CN113956965B - Device for simulating waterway of oral cavity comprehensive treatment table and biofilm forming method - Google Patents
Device for simulating waterway of oral cavity comprehensive treatment table and biofilm forming method Download PDFInfo
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- 230000002572 peristaltic effect Effects 0.000 claims abstract description 56
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- 229920003023 plastic Polymers 0.000 claims abstract description 47
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 20
- 230000032770 biofilm formation Effects 0.000 claims abstract description 13
- 229910052742 iron Inorganic materials 0.000 claims abstract description 10
- 239000000741 silica gel Substances 0.000 claims abstract description 10
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 6
- -1 polypropylene Polymers 0.000 claims abstract description 5
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 4
- 238000005070 sampling Methods 0.000 claims description 43
- 238000004088 simulation Methods 0.000 claims description 22
- 230000008569 process Effects 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 17
- 230000015572 biosynthetic process Effects 0.000 claims description 11
- 239000008399 tap water Substances 0.000 claims description 9
- 235000020679 tap water Nutrition 0.000 claims description 9
- 239000008213 purified water Substances 0.000 claims description 8
- 239000008223 sterile water Substances 0.000 claims description 8
- 230000006855 networking Effects 0.000 claims description 6
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- 238000002474 experimental method Methods 0.000 claims description 3
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- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 abstract 1
- 239000004743 Polypropylene Substances 0.000 abstract 1
- 238000007599 discharging Methods 0.000 abstract 1
- 229920001155 polypropylene Polymers 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 229920001897 terpolymer Polymers 0.000 abstract 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention provides a device for simulating a waterway of an oral cavity comprehensive treatment table and a biofilm formation method, wherein the device comprises: a water inlet tank; a silica gel connecting pipe; an intelligent timing control box; a peristaltic pump; a straight-through joint made of polypropylene; a plastic waterway pipeline made of polytetrafluoroethylene; a three-way valve made of plastic material and composed of terpolymer of acrylonitrile, butadiene and styrene; discharging the water tank; an experimental iron stand; 1ml syringe. The biofilm formation method comprises the following steps: s1, respectively introducing water of different water sources into four water inlet tanks; s2, setting a biological film observing interval at a small program end; s3, running the device. The invention solves the problem that the waterway pipeline of the actual oral cavity comprehensive treatment table is not easy to detach so as to observe the growth cycle and growth characteristics of the biological film, and provides scientific research method and disinfection basis for the waterway pollution problem of the oral cavity comprehensive treatment table.
Description
Technical Field
The invention relates to the technical field of bacterial biofilm incubation systems, in particular to a device for simulating a waterway of an oral cavity comprehensive treatment table and a biofilm formation method.
Background
The problem of waterway pollution of the oral cavity comprehensive treatment table is common worldwide, and the health and safety of patients and medical staff are seriously threatened. Research for many years proves that the waterway of the oral cavity comprehensive treatment table has serious pollution, and the microorganism in each milliliter of water sample is up to 10 percent without treatment of the waterway 8 CFU contains conditional pathogenic bacteria such as Pseudomonas aeruginosa and Legionellosis pneumophila. When the pathogenic microorganisms are used for oral treatment, the pathogenic microorganisms can enter wounds of patients along with rotation of the mobile phone and water mist sprayed by the three-purpose gun, so that nosocomial infection is caused. The effect is especially important for patients with hypoimmunity such as pregnant women, children, malignant tumor chemotherapy, and people taking immunosuppressant and people with immunity deficiency such as acquired immunodeficiency syndrome. Researches show that the problem of waterway pollution of the oral cavity comprehensive treatment table is difficult to effectively solve and is mainly influenced by biological membranes in waterway pipelines. The biological film in waterway is different from the growth mode of free bacteria, it is a complex bacterial combination, and is a microbial membranous polymer adhered to the surface of some living or non-living carrier, and the main components of said polymer include protein, saccharide substance, lipid substance, extracellular DNA, etc. Because the diameter of the waterway pipeline of the dental chair is small, the liquid flows slowly, stops intermittently, and the high-speed mobile phone instantly sucks back negative pressure to cause pollution, and the like, a biomembrane is easy to generate in the pipeline. Once formed, the biofilm has sessile cells excrete polysaccharide-protein complexes with high viscoelasticity, making them difficult to remove in dental chair waterways and resistant to desiccation and self-protection. During dental treatment, biofilm adhering to the surface of the tubing may constantly release the conditionally pathogenic bacteria into the waterway, which, along with the water flow, enter the treatment site, causing cross-infection of the dental treatment, which may be caused to shock by severe persons. Therefore, effective removal of the biofilm, particularly prevention of biofilm formation in waterway pipes, is critical in controlling the occurrence of infection caused by dental chair waterways. However, the research on biological membranes is limited due to the high cost of the dental chair and the inconvenient detachment. The method for forming biological film by simulating the waterway of the oral cavity comprehensive treatment table is mainly based on static culture method at present, and oral cavity comprehensive treatment is performedThe waterway of the therapy table is in a dynamic and static continuously-changing state and is influenced by the back suction of the high-speed mobile phone, and the existing static culture method cannot be close to the real growth environment, so that judgment is influenced.
At present, little research is done on dynamic simulation devices and methods for forming waterway biofilms of oral cavity comprehensive treatment tables, and only patents for forming waterway pipelines mainly focus on tap water supply.
1. The invention discloses a simulation experiment device and a simulation experiment method (application number: 202010691936.9) for the growth of a biological film on the wall of a long water pipeline, and the invention discloses the simulation experiment device for the growth of the biological film on the wall of the long water pipeline, although the method can simulate a multi-stage water pipeline network; the growth process of the biological film on the pipe wall of the long-distance water conveying pipe under the conditions of different lengths and different residence time is effectively simulated, but due to the complex conditions in the dental treatment process, the method cannot consider the conditions of waterway circulation working time, high-speed handset back suction and the like, cannot be well close to clinical use conditions, and is different from a research object of the research.
2. A biomembrane growth simulator for water supply pipeline with hanging plate on its inner surface is disclosed (application number: 202110108547.3), which has high stability, more hanging plate positions and can simulate pipeline flow state.
3. The invention discloses a reactor for in-situ culturing pipeline biological film and a method thereof (application number: 202110231901.1), and discloses a reactor for in-situ culturing pipeline biological film and a method thereof.
Therefore, the invention provides a device for simulating the formation of the waterway biological film of the oral cavity comprehensive treatment table and a method for forming the biological film, and aims to obtain the biological film by simulating the construction of the device for simulating the formation of the waterway biological film of the oral cavity comprehensive treatment table and the fumbling of the method for forming the biological film, so that the subsequent study on the formation characteristics of the biological film and the study on the removal effect of different disinfectants on the biological film are convenient.
Disclosure of Invention
The invention aims to provide a device for simulating the formation of a waterway biological film of an oral cavity comprehensive treatment table and a method for forming the biological film, wherein the peristaltic pump is used for intelligently controlling the flow rate of water flow in a plastic pipeline, and simultaneously, the three-way valve is used for injecting oral cavity liquid in a dental treatment of a patient to simulate the back suction of a high-speed mobile phone, so that the simulation device is ensured to be maximally close to the process of forming the biological film in an actual waterway; the PLC is used for acquiring the holding amounts of different water sources in the water inlet tank, the working state of the peristaltic pump and the generation time of the biological film in real time, and a sampling module is provided. The invention provides a reliable novel device and a method for producing a biological film, which solve the problem that an actual waterway pipeline is not easy to detach and obtain the biological film. The biological film obtained by the device and the method can be used for conveniently researching the removal effect of different disinfectants on the biological film, and has stronger practical value.
The technical scheme of the invention is as follows:
a device for simulating a waterway of an oral cavity comprehensive treatment table, comprising:
a simulation device for simulating the waterway working process of the oral cavity comprehensive treatment table,
the small program end is used for acquiring the working state of the simulation equipment and the biofilm formation state through the mobile end,
wherein the simulation device comprises:
a water supply module for simulating different water sources of treatment platform water route water supply function, water supply module includes: four water inlet tanks for storing source water, pressure sensors are arranged at the bottoms of the water inlet tanks, the four water inlet tanks are connected with peristaltic pumps through silica gel connecting pipes, the peristaltic pumps are electrically connected with the intelligent timing control box, the water outlets of the peristaltic pumps are respectively connected with four plastic waterway pipelines through joints, the other ends of the four plastic waterway pipelines are respectively connected with three-way valves, the four three-way valves are also respectively connected with a discharge water tank and a 1mL syringe for imitating clinical high-speed mobile phone to suck back liquid in the oral cavity of a patient,
four plastics waterway pipelines are close to three way valve one end and realize the fixed of horizontal direction through experimental iron stand platform respectively, are equipped with the sampling module that is convenient for take a sample the observation to the biomembrane on the plastics waterway pipeline respectively, and the position at one of them experimental iron stand platform middle part is fixed with control module, is equipped with in the control module: a PLC controller electrically connected with the intelligent timing control box and the four pressure sensors, a wireless networking sub-module electrically connected with the PLC controller and used for connecting with the small program end,
the applet end comprises: the peristaltic pump state module is used for acquiring and displaying working time and working state of the peristaltic pump at regular time, the time period setting module is used for setting a biological film sampling observation time interval, the prompting module is used for prompting a user to check the biological film state of the water inlet tank, the peristaltic pump or different sampling time through ringing or vibration, and the biological film state recording module is used for recording the biological film state of sampling at each time interval.
Further, the prompt module includes: the peristaltic pump state prompting sub-module is used for prompting a user to check and confirm the working state of the peristaltic pump, the biomembrane state prompting sub-module is used for prompting the user according to the time interval set by the time interval setting module, and the syringe prompting sub-module is used for prompting the user to sample through a 1mL syringe at the time interval of 30 min.
Further, the sampling module includes: cutting the second plastics water route pipeline so as to be convenient for turn out the sample district of internal surface, sample district a week is equipped with the sealing washer that is used for preventing to leak, and sample district one side is connected with the fixture block, and second plastics water route pipeline corresponds the position with the fixture block and is equipped with the draw-in groove, and sampling module's design is convenient for device user to the acquisition of biofilm formation state in different time periods for device user can obtain the data of biofilm more, convenient to use.
Further, the front surface of the control module is provided with a touch display screen for displaying the type and the residual amount of the water source in each water inlet tank, recording and displaying the working state and the working time of the peristaltic pump, calculating and displaying the forming time of the biological film and the sampling times of the biological film, so that the device is convenient for a device user to observe the state of the simulation equipment.
Furthermore, the bottom end of the water inlet tank is higher than the top of the water discharge tank, so that water flow in the plastic waterway pipeline can be kept in a stagnation state, and generation of a biological film is facilitated.
Preferably, the plastic waterway pipeline is made of plastic materials made of polytetrafluoroethylene materials, the diameter is 3 x 2mm, and the diameter is consistent with the diameter of the waterway pipeline material of the actual oral cavity comprehensive treatment table, so that the simulation equipment can be matched with the actual situation as much as possible, the plastic waterway pipeline is fixed in a u-shaped manner through the experimental iron stand table, and the plastic waterway pipeline is highly close to the placement state of the clinical waterway pipeline.
Preferably, the water sources of the four water inlet tanks are respectively: purified water, domestic tap water, water treated by a central water supply system and sterile water, and various water can provide more data for the biofilm generation process, so that the device is convenient for the research of device users.
The invention also provides a biomembrane forming method for simulating the waterway of the oral cavity comprehensive treatment table based on the device, which comprises the following steps:
s1, respectively introducing purified water, domestic tap water and water treated by a central water supply system and sterile water into four water inlet tanks;
s2, a user logs in a small program end, and a biological film sampling observation time interval is set through a time period setting module;
s3, starting simulation equipment, wherein the PLC controls the peristaltic pump to work through the intelligent timing control box, in the working process of the peristaltic pump, a user samples and observes a biomembrane generated by the plastic waterway pipeline through the sampling module according to a set time interval, records an observation result through the biomembrane state recording module, and changes all the plastic waterway pipelines after the observation is finished.
Further preferably, the specific working process of the simulation device in step S3 is: the peristaltic pump simultaneously controls the liquid in the four water inlet tanks to flow into the four plastic waterway pipelines after being sucked out through the silica gel connecting pipe, the water flow speed is 60ml/min, the working time is 8:00 to 17:00, the liquid stops for 10 minutes after each half hour of working, the non-working time keeps the water flow stop state, and when the working is stopped for each half hour, 100ul of oral liquid in dental treatment of a patient is instantly injected from the three-way valve through the 1ml syringe so as to simulate the high-speed mobile phone suck-back.
The four water inlet tanks are all 5L of sealed and capped opaque glass bottles, the discharge water tank is 5L of sealed and capped opaque glass bottles, the flow of the 24h waterway pipeline can be ensured, the bottom of the water inlet tank is 0.2m higher than the top of the discharge water tank, and the raw water is replaced once for 24 h.
The beneficial effects of the invention are as follows:
1. the invention provides a device for simulating the formation of a waterway biological film of an oral cavity comprehensive treatment table and a method for forming the biological film, wherein the peristaltic pump is intelligently controlled to adjust the flow rate of water flow so as to simulate the dynamic change state of the waterway of the oral cavity comprehensive treatment table; the liquid in the oral cavity is injected into the dental treatment of the patient through the three-way valve to simulate the high-speed handset to suck back; the u-shaped fixation of the plastic waterway pipeline is realized through the experimental iron stand, the placement state of the clinical waterway pipeline is simulated, and the process that the simulation device is close to the formation of a biological film in an actual waterway is ensured to the greatest extent;
2. according to the device for simulating the formation of the waterway biological film of the oral cavity comprehensive treatment table and the method for forming the biological film, provided by the invention, the holding amounts of different water sources in the water inlet tank, the working state of the peristaltic pump and the time for generating the biological film are obtained in real time through the PLC, the sampling module is provided, and the problem that an actual waterway pipeline is difficult to detach and obtain the biological film is solved;
3. the device for simulating the formation of the waterway biological film of the oral cavity comprehensive treatment table and the method for forming the biological film can be used for sampling at any time in the biological film formation process, so that the biological films in different growth stages can be obtained, and the subsequent research on the biological films is facilitated;
4. the device for simulating the formation of the waterway biological film of the oral cavity comprehensive treatment table and the method for forming the biological film can directly put the disinfectant into the water inlet tank, and facilitate the subsequent research on the removal effect of the disinfectant on the biological film.
Drawings
FIG. 1 is a frame diagram of the present invention;
FIG. 2 is a block diagram of an analog device of the present invention;
FIG. 3 is an enlarged detail view at A in FIG. 2;
FIG. 4 is a diagram of the internal architecture of the control module of the present invention;
FIG. 5 is a block diagram of a prompt module of the present invention;
the device comprises a 1-water inlet tank, a 2-silica gel connecting pipe, a 3-intelligent timing control box, a 4-peristaltic pump, a 5-straight joint, a 6-plastic waterway pipeline, a 7-experiment iron stand, an 8-three-way valve, a 9-discharge water tank, a 10-1ml injector, a 11-sampling module, a 12-control module, a 13-PLC (programmable logic controller), a 14-wireless networking sub-module, a 15-sampling area, a 16-sealing ring, a 17-clamping block, an 18-clamping groove, a 19-touch display screen and a 20-pressure sensor.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The terminology used in the embodiments of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise, the "plurality" generally includes at least two.
Example 1
As shown in fig. 1, this embodiment is an apparatus for simulating a waterway of an oral cavity comprehensive treatment table, including:
a simulation device for simulating the waterway working process of the oral cavity comprehensive treatment table,
the small program end is used for acquiring the working state of the simulation equipment and the biofilm formation state through the mobile end,
as shown in fig. 2, wherein the simulation device comprises:
a water supply module for simulating different water sources of treatment platform water route water supply function, water supply module includes: four water inlet tanks 1 for storing source water, and the water sources of the four water inlet tanks 1 are respectively: purified water, domestic tap water and water treated by a central water supply system and sterile water, wherein a pressure sensor 20 is arranged at the bottom of the water inlet tank 1, four water inlet tanks 1 are connected with peristaltic pumps 4 through silica gel connecting pipes 2, the peristaltic pumps 4 are electrically connected with intelligent timing control boxes 3, water outlets of the peristaltic pumps 4 are respectively connected with four plastic waterway pipelines 6 through joints 5, the other ends of the four plastic waterway pipelines 6 are respectively connected with three-way valves 8, the four three-way valves 8 are respectively connected with a water discharge tank 9 and a 1mL injector 10 for imitating the clinical high-speed mobile phone to suck back liquid in the oral cavity of a patient, the bottom end of the water inlet tank 1 is higher than the top of the water discharge tank 9, the plastic waterway pipelines 6 are made of polytetrafluoroethylene plastic materials,
four plastics waterway pipes 6 are close to tee bend valve 8 one end and realize the fixed of horizontal direction through experimental iron stand 7 respectively, and the position at one of them experimental iron stand 7 middle part is fixed with control module 12, is equipped with in the control module 12: the PLC 13 is electrically connected with the intelligent timing control box 3 and the four pressure sensors 20, the wireless networking sub-module 14 is electrically connected with the PLC 13 and used for connecting a small program end, the touch display screen 19 is arranged on the front surface of the control module 12 and used for displaying the type and the residual amount of the built-in water source of each water inlet box 1, recording and displaying the working state and the working time of the peristaltic pump 4, calculating and displaying the biofilm formation time and calculating and displaying the biofilm sampling times,
the applet end comprises: the peristaltic pump state module is used for acquiring and displaying the working time and the working state of the peristaltic pump 4 at regular time, the time period setting module is used for setting a biological film sampling observation time interval, the prompting module is used for prompting a user to check the biological film state of the water inlet tank 1, the peristaltic pump 4 or different sampling times through ringing or vibration, and the biological film state recording module is used for recording the biological film state of sampling at each time interval.
Example 2
This example is a biofilm formation method of a device for simulating a waterway of an oral integrated treatment table based on the device of example 1, comprising the steps of:
s1, respectively introducing purified water, domestic tap water and water treated by a central water supply system and sterile water into four water inlet tanks 1;
s2, a user logs in a small program end, and a biological film sampling observation time interval is set through a time period setting module;
s3, simulating equipment is started, the PLC 13 controls the peristaltic pump 4 to work through the intelligent timing control box 3, in the working process of the peristaltic pump 4, a user samples and observes the biological film generated by the plastic waterway pipeline 6 through the sampling module 11 according to a set time interval, the observation result is recorded through the biological film state recording module, and after the observation is finished, all the plastic waterway pipelines 6 are replaced.
The specific working process of the simulation device in the step S3 is as follows: the peristaltic pump 4 simultaneously controls the liquid in the four water inlet tanks 1 to flow into the four plastic waterway pipelines 6 after being sucked out through the silica gel connecting pipe 2, the water flow speed is 60ml/min, the working time is 8:00 to 17:00, the water is stopped for 10min after each half hour of working, the water is kept in a water flow stopping state in the non-working time, and when the working is stopped for each half hour, 100ul of oral liquid in the dental treatment of a patient is instantly injected from the three-way valve 8 through the 1ml syringe 10 so as to simulate the high-speed handset suck-back.
Example 3
As shown in fig. 5, this embodiment differs from embodiment 1 in that:
the prompt module comprises: the peristaltic pump state prompting sub-module is used for prompting a user to check and confirm the working state of the peristaltic pump 4, the biomembrane state prompting sub-module is used for prompting the user according to the time interval set by the time interval setting module, and the syringe prompting sub-module is used for prompting the user to sample through the 1mL syringe 10 at the time interval of 30 min.
Example 4
This embodiment is a biofilm formation method of a device for simulating a waterway of an oral integrated treatment table according to one embodiment 3, comprising the steps of:
s1, respectively introducing purified water, domestic tap water and water treated by a central water supply system and sterile water into four water inlet tanks 1;
s2, a user logs in a small program end, and a biological film sampling observation time interval is set through a time period setting module;
s3, simulating equipment is started, a PLC (programmable logic controller) 13 controls a peristaltic pump 4 to work through an intelligent timing control box 3, when the peristaltic pump 4 cannot be controlled by the PLC 13 through the intelligent timing control box 3 in the working process of the peristaltic pump 4, the state of the peristaltic pump 4 is transmitted to a small program end through a wireless networking sub-module 14, a peristaltic pump state prompting sub-module prompts a user to overhaul the peristaltic pump 4, when a set biological film observing time is reached, the biological film state prompting sub-module prompts the user to observe the biological film through a sampling module 11, the user observes the biological film generated by a plastic waterway pipeline 6 through the sampling module 11 according to a set time interval, the observation result is recorded through a biological film state recording module, and after the observation is ended, all the plastic waterway pipelines 6 are replaced.
The specific working process of the simulation device in the step S3 is as follows: the peristaltic pump 4 simultaneously controls the liquid in the four water inlet tanks 1 to flow into the four plastic waterway pipelines 6 after being sucked out through the silica gel connecting pipe 2, the flow speed of the water is 60ml/min, the working time is 8:00-17:00, the water is stopped for 10min after each half hour of working, the non-working time is kept in a water flow stopping state, and when the working is stopped for each half hour, the injector reminding submodule immediately prompts a user to inject 100ul of oral liquid in dental treatment of a patient from the three-way valve 8 through the 1ml injector 10 so as to simulate high-speed mobile phone suck-back.
Example 5
As shown in fig. 3, this embodiment differs from embodiment 3 in that:
the sampling module 11 includes: the plastic waterway pipeline 6 is cut so as to conveniently turn out a sampling area 15 on the inner surface, a sealing ring 16 for preventing water leakage is arranged on one circle of the sampling area 15, a clamping block 17 is connected to one side of the sampling area 15, and a clamping groove 18 is arranged at a position of the plastic waterway pipeline 6 corresponding to the clamping block 17.
Example 6
This embodiment is a biofilm formation method of a device for simulating a waterway of an oral integrated treatment table according to one embodiment of the device of embodiment 5, comprising the steps of:
s1, respectively introducing purified water, domestic tap water and water treated by a central water supply system and sterile water into four water inlet tanks 1;
s2, a user logs in a small program end, and a biological film sampling observation time interval is set through a time period setting module;
s3, simulating equipment is started, a PLC (programmable logic controller) 13 controls a peristaltic pump 4 to work through an intelligent timing control box 3, when the peristaltic pump 4 cannot be controlled by the PLC 13 through the intelligent timing control box 3 in the working process of the peristaltic pump 4, the state of the peristaltic pump 4 is transmitted to a small program end through a wireless networking sub-module 14, a peristaltic pump state prompting sub-module prompts a user to overhaul the peristaltic pump 4, when a set biological film observing time is reached, the biological film state prompting sub-module prompts the user to observe the biological film through a sampling module 11, the user observes the biological film generated by a plastic waterway pipeline 6 through the sampling module 11 according to a set time interval, the observation result is recorded through a biological film state recording module, and after the observation is ended, all the plastic waterway pipelines 6 are replaced.
The specific working process of the simulation device in the step S3 is as follows: the peristaltic pump 4 simultaneously controls the liquid in the four water inlet tanks 1 to flow into the four plastic waterway pipelines 6 after being sucked out through the silica gel connecting pipe 2, the flow speed of the water is 60ml/min, the working time is 8:00-17:00, the water is stopped for 10min after each half hour of working, the non-working time is kept in a water flow stopping state, and when the working is stopped for each half hour, the injector reminding submodule immediately prompts a user to inject 100ul of oral liquid in dental treatment of a patient from the three-way valve 8 through the 1ml injector 10 so as to simulate high-speed mobile phone suck-back.
In step S3, the specific process of sampling and observing the biological film is as follows: the sampling area 15 is lifted by the device user through the clamping block 17, the biomembrane formed in the inner surface of the plastic waterway pipeline 6 is displayed, and the device user takes out part of the biomembrane for observation.
Claims (8)
1. A device for simulating a waterway of an oral cavity comprehensive treatment table, comprising:
a simulation device for simulating the waterway working process of the oral cavity comprehensive treatment table,
the small program end is used for acquiring the working state of the simulation equipment and the biological film forming state through the mobile end,
wherein the simulation device comprises:
a water supply module for simulating different water sources of treatment table water route water supply function, water supply module includes: four water inlet tanks (1) for storing source water, a pressure sensor (20) is arranged at the inner bottom of each water inlet tank (1), each water inlet tank (1) is connected with a peristaltic pump (4) through a silica gel connecting pipe (2), each peristaltic pump (4) is electrically connected with an intelligent timing control box (3), each peristaltic pump (4) water outlet is connected with four plastic waterway pipelines (6) through a straight-through joint (5), the other ends of the four plastic waterway pipelines (6) are respectively connected with a three-way valve (8), each three-way valve (8) is also respectively connected with a water outlet tank (9) and a 1mL syringe (10) for imitating clinical high-speed mobile phone back-sucking liquid in the oral cavity of a patient,
four plastics waterway pipeline (6) are close to three way valve (8) one end and realize fixed of horizontal direction through experiment iron stand platform (7) respectively, are equipped with sampling module (11) that are convenient for take a sample and observe the biomembrane on plastics waterway pipeline (6) respectively, one of them the position at experiment iron stand platform (7) middle part is fixed with control module (12), be equipped with in control module (12): a PLC controller (13) electrically connected with the intelligent timing control box (3) and the four pressure sensors (20), a wireless networking sub-module (14) electrically connected with the PLC controller (13) and used for connecting the small program end,
the sampling module (11) comprises: the plastic waterway pipeline (6) is cut so as to conveniently turn out a sampling area (15) on the inner surface, a sealing ring (16) for preventing water leakage is arranged on one circle of the sampling area (15), a clamping block (17) is connected to one side of the sampling area (15), a clamping groove (18) is arranged at the position of the plastic waterway pipeline (6) corresponding to the clamping block (17),
the applet end comprises: the peristaltic pump comprises a peristaltic pump state module for acquiring and displaying working time and working state of the peristaltic pump (4) at regular time, a time period setting module for setting a biological film sampling observation time interval, a prompting module for prompting a user to check biological film states of the water inlet tank (1), the peristaltic pump (4) or different sampling times through ringing or vibration, a biological film state recording module for recording the biological film state of sampling at each time interval, and a water residual amount monitoring module for displaying and monitoring water amount in the water inlet tank (1).
2. The apparatus for simulating a waterway of an oral cavity treatment table of claim 1, wherein the prompting module comprises: the peristaltic pump state prompting sub-module is used for prompting a user to check and confirm the working state of the peristaltic pump (4), the biomembrane state prompting sub-module is used for prompting the user according to the time interval set by the time interval setting module, and the injector prompting sub-module is used for prompting the user to sample through the 1mL injector (10) at the time interval of 30 min.
3. The device for simulating the waterway of the oral cavity comprehensive treatment table according to claim 1, wherein the control module (12) is provided with a touch display screen (19) on the front surface for displaying the type of the built-in water source of each water inlet tank (1) and the residual amount thereof, recording and displaying the working state and the working time of the peristaltic pump (4), calculating and displaying the formation time of the biological film and calculating and displaying the sampling times of the biological film.
4. A device for simulating the waterway of an oral cavity comprehensive treatment table according to claim 1, wherein the bottom end of the water inlet tank (1) is higher than the top of the discharge water tank (9).
5. A device for simulating the waterway of an oral cavity comprehensive treatment table according to claim 1, wherein the plastic waterway pipeline (6) is made of polytetrafluoroethylene plastic material.
6. The device for simulating the waterway of the oral cavity comprehensive treatment table according to claim 1, wherein the water sources of the four water inlet tanks (1) are respectively: purified water, tap water, water treated by a central water supply system and sterile water.
7. A method of biofilm formation in a device for simulating a waterway of an oral complex treatment table according to any one of claims 1 to 6, comprising the steps of:
s1, respectively introducing purified water, domestic tap water and water treated by a central water supply system and sterile water into four water inlet tanks (1);
s2, a user logs in a small program end, and a biological film sampling observation time interval is set through a time period setting module;
s3, simulating equipment is started, a PLC (13) controls a peristaltic pump (4) to work through an intelligent timing control box (3), in the working process of the peristaltic pump (4), a user samples and observes a biomembrane generated by a plastic waterway pipeline (6) through a sampling module (11) according to a set time interval, an observation result is recorded through a biomembrane state recording module, and after the observation is finished, all the plastic waterway pipelines (6) are replaced.
8. The biofilm formation method for simulating a waterway of an oral cavity comprehensive treatment table according to claim 7, wherein the specific working process of the simulation device in the step S3 is as follows: the peristaltic pump (4) simultaneously controls the liquid in the four water inlet tanks (1) to flow into the four plastic waterway pipelines (6) after being sucked out through the silica gel connecting pipe (2), the flow speed of the water flow is 60ml/min, the working time is 8:00-17:00, the water flow is stopped for 10min after each half hour of working, the water flow is kept in a stopped state during the non-working time, and when the working is stopped for each half hour, 100ul of oral liquid in the dental treatment of a patient is injected from the three-way valve (8) through the 1ml syringe (10) so as to simulate the high-speed mobile phone suck-back.
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