CN113817799B - Method for identifying and evaluating resistance level of pleurotus geesteranus to physalospora naissacensis induced maculopathy - Google Patents
Method for identifying and evaluating resistance level of pleurotus geesteranus to physalospora naissacensis induced maculopathy Download PDFInfo
- Publication number
- CN113817799B CN113817799B CN202111315412.0A CN202111315412A CN113817799B CN 113817799 B CN113817799 B CN 113817799B CN 202111315412 A CN202111315412 A CN 202111315412A CN 113817799 B CN113817799 B CN 113817799B
- Authority
- CN
- China
- Prior art keywords
- pleurotus geesteranus
- disease
- pathogenic bacteria
- culture
- yellow spot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000222350 Pleurotus Species 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 17
- 208000035719 Maculopathy Diseases 0.000 title claims description 9
- 208000002780 macular degeneration Diseases 0.000 title claims description 9
- 241001115351 Physalospora Species 0.000 title description 2
- 244000052616 bacterial pathogen Species 0.000 claims abstract description 40
- 201000010099 disease Diseases 0.000 claims abstract description 40
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 40
- 241000233866 Fungi Species 0.000 claims abstract description 22
- 239000000725 suspension Substances 0.000 claims abstract description 16
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 7
- 238000012258 culturing Methods 0.000 claims abstract description 5
- 230000004936 stimulating effect Effects 0.000 claims abstract description 4
- 238000011081 inoculation Methods 0.000 claims description 20
- 241000894006 Bacteria Species 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 6
- 230000001717 pathogenic effect Effects 0.000 claims description 5
- 208000035240 Disease Resistance Diseases 0.000 claims description 4
- 238000011156 evaluation Methods 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 238000004364 calculation method Methods 0.000 claims description 3
- 208000038015 macular disease Diseases 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000007865 diluting Methods 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 claims description 2
- 241001052560 Thallis Species 0.000 claims 2
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract 2
- 241001018513 Naringi Species 0.000 abstract 1
- 241000343419 Nernstia Species 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 4
- 241000588653 Neisseria Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000043250 Cedecea neteri Species 0.000 description 1
- 208000018380 Chemical injury Diseases 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 241000392443 Pleurotus citrinopileatus Species 0.000 description 1
- 241001138370 Pleurotus pulmonarius Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for identifying and evaluating resistance level of pleurotus geesteranus to northern leaf blight induced by western Naringi, which comprises the following steps: inoculating pleurotus geesteranus strains on the cultivation material belt, culturing mycelia for 80 days, and stimulating mushroom sticks to grow mushrooms by temperature difference after the mycelia are physiologically mature; taking a western nernstia bacterial strain, and performing shake culture to obtain a pathogenic bacteria suspension; selecting a neat and healthy fungus stick with a plurality of sporocarps, and inoculating when the size of a pileus of the sporocarps is 0.5-1.0 cm; placing the mixture in a sealed fresh-keeping box, and then transferring the mixture to a proper temperature and humidity condition for culture; after culturing for 2 days, taking out the fungus sticks, investigating the disease incidence of pleurotus geesteranus yellow spot, recording the number and disease incidence grade of diseased fruiting bodies of the fungus sticks, calculating disease indexes, and judging the resistance level of pleurotus geesteranus yellow spot. The method can accurately identify the resistance level of the yellow spot of the pleurotus geesteranus with different varieties, has reliable results, and has important significance for promoting the breeding work of the disease-resistant pleurotus geesteranus yellow spot.
Description
Technical Field
The invention relates to the field of crop disease resistance evaluation, in particular to a method for identifying and evaluating the resistance level of pleurotus geesteranus to sigatoka induced by west neisseria naissance.
Background
Pleurotus geesteranus, the famous pleurotus citrinopileatus, is an edible fungus with rich nutrition and delicious taste. The protein content of the vegetable is close to that of meat, is 3-6 times higher than that of common vegetables, and is a delicious and cheap top-grade dish on a dining table of people for a long time.
The yellow spot is the most important disease in pleurotus geesteranus production. The early test results show that the pathogenic bacteria of the maculopathy is West Neisseria. As the environment humidity is high, the temperature is high, the ventilation is not smooth during the fruiting period of the pleurotus geesteranus, and the yellow spot is easy to occur. If the mushroom is not treated in time, the mushroom is easy to spread and explode, which causes that the whole mushroom shed is dead in production and dead in harvest, and causes great economic loss.
Maculopathy occurs in the fruiting body of Pleurotus geesteranus, and only in the fruiting stage. The fruiting period of edible mushrooms such as pleurotus geesteranus is short, if the medicament is applied in the fruiting period, medicament residues are easily caused to cause food poisoning, further serious influence is caused on product circulation and consumption, and sometimes serious chemical injury is caused to the edible mushrooms, so that the medicament is strictly forbidden to be applied in the fruiting period of the pleurotus geesteranus. In addition, the yellow spot is generated rapidly, so that the control of the yellow spot of the pleurotus geesteranus is very difficult.
In view of this, the breeding and popularization of disease-resistant varieties is undoubtedly the most economic, effective and environment-friendly method for preventing and controlling the yellow spot of pleurotus geesteranus. The establishment of a simple and effective resistance identification technical system is the premise and key for developing the research, but no report of an identification evaluation standard method for pleurotus geesteranus yellow spot exists at present, so that the development of the resistance identification method for pleurotus geesteranus yellow spot is of great significance for promoting the research on pleurotus geesteranus yellow spot.
Disclosure of Invention
The invention aims to provide a method for identifying and evaluating the level of resistance of pleurotus geesteranus to yellow spot disease induced by west neisseria nethii, so as to solve the problems in the prior art and lay a technical foundation for breeding and screening of anti-yellow spot pleurotus geesteranus varieties, identification of pathogenic bacteria pathogenic strength and weakness and prevention and control of pleurotus geesteranus yellow spot disease.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a method for identifying and evaluating the resistance level of pleurotus geesteranus yellow spot by utilizing fruiting body inoculation, which comprises the following steps:
(1) preparation of Pleurotus geesteranus stick
Inoculating pleurotus geesteranus strains into the cultivation material bag, culturing mycelia for 8 days, and stimulating mushroom sticks to grow mushrooms by temperature difference after the mycelia are physiologically mature;
(2) preparation of a suspension of pathogenic bacteria
Taking a bacterial strain of the pathogenic bacteria of the maculopathy, placing the bacterial strain in a liquid NB culture medium, and performing shake culture for 2 days to obtain a pathogenic bacteria suspension;
(3) pleurotus geesteranus fruiting and pathogenic bacteria inoculation
Selecting regular and healthy fungus sticks with more sporocarps, and inoculating when the diameter of the pileus of the sporocarps is 0.5-1.0 cm;
when the pathogenic bacteria are inoculated, a sterile brush is adopted to dip the pathogenic bacteria suspension liquid, and the pathogenic bacteria suspension liquid is uniformly smeared on the pleurotus geesteranus sporocarp;
after the pathogenic bacteria are inoculated, the bacteria stick is placed in a closed fresh-keeping box for culture;
(4) evaluation of disease resistance level
After the culture is finished, the fungus sticks are taken out, the disease occurrence condition of the pleurotus geesteranus yellow spot is investigated, the number and disease level of the diseased fruiting bodies of each fungus stick are recorded, the disease index is calculated, and the resistance level of the pleurotus geesteranus yellow spot is judged according to the disease index.
Further, in the step (2), the shaking culture condition is that the culture is carried out at the temperature of 28 ℃ and the rpm/min of 180 for 48 h.
Further, in the step (2), after the shake culture is finished, the bacterial liquid is centrifuged, the precipitate is collected to obtain the thallus, and the thallus is diluted by sterile water to obtain the pathogenic bacteria suspension.
Further, the pathogenic strain of the macular disease is West Neelicidium.
Further, the centrifugation is 5000rpm/min for 2 min.
Further, the number of pathogenic bacteria in the pathogenic bacteria suspension is 1x108One per ml.
Further, in the step (3), the number of the fungus sticks inoculated with pathogenic bacteria of each variety is not less than 10, and the number of the fruit bodies on each fungus stick is not less than 20.
Further, in the step (3), during cultivation, the fungus sticks are placed in the sealed preservation box and are 2-8cm away from the box wall, and the fruiting surface is 10-20cm away from the box wall.
Further, in the step (3), the culture conditions are that the temperature is 25-30 ℃, the humidity is 85-100%, and the culture is carried out for 48 h.
The invention discloses the following technical effects:
the selection of the inoculation time is very critical, the diameter of the pileus of the fruiting body is selected to be 0.5-1.0cm for inoculation, and in the actual production, in order to promote the growth of the stipe of the fruiting body, the environment of the mushroom shed at the stage requires high oxidation concentration, but the disease resistance of young mushrooms is weak;
the inoculation mode is simple, no puncture is needed, and the requirements of natural morbidity in production are met;
the environmental conditions (temperature, humidity and closed environment) during the culture after inoculation are very important, and the conditions for normal fruiting of pleurotus geesteranus and suitable disease attack are created.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is the morphology of a pathogen of maculopathy on NA medium;
FIG. 2 shows the morphology of pathogenic bacteria of maculopathy under electron microscope;
FIG. 3 shows the natural onset of yellow spot disease of Pleurotus geesteranus;
FIG. 4 is a Pleurotus geesteranus stick with fruiting body of suitable size for inoculating pathogenic bacteria;
FIG. 5 shows the disease of Pleurotus geesteranus of different species after inoculation of pathogenic bacteria of yellow spot;
FIG. 6 shows the fruiting body of Pleurotus geesteranus after inoculation with overlarge pileus;
FIG. 7 shows the fruiting body of Pleurotus geesteranus after the cap is too small to be inoculated.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
Example 1
1. Preparation of pleurotus geesteranus stick
The method comprises the steps of preparing raw materials by adopting a conventional formula of pleurotus geesteranus, bagging, carrying out thorough sterilization, cooling, and inoculating pleurotus geesteranus of different varieties (strains) according to aseptic operation. After inoculation, the culture medium is placed into a culture room for hypha culture. And (5) stimulating the fungus sticks to produce mushrooms when the hypha reaches physiological maturity.
2. Pathogenic bacteria preparation
The pathogenic bacterium of Yellow spot is West Neeli (Liu Z L, Zhou S, Zhang W, et al. first Report of Cedecea neteri consuming Yellow Rot Disease in Pleurotus pulmonarius in China [ J ] Plant Disease,2020.) which is isolated from the fruiting body of Pleurotus geesteranus infected with Yellow spot in Guangxi and deposited in the institute of microbiology of Guangxi institute of agricultural sciences, the form of the pathogenic bacterium on NA medium is shown in FIG. 1, and the form under electron microscope is shown in FIG. 2. The pathogenic bacteria cause the natural attack of Pleurotus geesteranus as shown in FIG. 3.
Strain activation: placing the macular disease pathogenic bacteria strain in a refrigerator at-80 deg.C for long-term storage, taking out part of the strain stored at-80 deg.C before inoculation, placing in a liquid NB culture medium, performing shake culture at 28 deg.C and 180rpm/min for 48 hr to obtain bacterial liquid, centrifuging the bacterial liquid (5000rpm/min, 2min), collecting precipitate to obtain thallus, diluting with sterile water to 1x108And (4) obtaining an inoculation bacterial suspension for later use.
3. Pleurotus geesteranus fruiting and pathogenic bacteria inoculation method
And selecting bacteria sticks with multiple fruiting bodies, tidiness and health for carrying out subsequent pathogen inoculation tests, wherein the number of the bacteria sticks inoculated to each variety is not less than 10. When the size of pileus of fruiting body is 0.5-1.0cm, inoculation (figure 4) is too big to cause disease (figure 6), and when inoculation is too small, fruiting body is easy to wither and die directly and cannot grow up (figure 7). And (3) dipping the pathogenic bacteria suspension by using a sterile brush, uniformly coating the pathogenic bacteria suspension on pleurotus geesteranus fruiting bodies for inoculation, and coating 5ml of bacterial liquid on each bacterial stick. After inoculation, the fungus sticks are moved to a sealed fresh-keeping box to be horizontally placed in a layered mode (the fungus sticks are preferably 3-8cm away from the box wall in the box, the fruiting side is preferably 10-20cm away from the box wall, fruiting is influenced if the distance is too small, sporophores cannot be completely unfolded, and if the distance is too large, high CO in the fresh-keeping box cannot be kept2Concentration, resulting in difficult development of fruiting body). The temperature of the preservation box is kept at 25-30 ℃, and the humidity is kept at 85-100%. Taking out the fungus stick after 48h, and pattingAccording to the figure (figure 5), the number of the fruiting bodies of each mushroom stick is calculated (the disease is calculated whether the pileus or the stipe exists or only symptoms exist), grading is carried out according to the disease incidence, and the disease index is calculated.
4. Disease grade grading standard
And calculating the total number of the diseased sporocarps and the sporocarps on each bacteria stick, dividing the number of the diseased sporocarps and the total number of the sporocarps to obtain the morbidity of a single bacteria stick, and grading the bacteria sticks respectively according to the morbidity. The classification criteria are as follows:
5. calculation of disease indexes of different varieties
Disease index (n)0*0+n1*1+n3*3+n5*5+n7*7)*100/(N*7)。
N represents the total number of bacteria sticks, N represents the number of bacteria sticks per disease grade, and 0, 1, 3, 5, and 7 represent disease grades.
6. The resistance of the pleurotus geesteranus yellow spot of different varieties is classified according to the disease index, and the standard is as follows:
7. statistics of resistance levels of different varieties of pleurotus geesteranus yellow spot
As shown in table 1.
TABLE 1 statistics of the resistance level to yellow spot of various Pleurotus geesteranus species
As can be seen from the results in the table above, pathogenic bacteria of yellow spot can infect the Pleurotus geesteranus materials of the above varieties and cause plant diseases. The results confirm that Xiuzhen 12 is indeed a high-resistant variety against maculopathy, consistent with the actual planting experience. Therefore, the method for identifying and evaluating the pleurotus geesteranus yellow spot resistance level by utilizing the sporocarp inoculation can accurately identify the yellow spot resistance level of different varieties of pleurotus geesteranus, has reliable results, and has important significance for promoting the breeding work of the disease-resistant pleurotus geesteranus yellow spot varieties.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (1)
1. A method for identifying and evaluating the resistance level of pleurotus geesteranus yellow spot by utilizing fruiting body inoculation is characterized by comprising the following steps:
(1) preparation of Pleurotus geesteranus stick
Inoculating pleurotus geesteranus strains on the cultivation material belt, culturing mycelia for 80 days, and stimulating mushroom sticks to grow mushrooms by temperature difference after the mycelia are physiologically mature;
(2) preparation of a suspension of pathogenic bacteria
Taking a bacterial strain of the pathogenic bacteria of the maculopathy, placing the bacterial strain in a liquid NB culture medium, and performing shake culture for 2 days to obtain a pathogenic bacteria suspension;
(3) pleurotus geesteranus fruiting and pathogenic bacteria inoculation
Selecting regular and healthy fungus sticks with more sporocarps, and inoculating when the size of a pileus is 0.5-1.0 cm;
when the pathogenic bacteria are inoculated, a sterile brush is adopted to dip the pathogenic bacteria suspension liquid, and the pathogenic bacteria suspension liquid is uniformly smeared on the pleurotus geesteranus sporocarp;
after inoculating pathogenic bacteria, placing the fungus sticks in a closed preservation box for culturing; keeping high CO in the fresh-keeping box in the culture process2Concentration;
(4) evaluation of disease resistance level
After the culture is finished, taking out the fungus sticks, investigating the disease condition of the pleurotus geesteranus yellow spot, recording the number and disease grade of the diseased fruiting bodies of each fungus stick, calculating disease index, and judging the resistance level of the pleurotus geesteranus yellow spot;
in the step (4), the incidence rate of a single bacterium stick is obtained according to the fruiting body with disease on each bacterium stick and the total quantity of the fruiting bodies, and the calculation method comprises the following steps:
incidence = number of fruiting bodies/total number of fruiting bodies diseased on each stick;
the fungus sticks are respectively graded according to the morbidity, and the grading standard is as follows:
the calculation formula of the disease index is as follows:
disease index = (n)0*0+n1*1+n3*3+n5*5+n7*7）*100/(N*7)；
Wherein N represents the total number of bacteria sticks, N represents the number of bacteria sticks of each disease grade, and 0, 1, 3, 5 and 7 represent disease grades;
classifying the resistance levels of the yellow spot diseases of different pleurotus geesteranus varieties according to the disease indexes, wherein the standard for judging the resistance levels of the yellow spot diseases of the pleurotus geesteranus varieties is as follows:
the macular disease pathogenic bacterial strain is western Naegeli;
in the step (2), after the shake culture is finished, centrifuging a bacterium solution, collecting precipitates to obtain thalli, and diluting the thalli with sterile water to obtain the pathogenic bacteria suspension;
in the step (2), the shake culture condition is culture at 28 ℃ and 180rpm/min for 48 h;
the centrifugation is carried out for 2min at 5000 rpm/min;
the number of pathogenic bacteria in the pathogenic bacteria suspension is 1x108Each strain rod is inoculated with 5ml of pathogenic bacteria liquid;
in the step (3), the number of the fungus sticks inoculated with pathogenic bacteria of each variety is not less than 10, and the number of the fruit bodies on each fungus stick is not less than 20;
in the step (3), during cultivation, the fungus sticks are placed in the sealed preservation box and are 2-8cm away from the box wall, and the fruiting surface is 10-20cm away from the box wall;
in the step (3), the culture conditions are that the temperature is 25-30 ℃, the humidity is 85-100%, and the culture is carried out for 48 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111315412.0A CN113817799B (en) | 2021-11-08 | 2021-11-08 | Method for identifying and evaluating resistance level of pleurotus geesteranus to physalospora naissacensis induced maculopathy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111315412.0A CN113817799B (en) | 2021-11-08 | 2021-11-08 | Method for identifying and evaluating resistance level of pleurotus geesteranus to physalospora naissacensis induced maculopathy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113817799A CN113817799A (en) | 2021-12-21 |
| CN113817799B true CN113817799B (en) | 2022-05-17 |
Family
ID=78917864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111315412.0A Active CN113817799B (en) | 2021-11-08 | 2021-11-08 | Method for identifying and evaluating resistance level of pleurotus geesteranus to physalospora naissacensis induced maculopathy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113817799B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113881792B (en) * | 2021-11-23 | 2022-05-24 | 广西壮族自治区农业科学院 | PCR detection primer and kit for West nerviliae and application of PCR detection primer and kit |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416224A (en) * | 2013-08-08 | 2013-12-04 | 刘万顺 | Method for preparing pleurotus geesteranus compost |
| CN103918475A (en) * | 2014-03-26 | 2014-07-16 | 上海惠芬果蔬专业合作社 | Pleurotus geesteranus potted culturing method and culture medium for culturing pleurotus geesteranus |
| CN104012303A (en) * | 2014-06-30 | 2014-09-03 | 厦门慧翔食用菌种植有限公司 | Cultivating method of pleurotus geesteranus edible mushroom |
| CN104303833A (en) * | 2014-09-28 | 2015-01-28 | 铜陵市香江食用菌种植有限责任公司 | Method for cultivating Taiwan pleurotus geesteranus in north of Anhui province |
| CN104521561A (en) * | 2014-12-24 | 2015-04-22 | 广东省农业科学院蚕业与农产品加工研究所 | Cultivation method for secondary pleurotus geesteranus fruiting |
| CN105532264A (en) * | 2016-01-15 | 2016-05-04 | 玉林市微生物研究所 | Out-of-season efficient pleurotus geesteranus cultivation method |
-
2021
- 2021-11-08 CN CN202111315412.0A patent/CN113817799B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416224A (en) * | 2013-08-08 | 2013-12-04 | 刘万顺 | Method for preparing pleurotus geesteranus compost |
| CN103918475A (en) * | 2014-03-26 | 2014-07-16 | 上海惠芬果蔬专业合作社 | Pleurotus geesteranus potted culturing method and culture medium for culturing pleurotus geesteranus |
| CN104012303A (en) * | 2014-06-30 | 2014-09-03 | 厦门慧翔食用菌种植有限公司 | Cultivating method of pleurotus geesteranus edible mushroom |
| CN104303833A (en) * | 2014-09-28 | 2015-01-28 | 铜陵市香江食用菌种植有限责任公司 | Method for cultivating Taiwan pleurotus geesteranus in north of Anhui province |
| CN104521561A (en) * | 2014-12-24 | 2015-04-22 | 广东省农业科学院蚕业与农产品加工研究所 | Cultivation method for secondary pleurotus geesteranus fruiting |
| CN105532264A (en) * | 2016-01-15 | 2016-05-04 | 玉林市微生物研究所 | Out-of-season efficient pleurotus geesteranus cultivation method |
Non-Patent Citations (8)
| Title |
|---|
| First Report of Cedecea neteri Causing Yellow Rot Disease in Pleurotus pulmonarius in China;Zengliang Liu et al;《Plant Disease》;20201103;摘要，图S1 * |
| 刘川等.平菇细菌性黄斑病发病因素分析.《食用菌学报》.2013,(第01期), * |
| 平菇细菌性黄斑病发病因素分析;刘川等;《食用菌学报》;20130315(第01期);第101-104页 摘要，第1-3节 * |
| 平菇锈斑病发病症状识别及病原菌的形态特征鉴定;陈雪凤等;《园艺与种苗》;20170815(第08期);第12-14、28页 * |
| 抗黄斑病深色平菇品种筛选试验;决超等;《北京农业》;20131025(第30期);第127-128页 * |
| 浙江秀珍菇黄菇病病原菌的分离与鉴定;袁卫东等;《浙江农业学报》;20181129(第11期);第1893-1898页 * |
| 秀珍菇主要病虫害危害特征及防治对策;陈英;《农业灾害研究》;20121215;第13-16页 * |
| 秀珍菇黄菇病的调查与防治建议;汪彩云等;《蔬菜》;20100115(第01期);第26-27页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113817799A (en) | 2021-12-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Awa et al. | First report of fruit anthracnose in mango caused by Colletotrichum gloeosporioides in Southwestern Nigeria | |
| JP5664984B2 (en) | New strain Shiitake GNA01 | |
| CN110699275A (en) | Bacterial strain for antagonizing botrytis cinerea pathogenic bacteria after fruit and vegetable picking and application thereof | |
| CN107227263B (en) | White flammulina velutipes JK01 strain and application thereof | |
| CN101647370A (en) | Standardized high-yield culture technique based on biological characteristics of asafetida mushroom | |
| Tian et al. | Effect of temperature during conidial formation of Monilinia laxa on conidial size, germination and infection of stored nectarines | |
| CN108611287A (en) | The Pichia membranaefaciens of one plant of control peach fruit postharvest disease | |
| CN108522264A (en) | A kind of crossbreeding technique of high temperature modification mushroom L16 | |
| CN113817799B (en) | Method for identifying and evaluating resistance level of pleurotus geesteranus to physalospora naissacensis induced maculopathy | |
| KR101305243B1 (en) | A novel strain pleurotus nebrodensis and method of producing it | |
| KR101312059B1 (en) | A novel strain pleurotus nebrodensis | |
| EA035884B1 (en) | New hybrid agaricus bisporus mushroom strains | |
| CN115074259B (en) | Alkali-resistant lentinus edodes strain golden fragrance 194 and application thereof | |
| CN101748073B (en) | Method for separating and preserving Cordyceps militaris spawn | |
| CN101343614B (en) | Fungi Imperficti strain and uses thereof | |
| CN102668881B (en) | Hericium coralloides | |
| Agu et al. | Isolation, identification and pathogenicity of fungi associated with cocoyam (Colocasia esculenta) spoilage | |
| CN107488702A (en) | A kind of new identification sunflower method horizontal to resistance to sclerotinia sclerotiorum | |
| Xiao | Postharvest fruit rots in d'Anjou pears caused by Botrytis cinerea, Potebniamyces pyri, and Sphaeropsis pyriputrescens | |
| Lee et al. | Varietal characteristics of new white button mushroom'Seolwon'in Agaricus bisporus | |
| CN101824461B (en) | Method for quickly detecting fruiting performance of pleurotus nebrodensis | |
| CN102293107B (en) | Method for rapidly identifying black spot resistance of radish germplasms | |
| CN115093976B (en) | White beech mushroom ZHSjg-byg and cultivation method thereof | |
| Yusuf et al. | Fungi associated with the storage rot of yam (Discorea rotundata Poir) in Yola, Adamawa State | |
| CN109526554A (en) | A kind of implantation methods of edible mushroom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |