CN113855620A - Composition containing saussurea involucrate cell culture and preparation method and application thereof - Google Patents
Composition containing saussurea involucrate cell culture and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an anti-inflammatory and/or whitening composition and a preparation method and application thereof, and relates to the field of cosmetics. The anti-inflammatory and/or whitening composition comprises the following raw materials: the mass ratio of the saussurea involucrate cell culture to the schisandra chinensis is 1:3-4: 1; the preparation method of the composition comprises the following steps: s1, mixing the saussurea involucrate cell culture, schisandra chinensis and 1, 3-butanediol, and carrying out hot reflux extraction; s2, fine filtering and then compounding resveratrol to obtain the composition; the application refers to the application of the composition in preparing anti-inflammatory and/or whitening products. The composition provided by the invention is reasonably proportioned, synergistically enhances the effects of whitening, anti-inflammation and regulating antibacterial peptide, is applied to a cosmetic formula, can remarkably enhance the anti-inflammation and whitening effects, is prepared from natural medicinal plants verified by years of clinical tests, is high in biological safety, is environment-friendly and has a wide application prospect.
Description
Technical Field
The invention relates to the field of cosmetics, in particular to a composition containing a saussurea involucrate cell culture, a preparation method thereof and application thereof in cosmetics.
Background
From the medical point of view, the inflammatory response is the basic response of human tissues to the stimulation of various injury factors, mainly the defense of the human body. The causes of skin inflammation mainly include the following aspects: 1. external environment stimulation: the health condition of the skin is influenced by various factors such as the external environment. In common environments with supercooling or overheating, strong wind sand and pollutants, dust, pollen and the like in the air can cause skin irritation. 2. Irritating ingredients in skin care products: such as alcohol, perfume, preservative, etc., and active ingredients in skin care products are also part of the stimulating component. The active ingredient is reversible in the cosmetic, and a moderate concentration of the active ingredient can help to improve the skin condition, and the concentration is not suitable and can cause skin inflammation due to skin intolerance. 3. Skin oil production: the skin problems of closed comedo, acne and the like are easily caused by facial skin infection due to abnormal proliferation of flora such as propionibacterium acnes, staphylococcus aureus, malassezia and the like caused by the exuberant secretion of grease. 4. Ultraviolet ray stimulation: ultraviolet short-wave UVB can directly cause cells such as skin keratinocytes and the like to release inflammatory factors, so that the skin is reddened and heated in a short time. At the same time, ultraviolet light can stimulate melanocyte activation, resulting in skin pigmentation. In addition, ultraviolet rays can accelerate skin aging and affect the normal metabolism of the skin.
Because some components in the natural plants have the effects of resisting inflammation, resisting oxidation and the like, the natural plants have mild effect on the skin, small irritation and high safety, and therefore, the natural plant components have wide application prospect in cosmetics.
Saussurea involucrate: nature and taste: bitter, slightly bitter and warm; the functional indications are as follows: clearing away heat and toxic material, dispelling pathogenic wind and dampness, relieving swelling and pain. Can be used for treating head wound, gynecological diseases, rheumatoid arthritis, apoplexy, altitude sickness, and swelling due to topical application (Xinjiang Chinese herbal medicine, Xinjiang Uygur autonomous region Committee of revolution health, 1976). The saussurea involucrate is grown on a high mountain grass slope with the elevation of about 4000 meters in the mountain range of the Tianshan mountain, on a cliff steep wall and in an ice-affected rock seam, and the saussurea involucrate has the capability of absorbing ultraviolet rays because the saussurea involucrate grows in a special environment near the snow mountain and is irradiated by the extremely strong ultraviolet rays. It is a unique and rare Chinese herbal medicine in Xinjiang. Saussurea involucrate is the only saussurea involucrate plant listed in the book of Chinese plant Red skin. The saussurea involucrate cell culture is purple red mass particles obtained by selecting the isolated tissue of the saussurea involucrate of the Compositae, taking the callus formed by dedifferentiation as a subculture seed and performing subculture under certain conditions.
Schisandra chinensis is a dried mature fruit of Schisandra chinensis (Turcz.) Baill) of Schisandra of Magnoliaceae, is a well-known traditional Chinese medicine, and also plays a role in modern medicine. Modern medical research shows that the schisandra chinensis contains a plurality of functional components such as schisandrin B, triterpenes, lignans, polysaccharides and the like, and has the activities of reducing blood sugar, resisting oxidation, protecting liver and the like. Patent CN200910069813.5 discloses application of schisandra chinensis extract in preparing sunscreen skin care products, which proves that schisandra chinensis extract has the efficacy of absorbing ultraviolet rays and can reduce inflammatory reaction caused by ultraviolet rays.
The inflammation reaction is a stress reaction of skin to external stimulation, and the inflammation may aggravate the problems of skin pigmentation, skin aging acceleration and the like, so that the development of the plant anti-inflammatory extract which has high safety, is environment-friendly, has obvious anti-inflammation and clear action mechanism has wide application market.
Disclosure of Invention
The invention provides an anti-inflammatory composition containing saussurea involucrate cell culture, a preparation method thereof and application thereof in the field of cosmetics, aiming at the problems of poor safety of anti-inflammatory action raw materials, low effect and the like in the prior art in the field of cosmetics. The anti-inflammatory composition disclosed by the invention is prepared by selecting and compounding the saussurea involucrate cell culture and the schisandra chinensis, and can synergistically play roles of enhancing the anti-inflammatory effect, regulating and controlling the antibacterial peptide, resisting oxidation and whitening, so that the anti-inflammatory composition is applied to cosmetics and the product effect is improved.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
on one hand, the invention discloses an anti-inflammatory composition, which comprises the following traditional Chinese medicine raw materials: herba Saussureae Involueratae cell culture and fructus Schisandrae chinensis.
Preferably, the mass ratio of the saussurea involucrate cell culture to the schisandra chinensis extract is 1:3-4:1, and further preferably, the mass ratio is 1: 1.
Preferably, the composition further comprises resveratrol.
Preferably, the resveratrol accounts for 0.1-0.3% by mass percent.
The saussurea involucrate cell culture, the schisandra chinensis and the resveratrol in the composition are obtained by screening a large number of plant extracts through cell experiments, and the three are determined to have the effects of inhibiting an inflammatory factor prostaglandin E2(PGE-2) and regulating and controlling an antibacterial peptide LL-37.
Saussurea involucrata, also known as snow lotus, belongs to the plant of the genus Saussurea (Saussurea) of the family Compositae, and is widely used by minority nationalities in Tibet, Xinjiang and the like as a long-history ethnic medicine. Saussurea involucrate can independently live under the harsh natural conditions of high mountains, and is closely related to a special adaptation mechanism and active ingredients formed by the saussurea involucrate. Research shows that the active components of saussurea involucrate mainly comprise polysaccharides, flavonoids, alkaloids, sterols, volatile oil and the like, and the active components enable the saussurea involucrate to have the biological functions of resisting inflammation, resisting radiation, eliminating free radicals, resisting bacteria, resisting viruses and the like. After UVB irradiation, excessive inflammatory factors in the skin activate physiological processes and the like, so that the active ingredients of the snow lotus herb have a great effect on resisting skin damage caused by UVB irradiation. The content of active ingredients of the saussurea involucrate culture is higher than that of the active ingredients of the natural plant extract by more than several times. The total flavone is 4 times higher, and the total protein is nearly 2 times higher. Compared with wild saussurea involucrate, the saussurea involucrate culture has higher content of main active ingredients.
Schisandra chinensis is a dried mature fruit of Schisandra chinensis (Turcz.) Baill) of Schisandra of Magnoliaceae, is a well-known traditional Chinese medicine, and also plays a role in modern medicine. Modern medical research shows that the schisandra chinensis contains a plurality of functional components such as schisandrin B, triterpenes, lignans, polysaccharides and the like, and has the activities of reducing blood sugar, resisting oxidation, protecting liver and the like.
Resveratrol is a plant polyphenol, also known as resveratrol. In recent years, attention has been paid to the wide range of biological activities, and numerous studies have confirmed that resveratrol has functions of resisting oxidative stress, resisting inflammation, resisting bacteria, regulating fat deposition, and the like.
On the other hand, the invention also discloses a preparation method of the composition, which comprises the following steps:
s1, mixing the saussurea involucrate cell culture, schisandra chinensis and 1, 3-butanediol, carrying out hot reflux extraction, carrying out fine filtration, and collecting filtrate to obtain an extracting solution;
s2, adding resveratrol into the extracting solution obtained in the step S1 to obtain the composition.
Preferably, the volume percentage of the 1, 3-butanediol solution in step S1 is 10-50%, preferably 30%.
Preferably, in the step S1, the ratio of the saussurea involucrate and the schisandra chinensis to the 1, 3-butanediol is 1:40-60, preferably 1: 50.
in another aspect, the invention provides the application of the composition in preparing products for resisting inflammation, whitening, resisting oxidation and/or regulating antibacterial peptide.
In still another aspect, the present invention provides an anti-inflammatory, whitening, antioxidant and/or antimicrobial peptide modulating product comprising the above composition.
Preferably, the product is a cosmetic product.
Preferably, the cosmetic includes, but is not limited to, cream, lotion, essence, makeup cream, makeup milk, BB cream, foundation, shampoo, conditioner, or toner for skin care.
In still another aspect, the present invention provides the use of the above composition in anti-inflammatory, whitening, antioxidant and/or modulating antimicrobial peptides.
Compared with the prior art, the invention has the following beneficial effects:
1. the raw materials of the anti-inflammatory composition disclosed by the invention are natural medicinal plant components, and are all natural medicinal plants which are clinically verified for many years, so that the anti-inflammatory composition is high in biological safety and environment-friendly.
2. After a large number of natural medicinal plant extracts are screened through a cell experiment, the invention determines that two natural medicinal plant components of the saussurea involucrate and the schisandra chinensis have the effects of inhibiting an inflammation factor prostaglandin E2(PGE-2) and regulating and controlling the antibacterial peptide LL-37, and the anti-inflammatory, the antibacterial peptide regulating and controlling, the antioxidant and the whitening effects can be synergistically enhanced through reasonable proportioning, so that the effects of the anti-inflammatory, the antibacterial peptide regulating and controlling, the antioxidant and the whitening effects are superior to the effects of the anti-inflammatory, the antibacterial peptide regulating and controlling, and the antioxidant and the whitening effects of the anti-oxidative and the whitening effects of the two components.
3. After a large number of natural medicinal plant extracts are screened through a cell experiment, the resveratrol is further determined to have the effects of inhibiting an inflammatory factor prostaglandin E2(PGE-2) and regulating and controlling the antibacterial peptide LL-37, and the resveratrol and the composition are reasonably compounded, so that the effects of resisting inflammation, regulating and controlling the antibacterial peptide, resisting oxidation and whitening can be further synergistically exerted.
Drawings
FIG. 1 shows the effect of examples 1-5, comparative examples 1-4 on PGE-2 secretion.
FIG. 2 shows the control of the antimicrobial peptide LL-37 in examples 1-5 and comparative examples 1-4.
FIG. 3 shows the effect of examples 6-10, comparative examples 5-8 on PGE-2 secretion.
FIG. 4 shows the control of the antimicrobial peptide LL-37 in examples 6-10 and comparative examples 5-8.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be easily understood by those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
Before the present embodiments are further described, it is to be understood that the scope of the invention is not limited to the particular embodiments described below; it is also to be understood that the terminology used in the examples is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention.
When numerical ranges are given in the examples, it is understood that both endpoints of each of the numerical ranges and any value therebetween can be selected unless the invention otherwise indicated. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It should be noted that the raw materials used in the present invention are all common commercial products, the sources thereof are not particularly limited, and the sources of some raw materials and the sources of the detection kit are shown in table 1.
TABLE 1
| Raw materials | Manufacturer of the product | Goods number |
| Schisandra chinensis | Beijing Tongrentang | Is free of |
| Saussurea involucrate cell culture | Ansaibo | Is free of |
| Resveratrol | Biotopped | 501-36-09 |
| PGE-2ELISA kit | Biotechwell | EH0424 |
| LL-37ELISA kit | CUSABIO | B04030098 |
| Stephania tetrandra extract | Jianmei biochemicals Co., Ltd, Zhejiang lake | 20210722 |
| L-tyrosine | BEIJING J&K SCIENTIFIC Ltd. | 185061 |
| Mushroom tyrosinase | JSENB | 9002-10-2 |
| Vitamin C | BEIJING J&K SCIENTIFIC Ltd. | 231406 |
Basic embodiment
An anti-inflammatory and/or whitening composition comprising the following raw materials: saussurea involucrate cell culture and schisandra chinensis;
preferably, the mass ratio of the saussurea involucrate cell culture and the schisandra chinensis in the composition is 1:3-4:1, and further preferably, the mass ratio is 1: 1.
The invention also discloses a preparation method of the anti-inflammatory composition, which comprises the following steps:
s1, mixing the saussurea involucrate cell culture, schisandra chinensis and 1, 3-butanediol, carrying out hot reflux extraction, carrying out fine filtration, and collecting filtrate to obtain an extracting solution;
s2, adding resveratrol into the extracting solution obtained in the step S1 to obtain the composition.
EXAMPLES 1-5 an anti-inflammatory composition and a process for its preparation
The anti-inflammatory composition materials and amounts described in examples 1-5 are shown in Table 2
TABLE 2
| Example 1 | Example 2 | Example 3 | Example 4 | Example 5 | |
| Saussurea involucrate cell culture/g | 2.5 | 5 | 6 | 7.5 | 8 |
| Schisandra fruit/g | 7.5 | 5 | 4 | 2.5 | 2 |
The preparation method comprises the following steps: the method comprises the following steps:
s1, weighing the cell culture of the saussurea involucrate and the schisandra chinensis, and comparing the weight of the saussurea involucrate and the schisandra chinensis according to the ratio of material to liquid: 30% of 1, 3-butanediol is added into the mixture at a ratio of 1: 50;
s2, carrying out thermal reflux at 100 ℃ for 2.5h, cooling, and then carrying out suction filtration by using a 0.45-micron filter plate;
s3, compounding with resveratrol of 0.2 percent to obtain the composition.
Comparative example 1
This comparative example differs from example 2 in that: the raw materials of the anti-inflammatory composition comprise 10g of saussurea involucrate cell culture, and not comprise schisandra chinensis.
Comparative example 2
This comparative example differs from example 2 in that: the raw materials of the anti-inflammatory composition comprise 10g of schisandra chinensis, and do not comprise saussurea involucrate cell cultures.
Comparative example 3
This comparative example differs from example 1 in that: the raw materials of the anti-inflammatory composition comprise 2g of saussurea involucrate cell culture and 8g of schisandra chinensis.
Comparative example 4
This comparative example differs from example 3 in that: the raw materials of the anti-inflammatory composition comprise 8.75g of saussurea involucrate cell culture and 1.25g of schisandra chinensis.
Evaluation of Effect
1. And (3) detecting the influence of the composition on PGE-2 secretion and the capability of regulating the antibacterial peptide LL-37.
(1) The detection method comprises the following steps:
s1, filtering the membranes of examples 1-5 and comparative examples 1-4 respectively, and preparing samples with concentrations of 5%, 2.5%, 1% and 0.5% respectively by using MEM culture medium;
s2, inoculation: according to 6X 105The cells were seeded at a seed/well density in 6-well plates and incubated overnight in an incubator (37 ℃, 5% CO 2);
s3, UVB irradiation: when the cell plating rate in the 6-well plate reaches 80% -90%, the culture solution is discarded, 1mL of PBS solution is added into each well, the cells are washed, and the washing is repeated for 2 times. Adding appropriate amount of PBS to cover the cells, and performing UVB radiation at a radiation dose of 100mJ/cm2；
S4, administration: setting a blank group, a UVB group and a sample group in an experiment, wherein the blank group is not irradiated by UVB and is inoculated into 2mL of culture medium; after UVB irradiation is carried out on the UVB group, 2mL of culture medium is inoculated; after UVB irradiation, the drug groups were inoculated into 2mL of sample media with different concentrations, at 37 ℃ and 5% CO2Continuously culturing for 24h in the incubator;
s5, collecting supernatant: after culturing for 24h, collecting cell culture supernatant in an EP tube, and freezing and storing in a refrigerator at-80 ℃;
s6, PGE-2 content detection: detecting the cell sample according to the PGE-2ELISA kit operating instruction;
s7, detecting the content of LL-37: detecting the cell sample according to the operating instruction of the LL-37ELISA kit;
s8, result statistical analysis: the test results were plotted using GraphPad Prism Program software, and the sample groups were compared to the negative control group, and by statistical analysis using SPSS, p <0.05 (x) indicated significant differences, and p <0.01 (x) indicated very significant differences.
(2) And (3) detection results: the results are shown in tables 3 to 4.
TABLE 3 results of assays for affecting PGE-2 secretion
TABLE 4 detection results of regulatory antimicrobial peptide LL-37
The antimicrobial peptides can be classified into various types according to their structures, of which Cathelicidin and Defensin are the main types, and the antimicrobial peptide LL-37 is the only antimicrobial peptide present in human skin in the Cathelicidin family. It not only has bactericidal effect, but also plays a key role in the release of inflammatory factors in immune regulation and the occurrence of inflammatory diseases. Research has shown that LL-37 can resist the increase of NF-kB protein expression and transcription activity caused by UVB radiation HaCaT cells, and inhibit the release of proinflammatory cytokines PGE-2 and the like, thereby inhibiting inflammatory reaction. UVB radiation causes cells to generate inflammatory reaction, the expression of the antibacterial peptide LL-37 is increased, the release of inflammatory factors PGE-2 and the like is inhibited, so that the inflammatory reaction is inhibited, after the inflammatory reaction is reduced, the expression of the antibacterial peptide LL-37 is reduced, and the regulation is adjusted to be in a balanced state, so that the regulation of LL-37 has important influence on the inhibition of the inflammatory reaction.
2. And detecting the antioxidant effect of the composition.
(1) The detection method comprises the following steps:
s1, sample preparation: weighing 20mg of DPPH, adding absolute ethyl alcohol to dissolve, and fixing the volume in a 250mL volumetric flask, wherein the concentration of DPPH solution is 2 multiplied by 10-4mol/L, keeping away from light at 0-4 ℃, using the product when preparing the product, and being effective within 4 h; vitamin C is selected as a positive control, and the concentration of the vitamin is 1 mg/mL; examples 1-5 and p with anhydrous ethanolThe samples to be tested were prepared at 6 concentrations, 100%, 50%, 10%, 5%, 1% and 0.5% for the ratios 1-4.
S2, mixing 1mL of solution to be detected with 1mL of DPPH solution (tube A); 1mL of absolute ethanol was mixed with 1mL of DPPH solution (tube B); 1mL of absolute ethanol and 1mL of solution to be tested were mixed (tube C) (shown in Table 5); after 30min of reaction, the absorbance of A, B, C tubes was measured at 517nm using a microplate reader (infinite M200 PRO, TECAN).
TABLE 5
| Numbering | DPPH solution | Anhydrous ethanol | Liquid to be tested | Total volume |
| A | 1.0mL | \ | 1.0mL | 2.0mL |
| B | 1.0mL | 1.0mL | \ | 2.0mL |
| C | \ | 1.0mL | 1.0mL | 2.0mL |
S3, data processing: the clearance (%) ([ (B + C) -a ]/B).
(2) And (3) detection results: the results are shown in Table 6.
TABLE 6 measurement results of DPPH clearance
3. And detecting the whitening effect of the composition.
(1) The detection method comprises the following steps:
s1, sample preparation: weighing 45.30mg of L-tyrosine, dissolving the L-tyrosine in 50mL of PBS buffer solution to prepare 5mM L-tyrosine solution, and storing the solution at 4 ℃ for later use; completely dissolving 25kU of mushroom tyrosinase powder in 2mL of precooled sterile PBS solution, subpackaging 50 mu L of each tube, storing at-20 ℃, taking 50 mu L of mushroom tyrosinase solution in one tube when in use, adding 2.45mL of PBS buffer solution, diluting to 2.5mL for use, storing at 4 ℃ for later use, and using up within 24 h; vitamin C is selected as a positive control, and the concentration of the vitamin C is 1 mg/mL; examples 1 to 5 and comparative examples 1 to 4 were prepared with PBS solutions at 5 concentrations of 0.5%, 1%, 5%, 10% and 100%, respectively, as samples to be tested.
S2, preheating the mushroom tyrosinase, the prepared sample to be tested, PBS and L-tyrosine solution in a shaking table at 37 ℃ for 10 min; adding 20 μ L of sample solution to be tested and PBS solvent into 96-well culture plate, adding 160 μ L of L-tyrosine (0.5mM), mixing, and keeping temperature in 37 deg.C incubator (KYC-100B, Shanghai Fuma experiment equipment Co., Ltd.); adding 20 μ L of 250U/mL mushroom tyrosinase solution preheated at 37 deg.C (final concentration is 25U/mL), mixing (reagent formula shown in Table 7), and immediately measuring absorbance values A0 and B0 at 490nm with enzyme labeling instrument (infinite M200 PRO, TECAN); after the determination, the mixture is placed in a constant temperature incubator at 37 ℃ for reaction for 30min, and absorbance values A30 and B30 at 490nm are determined by an enzyme-labeling instrument.
TABLE 7
| Reagent | A (sample group) | B (solvent group) |
| L-tyrosine solution (μ L) | 160 | 160 |
| Sample to be tested or PBS solvent (μ L) | 20 | 20 |
| Mushroom tyrosinase solution (μ L) | 20 | 20 |
S3, data processing:
(3) and (3) detection results: the results are shown in Table 8.
TABLE 8 tyrosinase inhibition assay results
The detection results show that the example 1-5 with the mass ratio of the saussurea involucrate cell culture to the schisandra chinensis within the protection range of the invention can obviously inhibit the secretion of PGE-2, and simultaneously regulate and control the content of the antibacterial peptide LL-37 to restore the antibacterial peptide LL-37 to a balanced state, and the anti-inflammatory, antibacterial peptide regulation and control capacity, and the antioxidant and whitening capacity of the saussurea involucrate cell culture composition and the schisandra chinensis composition in the comparative example 1-2 are superior to those of the saussurea involucrate cell culture composition and the schisandra chinensis composition in the comparative example 1-2. The test results of comparative examples 1-5 and comparative examples 3-4 show that the anti-inflammatory composition, in which the mass ratio of the saussurea involucrate cell culture to the schisandra chinensis is not within the protection range of the present invention, can not exert the synergistic effect.
EXAMPLES 6 to 10 an anti-inflammatory composition and a process for the preparation thereof
The anti-inflammatory composition materials and amounts described in examples 6-10 are shown in Table 9
TABLE 9
| Example 6 | Example 7 | Example 8 | Example 9 | Example 10 | |
| Saussurea involucrate cell culture/g | 5 | 5 | 5 | 5 | 5 |
| Schisandra fruit/g | 5 | 5 | 5 | 5 | 5 |
| Resveratrol/% | 0.1 | 0.15 | 0.2 | 0.25 | 0.3 |
The preparation method comprises the following steps: the method comprises the following steps:
s1, weighing the cell culture of the saussurea involucrate and the schisandra chinensis, and comparing the weight of the saussurea involucrate and the schisandra chinensis according to the ratio of material to liquid: 30% of 1, 3-butanediol is added into the mixture at a ratio of 1: 50;
s2, carrying out thermal reflux at 100 ℃ for 2.5h, cooling, and then carrying out suction filtration by using a 0.45-micron filter plate;
s3, compounding with resveratrol of 0.2 percent to obtain the composition.
Comparative example 5
This comparative example differs from example 8 in that: the raw materials of the anti-inflammatory composition comprise 10g of saussurea involucrate cell culture, and not comprise schisandra chinensis.
Comparative example 6
This comparative example differs from example 8 in that: the raw materials of the anti-inflammatory composition comprise 10g of schisandra chinensis, and do not comprise saussurea involucrate cell cultures.
Comparative example 7
This comparative example differs from example 8 in that: the raw material of the anti-inflammatory composition comprises 5g of peony, and does not comprise saussurea involucrate cell culture.
Comparative example 8
This comparative example differs from example 8 in that: the raw materials of the anti-inflammatory composition comprise 5g of scutellaria baicalensis, and schisandra chinensis is not included.
Evaluation of Effect
1. And (3) detecting the influence of the composition on PGE-2 secretion and the capability of regulating the antibacterial peptide LL-37.
(1) Detection method
Consistent with the detection method described above.
(2) And (3) detection results: the results are shown in tables 10 to 11
TABLE 10 test results for effects on PGE-2 secretion
TABLE 11 test results for regulatory antimicrobial peptide LL-37
2. And detecting the antioxidant effect of the composition.
(1) Detection method
Consistent with the detection method described above.
(2) And (3) detection results: the results are shown in Table 12.
TABLE 12 measurement results of DPPH clearance
3. And detecting the whitening effect of the composition.
(1) The detection method comprises the following steps:
in accordance with the above-mentioned detection method
(2) And (3) detection results: the results are shown in Table 13.
TABLE 13 tyrosinase inhibition assay results
The detection results show that the mass ratio of the saussurea involucrate cell culture, the schisandra chinensis and the resveratrol in the protection range of the invention can obviously inhibit the secretion of PGE-2, and simultaneously regulate and control the content of the antibacterial peptide LL-37 to restore the antibacterial peptide to a balanced state, and the effects of resisting inflammation, regulating and controlling the antibacterial peptide and the effects of resisting oxidation and whitening are superior to those of a composition compounded by the saussurea involucrate cell culture and the schisandra chinensis, and the detection results of the comparative examples 6-10 and the example 2 prove that the three natural medicinal plant raw materials are compounded according to a certain proportion, so that the anti-inflammation, the capacity of regulating and controlling the antibacterial peptide and the capacities of resisting oxidation and whitening can be synergistically enhanced. The test results of the comparative example 8 and the comparative examples 7 to 8 show that the anti-inflammatory, antioxidant and whitening effects are remarkably reduced even if one of the raw materials is replaced by other natural medicinal plant raw materials with the same weight part and anti-inflammatory and antibacterial effects, and the test results of the comparative examples 6 to 10 and the comparative examples 5 to 6 show that the saussurea involucrate cell culture, the schisandra chinensis and the resveratrol with specific mass ratios can play a synergistic effect.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.
Claims (10)
1. An anti-inflammatory composition, comprising the following raw materials: herba Saussureae Involueratae cell culture and fructus Schisandrae chinensis.
2. The composition as claimed in claim 1, wherein the mass ratio of the saussurea involucrate cell culture to the schisandra chinensis extract is 1:3-4: 1.
3. The composition of claim 1, wherein said composition further comprises resveratrol.
4. The composition of claim 3, wherein the resveratrol is present in an amount of 0.1% to 0.3% by weight.
5. A process for the preparation of a composition according to any one of claims 1 to 4, characterized in that it comprises the following steps:
s1, mixing the saussurea involucrate cell culture, schisandra chinensis and 1, 3-butanediol, carrying out hot reflux extraction, carrying out fine filtration, and collecting filtrate to obtain an extracting solution;
s2, adding resveratrol into the extracting solution obtained in the step S1 to obtain the composition.
6. Use of a composition according to any one of claims 1 to 4 for the preparation of an anti-inflammatory, whitening, antioxidant and/or antimicrobial peptide modulating product.
7. An anti-inflammatory, whitening, antioxidant and/or antimicrobial peptide modulating product comprising the composition of any of claims 1-4.
8. The product of claim 7, wherein said product is a cosmetic product.
9. The product of claim 8, wherein the cosmetic comprises a cream, a lotion, a serum, a makeup cream, a barrier cream, a BB cream, a foundation, a shampoo, a conditioner, or a toner for skin care.
10. Use of the composition of any one of claims 1-4 for anti-inflammatory, whitening, antioxidant and/or modulating antimicrobial peptides.
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