R type K type male sterile line that has spelt 1B the short arm of a chromosome, under the Yellow River-Huai River region ecological condition, select sterile, the self-fruitful temperature sensitive sterile basic material of sowing in spring of autumn sowing holandry, and then any improved seeds (F.W.V.) transformation is become temperature sensitive non-1B/1R type K type male sterile line.
The method of seed selection basis thermo-sensitive sterile material ETS comprises the steps:
(1) the non-1B/1R type K type male sterile wheat line that will have spelt 1B the short arm of a chromosome is at the Yellow River and Huai He River winter wheat district planted in fall, and the selfing of 1-2 fringe is overlapped in the every strain in heading back, and all the other tillering tassels cut off from base portion 2～3 internodes, and an amount of fertilising is poured water, and promotes to tiller regeneration;
(2) regeneration is tillered heading back by the selfing of fringe bagging, ripe before by strain results self-fertility seed;
(3) with autumn sowing sterile and regeneration fringe can educate single-strain seed in the Yellow River and Huai He River winter wheat district by strain autumn sowing and spring sowing, the strain system that autumn sowing is sterile fully, the spring sowing selfing can be solid of selecting and remain is basic temperature sensitive non-1B/1R type K type male sterile line (ETS);
Any good wheat breed (F.W.V.) is modified to the method for temperature sensitive non-1B/1R type K type male sterile line, belongs to 1B/1K type or non-1B/1R type and utilize diverse ways according to these improved seeds.
The method that the good wheat breed of non-1B/1R type is modified to temperature sensitive non-1B/1R type K type male sterile line comprises the steps:
(1) under the autumn sowing condition, does maternal and the good wheat breed of non-1B/1R type (NON-1B/1R F.W.V.) hybridization, get the F of fertility restorer with basic thermo-sensitive sterile material (ETS)
1Generation;
(2) F
1Get F for selfing
2Generation;
(3) F
2In generation, pulls out and can educate strain in the varieties in Huang-Huai-Hai Plain autumn sowing florescence, and sterile strain is by 1～2 of strain bagging, and backcross by strain and F.W.V., all the other tillering tassels cut off from base portion 2～3 internodes, suitably apply fertilizer and pour water, promote to tiller regeneration, the seed B that backcrosses on the results regeneration tillering tassel self-fertility individual plant
1F
1Generation;
(4) B
1F
1Get B for selfing
1F
2Generation;
(5) B
1F
2In generation, is in the varieties in Huang-Huai-Hai Plain autumn sowing, pull out florescence and can educate strain, sterile strain is by 1～2 of strain bagging, and backcross by strain and F.W.V., all the other tillering tassels cut off from base portion 2～3 internodes, suitably fertilising is poured water, and promotes to tiller regeneration, and results regeneration tillering tassel self-fertility and proterties are near the seed B that backcrosses on the individual plant of F.W.V
2F
1Generation;
(6) B
2F
1Get B for selfing
2F
2Generation;
(7) B
2F
2In generation, is in the varieties in Huang-Huai-Hai Plain autumn sowing, pull out florescence and can educate strain, sterile strain is by 1～2 of strain bagging, and backcross by strain and F.W.V., all the other tillering tassels cut off from base portion 2～3 internodes, suitably fertilising is poured water, and promotes to tiller regeneration, and results regeneration tillering tassel self-fertility and proterties are near the seed B that backcrosses on the individual plant of F.W.V
3F
1Generation;
(8) B
3F
1Get B for selfing
3F
2Generation;
(9) with B
3F
2, regeneration fringe sterile for autumn sowing fringe performance can educate and proterties and F.W.V quasispecies selfed seed in the strain autumn sowing of varieties in Huang-Huai-Hai Plain branch and spring sowing, selecting and remain, autumn sowing is fully sterile, the strain of spring sowing self-fertility, is the temperature sensitive non-1B/1R type K type sterile line of improved seeds F.W.V.
With the method for the temperature sensitive non-1B/1R type K type male sterile line of the good wheat breed of 1B/1R type improvement becoming, divide two kinds of situations and adopt distinct methods.
(1) transformation does not become the 1B/1R type improved seeds F.W.V of K type male sterile line to improve the method that becomes temperature sensitive non-1B/1R type K type male sterile line:
(1) under the autumn sowing condition, take basic temperature sensing material ETS as maternal, with 1B/1R type improved seeds F.W.V hybridize F
1
(2) F
1In the Yellow River-Huai River region autumn sowing, a fringe selfing is stayed in every strain, backcrosses with F.W.V as maternal take 1～2 fringe, and the seed that backcrosses in the complete sterile strain of results selfing gets B
1F
1
(3) B
1F
1In the Yellow River-Huai River region autumn sowing, the florescence cuts off whole tassels from base portion 2～3 internodes, and suitably irrigation fertilization promotes to tiller regeneration;
(4) can educate the regeneration fringe castrate the F.W.V that sows in spring with Yellow River-Huai River region backcross B
2F
1Generation;
(5) B
2F
1Sow in spring for Yellow River-Huai River region, select proterties the educated strain similar to F.W.V and F.W.V backcross B
3F
1
(6) B
3F
1Individual plant is in the varieties in Huang-Huai-Hai Plain autumn sowing, and the selection traits sterile strain similar to F.W.V overlapped 1 fringe selfing by strain; The autumn sowing of selecting and remain is fully sterile, the self-fruitful individual plant of regeneration fringe;
(7) previous generation's individual plant of selecting and remain, by strain divide autumn sowing, 2 processing of sowing in spring, the proterties of selecting and remain is similar to F.W.V, the autumn sowing holandry is sterile, sow in spring can educate strain strain system and with any T type male sterile line test cross of spring sowing;
(8) divide autumn sowing, two processing of spring sowing with roguing system in above-mentioned, autumn sowing simultaneously these strain systems and T type male sterile line test cross F
1, final election autumn sowing holandry is sterile, spring sowing self-fertility, T type male sterile line test cross F
1It is to be the non-1B/1R type K type male sterile line of this 1B/1R kind that the strain of 40～50% ripening rates is arranged.
(2) K type male sterile line (K) F.W.V.A that 1B/1R type kind is kept is modified to the method for temperature sensitive non-1B/1R type K type male sterile line, comprises the steps:
(1) under Yellow River-Huai River region spring sowing condition, the K type that keeps with this kind is sterile be maternal and temperature sensitive basic material (ETS) hybridize F
1
(2) under the spring sowing condition, with F
1Do male parent and (K) F.W.V.A backcross B
1F
1
(3) under the spring sowing condition, with the proterties B close with F.W.V
1F
1Can educate strain do male parent and (K) F.W.V.A backcross B
2F
1
(4) under the spring sowing condition, select the proterties B similar to F.W.V
2F
1Can educate strain do male parent and (K) F.W.V.A backcross B
3F
1
(5) B
3F
1Sow in spring, the similar fertile plant selfing to F.W.V of the proterties of selecting and remain gets B
3F
2
(6) B
3F
2Middle menu strain divides autumn sowing at varieties in Huang-Huai-Hai Plain, two processing of sowing in spring, and the autumn sowing holandry of selecting and remain is sterile, spring sowing can be educated and the similar strain to F.W.V of proterties, can educate under the condition in spring sowing to get test cross F with any T-shaped sterile line test cross
1Each strain selfing gets B
3F
3
(7) select B in
3F
3Strain is divided autumn sowing, 2 processing of spring sowing, roguing and T-shaped sterile line test cross F in the simultaneously autumn sowing at varieties in Huang-Huai-Hai Plain
1, final election autumn sowing holandry is sterile, spring sowing self-fertility, with T-shaped sterile line test cross F
1There is the strain of 40～50% fertility to be the temperature sensitive non-1B/1R type K type sterile line of varieties in Huang-Huai-Hai Plain.
Spring sowing process in the said method also can be carried out in greenhouse or strange land that gamete forms phase daily mean temperature＞20 ℃.
Under the Yellow River-Huai River region ecological condition, adopt autumn sowing to cut fringe regeneration and autumn sowing, spring sowing screening, utilize in the non-1B/1R type K type male sterile line of ZL94116537.x patented technology seed selection and obtain basic temp-sensing sterile line ETS.The genetic structure characteristics of ETS material are:
Have sticking fruit goatweed (Ae.kotschyi) cytoplasm (being called for short K type cytoplasm), have spelt (T.spelta) 1B the short arm of a chromosome in the nuclear, have the main efficient recovery gene Rf of T type male sterile wheat line on this chromosome segment
3, K type sterile gene rfk
1With the fertility temperature-sensitive gene under K type cytoplasm.Rf
3Be the chromosomal marker gene of 1B, be used in the seed selection process differentiating whether choosing system belongs to non-1B/1R type.
The ETS material is done maternal and non-1B/1R type wheat hybridizing, backcross progeny all have K type cytoplasm, thereby the sterile i.e. performance of holandry has K type sterile gene rfk under the autumn sowing condition
1, spring sowing and autumn sowing are cut fringe regeneration fringe self-fertility and are shown the temperature sensitive gene of band male fertile.
The 1B/1R translocation chromosome of 1B/1R type wheat has K type sterile gene rfk
1, be maternally only to need under the spring sowing condition, to eliminate sterile strain with ETS with its hybridization, backcross progeny, the educated strain system that the proterties of selecting and remain is similar to this 1B/1R kind, warp and T type male sterile line test cross again, the non-1B/1R type of final election can be finished.
The technology path of method that 1B/1R type K type male sterile line is modified to the temperature sensitive K type of non-1B/1R type male sterile line is similar to said method, only is to reduce the hybridization workload.Hybridize, backcross with the ETS material under the spring sowing condition with this sterile line, the proterties of selecting and remain can be educated strain near breeding goal, again warp and T-shaped sterile line test cross, the non-1B/1R type of final election.
K type male sterile wheat line is meant to have the cytoplasmic wheat male sterile line of sticking fruit goatweed (Ae.kotschyi).The nuclear gene type has 3 kinds:
1. main effect sterile gene is present in wheat-rye transposition 1B/1R chromosome.
2. the K type male sterile line that inferior your remove-insurance of wild little Mace is held, major gene resistance are present in this inferior that and take off 1B chromosome.
3. utilize the good wheat nucleus genome of having of ZL94116537.x patented technology seed selection and take off the T type recovery gene Rf that 1B chromosome relates to this inferior that
3Imitate sterile gene rfk with K type master
1Fragment.
First type of effect owing to other genes on the 1R chromosome segment often produces the monoploid that frequency does not wait, and bad, Yi Sui germination, easily susceptible bring difficulty for breeding high-yield high-quality hybrid wheat.Though second class does not produce monoploid, belong to agriotype, proterties is very poor, is difficult to be used for hybrid wheat breeding.The 3rd class is transferred to good wheat cdna group with spelt 1B the short arm of a chromosome, than preceding two classes the better application prospect is arranged.
General K type sterile line need to be under the isolation condition, and to sterile line pollination production male-sterile seed, hybrid wheat generation seed is produced in sterile line and restorer hybridization by maintainer.Wheat is little strain large group crop, and the sowing line-spacing is little, manages relatively extensive.Utilization is produced hybrid wheat seed by " three are " system that sterile line, maintainer, restorer form, and in maintainer was pollinated the breeding male sterile lines production process to sterile line, because the two proterties is similar, very easily generation mixed; And two is that plant height is close, and outcrossing pollination degree is poor, protects pure difficulty, numerous kind in the numerous kind of process and yields poorly and become the key factor of restriction hybrid wheat large-scale application.
The responsive to temperature type wheat male sterile line, positive season sowing holandry is sterile to can be used for the production of hybrid seeds, and cultivation has certain selfing fertility can selfing numerous kind under temperature high ecological condition.Remove maintenance line, overcome the main mixed origin in numerous kind of process of male sterile line, simplified the hybrid wheat seed production routine.Use corresponding high-yield culturing measure, can reduce the hybrid wheat seed production cost.Hunan, the Chongqing of the China Yangtze river basin have been developed into temperature sensitive male sterile wheat line.These two kinds of temp-sensing sterile lines are grown under the warm conditions in the winter time, in the lower heading in 2～March of temperature, thereby the fertility inversion temperature is lower, through test, it is sterile that these two kinds of male sterile lines can not show holandry in the Yellow River-Huai River region autumn sowing of China's main product wheat, thereby the value of not utilizing in this zone large tracts of land.
Be used in the method for the present invention detect the T type male sterile line whether spelt 1B the short arm of a chromosome exists, be meant (T.timopheevi) the cytoplasmic common wheat male sterile line that has timopheevi wheat.The principle that detects foundation is: the recovery gene Rf of T type male sterile line
3Exist only in spelt 1B the short arm of a chromosome.Utilize the present invention with the temperature sensitive K type male sterile line that 1B/1R type wheat is modified to, under the spring sowing condition, can educate, give T type male sterile line pollination gained F
1As fertility restorer, show that then this inferior that takes off the 1B chromosome segment and exists, be non-1B/1R type.
Advantage of the present invention and good effect:
1. simplify the hybrid wheat seed production routine, improve numerous kind of benefit.The numerous kind of process of male sterile line K3314A comprises: male sterile line and maintenance line are broadcast male sterile line earlier in the alternate plantation of same isolated area, broadcast maintenance line after 10～15 days, and the florescence supplementary pollination is received male parent earlier during results, receive female parent again, general per mu yield male-sterile seed 50kg.Utilize the inventive method that the K3314A improvement is temperature sensitive non-1B/1R type K type male sterile line KTS3314A, the autumn sowing holandry is sterile, is used for the production of hybrid seeds, Yellow River-Huai River region late Febuary sowed in spring to mid-March, need not maintenance line, self-fertility, gather in the crops per mu yield male-sterile seed 200kg by the end of June.
2. Haploid production not, fertility is easily recovered.The average haplotype frequency 1～4% of K3314A.Utilize the inventive method, the temperature sensitive non-1B/1R type K type male sterile line KTS3314A of improvement, haplotype frequency is 0.F with 10 kinds difference test crosses
1Generation, higher by 17.4% than non-temperature sensitive 1B/1R class sterile line K3314A restoring degree.
3. any breed improvement can be become temperature sensitive non-1B/1R type K type male sterile line.The inventor utilized the inventive method 1B/1R type kind 3314,427-3,1376 etc. early excellent 504 with non-1B/1R type kind, plant 763,86S1002 etc. is modified to temperature sensitive non-1B/1R type K type male sterile line.
Description of drawings
Accompanying drawing is the method figure of the temperature sensitive non-1B/1R type K type male sterile line of seed selection wheat of the present invention.
Embodiment
Embodiment 1:
(1) will have the non-1B/1R type K type male sterile line KTSP3314A of spelt 1B the short arm of a chromosome in the Yellow River-Huai River region autumn sowing, a fringe bagging selfing is chosen in every strain at heading stage next year wantonly, cut off other ear stems from base portion 2～3 internodes, an amount of fertilising is poured water, and promotes to tiller regeneration; Approximately late May, regeneration is tillered the heading back by the selfing of fringe bagging, leaves and takes that the autumn sowing fringe is sterile fully, regeneration fringe self-fertility individual plant.
(2) above-mentioned each strain autumn sowing is sterile fully, the selfed seed that can educate of regeneration fringe is divided into two, a autumn sowing, another part is sowed in spring to early March by the end of February, and selection autumn sowing holandry is sterile, spring sowing self-fertility strain system, is the temperature sensitive non-1B/1RK type male sterile line ETS in basis.The autumn sowing of ETS material can be produced hybrid wheat seed with restorer hybridization, but the self propagated sterile line of sowing in spring has the advantages such as the not Haploid production of non-1B/1R type K type sterile line, easy recovery.
KTSP3314A is that He Beiru etc. utilizes the chromosome transfer method spelt 1B the short arm of a chromosome to be transferred to 1B/1R type K type male sterile line K3314A genome and the non-1B/1R type K type male sterile wheat line that obtains.This method has obtained Chinese invention patent mandate, patent No. ZL94116537.x.
Embodiment 2:
(1) take the ETS material as maternal, with non-1B/1R type wheat breed plant 763 hybridize F
1F
1Autumn sowing or spring sowing, the fertility restorer selfing obtains F
2With F
2Autumn sowing, the florescence pulls out and can educate strain, and sterile strain is appointed and is got 1 fringe bagging selfing, and selects 1～2 fringe to castrate and plant 763 and backcross, and cuts off all the other ear stems, promotes to tiller regeneration, bagging selfing before the regeneration Honoka, the autumn sowing of selecting and remain is fully sterile, the fringe of regenerating can be educated the seed B that backcrosses on the individual plant
1F
1
(2) B
1F
1Selfing obtains B
1F
2B
1F
2Autumn sowing, the florescence pulls out and can educate strain, sterile strain 1 fringe bagging selfing cuts off all the other ear stems, the sterile individual plant of regeneration fringe is pulled out in the regeneration that promotes to tiller, each fringe of regenerating can educate strain castrate and plant 763 backcross B
2F
1B
2F
1Selfing gets B
2F
2, B
2F
2Autumn sowing, the florescence pulls out and can educate strain and proterties and plant 763 dissimilar individual plants, sterile strain 1 fringe bagging selfing cuts off all the other ear stems, the regeneration that promotes to tiller, select and remain regenerate that fringe can be educated and proterties to plant 763 similar, individual plant selfing seed B
2F
3
(3) B
2F
3Menu strain selfed seed is divided into two in each, a autumn sowing, and another part sowed in spring, and the final election autumn sowing is fully sterile, spring sowing can be educated strain, and namely temperature sensitive non-1B/1R type K type is planted 763 sterile lines.
Planting 763 is wheat improved seeds of northwest wheat that plant research utilizes and the seed selection of long fringe couchgrass filial generation, belongs to non-1B/1R type, and K type male sterile line is had strong restoring force.About plant height 70cm, be spring habit, extremely early mature, product are of fine quality, comprehensive disease resistance is outstanding.
Embodiment 3:
(1) take basic temp-sensing sterile line material ETS as maternal, with 1B/1R type wheat breed 1376 hybridize F
1, F
1For autumn sowing and 1376 backcross B
1F
1
(2) B
1F
1Sow in spring (or under boot stage other ecological condition of daily mean temperature＞20 ℃, lower with), the florescence select to educate strain do maternal with 1376 backcross B
2F
1
(3) B
2F
1Sow in spring, selection traits do maternal with 1376 close educated strains and 1376 backcross B
3F
1
(4) B
3F
1Sow in spring, selection traits is similar to 1376 educates strain and pursues strain and any T-shaped sterile line test cross and selfing and get B
3F
2
(5) B
3F
2Sow in spring for roguing in each, and autumn sowing itself and T-shaped sterile line test cross F
1, the final election proterties is similar to 1376, can recover T-shaped sterile line fertility, sow in spring that to stablize the self-fertility strain be temperature sensitive non-1B/1R type K type sterile line, KTS1376.
The 1376th, Northwest Agricultural University's wheat breeding of cross breeding method seed selection.Semi-winterness, plant height 80cm, precocity, disease resistance is better, belongs to the 1B/1R type, can keep K type sterile line sterility.
Embodiment 4:
(1) 1B/1R type K type sterile line K427-3A and basic temperature sensing material ETS are sowed in spring, hybridize take K427-3A as maternal and ETS F
1
(2) under the spring sowing condition, take K427-3A as female parent and F
1Backcross B
1F
1, under the spring sowing condition, at B
1F
1The educated strain that Dai Zhongxuan proterties and 427-3 are close be male parent and K427-3A backcross B
2F
1, B
2F
1Sow in spring, select proterties similar to 427-3 educate strain do male parent and K427-3A backcross B
3F
1
(3) B
3F
1Sow in spring, select that proterties is similar to 427-3 educates strain and pursue the strain selfing, and with T-shaped sterile line test cross.
(4) B
3F
1In the menu strain and with T-shaped sterile line test cross F
1In the varieties in Huang-Huai-Hai Plain autumn sowing, the individual plant of each strain is all got 1 fringe bagging selfing before blooming, cut off all the other ear stems of tillering, promote to tiller regeneration, bagging before the regeneration Honoka, final election autumn sowing fringe is fully sterile, the regeneration fringe can be educated, and the strain that can recover T-shaped sterile line fertility be temperature sensitive non-1B/1R type K type 427-3 sterile line.
K427-3A is the 1B/1R type K type male sterile line that semidominant causes short ability that has of Northwest Agricultural University's seed selection.Winter habit, plant height 55cm, mid-early maturity, resistance are better.
Claims (1)
1. a seed selection adapts to the method for Chinese Yellow River-Huai River region wheat responsive to temperature male sterile line, it is characterized in that utilizing the non-1B/1R type K type male sterile line of existing method seed selection, therefrom select basic thermo-sensitive sterile material ETS, thermo-sensitive sterile material ETS is sterile in Yellow River-Huai River region autumn sowing holandry, the spring sowing self-fertility; ETS becomes this kind temp-sensing sterile line with arbitrarily small wheat variety by the method transformation of backcrossing, and its selection comprises the steps:
One, seed selection basis thermo-sensitive sterile material ETS:(1) will have the non-1B/1R type K type male sterile wheat line of spelt 1B the short arm of a chromosome at the Yellow River and Huai He River winter wheat district planted in fall, the every strain cover 1-2 fringe selfing of heading back, all the other tillering tassels cut off from base portion 2～3 internodes; (2) regeneration is tillered heading back by the selfing of fringe bagging, ripe before by strain results self-fertility seed; (3) with autumn sowing sterile and regeneration fringe can educate single-strain seed in the Yellow River and Huai He River winter wheat district by strain autumn sowing and spring sowing, the strain system that autumn sowing is sterile fully, the spring sowing selfing can be solid of selecting and remain is basic temperature sensitive non-1B/1R type K type male sterile line ETS;
Two, hybridize take ETS as maternal and the good wheat breed F.W.V. of non-1B/1R type F
1Generation, F
1Get F for selfing
2Generation, F
2For autumn sowing, select the selfing of 1-2 fringe bagging heading stage, and backcross with F.W.V. with 1-2 fringe work is maternal, cut off all the other ear stems, promote to tiller regeneration, the selfing of regeneration fringe bagging, the autumn sowing fringe of the selecting and remain seed that backcrosses fully sterile, that the fringe of regenerating can be educated on the individual plant gets B
1F
1Generation, B
1F
1Get B for selfing
1F
2Generation, B
1F
2In generation, is to B
3F
2In generation, selected all as stated above and backcrosses B
3F
2Proterties is similar to F.W.V., autumn sowing fringe holandry is sterile for selecting and remain under the autumn sowing condition, the regeneration fringe can be educated the selfed seed B of individual plant
3F
3, B
3F
3Each individual plant divides autumn sowing, two processing of sowing in spring, and selection traits is similar to F.W.V., autumn sowing is fully sterile, spring sowing can be educated strain and be got B
3F
4, be the temp-sensing sterile line of this non-1B/1R type F.W.V.;
Three, hybridize take ETS as maternal and 1B/1R improved seeds F.W.V. F
1Generation, F
1Sowing in spring in generation, work is maternal to be backcrossed with F.W.V., and continuous backcross is to B under the spring sowing condition
3F
1Generation, B
3F
2, B
3F
3Processing method is identical with the generation material with step 2, for guaranteeing that the temp-sensing sterile line that obtains is non-1B/1R type, at B
3F
2Or B
3F
3In generation, selected target individual plant or strain and T-shaped sterile line test cross, test cross F
1F is selected in autumn sowing
1The test cross male parent strain that fertility is restored namely obtains the temp-sensing sterile line of this kind;
Four, under the spring sowing condition, with 1B/1R type K type sterile line KF.W.V.A do maternal and ETS hybridize F
1Generation, and the educated strain similar to F.W.V. of Continuous Selection proterties made male parent and KF.W.V.A backcrossed for 3 generations to B under the spring sowing condition
3F
1Generation, B
3F
1In generation, sow in spring, and the proterties of the selecting and remain fertile plant selfing similar to F.W.V. gets B
3F
2Generation, B
3F
2In generation and later on respectively identical with step 3 for processing method, the transformation of 1B/1R type K type sterile line can be become non-1B/1R type temp-sensing sterile line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001054880A CN1141017C (en) | 2000-04-03 | 2000-04-03 | Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001054880A CN1141017C (en) | 2000-04-03 | 2000-04-03 | Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1316182A CN1316182A (en) | 2001-10-10 |
| CN1141017C true CN1141017C (en) | 2004-03-10 |
Family
ID=4577735
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001054880A Expired - Fee Related CN1141017C (en) | 2000-04-03 | 2000-04-03 | Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1141017C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105075851B (en) * | 2015-08-25 | 2018-03-06 | 江苏丘陵地区镇江农业科学研究所 | A kind of wheat temperature Photosensitive sterile line transfer method |
| CN112740967A (en) * | 2021-01-29 | 2021-05-04 | 新疆农垦科学院 | Seed production method for three-line hybrid wheat precision hole sowing |
-
2000
- 2000-04-03 CN CNB001054880A patent/CN1141017C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1316182A (en) | 2001-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102696474B (en) | Breeding and application of cytoplasmic male sterility restoring line of brassica napus rapeseed and radish | |
| JP2018533961A (en) | Polyploid 2 hybrid rice and its breeding method | |
| CN104719129A (en) | Breeding method of novel wheat variety | |
| CN102217526B (en) | A kind of selection of black barley | |
| Li et al. | Hybridization between Triticum aestivum L. and Agropyron michnoi Roshev. 1. Production and cytogenetic study of F 1 hybrids | |
| CN100397977C (en) | Breed method for rice reproduction heterosis fixation | |
| CN105409762A (en) | Cyclic breeding method of cotton varieties | |
| CN109042292A (en) | A kind of hybrid rice seed breeding process of same period rice transplanting parent | |
| Muminov et al. | Analysis of yield and fiber quality traits in intraspecific and interspecific hybrids of cotton | |
| CN102805029A (en) | Cultivation method of hybrid rapeseed capable of being planted at high altitude localities | |
| CN110122315B (en) | Method for breeding multi-series hybrid variety resisting rice blast by using rice temperature-sensitive permanent genic male sterile line | |
| CN1813523A (en) | Wheat interspecific distant hybrid, secondary backcross and selfing breeding method | |
| CN103348908B (en) | Method for selecting hybrid seeds of Brassica juncea var. multiceps | |
| CN1895030A (en) | Breeding method of Chinese cabbage hybrid | |
| CN102100175A (en) | Breeding and hybrid seed-producing method of onion male sterile line | |
| CN1141017C (en) | Method for selectively breeding temperature-sensitive sterile line of wheat suitable for Yellow River-Huai River region in China | |
| CN1895029A (en) | Breeding method of Chinese cabbage cytoplasmic male sterile line germplasm material | |
| CN1307866C (en) | Rape cytoplasmic male sterility + self incompatibility hybrid superiority using method | |
| CN1144516C (en) | Production method of hybrid wheat seeds | |
| CN108419670A (en) | A kind of long fringe neck novel rice sterile line breeding method | |
| CN108990794B (en) | Hybrid rice breeding method | |
| CN100401878C (en) | Method for improving breeding benefit of three-line hybrid wheat | |
| CN101356894B (en) | Method for breeding and using sporophyte type rice sterile line | |
| CN101326897B (en) | Method for breeding celery cabbage temperature-sensitive male sterile line and temperature-insensitive male sterile line | |
| CN1064812C (en) | Tech. for breeding K type hybridized wheat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |