CN113469864A

CN113469864A - Method and device for acquiring microscopic image

Info

Publication number: CN113469864A
Application number: CN202110716265.1A
Authority: CN
Inventors: 张大庆
Original assignee: Pinghu Laidun Optical Instrument Manufacturing Co ltd
Current assignee: Pinghu Laidun Optical Instrument Manufacturing Co ltd
Priority date: 2021-06-28
Filing date: 2021-06-28
Publication date: 2021-10-01
Anticipated expiration: 2041-06-28
Also published as: CN113469864B

Abstract

The purpose of the present application is to provide a method and apparatus for acquiring a microscopic image, specifically comprising: acquiring current fluorescence parameter information currently used by the microscopic equipment, wherein the fluorescence parameter information is used for determining the corresponding current excitation light wavelength; sending an image acquisition instruction to the microscopic equipment; and receiving a microscopic image sequence which is shot by the microscopic equipment based on the image acquisition instruction and is about to the sample so as to acquire a corresponding microscopic image, wherein the microscopic image sequence comprises microscopic sub-images respectively corresponding to one or more sub-areas of the sample, the microscopic sub-images are used for splicing to obtain the microscopic image, and the microscopic sub-images correspond to the current excitation light wavelength. According to the method and the device, more detailed, concrete and complete images or research data can be obtained, and good use experience is provided for users.

Description

Method and device for acquiring microscopic image

Technical Field

The present application relates to the field of communications, and more particularly, to a technique for acquiring microscopic images.

Background

Microscopic Optical imaging, also commonly referred to as "Optical Microscopy," or "Light Microscopy," refers to a technique whereby visible Light transmitted through or reflected from a sample is passed through one or more lenses to produce a magnified image of the microscopic sample. The image can be observed directly by eyes through an ocular lens, recorded by a light-sensitive plate or a digital image detector such as CCD or CMOS, and displayed and analyzed on a computer. Of course, by combining with the camera device, it is also possible to record a video or the like about the specimen in the field of view. However, the field of view that can be observed by the microscope is limited, and when the size of the sample to be observed exceeds the current field of view, only the condition of the sample in the current field of view can be observed at the same time.

Disclosure of Invention

It is an object of the present application to provide a method and apparatus for acquiring microscopic images.

According to one aspect of the application, a method for acquiring a microscopic image is provided, which is applied to a computing device, and the computing device establishes a communication connection with a corresponding microscopic device, wherein the method comprises the following steps:

acquiring current fluorescence parameter information currently used by the microscopic equipment, wherein the fluorescence parameter information is used for determining the corresponding current excitation light wavelength;

sending an image acquisition instruction to the microscopic equipment;

and receiving a microscopic image sequence which is shot by the microscopic equipment based on the image acquisition instruction and is about to the sample so as to acquire a corresponding microscopic image, wherein the microscopic image sequence comprises microscopic sub-images respectively corresponding to one or more sub-areas of the sample, the microscopic sub-images are used for splicing to obtain the microscopic image, and the microscopic sub-images correspond to the current excitation light wavelength.

According to another aspect of the present application, there is provided a method for acquiring a microscopic image, applied to a microscopic device that establishes a communication connection with a corresponding computing device, wherein the method comprises:

receiving an image acquisition instruction sent by the computing equipment;

acquiring a microscopic image sequence related to the sample based on the image acquisition instruction, wherein the microscopic image sequence comprises microscopic sub-images respectively corresponding to one or more sub-areas of the sample, and the microscopic sub-images are used for splicing to obtain microscopic images corresponding to the sample;

sending the sequence of microscopic images to the computing device.

According to one aspect of the present application, there is provided a method for acquiring a microscopic image, the method comprising:

the computing equipment acquires current fluorescence parameter information currently used by corresponding microscopic equipment, wherein the computing equipment is in communication connection with the microscopic equipment, and the fluorescence parameter information is used for determining the corresponding current excitation light wavelength;

the computing equipment sends an image acquisition instruction to the microscopic equipment;

the microscopic equipment receives an image acquisition instruction sent by the computing equipment;

the microscopic equipment acquires a microscopic image sequence related to the sample based on the image acquisition instruction, wherein the microscopic image sequence comprises microscopic sub-images respectively corresponding to one or more sub-areas of the sample, and the microscopic sub-images are used for splicing to obtain microscopic images corresponding to the sample;

the microscopic device sends the microscopic image sequence to the computing device;

the computing device receives a sequence of microscopic images of the specimen taken by the microscopic device based on the image acquisition instructions to acquire corresponding microscopic images.

According to an aspect of the present application, there is provided a computing device for acquiring microscopic images, the device comprising:

the one-to-one module is used for acquiring current fluorescence parameter information currently used by the microscopic equipment, wherein the fluorescence parameter information is used for determining the corresponding current excitation light wavelength;

a second module, configured to send an image acquisition instruction to the microscopic device;

and the three modules are used for receiving a microscopic image sequence which is shot by the microscopic equipment based on the image acquisition instruction and is about to the sample so as to acquire corresponding microscopic images, wherein the microscopic image sequence comprises one or more microscopic sub-images respectively corresponding to one or more sub-regions of the sample, the microscopic sub-images are used for splicing to obtain the microscopic images, and the microscopic sub-images correspond to the current excitation light wavelength.

According to another aspect of the present application, there is provided a microscopy apparatus for acquiring a microscopy image, the apparatus comprising:

the first module is used for receiving an image acquisition instruction sent by the computing equipment;

a second module, configured to acquire a microscopic image sequence related to the sample based on the image acquisition instruction, where the microscopic image sequence includes microscopic sub-images corresponding to one or more sub-regions of the sample, and the microscopic sub-images are used to obtain microscopic images corresponding to the sample by stitching;

and a second module and a third module for sending the microscopic image sequence to the computing device.

According to an aspect of the present application, there is provided an apparatus for acquiring microscopic images, wherein the apparatus comprises:

a processor; and

a memory arranged to store computer executable instructions that, when executed, cause the processor to perform the operations of any of the methods described above.

According to another aspect of the application, there is provided a computer readable medium storing instructions that, when executed, cause a system to perform the operations of any of the methods described above.

Compared with the prior art, the method and the device have the advantages that the current fluorescence parameter information currently used by the microscopic equipment is obtained, wherein the fluorescence parameter information is used for determining the corresponding current excitation light wavelength; sending an image acquisition instruction to the microscopic equipment; and receiving a microscopic image sequence which is shot by the microscopic equipment based on the image acquisition instruction and is about to the sample so as to acquire a corresponding microscopic image, wherein the microscopic image sequence comprises microscopic sub-images respectively corresponding to one or more sub-areas of the sample, the microscopic sub-images are used for splicing to obtain the microscopic image, and the microscopic sub-images correspond to the current excitation light wavelength. According to the method and the device, the microscopic image sequences of the sample under different fluorescence parameter information can be obtained, and the microscopic images of all the collectable ranges of the sample are generated based on the microscopic image sequences, so that more detailed, concrete and complete images or research data can be obtained, and good use experience is provided for users.

Drawings

Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings in which:

FIG. 1 shows a flow diagram of a method for acquiring microscopic images according to one embodiment of the present application;

FIG. 2 shows a flow diagram of a method for acquiring microscopic images according to another embodiment of the present application;

FIG. 3 illustrates a device structure diagram of computing device 100, according to one embodiment of the present application;

FIG. 4 shows an apparatus block diagram of a microscopy apparatus 100 according to an embodiment of the present application;

FIG. 5 illustrates an exemplary system that can be used to implement the various embodiments described in this application.

The same or similar reference numbers in the drawings identify the same or similar elements.

Detailed Description

The present application is described in further detail below with reference to the attached figures.

In a typical configuration of the present application, the terminal, the device serving the network, and the trusted party each include one or more processors (e.g., Central Processing Units (CPUs)), input/output interfaces, network interfaces, and memory.

The Memory may include forms of volatile Memory, Random Access Memory (RAM), and/or non-volatile Memory in a computer-readable medium, such as Read Only Memory (ROM) or Flash Memory. Memory is an example of a computer-readable medium.

Computer-readable media, including both non-transitory and non-transitory, removable and non-removable media, may implement information storage by any method or technology. The information may be computer readable instructions, data structures, modules of a program, or other data. Examples of computer storage media include, but are not limited to, Phase-Change Memory (PCM), Programmable Random Access Memory (PRAM), Static Random-Access Memory (SRAM), Dynamic Random Access Memory (DRAM), other types of Random Access Memory (RAM), Read Only Memory (ROM), electrically Erasable Programmable Read-Only Memory (EEPROM), flash Memory or other Memory technology, Compact Disc Read-Only Memory (CD-ROM), Digital Versatile Discs (DVD) or other optical storage, magnetic cassettes, magnetic tape, magnetic disk storage or other magnetic storage devices, or any other non-transmission medium, which can be used to store information that can be accessed by a computing device.

The device referred to in this application includes, but is not limited to, a user device, a network device, or a device formed by integrating a user device and a network device through a network. The user equipment includes, but is not limited to, any mobile electronic product, such as a smart phone, a tablet computer, etc., capable of performing human-computer interaction with a user (e.g., human-computer interaction through a touch panel), and the mobile electronic product may employ any operating system, such as an Android operating system, an iOS operating system, etc. The network Device includes an electronic Device capable of automatically performing numerical calculation and information processing according to a preset or stored instruction, and the hardware includes, but is not limited to, a microprocessor, an Application Specific Integrated Circuit (ASIC), a Programmable Logic Device (PLD), a Field Programmable Gate Array (FPGA), a Digital Signal Processor (DSP), an embedded Device, and the like. The network device includes but is not limited to a computer, a network host, a single network server, a plurality of network server sets or a cloud of a plurality of servers; here, the Cloud is composed of a large number of computers or web servers based on Cloud Computing (Cloud Computing), which is a kind of distributed Computing, one virtual supercomputer consisting of a collection of loosely coupled computers. Including, but not limited to, the internet, a wide area network, a metropolitan area network, a local area network, a VPN network, a wireless Ad Hoc network (Ad Hoc network), etc. Preferably, the device may also be a program running on the user device, the network device, or a device formed by integrating the user device and the network device, the touch terminal, or the network device and the touch terminal through a network.

Of course, those skilled in the art will appreciate that the foregoing is by way of example only, and that other existing or future devices, which may be suitable for use in the present application, are also encompassed within the scope of the present application and are hereby incorporated by reference.

In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

Fig. 1 shows a method for acquiring microscopic images according to an aspect of the present application, the method being applied to a computing device 100, the computing device 100 establishing a communication connection with a corresponding microscopic device 200, the method comprising steps S101, S102 and S103. In step S101, a computing device obtains current fluorescence parameter information currently used by the microscopy device, where the fluorescence parameter information is used to determine a corresponding current excitation light wavelength; in step S102, the computing device sends an image acquisition instruction to the microscopic device; in step S103, the computing device receives a microscopic image sequence about the sample, which is taken by the microscopic device based on the image acquisition instruction, to acquire corresponding microscopic images, where the microscopic image sequence includes microscopic sub-images respectively corresponding to one or more sub-regions of the sample, and the microscopic sub-images are used for obtaining the microscopic images by stitching, and the microscopic sub-images correspond to the current excitation light wavelength. Herein, the computing device includes, but is not limited to, a user device, a network device, or a device formed by integrating the user device and the network device through a network, the user device includes, but is not limited to, any terminal capable of performing human-computer interaction with a user (e.g., human-computer interaction through a touch panel), and the network device includes, but is not limited to, a computer, a network host, a single network server, a plurality of network server sets, or a cloud formed by a plurality of servers. The computing equipment comprises a communication device, a display device and a control device, wherein the communication device is used for establishing communication connection with corresponding microscopic equipment so as to send related instructions to the microscopic equipment and receive related images and the like acquired by the microscopic equipment; the computing device also includes a data processing apparatus for storing, processing, etc. the received data. The microscopic equipment comprises a working component, an objective table, a fluorescent device and the like, wherein the fluorescent device comprises a falling light device, an excitation filter plate, a pressing filter plate and the like, the falling light device is used as a light source to emit corresponding initial light, the initial light passes through the excitation filter plate to obtain excitation light for exciting the fluorescent effect of a sample, the sample forms emission light through the pressing filter plate through fluorescent light obtained by the fluorescent effect, so that a corresponding fluorescent image is presented on the image, wherein the excitation filter plate or the pressing filter plate is used for filtering light of a certain waveband to enable the light of a specific waveband to pass through, the microscopic equipment comprises but is not limited to a high-pass filter plate, a low-pass filter plate, a band-pass filter plate or a line-pass filter plate and the like, and the excitation filter plate and the pressing filter plate work cooperatively to obtain a good fluorescent image about the sample. The working assembly includes an objective lens assembly or other working assembly for performing an operation, and the like, and the objective lens and the stage are kept in relative motion during image acquisition so that the objective lens images different areas of the sample, and in some embodiments, for example, the stage moves in a planar direction, and the objective lens moves in a vertical direction of the planar direction, and the moving components providing corresponding driving force include, but are not limited to, a stepping motor and a lead screw structure, and the like. Based on the application, different researchers can reproduce the change of the sample along with the wavelength of the excitation light based on the obtained microscopic image without carrying out complicated related tests.

Specifically, in step S101, the computing device acquires current fluorescence parameter information currently being used by the microscopy device, wherein the fluorescence parameter information is used for determining a corresponding current excitation light wavelength. For example, the fluorescence parameter information includes, but is not limited to, the initial light wavelength and excitation filter plate corresponding to the falling light device, and in some embodiments, the fluorescence parameter information further includes a pressing filter plate; the excitation light wavelength for exciting the fluorescence effect of the sample can be determined based on the initial light wavelength and the excitation filter plate, wherein the excitation light wavelength can be a waveband range or a wavelength value, if the waveband range is the waveband range, the excitation light wavelength is represented by a central value and a corresponding neighborhood of the waveband range, and the corresponding neighborhood is a wavelength difference value from the central value wavelength to a range boundary wavelength. The computing device may obtain current fluorescence parameter information currently used by the microscopy device, such as receiving the current fluorescence parameter information sent by the microscopy device, or sending a corresponding control instruction to the microscopy device to adjust the current fluorescence parameter information of the microscopy device. As in some embodiments, in step S101, a computing device sends fluorescence parameter acquisition instructions to the microscopy device; and receiving current fluorescence parameter information returned by the microscopic equipment based on the fluorescence parameter acquisition instruction, wherein the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength. For example, the computing device establishes a communication connection with the corresponding microscopy apparatus, based on which the computing device may send a fluorescence parameter acquisition instruction to the microscopy apparatus, the fluorescence parameter acquisition instruction being used to request corresponding current fluorescence parameter information from the microscopy apparatus, based on which the computing device may determine a current excitation light wavelength or the like in the current fluorescence device, such as an excitation light wavelength from the initial light wavelength and the excitation filter plate or the like. In other embodiments, in step S101, the computing device generates a corresponding fluorescence parameter control instruction based on an input operation of a user, wherein the fluorescence parameter control instruction is used to adjust current fluorescence parameter information of the microscopy device, wherein the current fluorescence parameter information is used to determine a corresponding current excitation light wavelength. For example, the computing device may send a control instruction to the microscopy device based on the communication connection with the microscopy device, the control instruction being used to adjust the fluorescence apparatus of the microscopy device to adjust the corresponding current fluorescence parameter information; the corresponding current fluorescence parameter information may be input by a user in the computing device, for example, by inputting a corresponding numerical value or selecting a corresponding filter plate or initial optical band, etc., to determine a corresponding fluorescence parameter control instruction, the microscopic device receives the fluorescence parameter control instruction and applies the corresponding control instruction to the fluorescence parameter device, and adjusts the corresponding initial optical band or excitation filter plate, etc., to ensure that the corresponding current excitation optical wavelength is consistent with the current excitation optical wave required by the computing device, etc.

In step S102, the computing device sends an image acquisition instruction to the microscopic device. For example, the computing device sends to the microscopy device corresponding image acquisition instructions for requesting from the microscopy device acquisition image data corresponding to the current fluorescence parameter information, which in some embodiments comprises a sequence of microscopy images of microscopy sub-images corresponding to one or more sub-regions of the sample. Here, the execution of step S101 may be performed before step S102 or simultaneously with step S102, or the like.

In step S103, the computing device receives a microscopic image sequence about the sample, which is taken by the microscopic device based on the image acquisition instruction, to acquire corresponding microscopic images, where the microscopic image sequence includes microscopic sub-images respectively corresponding to one or more sub-regions of the sample, and the microscopic sub-images are used for obtaining the microscopic images by stitching, and the microscopic sub-images correspond to the current excitation light wavelength. For example, the microscopic image sequence includes microscopic sub-image information of one or more sub-regions of the sample based on the current excitation light wavelength, where the microscopic sub-image information of each sub-region includes a scanned image of the sub-region of the sample, and for example, the microscopic sub-image information includes acquired image information acquired based on the sample at different focal plane heights, or a plurality of acquired image information acquired at the same focal plane height, and the like, a corresponding sub-region image of the sub-region may be obtained based on the plurality of microscopic sub-images of the sub-region, such as a microscopic sub-region image of the sub-region formed by depth-of-field fusion, where depth-field fusion is used to take pixels corresponding to depths of different acquired image information, and perform stitching to obtain an image with higher definition in the whole range, and the like. The sequence of microscope images comprises information of microscope sub-images of one or more sub-areas, the sequence of microscope images being used to assemble a microscope image relating to the sample, the microscope image comprising all or part of the collectable area of the sample, etc., e.g. a user specifies a target area for collecting the sample, the target area comprising only part of the collectable area of the sample, the computing device may generate the corresponding microscope image based on the sequence of one or more microscope images comprised by the collected image data. The generated microscopic image may be a two-dimensional microscopic image or a three-dimensional microscopic image, where the three-dimensional microscopic image includes three-dimensional coordinate information of an overall contour of the sample, for example, a corresponding three-dimensional image coordinate system is established in the three-dimensional microscopic image, each point on the contour corresponding to the sample has a corresponding three-dimensional coordinate in the three-dimensional image coordinate system, and the three-dimensional microscopic image may present three-dimensional contours of different angles of the sample through a specific plug-in or application, etc. The microscopic image sequence corresponds to the wavelength of the excitation light during acquisition, and if the corresponding microscopic image is only generated by the microscopic image sequence or the corresponding excitation light wavelengths of the microscopic sub-images for generating the microscopic image are the same, the same excitation light wavelength is used as the excitation light wavelength corresponding to the microscopic image; if the excitation light wavelengths corresponding to the microscopic image sequences for generating the microscopic images are different, marking the microscopic images as the microscopic images generated by different excitation light wavelengths, and taking the different excitation light wavelengths as the corresponding comprehensive excitation light wavelengths, for example, each area in the images respectively corresponds to the different excitation light wavelengths. In some embodiments, the acquisition region of the specimen includes a plurality of sub-regions, and in step S103, a sequence of microscope images of the specimen, which are taken by the microscope device based on the image acquisition instruction, is received, wherein the sequence of microscope images includes images of the plurality of sub-regions of the specimen, each image of a sub-region includes microscope sub-images corresponding to a plurality of focal plane heights of the sub-region, respectively, and the microscope sub-images correspond to the current excitation light wavelength; determining three-dimensional coordinate information corresponding to each pixel in each sub-area based on a plurality of microscopic sub-images corresponding to each sub-image; stitching the images of the plurality of sub-regions to obtain a microscope image corresponding to the current excitation light wavelength. For example, the collection area of the specimen comprises a plurality of sub-areas, wherein each sub-area corresponds to the area of the microscope field of view size, the collection area of the target object formed by combining the plurality of sub-areas comprises all or part of all the collectable areas of the specimen, and the microscope field of view size is inversely proportional to the multiplying power of the objective lens. The method comprises the steps that a computing device obtains a microscopic image sequence corresponding to at least one sub-area selected by a user in a plurality of sub-areas, wherein the microscopic image sequence comprises microscopic sub-images formed by different focal plane heights of the sub-areas, and based on the microscopic sub-images, the computing device can generate images of the sub-areas corresponding to the sub-areas, such as three-dimensional images of the sub-areas and the like, wherein the microscopic sub-images correspond to the current excitation light wavelength, the three-dimensional image information of the sub-areas comprises a corresponding three-dimensional image coordinate system, and corresponding three-dimensional coordinate information exists in the image coordinate system of each pixel in the images; and then, the computing equipment splices the three-dimensional microscopic sub-image information of the plurality of sub-areas to obtain a microscopic image corresponding to the wavelength of the current excitation light.

In some embodiments, the method further comprises step S104 (not shown). In step S104, the computing device stores respective microscopic images for the specimen, wherein each microscopic image corresponds to the current excitation light wavelength acquired when the microscopic image was acquired. For example, after acquiring a microscopic image of the sample, the computing device maps the microscopic image with the current excitation light wavelength acquired when the microscopic image is acquired, and stores the microscopic image in the computing device for generating a corresponding microscopic video. As in some embodiments, the method further comprises step S105 (not shown). In step S105, the computing device generates an excitation light sequence in order based on each current excitation light wavelength; and displaying the microscopic images corresponding to the excitation light wavelengths based on the excitation light sequence according to a certain time interval. For example, the computing device sorts the excitation light wavelengths of the microscope images in a sequence (e.g., from short to long or from long to short) according to the current excitation light wavelengths to form a corresponding excitation light sequence, then arranges the microscope images according to the excitation light sequence, and presents the microscope images at certain time intervals, wherein the presentation sequence of the microscope images is the same as the arrangement sequence of the excitation light sequence. Wherein the time interval may be a user setting or a setting based on default parameters (e.g., twenty frames per second, etc.), etc.

In some embodiments, the method further comprises step S106 (not shown). In step S106, the computing device transmits, to the corresponding network device, each of the microscopic images about the specimen, each of which corresponds to the current excitation light wavelength acquired when the microscopic image was acquired. For example, the computing device establishes communication connection with the corresponding network device, and sends the corresponding microscopic images to the network device through the communication connection with the network device, wherein the network device receives and stores the microscopic images, each microscopic image has corresponding excitation light wavelength, and the network device associates the microscopic images with identification information of the sample, such as the name and unique code of the sample, when storing, so that a subsequent user can conveniently obtain the related microscopic images through the corresponding application of the network device.

In some embodiments, the collection area of the specimen comprises a plurality of sub-areas, wherein the method further comprises step S107 (not shown). In step S107, the computing device receives a plurality of microscopic image sequences with respect to the specimen, which are taken by the microscopic device based on the image acquisition instruction, wherein each microscopic image sequence includes an image of at least one region of the plurality of sub-regions of the specimen, and each microscopic image sequence corresponds to a current excitation light wavelength acquired when the microscopic image sequence is acquired; stitching a microscopic image for the specimen based on the plurality of sequences of microscopic images. For example, the acquisition region of the sample includes a plurality of sub-regions, the plurality of sub-regions are all or part of the complete acquirable region of the sample, the computing device controls the microscopy device to acquire at least one sub-region in each sub-region each time, so as to acquire a microscope sub-image corresponding to each sub-region, based on the difference of the wavelength of the excitation light at each acquisition, the wavelength of the excitation light corresponding to the microscope sub-image of each acquired sub-region is different, so that the acquired microscope image includes a plurality of image regions, each image region includes at least one sub-region, the wavelength of the excitation light of each image region is not completely the same, and the like. If the computing equipment sends an image acquisition instruction to the microscopic equipment, the image acquisition instruction comprises an acquisition area corresponding to the acquired image, the acquisition area comprises at least one subarea in the plurality of subareas and the like, the microscopic equipment moves the objective lens to the corresponding acquisition area based on the image acquisition instruction, and acquires the corresponding microscopic sub-image based on the current fluorescence parameter information. In some embodiments, said stitching the microscopic images for the specimen based on the plurality of sequences of microscopic images comprises: generating images corresponding to the plurality of sub-regions corresponding to each microscopic image sequence according to the image corresponding to each microscopic image sequence, wherein the image corresponding to each sub-region has an excitation light wavelength corresponding to the excitation light wavelength; stitching a microscopic image about the specimen from the images of the plurality of sub-regions. For example, an image of each sub-region is generated according to the microscopic image sequence acquired each time, such as an image of the corresponding sub-region is determined according to a plurality of microscopic sub-images through depth-of-field fusion, direct optimization or based on three-dimensional coordinates of pixels, wherein the image of the sub-region can be a two-dimensional image or a three-dimensional image, and a current excitation light wavelength of each sub-region in the microscopic sub-image corresponds to the image; subsequently, the network device stitches the microscopic images of the acquisition area of the sample based on the microscopic sub-images of the plurality of sub-areas, such as by an image stitching technique or the like.

In some embodiments, in step S102, if the current excitation light wavelength satisfies the image acquisition condition, an image acquisition instruction is sent to the microscopy apparatus. For example, an image acquisition judgment condition is preset in the computing device, the judgment is performed based on the current excitation light wavelength, if the image acquisition condition is met, the computing device sends an image acquisition instruction to the microscopy device, and if the image acquisition condition is not met, the computing device can continuously regulate and control the corresponding microscopy device or wait for the current excitation light wavelength corresponding to the current fluorescence parameter information in the microscopy device to meet the image acquisition condition. As in some embodiments, the image acquisition conditions include, but are not limited to: the current excitation light wavelength exists in a set with a target excitation light wavelength; and the wavelength difference between the current excitation light wavelength and the preamble excitation light wavelength corresponding to the preamble microscopic image reaches a preset wavelength difference. For example, the computing device establishes a corresponding target excitation light wavelength set for adjusting current fluorescence parameter information of the microscopy device and the like based on user setting, default setting and the like, and if the current excitation light wavelength exists in the target excitation light wavelength set, the current excitation light wavelength meets image acquisition conditions and the like. For another example, each acquisition is set to a certain difference, and if the wavelength difference between the current excitation light wavelength and the preamble excitation light wavelength of the preamble microscopic image reaches a preset wavelength difference, it is determined that the current excitation light wavelength satisfies the image acquisition condition, etc., it should be understood by those skilled in the art that the above-mentioned image acquisition condition is merely an example, and other existing or future image acquisition conditions may be included in the scope of the present application, and are included herein by reference. In some embodiments, the image acquisition condition includes that a wavelength difference between the current excitation light wavelength and a preamble excitation light wavelength corresponding to a preamble microscopic image reaches a preset wavelength difference, wherein the method further includes step S108 (not shown), and in step S108, if the preamble microscopic image is received, a fluorescence parameter control instruction is sent to the microscopic apparatus, where the fluorescence parameter control instruction is used to adjust current fluorescence parameter information of the microscopic apparatus. For example, after the computing device obtains a microscopic image sequence acquired by the microscopic device through an image acquisition instruction each time, a corresponding fluorescence parameter control instruction is generated based on the microscopic image sequence acquired in the preamble (the last time of the current acquisition, and the current time of the current acquisition), the fluorescence parameter control instruction is used for adjusting fluorescence parameter information of the microscopic device, and the current fluorescence parameter information corresponding to the current acquisition is adjusted from the previous fluorescence parameter information, for example, the wavelength of the corresponding preamble excitation light is decreased or a preset wavelength difference is added according to a specific sequence. Based on the adjusted current fluorescence parameter information, the computing equipment sends an image acquisition instruction to the microscopic equipment to acquire a microscopic image sequence corresponding to the acquisition.

Fig. 2 shows a method for acquiring a microscope image according to another aspect of the present application, which is applied to a microscope device that establishes a communication connection with a corresponding computing device, wherein the method comprises step S201, step S202 and step S203. In step S201, a microscopic device receives an image acquisition instruction sent by the computing device; in step S202, a microscope device acquires a sequence of microscope images about a sample based on the image acquisition instruction, where the sequence of microscope images includes microscope sub-images corresponding to one or more sub-regions of the sample, and the microscope sub-images are used for stitching to obtain microscope images corresponding to the sample; in step S203, the microscopic device sends the sequence of microscopic images to the computing device. For example, the fluorescence parameter information includes, but is not limited to, the initial light wavelength and excitation filter plate corresponding to the falling light device, and in some embodiments, the fluorescence parameter information further includes a pressing filter plate; the excitation light wavelength for exciting the fluorescence effect of the sample can be determined based on the initial light wavelength and the excitation filter plate, wherein the excitation light wavelength can be a waveband range or a wavelength value, if the waveband range is the waveband range, the excitation light wavelength is represented by a central value and a corresponding neighborhood of the waveband range, and the corresponding neighborhood is a wavelength difference value from the central value wavelength to a range boundary wavelength. The computing device may obtain current fluorescence parameter information currently used by the microscopy device, such as receiving the current fluorescence parameter information sent by the microscopy device, or sending a corresponding control instruction to the microscopy device to adjust the current fluorescence parameter information of the microscopy device. The computing device sends to the microscopy device a corresponding image acquisition instruction for requesting to the microscopy device a sequence of acquired image data comprising microscopic sub-images corresponding to one or more sub-regions of the sample, in some embodiments, to the current fluorescence parameter information. The microscopic image sequence includes microscopic sub-image information of one or more sub-regions of the sample based on the current excitation light wavelength, where the microscopic sub-image information of each sub-region includes a scanned image of the sub-region of the sample, and for example, the microscopic sub-image information includes acquired image information acquired based on the sample at different focal plane heights, or multiple acquired image information acquired at the same focal plane height, and the like. And then, after acquiring the corresponding microscopic image sequence, the microscopic device sends the microscopic image sequence to the computing device.

In some embodiments, the method further comprises step S204 (not shown). In step S204, the microscopic device receives the fluorescence parameter acquisition instruction sent by the computing device, and returns current fluorescence parameter information to the user equipment based on the fluorescence parameter acquisition instruction, where the current fluorescence parameter information is used to determine a corresponding current excitation light wavelength. For example, the computing device establishes a communication connection with the corresponding microscopy apparatus, based on which the computing device may send a fluorescence parameter acquisition instruction to the microscopy apparatus, the fluorescence parameter acquisition instruction being used to request corresponding current fluorescence parameter information from the microscopy apparatus, based on which the computing device may determine a current excitation light wavelength or the like in the current fluorescence device, such as an excitation light wavelength from the initial light wavelength and the excitation filter plate or the like. In some embodiments, the method further comprises step S205 (not shown). In step S205, the microscopy device receives the fluorescence parameter control instruction sent by the computing device, and adjusts current fluorescence parameter information of the microscopy device based on the fluorescence parameter control instruction, where the current fluorescence parameter information is used to determine a corresponding current excitation light wavelength. For example, the computing device may send a control instruction to the microscopy device based on the communication connection with the microscopy device, the control instruction being used to adjust the fluorescence apparatus of the microscopy device to adjust the corresponding current fluorescence parameter information; the corresponding current fluorescence parameter information may be input by a user in the computing device, for example, by inputting a corresponding numerical value or selecting a corresponding filter plate or initial optical band, etc., to determine a corresponding fluorescence parameter control instruction, the microscopic device receives the fluorescence parameter control instruction and applies the corresponding control instruction to the fluorescence parameter device, and adjusts the corresponding initial optical band or excitation filter plate, etc., to ensure that the corresponding current excitation optical wavelength is consistent with the current excitation optical wave required by the computing device, etc.

In some embodiments, in step S201, a microscopic apparatus receives an image acquisition instruction sent by the computing apparatus, wherein the image acquisition instruction includes at least one sub-region of a plurality of sub-regions of the specimen; in step S202, the microscope device acquires a sequence of microscope images about the sample based on the image acquisition instruction, where the sequence of microscope images includes microscope sub-images corresponding to the at least one sub-region, and the microscope sub-images are used for stitching to obtain microscope images corresponding to the sample. For example, the acquisition region of the sample includes a plurality of sub-regions, the plurality of sub-regions are all or part of the complete acquirable region of the sample, the computing device controls the microscopy device to acquire at least one sub-region in each sub-region each time, so as to acquire a microscope sub-image corresponding to each sub-region, based on the difference of the wavelength of the excitation light at each acquisition, the wavelength of the excitation light corresponding to the microscope sub-image of each acquired sub-region is different, so that the acquired microscope image includes a plurality of image regions, each image region includes at least one sub-region, the wavelength of the excitation light of each image region is not completely the same, and the like. If the computing equipment sends an image acquisition instruction to the microscopic equipment, the image acquisition instruction comprises an acquisition area corresponding to the acquired image, the acquisition area comprises at least one subarea in the plurality of subareas and the like, the microscopic equipment moves the objective lens to the corresponding acquisition area based on the image acquisition instruction, and acquires the corresponding microscopic sub-image based on the current fluorescence parameter information.

According to one aspect of the present application, there is provided a method for acquiring a microscopic image, wherein the method comprises:

the computing equipment acquires current fluorescence parameter information currently used by corresponding microscopic equipment, wherein the computing equipment is in communication connection with the microscopic equipment, and the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength;

Fig. 3 shows a computing device 100 for acquiring microscopic images according to an aspect of the present application, the computing device 100 establishing a communication connection with a corresponding microscopic device 200, the device comprising a one-module 101, a two-module 102, and a three-module 103. A module 101, configured to obtain current fluorescence parameter information currently used by the microscopy apparatus, where the fluorescence parameter information is used to determine a corresponding current excitation light wavelength; a module 102, configured to send an image acquisition instruction to the microscopic device; a module 103, configured to receive a microscopic image sequence about the sample, which is shot by the microscopic apparatus based on the image acquisition instruction, so as to acquire a corresponding microscopic image, where the microscopic image sequence includes microscopic sub-images corresponding to one or more sub-regions of the sample, respectively, and the microscopic sub-images are used for obtaining the microscopic image by stitching, where the microscopic sub-images correspond to the current excitation light wavelength. Here, the specific implementation corresponding to the one-to-one module 101, the two-to-two module 102, and the one-to-three module 103 shown in fig. 3 is the same as or similar to the embodiment of the step S101, the step S102, and the step S103 shown in fig. 1, and therefore, the detailed description is omitted, and the specific implementation is included herein by reference.

In some embodiments, the apparatus further comprises a four module (not shown), a five module (not shown), a six module (not shown), a seven module (not shown), and an eight module (not shown). And the four modules are used for storing various microscopic images of the sample, wherein each microscopic image corresponds to the current excitation light wavelength acquired when the microscopic image is acquired. The module of one five, is used for producing the exciting light sequence according to the order on the basis of every present exciting light wavelength; and displaying the microscopic images corresponding to the excitation light wavelengths based on the excitation light sequence according to a certain time interval. And the six modules are used for sending each microscopic image about the sample to corresponding network equipment, wherein each microscopic image corresponds to the current excitation light wavelength acquired when the microscopic image is acquired. A seventh module for receiving a plurality of microscopic image sequences about the specimen taken by the microscopic device based on the image acquisition instructions, wherein each microscopic image sequence comprises an image of at least one of the plurality of sub-regions of the specimen, and each microscopic image sequence corresponds to a current excitation light wavelength acquired when the microscopic image sequence is acquired; stitching a microscopic image for the specimen based on the plurality of sequences of microscopic images. And the eight module is used for sending a fluorescence parameter control instruction to the microscopic equipment if the preorder microscopic image is received, wherein the fluorescence parameter control instruction is used for adjusting the current fluorescence parameter information of the microscopic equipment. Here, the specific implementation corresponding to the four to eight modules is the same as or similar to the embodiment of the steps S104 to S108, and thus is not repeated herein and is included by reference.

Fig. 4 shows a microscopy apparatus 200 for acquiring a microscopy image according to another aspect of the present application, the microscopy apparatus establishing a communication connection with a corresponding computing apparatus, wherein the apparatus comprises a two-in-one module 201, a two-in-two module 202 and a two-in-three module 203. A second-first module 201, configured to receive an image acquisition instruction sent by the computing device; a second module 202, configured to acquire a microscopic image sequence related to the sample based on the image acquisition instruction, where the microscopic image sequence includes microscopic sub-images corresponding to one or more sub-regions of the sample, and the microscopic sub-images are used to obtain microscopic images corresponding to the sample by stitching; a second and third module 203 for sending the sequence of microscopic images to the computing device. Here, the specific implementation corresponding to the two-to-one module 201, the two-to-two module 202, and the two-to-three module 203 shown in fig. 4 is the same as or similar to the embodiment of the step S201, the step S202, and the step S203 shown in fig. 2, and therefore, the detailed description is not repeated and is included herein by reference.

In some embodiments, the apparatus further comprises two four modules (not shown), two five modules (not shown). The second module and the fourth module are configured to receive a fluorescence parameter acquisition instruction sent by the computing device, and return current fluorescence parameter information to the user equipment based on the fluorescence parameter acquisition instruction, where the current fluorescence parameter information is used to determine a corresponding current excitation light wavelength. And the second-fifth module is used for receiving a fluorescence parameter control instruction sent by the computing equipment and adjusting the current fluorescence parameter information of the microscopic equipment based on the fluorescence parameter control instruction, wherein the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength. Here, the specific implementation corresponding to the two-four to two-five modules is the same as or similar to the embodiment of the foregoing steps S204 to S208, and thus is not repeated here, and is included herein by way of reference.

In addition to the methods and apparatus described in the embodiments above, the present application also provides a computer readable storage medium storing computer code that, when executed, performs the method as described in any of the preceding claims.

The present application also provides a computer program product, which when executed by a computer device, performs the method of any of the preceding claims.

The present application further provides a computer device, comprising:

one or more processors;

a memory for storing one or more computer programs;

the one or more computer programs, when executed by the one or more processors, cause the one or more processors to implement the method of any preceding claim.

FIG. 5 illustrates an exemplary system that can be used to implement the various embodiments described herein;

in some embodiments, as shown in FIG. 5, the system 300 can be implemented as any of the above-described devices in the various embodiments. In some embodiments, system 300 may include one or more computer-readable media (e.g., system memory or NVM/storage 320) having instructions and one or more processors (e.g., processor(s) 305) coupled with the one or more computer-readable media and configured to execute the instructions to implement modules to perform the actions described herein.

For one embodiment, system control module 310 may include any suitable interface controllers to provide any suitable interface to at least one of processor(s) 305 and/or any suitable device or component in communication with system control module 310.

The system control module 310 may include a memory controller module 330 to provide an interface to the system memory 315. Memory controller module 330 may be a hardware module, a software module, and/or a firmware module.

System memory 315 may be used, for example, to load and store data and/or instructions for system 300. For one embodiment, system memory 315 may include any suitable volatile memory, such as suitable DRAM. In some embodiments, the system memory 315 may include a double data rate type four synchronous dynamic random access memory (DDR4 SDRAM).

For one embodiment, system control module 310 may include one or more input/output (I/O) controllers to provide an interface to NVM/storage 320 and communication interface(s) 325.

For example, NVM/storage 320 may be used to store data and/or instructions. NVM/storage 320 may include any suitable non-volatile memory (e.g., flash memory) and/or may include any suitable non-volatile storage device(s) (e.g., one or more Hard Disk Drives (HDDs), one or more Compact Disc (CD) drives, and/or one or more Digital Versatile Disc (DVD) drives).

NVM/storage 320 may include storage resources that are physically part of the device on which system 300 is installed or may be accessed by the device and not necessarily part of the device. For example, NVM/storage 320 may be accessible over a network via communication interface(s) 325.

Communication interface(s) 325 may provide an interface for system 300 to communicate over one or more networks and/or with any other suitable device. System 300 may wirelessly communicate with one or more components of a wireless network according to any of one or more wireless network standards and/or protocols.

For one embodiment, at least one of the processor(s) 305 may be packaged together with logic for one or more controller(s) (e.g., memory controller module 330) of the system control module 310. For one embodiment, at least one of the processor(s) 305 may be packaged together with logic for one or more controller(s) of the system control module 310 to form a System In Package (SiP). For one embodiment, at least one of the processor(s) 305 may be integrated on the same die with logic for one or more controller(s) of the system control module 310. For one embodiment, at least one of the processor(s) 305 may be integrated on the same die with logic for one or more controller(s) of the system control module 310 to form a system on a chip (SoC).

In various embodiments, system 300 may be, but is not limited to being: a server, a workstation, a desktop computing device, or a mobile computing device (e.g., a laptop computing device, a handheld computing device, a tablet, a netbook, etc.). In various embodiments, system 300 may have more or fewer components and/or different architectures. For example, in some embodiments, system 300 includes one or more cameras, a keyboard, a Liquid Crystal Display (LCD) screen (including a touch screen display), a non-volatile memory port, multiple antennas, a graphics chip, an Application Specific Integrated Circuit (ASIC), and speakers.

It should be noted that the present application may be implemented in software and/or a combination of software and hardware, for example, implemented using Application Specific Integrated Circuits (ASICs), general purpose computers or any other similar hardware devices. In one embodiment, the software programs of the present application may be executed by a processor to implement the steps or functions described above. Likewise, the software programs (including associated data structures) of the present application may be stored in a computer readable recording medium, such as RAM memory, magnetic or optical drive or diskette and the like. Additionally, some of the steps or functions of the present application may be implemented in hardware, for example, as circuitry that cooperates with the processor to perform various steps or functions.

In addition, some of the present application may be implemented as a computer program product, such as computer program instructions, which when executed by a computer, may invoke or provide methods and/or techniques in accordance with the present application through the operation of the computer. Those skilled in the art will appreciate that the form in which the computer program instructions reside on a computer-readable medium includes, but is not limited to, source files, executable files, installation package files, and the like, and that the manner in which the computer program instructions are executed by a computer includes, but is not limited to: the computer directly executes the instruction, or the computer compiles the instruction and then executes the corresponding compiled program, or the computer reads and executes the instruction, or the computer reads and installs the instruction and then executes the corresponding installed program. Computer-readable media herein can be any available computer-readable storage media or communication media that can be accessed by a computer.

Communication media includes media by which communication signals, including, for example, computer readable instructions, data structures, program modules, or other data, are transmitted from one system to another. Communication media may include conductive transmission media such as cables and wires (e.g., fiber optics, coaxial, etc.) and wireless (non-conductive transmission) media capable of propagating energy waves such as acoustic, electromagnetic, RF, microwave, and infrared. Computer readable instructions, data structures, program modules, or other data may be embodied in a modulated data signal, for example, in a wireless medium such as a carrier wave or similar mechanism such as is embodied as part of spread spectrum techniques. The term "modulated data signal" means a signal that has one or more of its characteristics set or changed in such a manner as to encode information in the signal. The modulation may be analog, digital or hybrid modulation techniques.

By way of example, and not limitation, computer-readable storage media may include volatile and nonvolatile, removable and non-removable media implemented in any method or technology for storage of information such as computer-readable instructions, data structures, program modules or other data. For example, computer-readable storage media include, but are not limited to, volatile memory such as random access memory (RAM, DRAM, SRAM); and non-volatile memory such as flash memory, various read-only memories (ROM, PROM, EPROM, EEPROM), magnetic and ferromagnetic/ferroelectric memories (MRAM, FeRAM); and magnetic and optical storage devices (hard disk, tape, CD, DVD); or other now known media or later developed that can store computer-readable information/data for use by a computer system.

An embodiment according to the present application comprises an apparatus comprising a memory for storing computer program instructions and a processor for executing the program instructions, wherein the computer program instructions, when executed by the processor, trigger the apparatus to perform a method and/or a solution according to the aforementioned embodiments of the present application.

It will be evident to those skilled in the art that the present application is not limited to the details of the foregoing illustrative embodiments, and that the present application may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned. Furthermore, it is obvious that the word "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. A plurality of units or means recited in the apparatus claims may also be implemented by one unit or means in software or hardware. The terms first, second, etc. are used to denote names, but not any particular order.

Claims

1. A method for acquiring microscopic images, applied to a computing device, the computing device establishing a communication connection with a corresponding microscopic device, wherein the method comprises:

acquiring current fluorescence parameter information currently used by the microscopic equipment, wherein the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength;

sending an image acquisition instruction to the microscopic equipment;

2. The method of claim 1, wherein the acquiring current fluorescence parameter information currently being used by the microscopy apparatus comprises:

sending a fluorescence parameter acquisition instruction to the microscopic equipment;

and receiving current fluorescence parameter information returned by the microscopic equipment based on the fluorescence parameter acquisition instruction, wherein the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength.

3. The method of claim 1, wherein the acquiring current fluorescence parameter information currently being used by the microscopy apparatus comprises:

generating a corresponding fluorescence parameter control instruction based on input operation of a user, wherein the fluorescence parameter control instruction is used for adjusting current fluorescence parameter information of the microscopic equipment, and the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength;

wherein the method further comprises:

and sending the fluorescence control instruction to the microscopic equipment.

4. The method of any one of claims 1 to 3, wherein the acquisition region of the specimen includes a plurality of sub-regions, the receiving a sequence of microscope images taken by the microscopy apparatus about the specimen based on the image acquisition instructions to acquire corresponding microscope images comprising:

receiving a microscopic image sequence about the sample, which is shot by the microscopic device based on the image acquisition instruction, wherein the microscopic image sequence comprises images of the plurality of subareas of the sample, each image of the subarea comprises a plurality of microscopic sub-images corresponding to the focal plane heights of the subarea, and the microscopic sub-images correspond to the current excitation light wavelength;

determining three-dimensional coordinate information corresponding to each pixel in each sub-area based on a plurality of microscopic sub-images corresponding to each sub-area;

stitching the images of the plurality of sub-regions to obtain a microscope image corresponding to the current excitation light wavelength.

5. The method of claim 1, wherein the method further comprises:

respective microscopic images are stored with respect to the specimen, wherein each microscopic image corresponds to a current excitation light wavelength acquired when the microscopic image was acquired.

6. The method of claim 5, wherein the method further comprises:

generating an excitation light sequence according to a preset sequence based on each current excitation wavelength;

and displaying the microscopic images corresponding to the excitation light wavelengths based on the excitation light sequence according to a certain time interval.

7. The method of claim 1, wherein the method further comprises:

sending each microscopic image about the sample to a corresponding network device, wherein each microscopic image corresponds to a current excitation light wavelength acquired when the microscopic image was acquired.

8. The method of claim 1, wherein the collection region of the specimen comprises a plurality of sub-regions, wherein the method further comprises:

receiving a plurality of microscopic image sequences about the specimen, taken by the microscopic device based on the image acquisition instructions, wherein each microscopic image sequence comprises an image of at least one subregion of the plurality of subregions of the specimen, and each microscopic image sequence corresponds to a current excitation light wavelength acquired when the microscopic image sequence is acquired;

stitching a microscopic image for the specimen based on the plurality of sequences of microscopic images.

9. The method of claim 8, wherein said stitching a microscopic image for the specimen based on the plurality of microscopic image sequences comprises:

generating images corresponding to the plurality of sub-regions corresponding to each microscopic image sequence according to the image corresponding to each microscopic image sequence, wherein the image corresponding to each sub-region has an excitation light wavelength corresponding to the excitation light wavelength;

stitching a microscopic image about the specimen from the images of the plurality of sub-regions.

10. The method of claim 1, wherein the sending image acquisition instructions to the microscopy device comprises:

and if the current excitation light wavelength meets the image acquisition condition, sending an image acquisition instruction to the microscopic equipment.

11. The method of claim 10, wherein the image acquisition conditions include at least any one of:

the current excitation light wavelength exists in a target excitation light wavelength set;

and the wavelength difference between the current excitation light wavelength and the preamble excitation light wavelength corresponding to the preamble microscopic image reaches a preset wavelength difference.

12. The method according to claim 11, wherein the image acquisition condition includes that a wavelength difference between the current excitation light wavelength and a preamble excitation light wavelength corresponding to a preamble microscopic image reaches a preset wavelength difference; wherein the method further comprises:

and if the preorder microscopic image is received, sending a fluorescence parameter control instruction to the microscopic equipment, wherein the fluorescence parameter control instruction is used for adjusting the current fluorescence parameter information of the microscopic equipment.

13. A method for acquiring microscopic images for use in a microscopic device that establishes a communication connection with a corresponding computing device, wherein the method comprises:

receiving an image acquisition instruction sent by the computing equipment;

sending the sequence of microscopic images to the computing device.

14. The method of claim 13, wherein the method further comprises:

and receiving a fluorescence parameter acquisition instruction sent by the computing equipment, and returning current fluorescence parameter information to the user equipment based on the fluorescence parameter acquisition instruction, wherein the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength.

15. The method of claim 13, wherein the method further comprises:

and receiving a fluorescence parameter control instruction sent by the computing equipment, and adjusting the current fluorescence parameter information of the microscopic equipment based on the fluorescence parameter control instruction, wherein the current fluorescence parameter information is used for determining the corresponding current excitation light wavelength.

16. The method of any of claims 13 to 15, wherein the receiving an image acquisition instruction sent by the computing device comprises:

receiving an image acquisition instruction sent by the computing device, wherein the image acquisition instruction comprises at least one sub-region of a plurality of sub-regions of the specimen;

wherein the acquiring a microscopic image sequence related to the sample based on the image acquisition instruction, wherein the microscopic image sequence includes microscopic sub-images respectively corresponding to one or more sub-regions of the sample, and the microscopic sub-images are used for obtaining the microscopic images corresponding to the sample by stitching, and the acquiring the microscopic image sequence includes:

and acquiring a microscopic image sequence related to the sample based on the image acquisition instruction, wherein the microscopic image sequence comprises microscopic sub-images corresponding to the at least one subregion, and the microscopic sub-images are used for splicing to obtain a microscopic image corresponding to the sample.

17. A method for acquiring a microscopic image, wherein the method comprises:

18. A computing device for acquiring microscopic images, the computing device establishing a communication connection with a corresponding microscopic device, wherein the computing device comprises:

a module, configured to obtain current fluorescence parameter information currently used by the microscopy apparatus, where the current fluorescence parameter information is used to determine a corresponding current excitation light wavelength;

19. A microscopic apparatus for acquiring microscopic images, the microscopic apparatus establishing a communication connection with a corresponding computing apparatus, wherein the microscopic apparatus comprises:

20. An apparatus for acquiring microscopic images, wherein the apparatus comprises:

a processor; and

a memory arranged to store computer executable instructions that, when executed, cause the processor to perform the operations of the method of any of claims 1 to 16.

21. A computer-readable medium storing instructions that, when executed, cause a system to perform the operations of any of the methods of claims 1 to 16.