CN107349473B - Degradable polylactic acid/fibroin/chitosan composite nerve conduit and preparation method thereof - Google Patents
Degradable polylactic acid/fibroin/chitosan composite nerve conduit and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/32—Materials or treatment for tissue regeneration for nerve reconstruction
Abstract
The invention belongs to the field of medical biomaterials, and particularly relates to a degradable polylactic acid/fibroin/chitosan composite nerve conduit and a preparation method thereof. The composite nerve conduit consists of polylactic acid, silk fibroin and chitosan, wherein the polylactic acid and the silk fibroin are prepared into an inner membrane of the nerve conduit through an electrostatic spinning method, the polylactic acid is prepared into an outer membrane of the nerve conduit on the inner membrane of the conduit through the electrostatic spinning method, the prepared nerve conduit is immersed into a chitosan solution through a solution immersion method, and the chitosan solution is put into a vacuum drying oven for solution volatilization to prepare the polylactic acid/silk fibroin/chitosan composite nerve conduit. The composite nerve conduit has good mechanical compression resistance, biocompatibility, cell affinity and appropriate degradation performance, and can effectively improve cell activity and inhibit formation of nerve scar and nerve tumor; has a bionic structure simulating extracellular matrix, and is beneficial to the adhesion, proliferation and migration of cells in the catheter.
Description
Technical Field
The invention belongs to the technical field of medical biomaterials, and particularly relates to a degradable polylactic acid/silk fibroin/chitosan composite nerve conduit and a preparation method thereof.
Background
Peripheral nerve injury is a common disease in daily life, short-distance nerve defects can be repaired by autografting, but if the nerve defects are too long, the autografting has the problem of insufficient donors, and donor area infection, scar formation and the like limit the clinical application of the autografting in peripheral nerve repair. Therefore, the research of the artificial nerve conduit is urgent, the material for the traditional artificial nerve conduit is not easy to degrade, the biocompatibility is not good, the preparation method is relatively backward, and the actual nerve repair requirement can not be well met.
In recent years, with the continuous research of artificial nerve conduits, great progress has been made, but still many problems exist, such as insufficient pressure resistance of the nerve conduit, easy extrusion by surrounding tissues, and catheter collapse; poor biocompatibility, inflammation, easy formation of nerve scar, tissue adhesion and other problems. In order to achieve the ideal repairing effect, the artificial nerve conduit should satisfy the following requirements: has good biocompatibility; has certain mechanical property and compressive strength; the material is non-toxic or slightly toxic; the degradation is easy, and the degradation product does not harm the organism; the controllability, the requirement of the adjustable conduit length, the degradation rate and the like for actual repair.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a degradable polylactic acid/fibroin/chitosan composite nerve conduit and a preparation method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
a preparation method of a degradable polylactic acid/silk fibroin/chitosan composite nerve conduit comprises the following steps:
(1) dissolving polylactic acid and silk fibroin in an organic solvent, electromagnetically stirring until the polylactic acid and the silk fibroin are fully dissolved to obtain a spinning solution 1, and spraying the spinning solution 1 on a rotating needle tube by an electrostatic spinning technology to prepare an inner membrane of a nerve conduit;
(2) dissolving polylactic acid in an organic solvent, electromagnetically stirring until the polylactic acid is fully dissolved to obtain a spinning solution 2, spraying the spinning solution 2 on the inner layer membrane of the nerve conduit obtained in the step (1) by using an electrostatic spinning technology, and preparing an outer layer membrane of the nerve conduit on the inner layer membrane of the nerve conduit;
(3) and (3) dissolving chitosan in a weak acid solution to obtain a chitosan solution with a certain concentration, immersing the nerve conduit consisting of the inner layer membrane and the outer layer membrane obtained in the step (2) into the chitosan solution for a period of time, taking out the nerve conduit, and putting the nerve conduit into a vacuum drying oven to remove the organic solvent, thus obtaining the polylactic acid/fibroin/chitosan composite nerve conduit.
According to the scheme, the inner aperture of the polylactic acid/fibroin/chitosan composite nerve conduit is 1.5-2 mm.
According to the scheme, the total thickness of the polylactic acid/fibroin/chitosan composite nerve conduit is 0.05-0.1 mm, and the thickness of an outer layer membrane is 0.05-0.1 mm. The shape and the length of the composite nerve conduit can be set according to actual needs.
According to the scheme, the mass ratio of the polylactic acid to the silk fibroin in the step (1) is 1: 3-1: 6.
According to the scheme, the parameters of electrostatic spinning in the step (1) are as follows: the flow rate of the spinning solution is 0.03-0.5 mm/min, the negative high voltage is-1 kV to-3 kV, the positive high voltage is +7kV to +17kV, the distance from the receiving distance to the needle head is 5-20 cm, and the rotating speed is 200-2000 r/min.
According to the scheme, the mass fraction of the polylactic acid in the spinning solution 2 in the step (2) is 8-20%.
According to the scheme, the parameters of the electrostatic spinning in the step (2) are as follows: the flow rate of the spinning solution is 0.03-0.5 mm/min, the negative high voltage is-0.5 kV to-3 kV, the positive high voltage is +7kV to +17kV, the distance from the receiving distance to the needle head is 8-18 cm, and the rotating speed is 200-2000 r/min.
According to the scheme, the mass concentration of chitosan in the chitosan solution in the step (3) is 5-15%, and the nerve conduit is immersed in the chitosan solution for 10-60 minutes.
According to the scheme, the molecular weight of the polylactic acid is 60000-320000 Da, and the molecular weight of the chitosan is 50000-300000 Da.
According to the scheme, the organic solvent in the step (1) and the step (2) is: mixing dichloromethane and ethyl acetate according to the volume ratio of 7:4 to obtain a mixture; and (4) the weak acidic solution in the step (3) is dilute acetic acid.
The polylactic acid/fibroin/chitosan composite nerve conduit prepared by the preparation method is provided.
The invention has the beneficial effects that:
(1) the polylactic acid/fibroin/chitosan composite nerve conduit has good mechanical property, biocompatibility, cell affinity and appropriate degradation performance, and can improve cell activity and inhibit formation of nerve scars and nerve tumors; the composite nerve conduit prepared by the electrostatic spinning method has a bionic structure, can simulate extracellular matrix, is beneficial to adhesion, proliferation and migration of cells in the conduit and promotes nerve regeneration; meanwhile, the aperture of the composite nerve conduit has a semi-permeable structure, which is beneficial to the entering of nutrient substances (nerve growth factors and glucose), prevents the entering of lymph and fibroblasts and better repairs damaged peripheral nerves;
(2) degradation products of all raw materials of the composite nerve conduit can be absorbed by a body, and the composite nerve conduit does not need to be taken out after a secondary operation, so that the pain of a patient is relieved;
(3) the composite nerve conduit has the advantages of simple preparation method, low cost, high preparation efficiency and huge potential application value.
Drawings
FIG. 1 is a Scanning Electron Microscope (SEM) photograph of the inner layer membrane of the composite nerve conduit prepared by the present invention.
Fig. 2 is a Scanning Electron Microscope (SEM) photograph of the inner layer membrane of the composite nerve conduit prepared by the present invention.
Fig. 3 is a Scanning Electron Microscope (SEM) photograph of the composite nerve conduit outer layer membrane prepared by the present invention.
Fig. 4 is a photograph of the inner pore diameter of the composite nerve conduit prepared by the present invention.
Fig. 5 is a photograph of the composite nerve conduit prepared by the present invention.
Detailed Description
In order to better understand the present invention, the following examples are further provided to illustrate the present invention, but the present invention is not limited to the following examples.
In the following examples, the silk fibroin can be prepared by the following method: boiling domestic silk in a certain amount of weakly alkaline aqueous solution to remove sericin, washing the sericin-removed solution in deionized water, oven drying at 60 deg.C, drying, and dissolving in ternary solution (CaCl)2Ethanol and water), then dialyzing with deionized water to obtain a silk fibroin solution, filtering, drying and putting into a refrigerator at 4 ℃ for later use. The domestic silk boiling time is 6h, the drying temperature is 60 ℃, the drying time is 12h, CaCl in the ternary solution2: ethanol: the water mass ratio is 1:2: 8.
In the following examples, the molecular weight of the polylactic acid is 60000-320000 Da, and the molecular weight of the chitosan is 50000-300000 Da.
Example 1
Weighing 0.2g of polylactic acid and 0.8g of prepared and dried silk fibroin by an electronic balance, dissolving the polylactic acid and the prepared and dried silk fibroin in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), stirring for 6h by using an electromagnetic stirrer, fully dissolving the polylactic acid and the silk fibroin to obtain uniform and stable polylactic acid/silk fibroin spinning solution 1, adopting a No. 20 needle head, controlling spinning parameters (spinning solution flow rate is 0.2mm/min, positive high voltage +11kV, negative high voltage-2 kV, distance between a receiving device and an injection needle head is 14cm, and rotating speed is 1200r/min), and spraying the spinning solution 1 onto a rotating round needle tube (inner diameter is 1.5mm) to prepare a nerve conduit inner membrane with the wall thickness of 0.15 mm; similarly, 1.3g of polylactic acid is weighed by an electronic balance to be dissolved in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), an electromagnetic stirrer is used for stirring for 6h to fully dissolve the polylactic acid to obtain uniform and stable polylactic acid spinning solution 2, a No. 21 needle is adopted, spinning parameters (the flow rate of the spinning solution is 0.2mm/min, positive high pressure +10kV, negative high pressure-2 kV, the distance between a receiving device and a jet needle is 15cm, and the rotating speed is 1300r/min) are controlled, the polylactic acid/fibroin spinning solution 2 is sprayed on the inner membrane of the nerve conduit to prepare the outer membrane of the nerve conduit with the thickness of 0.35 mm; soaking the nerve conduit composed of the inner layer membrane and the outer layer membrane into a chitosan solution with the concentration of 8%, taking out after soaking for 10min, putting into a vacuum drying oven to remove residual organic solvent and moisture to obtain the polylactic acid/fibroin/chitosan composite nerve conduit, and storing in the drying oven.
Example 2
Weighing 0.25g of polylactic acid and 1.0g of prepared and dried silk fibroin by an electronic balance, dissolving the polylactic acid and the prepared and dried silk fibroin in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), stirring for 6h by using an electromagnetic stirrer, fully dissolving the polylactic acid and the silk fibroin to obtain uniform and stable polylactic acid/silk fibroin spinning solution 1, adopting a No. 20 needle head, controlling spinning parameters (spinning solution flow rate is 0.25mm/min, positive high voltage +12kV, negative high voltage-3 kV, distance between a receiving device and an injection needle head is 14cm, and rotating speed is 1200r/min), and spraying the spinning solution 1 onto a rotating round needle tube (inner diameter is 1.5mm) to prepare a nerve conduit inner membrane with the wall thickness of 0.15 mm; similarly, 1.4g of polylactic acid is weighed by an electronic balance to be dissolved in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), an electromagnetic stirrer is used for stirring for 6h to fully dissolve the polylactic acid to obtain uniform and stable polylactic acid spinning solution 2, a No. 21 needle is adopted, spinning parameters (the flow rate of the spinning solution is 0.25mm/min, positive high pressure +10kV, negative high pressure-3 kV, the distance between a receiving device and a jet needle is 15cm, and the rotating speed is 1200r/min) are controlled, the polylactic acid/fibroin spinning solution 2 is sprayed on the inner membrane of the nerve conduit to prepare the outer membrane of the nerve conduit with the thickness of 0.35 mm; soaking the nerve conduit composed of the inner layer membrane and the outer layer membrane into a chitosan solution with the concentration of 8%, taking out after soaking for 10min, putting into a vacuum drying oven to remove residual organic solvent and moisture to obtain the polylactic acid/fibroin/chitosan composite nerve conduit, and storing in the drying oven.
Example 3
Weighing 0.3g of polylactic acid and 1.2g of prepared and dried silk fibroin by an electronic balance, dissolving the polylactic acid and the prepared and dried silk fibroin in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), stirring for 6h by using an electromagnetic stirrer, fully dissolving the polylactic acid and the fibroin to obtain uniform and stable polylactic acid/fibroin spinning solution 1, adopting a No. 20 needle head, controlling spinning parameters (spinning solution flow rate is 0.3mm/min, positive high voltage +13kV, negative high voltage-3 kV, distance between a receiving device and a jet needle head is 14cm, and rotating speed is 1200r/min), and spraying the spinning solution 1 onto a rotating round needle tube (inner diameter is 1.5mm) to prepare a nerve conduit inner membrane with the wall thickness of 0.15 mm; similarly, 1.5g of polylactic acid is weighed by an electronic balance to be dissolved in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), an electromagnetic stirrer is used for stirring for 6h to fully dissolve the polylactic acid to obtain uniform and stable polylactic acid spinning solution 2, a No. 21 needle is adopted, spinning parameters (the flow rate of the spinning solution is 0.3mm/min, positive high pressure +11kV, negative high pressure-3 kV, the distance between a receiving device and a jet needle is 15cm, and the rotating speed is 1200r/min) are controlled, the polylactic acid/fibroin spinning solution 2 is sprayed on the inner membrane of the nerve conduit to prepare the outer membrane of the nerve conduit with the thickness of 0.35 mm; soaking the nerve conduit composed of the inner layer membrane and the outer layer membrane into a chitosan solution with the concentration of 8%, taking out after soaking for 10min, putting into a vacuum drying oven to remove residual organic solvent and moisture to obtain the polylactic acid/fibroin/chitosan composite nerve conduit, and storing in the drying oven.
Example 4
Weighing 0.35g of polylactic acid and 1.4g of prepared and dried silk fibroin by an electronic balance, dissolving the polylactic acid and the prepared and dried silk fibroin in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), stirring for 6h by using an electromagnetic stirrer, fully dissolving the polylactic acid and the silk fibroin to obtain uniform and stable polylactic acid/silk fibroin spinning solution 1, adopting a No. 20 needle, controlling spinning parameters (spinning solution flow rate is 0.3mm/min, positive high voltage +14kV, negative high voltage-3 kV, distance between a receiving device and an injection needle is 14cm, and rotating speed is 1200r/min), and spraying the spinning solution 1 onto a rotating round needle tube (inner diameter is 1.5mm) to prepare a nerve conduit inner membrane with the wall thickness of 0.15 mm; similarly, 1.6g of polylactic acid is weighed by an electronic balance to be dissolved in 10mL of organic solvent (dichloromethane: ethyl acetate volume ratio is 7:4), an electromagnetic stirrer is used for stirring for 6h to fully dissolve the polylactic acid to obtain uniform and stable polylactic acid spinning solution 2, a No. 21 needle is adopted, spinning parameters (the flow rate of the spinning solution is 0.3mm/min, positive high pressure +12kV, negative high pressure-3 kV, the distance between a receiving device and a jet needle is 15cm, and the rotating speed is 1400r/min) are controlled, the polylactic acid/fibroin spinning solution 2 is sprayed on the inner layer membrane of the catheter to prepare the outer layer membrane of the nerve catheter with the thickness of 0.35 mm; soaking the nerve conduit composed of the inner layer membrane and the outer layer membrane into a chitosan solution with the concentration of 8%, taking out after soaking for 10min, putting into a vacuum drying oven to remove residual organic solvent and moisture to obtain the polylactic acid/fibroin/chitosan composite nerve conduit, and storing in the drying oven.
FIG. 1 is a Scanning Electron Microscope (SEM) photograph (5 μm) of the inner layer membrane of the composite nerve conduit prepared by the present invention; FIG. 2 is a Scanning Electron Microscope (SEM) picture (50 μm) of the inner layer membrane of the composite nerve conduit prepared by the present invention; FIG. 3 is a Scanning Electron Microscope (SEM) photograph of the outer layer membrane of the composite nerve conduit prepared by the present invention; FIG. 4 is a photograph of the inner pore diameter of the composite nerve conduit prepared by the present invention; fig. 5 is a photograph of the composite nerve conduit prepared by the present invention. As can be seen from the figure, the nerve conduit film prepared by electrostatic spinning is well spun into a net structure, the diameter of the spun fiber is uniform, the aperture is regular, a thin chitosan film is attached to the surface of the film and well covered on the film, and the chitosan can effectively improve the cell activity and inhibit the formation of nerve scars and nerve tumors.
The polylactic acid/fibroin/chitosan composite nerve conduit prepared by the method provided by the invention is subjected to mechanical strength test, the results are shown in the following table 1, and as can be seen from the table 1, the polylactic acid/fibroin/chitosan composite nerve conduit prepared by the embodiments of the invention has certain mechanical strength.
TABLE 1 results of mechanical Strength test
| Examples | Example 1 | Example 2 | Example 3 | Example 4 |
| Strength/Mp (n ═ 5) | 5.35 | 5.67 | 6.56 | 6.87 |
TABLE 2 toxicity testing of materials
| Examples | Example 1 | Example 2 | Example 3 | Example 4 |
| Cell proliferation rate/% (n ═ 5) | 85.5 | 86.7 | 84.8 | 85.7 |
| Grade of |
1 | 1 | 1 | 1 |
The polylactic acid/fibroin/chitosan composite nerve conduits prepared in the groups of embodiments are placed in PBS solution to be soaked for one night to obtain leaching liquor, toxicity test is carried out on the leaching liquor, Xuewang cells are used for testing, the test result is shown in Table 2, the results can obtain that each material has certain cell proliferation rate, after 5 days, the cell proliferation rate is tested and calculated, the cell proliferation rate of each embodiment is over 80 percent, the corresponding material toxicity grades are all one grade, the national standard requirements of implantable bodies are met, and the polylactic acid/fibroin/chitosan composite nerve conduits have good cell affinity and biocompatibility.
It is apparent that the above embodiments are only examples for clearly illustrating and do not limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications are therefore intended to be included within the scope of the invention as claimed.
Claims (7)
1. A preparation method of a degradable polylactic acid/silk fibroin/chitosan composite nerve conduit is characterized by comprising the following steps:
(1) dissolving polylactic acid and silk fibroin in an organic solvent, electromagnetically stirring until the polylactic acid and the silk fibroin are fully dissolved to obtain a spinning solution 1, and spraying the spinning solution 1 on a rotating needle tube by an electrostatic spinning technology to prepare an inner membrane of a nerve conduit, wherein the mass ratio of the polylactic acid to the silk fibroin is 1:3 ~ 1: 6;
(2) dissolving polylactic acid in an organic solvent, electromagnetically stirring until the polylactic acid is fully dissolved to obtain a spinning solution 2, spraying the spinning solution 2 on the inner membrane of the nerve conduit obtained in the step (1) by using an electrostatic spinning technology, and preparing an outer membrane of the nerve conduit on the inner membrane of the nerve conduit, wherein the molecular weight of the polylactic acid is 60000 ~ 320000 Da;
(3) dissolving chitosan in a weakly acidic solution to obtain a chitosan solution with a certain concentration, immersing the nerve conduit consisting of the inner layer membrane and the outer layer membrane obtained in the step (2) into the chitosan solution for a period of time, taking out the nerve conduit, putting the nerve conduit into a vacuum drying oven, and removing the organic solvent to obtain the polylactic acid/fibroin/chitosan composite nerve conduit, wherein the molecular weight of the chitosan is 50000 ~ 300000Da, the thickness of the inner layer membrane of the polylactic acid/fibroin/chitosan composite nerve conduit is 0.05 ~ 0.1.1 mm, and the thickness of the outer layer membrane is 0.05 ~ 0.1.1 mm.
2. The preparation method of claim 1, wherein the inner pore size of the polylactic acid/silk fibroin/chitosan composite nerve conduit is 1.5 ~ 2 mm.
3. The process according to claim 1, wherein the electrostatic spinning in the step (1) is carried out at a spinning dope flow rate of 0.03 ~ 0.5.5 mm/min, a negative high voltage of-1 kV ~ -3kV, a positive high voltage of +7kV ~ +17kV, a take-up distance to a needle tip distance of 5 ~ 20cm, and a rotation speed of 200 ~ 2000 r/min.
4. The production method according to claim 1, wherein the mass fraction of the polylactic acid in the spinning solution 2 in the step (2) is 8% ~ 20%.
5. The process according to claim 1, wherein the electrostatic spinning in the step (2) is carried out at a spinning dope flow rate of 0.03 ~ 0.5.5 mm/min, a negative high voltage of-0.5 kV ~ -3kV, a positive high voltage of +7kV ~ +17kV, a receiving distance to a needle of 8 ~ 18cm, and a rotation speed of 200 ~ 2000 r/min.
6. The method according to claim 1, wherein the chitosan solution of step (3) has a chitosan mass concentration of 5 ~ 15%.
7. The polylactic acid/silk fibroin/chitosan compound nerve conduit prepared by the preparation method of any one of claims 1 ~ 6.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110113258A (en) * | 2010-04-09 | 2011-10-17 | 조선대학교산학협력단 | Biocomposite produced from silk fibroin powder and artificial conduit made of the same |
| CN103751839A (en) * | 2013-12-17 | 2014-04-30 | 中国人民解放军第二军医大学 | Polylactic acid-chitosan composite nerve conduit and preparation method thereof |
| CN104474589A (en) * | 2014-12-23 | 2015-04-01 | 山东国际生物科技园发展有限公司 | Guided tissue regeneration membrane as well as preparation method and application thereof |
| CN104689376A (en) * | 2015-03-17 | 2015-06-10 | 邹蓉 | Nerve conduit and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110113258A (en) * | 2010-04-09 | 2011-10-17 | 조선대학교산학협력단 | Biocomposite produced from silk fibroin powder and artificial conduit made of the same |
| CN103751839A (en) * | 2013-12-17 | 2014-04-30 | 中国人民解放军第二军医大学 | Polylactic acid-chitosan composite nerve conduit and preparation method thereof |
| CN104474589A (en) * | 2014-12-23 | 2015-04-01 | 山东国际生物科技园发展有限公司 | Guided tissue regeneration membrane as well as preparation method and application thereof |
| CN104689376A (en) * | 2015-03-17 | 2015-06-10 | 邹蓉 | Nerve conduit and preparation method thereof |
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