CN114097546A - Green planting and fertilizing method for leaf vegetable crops - Google Patents

Green planting and fertilizing method for leaf vegetable crops Download PDF

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CN114097546A
CN114097546A CN202010898502.6A CN202010898502A CN114097546A CN 114097546 A CN114097546 A CN 114097546A CN 202010898502 A CN202010898502 A CN 202010898502A CN 114097546 A CN114097546 A CN 114097546A
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powder
compound microbial
soil
stubble
fertilizer
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CN114097546B (en
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陈露
闫龙翔
金明弟
李建勇
阚雨晨
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Shanghai Lvle Bio Tech Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to a green planting and fertilizing method of leaf vegetable crops, which comprises the following steps: (1) taking the compound microbial fertilizer and the soil conditioning microbial agent as base fertilizers, mixing or respectively and independently applying the base fertilizers to vegetable field obstacle soil, directly sowing or transplanting the base fertilizers after application, and keeping the soil moist; spreading the composite microbial fertilizer according to the using amount of 30-150 kg/mu & stubble; the soil conditioning microbial agent is spread according to the dosage of 60-80 kg/mu & stubble; (2) topdressing a compound microbial fertilizer and a compound microbial agent when seedlings are lifted or live, wherein the compound microbial fertilizer is applied at 25-50 kg/mu.stubble, and watering is needed after spreading; after being mixed with water, the compound microbial agent is sprayed, flushed or irrigated to roots of the leaf vegetable crops according to the dosage of 10-20 kg/mu & stubble; the nutrient content in the compound microbial fertilizer is 5-30 wt%. The crops produced by the method accord with the standard of green vegetables and have the effect of increasing yield.

Description

Green planting and fertilizing method for leaf vegetable crops
Technical Field
The invention relates to the technical field of crop fertilization, in particular to a green planting and fertilizing method for leaf vegetable crops.
Background
The leaf vegetable crops include pakchoi, celery, spinach and lettuce which are eaten with tender leaves and stems, common head cabbage and Chinese cabbage which are eaten with leaf balls, and onion, green onion, garlic and the like which are eaten with tender bulbs. The leaf vegetable crops absorb nutrients and are characterized in that: the absorption of three-element nutrients still takes potassium as the highest, but compared with fruits and vegetables, the absorbed potassium and nitrogen amount per 100 kg of yield is 2:1 for the fruits and vegetables, and the absorbed potassium and nitrogen amount per 100 kg of yield is close to 1:1 for the leaves and vegetables. This is mainly due to the relatively high amount of nitrogen absorbed by leaf vegetables, not to the low potassium absorption. Secondly, the root system of the leaf vegetable crops is buried shallowly, belongs to shallow root crops, and has lower drought resistance and waterlogging resistance. When the soil is too wet and the nutrient content is too low, the absorption of the soil nutrient can be seriously influenced; when the soil is dry, the crops are easy to have the symptoms of calcium deficiency and boron deficiency. ③ the nutrient elements in the plants of the leaf vegetables are continuously accumulated in the whole growth period, but the nutrient absorption speed is in the early period of the growth when the peak is high. The nutrient absorption of the crops in the later growth period is relatively low, so that the nutrients of the crops in the early growth period have a large relationship with the whole growth period, and have important effects on yield and quality.
Because leaf vegetables have great requirements on nitrogen and potassium, farmers mainly use nitrogen and phosphorus fertilizers as main fertilizers in the practical production, and the input amount of potassium fertilizers is small. And the long-term application of nitrogen and phosphorus fertilizers causes unbalanced nutrient supply of vegetable field soil, which is mainly characterized in that the content of effective nitrogen and phosphorus in the soil is increased, the content of effective potassium is reduced, and the soil nitrate is accumulated to generate secondary salinization, so that the productivity is reduced.
Disclosure of Invention
In order to solve the technical problems of unbalanced vegetable field soil nutrients and productivity reduction caused by secondary salinization of soil due to long-term partial application of nitrogen and phosphorus fertilizers in actual production, a green planting and fertilizing method for leaf vegetable crops is provided. The method can effectively solve the problem of unbalanced soil nutrients and the problem of secondary salinization of soil without using conventional fertilizers, and can effectively promote the yield increase of leaf vegetable crops.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the green planting and fertilizing method of the leaf vegetable crops comprises the following steps:
(1) taking a compound microbial fertilizer and a soil conditioning microbial agent as base fertilizers, mixing or respectively and independently applying the base fertilizers to vegetable field obstacle soil, directly sowing seeds of leaf vegetable crops or transplanting seedlings of the leaf vegetable crops after applying, and keeping the soil moist after sowing or transplanting;
the compound microbial fertilizer is spread according to the use amount of 30-150 kg/mu & stubble;
the soil conditioning microbial agent is spread according to the dosage of 60-80 kg/mu & stubble;
(2) topdressing a compound microbial fertilizer and a compound microbial agent when seedlings are lifted or live, wherein the compound microbial fertilizer is applied at a rate of 25-50 kg/mu.stubble, and watering is needed after spreading; after being mixed with water, the compound microbial agent is sprayed, flushed or irrigated to roots of the leaf vegetable crops according to the dosage of 10-20 kg/mu & stubble;
the nutrient content in the compound microbial fertilizer is 5-30 wt%.
Further, in the base fertilizer in the step (1), the compound microbial fertilizer with 5-10 wt% of nutrient content and the compound microbial fertilizer with 20-30 wt% of nutrient content are applied according to the use amounts of 60-150 kg/mu and 30-60 kg/mu, and are applied once per crop.
Preferably, in the base fertilizer in the step (1), the compound microbial fertilizer with the nutrient content of 8 wt% and the compound microbial fertilizer with the nutrient content of 25 wt% are respectively applied according to the usage amounts of 80 kg/mu and 40 kg/mu. The compound microbial fertilizer with the nutrient content of 8 wt% and the compound microbial fertilizer with the nutrient content of 25 wt% are compounded, so that leaf vegetable crops can reach the green standard of vegetables without increasing the production cost. If not compounded, the compound microbial fertilizer with 25 wt% of nutrient content is adopted, although the using amount is less and the application is more convenient for farmers, the produced leaf vegetable crops do not meet the green standard; if the compound microbial fertilizer with the nutrient content of 8 wt% is adopted, the application amount is increased, and further, the labor cost and the production cost of farmers are increased.
Further, in the additional fertilizer in the step (2), the compound microbial fertilizer with 20 wt% of nutrient content is spread according to the use amount of 30-35 kg/mu.stubble.
Further, the compound microbial fertilizer comprises 5-30 wt% of nutrients, 0.2-2 wt% of microbial powder, 1 wt% of aspergillus oryzae bacterial powder, 0.5-3 wt% of trace element source and the balance of oil soil; the effective viable count of the compound microbial fertilizer is more than 0.5 hundred million/g; the nutrient comprises a nitrogen source, a phosphorus source and a potassium source, and the mass ratio of the nitrogen source to the phosphorus source to the potassium source is (10-15) to (4-6) to (6-10). Sufficient nitrogen supply of leaf vegetables is particularly important, and if the nitrogen supply is insufficient, plants are short and small, leaves are small, leaves at stem bases are easy to wither, yellow and fall off, and tissues are rough and hard.
Still further, the microbial powder comprises bacillus megaterium powder.
Still further, the microbial powder also comprises bacillus mucilaginosus powder or bacillus subtilis powder.
Further, the soil conditioning microbial agent comprises humic acid and bacillus pumilus fermentation liquor; the mass ratio of the Bacillus pumilus fermentation liquor to the humic acid is (8.5-9) to 70; the Bacillus pumilus fermentation liquid is prepared by placing Bacillus pumilus powder at pH of 7.2, wherein the pH is 1g/L of corn starch and 10g/L, MgSO of yeast extract44.9g/L of mixed aqueous solution is obtained by fermenting for 10 hours at the temperature of 37 ℃, and the adding amount of the bacillus pumilus powder in the mixed aqueous solution is 0.03-0.04 wt%. The fermentation liquid after the fermentation of the bacillus pumilus can effectively relieve secondary salinization of soil, and nitrate nitrogen is converted into organic nitrogen for being absorbed and utilized by crops.
Still further, the soil conditioning microbial agent also comprises a paenibacillus polymyxa fermentation liquor, and the mass ratio of the paenibacillus polymyxa fermentation liquor to the humic acid is (1-1.5): 70; the fermentation liquid of the paenibacillus polymyxa is prepared by placing paenibacillus polymyxa powder into 44.30g of cane sugar with the initial pH value of 7/L、(NH4)2SO4 6.756g/L、CaCO36.32g/L、MgSO4 0.25g/L、KH2PO40.22g/L, NaCl 0.18.18 g/L of mixed aqueous solution is obtained by fermenting for 60 hours at the temperature of 37 ℃, and the addition amount of the paenibacillus polymyxa powder in the mixed aqueous solution is 0.025wt percent to 0.035wt percent. The fermentation liquor of the paenibacillus polymyxa contains various metabolites, and has good prevention and treatment effects on obstacle soil, particularly various bacterial diseases and fungal soil-borne diseases.
Further, the compound microbial agent is prepared from bacillus subtilis powder and bacillus mucilaginosus powder according to the mass ratio of 9: 1; the composite microbial agent is blended with water according to the mass ratio of 1 (5-10), and the effective viable count after blending with water is at least 5 hundred million/mL. Can help the seedling growth of leaf vegetables and promote the absorption of nutrients by the leaf vegetables.
The beneficial technical effects are as follows:
the method changes a fertilization mode taking crop yield as a center and large fertilizer input as means into a green planting fertilization method taking quality of leaf vegetable agricultural products as a center, green production requirements as a guide and production data input of the green leaf vegetable agricultural products as means, is more accurate and more effective in fertilization, and avoids adverse effects on environment and safety of the leaf vegetable agricultural products caused by blind fertilization; the compound microbial fertilizer with the nutrient content of 8 wt% and the compound microbial fertilizer with the nutrient content of 25 wt% are compounded to serve as base fertilizers to be applied to vegetable field obstacle soil, then the compound microbial fertilizer is matched with a soil conditioning microbial agent to improve secondary salinization obstacle soil of the vegetable field, the compound microbial fertilizer is applied during topdressing, and the compound microbial agent is sprayed, flushed or irrigated with water during seedling raising or seedling survival, so that the problem of unbalanced soil nutrients and the problems of secondary salinization and soil-borne diseases of the soil can be effectively improved, leaf vegetables can reach the standard of green vegetables without increasing the production cost, and the yield of the leaf vegetables can be effectively increased.
The compound microbial fertilizer contains a large amount of beneficial microorganisms, is compounded with high-quality organic matters, and is compounded by major elements and trace elements, and has the characteristics of supplementing soil nutrients, preventing nutrient imbalance, increasing the number of beneficial microorganisms in soil, inhibiting soil-borne diseases, increasing the content of organic matters in soil and increasing the soil fertility.
The compound microbial agent can help the seedling growth of leaf vegetable crops, promote the absorption of nutrients by the leaf vegetables, effectively inhibit the growth of harmful microorganisms through mass propagation after being applied to soil, reduce the occurrence of soil-borne diseases and improve the soil micro-ecological environment.
The microecological flora of vegetable field obstacle soil can be reconstructed by the fertilization method, the application of the conventional organic fertilizer is replaced, the pollution to underground water and agricultural products is reduced, the vegetable field soil is improved, the quality of leaf vegetable crops is improved, meanwhile, the diseases of the leaf vegetable crops are effectively inhibited, and the yield is increased.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Unless specifically stated otherwise, the numerical values set forth in these examples do not limit the scope of the invention. Techniques, methods known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values.
The following examples used Bacillus megaterium powder (more than 100 hundred million/g effective viable bacteria), Bacillus mucilaginosus powder (more than 50 hundred million/g effective viable bacteria), Bacillus subtilis powder (more than 800 hundred million/g effective viable bacteria), Bacillus pumilus (more than 900 hundred million/g effective viable bacteria), and Paenibacillus polymyxa (more than 50 hundred million/g effective viable bacteria) all obtained from Jinyang Biotechnology Co., Ltd, Aspergillus oryzae powder (more than 50 hundred million/g effective viable bacteria) obtained from Suislandia biological GmbH, the used oil soil is organic carbon filter cake produced in edible oil production, specifically soybean oil organic carbon filter cake raw material, and the basic properties refer to the data in Table 1 of Chinese patent 201810924416.0.
Example 1
The green planting and fertilizing method of the leaf vegetable crops comprises the following steps:
(1) taking a compound microbial fertilizer and a soil conditioning microbial agent as base fertilizers, uniformly mixing, spreading the base fertilizers on vegetable field obstacle soil, ploughing after spreading, sowing seeds of leaf vegetable crops, and keeping the soil moist after sowing;
(2) the compound microbial fertilizer is firstly spread for topdressing in the growth period of leaf vegetable crops, and watering is needed after spreading; applying a compound microbial agent during seedling lifting or survival, and spraying, flushing or irrigating roots of the leaf vegetable crops after the compound microbial agent is added with water;
the specific process was carried out according to the method in table 1.
Table 1 fertilization method for green planting of example 1
Figure BDA0002659128300000051
The compound microbial fertilizer used in table 1 of this example is in solid state, and may be in granule or powder form.
The compound microbial fertilizer comprises the following components: 8 wt% of nutrient (including urea, monoammonium phosphate and potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 10:6:10), 1 wt% of bacillus megaterium powder, 1 wt% of aspergillus oryzae powder, 1 wt% of trace element source (ferrous sulfate, zinc sulfate and borax in equal mass ratio), and the balance of oil soil.
The second composite microbial fertilizer consists of: 25 wt% of nutrient (including urea, monoammonium phosphate and potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 10:6:10), 1 wt% of bacillus megaterium powder, 1 wt% of aspergillus oryzae powder, 1 wt% of trace element source (ferrous sulfate, zinc sulfate and borax in equal mass ratio), and the balance of oil soil.
The compound microbial fertilizer comprises the following components: 20 wt% of nutrient (comprising urea, monoammonium phosphate and potassium nitrate in a mass ratio of 10:6:10), 1 wt% of bacillus megaterium powder, 1 wt% of aspergillus oryzae powder, 1 wt% of trace element source (ferrous sulfate, zinc sulfate and borax in equal mass ratio), and the balance of oil soil.
The effective viable count of the bacillus megaterium powder in the composite microbial fertilizer is more than 100 hundred million/g; the effective viable count of the Aspergillus oryzae strain powder is more than 50 hundred million/g. The effective viable count of the compound microbial fertilizer is actually measured to be more than 1 hundred million/g.
The soil conditioning microbial agent comprises humic acid and bacillus pumilus fermentation liquor; the mass ratio of the bacillus pumilus fermentation liquor to the humic acid is 8.75: 70; the Bacillus pumilus fermentation liquid is prepared by placing Bacillus pumilus powder at pH of 7.2, wherein the pH is 1g/L of corn starch and 10g/L, MgSO of yeast extract4Fermenting 4.9g/L mixed water solution at 37 deg.C for 10 hr, and adding 0.035g Bacillus pumilus powder into 100g mixed water solution. The fermentation liquid after the fermentation of the bacillus pumilus can effectively relieve secondary salinization of soil, and nitrate nitrogen is converted into organic nitrogen for being absorbed and utilized by crops.
The compound microbial agent is prepared by bacillus subtilis powder and bacillus mucilaginosus powder according to the mass ratio of 9: 1; the composite microbial agent is prepared by blending with water according to the mass ratio of 1:8, and the effective viable count after blending with water is at least 5 hundred million/mL.
The embodiment is mainly directed to vegetable field obstacle soil with a secondary salinization problem.
Example 2
The present embodiment is the same as the fertilizing method of embodiment 1, except that:
the specific process was carried out according to the method in table 2.
Table 2 fertilization method for green planting of example 2
Figure BDA0002659128300000061
The compound microbial fertilizer comprises the following components: 5 wt% of nutrient (comprising urea, monoammonium phosphate such as potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 13:5:8), 1 wt% of bacillus megaterium powder, 0.5 wt% of colloidal bacillus powder, 1 wt% of aspergillus oryzae powder, 0.5 wt% of trace element source (ferrous sulfate, zinc sulfate and borax according to equal mass ratio), and the balance of oil soil.
The second composite microbial fertilizer consists of: 30 wt% of nutrient (comprising urea, monoammonium phosphate such as potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 13:5:8), 1 wt% of bacillus megaterium powder, 0.5 wt% of colloidal bacillus powder, 1 wt% of aspergillus oryzae powder, 0.5 wt% of trace element source (ferrous sulfate, zinc sulfate and borax according to equal mass ratio), and the balance of oil soil.
The compound microbial fertilizer comprises the following components: 20 wt% of nutrient (comprising urea, monoammonium phosphate such as potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 13:5:8), 1 wt% of bacillus megaterium powder, 0.5 wt% of colloidal bacillus powder, 1 wt% of aspergillus oryzae powder, 0.5 wt% of trace element source (ferrous sulfate, zinc sulfate and borax according to equal mass ratio), and the balance of oil soil.
The effective viable count of the bacillus megaterium powder in the composite microbial fertilizer is more than 100 hundred million/g; the effective viable count of the Aspergillus oryzae strain powder is more than 50 hundred million/g; the effective viable count of the bacillus mucilaginosus powder is more than 80 hundred million/g. The effective viable count of the compound microbial fertilizer is actually measured to be more than 1.5 hundred million/g.
The soil conditioning microbial agent comprises humic acid, a bacillus pumilus fermentation liquor and a paenibacillus polymyxa fermentation liquor, the mass ratio of the paenibacillus polymyxa fermentation liquor to the humic acid is 1.25:70, and the paenibacillus polymyxa fermentation liquor is prepared by placing a paenibacillus polymyxa strain in 44.30g/L (NH) containing cane sugar with the initial pH value of 74)2SO4 6.756g/L、CaCO3 6.32g/L、MgSO4 0.25g/L、KH2PO4Fermenting 0.22g/L NaCl0.18g/L mixed aqueous solution at 37 ℃ for 60h, and adding 0.03g of paenibacillus polymyxa powder into each 100g of the mixed aqueous solution. The fermentation liquor of the paenibacillus polymyxa contains various metabolites, and has good prevention and treatment effects on obstacle soil, particularly various bacterial diseases and fungal soil-borne diseases.
The compound microbial agent is prepared by bacillus subtilis powder and bacillus mucilaginosus powder according to the mass ratio of 9: 1; the composite microbial agent is prepared by blending with water according to the mass ratio of 1:5, and the effective viable count after blending with water is at least 5 hundred million/mL.
The embodiment is mainly directed to vegetable field obstacle soil with secondary salinization problems and soil-borne diseases.
Example 3
The present embodiment is the same as the fertilizing method of embodiment 1, except that:
the specific procedure was carried out according to the method in table 3.
Table 3 fertilization method for green planting of example 3
Figure BDA0002659128300000071
The compound microbial fertilizer comprises the following components: 10 wt% of nutrient (comprising urea, monoammonium phosphate such as potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 15:4:6), 1 wt% of bacillus megaterium powder, 0.05 wt% of bacillus subtilis powder, 1 wt% of aspergillus oryzae powder, 1.5 wt% of trace element source (ferrous sulfate, zinc sulfate and borax according to equal mass ratio), and the balance of oil soil.
The second composite microbial fertilizer consists of: 20 wt% of nutrient (comprising urea, monoammonium phosphate such as potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 15:4:6), 1 wt% of bacillus megaterium powder, 0.05 wt% of bacillus subtilis powder, 1 wt% of aspergillus oryzae powder, 1.5 wt% of trace element source (ferrous sulfate, zinc sulfate and borax according to equal mass ratio), and the balance of oil soil.
The compound microbial fertilizer comprises the following components: 20 wt% of nutrient (comprising urea, monoammonium phosphate such as potassium nitrate, the mass ratio of the urea to the monoammonium phosphate to the potassium nitrate is 15:4:6), 1 wt% of bacillus megaterium powder, 0.05 wt% of bacillus subtilis powder, 1 wt% of aspergillus oryzae powder, 1.5 wt% of trace element source (ferrous sulfate, zinc sulfate and borax according to equal mass ratio), and the balance of oil soil.
The effective viable count of the bacillus megaterium powder in the composite microbial fertilizer is more than 100 hundred million/g; the effective viable count of the Aspergillus oryzae strain powder is more than 50 hundred million/g; the effective viable count of the bacillus subtilis powder is more than 1000 hundred million/g. The effective viable count of the compound microbial fertilizer is actually measured to be more than 1.5 hundred million/g.
The soil conditioning microbial inoculum used was the same as in example 1.
The compound microbial agent is prepared by bacillus subtilis powder and bacillus mucilaginosus powder according to the mass ratio of 9: 1; the composite microbial agent is prepared by blending with water according to the mass ratio of 1:10, and the effective viable count after blending with water is at least 5 hundred million/mL.
The embodiment is mainly directed to vegetable field obstacle soil with a secondary salinization problem.
In the above examples, if the soil conditioner is not a soil obstacle but a general soil, the soil conditioner microbial agent does not need to be applied.
Comparative example 1
This comparative example is the same as the fertilization procedure of example 1, except that: the base fertilizer completely adopts composite microbial fertilizer (8 wt% of nutrient content), and the dosage is 180 kg/mu.stubble.
Comparative example 2
This comparative example is the same as the fertilization procedure of example 1, except that: the base fertilizer is prepared from compound microbial fertilizer (25 wt% of nutrient content) in an amount of 80 kg/mu.
Comparative example 3
This comparative example is the same as the fertilization procedure of example 1, except that: no compound microbial fertilizer (20 wt% of nutrient content) is applied in the top dressing.
Comparative example 4
This comparative example is the same as the fertilization procedure of example 1, except that: the compound microbial inoculum is not applied in the top dressing.
Example 4
Field test of pakchoi
Test work: rape seed; variety: shanghai Qing; test site: shanghai Fuxi vegetable and fruit professional cooperative (Fuxi for short).
The fertilizer application materials used in the tests were the compound microbial fertilizer (8 wt% nutrient content), the compound microbial fertilizer (25 wt% nutrient content), the compound microbial fertilizer (20 wt% nutrient content), the soil conditioning agent and the compound microbial agent prepared in examples 1 to 3, respectively, and were all prepared by Shanghai Lvle Biotech Limited.
Eight treatments are set in the test, the four times of treatment are repeated, the planting density is 5000 plants/mu, the plants are randomly arranged in blocks, and the area of each cell is 30m2
Eight tests are set at each site, and the contents of each test are as follows:
test T1: the fertilization procedure is as in Table 1 of example 1;
test T2: the fertilization procedure is as in Table 2 of example 2;
test T3: the fertilization procedure is as in Table 3 of example 3;
test T4: fertilizing method according to comparative example 1;
test T5: fertilizing method according to comparative example 2;
test T6: fertilizing method according to comparative example 3;
test T7: the fertilization procedure of comparative example 4 was followed.
Test T8: applying fertilizer according to the conventional method and applying according to local habits.
Habitual fertilization: 15-15-15 kg of base fertilizer and 30 kg/mu of compound fertilizer, and 15-20kg of additional fertilizer.
The basic agronomic traits of the tested soils are shown in table 4.
TABLE 4 basic agro-chemical traits of the soil tested
Figure BDA0002659128300000091
The results of the eight tests on the agronomic traits of pakchoi are shown in table 5.
TABLE 5 Effect of different tests on the agronomic traits of Brassica rapa
Test of Plant height/cm Number of blades/sheet Weight per gram of individual plant
T 1 18.5 6.8 36.6
T 2 18.2 6.3 35.1
T 3 18.1 6.5 36.2
T 4 18.3 6.4 35.9
T 5 17.3 5.7 34.0
T 6 16.8 5.6 28.2
T 7 16.4 5.1 27.7
T 8 18.0 6.2 29.8
The results of the eight tests on the yield of pakchoi are shown in table 6.
TABLE 6 Effect of different experiments on the yield of Brassica rapa
Figure BDA0002659128300000092
Figure BDA0002659128300000101
As can be seen from tables 5 and 6, comparing T1, T2, T3 and T8, after the pakchoi is fertilized and planted according to the fertilization methods of the embodiments 1, 2 and 3 of the present invention, no additional chemical fertilizer is required to be added, and the agronomic characters of the pakchoi are much better than the agronomic shapes of the pakchoi fertilized conventionally; compared with T8, the average yield of T1, T2, T3 is increased by more than 13.5 percent compared with that of T8, and all the average yields meet the green vegetable standard.
Comparing T1 with T4 and T5, the base fertilizer in T4 is a compound microbial fertilizer which uses 8 wt% of nutrient content, although the finally obtained pakchoi can reach the standard of green vegetables, the yield increase rate is equivalent to that of the T8 in the conventional fertilization method, but the application amount of the compound microbial fertilizer which adopts 8 wt% of nutrient content and 25 wt% of nutrient content in T1 is increased, so that the labor cost and the production cost of farmers are increased; compared with the compound microbial fertilizer prepared in the step T1, the compound microbial fertilizer prepared in the step T5 totally adopts 25 wt% of nutrient content, the agricultural property of the pakchoi is poor, the yield is increased although the yield is increased, and the yield of the pakchoi is slightly lower than that of the pakchoi prepared by the method, and the produced pakchoi does not meet the standard of green vegetables.
Comparing T1 with T6 and T7, it can be seen that compound microbial fertilizer (20 wt% of nutrient content) is not applied in the top dressing of T6, and compound microbial inoculum is not applied in the top dressing of T7, although the produced pakchoi meets the standard of green vegetables, the agronomic characters of pakchoi are all worse than that of T1 of the invention, and the yield is slightly reduced.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (10)

1. The green planting and fertilizing method of the leaf vegetable crops is characterized by comprising the following steps:
(1) taking a compound microbial fertilizer and a soil conditioning microbial agent as base fertilizers, mixing or respectively and independently applying the base fertilizers to vegetable field obstacle soil, directly sowing seeds of leaf vegetable crops or transplanting seedlings of the leaf vegetable crops after applying, and keeping the soil moist after sowing or transplanting;
the compound microbial fertilizer is spread according to the use amount of 30-150 kg/mu & stubble;
the soil conditioning microbial agent is spread according to the dosage of 60-80 kg/mu & stubble;
(2) topdressing a compound microbial fertilizer and a compound microbial agent when seedlings are lifted or live, wherein the compound microbial fertilizer is applied at a rate of 25-50 kg/mu.stubble, and watering is needed after spreading; after being mixed with water, the compound microbial agent is sprayed, flushed or irrigated to roots of the leaf vegetable crops according to the dosage of 10-20 kg/mu & stubble;
the nutrient content in the compound microbial fertilizer is 5-30 wt%.
2. The method for green planting and fertilizing of leaf vegetable crops as claimed in claim 1, characterized in that in step (1), the compound microbial fertilizer with 5-10 wt% of nutrients and 20-30 wt% of nutrients in the base fertilizer is spread according to the dosage of 60-150 kg/mu-stubble and 30-60 kg/mu-stubble, and is applied once per stubble.
3. The method for green planting and fertilizing of leaf vegetable crops as claimed in claim 2, characterized in that in step (1), the compound microbial fertilizer with nutrient content of 8 wt% and the compound microbial fertilizer with nutrient content of 25 wt% are respectively applied according to the dosage of 80 kg/mu.s.stubble and 40 kg/mu.s.stubble.
4. The method for green planting and fertilizing of leaf vegetables as claimed in claim 1, wherein in the step (2), the compound microbial fertilizer with 20 wt% of nutrient content is applied according to the dosage of 30-35 kg/mu-stubble.
5. The green planting and fertilizing method for leaf vegetables as claimed in claim 1, wherein the compound microbial fertilizer comprises 5-30 wt% of nutrients, 0.2-2 wt% of microbial powder, 1 wt% of aspergillus oryzae bacterial powder, 0.5-3 wt% of trace element source, and the balance of oil soil; the effective viable count of the compound microbial fertilizer is more than 0.5 hundred million/g; the nutrient comprises a nitrogen source, a phosphorus source and a potassium source, and the mass ratio of the nitrogen source to the phosphorus source to the potassium source is (10-15) to (4-6) to (6-10).
6. The method for green planting and fertilizing of leaf vegetable crops as claimed in claim 5, characterized in that the microbial powder comprises Bacillus megaterium powder.
7. The method for green planting and fertilizing of leaf vegetables according to claim 6, wherein the microbial powder further comprises Bacillus mucilaginosus powder or Bacillus subtilis powder.
8. The method for green planting and fertilizing of leaf vegetable crops as claimed in claim 1, characterized in that the soil conditioning microbial agent comprises humic acid and bacillus pumilus fermentation liquor; the mass ratio of the Bacillus pumilus fermentation liquor to the humic acid is (8.5-9) to 70; the Bacillus pumilus fermentation liquid is prepared by placing Bacillus pumilus powder at pH of 7.2, wherein the pH is 1g/L of corn starch and 10g/L, MgSO of yeast extract44.9g/L of mixed aqueous solution is obtained by fermenting for 10 hours at the temperature of 37 ℃, and the adding amount of the bacillus pumilus powder in the mixed aqueous solution is 0.03-0.04 wt%.
9. The green planting and fertilizing method for leaf vegetable crops as claimed in claim 8, characterized in that the soil conditioning microbial agent further comprises a Paenibacillus polymyxa fermentation liquor, the mass ratio of the Paenibacillus polymyxa fermentation liquor to the humic acid is (1-1.5): 70; the fermentation liquid of the paenibacillus polymyxa is prepared by placing paenibacillus polymyxa powder into 44.30g/L (NH) containing cane sugar with the initial pH value of 74)2SO4 6.756g/L、CaCO3 6.32g/L、MgSO4 0.25g/L、KH2PO40.22g/L, NaCl 0.18.18 g/L of mixed aqueous solution is obtained by fermenting for 60 hours at the temperature of 37 ℃, and the addition amount of the paenibacillus polymyxa powder in the mixed aqueous solution is 0.025wt percent to 0.035wt percent.
10. The green planting and fertilizing method for leaf vegetables according to claim 1, characterized in that the compound microbial agent is prepared from bacillus subtilis powder and bacillus mucilaginosus powder according to a mass ratio of 9: 1; the composite microbial agent is blended with water according to the mass ratio of 1 (5-10), and the effective viable count after blending with water is at least 5 hundred million/mL.
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