CN113392675A - Method and equipment for presenting microscopic video information - Google Patents
Method and equipment for presenting microscopic video information Download PDFInfo
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Abstract
The application aims to provide a method and equipment for presenting microscopic video information, which comprises the following steps: acquiring first microscopic image sequence information on a sample, wherein the first microscopic image sequence information comprises a plurality of two-dimensional microscopic image information based on a first temperature sequence, and the first temperature sequence comprises a plurality of temperature assignments of temperature parameters arranged according to a predetermined sequence; generating microscopic video information on the sample according to the first microscopic image sequence information, wherein the arrangement sequence of the two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined sequence; and presenting the microscopic video information. The application can acquire the microscopic video information of the sample about the temperature parameter, the microscopic video information presents the microscopic image of the sample under different assignments of the temperature parameter, sufficient research data are provided for researchers, the contrast effect of the good stroke is achieved, and the use experience of the user is improved.
Description
Technical Field
The application relates to the field of communication, in particular to a technology for presenting microscopic video information.
Background
Microscopic Optical imaging, also commonly referred to as "Optical Microscopy," or "Light Microscopy," refers to a technique whereby visible Light transmitted through or reflected from a sample is passed through one or more lenses to produce a magnified image of the microscopic sample. The image can be observed directly by eyes through an ocular lens, recorded by a light-sensitive plate or a digital image detector such as CCD or CMOS, and displayed and analyzed on a computer. Of course, by combining with the camera device, it is also possible to record a video or the like about the specimen in the field of view. However, the field of view that can be observed by the microscope is limited, and when the size of the sample to be observed exceeds the current field of view, only the condition of the sample in the current field of view can be observed at the same time, and the condition of the sample in the current field of view to be observed includes features that are of interest to researchers, and thus, the efficiency of visual observation is low, and mistakes and omissions are likely to occur.
Disclosure of Invention
It is an object of the present application to provide a method and apparatus for presenting microscopic video information.
According to one aspect of the present application, there is provided a method of presenting microscopic video information, the method comprising:
acquiring first microscopic image sequence information on a sample, wherein the first microscopic image sequence information comprises a plurality of two-dimensional microscopic image information based on a first temperature sequence, and the first temperature sequence comprises a plurality of temperature assignments of temperature parameters arranged according to a predetermined sequence;
generating microscopic video information on the sample according to the first microscopic image sequence information, wherein the arrangement sequence of the two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined sequence;
and presenting the microscopic video information.
According to another aspect of the application, there is provided an apparatus for presenting microscopic video information, the apparatus comprising:
a module for obtaining first microscopic image sequence information about a sample, wherein the first microscopic image sequence information includes a plurality of two-dimensional microscopic image information based on a first temperature sequence, and the first temperature sequence includes a plurality of temperature assignments of temperature parameters arranged in a predetermined order;
a second module, configured to generate microscopic video information about the sample according to the first microscopic image sequence information, where an arrangement order of two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined order;
and the three modules are used for presenting the microscopic video information.
According to an aspect of the present application, there is provided an apparatus for presenting microscopic video information, the apparatus comprising:
a processor; and
a memory arranged to store computer executable instructions that, when executed, cause the processor to perform the operations of any of the methods described above.
According to another aspect of the application, there is provided a computer readable medium storing instructions that, when executed, cause a system to perform the operations of any of the methods described above.
Compared with the prior art, the method and the device have the advantages that first microscopic image sequence information about a sample is obtained, wherein the first microscopic image sequence information comprises a plurality of two-dimensional microscopic image information based on a first temperature sequence, and the first temperature sequence comprises a plurality of temperature assignments of temperature parameters arranged in a preset sequence; generating microscopic video information on the sample according to the first microscopic image sequence information, wherein the arrangement sequence of the two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined sequence; and presenting the microscopic video information. The application can acquire the microscopic video information of the sample about the temperature parameter, the microscopic video information presents the microscopic image of the sample under different assignments of the temperature parameter, sufficient research data are provided for researchers, a good contrast effect is formed, and the use experience of a user is improved.
Drawings
Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings in which:
FIG. 1 shows a flow diagram of a method of presenting microscopic video information according to one embodiment of the present application;
FIG. 2 shows functional modules of an apparatus for presenting microscopic video information according to another embodiment of the present application;
FIG. 3 illustrates an exemplary system that can be used to implement the various embodiments described in this application.
The same or similar reference numbers in the drawings identify the same or similar elements.
Detailed Description
The present application is described in further detail below with reference to the attached figures.
In a typical configuration of the present application, the terminal, the device serving the network, and the trusted party each include one or more processors (e.g., Central Processing Units (CPUs)), input/output interfaces, network interfaces, and memory.
The Memory may include forms of volatile Memory, Random Access Memory (RAM), and/or non-volatile Memory in a computer-readable medium, such as Read Only Memory (ROM) or Flash Memory. Memory is an example of a computer-readable medium.
Computer-readable media, including both non-transitory and non-transitory, removable and non-removable media, may implement information storage by any method or technology. The information may be computer readable instructions, data structures, modules of a program, or other data. Examples of computer storage media include, but are not limited to, Phase-Change Memory (PCM), Programmable Random Access Memory (PRAM), Static Random-Access Memory (SRAM), Dynamic Random Access Memory (DRAM), other types of Random Access Memory (RAM), Read Only Memory (ROM), electrically Erasable Programmable Read-Only Memory (EEPROM), flash Memory or other Memory technology, Compact Disc Read-Only Memory (CD-ROM), Digital Versatile Discs (DVD) or other optical storage, magnetic cassettes, magnetic tape, magnetic disk storage or other magnetic storage devices, or any other non-transmission medium, which can be used to store information that can be accessed by a computing device.
The device referred to in this application includes, but is not limited to, a user device, a network device, or a device formed by integrating a user device and a network device through a network. The user equipment includes, but is not limited to, any mobile electronic product, such as a smart phone, a tablet computer, etc., capable of performing human-computer interaction with a user (e.g., human-computer interaction through a touch panel), and the mobile electronic product may employ any operating system, such as an Android operating system, an iOS operating system, etc. The network Device includes an electronic Device capable of automatically performing numerical calculation and information processing according to a preset or stored instruction, and the hardware includes, but is not limited to, a microprocessor, an Application Specific Integrated Circuit (ASIC), a Programmable Logic Device (PLD), a Field Programmable Gate Array (FPGA), a Digital Signal Processor (DSP), an embedded Device, and the like. The network device includes but is not limited to a computer, a network host, a single network server, a plurality of network server sets or a cloud of a plurality of servers; here, the Cloud is composed of a large number of computers or web servers based on Cloud Computing (Cloud Computing), which is a kind of distributed Computing, one virtual supercomputer consisting of a collection of loosely coupled computers. Including, but not limited to, the internet, a wide area network, a metropolitan area network, a local area network, a VPN network, a wireless Ad Hoc network (Ad Hoc network), etc. Preferably, the device may also be a program running on the user device, the network device, or a device formed by integrating the user device and the network device, the touch terminal, or the network device and the touch terminal through a network.
Of course, those skilled in the art will appreciate that the foregoing is by way of example only, and that other existing or future devices, which may be suitable for use in the present application, are also encompassed within the scope of the present application and are hereby incorporated by reference.
In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.
Fig. 1 shows a method of presenting microscopic video information according to an aspect of the present application, wherein the method is adapted for a computing device comprising a display means, the method comprising step S101, step S102 and step S103. In step S101, a computing device acquires first microscopic image sequence information on a specimen, wherein the first microscopic image sequence information includes a plurality of two-dimensional microscopic image information based on a first temperature sequence including a plurality of temperature assignments of temperature parameters arranged in a predetermined order; in step S102, the computing device generates microscopic video information on the specimen from the first microscopic image sequence information, wherein an arrangement order of two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined order; in step S103, the computing device presents the microscopic video information. The method can be applied to a computing device including, but not limited to, a user device including, but not limited to, any terminal capable of human-computer interaction with a user (e.g., human-computer interaction via a touch pad), a network device including, but not limited to, a computer, a network host, a single network server, a plurality of network server sets, or a cloud including a plurality of servers, or a device formed by integrating the user device and the network device via a network.
Specifically, in step S101, the computing device acquires first microscopic image sequence information on the specimen, wherein the first microscopic image sequence information includes a plurality of two-dimensional microscopic image information based on a first temperature sequence including a plurality of temperature assignments of temperature parameters arranged in a specific order. For example, the first microscopic image sequence includes a plurality of two-dimensional microscopic image information based on the first temperature sequence about the specimen, wherein each two-dimensional microscopic image information includes an image of an entire scanning area of the specimen, such as an entire image spliced from sub-images corresponding to a plurality of scanning areas. The two-dimensional microscopic image information comprises image information of an integral area formed by depth-of-field fusion based on microscopic image information of the sample under different focal plane heights, wherein the depth-of-field fusion is used for taking pixels corresponding to the depth of field of each piece of microscopic image information in the microscopic image information under different focal plane heights, and splicing the pixels to obtain an image with high definition in the integral range. The first temperature sequence comprises a plurality of temperature assignments of temperature parameters arranged according to a specific sequence, for example, the specific sequence includes but is not limited to the size sequence of the temperature assignments, the acquisition time sequence or the sequence set by a user, for example, the temperature sequences arranged according to the size sequence are 5 ℃, 6 ℃, 7 ℃, … 24 ℃, 25 ℃ and the like, and the corresponding temperature sequences are arranged according to the sequence, can be linearly arranged at equal intervals, and can also comprise a part of non-linear sequences with unequal intervals; of course, there may be a corresponding one or more two-dimensional microscopic image information for each temperature assignment in the first temperature sequence information. The computing device acquires first microscopic image sequence information about the sample, for example, the computing device establishes a communication connection with other devices (such as user equipment, network equipment or microscopic equipment, etc.), the other device side stores the first microscopic image sequence information about the sample, and the computing device can receive the first microscopic image sequence information sent by the other devices through the communication connection; alternatively, the computing device can generate first microscopic image sequence information about the specimen from microscopic sub-images of different sub-regions (e.g., scanning regions, etc.) of the specimen, which may be acquired via a communication connection with other devices (e.g., user devices, network devices, or microscopic devices, etc.), or the like.
In step S102, the computing device generates microscopic video information on the specimen from the first microscopic image sequence information, wherein an arrangement order of two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined order. For example, the computing device generates corresponding microscopic video information based on first microscopic image sequence information about the sample, such as arranging each video frame in the microscopic video information according to a plurality of two-dimensional microscopic image information in the first microscopic image sequence in a specific order, wherein the specific order is the same as the order of each temperature assignment in the first temperature sequence, and if a plurality of corresponding two-dimensional microscopic image information exist in a temperature assignment, then the plurality of two-dimensional microscopic image information corresponding to the temperature assignment is arranged according to the acquisition time order, or one or more preferred two-dimensional microscopic image information is selected from the plurality of two-dimensional microscopic image information and the preferred two-dimensional microscopic image information is used for generating the microscopic video information, and the like.
In step S103, the computing device presents the microscopic video information. For example, the computing device comprises a display means for presenting the microscopic video information, such as a display screen or a projector or the like. The computing device can present the microscopic video information through the display device, such as two-dimensional microscopic image information in the microscopic video information arranged according to a specific sequence is presented frame by frame.
In some embodiments, the temperature intervals between each two adjacent temperature assignments of the plurality of temperature assignments are not identical. For example, the arrangement of the temperature assignments of the temperature parameters in the first temperature sequence is non-linear, i.e. the temperature intervals between two adjacent temperature assignments are not exactly the same. If a certain sample has obvious change between 10 ℃ and 15 ℃, has slow change between 0 ℃ and 10 ℃ and 15 ℃ and 25 ℃ and the like, a plurality of temperature assignments (such as 0 ℃, 1 ℃, 2 ℃, … 10 ℃, 15 ℃, 16 ℃, … 25 ℃ and the like) are obtained at intervals of every 1 ℃ in a section with slow change, a plurality of temperature assignments (such as 10 ℃, 10.5 ℃, 11 ℃, …, 15 ℃ and the like) are obtained at intervals of every 0.5 ℃ in a section with slow change, and all the temperature assignments are arranged according to the sequence to obtain corresponding two-dimensional microscopic image information. For example, if a sample changes significantly at a temperature of 10 ℃ to 15 ℃ and slowly at 0 ℃ to 10 ℃ and 15 ℃ to 25 ℃, the temperature interval between each temperature assignment and the adjacent temperature assignment is set according to the corresponding change amount, and if the change amount decreases twice around 12.5 ℃, a first temperature sequence with unequal intervals of 0 ℃, 5 ℃, 8 ℃, 10 ℃, 11 ℃, 11.5 ℃, 11.8 ℃, 12 ℃, 12.1 ℃, 12.2 ℃, … 13 ℃, 13.2 ℃, 13.5 ℃, 14 ℃, 15 ℃, 17 ℃, 20 ℃, 25 ℃ and the like can be taken. Of course, those skilled in the art will appreciate that the first temperature sequence described above is merely exemplary, and that other existing or future first temperature sequences, as may be suitable for use in the present application, are also intended to be encompassed within the scope of the present application and are hereby incorporated by reference.
In some embodiments, the method further comprises step S104 (not shown), in step S104, the computing device obtaining a regulation instruction regarding the microscopic video information, regulating the microscopic video information based on the regulation instruction; in step S103, the computing device presents the regulated and controlled microscopic video information. For example, after the computing device acquires the corresponding microscopic video information, it may acquire the corresponding control instruction based on the microscopic video information, such as identifying the target video frame in the microscopic video information according to the characteristic information of the sample, including but not limited to all the characteristics of the sample or part of the characteristic information of the changed part in the sample; for example, after the computing device presents the microscopic video information, the computing device may acquire a control operation of the user on the microscopic video information, and generate a control instruction on the display video information based on the control operation of the user. And the computing equipment regulates and controls the microscopic video information based on the regulating and controlling instruction, and presents the regulated and controlled microscopic video information and the like. In some embodiments, the regulatory instructions include, but are not limited to: performing a zoom operation on the microscopic video information; adjusting the playing speed of the microscopic video information; switching image dimension information of a specific area of the sample in the microscopic video information from a two-dimensional image to a three-dimensional image, wherein the specific area is contained in the whole area of the sample; adjusting the assignment of other microscopic parameters of the microscopic video information; and adjusting the first temperature sequence into a second temperature sequence, wherein second microscopic image sequence information corresponding to the second temperature sequence is used for generating the regulated and controlled microscopic video information. For example, the conditioning instructions are used to condition the microscopic video information, including but not limited to: 1) performing a zooming operation on the microscopic video information, such as enlarging and carefully watching a part of the microscopic video information, or reducing the playing magnification of the microscopic video information to increase the field of view of a currently played sample; 2) adjusting the playing speed of the microscopic video information, for example, slowing down the playing speed of the microscopic video information at the part which is interested by the user or contains the characteristic information of the sample, and speeding up the playing speed of the microscopic video information at the part which is not interested by the user or does not contain the characteristic information of the sample; 3) switching image dimension information of a specific region of the specimen in the microscopic video information from a two-dimensional image to a three-dimensional image, wherein the specific region is included in the entire region of the specimen, such as a selected region of a user as the specific region or a portion containing characteristic information of the specimen as the specific region, switching a two-dimensional image of the specific region in the microscopic video information to a three-dimensional image, such as acquiring three-dimensional microscopic image sequence information on the specific region from another device, and acquiring two-dimensional three-dimensional integrated microscopic image information of the specimen in which a height of the two-dimensional image coincides with a certain plane (such as a reference plane having a height of zero) of the three-dimensional image portion, and then generating microscopic image information on the specimen based on the two-dimensional three-dimensional integrated microscopic image information of the specimen, wherein, the specific area may be a part of the whole area of the sample, and certainly, the specific area may also be the whole area of the sample, at this time, the two-dimensional three-dimensional comprehensive microscopic image information does not include a two-dimensional image, that is, the corresponding microscopic video information is generated according to the three-dimensional microscopic image information of the whole area of the sample; 4) adjusting assignment of other microscopic parameters of the microscopic video information, such as temperature as a main microscopic parameter of the current microscopic video information, the same assignment of other microscopic parameters of the obtained two-dimensional microscopic image information, and obtaining assignment of other microscopic parameters by the computing device based on user input operation, etc., and obtaining two-dimensional microscopic image information under the assignment corresponding to the other microscopic parameters by the computing device if the assignment of the other microscopic parameters is different from the current assignment, and obtaining adjusted microscopic image information based on the two-dimensional microscopic image information, wherein the other microscopic parameters include, but are not limited to, shooting time information, focal plane height information, rotation angle information, pitch angle information, yaw angle information, illumination lamp brightness information, illumination lamp color information, humidity information, pH value information, fluorescence band information, polarization angle information, and the like, DIC rotation angle information, etc.; 5) and adjusting the first temperature sequence into a second temperature sequence, wherein second microscopic image sequence information corresponding to the second temperature sequence is used for generating regulated and controlled microscopic video information, for example, a sensitive temperature interval in the first temperature sequence is automatically identified by a computing device or based on an input operation of a user, so as to generate the corresponding second temperature sequence, the computing device acquires second image sequence information related to the second temperature sequence, and generates adjusted microscopic video information according to the second image sequence information. Here, one or any combination of the foregoing examples of any regulation instruction may be included in the regulation instruction, and the regulation instruction may be generated based on an operation of a user, or may be determined by a computing device according to a feature recognition performed on the microscopic video information and a recognition result.
In some embodiments, the adjustment instructions include adjusting the second temperature sequence to a second temperature sequence, wherein second microscopic image sequence information corresponding to the second temperature sequence is used to generate adjusted microscopic video information; in step S104, the computing device obtains a control operation of the user on the microscopic video information, generates a control instruction on the microscopic video information based on the control operation, and controls the microscopic video information based on the control instruction, wherein the control operation includes an input operation of the second temperature sequence. For example, the manipulation operation includes an input operation of the second temperature sequence, where the input operation may be to input an increased assignment or a decreased assignment of the second temperature sequence relative to the second temperature sequence, or to input a complete second temperature sequence, and the like, and based on the input operation of the user, the computing device may determine the corresponding second temperature sequence, such as to increase or decrease the input temperature assignment from the first temperature sequence, or to determine the complete second temperature sequence according to the input assignment, and determine the adjusted microscopic video information according to the second temperature sequence, and the like. In other embodiments, the adjusting instructions include adjusting the first temperature sequence to a second temperature sequence, wherein microscopic image sequence information corresponding to the second temperature sequence is used for generating adjusted microscopic video information; in step S104, the computing device performs target identification on the microscopic video information based on the target template feature information of the sample, determines a corresponding regulation and control instruction according to the identification result, and regulates and controls the microscopic video information based on the regulation and control instruction. For example, the computing device may further include target template feature information of the sample, identify a video frame in the microscopic video information according to the target template feature information, and determine a corresponding second temperature sequence according to the identification result; if the sample is a certain cell with a specific number, the change of the cell (such as whether the cell is a pathological change or not) is obtained after the cell is cultured in a culture dish for a certain time, the feature of the pathological change cell is taken as target template feature information, target identification is carried out on each video frame in the microscopic video information, if the target template feature information exists in the obtained video frame, the temperature assignment corresponding to the video frame is taken as pathological change temperature assignment, then, the corresponding second temperature sequence is determined according to the pathological change temperature assignment, for example, the pathological change temperature assignment is directly taken as the second temperature sequence, or the maximum value and the minimum value in the pathological change temperature assignment are taken, the second temperature sequence is re-determined at a certain temperature interval, and the like, further, the pathological change temperature sequence can be determined according to the maximum value and the minimum value in the pathological change temperature assignment, the corresponding interval is determined based on the number (such as the number of the pathological change cell and the like) of the target template feature information in the video frame corresponding to the pathological change temperature assignment, and the second temperature sequence is determined, and the like, so that the second temperature sequence is determined And (4) columns.
In some embodiments, the performing target identification on the microscopic video information based on the target template features of the sample, and determining the corresponding control instruction according to the identification result includes: and carrying out target identification on the microscopic video information based on the target template characteristic information of the sample, and determining a plurality of video frames containing the target characteristic information in the microscopic video information. For example, the microscopic video information is subjected to target recognition based on the target template feature information of the sample, video frames containing the target template feature information in the microscopic video information are determined, and corresponding second temperature sequences are determined according to temperature assignments corresponding to the video frames, for example, the temperature assignments corresponding to the video frames are used as the corresponding second temperature sequences, and the like. In some embodiments, the determining the corresponding second temperature sequence according to the corresponding temperature assignments for the plurality of video frames includes: determining a corresponding temperature characteristic change curve according to the relevance information of the video frames and the target template characteristic information; and determining a minimum temperature assignment and a maximum temperature assignment of the temperature assignments corresponding to the plurality of video frames, and determining a corresponding second temperature sequence based on the temperature characteristic change curve, the minimum temperature assignment and the maximum temperature assignment. For example, the association degree information of the video frame and the target template feature information includes, but is not limited to, similarity degree information with the target template feature information or the number of objects satisfying the target template feature information in the video frame, and the like, a corresponding temperature feature change curve is determined based on the association degree information, if the temperature assignment is used as the X axis and the correlation degree information is used as the Y axis to describe the corresponding temperature characteristic change curve, determining a corresponding second temperature sequence according to the minimum temperature assignment and the maximum temperature assignment of the temperature assignments in the video frame and the temperature characteristic change curve, if a certain correlation interval is taken, the temperature assignment corresponding to the correlation value on the temperature characteristic change curve is taken as the temperature assignment of the temperature parameter of the second temperature sequence, and the like, of course, if the change of the correlation value is large, the temperature assignment may take a larger amount of two-dimensional microscopic image information as a corresponding video frame, etc.
In some embodiments, the manipulating the microscopic video information based on the manipulation instruction comprises: acquiring a second microscopic image sequence related to the sample based on the second temperature sequence, wherein the second microscopic image sequence comprises a plurality of two-dimensional microscopic image information based on the second temperature sequence, and the second temperature sequence comprises a plurality of temperature assignments of temperature parameters arranged in a predetermined order; and generating regulated and controlled microscopic video information according to the second microscopic image sequence information, wherein the two-dimensional microscopic image information arrangement sequence of the regulated and controlled microscopic video information corresponds to the preset sequence. For example, after the computing device acquires the corresponding second temperature sequence, the computing device acquires the corresponding second microscopic image sequence based on the second temperature sequence, for example, the computing device establishes a communication connection with another device (e.g., a user device, a network device, or a microscopic device, etc.), the other device side stores second microscopic image sequence information about the sample, and the computing device can receive the second microscopic image sequence information sent by the other device through the communication connection; alternatively, the computing device can generate second microimage sequence information about the specimen from microsub-images of different sub-regions (e.g., scan regions, etc.) of the specimen, which may be acquired via a communication connection with other devices (e.g., user devices, network devices, or microscopy devices, etc.), etc. Subsequently, the computing device arranges the video frames in the microscopic video information according to a specific sequence according to the two-dimensional microscopic image information in the second microscopic image sequence, wherein the specific sequence is the same as the sequence of the temperature assignments in the second temperature sequence.
In some embodiments, the acquiring the second sequence of microscope images for the specimen based on the second temperature sequence comprises: sending a microscopic image request about the specimen to a corresponding other device, wherein the microscopic image request comprises identification information of the specimen and the second temperature sequence; and receiving a second microscopic image sequence which is returned by the other equipment and is about the sample, wherein the second microscopic image sequence is corresponding to the second temperature sequence. For example, the identification information of the specimen includes an identifier or the like for determining corresponding microscopic video information, including but not limited to a plurality of microscopic image information of the specimen, such as a plurality of microscopic image information about the specimen, based on which the network device can acquire the corresponding microscopic video information; or, the identification information includes a key field of the sample, such as a name of the sample or a keyword extracted from the name of the sample for searching the sample; the identification information comprises microscopic record information of the sample, such as a historical record of microscopic image information or microscopic video information about the sample uploaded or searched by a user in an application; the unique identification code information of the sample, such as the unique identification code set by the sample in application, and the like; the identification information may include a plurality of image information of the specimen, such as the network device may identify the corresponding specimen in a database based on the image information. The computing device sends a microscopic image request to other devices (such as other user devices, network devices or microscopic devices) and the request comprises the identification information of the sample and second temperature sequence information, the other devices receive the microscopic image request and acquire corresponding second image sequence information in the database according to the identification information of the sample and the second temperature sequence, the temperature assignment of the two-dimensional microscopic image information in the second image sequence information corresponds to the second temperature sequence, and the environmental parameters caused by other microscopic parameters are the same.
In some embodiments, the obtaining the second sequence of microscope images for a substantial sample based on the second temperature sequence comprises: sending an acquisition instruction about the sample to a corresponding microscopic device, wherein the acquisition instruction is used for controlling the environment temperature of the sample when the sample is acquired to correspond to the plurality of temperature assignments in the second temperature sequence, and acquiring microscopic image information of the sample at the environment temperature; and receiving a second microscopic image sequence about the sample, which is sent by the microscopic device, wherein the second microscopic image sequence corresponds to the second temperature sequence. For example, after the computing device determines the corresponding second temperature sequence, it may also send an acquisition instruction to the microscopy apparatus based on the second temperature sequence, and control the microscopy apparatus to acquire image information about the sample in the second temperature sequence, and the like, where the acquisition instruction includes the second temperature sequence and is used to control the ambient temperature of the sample when being acquired to be consistent with the temperature assignments in the second temperature sequence, so that each temperature assignment can acquire microscopic image information about the sample. Subsequently, the computing device receives a second sequence of microscopic images, etc., of the specimen, acquired by the microscopic device.
Fig. 2 shows an apparatus 100 for presenting microscopic video information according to an aspect of the present application, the apparatus comprising a one-module 101, a two-module 102, and a three-module 103. A module 101 for obtaining first microscopic image sequence information about a sample, wherein the first microscopic image sequence information includes a plurality of two-dimensional microscopic image information based on a first temperature sequence, and the first temperature sequence includes a plurality of temperature assignments of temperature parameters arranged in a predetermined order; a second module 102, configured to generate microscopic video information about the sample according to the first microscopic image sequence information, wherein an arrangement order of two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined order; and a third module 103 for presenting the microscopic video information. Here, the corresponding embodiments of the one-to-one module 101, the two-to-two module 102, and the one-to-three module 103 in fig. 2 are the same as or similar to the specific embodiments of the step S101, the step S102, and the step S103 in fig. 1, and are not repeated here, but are included herein by reference.
In some embodiments, the temperature intervals between each two adjacent temperature assignments of the plurality of temperature assignments are not identical. Here, the embodiment corresponding to the temperature interval between every two adjacent temperature assignments not being identical is the same as or similar to the specific embodiment described above, and the detailed description thereof is omitted, and the embodiment is included herein by way of reference.
In some embodiments, the apparatus further comprises a fourth module 104 (not shown) for obtaining a regulation instruction regarding the microscopic video information, and regulating the microscopic video information based on the regulation instruction; and the three modules 103 are used for presenting the regulated and controlled microscopic video information. In some embodiments, the regulatory instructions include, but are not limited to: performing a zoom operation on the microscopic video information; adjusting the playing speed of the microscopic video information; switching image dimension information of a specific area of the sample in the microscopic video information from a two-dimensional image to a three-dimensional image, wherein the specific area is contained in the whole area of the sample; adjusting the assignment of other microscopic parameters of the microscopic video information; and adjusting the first temperature sequence into a second temperature sequence, wherein second microscopic image sequence information corresponding to the second temperature sequence is used for generating the regulated and controlled microscopic video information. Here, the implementation of the regulation instruction is the same as or similar to the specific implementation of the regulation instruction, and is not repeated herein and is included by reference.
In some embodiments, the adjustment instructions include adjusting the first temperature sequence to a second temperature sequence, wherein second microscopic image sequence information corresponding to the second temperature sequence is used to generate adjusted microscopic video information; a fourth module 104, configured to obtain a control operation of the user on the microscopic video information, generate a control instruction on the microscopic video information based on the control operation, and control the microscopic video information based on the control instruction, where the control operation includes an input operation of the second temperature sequence. In other embodiments, the adjusting instructions include adjusting the first temperature sequence to a second temperature sequence, wherein microscopic image sequence information corresponding to the second temperature sequence is used for generating adjusted microscopic video information; the fourth module 104 is configured to perform target identification on the microscopic video information based on the target template feature information of the sample, determine a corresponding regulation instruction according to an identification result, and regulate and control the microscopic video information based on the regulation instruction. In some embodiments, the performing target identification on the microscopic video information based on the target template features of the sample, and determining the corresponding control instruction according to the identification result includes: and carrying out target identification on the microscopic video information based on the target template characteristic information of the sample, and determining a plurality of video frames containing the target characteristic information in the microscopic video information. In some embodiments, the determining the corresponding second temperature sequence according to the corresponding temperature assignments for the plurality of video frames includes: determining a corresponding temperature characteristic change curve according to the relevance information of the video frames and the target template characteristic information; and determining a minimum temperature assignment and a maximum temperature assignment of the temperature assignments corresponding to the plurality of video frames, and determining a corresponding second temperature sequence based on the temperature characteristic change curve, the minimum temperature assignment and the maximum temperature assignment. Here, the embodiment of the regulation instruction corresponding to the adjustment of the first temperature sequence to the second temperature sequence is the same as or similar to the specific embodiment of the regulation instruction corresponding to the adjustment of the first temperature sequence to the second temperature sequence, and is not repeated and is included herein by reference.
In some embodiments, the manipulating the microscopic video information based on the manipulation instruction comprises: acquiring a second microscopic image sequence related to the sample based on the second temperature sequence, wherein the second microscopic image sequence comprises a plurality of two-dimensional microscopic image information based on the second temperature sequence, and the second temperature sequence comprises a plurality of temperature assignments of temperature parameters arranged in a predetermined order; and generating regulated and controlled microscopic video information according to the second microscopic image sequence information, wherein the two-dimensional microscopic image information arrangement sequence of the regulated and controlled microscopic video information corresponds to the preset sequence. In some embodiments, the acquiring the second sequence of microscope images for the specimen based on the second temperature sequence comprises: sending a microscopic image request about the specimen to a corresponding other device, wherein the microscopic image request comprises identification information of the specimen and the second temperature sequence; and receiving a second microscopic image sequence which is returned by the other equipment and is about the sample, wherein the second microscopic image sequence is corresponding to the second temperature sequence. In some embodiments, the obtaining the second sequence of microscope images for a substantial sample based on the second temperature sequence comprises: sending an acquisition instruction about the sample to a corresponding microscopic device, wherein the acquisition instruction is used for controlling the environment temperature of the sample when the sample is acquired to correspond to the plurality of temperature assignments in the second temperature sequence, and acquiring microscopic image information of the sample at the environment temperature; and receiving a second microscopic image sequence about the sample, which is sent by the microscopic device, wherein the second microscopic image sequence corresponds to the second temperature sequence. Here, the embodiment corresponding to the regulation and control of the microscopic video information based on the regulation and control instruction is the same as or similar to the specific embodiment of the regulation and control of the microscopic video information based on the regulation and control instruction, and is not repeated here, and is included herein by way of reference.
In addition to the methods and apparatus described in the embodiments above, the present application also provides a computer readable storage medium storing computer code that, when executed, performs the method as described in any of the preceding claims.
The present application also provides a computer program product, which when executed by a computer device, performs the method of any of the preceding claims.
The present application further provides a computer device, comprising:
one or more processors;
a memory for storing one or more computer programs;
the one or more computer programs, when executed by the one or more processors, cause the one or more processors to implement the method of any preceding claim.
FIG. 3 illustrates an exemplary system that can be used to implement the various embodiments described herein;
in some embodiments, as shown in FIG. 3, the system 300 can be implemented as any of the above-described devices in the various embodiments. In some embodiments, system 300 may include one or more computer-readable media (e.g., system memory or NVM/storage 320) having instructions and one or more processors (e.g., processor(s) 305) coupled with the one or more computer-readable media and configured to execute the instructions to implement modules to perform the actions described herein.
For one embodiment, system control module 310 may include any suitable interface controllers to provide any suitable interface to at least one of processor(s) 305 and/or any suitable device or component in communication with system control module 310.
The system control module 310 may include a memory controller module 330 to provide an interface to the system memory 315. Memory controller module 330 may be a hardware module, a software module, and/or a firmware module.
System memory 315 may be used, for example, to load and store data and/or instructions for system 300. For one embodiment, system memory 315 may include any suitable volatile memory, such as suitable DRAM. In some embodiments, the system memory 315 may include a double data rate type four synchronous dynamic random access memory (DDR4 SDRAM).
For one embodiment, system control module 310 may include one or more input/output (I/O) controllers to provide an interface to NVM/storage 320 and communication interface(s) 325.
For example, NVM/storage 320 may be used to store data and/or instructions. NVM/storage 320 may include any suitable non-volatile memory (e.g., flash memory) and/or may include any suitable non-volatile storage device(s) (e.g., one or more Hard Disk Drives (HDDs), one or more Compact Disc (CD) drives, and/or one or more Digital Versatile Disc (DVD) drives).
NVM/storage 320 may include storage resources that are physically part of the device on which system 300 is installed or may be accessed by the device and not necessarily part of the device. For example, NVM/storage 320 may be accessible over a network via communication interface(s) 325.
Communication interface(s) 325 may provide an interface for system 300 to communicate over one or more networks and/or with any other suitable device. System 300 may wirelessly communicate with one or more components of a wireless network according to any of one or more wireless network standards and/or protocols.
For one embodiment, at least one of the processor(s) 305 may be packaged together with logic for one or more controller(s) (e.g., memory controller module 330) of the system control module 310. For one embodiment, at least one of the processor(s) 305 may be packaged together with logic for one or more controller(s) of the system control module 310 to form a System In Package (SiP). For one embodiment, at least one of the processor(s) 305 may be integrated on the same die with logic for one or more controller(s) of the system control module 310. For one embodiment, at least one of the processor(s) 305 may be integrated on the same die with logic for one or more controller(s) of the system control module 310 to form a system on a chip (SoC).
In various embodiments, system 300 may be, but is not limited to being: a server, a workstation, a desktop computing device, or a mobile computing device (e.g., a laptop computing device, a handheld computing device, a tablet, a netbook, etc.). In various embodiments, system 300 may have more or fewer components and/or different architectures. For example, in some embodiments, system 300 includes one or more cameras, a keyboard, a Liquid Crystal Display (LCD) screen (including a touch screen display), a non-volatile memory port, multiple antennas, a graphics chip, an Application Specific Integrated Circuit (ASIC), and speakers.
It should be noted that the present application may be implemented in software and/or a combination of software and hardware, for example, implemented using Application Specific Integrated Circuits (ASICs), general purpose computers or any other similar hardware devices. In one embodiment, the software programs of the present application may be executed by a processor to implement the steps or functions described above. Likewise, the software programs (including associated data structures) of the present application may be stored in a computer readable recording medium, such as RAM memory, magnetic or optical drive or diskette and the like. Additionally, some of the steps or functions of the present application may be implemented in hardware, for example, as circuitry that cooperates with the processor to perform various steps or functions.
In addition, some of the present application may be implemented as a computer program product, such as computer program instructions, which when executed by a computer, may invoke or provide methods and/or techniques in accordance with the present application through the operation of the computer. Those skilled in the art will appreciate that the form in which the computer program instructions reside on a computer-readable medium includes, but is not limited to, source files, executable files, installation package files, and the like, and that the manner in which the computer program instructions are executed by a computer includes, but is not limited to: the computer directly executes the instruction, or the computer compiles the instruction and then executes the corresponding compiled program, or the computer reads and executes the instruction, or the computer reads and installs the instruction and then executes the corresponding installed program. Computer-readable media herein can be any available computer-readable storage media or communication media that can be accessed by a computer.
Communication media includes media by which communication signals, including, for example, computer readable instructions, data structures, program modules, or other data, are transmitted from one system to another. Communication media may include conductive transmission media such as cables and wires (e.g., fiber optics, coaxial, etc.) and wireless (non-conductive transmission) media capable of propagating energy waves such as acoustic, electromagnetic, RF, microwave, and infrared. Computer readable instructions, data structures, program modules, or other data may be embodied in a modulated data signal, for example, in a wireless medium such as a carrier wave or similar mechanism such as is embodied as part of spread spectrum techniques. The term "modulated data signal" means a signal that has one or more of its characteristics set or changed in such a manner as to encode information in the signal. The modulation may be analog, digital or hybrid modulation techniques.
By way of example, and not limitation, computer-readable storage media may include volatile and nonvolatile, removable and non-removable media implemented in any method or technology for storage of information such as computer-readable instructions, data structures, program modules or other data. For example, computer-readable storage media include, but are not limited to, volatile memory such as random access memory (RAM, DRAM, SRAM); and non-volatile memory such as flash memory, various read-only memories (ROM, PROM, EPROM, EEPROM), magnetic and ferromagnetic/ferroelectric memories (MRAM, FeRAM); and magnetic and optical storage devices (hard disk, tape, CD, DVD); or other now known media or later developed that can store computer-readable information/data for use by a computer system.
An embodiment according to the present application comprises an apparatus comprising a memory for storing computer program instructions and a processor for executing the program instructions, wherein the computer program instructions, when executed by the processor, trigger the apparatus to perform a method and/or a solution according to the aforementioned embodiments of the present application.
It will be evident to those skilled in the art that the present application is not limited to the details of the foregoing illustrative embodiments, and that the present application may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned. Furthermore, it is obvious that the word "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. A plurality of units or means recited in the apparatus claims may also be implemented by one unit or means in software or hardware. The terms first, second, etc. are used to denote names, but not any particular order.
Claims (14)
1. A method of presenting microscopic video information, wherein the method comprises:
acquiring first microscopic image sequence information about a sample, wherein the first microscopic image sequence information comprises a plurality of two-dimensional microscopic image information based on a first temperature sequence, and the first temperature sequence comprises a plurality of temperature assignments of temperature parameters according to a specific predetermined sequence;
generating microscopic video information on the sample according to the first microscopic image sequence information, wherein the arrangement sequence of the two-dimensional microscopic image information in the microscopic video information corresponds to the predetermined sequence;
and presenting the microscopic video information.
2. The method of claim 1, wherein the temperature intervals between each two adjacent temperature assignments of the plurality of temperature assignments are not identical.
3. The method according to claim 1 or 2, wherein the method further comprises:
acquiring a regulation and control instruction about the microscopic video information, and regulating and controlling the microscopic video information based on the regulation and control instruction;
wherein said presenting said microscopic video information comprises:
and presenting the regulated and controlled microscopic video information.
4. The method of claim 3, wherein the regulatory instructions comprise at least any one of:
performing a zoom operation on the microscopic video information;
adjusting the playing speed of the microscopic video information;
switching image dimension information of a specific area of the sample in the microscopic video information from a two-dimensional image to a three-dimensional image, wherein the specific area is contained in the whole area of the sample;
adjusting the assignment of other microscopic parameters of the microscopic video information;
and adjusting the first temperature sequence into a second temperature sequence, wherein second microscopic image sequence information corresponding to the second temperature sequence is used for generating the regulated and controlled microscopic video information.
5. The method of claim 4, wherein the adjustment instruction comprises adjusting the first temperature sequence to a second temperature sequence, wherein second microscopic image sequence information corresponding to the second temperature sequence is used for generating adjusted microscopic video information; wherein the obtaining of the regulation instruction about the microscopic video information, and regulating the microscopic video information based on the regulation instruction, includes:
and acquiring a regulation and control operation of a user on the microscopic video information, generating a regulation and control instruction on the microscopic video information based on the regulation and control operation, and regulating and controlling the microscopic video information based on the regulation and control instruction, wherein the regulation and control operation comprises an input operation of the second temperature sequence.
6. The method of claim 4, wherein the adjustment instruction comprises adjusting the first temperature sequence to a second temperature sequence, wherein microscopic image sequence information corresponding to the second temperature sequence is used for generating adjusted microscopic video information; wherein the obtaining of the regulation instruction about the microscopic video information, and regulating the microscopic video information based on the regulation instruction, includes:
and carrying out target identification on the microscopic video information based on the target template characteristic information of the sample, determining a corresponding regulation and control instruction according to an identification result, and regulating and controlling the microscopic video information based on the regulation and control instruction.
7. The method of claim 6, wherein the performing target identification on the microscopic video information based on the target template features of the sample and determining the corresponding control command according to the identification result comprises:
carrying out target identification on the microscopic video information based on the target template characteristic information of the sample, and determining a plurality of video frames containing the target characteristic information in the microscopic video information;
determining a corresponding second temperature sequence according to the temperature assignments corresponding to the plurality of video frames;
and determining a corresponding regulation instruction according to the second temperature sequence.
8. The method of claim 7, wherein said determining a corresponding second temperature sequence based on corresponding temperature assignments for the plurality of video frames comprises:
determining a corresponding temperature characteristic change curve according to the relevance information of the video frames and the target template characteristic information;
and determining a minimum temperature assignment and a maximum temperature assignment of the temperature assignments corresponding to the plurality of video frames, and determining a corresponding second temperature sequence based on the temperature characteristic change curve, the minimum temperature assignment and the maximum temperature assignment.
9. The method of any of claims 5 to 8, wherein said conditioning the microscopic video information based on the conditioning instructions comprises:
acquiring a second microscopic image sequence related to the sample based on the second temperature sequence, wherein the second microscopic image sequence comprises a plurality of two-dimensional microscopic image information based on the second temperature sequence, and the second temperature sequence comprises a plurality of temperature assignments of temperature parameters arranged according to a predetermined sequence;
and generating regulated and controlled microscopic video information according to the second microscopic image sequence information, wherein the two-dimensional microscopic image information arrangement sequence of the regulated and controlled microscopic video information corresponds to the preset sequence.
10. The method of claim 9, wherein the acquiring the second sequence of microscopic images for the specimen based on the second temperature sequence comprises:
sending a microscopic image request about the specimen to a corresponding other device, wherein the microscopic image request comprises identification information of the specimen and the second temperature sequence;
receiving a second sequence of microscope images of the sample returned by the other device, wherein the second sequence of microscope images corresponds to the second temperature sequence.
11. The method of claim 9, wherein the acquiring the second sequence of microscopic images for the specimen based on the second temperature sequence comprises:
sending an acquisition instruction about the sample to a corresponding microscopic device, wherein the acquisition instruction is used for controlling the environment temperature of the sample when the sample is acquired to correspond to the plurality of temperature assignments in the second temperature sequence and acquiring microscopic image information of the sample at the environment temperature;
and receiving a second microscopic image sequence about the sample, which is sent by the microscopic device, wherein the second microscopic image sequence corresponds to the second temperature sequence.
12. An apparatus for presenting microscopic video information, wherein the apparatus comprises:
a module for obtaining first microscopic image sequence information about a sample, wherein the first microscopic image sequence information includes a plurality of two-dimensional microscopic image information based on a first temperature sequence, and the first temperature sequence includes a plurality of temperature assignments of temperature parameters arranged in a specific order;
a second module, configured to generate microscopic video information about the sample according to the first microscopic image sequence information, wherein an arrangement order of two-dimensional microscopic image information of the microscopic video information corresponds to the first temperature sequence;
and the three modules are used for presenting the microscopic video information.
13. An apparatus for presenting microscopic video information, wherein the apparatus comprises:
a processor; and
a memory arranged to store computer executable instructions that, when executed, cause the processor to perform the operations of the method of any of claims 1 to 11.
14. A computer-readable medium storing instructions that, when executed, cause a system to perform operations to perform a method as recited in any of claims 1-11.
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