CN114010844B - Membrane material with stable signal transmission function, myocardial patch and preparation method thereof - Google Patents
Membrane material with stable signal transmission function, myocardial patch and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
- A61L27/446—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with other specific inorganic fillers other than those covered by A61L27/443 or A61L27/46
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
- A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/22—Lipids, fatty acids, e.g. prostaglandins, oils, fats, waxes
- A61L2300/222—Steroids, e.g. corticosteroids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/41—Anti-inflammatory agents, e.g. NSAIDs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/20—Materials or treatment for tissue regeneration for reconstruction of the heart, e.g. heart valves
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a membrane material with stable signal transmission function, a myocardial patch and a preparation method thereof, in particular to a membrane material with stable signal transmission function, which is prepared from Polyurethane (PU) and silver nitrate (AgNO) 3 ) Graphene Oxide (GO) and Methylprednisolone (MP) are used as spinning raw materials, N-Dimethylformamide (DMF) and Tetrahydrofuran (THF) are used as solvents to prepare spinning solution, and a nanofiber membrane is prepared by an electrostatic spinning method. The prepared film material has good conductivity, stable fluctuation of electric signals, high elasticity and high fracture strain. In addition, under the wet condition, the performance can be kept stable, the material can effectively play a role as a myocardial patch, and the invention not only can provide guidance and optimization for the design and manufacture of the myocardial patch, but also has important reference value for clinical application. The novel myocardial patch material with various performances prepared by the invention makes an important contribution to the development of textiles in the medical direction.
Description
Technical Field
The invention belongs to the technical field of tissue engineering, relates to a myocardial tissue engineering material, and in particular relates to a membrane material with a stable signal transmission function, a myocardial patch and a preparation method thereof.
Background
Myocardial infarction (myocardial infarction, MI) is the leading disabling and fatal disease worldwide, the result of coronary artery occlusion, one of the leading causes of cardiovascular disease today. Myocardial infarction seriously jeopardizes the physical health and life safety of people, and the lack of self-repair capability of myocardial tissue is one of the challenges in the research field of treating cardiovascular diseases. The current methods for treating myocardial infarction include drug therapy, interventional therapy, thrombolytic therapy, coronary artery bypass surgery, traditional Chinese medicine therapy and the like. These traditional treatments are difficult to repair damaged myocardial tissue and fail to restore necrotic and fibrotic myocardium to normal, so it is imperative to improve the efficacy of traditional treatments and find new treatment strategies.
Tissue engineering is an emerging discipline of combining cellular biology and materials to construct tissues or organs in vitro or in vivo. The advent and rapid development of myocardial tissue engineering (Cardiac tissue engineering) has made it a growing focus of research in repair of MI with the goal of constructing tissue engineered myocardial tissue to repair or replace damaged myocardium. Myocardial tissue engineering is an important potential approach to repair damaged myocardial tissue, and tissue engineering materials can be used for the delivery of stem cells (bone marrow mesenchymal stem cells, embryonic stem cells, etc.), growth factors (VEGF, IL-7, etc.), and mimic extracellular matrix. The main research in this field, including the delivery of scaffold materials, stem cells, and growth factors, to repair myocardium, has now made significant progress.
Since cardiomyocytes are sensitive to ischemia and contractile, the conductivity, elasticity, stretchability, mechanical stability, wet stability, and the like of the material are considered. The myocardial tissue engineering material is a material which can provide proper environment for cell or tissue growth, has special functions and is used for organism tissue repair and regeneration, and is mainly divided into natural materials and artificial synthetic materials. Natural materials include collagen, chitosan, sodium alginate, hyaluronic acid, and the like. The synthetic material mainly comprises polyester, polylactic acid (PLA), polylactic acid-glycolic acid copolymer (PLGA) and the like, and has the main advantages of stable chemical characteristics, strong controllability, accurate mechanical and shape design and the like, and the defects of possibly causing organism inflammatory reaction, slow degradation and the like of degradation products. With the rapid development of technology, the material gradually realizes the span from millimeter level, micron level to nanometer level (i.e. nanometer material), thereby achieving the purpose of optimizing the material. The myocardial patch is electrically conductive, highly elastic, and requires good biocompatibility and biodegradability to provide proper mechanical support to the heart chamber. The myocardial patch not only has excellent performance, but also needs to have certain mechanical strength in consideration of various factors of the myocardial patch acting in vivo and in vitro, and can provide support for ventricles, prevent the ventricles from further expanding, and the problems at present can be rejection of foreign tissues. Secondly, the main problems of the current myocardial patch for treating myocardial infarction are poor electrical conductivity and easy interruption of electromechanical signals. To increase the conductivity of the myocardial patch, certain metal particles, graphene, carbon nanotubes, etc. may be added to the myocardial patch by electrospinning techniques. Another important problem is that elastic deformation is not ideal and does not provide sustained systolic function. In contrast, elastic biomaterials are more conductive and elastic patches can provide appropriate mechanical support for the heart. It has been reported that complete pathological remodeling after myocardial infarction takes about 6 weeks, so cardiac patches need to have good biocompatibility and biodegradability, should degrade relatively slowly and can provide long-term mechanical support. Myocardial patches have become a hope for myocardial repair and regeneration due to their numerous advantages in theory. However, for clinical applications, there are many practical difficulties with current materials to be overcome.
Disclosure of Invention
The invention aims at overcoming the defects in the prior art and provides a membrane material with stable signal transmission function, a myocardial patch and a preparation method thereof; the membrane material has good and stable electric signal conduction function in both a dry state and a wet state, has high elasticity, and can achieve excellent repairing effect especially when being applied as a myocardial patch.
The technical scheme adopted by the invention is as follows:
a membrane material with stable signal transmission function, comprising the following components: polyurethane (PU), silver nitrate (AgNO) 3 ) Graphene Oxide (GO), methylprednisolone (MP);the membrane material is a nanofiber membrane obtained by mixing the components according to a specific proportion as a solute, adding the solute into a solvent consisting of N, N-Dimethylformamide (DMF) and Tetrahydrofuran (THF) to obtain a spinning solution, and carrying out electrostatic spinning.
Further, PU and AgNO in the spinning solution 3 The mass ratio of GO to MP is 500:50:3:50.
Further, the mass ratio of DMF to THF is 1:1.
Furthermore, the membrane material can be used as a cardiac muscle patch to be applied to the field of cardiac tissue engineering, and particularly, when the diameter of the nanofiber in the membrane material is 0.35-1.1 mu m, and the thickness of the membrane material is 0.17-0.20mm, a good repairing effect can be achieved.
Further, the mass ratio of the solvent to the solute in the spinning solution is 15.6:1.
Further, the spinning solution is obtained by the following method: agNO was added to a mixture of DMF and THF 3 After GO and MP are completely dispersed by ultrasound, PU is added, and the PU is completely dissolved by heating and stirring to obtain uniform and stable spinning solution. Further, the heating and stirring temperature is 60 ℃ and stirring is carried out for at least 3 hours, and the ultrasonic time is at least 60 minutes.
Further, the parameters of the electrostatic spinning are as follows: the temperature is 40.0 ℃, the humidity is 23.1%, the spinning distance is 11cm, the liquid supply speed is 2mL/h, the sliding table speed is 15mm/s, the rotating speed of the roller is 300rad/min, and the voltage is 15kV.
AgNO is added into the invention 3 And then, the prepared nanofiber membrane has good conductivity, and the electric signal stably fluctuates. Further addition of GO further increases conductivity. The PU material can strengthen the elasticity and mechanical property of the cardiac muscle patch. The prepared myocardial patch was found to be electrically conductive and stable. After stretching 10% and 20%, the electrical signal fluctuations increase but are still relatively uniform and stable. Pure PU and AgNO addition 3 The contact angle with the nanofiber membrane of GO was greater than 90 °, the nanofiber membrane was all hydrophobic, while the contact angle with the nanofiber membrane after further addition of MP was less than 90 °, it was found that the membrane was changed from hydrophobic to hydrophilic. Prepared heartThe muscle patch can be kept stable after being soaked in PBS solution and DMEM culture medium, and can not fall off, so that the adhesion and stability of the muscle patch are good. To further conclude, the electrical signal obtained after the inventive myocardial patch was immersed in PBS solution for 1, 3, 5, 7 days was still good, and it was found that no failure occurred in the wet environment. The myocardial patch is attached to the cattle heart, so that the myocardial patch can be firmly attached to the cattle heart, the shedding and the cracking can not occur, the air is injected and sucked out, and the myocardial patch can be matched with the diastole and systole activities during the heart beating. And then the myocardial patch is attached to the finger, the elbow fossa and the back of the hand, so that the induction degree of different parts to the electric signals can be found to be different. The fingerprint unlocking device can simulate human skin to operate the smart phone and can also be well used for unlocking the fingerprint. It can be seen that the application of the myocardial patch material of the present invention is relatively wide.
The invention has the beneficial effects that:
the method has simple process, and the invention explores a novel myocardial patch which integrates the performances of conductivity, high elasticity, adhesiveness, wet stability and the like. The prepared myocardial patch has good conductivity, can organically integrate electric signals between infarcted parts and healthy myocardial tissues, improves myocardial electric conduction and adjusts arrhythmia. The myocardial patch has high elasticity and is matched with the diastole and the systole of the heart to relax and contract. In a wet environment, the electrical signals of the myocardial patch are still good, and no failure occurs. The method is a new research on a treatment method for treating the cardiac function impairment, and plays an important role in inhibiting myocardial infarction area, reconstructing damaged myocardial cells, recovering cardiac function and the like. The invention research on the myocardial patch can provide guidance and optimization for the design and manufacture of the myocardial patch, and has important reference value for clinical application. The novel myocardial patch with various performances prepared by the invention contributes to the development of textiles in the medical direction.
Drawings
FIG. 1 is a scanning electron micrograph (3500 times) of a nanofiber membrane prepared in example 1;
FIG. 2 is a scanning electron micrograph (3500 times) of the nanofiber membrane prepared in example 2;
FIG. 3 is a photograph of the electrical signal (tiling) of the nanofiber membrane prepared in example 2;
FIG. 4 is a scanning electron micrograph (3500 times) of the nanofiber membrane prepared in example 3;
FIG. 5 is an electrical signal photograph (tiling) of the nanofiber membrane prepared in example 3;
FIG. 6 is a scanning electron micrograph (3500 times) of the nanofiber membrane prepared in example 4;
FIG. 7 is an electrical signal photograph (tiling) of the nanofiber membrane prepared in example 4;
FIG. 8 is a nanofiber membrane prepared in example 4 immersed in PBS solution;
FIG. 9 is a nanofiber membrane prepared in example 4 immersed in DMEM medium;
FIG. 10 is a schematic illustration of the nanofiber membrane prepared in example 4 attached to a cow's heart;
FIG. 11 is a photograph showing the dipping of the heart of a cow to which the myocardial patch prepared in example 4 was attached in PBS solution;
FIG. 12 is a graph showing the immersion of the heart of a cow to which the myocardial patch prepared in example 4 was attached in DMEM medium.
Detailed Description
The technical scheme of the invention is further described below with reference to specific embodiments and drawings.
Example 1:
a clean glass stirring bottle was taken, a magnet was placed therein, and 0.5g PU was weighed with an electronic balance and placed in DMF/THF (1/1) = 4.8395 g. Stirring for 3 hours at 60 ℃ in a digital display temperature-control heating stirrer to ensure that PU is completely dissolved, and finally obtaining uniform and stable spinning solution. The electrostatic spinning parameters are as follows: the temperature is 40.0 ℃, the humidity is 23.1%, the spinning distance is 11cm, the liquid supply speed is 2mL/h, the sliding table speed is 15mm/s, the rotating speed of the roller is 300rad/min, and the voltage is 15kV. And (3) drying the prepared nanofiber membrane in an oven at 60 ℃ for 10min. A nanofiber myocardial patch with elasticity is obtained, but with little conductivity.
The prepared myocardial patch had an average diameter of 0.35 μm, an average thickness of 0.12mm, and air permeability of 737.48mm/s, and was hydrophobic as the water drops remained on the nanofiber surface with a contact angle of 101.71 °. The tensile stress and strain of the myocardial patch were 365959Pa and 216%, respectively.
Example 2:
a clean glass stirring bottle was taken, a magnet was placed therein, and 0.5g of Polyurethane (PU) and 0.05g of silver nitrate (AgNO) were weighed with an electronic balance 3 ) Placed in DMF/THF (1/1) = 4.7250 g. Stirring for 3 hours at 60 ℃ in a digital display temperature-controlled heating stirrer to ensure that PU and AgNO 3 And completely dissolving to finally obtain uniform and stable spinning solution. The electrostatic spinning parameters are as follows: the temperature is 40.0 ℃, the humidity is 23.1%, the spinning distance is 11cm, the liquid supply speed is 2mL/h, the sliding table speed is 15mm/s, the rotating speed of the roller is 300rad/min, and the voltage is 15kV. And (3) drying the prepared nanofiber membrane in an oven at 60 ℃ for 10min.
The prepared myocardial patch had an average diameter of 1.1 μm, an average thickness of 0.11mm, and air permeability of 1145.58mm/s, and was hydrophobic as the water drops remained on the nanofiber surface and from a contact angle of 105.3 °. The tensile stress and strain of the myocardial patch were 542954Pa and 140%, respectively.
Example 3:
a clean glass stirring bottle was taken, a magnet was placed therein, and 0.05g of silver nitrate (AgNO) was weighed with an electronic balance 3 ) Placed in DMF/THF (1/1) = 4.7235 g. Then weighing 0.003g of Graphene Oxide (GO), putting into an ultrasonic cleaner, and performing ultrasonic treatment for 60min to ensure that the GO is completely dispersed in the solution. Then, 0.5g of PU was added thereto, and the total mass of the spinning solution was 10g. Stirring for 3 hours at 60 ℃ in a digital display temperature-control heating stirrer to ensure that PU is completely dissolved, and finally obtaining uniform and stable spinning solution. The electrostatic spinning parameters are as follows: the temperature is 40.0 ℃, the humidity is 23.1%, the spinning distance is 11cm, the liquid supply speed is 2mL/h, the sliding table speed is 15mm/s, the rotating speed of the roller is 300rad/min, and the voltage is 15kV.
The prepared myocardial patch had an average diameter of 0.83 μm, an average thickness of 0.14mm, and air permeability of 1424.75mm/s, and was hydrophobic as the water drops remained on the nanofiber surface with a contact angle of 98.3 °. The tensile stress and strain of the myocardial patch were 741539Pa and 124%, respectively.
Example 4:
a clean glass stirring bottle was taken, a magnet was placed therein, and 0.05g of silver nitrate (AgNO) was weighed with an electronic balance 3 ) 0.003g of Graphene Oxide (GO) and 0.05g of Methylprednisolone (MP) were placed in DMF/THF (1/1) = 4.6985g, placed in an ultrasonic cleaner, and sonicated for 60min, so that GO and MP were completely dispersed in the solution. Then, 0.5g of PU was added thereto, and the total mass of the spinning solution was 10g. Stirring for 3 hours at 60 ℃ in a digital display temperature-control heating stirrer to ensure that PU is completely dissolved, and finally obtaining uniform and stable spinning solution. The electrostatic spinning parameters are as follows: the temperature is 40.0 ℃, the humidity is 23.1%, the spinning distance is 11cm, the liquid supply speed is 2mL/h, the sliding table speed is 15mm/s, the rotating speed of the roller is 300rad/min, and the voltage is 15kV.
The prepared myocardial patch had an average diameter of 0.71 μm, an average thickness of 0.19mm, and air permeability of 1358.13mm/s, and was hydrophilic as a result of water drops entering inside the nanofibers, from a contact angle of 58.9 °.
The DM3068 digital multimeter is used for testing the electric signal, the voltage fluctuates up and down with 0.0076mV as a node when the nanofiber membrane is tiled, the voltage fluctuates up and down with 0.0029mV as a node when the nanofiber membrane is stretched by 10%, and the voltage fluctuates up and down with 0.0086mV as a node when the nanofiber membrane is stretched by 20%, so that the electric signal can be kept uniform and stable when the nanofiber membrane is stretched. The nanofiber membrane was subjected to tensile testing by means of an LES-G1 tensile tester, and the stress was 462139Pa and the strain was 149%. The myocardial patch was kept stable by soaking in both PBS solution and DMEM medium.
Adopts alpha-n-butyl cyanoacrylate (chemical formula is C 8 H 11 O 2 ) The medical adhesive of the patch is used for attaching the nanofiber membrane to the cattle heart for testing, so that the myocardial patch can be observed to be firmly attached to the cattle heart, air is injected and sucked out, falling and cracking cannot occur, and the myocardial patch can be matched with the diastole and systole activities during heart beating. Cattle to which myocardial patch is to be attachedThe hearts were immersed in PBS solution and DMEM medium, were still stable and did not fall off or break.
Claims (4)
1. A membrane material with stable signal transmission function, which is characterized by comprising the following components: polyurethane (PU), silver nitrate (AgNO) 3 ) Graphene Oxide (GO), methylprednisolone (MP); the membrane material is a nanofiber membrane obtained by mixing the components according to a specific proportion as a solute, adding the solute into a solvent consisting of N, N-Dimethylformamide (DMF) and Tetrahydrofuran (THF) to obtain a spinning solution, and carrying out electrostatic spinning on the spinning solution to obtain a nanofiber membrane, wherein PU and AgNO in the spinning solution of electrostatic spinning 3 The mass ratio of GO to MP is 500:50:3:50, the mass ratio of DMF to THF is 1:1, and the mass ratio of solvent to solute in the spinning solution is 15.6:1.
2. A myocardial patch in accordance with claim 1, wherein the diameter of the nanofibers in the membrane material is 0.35-1.1 μm, and the thickness of the membrane material is 0.17-0.20mm.
3. A myocardial patch as in claim 2 wherein the spin fluid is obtained by: agNO was added to a mixture of DMF and THF 3 After GO and MP are completely dispersed by ultrasound, PU is added, and the PU is completely dissolved by heating and stirring to obtain uniform and stable spinning solution, wherein the ultrasound time is at least 60min, and the heating and stirring temperature is 60 ℃ and stirring is at least 3h.
4. A myocardial patch as in claim 2 wherein the parameters of the electrospinning are as follows: the temperature is 40.0 ℃, the humidity is 23.1%, the spinning distance is 11cm, the liquid supply speed is 2mL/h, the sliding table speed is 15mm/s, the roller rotating speed is 300rad/min, and the voltage is 15kV.
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| CN115352164B (en) * | 2022-09-14 | 2023-07-25 | 陕西科技大学 | Preparation method of heart regeneration patch material based on piezoelectric nano generator |
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