CN114081187A - Preservative-free biological activity application technology - Google Patents

Preservative-free biological activity application technology Download PDF

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Publication number
CN114081187A
CN114081187A CN202111228580.6A CN202111228580A CN114081187A CN 114081187 A CN114081187 A CN 114081187A CN 202111228580 A CN202111228580 A CN 202111228580A CN 114081187 A CN114081187 A CN 114081187A
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preservative
parts
biological activity
bacteria
extract
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陈志明
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Guangzhou Poya Biotechnology Co ltd
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Guangzhou Poya Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3526—Organic compounds containing nitrogen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/175—Amino acids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/123—Bulgaricus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/137—Delbrueckii
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention relates to the technical field of biological activity application, and discloses a preservative-free biological activity application technology, which comprises mixed bottom materials, distilled water, EM (effective microorganisms), Rw (microbial inoculum), plant essential oil and extract, protamine, tea polyphenol and propolis. A biological activity application technique without preservative comprises the following specific operation steps of taking the raw materials in corresponding parts for standby; mixing the mixed bottom material with distilled water, and adding the mixture into a mixing tank to obtain a mixed solution; sequentially adding plant essential oil and extract, protamine, tea polyphenols, and propolis into the above mixed solution; the biological reactant with higher activity is obtained by adding EM bacteria and RW bacteria agent into a mixing tank for full reaction, the biological activity application technology without the preservative is to respectively place four groups of samples into storage tanks for sealed storage and sampling experiments under the aseptic state, and the corruption degree of biological active substances in the four samples is detected, so that the optimal proportion can be obtained, and the biological activity can be kept, and the biological activity can be better preserved.

Description

Preservative-free biological activity application technology
Technical Field
The invention relates to the technical field of biological activity application, in particular to a preservative-free biological activity application technology.
Background
Bioactive substances, also called as physiologically active substances, i.e. compounds with biological activity, refer to trace or small amount of substances having influence on life phenomena, including polysaccharides, terpenes, sterols, alkaloids, peptides, nucleic acids, proteins, amino acids, glycosides, lipids, waxes, resins, phytochrome, mineral elements, enzymes, vitamins, etc., natural bioactive substances have physiological activities of anti-inflammation, anti-cancer, anti-oxidation, etc., are widely distributed in various animals and plants, marine organisms and microorganisms, fruits and vegetables are important components of daily diet, the currently identified main functional factors include dietary fibers, fructo-oligosaccharides, phytosterols, carotenoids, flavonoids, diallyl disulfide compounds, resveratrol, isothiocyanates, limonene, etc., such as apple is the main source of flavanol and flavonol in daily diet, has immunity enhancing and antioxidant effects; the longan polysaccharide has the health-care functions of regulating and improving immunity, memory, oxidation resistance, tumor resistance and the like; resveratrol contained in grape fruit and seed has effect in inhibiting lipid oxidation; the flavonoids in the nuts have the effects of inhibiting platelet aggregation, inhibiting bacteria, resisting tumors and the like, and the main bioactive substances in the vegetables comprise carotene, organic sulfur compounds, polysaccharides, lycopene, anthocyanin and the like in the flavonoids, so that the health of a human body can be remarkably promoted, diseases can be remarkably prevented, and the pumpkin polysaccharides have various health-care functions of food therapy, disease prevention and the like; the active chemical components in the balsam pear, such as triterpenes, steroids, alkaloids, proteins, organic acids, saccharides and the like, have the functions of reducing blood sugar and blood fat, regulating enzyme activity, resisting viruses, resisting tumors and the like.
The preservation conditions of the bioactive substances are harsh and are often easy to rot, so the preservation mode at present generally adopts a mode of adding the preservative to preserve, but the preservative is a chemical substance which is easy to damage the self structure of the bioactive substances, so that the efficacy of the bioactive substances is greatly reduced, and therefore, a preservative-free bioactive application technology is provided.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a preservative-free biological activity application technology, which has the advantages of preserving biological active substances without adding preservatives and improving the activity of the biological active substances, and solves the problem that the preservative is a chemical substance which is easy to damage the structure of the biological active substances and greatly reduces the efficacy of the active substances.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: a preservative-free bioactive application technology comprises the following materials in parts by weight:
mixing 140 parts of backing material 120;
80 parts of distilled water;
20-28 parts of EM (effective microorganisms)
24-30 parts of Rw microbial inoculum
40-50 parts of plant essential oil and extract
10-12 parts of protamine;
12-14 parts of tea polyphenol;
8-10 parts of propolis.
Preferably, the mixed bottom material comprises polypeptide and amino acid, and the amino acid is prepared into bioactive protein freeze-dried powder for use.
Preferably, the EM bacteria can secrete and synthesize organic acids, various enzymes and physiologically active substances.
Preferably, the Rw microbial inoculum is prepared by carrying out compound fermentation and purification on strains such as bifidobacterium, lactobacillus bulgaricus, lactobacillus delbrueckii, photosynthetic bacteria and the like, the total beneficial bacteria content per gram is more than or equal to 30 hundred million/CFU, and the earthworm extract can improve the beneficial bacteria flora in the environment and control pathogenic bacteria and putrefactive microorganism flora.
Preferably, the protamine is a fine and simple strong alkaline protein containing homoarginine, and has strong inhibiting effect on bacillus subtilis, bacillus megaterium, bacillus licheniformis, bacillus coagulans, lactobacillus plantarum, lactobacillus casei, streptococcus faecalis and the like.
Preferably, the plant essential oils and extracts comprise allicin, ginger, and honeysuckle leaves.
Preferably, according to the preservative-free bioactive application technology, the preservative-free bioactive application technology is provided with the following specific operation steps:
the method comprises the following steps: taking the raw materials in corresponding parts for later use;
step two: mixing the mixed bottom material with distilled water, and adding the mixture into a mixing tank to obtain a mixed solution;
step three: sequentially adding plant essential oil and extract, protamine, tea polyphenols, and propolis into the above mixed solution;
step four: and adding EM bacteria and RW bacterial agent into the mixing tank for full reaction to obtain the biological reactant with high activity.
Compared with the prior art, the invention provides a preservative-free biological activity application technology, which has the following beneficial effects:
1. the preservative-free biological activity application technology comprises the steps of conducting a preservation experiment on biological active substances prepared by matching three examples and one comparative example, wherein four samples are selected and respectively named as example one, example two, example three and comparative example one, the four groups of samples are respectively placed into a storage tank in a sterile state for sealed preservation in the experiment, sampling experiments are conducted respectively when the samples are preserved for one month, two months and three months, the corruption degree of the biological active substances in the four samples is detected, the corruption degree of the sample two is obviously slower than that of the other three groups of samples in the four groups of samples according to experimental data, the corruption degree of the sample four is fastest, the preservative effect is worst, the biological active substance in the sample two corresponding to each component in the example two is distributed with the best preservative effect, and therefore the best matching ratio can have higher preservative effect under the condition of keeping biological activity, and no medicament preservative is added in the preservative process, so that the preservative has a certain environment-friendly effect.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
a biological activity application technique without antiseptic comprises mixing base material, distilled water, EM bacteria, Rw bacteria agent, plant essential oil and extract, protamine, tea polyphenols, and propolis.
Specifically, the mixed bottom material comprises polypeptide and amino acid, and the amino acid is prepared into bioactive protein freeze-dried powder for use.
Specifically, the EM can secrete and synthesize organic acids, various enzymes and physiologically active substances.
Specifically, the Rw microbial inoculum is prepared by carrying out compound fermentation and purification on strains such as bifidobacterium, lactobacillus bulgaricus, lactobacillus delbrueckii, photosynthetic bacteria and the like, the total beneficial bacteria content per gram is more than or equal to 30 hundred million/CFU, and the earthworm extract can improve the beneficial bacteria flora in the environment and control pathogenic bacteria and putrefactive microorganism flora.
Specifically, the protamine is a fine and simple strong alkaline protein containing homoarginine, and has strong inhibiting effect on bacillus subtilis, bacillus megaterium, bacillus licheniformis, bacillus coagulans, lactobacillus plantarum, lactobacillus casei, streptococcus faecalis and the like.
Specifically, the plant essential oil and extract comprise allicin, ginger and honeysuckle leaves.
Specifically, according to the above-mentioned preservative-free bioactive application technique, a preservative-free bioactive application technique is proposed, which comprises the following specific operation steps:
the method comprises the following steps: preparing the following raw materials in percentage by mass:
120 parts of mixed bottom materials, 80 parts of distilled water, 20 parts of EM (effective microorganisms), 24 parts of Rw microbial inoculum, 40 parts of plant essential oil and extract, 10 parts of protamine, 12 parts of tea polyphenol and 8 parts of propolis;
step two: taking the raw materials in corresponding parts for later use;
step three: mixing the mixed bottom material with distilled water, and adding the mixture into a mixing tank to obtain a mixed solution;
step four: sequentially adding plant essential oil and extract, protamine, tea polyphenols, and propolis into the above mixed solution;
step five: and adding EM bacteria and RW bacterial agent into the mixing tank for full reaction to obtain the biological reactant with high activity.
Example two:
according to the preservative-free biological activity application technology, the preservative-free biological activity application technology is provided with the following specific operation steps:
the method comprises the following steps: preparing the following raw materials in percentage by mass:
130 parts of mixed bottom materials, 80 parts of distilled water, 24 parts of EM (effective microorganisms), 26 parts of Rw microbial inoculum, 45 parts of plant essential oil and extract, 11 parts of protamine, 13 parts of tea polyphenol and 9 parts of propolis;
step two: taking the raw materials in corresponding parts for later use;
step three: mixing the mixed bottom material with distilled water, and adding the mixture into a mixing tank to obtain a mixed solution;
step four: sequentially adding plant essential oil and extract, protamine, tea polyphenols, and propolis into the above mixed solution;
step five: and adding EM bacteria and RW bacterial agent into the mixing tank for full reaction to obtain the biological reactant with high activity.
Example three:
according to the preservative-free biological activity application technology, the preservative-free biological activity application technology is provided with the following specific operation steps:
the method comprises the following steps: preparing the following raw materials in percentage by mass:
140 parts of mixed bottom materials, 80 parts of distilled water, 28 parts of EM (effective microorganisms), 30 parts of Rw microbial inoculum, 50 parts of plant essential oil and extract, 12 parts of protamine, 14 parts of tea polyphenol and 10 parts of propolis;
step two: taking the raw materials in corresponding parts for later use;
step three: mixing the mixed bottom material with distilled water, and adding the mixture into a mixing tank to obtain a mixed solution;
step four: sequentially adding plant essential oil and extract, protamine, tea polyphenols, and propolis into the above mixed solution;
step five: and adding EM bacteria and RW bacterial agent into the mixing tank for full reaction to obtain the biological reactant with high activity.
Comparative example one:
according to the preservative-free biological activity application technology, the preservative-free biological activity application technology is provided with the following specific operation steps:
the method comprises the following steps: preparing the following raw materials in percentage by mass:
110 parts of mixed bottom materials, 80 parts of distilled water, 16 parts of EM (effective microorganisms), 20 parts of Rw microbial inoculum, 35 parts of plant essential oil and extract, 8 parts of protamine, 11 parts of tea polyphenol and 6 parts of propolis;
step two: taking the raw materials in corresponding parts for later use;
step three: mixing the mixed bottom material with distilled water, and adding the mixture into a mixing tank to obtain a mixed solution;
step four: sequentially adding plant essential oil and extract, protamine, tea polyphenols, and propolis into the above mixed solution;
step five: and adding EM bacteria and RW bacterial agent into the mixing tank for full reaction to obtain the biological reactant with high activity.
Experimental analysis:
the biological active substances prepared by the three examples and the comparative example are mixed to carry out a storage experiment, wherein four samples are selected and are respectively the first example, the second example, the third example and the first comparative example. In the experiment, four groups of samples are respectively placed in a storage tank for sealed storage in an aseptic state, sampling experiments are respectively carried out when the samples are stored for one month, two months and three months, the corruption degree of bioactive substances in the four samples is detected, and the experimental data are as follows
One month spoilage level (%):
a first sample: 20
Sample two: 16
Sample three: 23
Sample four: 25
Two month spoilage (%):
a first sample: 26
Sample two: 29
Sample three: 30
Sample four: 34
Three month spoilage (%):
a first sample: 35
Sample two: 34
Sample three: 40
Sample four: 60
According to the experimental data, the corruption degree of the second sample is obviously slower than that of the other three samples in the four groups of samples, the corruption degree of the fourth sample is the fastest, the corrosion prevention effect is the worst, and the corrosion prevention effect of the bioactive substance is the best under the distribution ratio of the components in the second embodiment corresponding to the second sample.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. A preservative-free bioactive application technology is characterized by comprising the following materials in parts by weight:
mixing 140 parts of backing material 120;
80 parts of distilled water;
20-28 parts of EM (effective microorganisms)
24-30 parts of Rw microbial inoculum
40-50 parts of plant essential oil and extract
10-12 parts of protamine;
12-14 parts of tea polyphenol;
8-10 parts of propolis.
2. The preservative-free bioactive application technique of claim 1, wherein: the mixed base material comprises polypeptide and amino acid, and the amino acid is prepared into bioactive protein freeze-dried powder for use.
3. The preservative-free bioactive application technique of claim 1, wherein: the EM bacteria can secrete and synthesize organic acids, various enzymes and physiologically active substances.
4. The preservative-free bioactive application technique of claim 1, wherein: the Rw microbial inoculum is prepared by carrying out compound fermentation and purification on bifidus bacteria, lactobacillus bulgaricus, lactobacillus delbrueckii, photosynthetic bacteria and other strains, wherein the total beneficial bacteria content per gram is more than or equal to 30 hundred million/CFU, and the earthworm extract can improve beneficial bacteria groups in the environment and control pathogenic bacteria and putrefactive microorganism groups.
5. The preservative-free bioactive application technique of claim 1, wherein: the protamine is a fine and simple strong alkaline protein containing homoarginine, and has strong inhibiting effect on bacillus subtilis, bacillus megaterium, bacillus licheniformis, bacillus coagulans, lactobacillus plantarum, lactobacillus casei, streptococcus faecalis and the like.
6. The preservative-free bioactive application technique of claim 1, wherein: the plant essential oil and extract comprises allicin, rhizoma Zingiberis recens, and folium Lonicerae.
7. The preservative-free bioactive application technology according to claim 1, which is characterized in that the specific operation steps of the preservative-free bioactive application technology are provided as follows:
the method comprises the following steps: taking the raw materials in corresponding parts for later use;
step two: mixing the mixed bottom material with distilled water, and adding the mixture into a mixing tank to obtain a mixed solution;
step three: sequentially adding plant essential oil and extract, protamine, tea polyphenols, and propolis into the above mixed solution;
step four: and adding EM bacteria and RW bacterial agent into the mixing tank for full reaction to obtain the biological reactant with high activity.
CN202111228580.6A 2021-10-21 2021-10-21 Preservative-free biological activity application technology Pending CN114081187A (en)

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Cited By (1)

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CN116515719A (en) * 2023-07-03 2023-08-01 中建易通科技股份有限公司 Microbial composite microbial agent for water quality improvement, preparation method and application thereof

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