CN113604565A - 一种基于ngs方法检测人类brca1和brca2基因突变的引物组合物及其试剂 - Google Patents

一种基于ngs方法检测人类brca1和brca2基因突变的引物组合物及其试剂 Download PDF

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CN113604565A
CN113604565A CN202110723978.0A CN202110723978A CN113604565A CN 113604565 A CN113604565 A CN 113604565A CN 202110723978 A CN202110723978 A CN 202110723978A CN 113604565 A CN113604565 A CN 113604565A
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宋钢
王朝晖
王强
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Abstract

本发明公开了一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，包括：263对的第一组引物，20对的第二组引物；每对第一组引物或第二组引物均包括正向引物序列与反向引物序列，且所述第一组引物的正向引物序列与反向引物序列均包括可特异性扩增BRCA1/BRCA2基因外显子、内含子或UTR区域的序列，所述第二组引物的正向引物序列与反向引物序列均包括可特异性扩增人类基因组特定参考序列。根据本发明，可有效地检测BRCA1/BRCA2基因的致病点突变、短缺失/***以及拷贝数变异，既可以检测基因组DNA样本，又可以检测肿瘤FFPE样本，可满足对于卵巢癌、乳腺癌的遗传风险评估，以及PARPi抑制剂用药指导，预后评估等目的，整体流程简单高效，具有良好的前景。

Description

一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组 合物及其试剂
技术领域
本发明涉及基因组合物的技术领域，特别涉及一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物及其试剂。
背景技术
根据2018年全球癌症统计报告显示，全球女性乳腺癌新发病例占总体的11.6％，与肺癌并列第一。乳腺癌是女性最常见的癌症(发病率为24.2％)，乳腺癌死亡率为15.0％，发病率和死亡率均居于女性发病和死亡的首位。卵巢癌发病率占比3.4％，死亡率占比为4.4％，其发病率和死亡率位于女性发病率和死亡率的第八位。
研究表明，约15％的乳腺癌和卵巢癌患者携带胚系(germline，即来自父母生殖细胞先天遗传)的BRCA1和/或BRCA2基因突变。携带此类胚系突变的女性在25至30岁时就有可能患上乳腺癌或者卵巢癌，而且随着年龄的增长其发病风险逐渐增加，最高竟可达60％至80％。
针对BRCA1和BRCA2基因的功能，目前已经开发了靶向治疗药物—— PARP抑制剂(PARP inhibitor,PARPi)。PARP是一种单链DNA修复酶，与BRCA1/2 基因参与的双链DNA修复机制共同组成细胞主要的DNA修复方式。当BRCA 基因突变后，双链DNA修复机制受阻。这时使用PARPi，能进一步阻滞单链 DNA修复机制，促进癌细胞的DNA损伤得不到修复，不断积累，最终死亡。目前，美国FDA批准的PARPi包括奥拉帕尼(Olaparib)，鲁卡帕尼(Rucaparib)和尼拉帕尼(Niraparib)，其中奥拉帕尼已在中国被批准上市。随着此类药物的上市，检测BRCA1/2基因突变显得尤为重要：既可以筛查乳腺癌和卵巢癌的高危风险人群，又可以筛选那些潜在的PARPi治疗收益人群。此外，BRCA1/2 基因的突变也参与了其他一些癌症的发生与发展过程，可以作为遗传风险分析因子，如原发性腹膜癌、胰腺癌、***癌等。总之，BRCA1/2基因突变检测的市场前景十分广阔。
BRCA1和BRCA2基因所包含的序列较长，均大于80kb，且不存在热点突变，常规的覆盖少数位点的检测方法不能完整揭示该基因的突变风险。因此，临床上普遍建议采用下一代测序技术(Next-Generation Sequencing，NGS，又称二代测序技术)对BRCA1/2基因进行检测。二代测序技术，是近年来兴起的可以一次对数十万乃至数亿条DNA分子进行测序的高通量检测技术，目前已广泛地应用于产前检测，肿瘤突变分析和用药指导，病原体微生物筛查等领域。与其他技术平台相比，二代测序技术具有检测通量高、速度快、测序长、可检测未知突变等优势，已成为BRCA1/2基因突变检测首选技术平台。
随着临床研究的不断深入，发现不但BRCA1/2胚系突变的肿瘤患者可受益于PARPi类药物，那些肿瘤组织中发生BRCA1/2突变(又称BRCA基因的“体细胞突变”，somaticmutation)的患者同样可以受益于PARPi治疗，其肿瘤无进展生存周期(progression-freesurvival，PFS)与BRCA胚系突变的患者类似。BRCA体细胞突变患者占乳腺癌和卵巢癌患者总数的10％左右，完全可作为肿瘤的用药指导和预后判断因素。有鉴于此，《基于下一代测序技术的BRCA1/2基因检测指南(2019版)》正式将BRCA体细胞突变检测纳入治疗方案评估手段，并列为I级推荐。
然而，目前市场上的产品并不能很好地满足不断提高的BRCA基因突变检测需要。首先，二代测序技术根据其文库构建方式不同，分为探针杂交捕获法和多重扩增法两大类：探针杂交捕获法成本高昂，操作也较为复杂；多重扩增法则需要设计一些相互重叠的扩增片段，而这些重叠片段会相互干扰，因此必须分为2管甚至6-8管进行操作；无论哪种路线的产品操作均比较费时费力。其次，已有的产品特异性、灵敏度普遍不够，需要的样本输入量高，通常需要数百纳克甚至数微克的样本，这对组织样本而言是无法接受的；再次，已有的产品由于其原始设计问题，常不能检测肿瘤组织，尤其是石蜡包埋组织(Formalin-Fixedand Parrffin-Embedded，FFPE)中的体细胞突变；最后，现有的资料表明，一部分BRCA1/2基因突变，无论是胚系还是体细胞突变，属于整个基因或部分外显子的“拷贝数变异”(copynumber variation, CNV)，常规的产品无法有效检测。
发明内容
针对现有技术中存在的不足之处，本发明的目的是提供一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，除了常规的点突变 (SNVs)和短***/缺失(shortin_dels)之外，还可以检测这两个基因的拷贝数变异(CNV)。在样本类型上，它既可以检测基因组DNA样本，又可以检测肿瘤FFPE样本，可满足对于卵巢癌、乳腺癌的遗传风险评估，以及PARPi 抑制剂用药指导，预后评估等目的，整体流程简单高效，具有良好的前景。为了实现根据本发明的上述目的和其他优点，提供了一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，包括：
263对的第一组引物；
每对第一组引物包括正向引物序列与反向引物序列，且所述正向引物序列与反向引物序列均包括可特异性扩增BRCA1/BRCA2基因外显子、内含子或 UTR区域的序列；
所述可特异性扩增BRCA1/BRCA2基因外显子、内含子或UTR区域的序列包括SEQ IDNO.1～526所示的序列，优选的，为其中20-25个碱基。
优选的，还包括20对的第二组引物，且所述第二组引物均包括正向引物与反向引物，所述正向引物与反向引物均包括可特异性扩增人类基因组特定参考区域的序列，作为BRCA1/BRCA2基因拷贝数变异的参考，所述可特异性扩增人类基因组特定参考区域序列均包括SEQ ID NO.527～566所示的序列，优选的，为其中20-25个碱基。
优选的，所述第一组引物、第二组引物的正向引物均包括SEQ ID NO.567 所示的序列，优选的，为其中16-25个碱基。
优选的，所述第一组引物、第二组引物的反向引物均包括SEQ ID NO.568 所示的序列，优选的，为其中16-25个碱基。
一种用于BRCA1和BRCA2基因检测的试剂，包括上文所述的第一组引物、第二组引物。
优选的，还包括三对接头，所述三对接头分别对应Illumina、Ion Torrent、 MGI三种测序平台，且所述三对接头包括与SEQ ID NO.567，SEQ ID NO.568 所述序列配对的DNA片段。
优选的，所述试剂用于来自新鲜或冰冻组织、全血、唾液或FFPE的DNA 样本的分子检测。
优选的，所述试剂用于基于NGS方法的BRCA1和BRCA2基因的检测。
本发明与现有技术相比，其有益效果是：
(1)本发明所述的引物组合物，可用于人类卵巢癌、乳腺癌敏感基因 BRCA1和BRCA2的分子检测，包括点突变、短***/缺失、以及拷贝数变异的检测。检测结果可满足卵巢癌、乳腺癌遗传风险分析，PARPi用药指导，预后评估等需要。
(2)本发明所述的试剂构建文库时为单管反应，操作简单便捷，可在数小时内完成NGS文库构建。扩增子长度为150bp左右，对测序平台的要求较低。测序结果的比对率、中靶率和均一度都较好。
(3)本发明所述的试剂适用于来自受试者的新鲜或冰冻组织、外周血白细胞DNA、唾液DNA、FFPE等多种类型样本的检测，且对样本的要求较低。单次反应的常规样本输入量为20ng，通过优化实验可进一步低至5-10ng，对一定程度片段化的FFPE样本也能有效地检测。检测灵敏度为25％(胚系突变)或2％(体细胞突变)。
(4)本发明所涉及方法简单，总体成本低，填补了国内BRCA1和BRCA2 基因检测领域多重PCR技术构建NGS文库的空白。本发明有助于实现试剂从实验室向临床应用和体外诊断试剂的转化，具有良好的应用前景。
附图说明
图1为根据本发明的基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物的结构示意图；
图2为根据本发明的基于NGS方法检测人类BRCA1和BRCA2基因突变的各标准品和临床样本的CNV检测结果图。
具体实施方式
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
参照图1-2，一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，包括：
263对的第一组引物；
每对第一组引物包括正向引物序列与反向引物序列，且所述正向引物序列与反向引物序列均包括可特异性扩增BRCA1/BRCA2基因外显子、内含子或 UTR区域的序列；
所述可特异性扩增BRCA1/BRCA2基因外显子、内含子或UTR区域序列包括SEQ IDNO.1～526所示的序列，优选的，为其中20-25个碱基。
进一步的，还包括20对的第二组引物，且所述第二组引物中每对引物均包括正向引物与反向引物，所述正向引物与反向引物均包括可特异性扩增人类基因组中特定的参考区域的序列，所述可特异性扩增人类基因组特定参考区域的序列均包括SEQ ID NO.527～566所示的序列，优选的，为其中20-25 个碱基。
进一步的，所述第一组引物、第二组引物正向引物均包括SEQ ID NO.567 所示的序列，且为其中16-25个碱基。
进一步的，所述第一组引物、第二组引物的反向引物均包括SEQ ID NO.568所示的序列，且为其中16-25个碱基。
进一步的，包括所述第一组引物、第二组引物。
进一步的，还包括三对接头，所述三对接头分别对应Illumina、Ion Torrent、 MGI三种测序平台，且所述三对接头包括与权利要求3、4中所述的序列配对的DNA片段。
进一步的，所述试剂用于来自新鲜或冰冻组织、全血、唾液或FFPE的 DNA样本的分子检测。
进一步的，所述试剂用于基于NGS方法的BRCA1和BRCA2基因的检测。
本发明所述的引物组合物共计566条，又分为两组：第一组引物为263 对，其中的正向引物3’端DNA序列分别如SEQ ID 1～263所示，5’端DNA 序列如SEQ ID NO.567所示，反向引物的3’端DNA序列分别如SEQ ID 264～526 所示，5’端DNA序列如SEQ ID NO.568所示；第二组引物为20对，其中的正向引物3’端DNA序列分别如SEQ ID 527～546所示，5’端DNA序列如SEQ ID NO.567所示，反向引物的3’端DNA序列分别如SEQ ID 547～566所示，5’端DNA序列如SEQ ID NO.568所示。
该引物组合物可通过多重PCR的方法，特异性地扩增BRCA1和BRCA2 基因所有外显子区域和至少10bp的内含子毗邻区，以及人类基因组中多个特定的参考位点，可测的突变类型包括点突变，短***/缺失和拷贝数变异。该引物组合物的整体设计使得所有对目的片段的扩增可在同一管中进行，避免了分管的麻烦，节省了相关物料和时间的成本。扩增产物的平均长度在150bp左右，比通常的设计要短，因此该引物组合物既可用于检测来自全血或其他组织的基因组DNA样本，又可用于检测来自肿瘤组织，尤其是FFPE 的DNA样本。
实施例1
本发明多重PCR建库测序流程采取的验证方法如下：
1.样本准备
1.1样本提取
使用Qiagen公司的QIAamp DNA Mini Kit(货号：51304)提取全血临床样本的基因组DNA；使用Qiagen公司的GeneRead DNA FFPE Kit(货号：180134) 提取FFPE临床样本的DNA。
1.2样本质控
使用Thermo Fisher公司的Qubit dsDNA HS Assay Kit(货号：Q32851/ Q32854)对提取的DNA进行定量。各临床样本的SNV/Indel突变交由第三方 NGS检测验证，CNV突变由MLPA验证。
2.文库构建
2.1第一轮基因特异性PCR
2.1.1按下表所示配制基因特异性PCR反应液，每管加入相应的DNA样本。
Figure RE-GDA0003289541980000081
Figure RE-GDA0003289541980000091
2.1.2盖上管盖，震荡混匀后，快速离心。
2.1.3按以下程序运行PCR反应。
Figure RE-GDA0003289541980000092
2.2引物酶解
2.2.1 PCR板/管短暂离心，移除封口或揭开管盖。
2.2.2将核酸外切酶颠倒混匀，离心机上短暂离心。
2.2.3核酸外切酶用无核酸酶水3:2稀释。
2.2.4每管加入5μL外切酶稀释液，并用移液器吹打混匀。
2.2.5将PCR板/管封口，振荡5-10秒，然后短暂离心。
2.2.6按以下程序运行PCR反应。
温度 时间 循环数
37℃ 20分钟 1
80℃ 10分钟 1
8℃ Hold 1
2.3基因特异性PCR产物纯化
2.3.1各管从PCR仪中取出后快速离心将液体甩至管底。
2.3.2每管加入1.2倍体积的纯化磁珠，震荡混匀并快速离心。室温静置 5分钟。
2.3.3将各管转移至磁力架上，室温放置5分钟或至液体澄清。小心吸去上清，注意避免碰到磁珠。
2.3.4每管加入150μL 70％乙醇清洗，孵育30秒后吸去乙醇，注意避免碰到磁珠。重复清洗一次。
2.3.5盖上管盖，将各管快速离心后放置于磁力架上，小心地将管中剩余的乙醇吸净。
2.3.6室温放置2-3分钟至磁珠干燥。各管加入32μL无核酶水，盖上管盖，震荡混匀并快速离心。
2.3.7将各管转移至磁力架上，室温放置5分钟。转移上清至新管中。
2.4第二轮加接头PCR
2.4.1按下表所示配制基因特异性PCR反应液。
Figure RE-GDA0003289541980000101
2.4.2盖上管盖，震荡混匀后，快速离心。
2.4.3按以下程序运行PCR反应。
Figure RE-GDA0003289541980000102
Figure RE-GDA0003289541980000111
2.5文库纯化
2.5.1各管从PCR仪中取出后快速离心将液体甩至管底。
2.5.2每管加入1倍体积的纯化磁珠，震荡混匀并快速离心。室温静置5 分钟。
2.5.3将各管转移至磁力架上，室温放置5分钟或至液体澄清。小心吸去上清，注意避免碰到磁珠。
2.5.4每管加入150μL 70％乙醇清洗，孵育30秒后吸去乙醇，注意避免碰到磁珠。重复清洗一次。
2.5.5盖上管盖，将各管快速离心后放置于磁力架上，小心地将管中剩余的乙醇吸净。
2.5.6室温放置2-3分钟至磁珠干燥。各管加入32μL无核酶水，盖上管盖，震荡混匀并快速离心。
2.5.7将各管转移至磁力架上，室温放置5分钟。转移上清至新管中。
2.6文库质控
使用Qubit对文库进行定量；使用Qseq100对文库片段大小和纯度进行分析。
3.上机测序
3.1将各文库根据浓度统一稀释至5nM，各取少量等体积混合。
3.2加入等体积0.2N NaOH变性，再用HT1溶液稀释至15pM。
3.3取594μL 15pM混合文库，加入6μL 12.5pM PhiX library control。
3.4将上述600μL加样至测序试剂盒，装载入Illumina Miseq测序仪测序。测序读长PE150，测序深度不少于2000x。
4.数据结果
4.1样本设置
4.1.1标准品
各商业化标准品情况见下表。
名称 来源 突变情况
NR2 中国食品药品检定研究院 BRCA2:c.7397T>C
NR5 中国食品药品检定研究院 BRCA1:c.5277+1G>A
NR9 中国食品药品检定研究院 BRCA1:c.981_982delAT
NR22 中国食品药品检定研究院 BRCA2:c.275dupA
NA18949 Coriell BRCA1 exon 14、15缺失
NA14626 Coriell BRCA1 exon 12扩增
NA02718 Coriell BRCA2全基因缺失
NA12878 Coriell 阴性样本
4.1.2临床样本
名称 来源 突变情况
F001 FFPE样本 BRCA2:c.7007G>A
F007 FFPE样本 BRCA1:c.5278-2A>G
F011 FFPE样本 BRCA2:c.8400_8402delinsAAAA
mp19-1 全血样本 BRCA1 exon 1-3缺失
mp19-2 全血样本 BRCA1 exon 2缺失
4.2突变检测结果
4.2.1SNV&Indel检测结果
各标准品和临床样本的SNV&Indel检测结果与参考结果完全一致。
4.2.2 CNV检测结果，如图2。
这里说明的设备数量和处理规模是用来简化本发明的说明的，对本发明的应用、修改和变化对本领域的技术人员来说是显而易见的。
尽管本发明的实施方案已公开如上，但其并不仅限于说明书和实施方式中所列运用，它完全可以被适用于各种适合本发明的领域，对于熟悉本领域的人员而言，可容易地实现另外的修改，因此在不背离权利要求及等同范围所限定的一般概念下，本发明并不限于特定的细节和这里示出与描述的图例。
序列表
<110> 上海真固生物科技有限公司
<120> 一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物及其试剂
<160> 568
<170> SIPOSequenceListing 1.0
<210> 1
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
gcctgacttc cgggtggtgc gtgtgctgcg 30
<210> 2
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 2
accggcgcgg tttttgtcag cttactccgg 30
<210> 3
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 3
ccacccatgc ctgagagaaa ggtccttgcc 30
<210> 4
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 4
agcccctggt aattgctgta ttccgaagac 30
<210> 5
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 5
gcatccctgt gtaagtgcat tttggtcttc 30
<210> 6
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 6
aaaaatgcct attggatcca aagagaggcc 30
<210> 7
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 7
cacagtgttg cttagaacca taaactgttc 30
<210> 8
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 8
acctttctat agattcgcaa gagaatggat 30
<210> 9
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 9
atttgtctgt cactggttaa aactaaggtg 30
<210> 10
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 10
ctgaacctgc agaagaatct gaacataaaa 30
<210> 11
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 11
tataatcagc tggcttcaac tccaataata 30
<210> 12
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 12
caccacacca gcgttttctt tgtagaggca 30
<210> 13
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 13
taaacacttc caaagaatgc aaatttataa 30
<210> 14
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 14
gaaggaatgt tcccaatagt agacataaaa 30
<210> 15
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 15
atattttggc taagagcctg gtagaagatc 30
<210> 16
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 16
ataagataaa ctagtttttg ccagtttttt 30
<210> 17
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 17
catgtaacac cacaaagaga taagtcaggt 30
<210> 18
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 18
gtggtatgtg ggagtttgtt tcatacacca 30
<210> 19
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 19
tatccttaat gatcagggca tttctataaa 30
<210> 20
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 20
tcctcccagg gtcgtcagac accaaaacat 30
<210> 21
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 21
tattagataa tgtctttgat aagtgtgtta 30
<210> 22
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 22
aacctcacag catcatctga ctttccaact 30
<210> 23
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 23
agtagatgtg ctttttgatg tctgacaaaa 30
<210> 24
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 24
atttatctaa gcctttgaga aagtctctaa 30
<210> 25
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 25
ccagctcata tgtctttctc cttcacaatc 30
<210> 26
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 26
tgtgcattga gagtttttat actagtgatt 30
<210> 27
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 27
ttttccaatc atgatgaaag tctgaagaaa 30
<210> 28
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 28
atgcaccacc atgcccaact attgtatttt 30
<210> 29
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 29
ctgtttctat gagaaaggtt gtgagaataa 30
<210> 30
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 30
atttggaaaa acatcaggga attcatttaa 30
<210> 31
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 31
ctagaagatg aagtatatga aacagttgta 30
<210> 32
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 32
aaagtaagaa ctagcaagac taggaaaaaa 30
<210> 33
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 33
atctgaagtg gaaccaaatg atactgatcc 30
<210> 34
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 34
caaggaagtt gtaccgtctt tggcctgtga 30
<210> 35
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 35
cccctattgc atatttcttc atgtgaccaa 30
<210> 36
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 36
agaattcttt gccacgtatt tctagcctac 30
<210> 37
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 37
aagagcagca tcttgaatct catacagact 30
<210> 38
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 38
taagagaatc acctaaagag actttcaatg 30
<210> 39
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 39
gaaagtggac tggaaataca tactgtttgc 30
<210> 40
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 40
accaccacac agaattctgt agctttgaag 30
<210> 41
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 41
aataccgaaa gaccaaaaat cagaactaat 30
<210> 42
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 42
ttgatggtac tttaattttg tcactttgtg 30
<210> 43
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 43
taactagctc ttttgggaca attctgagga 30
<210> 44
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 44
cagaagctga ttctctgtca tgcctgcagg 30
<210> 45
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 45
ttggctgcag catgtcaccc agtacaacat 30
<210> 46
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 46
atgccagcac tcttatttta actcctactt 30
<210> 47
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 47
tcatacaaaa tgtcagacaa gctcaaaggt 30
<210> 48
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 48
ttgagctgtt gccacctgaa aaatacatga 30
<210> 49
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 49
gtcaatccag actctgaaga acttttctca 30
<210> 50
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 50
agacttgact tgtgtaaacg aacccatttt 30
<210> 51
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 51
aaagatttgg tttatgttct tgcagaggag 30
<210> 52
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 52
aaatgggcag gactcttagg tccaatttca 30
<210> 53
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 53
taaggaaatc aagctctctg aacataacat 30
<210> 54
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 54
tgagcaagcc tcagtcaatt aatactgtat 30
<210> 55
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 55
atttaacacc tagccaaaag gcagaaatta 30
<210> 56
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 56
tgcagaagag tacatttgaa gtgcctgaaa 30
<210> 57
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 57
cttctgagga atgcagagat gctgatcttc 30
<210> 58
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 58
gaaaaatgac tgtaacaaaa gtgcttctgg 30
<210> 59
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 59
gtttctactg aagctctgca aaaagctgtg 30
<210> 60
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 60
tatcttcaag taaatgtcat gattctgttg 30
<210> 61
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 61
aaatgactac tggcactttt gttgaagaaa 30
<210> 62
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 62
tgatggcagt gattcaagta aaaatgatac 30
<210> 63
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 63
ttatgaagga gggaaacact cagattaaag 30
<210> 64
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 64
aagaacagtt aactgctact aaaacggagc 30
<210> 65
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 65
aaatattagt gtcgccaaag agtcatttaa 30
<210> 66
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 66
ctgacataag aaagaacaaa atggacattc 30
<210> 67
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 67
atcaactagt gaccttccag ggacaacccg 30
<210> 68
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 68
aacctttttg atgaaaaaga gcaaggtact 30
<210> 69
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 69
aagtacagag aggcctgtaa agaccttgaa 30
<210> 70
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 70
aaaaccttgt ttctattgag actgtggtgc 30
<210> 71
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 71
aacagcaaaa agtcctgcaa cttgttacac 30
<210> 72
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 72
tgagtcagac ttcattactt gaagcaaaaa 30
<210> 73
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 73
tatagctgaa aatgacaaaa atcatctctc 30
<210> 74
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 74
aacagctatt cctaccattc tgatgaggta 30
<210> 75
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 75
atgtaaaaga tgcaaatgca tacccacaaa 30
<210> 76
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 76
ggccacctgc atttaggata gccagtggta 30
<210> 77
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 77
taaatcaaaa atttgccaaa cgaaaattat 30
<210> 78
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 78
cacataaggt ttttgctgac attcagagtg 30
<210> 79
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 79
gaagcttcat aagtcagtct catctgcaaa 30
<210> 80
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 80
tttagcacag caagtggaaa atctgtccag 30
<210> 81
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 81
taaaagtaac gaacattcag accagctcac 30
<210> 82
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 82
aagcaagttt ccattttaga aagttcctta 30
<210> 83
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 83
cacctacgtc tagacaaaat gtatcaaaaa 30
<210> 84
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 84
caaataactt aaatgttgaa ggtggttctt 30
<210> 85
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 85
caatttcaac aagacaaaca acagttggta 30
<210> 86
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 86
agaacaggct tcacctaaaa acgtaaaaat 30
<210> 87
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 87
aagcttttat ggaagatgat gaactgacag 30
<210> 88
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 88
aagtagctta tctgtggtat ctggtagcat 30
<210> 89
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 89
tctatagact tttgagaaat aaaactgata 30
<210> 90
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 90
ggagaaccct caatcaaaag aaacttatta 30
<210> 91
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 91
caagcttaca gagccagata caagcttccc 30
<210> 92
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 92
ttatgctgat ttctgttgta tgcttgtact 30
<210> 93
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 93
gtgtatttac agtaacatgg atattctctt 30
<210> 94
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 94
cctaggcaca ataaaagatc gaagattgtt 30
<210> 95
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 95
agaaagctaa aacctgacac tagagcaaac 30
<210> 96
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 96
tgtagcaaat gagggtctgc aacaaaggca 30
<210> 97
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 97
acagaatcca aattttaccg cacctggtca 30
<210> 98
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 98
gacatccatt ttatcaagtt tctgctacaa 30
<210> 99
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 99
agagttgaac agtgtgttag gaatattaac 30
<210> 100
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 100
tcagtttaac aaaaacaact ccaatcaagc 30
<210> 101
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 101
tatacagctt tacaagttga aacatccttt 30
<210> 102
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 102
tgtgcctggc caggggttgt gctttttaaa 30
<210> 103
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 103
taattacaag tcttcagaat gccagagata 30
<210> 104
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 104
gtctttccac agccaggcag tctgtatctt 30
<210> 105
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 105
gaacacatta gtgcaggaat ggatgaatga 30
<210> 106
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 106
tgttataatt gtttttattg tgtgatacat 30
<210> 107
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 107
tatacgtatg gcgtttctaa acattgcata 30
<210> 108
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 108
ggaaaaggaa tacagttggc tgatggtgga 30
<210> 109
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 109
ttaactgtct ctcgaactaa aaagttggct 30
<210> 110
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 110
gagaatagtt gtagttgttg aattcagtat 30
<210> 111
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 111
ttcagggctc tgtgtgacac tccaggtgtg 30
<210> 112
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 112
catatggaaa ctggcagcta tggaatgtgc 30
<210> 113
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 113
tccctagccc ccatttaaga gagatcacac 30
<210> 114
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 114
gaattctaga gtcacacttc ctaaaatatg 30
<210> 115
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 115
aaaagataat ggaaagggat gacacagctg 30
<210> 116
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 116
agcaataaaa ctagtagtgc agatacccaa 30
<210> 117
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 117
cccctcttag ctgtcttaaa gaatggcaga 30
<210> 118
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 118
ggcagttcta gaagaatgaa aactcttatg 30
<210> 119
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 119
ttaatttgtc cagatttctg ctaacagtac 30
<210> 120
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 120
ttcctctgcc cttatcatcg cttttcagtg 30
<210> 121
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 121
agccactgtg cctggcctga tacaattaac 30
<210> 122
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 122
aatgaaagag aggaagaaaa ggaagcagca 30
<210> 123
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 123
ttttggtgtt agacaaagaa tagcaacaag 30
<210> 124
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 124
gcagtatcta tgaaactcag tgaagtgaaa 30
<210> 125
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 125
ggtgttttat gcttggttct ttagttttag 30
<210> 126
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 126
acgtgcacta acaagacagc aagttcgtgc 30
<210> 127
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 127
gaaatgctgc atttctcaaa agggatgtgt 30
<210> 128
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 128
attggaggat catttttgcc gtagggaaat 30
<210> 129
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 129
tgttctgatt gctttttatt ccaatatctt 30
<210> 130
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 130
aatgttgaat gataagaaac aagctcagat 30
<210> 131
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 131
gaacaaggtt tatcaaggga tgtcacaacc 30
<210> 132
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 132
aatgataatc acttcttcca ttgcatcttt 30
<210> 133
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 133
ctgagtattt ggcgtccatc atcagattta 30
<210> 134
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 134
gaaagagcta acatacagtt agcagcgaca 30
<210> 135
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 135
taagtgcttg ttagtttatg gaatctccat 30
<210> 136
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 136
ttcagcaaat ttttagatcc agactttcag 30
<210> 137
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 137
atattagagt ttcctttctt gcatcttaaa 30
<210> 138
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 138
ttctaggact tgcccctttc gtctatttgt 30
<210> 139
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 139
atgttaattg ctgcaagcaa cctccagtgg 30
<210> 140
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 140
ttttctgcta gtccaaaaga gggccacttt 30
<210> 141
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 141
ccctaaatca ctgatactgg ttttgtaatt 30
<210> 142
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 142
atgtgggttt gcaatttata aagcagcttt 30
<210> 143
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 143
atatactgca tgcaaatgat cccaagtggt 30
<210> 144
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 144
tatatggagg ccatcgtata ttctgttgta 30
<210> 145
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 145
ttactacata attatgatag gctacgtttt 30
<210> 146
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 146
gtatggccaa aaggaagtct gtttccacac 30
<210> 147
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 147
caaaaagaga agagccttgg atttcttgag 30
<210> 148
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 148
aggagttgtg gcaccaaata cgaaacaccc 30
<210> 149
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 149
ttcaatagct gacgaagaac ttgcattgat 30
<210> 150
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 150
tatctcagac tgaaacgacg ttgtactaca 30
<210> 151
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 151
taattttgtg ctcatggcag atttccaagg 30
<210> 152
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 152
ttcttggggt cctgtggctc tgtacctgtg 30
<210> 153
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 153
gggatctggg gtatcaggta ggtgtccagc 30
<210> 154
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 154
gagccaccat gcccggcctt gatttatgtt 30
<210> 155
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 155
aacccatgca aaaggacccc atatagcaca 30
<210> 156
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 156
gctcaacttc acccccggga ttataggcat 30
<210> 157
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 157
taatatgtgc agttctcaaa tccttaccca 30
<210> 158
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 158
acaggagaga atattgtgtc ctccctctct 30
<210> 159
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 159
tacactctcc ggatgaaggc ttatagcaag 30
<210> 160
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 160
tttctcacta acatgttggc actaacagca 30
<210> 161
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 161
acccccatcg tgggatcttg cttataatac 30
<210> 162
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 162
ctttttcttc tgttcaccac ctgatgattt 30
<210> 163
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 163
gctgtgcctg gcccagtgaa cattatataa 30
<210> 164
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 164
cagagtggtg gggtgagatt tttgtcaact 30
<210> 165
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 165
ttgctgggca gagatttgac caaaagcaag 30
<210> 166
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 166
caggctggtc ttgaactccc gacatcaggt 30
<210> 167
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 167
gactgaatga atatctctgg ttagtttgta 30
<210> 168
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 168
aagagcacag gaagaatgac agaggagagg 30
<210> 169
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 169
gttataaaag agacccattt tcccagcatc 30
<210> 170
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 170
tgcaattctg aggtgttaaa gggaggaggg 30
<210> 171
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 171
gaagtgctgt cctaagaagc taaagagcct 30
<210> 172
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 172
cgtgcctggc ccacactcca aatcttaacc 30
<210> 173
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 173
tatgcagcag atgcaaggta ttctgtaaag 30
<210> 174
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 174
tgcacgttct acacgtgtcc tggaactatt 30
<210> 175
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 175
atacctacat aaaactcttt ccagaatgtt 30
<210> 176
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 176
catggacatt cttttgttga ccctttctgt 30
<210> 177
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 177
ctggactctg ggcagattct gcaactttca 30
<210> 178
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 178
aggttgaaga tggtatgttg ccaacacgag 30
<210> 179
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 179
acaccaagac tccctcatcc tcaaaatcat 30
<210> 180
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 180
ttcagagtaa aatcaaagtg tttgttccaa 30
<210> 181
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 181
tccagctgtt gctcctccac atcaacaacc 30
<210> 182
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 182
atctaaccgc acatttctca tgttgtagct 30
<210> 183
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 183
aaataaagat gtcagatacc acagcatctt 30
<210> 184
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 184
atgtttctta cctttccact cctggttctt 30
<210> 185
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 185
gctttgttct gatagtgata attcaggtta 30
<210> 186
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 186
ctgccttggg tccctctgac tggtatatta 30
<210> 187
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 187
agataaaggg gaaggaaaga attttgctta 30
<210> 188
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 188
gtgctttgtt ctggatttcg caggtcctca 30
<210> 189
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 189
tagaaggctg gctcccatgc tgttctaaca 30
<210> 190
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 190
ctagtatgga actacaagtt ctagctgaac 30
<210> 191
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 191
gctacaatga gctaggcagt gtatgtgtag 30
<210> 192
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 192
gaatgcaaag gacaccacac acacgcatgt 30
<210> 193
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 193
tctcattggc aggactggat ttactttcat 30
<210> 194
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 194
ataaatagac tggggcaaac acaaaaacct 30
<210> 195
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 195
tgaatccatg ctttgctctt cttgattatt 30
<210> 196
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 196
ctttcagact gatgcctcat ttgtttggaa 30
<210> 197
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 197
aagaaaacaa gctagcagaa cattttgttt 30
<210> 198
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 198
atgcctttgc caatattacc tggttactgc 30
<210> 199
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 199
tcggtagcaa cggtgctatg cctagtagac 30
<210> 200
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 200
cagggaagct cttcatcctc actagataag 30
<210> 201
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 201
cttcggtaac cctgagccaa atgtgtatgg 30
<210> 202
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 202
aacagcagaa ctttccttaa tgtcattttc 30
<210> 203
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 203
tcatcaggtg tctcagaaca aacctgagat 30
<210> 204
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 204
tggagagaaa tctgtattaa cagtctgaac 30
<210> 205
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 205
cttccaggaa gactttgttt atagacctca 30
<210> 206
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 206
ttctacctag ttctgcttga atgttttcat 30
<210> 207
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 207
ggagcccact tcattagtac tggaacctac 30
<210> 208
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 208
tcatttccca tttctctttc aggtgacatt 30
<210> 209
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 209
ttgatgggaa aaagtggtgg tatacgatat 30
<210> 210
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 210
agatgataga caaaacctag agcctccttt 30
<210> 211
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 211
acaggcttga tattagactc attctttcct 30
<210> 212
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 212
cattcctctt ctgcatttcc tggatttgaa 30
<210> 213
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 213
gagcatcaag ttcactttct tccatttcta 30
<210> 214
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 214
aaccatgaat tagtcccttg gggttttcaa 30
<210> 215
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 215
tttgccttcc ctagagtgct aacttccagt 30
<210> 216
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 216
cagatctttc agtttgcaaa accctttctc 30
<210> 217
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 217
cttggaaggc taggattgac aaattcttta 30
<210> 218
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 218
gcatttgtta acttcagctc tgggaaagta 30
<210> 219
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 219
cttggctcca gttgcaggtt ctttaccttc 30
<210> 220
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 220
aattcagtac aattaggtgg gcttagattt 30
<210> 221
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 221
agaagacttc ctcctcagcc tattcttttt 30
<210> 222
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 222
tcagctttcg ttttgaaagc agattctttt 30
<210> 223
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 223
tagaatcacc ttttgtttta ttctcatgac 30
<210> 224
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 224
ttgaactgcc aaatctgctt tcttgataaa 30
<210> 225
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 225
gggacgctct tgtattatct gtggctcagt 30
<210> 226
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 226
tggggaggct tgccttcttc cgataggttt 30
<210> 227
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 227
tgaggatcac tggccagtaa gtctattttc 30
<210> 228
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 228
ctccccatca tgtgagtcat cagaacctaa 30
<210> 229
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 229
agtatctcta ggattctctg agcatggcag 30
<210> 230
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 230
tctgtgctgg gagtccgcct atcattacat 30
<210> 231
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 231
cttgctaagc caggctgttt gcttttatta 30
<210> 232
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 232
gttctcatgc tgtaatgagc tggcatgagt 30
<210> 233
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 233
ctgcacgctt ctcagtggtg ttcaaatcat 30
<210> 234
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 234
cgaaagggca acaatcagtt acagaaggtc 30
<210> 235
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 235
ctctcttttc agtgcctgtt aagttggcaa 30
<210> 236
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 236
agaggtgaga tcacacctct gtactccagc 30
<210> 237
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 237
gaagcaagca tgtgcagagt tctactgtta 30
<210> 238
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 238
gaaaatacca gcttcataga caaaggttct 30
<210> 239
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 239
aaagcacaat agtgcctgga atatagttaa 30
<210> 240
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 240
tgcctggcct cttttgctcc ctttttaaag 30
<210> 241
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 241
ctataagata aggaatccag caattattat 30
<210> 242
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 242
gtagacagac gtcttttgag gttgtatccg 30
<210> 243
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 243
ttgggaggct ggagcaggac tgcttgaggc 30
<210> 244
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 244
tataccatct ttccttcttc actaactttt 30
<210> 245
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 245
gctaaggcag gaggactgct tctagcctgg 30
<210> 246
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 246
aaatggtttt accaaggaag gattttcggg 30
<210> 247
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 247
cttcatcttt tagatgttca ggagagttat 30
<210> 248
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 248
aaccctatgt atgctctttg ttgtgttaag 30
<210> 249
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 249
aagatgtgaa aaatgtgcac cttacgattg 30
<210> 250
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 250
tcctgagttt tcatggacag cacttgagtg 30
<210> 251
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 251
aagatgcagc aacagtagaa aacctctata 30
<210> 252
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 252
tataaggcag atgtcccata aaactttcag 30
<210> 253
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 253
atttctactt tttcctactg tggttgcttc 30
<210> 254
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 254
gttatatcat tcttacataa aggacactgt 30
<210> 255
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 255
aaagataatc tcacaactgc ccttaagagc 30
<210> 256
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 256
gaaactggta tcaggtcctt tcctgtcttc 30
<210> 257
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 257
taaagttagg tgtttcctgg gttatgaagg 30
<210> 258
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 258
agagaatacg atccttacct tcagtgagct 30
<210> 259
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 259
ggagaggcag agtggatgga gaacaaggaa 30
<210> 260
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 260
aatcatagga atcccaaatt aatacactct 30
<210> 261
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 261
gtttttaaag acatagtgtc cccctcaagg 30
<210> 262
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 262
ggggcccagt tatctgagaa accccacagc 30
<210> 263
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 263
gccctctagc ctctactctt ccagttgcgg 30
<210> 264
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 264
tcccggcggc gacccaccgc cgcaaaagac 30
<210> 265
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 265
cgccacgctg gactgggact gcggaagacg 30
<210> 266
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 266
gacagctggg agggaagtta agtcttgagc 30
<210> 267
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 267
acgtggctag tgcgactaga gaagaggatt 30
<210> 268
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 268
aatacctgct ttgttgcagc gtgtcttaaa 30
<210> 269
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 269
gcaacactgt gacgtactgg gtttttagca 30
<210> 270
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 270
gctagtcaag gggccagttt cctctttatc 30
<210> 271
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 271
acaaatttgt gacccagaac agttaaagat 30
<210> 272
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 272
gtggagtttt aaataggttt ggttcgtaat 30
<210> 273
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 273
ttacaggaga ttggtacagc ggcagagtca 30
<210> 274
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 274
gctaagattt taacacaggt ttgcctaaat 30
<210> 275
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 275
gaagaggttt ttcagtttgc tgattacccc 30
<210> 276
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 276
atagcttcat catacctttc actaagacaa 30
<210> 277
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 277
aacaaatata tgtaggaaaa tgtttcattt 30
<210> 278
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 278
aaaccagcca attcaacatc acaagaacaa 30
<210> 279
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 279
tctagtattc taagaataaa aagcattgtt 30
<210> 280
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 280
taaaccaggt agaatattta ccttcacaaa 30
<210> 281
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 281
aaatctcagg gcaaaggtat aacgctattg 30
<210> 282
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 282
tgggtggtgt agctaaagaa cttgaccaag 30
<210> 283
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 283
atcaacctca tctgctcttt cttgtaaata 30
<210> 284
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 284
aaacataatg cttgacacca ctggactacc 30
<210> 285
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 285
gtaattagaa aggtcaatca acacagtgaa 30
<210> 286
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 286
tgttagcaat ttcaacagtc taatcaatgt 30
<210> 287
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 287
ctaaggtttc aagcattcac tgggggatat 30
<210> 288
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 288
aaatatgcca aaaagaagcc ataaagtgct 30
<210> 289
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 289
tgacttgcag cttctctttg atttgtgttt 30
<210> 290
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 290
gggtgacaga gcaagactcc acctcaaaaa 30
<210> 291
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 291
taagaaaaga ggagcaatcc ttcaatggtg 30
<210> 292
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 292
tggcattgac tttccaatgt ggtctttgca 30
<210> 293
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 293
cataatgaaa aactatcttc ttcagaggta 30
<210> 294
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 294
tttagatttt tcacattcat cagcgtttgc 30
<210> 295
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 295
ttgtcacttc cactctcaaa gggcttctga 30
<210> 296
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 296
ttagacctga aagggttagt tgagaccatt 30
<210> 297
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 297
cttttgttct ctgtgtctaa taggtctttt 30
<210> 298
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 298
atttaccact gtttcctcat ttaatggctt 30
<210> 299
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 299
tgaagaagcc actggagaag ttccagatat 30
<210> 300
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 300
gtttggatca gtcatatgac ctgaaaaact 30
<210> 301
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 301
tggtggctgg ccagcttcca ttatcaatta 30
<210> 302
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 302
tctttttcaa agtggatatt aaacctgcat 30
<210> 303
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 303
aagcatttgc ttcaaactgg gctgaacagt 30
<210> 304
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 304
aaaaaacaca gaaggaatcg tcatctataa 30
<210> 305
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 305
ttataatcaa gatcctgaga gattactgta 30
<210> 306
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 306
cttttttgct ttttggatca ttttcacact 30
<210> 307
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 307
aaagactttt ctgggattga aagtcagtat 30
<210> 308
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 308
aatcatgact aggtttgaca gaacatcctt 30
<210> 309
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 309
gcacatacat cttgattctt ttccatggga 30
<210> 310
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 310
attgtacctt tcttgaaggt gatgctactc 30
<210> 311
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 311
ttattccttt cattagctac ttggaagaca 30
<210> 312
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 312
attgacactt gggttgcttg tttatcacct 30
<210> 313
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 313
gatgtccgat tttaaatctt gacctagagt 30
<210> 314
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 314
agctgttctg aagctacctc caaaactgtg 30
<210> 315
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 315
tttcaacaca agctaaacta gtaggatatt 30
<210> 316
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 316
ttacaatcag aaacaactac actactctgt 30
<210> 317
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 317
attcaaactg acttcctgat tcttctaata 30
<210> 318
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 318
catctctgca ttcctcagaa gtggtcttta 30
<210> 319
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 319
tttcaactgt accttcaaat tgcttgctgc 30
<210> 320
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 320
gaataaaagc ccctaaaccc cacttcattt 30
<210> 321
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 321
gatgtacctc tgcagaagtt tcctcactaa 30
<210> 322
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 322
cacttacagt tttatcatta tgattttcta 30
<210> 323
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 323
ttttcagtat ttctcttgta attttcagta 30
<210> 324
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 324
agcaagtccg tttcatcttt atgaatacaa 30
<210> 325
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 325
acatgcttct tgagctttcg caacttccaa 30
<210> 326
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 326
tctttggcga cactaatatt tttcccactt 30
<210> 327
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 327
ggaaaagtta tgcaattctt ctggtttctg 30
<210> 328
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 328
tgatttccag taccaactgg gacactttct 30
<210> 329
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 329
aacttttttc ccgctagctg tatgaaaacc 30
<210> 330
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 330
ggtctttgcc cattgatggc taaaactggt 30
<210> 331
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 331
gcagctgtga tctcaatggt ctcacatgct 30
<210> 332
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 332
tttgatgttt tgagattttc agtttgtcta 30
<210> 333
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 333
aattttcaat gactgaataa ggggactgat 30
<210> 334
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 334
cagtatttat tctttctggt tgaccatcaa 30
<210> 335
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 335
ggaatagctg ttagacatgc tactgttact 30
<210> 336
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 336
gatcttcaac attcttcaat actggctcaa 30
<210> 337
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 337
agctagtcac aagttcctca acgcaaatat 30
<210> 338
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 338
tttaattgtt tcatgtgaaa cacaaacgat 30
<210> 339
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 339
atatcctctg aatcatccaa tgcctcgtaa 30
<210> 340
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 340
tattttagaa actttctcca atccagacat 30
<210> 341
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 341
tttccacttg ctgtgctaaa aatcccacaa 30
<210> 342
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 342
aacaatactt tggaaaagac ttgcttggta 30
<210> 343
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 343
aaagcctttt tgggatatta aatgttctgg 30
<210> 344
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 344
ttaaatcaaa ttcctctaac actcccttaa 30
<210> 345
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 345
cacagtgctc tgggtttctc ttatcaacac 30
<210> 346
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 346
tcttgttgaa attgagagag atatggagaa 30
<210> 347
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 347
gtgaagcctg ttcttttccc aaaacatgaa 30
<210> 348
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 348
cacaggaaca tcagaaaaag tttcagtttt 30
<210> 349
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 349
atgtgtggca tgacttggca gtttagaatc 30
<210> 350
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 350
ttaaaaatag tgattggcaa cacgaaaggt 30
<210> 351
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 351
aacaaatagt gacctaatgt ttgaaagagt 30
<210> 352
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 352
taattttacc atctggagtg ctttttgaag 30
<210> 353
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 353
accataccta tagagggaga acagatataa 30
<210> 354
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 354
ttattcagac ttgggtattc ggcagacatc 30
<210> 355
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 355
attataggct ttacaatttt cagtgaatgt 30
<210> 356
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 356
agggtacaca ggtaatcggc tctaaagaaa 30
<210> 357
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 357
acttttctca tactgtatta gaatttagaa 30
<210> 358
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 358
gcctatttgc agtgggggag gaaatataga 30
<210> 359
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 359
tcagatgttc atacaaatga gatttagaca 30
<210> 360
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 360
aagactttgg ttggtctgcc tgtagtaatc 30
<210> 361
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 361
aatgttttgc ttttgtctgt tttcctccaa 30
<210> 362
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 362
tcttcttcac actttgtgaa agttacagct 30
<210> 363
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 363
ggggaaaacc atcaggacat tatttaacaa 30
<210> 364
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 364
aaaaaaaaaa gcgcaagccc tgttttataa 30
<210> 365
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 365
aaagacgcgt tgcctttgtt tcttcttaat 30
<210> 366
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 366
acacatacct gtttatgaga acacgcagag 30
<210> 367
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 367
cactctgtca taaaagccat cagtattgta 30
<210> 368
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 368
gaagtagtaa tttggaagca cagatcactt 30
<210> 369
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 369
gtgaaactga aaagactctg catttttgct 30
<210> 370
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 370
aaaattcttc ttttccagcc tttccatcat 30
<210> 371
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 371
taagagaaga aagagggatg agggaataca 30
<210> 372
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 372
agaaaatcca gttcattaaa ccccaggaca 30
<210> 373
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 373
tagctgccag tttccatatg atccatctat 30
<210> 374
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 374
gtattttagt tgaagaagca ccctttctgg 30
<210> 375
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 375
attttgacat ggaagtcaca gactacacag 30
<210> 376
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 376
ttattagcaa tccccaaata gtggatcaaa 30
<210> 377
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 377
aaattatgtc agaaacacag agaacaagtg 30
<210> 378
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 378
cataccaccc atctgtaagt tcaataatgg 30
<210> 379
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 379
caggagagcc caccagttct gctccatgaa 30
<210> 380
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 380
atcaatgact gatttttacc aagagtgcaa 30
<210> 381
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 381
aagggcagag gaaaaggtct agggtcagga 30
<210> 382
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 382
ttcaagaata catcatacct gtatagggta 30
<210> 383
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 383
tgtatcaaaa gaaagaaata tatggtaagt 30
<210> 384
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 384
agtctctttt gttgggcctc cacatatttt 30
<210> 385
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 385
ttctcatatt agaaataaca atgtgtacca 30
<210> 386
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 386
ataactacca tgacaaaaat gcaaaagcca 30
<210> 387
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 387
catgacacct ggcctgctat aagttttcca 30
<210> 388
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 388
cactgcttca taaagctctg caccatcttg 30
<210> 389
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 389
atggccagag agtctaaaac agcttctcac 30
<210> 390
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 390
aaattctaga accctcccat cctctactcg 30
<210> 391
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 391
gagctcatct taagggtgtg gttaatgtaa 30
<210> 392
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 392
tgataaacct tgttcctttt gttcagcaga 30
<210> 393
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 393
taaaaacaaa gcatttacat acttacctga 30
<210> 394
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 394
ttataatcat tttgttagta aggtcatttt 30
<210> 395
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 395
agatgataaa ttctgtatct ctttccttct 30
<210> 396
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 396
ttacaatgaa aggtttgtac cggtagttgt 30
<210> 397
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 397
tcatctgaaa cctacagaaa acaaaacaaa 30
<210> 398
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 398
aatcctatta ggtccacctc agaacaagat 30
<210> 399
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 399
ccaactaatc ataagagatt ttaaaagaca 30
<210> 400
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 400
gaggcttaat aatgtcctca ttaaggtcta 30
<210> 401
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 401
aaataaagta agaaggcctg atttggattc 30
<210> 402
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 402
aaatgtgtgg tgatgctgaa aagtaacctt 30
<210> 403
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 403
agctatttcc ttgatactgg actgtcaaaa 30
<210> 404
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 404
ttaaaagcag aaatgaaaag tttggaccaa 30
<210> 405
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 405
cctgtaccag gaatgatttg agcagtgtac 30
<210> 406
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 406
tatatacttc ttataatatt ccttgagttt 30
<210> 407
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 407
ttggaaagtg tgcacccaga gtttcatatc 30
<210> 408
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 408
tcaatctctt tctccccttt acaagacttt 30
<210> 409
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 409
gtacaaatgg gactaacagg tggaggtaaa 30
<210> 410
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 410
taaatggagt catctgagga gaattcagtt 30
<210> 411
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 411
aaattgtttt tctcctgttg aaccagacaa 30
<210> 412
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 412
tggcctggga actctcctgt tctttgatca 30
<210> 413
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 413
cttataaact ggaaaggtta agcgtcaata 30
<210> 414
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 414
ttcagtcctt ctactgtcct ggctactaaa 30
<210> 415
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 415
ttctctgtct ccagcaattg ggcagatgtg 30
<210> 416
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 416
agtcgattga ttagagccta gtccaggaga 30
<210> 417
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 417
gcttgagtag cacagttctt ggcacatttt 30
<210> 418
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 418
ctttgacact ttgaatgctc tttccttcct 30
<210> 419
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 419
gtgccccacc cctgtaatca caacactggg 30
<210> 420
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 420
agtccctatg gatgccccta ctgtagcctc 30
<210> 421
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 421
gggcctgggt taagtatgca gattactgca 30
<210> 422
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 422
gagtacatgg catatcagtg gcaaattgac 30
<210> 423
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 423
tctaccactc tccaaacaaa acagcaccta 30
<210> 424
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 424
tctctccatt cccctgtccc tctctcttcc 30
<210> 425
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 425
aatgcctgta gagtgcaggt caactacata 30
<210> 426
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 426
gtgcgatctc agctcactga aaactcatct 30
<210> 427
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 427
gcttctcttt ctcttatcct gatgggttgt 30
<210> 428
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 428
aaaagccgat tgttaagttc tgcacacaaa 30
<210> 429
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 429
cttccatcaa tgcaccactt tccttaacaa 30
<210> 430
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 430
cctgtgctct tttgtgaatc gctgacctct 30
<210> 431
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 431
atttatttaa tagtcggcag gaatccatgt 30
<210> 432
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 432
tgctgatgct gagtctgagt taccaaaagg 30
<210> 433
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 433
attttgtttt caacttctaa tcctttgagt 30
<210> 434
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 434
ttaacttcat atcagcctcc cctagacttc 30
<210> 435
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 435
aaaaataaga atatttgttg agcatagcat 30
<210> 436
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 436
tgtgtgctag aggtaactca tgataatgga 30
<210> 437
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 437
aaatgtaccc tttctgagaa aaacagagac 30
<210> 438
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 438
caatggaaga aagtgtgagc agggagaagc 30
<210> 439
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 439
gcaacatacc atcttcaacc tctgcattga 30
<210> 440
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 440
ggaatcagcc tcttctctga tgaccctgaa 30
<210> 441
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 441
cattcatcgt tgtgtaaatt aaacttctcc 30
<210> 442
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 442
agatgtacta gtctatcatg gacacttttg 30
<210> 443
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 443
aaactaccca tctcaagagg agctcattaa 30
<210> 444
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 444
ctttcacaat tggtggcgat ggttttctcc 30
<210> 445
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 445
ggcatttata cacttttatt gctctaggat 30
<210> 446
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 446
gaaaagtagt gaatacccta taagccagaa 30
<210> 447
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 447
acaaagcagt aaagtagatt tgttttctca 30
<210> 448
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 448
ttgaattcca tttggtgcta gcatctgtct 30
<210> 449
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 449
tttccttccc ctttatctcc ttctgaagag 30
<210> 450
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 450
cagccttcta acagctaccc ttccatcata 30
<210> 451
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 451
tttttgaagc agagggatac catgcaacat 30
<210> 452
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 452
agcttctcaa agtatttcat tttcttggtg 30
<210> 453
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 453
aataacatca agtctatttg ggggaatttg 30
<210> 454
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 454
ggcacaattg tttgtggttc attgtctcct 30
<210> 455
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 455
tagtctgctt ttacatctga acctctgttt 30
<210> 456
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 456
cagctaggac gtcatctttg actgaatgag 30
<210> 457
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 457
cagggagttg gtctgagtga caaggaattg 30
<210> 458
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 458
cttgactgca aatacaaaca cccaggatcc 30
<210> 459
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 459
ggcaaaggca tctcaggaac atcaccttag 30
<210> 460
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 460
accgttgcta ccgagtgtct gtctaagaac 30
<210> 461
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 461
gagcttccct gcttccaaca cttgttattt 30
<210> 462
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 462
accgaagagg ggccaagaaa ttagagtcct 30
<210> 463
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 463
agttctgctg tttttagcaa aagcgtccag 30
<210> 464
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 464
agacacctga tgacctgtta gatgatggtg 30
<210> 465
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 465
tcagataact tagaacagcc tatgggaagt 30
<210> 466
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 466
gtcttcctgg aagtaattgt aagcatcctg 30
<210> 467
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 467
gaactaggta gaaacagagg gccaaaattg 30
<210> 468
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 468
taatgaagtg ggctccagta ttaatgaaat 30
<210> 469
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 469
caagtacagt gagcacaatt agccgtaata 30
<210> 470
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 470
aaatctgcta gaggaaaact ttgaggaaca 30
<210> 471
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 471
gttcagaggc aacgaaactg gactcattac 30
<210> 472
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 472
gctttcctgt ggttggtcag aaagataagc 30
<210> 473
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 473
agtccaaaag tcacttttga atgtgaacaa 30
<210> 474
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 474
acattcaagg tttcaaagcg ccagtcattt 30
<210> 475
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 475
aaatgacaca gaaggcttta agtatccatt 30
<210> 476
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 476
accaaataaa tgtgtgagtc agtgtgcagc 30
<210> 477
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 477
ttggtacctg gtactgatta tggcactcag 30
<210> 478
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 478
agtgtctaat aatgctgaag accccaaaga 30
<210> 479
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 479
aatgcacctg gttcttttac taagtgttca 30
<210> 480
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 480
ctggagccaa gaagagtaac aagccaaatg 30
<210> 481
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 481
aaatgccagt caggcacagc agaaacctac 30
<210> 482
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 482
tattcatgcg cttgaactag tagtcagtag 30
<210> 483
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 483
gaaagctgaa cctataagca gcagtataag 30
<210> 484
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 484
aaaaatccta acccaataga atcactcgaa 30
<210> 485
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 485
tcagggaact aaccaaacgg agcagaatgg 30
<210> 486
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 486
agcgtaaaag gagacctaca tcaggccttc 30
<210> 487
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 487
agcctcccca acttaagcca tgtaactgaa 30
<210> 488
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 488
aaaagtgaaa gagttcactc caaatcagta 30
<210> 489
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 489
tattggacgt tctaaatgag gtagatgaat 30
<210> 490
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 490
gttaatgagt ggttttccag aagtgatgaa 30
<210> 491
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 491
tgctgatccc ctgtgtgaga gaaaagaatg 30
<210> 492
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 492
cctggcttag caaggagcca acataacaga 30
<210> 493
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 493
tcattacagc atgagaacag cagtttatta 30
<210> 494
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 494
tatcagggta gttctgtttc aaacttgcat 30
<210> 495
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 495
tctgagacgg atgtaacaaa tactgaacat 30
<210> 496
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 496
ttccacctcc aaggtgtatg aagtatgtat 30
<210> 497
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 497
atttgggacc tttacaaccc acaaaatctc 30
<210> 498
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 498
tgacagttct gcatacatgt aactagtgtt 30
<210> 499
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 499
ctggttcaag cgattctcct gtctcaatct 30
<210> 500
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 500
tcaagtaggc tggtgtttct ccagactaga 30
<210> 501
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 501
ctcagtaaat atttctagtt gaatatctgt 30
<210> 502
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 502
ttgagctccc aggcaccacc atctatttat 30
<210> 503
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 503
gtggtaggca gctttgggaa gtgaatttta 30
<210> 504
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 504
cttggaactg tgagaactct gaggacaaag 30
<210> 505
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 505
tctcttcagg aggaaaagca cagaactggc 30
<210> 506
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 506
aaaaattcaa atcacacata tcccacacat 30
<210> 507
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 507
gtgcccggcc ggtaaaacca ttttcattta 30
<210> 508
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 508
aaaccgtgcc aaaagacttc tacagagtga 30
<210> 509
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 509
gttttccctt gtattttaca gatgcaaaca 30
<210> 510
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 510
catacatagg gtttctcttg gtttctttga 30
<210> 511
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 511
ggactctaaa taactttgat ggtctgtgta 30
<210> 512
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 512
aaaactcagg aagtttgcac aattactttc 30
<210> 513
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 513
taatcacttg ctgagtgtgt ttctcaaaca 30
<210> 514
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 514
caatgcatta tatctgctgt ggatttaggg 30
<210> 515
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 515
tataatttgg taatgatgct aggttggaag 30
<210> 516
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 516
ttgcatgctg aaacttctca accagaagaa 30
<210> 517
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 517
tatcttttca tggctatttg ccttttgagt 30
<210> 518
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 518
tatgtgatca taacagtaag ccatatgcat 30
<210> 519
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 519
cattgtgttt gagccttccc agtttctcct 30
<210> 520
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 520
attgagcctc atttattttc tttttctccc 30
<210> 521
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 521
tgtgcaccca cagtgatagt gcagaaaaat 30
<210> 522
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 522
atctcctatg caaatgaaca gaattgacct 30
<210> 523
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 523
atgtgttaaa gttcattgga acagaaagaa 30
<210> 524
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 524
gggtaggggc ggaacctgag aggcgtaagg 30
<210> 525
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 525
atttcgtatt ctgagaggct gctgcttagc 30
<210> 526
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 526
aaactggaga cctccattag ggcggaaaga 30
<210> 527
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 527
ggagaggtcc ttgcacatgc ttctttattt 30
<210> 528
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 528
tggttggaca ttgcataaaa gaggtccata 30
<210> 529
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 529
gtggaagtgc caaccagcag aagttagatg 30
<210> 530
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 530
gccttggagt atttccccac tctgagactc 30
<210> 531
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 531
cctctccagc ctgtaacaac ttttggtttc 30
<210> 532
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 532
cgcgttggag aatcggacct gacattttcc 30
<210> 533
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 533
gcaatctgca tgccactttt ctttctcaac 30
<210> 534
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 534
ctatttggga gactggtagt gcaaattaaa 30
<210> 535
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 535
tgtggaggca atccatctca tctcacagtg 30
<210> 536
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 536
agacactcac ttggttcgga agtcatctgc 30
<210> 537
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 537
tttcttcatc taaccctttg gggtttactc 30
<210> 538
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 538
acagcacagc cagtttgata cgcttgtttt 30
<210> 539
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 539
tttcctttat ccagctgggg gtataaataa 30
<210> 540
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 540
agctagaagc cttcatgcac atttctactt 30
<210> 541
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 541
gaaaggagga agatggtagc accaaagaag 30
<210> 542
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 542
aggaagtgag aagcatcagg ttgagaatat 30
<210> 543
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 543
aacttcctga cccaataggg tagttttctc 30
<210> 544
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 544
tggttttgac actaccgtgc ttgtgataag 30
<210> 545
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 545
ggcaaaactt cctgacactg gagctaaata 30
<210> 546
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 546
tactctggca cacactcctg acagataata 30
<210> 547
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 547
ggggctgggc attcttgaat acctcatcca 30
<210> 548
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 548
gagcgcagtt gaattcttct gggtgccaag 30
<210> 549
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 549
tagctgcatc tctcctttgt cctcctgtgt 30
<210> 550
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 550
ctagtgttcc tcaacacctt gtccaagaac 30
<210> 551
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 551
tgtagttcac agtcaatagc ggactgtagg 30
<210> 552
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 552
cccttaggaa gtcatggaga ctcccaattt 30
<210> 553
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 553
ctctcatccc tatagtttta caagacagca 30
<210> 554
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 554
tgggcgaaaa cagaacaact gtgtttcctt 30
<210> 555
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 555
taaatttaca gcgtcaggag ggactatatt 30
<210> 556
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 556
cagtgcctgt gtgttgaact ggtaccaacc 30
<210> 557
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 557
tatattcttc tatggggcct gcatttgttt 30
<210> 558
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 558
ccacattttt ggaagtccag gtttcatccc 30
<210> 559
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 559
tcaagtagta gaacacagcc atcttttccc 30
<210> 560
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 560
cgatgttcat aacagcaaaa tccataggct 30
<210> 561
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 561
tgtcccggaa ccgtctcctt tcaaatccta 30
<210> 562
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 562
tgcgatcctt atttgcagat gctgagcaag 30
<210> 563
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 563
cctgctggag ctagatgggg gtacaggagc 30
<210> 564
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 564
aacatgtcat gacttcgcct ctaagcacac 30
<210> 565
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 565
catccctctc cagggcttgt tttcaaatca 30
<210> 566
<211> 30
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 566
gcaagtgtca catgcaaagt actcatccgc 30
<210> 567
<211> 34
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 567
gtgactggag ttcagacgtg tgctcttccg atct 34
<210> 568
<211> 33
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 568
tcgtcggcag cgtcagatgt gtataagaga cag 33

Claims (8)

1.一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，其特征在于，包括：
263对的第一组引物；
每对第一组引物包括正向引物序列与反向引物序列，且所述正向引物序列与反向引物序列均包括可特异性扩增BRCA1/BRCA2基因外显子、内含子或UTR区域的序列；
所述可特异性扩增BRCA1/BRCA2基因外显子、内含子或UTR区域序列包括SEQ ID NO.1～526所示的序列，优选的，为其中20-25个碱基。
2.如权利要求1所述的一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，其特征在于，还包括20对的第二组引物，且所述第二组引物中每对引物均包括正向引物与反向引物，所述正向引物与反向引物均包括可特异性扩增人类基因组中特定的参考区域的序列，所述可特异性扩增人类基因组特定参考区域的序列包括SEQ ID NO.527～566所示的序列，优选的，为其中20-25个碱基。
3.如权利要求1-2所述的一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，其特征在于，所述第一组引物、第二组引物正向引物均包括SEQ ID NO.567所示的序列，优选的，为其中16-25个碱基。
4.如权利要求1-2所述的一种基于NGS方法检测人类BRCA1和BRCA2基因突变的引物组合物，其特征在于，所述第一组引物、第二组引物的反向引物均包括SEQ ID NO.568所示的序列，优选的，为其中16-25个碱基。
5.一种用于BRCA1和BRCA2基因检测的试剂，其特征在于，包括权利要求1-4所述第一组引物、第二组引物。
6.如权利要求5所述的一种用于BRCA1和BRCA2基因检测的试剂，其特征在于，还包括三对接头，所述三对接头分别对应Illumina、Ion Torrent、MGI三种测序平台，且所述三对接头包括与权利要求3、4中所述的序列配对的DNA片段。
7.如权利要求5-6所述的一种用于BRCA1和BRCA2基因检测的试剂，其特征在于，所述试剂用于来自新鲜或冰冻组织、全血、唾液或FFPE的DNA样本的分子检测。
8.如权利要求5-6所述的一种用于BRCA1和BRCA2基因检测的试剂，其特征在于，所述试剂用于基于NGS方法的BRCA1和BRCA2基因的检测。
CN202110723978.0A 2021-06-29 2021-06-29 一种基于ngs方法检测人类brca1和brca2基因突变的引物组合物及其试剂 Active CN113604565B (zh)

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