Disclosure of Invention
The invention aims to solve the defects that a test tube is easy to drop and the extraction efficiency is low in the prior art, and provides an extraction device for synthesizing cell-free protein.
In order to achieve the purpose, the invention adopts the following technical scheme:
an extraction device for synthesizing cell-free protein comprises a bottom box, wherein a bearing plate is slidably arranged in the bottom box, an accommodating cavity is formed in the bearing plate, a motor is fixedly arranged in the accommodating cavity, a rotating rod is fixedly arranged on an output shaft of the motor, a first gear is fixedly arranged on the rotating rod, a double-sided rack is meshed on the first gear, a second gear is rotatably arranged in the accommodating cavity and meshed with the double-sided rack, a first spring is fixedly arranged on one side of the double-sided rack, one end of the first spring is fixedly connected with the inner wall of the accommodating cavity, a clamping mechanism is arranged on the bearing plate and comprises a first belt wheel, the first belt wheel is fixedly connected with the second gear, two second belt wheels are rotatably arranged in the accommodating cavity, the first belt wheel and the two second belt wheels are provided with the same first belt in a transmission manner, the two second belt wheels are fixedly provided with screw rods, the two screw rods are in threaded connection with supporting rods, the two supporting rods are fixedly provided with the same circular ring, the circular ring is provided with a circular groove in a sliding manner, a pressing plate is arranged in the circular groove in a sliding manner, the circular groove is in threaded connection with a bolt, the bolt is in rotating connection with the pressing plate, an adjusting plate is arranged on the bolt, a centrifugal mechanism is arranged in the bottom box and comprises a centrifugal plate, the centrifugal plate is fixedly connected with a rotating rod, a plurality of fixing sleeves are arranged on the centrifugal plate, pressure sensors are arranged in the fixing sleeves, a plurality of pressure sensors are fixedly provided with second springs, a plurality of second springs are fixedly provided with U-shaped grooves, a plurality of U-shaped grooves are provided with test tubes, the rotating rod is fixedly provided with a third belt wheel, a fourth belt wheel is rotatably arranged in the accommodating cavity, and the same second belt is arranged on the third belt wheel and the fourth belt wheel in a transmission manner, fixed mounting has the swing arm on the fourth band pulley, slidable mounting has the rectangle pole on the swing arm, fixed mounting has the restriction box in the under casing, fixed mounting has first bearing on the restriction box, the inner circle of first bearing and the outside fixed connection of rectangle pole, be provided with vibrations mechanism in the under casing, vibrations mechanism includes first bevel gear, and first bevel gear and rectangle pole fixed connection have a second bevel gear in the meshing on the first bevel gear, and fixed mounting has the pole on the second bevel gear, and fixed mounting has the half-gear on the pole, and slidable mounting has the rack in the under casing, and the half-gear meshes with the rack mutually, and fixed mounting has the roof on the rack, roof and loading board fixed connection.
Preferably, the bearing plate is provided with a ring rail, the bottom of the centrifugal plate is provided with a plurality of rollers, the rollers are all in rolling connection with the ring rail, and the bottom box is provided with an observation window.
Preferably, the containing cavity is internally provided with a transverse rail, and the transverse rail is in sliding connection with the double-sided rack.
Preferably, the rotating rod is provided with a rectangular groove, and the inner walls of four sides of the rectangular groove are in sliding connection with four sides of the rectangular rod.
Preferably, a second bearing is fixedly installed in the accommodating cavity, and an inner ring of the second bearing is fixedly connected with one side of the first belt pulley.
Preferably, a vertical rail is arranged in the bottom box and is in sliding connection with the rack.
Preferably, the ring groove is provided with a groove rail, and the groove rail is connected with the ring in a sliding manner.
Compared with the prior art, the invention has the advantages that:
1. when the scheme is used, the centrifugal mechanism can rotate quickly, and the protein is separated by using the eccentricity, so that the protein is conveniently separated and extracted, and the extraction efficiency is improved.
2. This scheme is when using, and the test tube can be held to fixture's setting, avoids centrifugal force to throw away the test tube, causes the test tube to damage, causes the wasting of resources, and the setting of clamp plate, bolt and regulating plate can finely tune, and the size of change pressure that can be more careful avoids the test tube to damage.
3. This scheme is when using, and setting up of vibrations mechanism can drive the loading board and slide from top to bottom for vibrations when the centrifuging plate pivoted improve protein separation efficiency, improve the extraction efficiency of protein, improve the practicality of device.
The invention can improve the extraction efficiency, avoid the test tube from being thrown out, avoid unnecessary loss, and has simple use and convenient operation.
Drawings
FIG. 1 is a schematic perspective view of an extraction apparatus for cell-free protein synthesis according to the present invention;
FIG. 2 is a schematic cross-sectional view of an embodiment of an extraction apparatus for cell-free protein synthesis according to the present invention;
FIG. 3 is an enlarged schematic view of part A of FIG. 2 of an extraction apparatus for cell-free protein synthesis according to the present invention;
FIG. 4 is an enlarged schematic view of a portion B of the extraction apparatus for cell-free protein synthesis according to the present invention shown in FIG. 2;
FIG. 5 is a schematic view showing a connection structure of a half gear, a rack and a top plate in an embodiment of an extraction apparatus for cell-free protein synthesis according to the present invention;
FIG. 6 is a schematic view of the connection structure of the first gear, the second gear, the double-sided rack and the first spring of the extraction device for cell-free protein synthesis according to the present invention;
FIG. 7 is a schematic view showing a connection structure of a disk, a circular shaft, a middle plate and a top plate in a second embodiment of the extraction apparatus for cell-free protein synthesis according to the present invention.
In the figure: 1 bottom box, 2 bearing plate, 3 motor, 4 rotating rod, 5 first gear, 6 double-sided rack, 7 second gear, 8 first spring, 9 first belt wheel, 10 second belt wheel, 11 first belt, 12 screw, 13 support rod, 14 circular ring, 15 circular groove, 16 press plate, 17 bolt, 18 adjusting plate, 19 centrifugal plate, 20 fixed sleeve, 21 pressure sensor, 22 second spring, 23U-shaped groove, 24 test tube, 25 third belt wheel, 26 fourth belt wheel, 27 second belt, 28 rotating rod, 29 rectangular rod, 30 first bevel gear, 31 second bevel gear, 32 moving rod, 33 half gear, 34 toothed rack, 35 top plate, 36 limiting box, 37 first bearing, 38 containing cavity, 39 idler wheel, 40 observation window, 41 circular disk, 42 circular shaft, 43 Chinese character plate.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example one
Referring to fig. 1-6, an extraction device for cell-free protein synthesis comprises a bottom case 1, a bearing plate 2 is slidably mounted in the bottom case 1, a containing cavity 38 is formed on the bearing plate 2, a motor 3 is fixedly mounted in the containing cavity 38, a rotating rod 4 is fixedly mounted on an output shaft of the motor 3, a first gear 5 is fixedly mounted on the rotating rod 4, a double-sided rack 6 is engaged on the first gear 5, a second gear 7 is rotatably mounted in the containing cavity 38, the second gear 7 is engaged with the double-sided rack 6, a first spring 8 is fixedly mounted on one side of the double-sided rack 6, one end of the first spring 8 is fixedly connected with the inner wall of the containing cavity 38, a clamping mechanism is arranged on the bearing plate 2, a centrifugal mechanism is arranged in the bottom case 1, a third belt pulley 25 is fixedly mounted on the rotating rod 4, a fourth belt pulley 26 is rotatably mounted in the containing cavity 38, and a second belt 27 is mounted on the third belt pulley 25 and the fourth belt pulley 26 in a transmission manner, a rotating rod 28 is fixedly installed on the fourth belt wheel 26, a rectangular rod 29 is installed on the rotating rod 28 in a sliding mode, a limiting box 36 is fixedly installed in the bottom box 1, a first bearing 37 is fixedly installed on the limiting box 36, the inner ring of the first bearing 37 is fixedly connected with the outer side of the rectangular rod 29, and a vibration mechanism is arranged in the bottom box 1.
According to the invention, the clamping mechanism comprises a first belt wheel 9, the first belt wheel 9 is fixedly connected with a second gear 7, two second belt wheels 10 are rotatably mounted in an accommodating cavity 38, the same first belt 11 is mounted on the first belt wheel 9 and the two second belt wheels 10 in a transmission manner, screw rods 12 are fixedly mounted on the two second belt wheels 10, supporting rods 13 are connected to the two screw rods 12 in a threaded manner, the same circular ring 14 is fixedly mounted on the two supporting rods 13, a circular groove 15 is slidably mounted on the circular ring 14, a pressing plate 16 is slidably mounted in the circular groove 15, a bolt 17 is connected to the circular groove 15 in a threaded manner, the bolt 17 is rotatably connected with the pressing plate 16, and an adjusting plate 18 is arranged on the bolt 17.
In the invention, the centrifugal mechanism comprises a centrifugal plate 19, the centrifugal plate 19 is fixedly connected with a rotating rod 4, a plurality of fixing sleeves 20 are arranged on the centrifugal plate 19, pressure sensors 21 are arranged in the fixing sleeves 20, second springs 22 are fixedly arranged on the pressure sensors 21, U-shaped grooves 23 are fixedly arranged on the second springs 22, and test tubes 24 are arranged in the U-shaped grooves 23.
In the invention, the vibration mechanism comprises a first bevel gear 30, the first bevel gear 30 is fixedly connected with a rectangular rod 29, a second bevel gear 31 is meshed with the first bevel gear 30, a moving rod 32 is fixedly arranged on the second bevel gear 31, a half gear 33 is fixedly arranged on the moving rod 32, a rack 34 is slidably arranged in the bottom box 1, the half gear 33 is meshed with the rack 34, a top plate 35 is fixedly arranged on the rack 34, and the top plate 35 is fixedly connected with the bearing plate 2.
In the invention, the rotating rod 28 is provided with a rectangular groove, the inner walls of the four sides of the rectangular groove are in sliding connection with the four sides of the rectangular rod 29, and the rectangular groove can enable the rectangular rod 29 to rotate along with the rotating rod 28 without affecting sliding.
In the invention, the bearing plate 2 is provided with a circular rail, the bottom of the centrifugal plate 19 is provided with a plurality of rollers 39, the plurality of rollers 39 are all in rolling connection with the circular rail, and the bottom box 1 is provided with an observation window 40.
In the invention, a vertical rail is arranged in the bottom box 1, the vertical rail is connected with the tooth rack 34 in a sliding manner, and the vertical rail can enable the tooth rack 34 to slide at a fixed position.
In the invention, the ring groove 15 is provided with a groove rail, the groove rail is connected with the ring 14 in a sliding manner, and the groove rail can enable the ring groove 15 to slide on the ring 14.
In the invention, a transverse rail is arranged in the accommodating cavity 38, the transverse rail is connected with the double-sided rack 6 in a sliding manner, and the transverse rail can enable the double-sided rack 6 to slide at a fixed position.
In the invention, a second bearing is fixedly arranged in the accommodating cavity 38, the inner ring of the second bearing is fixedly connected with one side of the first belt pulley 9, and the second bearing can enable the first belt pulley 9 to rotate at a fixed position.
The working principle is as follows: when the test tube is used, the motor 3 is turned on, the rotating rod 4 is driven to rotate, the first gear 5 is driven to rotate, the double-sided rack 6 is driven to slide, the second gear 7 is driven to rotate, the first belt wheel 9 is driven to rotate, the two second belt wheels 10 are driven to rotate, the two screw rods 12 are driven to rotate, the two support rods 13 are driven to descend, the ring 14 is driven to descend, the ring 14 descends, the ring groove 15 and the pressing plate 16 are driven to descend and press on the test tube 24, the test tube 24 is prevented from being thrown out, when the double-sided rack 6 is separated from the second gear 7, the ring groove 15 is not moved, the adjusting plate 18 is rotated, the bolt 17 is driven to rotate, further driving the pressing plate 16 to slide for fine adjustment, the rotating rod 4 rotates, further driving the centrifugal plate 19 to rotate, protein is extracted by eccentricity, the rotating rod 4 rotates, further driving the third belt wheel 25 to rotate, the third belt wheel 25 rotates, further driving the fourth belt wheel 26 to rotate, the fourth belt wheel 26 rotates, further driving the rotating rod 28 to rotate, further driving the rectangular rod 29 to rotate, the rectangular rod 29 rotates, further driving the first bevel gear 30 to rotate, the first bevel gear 30 rotates, further driving the second bevel gear 31 to rotate, the bearing plate 31 rotates, further driving the moving rod 32 to rotate, the moving rod 32 rotates, further driving the half gear 33 to rotate, the half gear 33 rotates, further driving the rack 34 to slide up and down, further driving the top plate 35 to slide up and down, further driving the rack 2 to slide up and down, the bearing plate 2 slides up and down to drive the centrifugal plate 19 to vibrate, thereby improving the extraction efficiency.
Example two
Referring to fig. 7, the difference from the first embodiment is: the vibrating mechanism comprises a first bevel gear 30, the first bevel gear 30 is fixedly connected with a rectangular rod 29, a second bevel gear 31 is meshed on the first bevel gear 30, a moving rod 32 is fixedly installed on the second bevel gear 31, a disc 41 is fixedly installed on the moving rod 32, a circular shaft 42 is fixedly installed on the disc 41, a Chinese character plate 43 is slidably installed in the bottom box 1, the circular shaft 42 is slidably connected with the inner wall of the Chinese character plate 43, a top plate 35 is fixedly installed on the Chinese character plate 43, and the top plate 35 is fixedly connected with the bearing plate 2.
In the invention, the vertical rail is arranged in the bottom box 1 and is connected with the middle character plate 43 in a sliding way, and the vertical rail can enable the middle character plate 43 to slide at a fixed position.
The working principle is as follows: when the test tube is used, the motor 3 is turned on, the rotating rod 4 is driven to rotate, the first gear 5 is driven to rotate, the double-sided rack 6 is driven to slide, the second gear 7 is driven to rotate, the first belt wheel 9 is driven to rotate, the two second belt wheels 10 are driven to rotate, the two screw rods 12 are driven to rotate, the two support rods 13 are driven to descend, the ring 14 is driven to descend, the ring 14 descends, the ring groove 15 and the pressing plate 16 are driven to descend and press on the test tube 24, the test tube 24 is prevented from being thrown out, when the double-sided rack 6 is separated from the second gear 7, the ring groove 15 is not moved, the adjusting plate 18 is rotated, the bolt 17 is driven to rotate, further driving the pressing plate 16 to slide for fine adjustment, the rotating rod 4 rotates, further driving the centrifugal plate 19 to rotate, protein is extracted by eccentricity, the rotating rod 4 rotates, further driving the third belt wheel 25 to rotate, the third belt wheel 25 rotates, further driving the fourth belt wheel 26 to rotate, the fourth belt wheel 26 rotates, further driving the rotating rod 28 to rotate, further driving the rectangular rod 29 to rotate, the rectangular rod 29 rotates, further driving the first bevel gear 30 to rotate, the first bevel gear 30 rotates, further driving the second bevel gear 31 to rotate, further driving the moving rod 32 to rotate, the moving rod 32 rotates, further driving the disc 41 to rotate, the disc 41 rotates, further driving the circular shaft 42 to rotate, the circular shaft 42 rotates, further driving the middle plate 43 to slide up and down, further driving the top plate 35 to slide up and down, the top plate 35 slides up and down to further drive the loading plate 2 to slide up and down, and the loading plate 2 slides up and down to further drive the centrifugal plate 19 to vibrate, so that the extraction efficiency is improved.
The rest is the same as the first embodiment.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.