CN113455596A - Fish oil-rich oyster compound feed and preparation method thereof - Google Patents
Fish oil-rich oyster compound feed and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of feeds, in particular to an oyster compound feed rich in fish oil and a preparation method thereof, and solves the problems of prolonged fattening period and slimming of oysters caused by natural reasons. The invention provides an oyster compound feed rich in fish oil, which is characterized by comprising the following raw material components in parts by weight: 2-6 parts of fish oil, 5-10 parts of Isochrysis galbana, 10-20 parts of schizochytrium limacinum, 10-20 parts of spirulina platensis, 15-25 parts of dunaliella salina, 8-12 parts of clostridium ethanolate protein, 0.5-1 part of vitamin complex additive, 0.5-1 part of lipase, 0.5-1 part of sucrose fatty acid ester, 5-8 parts of sodium caseinate and 20-30 parts of corn starch; also provides a preparation method thereof. The invention is used for feeding and fattening oysters.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to an oyster compound feed rich in fish oil and a preparation method thereof.
Background
Oyster is common shellfish in coastal areas of China, called oyster in Fujian and Guangdong, oyster yellow in Zhejiang and Jiangsu, oyster or oyster in Liaoning and Shandong.
The oysters are shellfish with high economic value, rich nutrition and delicious meat taste, dry matter of a soft body part contains 45-57% of protein, 7-11% of fat, 19-38% of glycogen, 200 times higher iodine content than milk or egg yolk, and a large amount of vitamins, and the oyster is a high-protein low-fat marine product, also called as 'marine milk'.
In recent years, with the rapid increase of the breeding amount of oysters, the bearing capacity of a breeding sea area is exceeded, so that the quantity of phytoplankton in the breeding sea area is not enough to support the normal growth, development and fattening of the oysters bred in the sea area, the phenomena of prolonging the fattening period and slimming of the oysters are caused, and great economic loss is brought to farmers.
Oysters are filter-feeding shellfishes and have a strong physical selection of food, i.e., only food with a smaller caliber than that of the oyster is ingested, preferably with food particles within 10 μm in the larval stage of oyster development, and preferably with food particles within 25 μm in the adult stage of oyster development.
The feeds used in the oyster cultivation industry at present are as follows:
(1) the application method of the fresh and alive unicellular algae liquid is that the fresh and alive unicellular algae liquid is directly splashed into an oyster purifying pool.
The advantage is that the fresh and alive unicellular algae can freely move in the water body, the suspension property is good, the oyster can be eaten in, and no waste is caused basically. The defects that the fresh and alive unicellular algae liquid needs to be transported and stored at low temperature, the quality guarantee period is about 7 days, and the popularization and the application are not facilitated; and the adult consumption of oysters is large, for an oyster purification plant, about tens of thousands of jin of oysters enter a purification tank every time, the fresh and alive unicellular algae liquid required to be fed every day is huge, and fresh and alive unicellular algae liquid production enterprises cannot supply the fresh and alive unicellular algae liquid continuously.
(2) The unicellular algae powder is used by adding water, stirring and sprinkling into the oyster purification pool.
The advantage is that the supply volume is big, can satisfy the production demand that the oyster was bred. The disadvantages are poor suspension property and serious waste.
The two feeds are single in component and unbalanced in nutrition.
(3) The oyster feed is used by adding water, stirring, and sprinkling into oyster purification pool.
The advantages are large supply amount and capability of meeting the requirements of oyster purification plants on feeds.
The method has the following defects: the preparation method of the oyster feed in the prior art generally comprises the following steps: weighing raw materials → mixing raw materials → crushing raw materials → micronizing raw materials → packaging finished products. The particle size of feed particles can only be controlled to be about 50 mu m at present due to the limitation of the equipment of an ultrafine grinder, the particle size of food particles suitable for the adult stage of oyster development is within 25 mu m, and the oyster feed produced by the prior art can not meet the requirement of oysters on the particle size of the food particles; and the feed particles can expand after absorbing water, the volume is increased by several times, and the requirement of the oysters on the particle size of the food particles can not be met.
The oyster growth and fattening stage needs to take more energy, and the fish oil is a good choice of energy raw materials, so that a proper amount of fish oil can be added into the oyster feed. However, since the fish oil cannot be mixed with other raw materials in the feed uniformly and causes caking of the feed, it is impossible to add a proper amount of fish oil to the oyster feed.
Disclosure of Invention
Aiming at overcoming the defects of the prior art, the invention provides the fish oil-rich oyster compound feed which has simple and reasonable raw material composition, natural and environment-friendly components, scientific proportion and obvious fattening effect, meets the requirement of oysters on the particle size of food particles, does not expand when meeting water and has good suspension property, and the preparation method thereof aiming at the nutrition required by the fattening stage of oysters.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
the oyster compound feed rich in fish oil is characterized by comprising the following raw material components in parts by weight: 2-6 parts of fish oil, 5-10 parts of Isochrysis galbana, 10-20 parts of schizochytrium limacinum, 10-20 parts of spirulina platensis, 15-25 parts of dunaliella salina, 8-12 parts of clostridium ethanolate protein, 0.5-1 part of vitamin complex additive, 0.5-1 part of lipase, 0.5-1 part of sucrose fatty acid ester, 5-8 parts of sodium caseinate and 20-30 parts of corn starch.
Preferably, the feed consists of the following raw materials in parts by weight: 4 parts of fish oil, 5 parts of Isochrysis galbana, 15 parts of schizochytrium, 15 parts of spirulina platensis, 20 parts of dunaliella salina, 10 parts of clostridium ethanolate protein, 0.5 part of vitamin complex additive, 0.5 part of lipase, 0.5 part of sucrose fatty acid ester, 5 parts of sodium caseinate and 24.5 parts of corn starch.
Preferably, the method comprises the following steps:
(1) respectively weighing fish oil, Isochrysis galbana, schizochytrium, spirulina platensis, dunaliella salina, clostridium ethanolicum protein, a vitamin complex additive, lipase, sucrose fatty acid ester, sodium caseinate and corn starch in parts by weight;
(2) adding fish oil into an oil dissolving pot, heating to 70 ℃, adding the sucrose fatty acid ester weighed in the step (1), and stirring for 30 minutes at constant temperature to obtain oil phase liquid;
(3) adding water 7 times the total weight of the raw materials in the step (1) into an emulsifying tank, heating to 70 ℃, adding the corn starch weighed in the step (1), and stirring at constant temperature for 30 minutes to obtain a corn starch solution;
(4) sequentially adding the isochrysis galbana, schizochytrium limacinum, spirulina platensis, dunaliella salina, clostridium ethanolate protein, a vitamin complex additive, lipase and sodium caseinate weighed in the step (1) into the corn starch solution obtained in the step (3), and stirring at constant temperature for 5 minutes to obtain an aqueous phase liquid;
(5) adding the oil phase liquid obtained in the step (2) into the water phase liquid obtained in the step (4), and stirring for 10 minutes at constant temperature to obtain a crude mixed liquid;
(6) emulsifying the crude mixed liquid obtained in the step (5) by using an on-line high-speed shearing emulsifying machine to obtain fine mixed liquid;
(7) filtering the fine mixed solution obtained in the step (6) by using a duplex filter, wherein the mesh number of a screen is 200 meshes to obtain filtered mixed solution;
(8) homogenizing the filtered mixed liquor obtained in the step (7) by a high-pressure homogenizer at the pressure of 60Mpa to obtain fine mixed liquor;
(9) performing vacuum degassing on the fine mixed solution obtained in the step (8) by using a vacuum degassing pot to obtain degassed mixed solution;
(10) spray drying the degassed mixed solution obtained in the step (9) by using a spray dryer, wherein the air inlet temperature is 130 ℃, and the air outlet temperature is 85 ℃ to obtain a spray-dried semi-finished product;
(11) introducing hot air into the front half part of the spray-dried semi-finished product obtained in the step (10) for auxiliary drying, and introducing dehumidification cold air into the rear half part of the vibration fluidized bed for cooling to obtain a fluidized bed semi-finished product;
(12) and (4) screening the semi-finished product of the fluidized bed obtained in the step (11) by using a vibrating screen, wherein the mesh number of the screen is 350 meshes, so as to obtain the oyster compound feed rich in fish oil.
The invention has the beneficial effects that:
the fish oil-rich oyster compound feed disclosed by the invention is simple and reasonable in raw material composition, low in production cost, natural and environment-friendly in components, scientific in proportioning and balanced in nutrition, and has an obvious short-term fattening effect when being applied to oyster purification and temporary rearing stages.
The fish oil-rich oyster compound feed provided by the invention fully considers the nutritional requirements of oysters in the growth and fattening periods, the selected dinoflagellates, schizochytrium, spirulina platensis and dunaliella are natural biological baits of the oysters in the natural sea area environment, and the added clostridium ethanolicum protein, vitamin complex additive and corn starch provide nutritional ingredients such as protein, amino acid, vitamins, trace elements and energy required by the oyster fattening period.
Wherein:
the fish oil is rich in polyunsaturated fatty acid (PUFA) and becomes an important functional food, and is an important energy raw material, and the energy content of the fish oil is about 39.54MJ/kg, which is 2.25 times of the carbohydrate energy value of the common energy raw material.
The lipase can catalyze the hydrolysis of fish oil to generate fatty acid, glycerol and glyceride, and is convenient for digestion and absorption of oysters. Since the digestive enzymes in the body of oysters are mainly amylase and protease, lipase must be additionally supplemented.
The sucrose fatty acid ester is an emulsifier, which can make the fish oil and other components mixed more uniformly.
The isochrysis galbana belongs to the phylum chrysophyceae, the class of Prymphyceae, the order of Isochrysomyales, the family of Isochrysomylaceae and the genus of Isochrysomyzidae in classification, is a high-quality bait in aquaculture industry, is widely used as a bait for shellfish, sea cucumbers, crustaceans, larval and the like, particularly can be used as an optimal bait for bivalve larvae and juvenile mollusks such as oysters and the like, has much higher total lipid content than green algae and diatoms, and is easier to digest by the shellfish larvae.
The schizochytrium belongs to the phylum Eumycota, class Oomycetes, order Saprolegniales, family Thraustochytridae in classification, is a marine fungus unicellular algae rich in docosahexaenoic acid (DHA), and is a good functional bait, wherein the main components of the algae are lipid, protein and total sugar, and the fat content of the algae can account for more than 50% of the dry weight of cells.
The Spirulina platensis belongs to the phylum Cyanophyta, Cyanophyceae, Phycomycetales, Oscillatoriaceae, contains a large amount of protein, abundant nutrients and multiple bioactive substances, and also contains 7 vitamins, wherein the content of vitamin B12 is especially high. The spirulina platensis has high digestibility and is an excellent bait.
Dunaliella salina belongs to Chlorophyta, Chlorophyceae, Volvocales, Dunaliella salina in classification, and stores a large amount of organic compounds such as glycerol and beta-carotene, wherein the content of the beta-carotene can account for 8% of the dry weight of cells, and is an excellent bait.
The clostridium ethanolate protein is a novel mycoprotein produced by taking clostridium ethanolate as a fermentation strain, has the crude protein content of more than 80 percent, is easy to digest and absorb by animals, is rich in various trace elements, does not contain anti-nutritional factors, is rich in 18 amino acids, has the content of total amino acids in the protein of more than or equal to 85 percent, and has relatively higher lysine content and sulfur-containing amino acid content.
The compound vitamin additive is a special nutrient component for providing vitamins and trace elements for shellfish organisms.
The sodium caseinate is an important protein raw material, has good emulsification and thickening effects, and is widely applied to the food industry.
Corn starch is a nutrient stored in corn, mainly in seeds and tubers, and has a starch content of about 65% to 72%. From the chemical structure, the corn starch is a high polymer of glucose, is a polysaccharide, can be decomposed into sugar with a simpler structure under a certain condition, can be completely hydrolyzed to obtain glucose, can provide a large amount of energy for animals, and is an indispensable important nutrient component for fattening oysters.
The preparation method comprises the following steps: weighing raw materials → preparing oil phase liquid and preparing water phase liquid → mixing evenly → emulsifying → filtering → homogenizing under high pressure → vacuum degassing → spray drying → cooling granules → sieving. The original production processes of crushing and superfine crushing are reformed into the production processes of emulsification, high-pressure homogenization and spray drying, and the prepared oyster compound feed rich in fish oil has a spatial stereo form of microcapsule globules with the particle size of about 10 mu m, thereby meeting the requirement of oyster on the particle size of food particles within 25 mu m; experiments prove that the oyster compound feed rich in fish oil can keep a suspension state in seawater for 7 days, and effectively avoids feed waste. And a layer of sodium caseinate film is coated outside the microcapsule pellets, so that the air isolation effect is achieved, and the rancidity of the fish oil is prevented; and the sodium caseinate film is insoluble in water, so that the problem of water absorption and expansion of feed particles in the prior art is effectively solved, the loss of feed nutrients is avoided, and the swelling rate of the fish oil-rich oyster compound feed is less than 10%.
The preparation method of the invention emulsifies the fish oil with sucrose fatty acid ester under proper conditions to form fish oil emulsion, then evenly mixes the fish oil emulsion with water phase liquid, emulsifies the fish oil emulsion by an online high-speed shearing emulsifying machine and homogenizes the fish oil emulsion by a high-pressure homogenizer to form oil-in-water microcapsules, and the grain diameter of the microcapsules is about 2 mu m; after spray drying by a spray dryer, the fish oil micro-capsules with the grain diameter of about 2 mu m are wrapped in the oyster compound feed with the grain diameter of about 10 mu m, thereby achieving the purpose of uniform mixing.
Detailed Description
The present invention will be further described with reference to specific examples to assist understanding of the invention. The method used in the invention is a conventional production method if no special provisions are made; the starting materials used, unless otherwise specified, are conventional commercial products.
Example 1
(1) Weighing 4 parts of fish oil, 5 parts of Isochrysis galbana, 15 parts of schizochytrium, 15 parts of spirulina platensis, 20 parts of dunaliella salina, 10 parts of clostridium ethanolate protein, 0.5 part of a vitamin complex additive, 0.5 part of lipase, 0.5 part of sucrose fatty acid ester, 5 parts of sodium caseinate and 24.5 parts of corn starch according to parts by weight;
(2) adding fish oil into an oil dissolving pot, heating to 70 ℃, adding the sucrose fatty acid ester weighed in the step (1), and stirring for 30 minutes at constant temperature to obtain oil phase liquid;
(3) adding water 7 times the total weight of the raw materials in the step (1) into an emulsifying tank, heating to 70 ℃, adding the corn starch weighed in the step (1), and stirring at constant temperature for 30 minutes to obtain a corn starch solution;
(4) sequentially adding the isochrysis galbana, schizochytrium limacinum, spirulina platensis, dunaliella salina, clostridium ethanolate protein, a vitamin complex additive, lipase and sodium caseinate weighed in the step (1) into the corn starch solution obtained in the step (3), and stirring at constant temperature for 5 minutes to obtain an aqueous phase liquid;
(5) adding the oil phase liquid obtained in the step (2) into the water phase liquid obtained in the step (4), and stirring for 10 minutes at constant temperature to obtain a crude mixed liquid;
(6) emulsifying the crude mixed liquid obtained in the step (5) by using an on-line high-speed shearing emulsifying machine to obtain fine mixed liquid;
(7) filtering the fine mixed solution obtained in the step (6) by using a duplex filter, wherein the mesh number of a screen is 200 meshes to obtain filtered mixed solution;
(8) homogenizing the filtered mixed liquor obtained in the step (7) by a high-pressure homogenizer at the pressure of 60Mpa to obtain fine mixed liquor;
(9) performing vacuum degassing on the fine mixed solution obtained in the step (8) by using a vacuum degassing pot to obtain degassed mixed solution;
(10) spray drying the degassed mixed solution obtained in the step (9) by using a spray dryer, wherein the air inlet temperature is 130 ℃, and the air outlet temperature is 85 ℃ to obtain a spray-dried semi-finished product;
(11) introducing hot air into the front half part of the spray-dried semi-finished product obtained in the step (10) for auxiliary drying, and introducing dehumidification cold air into the rear half part of the vibration fluidized bed for cooling to obtain a fluidized bed semi-finished product;
(12) and (4) screening the semi-finished product of the fluidized bed obtained in the step (11) by using a vibrating screen, wherein the mesh number of the screen is 350 meshes, so as to obtain the oyster compound feed rich in fish oil.
Example 2
(1) Weighing 2 parts of fish oil, 10 parts of Isochrysis galbana, 10 parts of schizochytrium, 10 parts of spirulina platensis, 25 parts of dunaliella salina, 12 parts of clostridium ethanolate protein, 1 part of vitamin complex additive, 1 part of lipase, 1 part of sucrose fatty acid ester, 8 parts of sodium caseinate and 20 parts of corn starch according to parts by weight;
the reaction steps (2) to (12) were the same as in example 1.
Example 3
(1) Weighing 5 parts of fish oil, 5 parts of Isochrysis galbana, 20 parts of schizochytrium, 20 parts of spirulina platensis, 15 parts of dunaliella salina, 8 parts of clostridium ethanolate protein, 0.5 part of vitamin complex additive, 0.5 part of lipase, 1 part of sucrose fatty acid ester, 5 parts of sodium caseinate and 20 parts of corn starch according to parts by weight;
the reaction steps (2) to (12) were the same as in example 1.
Example 4
(1) Weighing 6 parts of fish oil, 5 parts of Isochrysis galbana, 12 parts of schizochytrium, 15 parts of spirulina platensis, 15 parts of dunaliella salina, 10 parts of clostridium ethanolate protein, 0.5 part of vitamin complex additive, 0.5 part of lipase, 1 part of sucrose fatty acid ester, 5 parts of sodium caseinate and 30 parts of corn starch according to parts by weight;
the reaction steps (2) to (12) were the same as in example 1.
The particle size of the oyster compound feed prepared by the invention is less than 25 mu m, and the oyster compound feed does not expand when meeting water and has good suspension property.
Oyster feeding and fattening test:
selecting finished oysters with low fullness, cleaning, splitting into single oysters, removing shells of damaged oysters, selecting 500kg of oysters with similar heads and weights, selecting 100 oysters in addition, dividing all oysters into 5 groups, selecting 20 oysters in each group of 100kg + 100 oysters in addition, weighing and dissecting 20 oysters in each group, taking out flesh parts, weighing, calculating the average value of the initial fullness of 20 oysters, and estimating the average value of the initial fullness of each group of oysters according to the average value. The oysters of the control group are placed into 2 breeding cages and are put into the same sea area, only the natural baits in the seawater are eaten, and no additional feed is fed. And (3) respectively putting each group of oysters of the test group 1-4 into 10 flat turnover boxes, and putting the flat turnover boxes into an independent oyster purification pool. Test group 1 was fed with the oyster compound feed prepared in example 1, test group 2 was fed with the oyster compound feed prepared in example 2, test group 3 was fed with the oyster compound feed prepared in example 3, and test group 4 was fed with the oyster compound feed prepared in example 4. The test groups 1-4 feed the animals at 7 am and 19 pm every day, and the feed amount is increased gradually according to 2% of the dry meat weight. Simultaneously, sufficient oxygen supply is kept, water is changed for 1 time every day, the test duration is 30 days, 20 samples are randomly taken from each group, and the average value of the end fullness of each group of oysters is estimated according to the average value of the end fullness of 20 oysters; and the mortality rate of each group of oysters is counted respectively. The results are given in table 1 below.
Average fullness (%) is flesh weight/total weight x 100%
The ratio of the increase of the fullness is the average of the end fullness/the initial fullness
Mortality (%). mortality-number of dead oysters/total number of oysters
TABLE 1 oyster fullness and mortality statistics
As can be seen from the fullness growth ratios of oysters in the table 1, the fullness growth ratios of the test groups 1-4 are superior to those of the control group, and the mortality is lower than that of the control group, so that the oyster compound feed rich in fish oil has an obvious effect on short-term fattening of oysters, and the survival rate of the oysters in the growing and fattening period is improved.
In conclusion, the oyster compound feed rich in fish oil has the advantages of simple and reasonable raw material composition, natural and environment-friendly components, scientific proportion, balanced nutrition and remarkable fattening effect, and the oyster compound feed rich in fish oil prepared by the invention has the advantages of particle size of less than 25 mu m, no expansion when meeting water and good suspension property.
Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that the embodiments may be modified or changed without departing from the spirit of the present invention within the scope of the claims.
Claims (3)
1. The oyster compound feed rich in fish oil is characterized by comprising the following raw material components in parts by weight: 2-6 parts of fish oil, 5-10 parts of Isochrysis galbana, 10-20 parts of schizochytrium limacinum, 10-20 parts of spirulina platensis, 15-25 parts of dunaliella salina, 8-12 parts of clostridium ethanolate protein, 0.5-1 part of vitamin complex additive, 0.5-1 part of lipase, 0.5-1 part of sucrose fatty acid ester, 5-8 parts of sodium caseinate and 20-30 parts of corn starch.
2. The oyster compound feed rich in fish oil according to claim 1, which comprises the following raw materials in parts by weight: 4 parts of fish oil, 5 parts of Isochrysis galbana, 15 parts of schizochytrium, 15 parts of spirulina platensis, 20 parts of dunaliella salina, 10 parts of clostridium ethanolate protein, 0.5 part of vitamin complex additive, 0.5 part of lipase, 0.5 part of sucrose fatty acid ester, 5 parts of sodium caseinate and 24.5 parts of corn starch.
3. A method for preparing the fish oil-enriched oyster compound feed as claimed in claim 1 or 2, comprising the steps of:
(1) respectively weighing fish oil, Isochrysis galbana, schizochytrium, spirulina platensis, dunaliella salina, clostridium ethanolicum protein, a vitamin complex additive, lipase, sucrose fatty acid ester, sodium caseinate and corn starch in parts by weight;
(2) adding fish oil into an oil dissolving pot, heating to 70 ℃, adding the sucrose fatty acid ester weighed in the step (1), and stirring for 30 minutes at constant temperature to obtain oil phase liquid;
(3) adding water 7 times the total weight of the raw materials in the step (1) into an emulsifying tank, heating to 70 ℃, adding the corn starch weighed in the step (1), and stirring at constant temperature for 30 minutes to obtain a corn starch solution;
(4) sequentially adding the isochrysis galbana, schizochytrium limacinum, spirulina platensis, dunaliella salina, clostridium ethanolate protein, a vitamin complex additive, lipase and sodium caseinate weighed in the step (1) into the corn starch solution obtained in the step (3), and stirring at constant temperature for 5 minutes to obtain an aqueous phase liquid;
(5) adding the oil phase liquid obtained in the step (2) into the water phase liquid obtained in the step (4), and stirring for 10 minutes at constant temperature to obtain a crude mixed liquid;
(6) emulsifying the crude mixed liquid obtained in the step (5) by using an on-line high-speed shearing emulsifying machine to obtain fine mixed liquid;
(7) filtering the fine mixed solution obtained in the step (6) by using a duplex filter, wherein the mesh number of a screen is 200 meshes to obtain filtered mixed solution;
(8) homogenizing the filtered mixed liquor obtained in the step (7) by a high-pressure homogenizer at the pressure of 60Mpa to obtain fine mixed liquor;
(9) performing vacuum degassing on the fine mixed solution obtained in the step (8) by using a vacuum degassing pot to obtain degassed mixed solution;
(10) spray drying the degassed mixed solution obtained in the step (9) by using a spray dryer, wherein the air inlet temperature is 130 ℃, and the air outlet temperature is 85 ℃ to obtain a spray-dried semi-finished product;
(11) introducing hot air into the front half part of the spray-dried semi-finished product obtained in the step (10) for auxiliary drying, and introducing dehumidification cold air into the rear half part of the vibration fluidized bed for cooling to obtain a fluidized bed semi-finished product;
(12) and (4) screening the semi-finished product of the fluidized bed obtained in the step (11) by using a vibrating screen, wherein the mesh number of the screen is 350 meshes, so as to obtain the oyster compound feed rich in fish oil.
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CN114917599A (en) * | 2022-05-07 | 2022-08-19 | 常州市淮海干燥设备有限公司 | Florfenicol preparation process based on spray dryer |
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CN114917599A (en) * | 2022-05-07 | 2022-08-19 | 常州市淮海干燥设备有限公司 | Florfenicol preparation process based on spray dryer |
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